LOCUS       CP066210                5201 bp    DNA     circular BCT 26-MAY-2021
DEFINITION  Enterococcus faecium strain 18-204 plasmid p18-204_5, complete
            sequence.
ACCESSION   CP066210
VERSION     CP066210.1
DBLINK      BioProject: PRJNA682333
            BioSample: SAMN16988098
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Dejoies,L., Sassi,M., Schutz,S., Moreaux,J., Zouari,A., Potrel,S.,
            Collet,A., Lecourt,M., Auger,G. and Cattoir,V.
  TITLE     Genetic features of the poxtA linezolid resistance gene in human
            enterococci from France
  JOURNAL   J Antimicrob Chemother (2021) In press
   PUBMED   33895846
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5201)
  AUTHORS   Dejoies,L., Sassi,M., Schutz,S., Zouari,A., Potrel,S., Collet,A. and
            Cattoir,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2020) Rennes1 University, U1230, 2 avenue du
            Professeur Leon Bernard, Rennes 35043, France
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: OCT-2020
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 250x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/16/2020 11:29:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.0
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 2,926
            CDSs (total)                      :: 2,836
            Genes (coding)                    :: 2,412
            CDSs (with protein)               :: 2,412
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 424
            CDSs (without protein)            :: 424
            Pseudo Genes (ambiguous residues) :: 0 of 424
            Pseudo Genes (frameshifted)       :: 369 of 424
            Pseudo Genes (incomplete)         :: 48 of 424
            Pseudo Genes (internal stop)      :: 24 of 424
            Pseudo Genes (multiple problems)  :: 16 of 424
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="18-204"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p18-204_5"
                     /geo_loc_name="France: Creteil"
                     /lat_lon="48.7989 N 2.4658 W"
                     /collection_date="2018-04"
                     /collected_by="NRC for Enterococci"
     gene            334..615
                     /locus_tag="I8F51_14590"
     CDS             334..615
                     /locus_tag="I8F51_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QVX03365.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESDF
                     DRQLMNYKKDQFDLLLEKFIDIQTKNTETLNDYVHTNNQIIETLIGGI"
                     /name="hypothetical protein"
     gene            617..1033
                     /locus_tag="I8F51_14595"
     CDS             617..1033
                     /locus_tag="I8F51_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QVX03366.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSYE
                     PNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKIEK
                     QLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(1480..2499)
                     /locus_tag="I8F51_14600"
     CDS             complement(1480..2499)
                     /locus_tag="I8F51_14600"
                     /inference="COORDINATES: protein motif:HMM:NF019371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="QVX03367.1"
                     /translation="MKTSNRKYKVKTVNHRIPPKKALCVINNSKVINQELKPYLSEQEM
                     IKKISKDIFLTNSDTILLEKFLKKNSYFRFSIYVKLMKNIDKVTILDVIETYKLDSFIR
                     NQLHYFVNQIEIFWKKSLSDNMCVSYEETSIFPKNQCYLDKNIYSDLKWAEDIIGHFNS
                     FFYSNQSPTFKHHHIKKNHCLPIWALFEEITFGSLTTFINQLNTEYYNNWVMSCYDNPK
                     YKKAIRSWANVIRAYRNKIAHHSRIYAFKVTDAPTIIKNDSKSYFPENNLKETQKLYLY
                     GGLYVIKHLLIYEDSMTQTLWNKFLIDLEKKIDKIPSLEKSNYGFPENWRNKLKIMIV"
                     /name="Abi family protein"
     gene            3649..4617
                     /locus_tag="I8F51_14605"
     CDS             3649..4617
                     /locus_tag="I8F51_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QVX03363.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQF
                     TFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKIDGK
                     ADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIAYFS
                     KEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGYQFTF
                     KPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELLQQLEK
                     LEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
                     /name="replication initiation protein"
     gene            4635..5159
                     /locus_tag="I8F51_14610"
     CDS             4635..5159
                     /locus_tag="I8F51_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="QVX03364.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINET
                     GQKAIKSMFFNENENSNQQLVVEKTKTENHKNANQVPQLEVLSLLEQQLNQKDMQLQEK
                     DEQIKQMQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTILSEETESIEKQS
                     KTEKKWWQFWQ"
                     /name="DUF536 domain-containing protein"
     Typing          3650..4613
                     /name="rep_cluster_1093"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.661"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ctttttgctt ttgcgagcag agcaacatgg gcccatgttg cgatattttg gtggcgcccc
       61 aatccccgtt ttttcgttga atcttactcg gactgcactc gttttacagc tgatttttct
      121 gaattttgaa agagcataag gaaacgtggt atcactttct tacgaaaggt gctaggtagg
      181 ctgtgcctac cacgtttcta agaaaaaaat ttgctatcac tttttcgcca ttatggatag
      241 cttatgtata gtgcttcggc aaaaaacagc tatacaaata tagtatttgt atgatataca
      301 taaattatac ataattgcaa aaggagattt tttatggaca ttttagttcg gaatattgat
      361 ccaaagtttg ttgcaacaat agacaaacgt tgccaagaac tcacgaataa aacaggagaa
      421 aaatggagtc gaaataaata tctaaaagtt ctaatcgaaa gtgattttga ccgtcaactt
      481 atgaattaca aaaaagatca attcgattta ttactcgaaa aatttatcga tattcaaaca
      541 aaaaatactg aaacattgaa cgactatgtt catacaaaca accaaatcat tgaaacactt
      601 atcggaggaa tataacatga gaaaaacttc tgttcaaaaa cacgttaccc tttatcccag
      661 tcacattgac gttttagatt ccataatcga aaaaaatcca gatatcaata actactcgga
      721 agccattcgt tttctatgta tgagttatga acctaatact aatacgacaa ctaccagcaa
      781 ccaagcaaaa ctaaatgcga tgggaaaaga aatttctatt atgtccgaaa tcattgcaga
      841 atttgctgat ttgtatttaa aagatacagg cgttcttatc ggtacaaact cgaaagtata
      901 tcaagaagta aaagaaaaaa ttgaaaaaca gctaaatccc tcccaacgaa atcaaccaaa
      961 gaatcgtcaa aaagaaacga gtcaaacatt cacttctaaa aatttcgacc caaccaactt
     1021 tctaaaaaga taaaaaaata aaccaggaaa ttaaaaattt cctggtttat tttctagaga
     1081 gcggtcttcc aataagattt gcaaatcgat cccaatttgc gtaaaaaaaa aagctgctcg
     1141 tacgagcagc taaacaagaa aaagttagct tgttttaagg gtaaaataag tgtaacaaat
     1201 tttgcgaatg taacgatctt ttttattata cgcaaacaat caaaaaaaaa gactgctcga
     1261 aagagcagtc tcacaagcgc tcacaaaaat aacaaccccc taaaaacaga ccggtccaaa
     1321 ttcacaaaga aatggaagaa acaaatcgta tcaaatcaag ctttagaaag tctcttttag
     1381 ctagaccaat gaaaaatcac aaagtgagcg tctctatcca ttggtagagg ggcaacttgg
     1441 tctagtgtac attatgttaa ctaacaattt aaataacaat taaacaatca ttatttttaa
     1501 cttgtttctc caattttccg gaaaaccata attggatttt tctaaggaag ggattttatc
     1561 gatctttttt tctaaatcta ttagaaattt attccataaa gtttgtgtca tgctatcttc
     1621 atatattaac agatgtttta taacgtataa tcctccatat aaatataact tttgggtttc
     1681 ttttaagtta ttttcaggaa aataactttt ggaatcattt tttatgattg ttggagcgtc
     1741 agtaacttta aatgcatata ttcttgaatg atgagctatc ttatttctat aagctctaat
     1801 tacgtttgcc caacttcgta tagctttttt gtatttagga ttatcataac agctcatgac
     1861 ccaattatta taatactctg tattaagttg attaataaat gttgttaaac taccaaatgt
     1921 tatttcttca aataaagccc atattggtaa acaatgattt ttttttatat gatgatgttt
     1981 aaaagtggga ctttgatttg aataaaaaaa actattaaaa tgaccaatta tatcttcagc
     2041 ccatttcaaa tctgaataaa tatttttatc caaataacac tgattcttcg gaaatatgct
     2101 agtttcttca taagaaacgc acatattatc tgaaagagat tttttccaaa aaatttctat
     2161 ttgattcaca aaataatgca attgattacg aatgaatgaa tctaatttgt acgtttcaat
     2221 aacatctaga atagtaactt tatctatatt tttcattaat ttaacataaa tactaaatcg
     2281 aaaataacta tttttcttta aaaacttttc taataaaatt gtatcggaat ttgttaaaaa
     2341 aatatcttta cttatttttt ttatcatttc ttgctcggat aaatatggtt ttaactcttg
     2401 attaattact ttggaattat taattacaca caatgccttt ttaggtggaa ttctatgatt
     2461 aactgtttta actttatatt ttctatttga tgtcttcata agaatcttct ttcttaaaaa
     2521 aatataaaaa aaaagcccta ccataatgcg catgatatta aatcagaggc gtggcaagta
     2581 ctgatagata tattaaaaca catttgaatt aaactgtaaa gcttttttat ttaaaaattt
     2641 agttgtcaaa atcgtgattt tttttaagtg tgcaaccaaa agaaatgcct atatatcaag
     2701 cattactatc ataatttttc acattatccc ccccctgtta gaatgcgggg ggctaaatgc
     2761 gatcggtcta tcgagcaagc tcgatagacc tttttttgct tgaaaatggc gcaaaaccgg
     2821 tgcctttcac gaatttgtaa gcttgtttgt acacgtctcg ctctaggaga ccttgttcgc
     2881 tccgcaccgc aagcagcttt tagcaacagc acttttttgc taacgtacga aaatggctct
     2941 tccggaattc tgctgaatcg gttgcttatt gttttgtttc atacgctctt atttgcgttt
     3001 tcagacgttt tactacccaa ggtatataaa atatacaaga acatagataa gacagattaa
     3061 atcgaatttt aggatggttt tatgctcttt aagggttgtt agggcttgcc ctgaccgcct
     3121 gtaaggtgct ttaagaacaa gataagggtt tatccatact aaactgtctt aaaacggctt
     3181 aaacgagcgc gcagagcctc taaaaaaatc tctttaaaca acgtttttag caaaggagcc
     3241 tttaggcgac tgcgcaggta caatgtgagg gtgtttttcc ctcacacctt tttagcgacc
     3301 agagggagca agtttcctag tttagtaaaa ttcgcgaacc tgtttggcta aaaaagcaga
     3361 aaatttttag gcgggaaatt ttgcccctct cctcttttat aatctctttt taaacctctt
     3421 tttaaacctc tttaccgtcg gctgtatgct tactctccca aggtgtgcag aatcttttgg
     3481 gggtcttttt gtctgttttt aggggtcttt ttgtctgttt ttaggggtct ttttgtctgt
     3541 ttttaggggt ctttttgtct gtttttaggg gtctttaatt aggggcttga cccctattag
     3601 aaagctcttt ataattttgt taagtagtta atcttgaagg aggattttat ggcaaatgaa
     3661 attgtgaaat accaccatga attaaatacg attcctttac gcaaatttac gtcagtagaa
     3721 atgaatttat tcttctctat agtatctaga atgcgtgacg taggcgataa aaaagtacaa
     3781 tttacattcg aacaattaaa agatttaagc aattataaag caactgcaaa tgttcgattc
     3841 atagatgatt tagaaacaac gtatgacaaa ttaatggatt tacgttttgg tagaagaagt
     3901 gctgacggtt tgcaacgtga acgatttgtt ttatttaatc aatttaaaat cgatggaaaa
     3961 gctgatattc cttttgctga aatacaagtc catgagaaag ccttaccctt acttaataat
     4021 cttgaagaat gggttcgata ttctctccaa caattcaatg aattagaaag cagttattcc
     4081 aaaaccatgt ttagattatt aaaacaatac agaaccaaag gaattgccta tttttcaaaa
     4141 gaagactttc atgaattact tgatatacct aaaagttata gagaaagtga tataaataaa
     4201 aaaattataa agcctattcg ccaagaacta accgcaatct tcaaaggtct gacaatccaa
     4261 aagagatacg gaaaaggacg gggaaagcct gtcattggct atcaatttac atttaaacct
     4321 gagataaaaa aatcagacga ctttaacaaa catgccccaa caaaagaaga acaaaacaaa
     4381 gcattagaac tagctaacca acaagttcaa tctgaaaaag cacctacttc attacaagaa
     4441 aaaatcaaag aacaaaagcg aaaattggaa cttttacaac agttagaaaa gttagaaaga
     4501 gaacaaaata cccaaaataa agaaaataat gcacaaaaat cagagataaa ttccgatatt
     4561 ccttcagaac taaaaaaaga cttattagac aatcttcaaa acttattcaa agattgaggg
     4621 ggtttttaga ttttatgaca gaaaaaacaa tcaaagaact tgctgaagaa ttaggagtta
     4681 gtaaaacagc aattaacaaa aaagtttctg acgaaaatcg aaaactttgg ttttcgaaaa
     4741 tcggaaacaa gtttgtgatt aatgaaaccg gacaaaaggc tataaaatca atgtttttta
     4801 acgaaaatga aaactcaaat caacaactag ttgtcgaaaa aacgaaaacc gaaaaccaca
     4861 aaaacgccaa ccaagttccc caattagaag tcctttctct cctagaacaa cagttaaatc
     4921 aaaaagatat gcaactacaa gaaaaagacg agcaaataaa acaaatgcaa aaattactcg
     4981 accaacaaca acaactcacc ctgcaagcca accaacaaat cgagaaactg caggaacaac
     5041 tacagttgac ttatacggaa gaatcttcgg aaaataaatc aacgattctt tctgaagaaa
     5101 cagaatcgat agaaaaacaa tcaaagacgg agaaaaaatg gtggcaattc tggcaataaa
     5161 accagcaatt taaattaatt taaattgctg gttttattct t
//
