LOCUS       CP064291                9523 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  Enterococcus faecium strain Cairo plasmid pEFAEC_CAIRO_frag10.
ACCESSION   CP064291
VERSION     CP064291.1
DBLINK      BioProject: PRJNA672694
            BioSample: SAMN16574827
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9523)
  AUTHORS   Broddrick,J.T., Szubin,R., Norsigian,C.J., Monk,J.M., Palsson,B.O.
            and Parenteau,M.N.
  TITLE     High-quality genome-scale models from error-prone, long-read
            assemblies
  JOURNAL   Front Microbiol (2020) In press
REFERENCE   2  (bases 1 to 9523)
  AUTHORS   Broddrick,J.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2020) Exobiology Branch, NASA, Bldg N239 Rm 315,
            Moffett Field, CA 94035, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date          :: 20-APR-2019
            Assembly Method        :: Flye v. 2.6; Racon v. 1.4.7; Medaka v. 0.9
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 46x
            Sequencing Technology  :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="Cairo"
                     /isolation_source="clinical isolate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pEFAEC_CAIRO_frag10"
                     /geo_loc_name="Egypt: Cairo"
                     /collection_date="06-Jul-2018"
     ARG             complement(2..891)
                     /name="ant(6)-Ia"
                     /gene="ant(6)-Ia"
                     /gene_name="aminoglycoside nucleotidyltransferase
                     ANT(6)-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="98.34"
     ARG             complement(4155..4888)
                     /name="erm(B)"
                     /gene="erm(B)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(2..891)
                     /name="aad(6)"
                     /gene="aad(6)"
                     /gene_name="ANT(6)"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="107.61"
     ARG             complement(4152..4888)
                     /name="ErmB"
                     /gene="ErmB"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="98.37"
                     /coverage="98.79"
     Typing          complement(6539..7578)
                     /name="rep_cluster_889"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttctatatca gcggcatatg tgctatccag gcagccggtt ttaccagtgt attttttata
       61 catgtccctg gtatattttg ttatattcct atcatactcc ggataggcat aatgaagcct
      121 ttccgccacc tcaccggata ccgccctgaa caattgatgg catagaaata atgcttccca
      181 tatgttttca taggaatcca tccggtaggt ggacaaaagt ttctcccaca aatcctcgga
      241 tatatacctt tcaataaact tatagttctt gcctacactt aatttaaagc ctgtttcgat
      301 gccgaccttc catgatatca ttctcagcag ctcatggcga acaatctgat taaaatgatc
      361 aatagcaaat aaaatttcct tacggcacaa tcctttaata acataaggtg ttacattcca
      421 aaattcattg cagcaatcat catactccct tgcgcttggc tttcttacat gataatctat
      481 atcagtcgga actatgtccc ttttaattct acaatcttta tcaattagaa cctttattaa
      541 tttatcgccc tttaggtaat tatctaactc ttccaagggc aataaggtaa gatcaatttt
      601 attgtaatca tcaaatagca taagatagga aaatcccttt tcttcaggtg ggaataattc
      661 catatcctcc ggcttttgca tcattattat attcccaaat tgattaagcc agtcatcatt
      721 agatataaac ggttctatat cacttacaaa atatgtaata tcataatcct gaaattcatc
      781 tttaggtata ttaatatttg cgcgtgaccc ctcaagggtc acaattcgaa tacgttcatc
      841 ctgttctgct aaagaaagta ctaaatccat catttctttt tctgatctca tttacatact
      901 cctcgtttat tttttctata ttattacatc ttcttttttg ccgatacaat cagcatcatt
      961 gggcgtcgca tttcatccgc catccccgga atatccatca tgttctctgg cggctgtggc
     1021 tccacaatct gatttattat aaaactattt gaaagcagtg tatttagata tgtggtcagt
     1081 gttctatgat attttgtaac cttttcttcc aaaaacatag ctgtccgttt gccctcataa
     1141 taataattgt ccaccgggaa atgcagtatt tctccttttt cgttataata ccagtcttgt
     1201 gttccatgag cagtaaaaac aggatgttca actgtaaaaa ctaaattgcc accagccttc
     1261 agcatcctat atatcttttt tattaaattc tcatagtctg ctacataatg aaacgcaagc
     1321 gaacttagta ttacatcaaa gctctcctct gggaaatcca catcttctat ggcacagcat
     1381 tcatattcaa tctgtggaaa atgggttttt ccttttgcta cttcgagcat tttatgagaa
     1441 atatcaacac ctactacaga ggaagcaccg ttttccatcg catatataca gtgccatcca
     1501 tagccgcatc ctaaatcaag cacacgctta cccttaaaat caggtagcat ctttttcaaa
     1561 gtctcccatt ctcccgcacc agccagtcct ttctgcgagc gactcatttg actgtatttt
     1621 tgaaaaaata tattatcatc atatttgttt tctttcatct gaactctcct cgtttaaaaa
     1681 gttatttatc tccgatacaa tttcattcac ttcattataa agcttctcgg tcatgtcgta
     1741 gcattcaaaa agtgagatac cgagtacttc aaaaatatga tttaccttct cggtatattt
     1801 ttcaggttta tgttcaaaag tttcaagcag ttttatagct ttcttttcgt tgatacaata
     1861 agcattatta catgcaaata acacttgatt taaacatgaa actatacgaa aaacatgacc
     1921 cgcaatataa tatttatcgt ctgttcccga atttgctttt acaaacatta aagagaaccc
     1981 tgcttcaaac ataaaaaagt taactaaact tttctgcaaa gcattgggat aagtttctgc
     2041 ctgttttttt aattcgcata agctttcatt cttagcatat agtattttgc taatcgctaa
     2101 ttctcctcga tacattgcac taatataacc atggggatgc ccagtctgat aattggcagt
     2161 aacaattccg tgctctgtat ctttcattat ttgttccaca cgtttaatat cacgtaaaat
     2221 taaatccaca tgatacccgt ttatgactaa ccatccgccg ccattaatcc aatcacccca
     2281 tgctccggga ggtacaacaa ggttatttct atgctcatca tccagctttg tagcgaattg
     2341 attaatagta tttatgtcaa atgattctga attgtaatag atgccgatat ctatatccga
     2401 atcctctgta tgggtgcccc ttgcacgtga accacctaaa acaatacctt ctatataaga
     2461 cagagaggat aatttctctg ctactgattt aataatatta tctaccatca agcatgctcc
     2521 ttttatcgat tcactgctgt aaaaattcca agccaaatct tttgcttcta ctcaacatat
     2581 ttataatttt tttaatttca caaaagatag ttaaaactta tcaattgtct ttttatacct
     2641 ctgctgatta gatgaagtct tttcaatagc ctttaaatta ccattcattt tgctaacatg
     2701 gtttaaaaat aacgttccaa gcagaagtcg atcctttctg ggtatcctat tccccacata
     2761 tcccttgaat aaatctttaa caagaaaaac ttcgccatca tttagatttt cagtttctct
     2821 aatagcttct tccaatagct catttacatc aatcatgttg tccactcctt attaataatg
     2881 ggtcagcccc cttctactgt ttcggtttgg tcaatcgctt caattaattg attttccttt
     2941 tcttccaaag attgacgaat cctttcaatc tcttctatag aaagttcatc ctttaaatca
     3001 tctattaact caggtaagat ttttgtttcc accatttgtt ggtagttttc aagggctttt
     3061 tgatcaaggc tatccttata ctttaaaata ctttttttcc aaacgaaaga gtatttattc
     3121 gcatccgctt caatttttgt accatcaatg tatagagcac tgccactgat taagccactt
     3181 tgaaccagta gctcacgaaa ttgaacaaat gaatgatcta gaatatcgtt cataatcgga
     3241 tgaacgcgaa aacgattaat agtacgaaag ttagggaatt gctcttgaga taagtagcgc
     3301 atccgaatac tatctatcag catattttgt atttggcggc ccgaataaac cccttgaata
     3361 taggcacaaa gaataatgga aagtagcata cgaggatgat attggggtct acccatagat
     3421 tgataataag gaagaaagac ttcatcggga atagagtcaa caaactgcac aatggcatga
     3481 gcaaaatcat tttcttctaa tttaatttca agatctatag gcaaacaaag ttggtttatg
     3541 gtataatttt gatacatgat aaagcacctc tctcaaatgg attaatcacc tatattatag
     3601 cagagtgtgc tttatttttg tattcacttt caaaaagata aatagaggtc tttccactac
     3661 tatctaaatg gaaagacctc tatttttcat ataggagcta cttttgggtc agccccatgt
     3721 ttattttgaa aaagagataa aatcaatata tcccttttcc ccaattttta caacggcatt
     3781 gtagggcttt ttctctttgt ttttgattcc ttttaccagg gtttcttttc cctccagtaa
     3841 ttcttttaca ttcgttttgg ttagtttttt ctttctaaaa tgttcagcta aagtaaactt
     3901 acattcagga taatttgaac aaccataaaa cgattttttt aatacaatat tgttgccaca
     3961 cttaggacat tttcctacaa ggggtcccga gcgcttagtg ggaatttgta ccccttatcg
     4021 atacaaattc cccgtaggcg ctagggacct ctttagcttc ttggaagctg tcagtagtat
     4081 atctaataat ttatctccat tccctttagt aacgtgtaac tttccaaatt taaaaaagcg
     4141 actcatagaa ttatttcctc ccgttaaata atagataact attaaaaata gacaatactt
     4201 gctcataagt aacggtactt aaattgttta ctttggcgtg tttcattgct tgatgaaact
     4261 gatttttagt aaacagttga cgatattctc gattgaccca ttttgaaaca aagtacgtat
     4321 atagcttcca atatttatct ggaacatctg tggtatggcg ggtaagtttt attaagacac
     4381 tgtttacttt tggtttagga tgaaagcatt ccgctggcag cttaagcaat tgctgaatcg
     4441 agacttgagt gtgcaagagc aaccctagtg ttcggtgaat atccaaggta cgcttgtaga
     4501 atccttcttc aacaatcaga tagatgtcag acgcatggct ttcaaaaacc acttttttaa
     4561 taatttgtgt gcttaaatgg taaggaatac tcccaacaat tttatacctc tgtttgttag
     4621 ggaattgaaa ctgtagaata tcttggtgaa ttaaagtgac acgaatgttc agttttaatt
     4681 tttctgacga taagttgaat agatgactgt ctaattcaat agacgttacc tgtttactta
     4741 ttttagccag tttcgtcgtt aaatgccctt tacctgttcc aatttcgtaa acggtatcgg
     4801 tttcttttaa attcaattgt tttattattt ggttgagtac tttttcactc gttaaaaagt
     4861 tttgagaata ttttatattt ttgttcatgt aatcactcct gaagttatta catctgtaaa
     4921 taaatacaga agttaaacga tttgtttgta attttagtta tctgtttaaa aagtcataag
     4981 attagtcact ggtaggaatt aatctaacgt atttatttat ctgcgtaatc actgttttta
     5041 gtctgtttca aaacagtaga tgttttatct acattacgca tttggaatac caacatgacg
     5101 aatccctcct tcttaattac aaatttttag catctaattt aacttcaatt cctattatac
     5161 acaaaatggt tctgttgcaa agttttaaat ctactatcaa ataaggtaga ataatagaaa
     5221 aagatagcag gaggaatgac gatgaatcat tttaaaggaa agcaatttca gcaggatgtg
     5281 attattgtag ccgtgggcta ctatcttcgt tataacctta gctatcgtga agttcaagaa
     5341 atcttatatg atcgtggcat taacgtttct catacgacga tttatcgttg ggtgcaagaa
     5401 tatggcaaac tactctatca aatttggaaa aagaaaaata aaaaatcctt ttattcatgg
     5461 aaaatggatg aaacgtacat caaaattaaa ggaaaatggc attatttgta tcgagccatc
     5521 ggtgcagatg gtttaacctt ggatatttgg ttacgtaaaa aacgggacac acaagcagcc
     5581 tatgcttttc ttaagcggtt agtgaagcag tttgatgaac cgaaggttgt agtcacagat
     5641 aaagccccct ctattacaag tgcctttaag aaactaaaag aatacggctt ttatcaaggg
     5701 acagaacatc gtaccattaa atacctgaat aatttgattg aacaagacca tcgtccagta
     5761 aagagacgca ataaattcta tcgaagttta cgcactgcct ctaccacgat taaaggcatg
     5821 gaagccattc gaggattata taagaaaacc cgaaaagaag gcactctctt cgggttttcg
     5881 gtctgtactg aaatcaaggt attattggga atcccagctt aaatcataga taccgtaagg
     5941 gattttattc tttatttaaa actttgcaac agaaccgtcc gttgcgggta ataccgtaac
     6001 actgaagggc acgcattaag gcgtcatctt aatgctttcc tgtaggacac tgacggagat
     6061 taccagtctc ccgagcctta caggggcctt tctttttttt gtattttcaa ttatcacaat
     6121 ttatcgtcca aaagtgcaga acgaaaatca attcatcaca agcatatcag acaattaaat
     6181 ttaaaacaac taaaaatata caaaaaagtg taattgaata ttagcagcac tgcctgaaaa
     6241 gaagtaatat caggcttttc agcttgggga aataagtgaa ttacctctct tcatgaatta
     6301 ttataactca aaaaagcaca tatgcaaaca ctagttccct atttttttga atgaaaaata
     6361 actaaaatat ctaacgatta tgactttaaa acctacgttt gtgattgttt tcataaactt
     6421 ttaatctaaa aactccggaa taatagctat ttatttgtaa catttgtaat aaaattaaaa
     6481 tcataaaaaa gttctaattt aaaattgatc atttttatag tgatcgcttt ttatcaacta
     6541 ctctagccaa ttttccgtac taactgttgg taattcgtta aatgtttctc tttgttcgtt
     6601 tgcaagcttt gccgcaatag ctttttcgac aaaccaattt tttacataaa cgaaaattag
     6661 attctgcaaa ttgtttactt tttcagtttt ttgtttttga taagctttca gcaaagtcat
     6721 atataatcct ttgtcagcat caattccata agattccagt tcttcataaa caacaactgt
     6781 ttgctctaat tcttcagctt ttttctttgc attatgaata gtttggacgg ttttttgagc
     6841 gtcttcgtaa gtactagaaa atgtttttat caagtttaat acattttctg gtacaaatgt
     6901 atttatactg tcctctttca tgatgtcgac gaagttatct agtaaaactt gattttgtaa
     6961 ctgatcagtt tcagtgtcta ttatgtgtct attagtgtct atagaattag aatagaatag
     7021 attgtctgcg attttcgcaa ttacggttga gtccttgata tgactggatt ttgccgtatt
     7081 tgcgattttc gcaattacgg tggtatcatt gatttctcgc ggttttgccg tatttgcgat
     7141 tttcgcaatt acggtggtat cagtattgtc ttcctcttcc atgattttaa agacgtcttg
     7201 attcgttact tctggcttgc ctaagtacaa taaattaggc atggattcaa tcttaccgtc
     7261 acgtttattt actcttcctc tcttctgttt cagaagacct gctttttcta attccttttt
     7321 gattttagat actttacctt cgcggcattg taacaatttc attaattctt caactgtgaa
     7381 tatgaaaaat atattatcat cagaatctac ccagttattt ttgactgaat aatcaaatcg
     7441 atctttaagt agcatataag caatttttgc atcatttgat agatccatgt atttttcgtt
     7501 agtaaaaaat accttaggca tttgataata tttttcacga taaatttctt ttgatttgta
     7561 ataattaaaa tcattcatag ccaatcctct ttctgtagga tagcgttttg ttcaaaacta
     7621 tgttacaatg aattaaacaa atatatctat ccactatata tttttctact gctgataagt
     7681 cgccaaactt tgttaaagca gtagtctttt tttatctttt tttagtttac cttaaaaaat
     7741 aaattaaagc aaaccgctct atttcaaggt ttaagctcat agtaactata tcacgatatt
     7801 acaataatat tatatcacga tatcgtaata ttacaatatg aagataagcg acttatcagc
     7861 tttgatacta ctgattttta taaaaaaaat atcacgatat tatgatatcg tgatattgaa
     7921 tgtaaattaa attttatttt gtttctttgc tagtttatat ccttcaatca agtattcttc
     7981 aggtatggac tttaatagcg catcttctat aacgtctggt ttacttttgt ctgattcagc
     8041 aatagtcgtc caatatttga gtgctgacat tagaattgga tttatacgaa tgctatctgc
     8101 tgttcttaca ctacttcctt gatcttttat gactttactt atatttttct tggttaattc
     8161 gatttcttct acttcttttg atttaaaatt ctttttattt ttatttccta gtaatctact
     8221 tactgcagct tgactgcgat tatcttctgc catcaaaatc accaacttct tttaaatatt
     8281 cttctgtaaa tgctttatag tcatcttcag ctgttgaggg tttattaaaa ttgattagat
     8341 ctgatccagc agcttgcgct tcgtcaacgg ttattgtcct acgaatataa gttttgaaaa
     8401 tttttgcacc aatgactcta gaaatttcgg ttgctgcaga cgtcatttct ttagaaatgt
     8461 tataacgctt attatactta gtaaatagaa ctcctcgtac ttcaagcttg tcgttcgtat
     8521 attcttttac cgtttcaata gtttcactta actttattag tccttttaat gagaaatacg
     8581 cagcttcaac tggaatgata atttcatcag aaacagtaaa tgcgttgact gtaagcattc
     8641 ctaaacttgg tggacaatca attataatat aatcatagtt gtccttgatt ggttgaagta
     8701 ctcttttaag cctgtgttca cttcctactt gagtaaattc cctctctgca gaagaaaggg
     8761 ctatgtcggc tggcaaaaca tcaattcctt tatagtccag aatagcatct gtaatgtcag
     8821 cctccccttt taatacttca taaattgtag gttggtttgt tccatctata tcgaaaccta
     8881 cattatctgt agcatttccc tgaggatcaa tgtccactaa aagtacattt ttaccgtaaa
     8941 caaatttcaa acatgctgct aaattaacgc tagtcgtagt tttagaaact ccaccttttt
     9001 gattggctac tgacaatatt cttgccatta aaatatcccc cattcatctt atattataat
     9061 ataattatat cataatattg tgatattgca atatcgtata tgcattatat tgtaatattg
     9121 tgtgattatt ttgttttaaa gatatatatc ggttcggata taaagattat tcatatgtta
     9181 ttcgaaatta aaataatcac tttttattac tttaaaattt tttattaatg ataagaaata
     9241 tgttttgcga gcagcaacta gaataattta aacattacta aactgtctat attgttacta
     9301 ataattccct tcttatagtg cattttctta gaaaaaaaca atattacgat atcatgatat
     9361 taagatatcg taatattata caattgtgat tgatattata tatgtttttt tattttttta
     9421 tcatgtttaa tcttgaagga atcatattct ttttctaaag acaggagctt ttcataaaca
     9481 tcagacatgt ttaaactaga ggccaggctt aaatcataaa cga
//
