LOCUS       CP064286               15928 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  Enterococcus faecium strain Cairo plasmid pEFAEC_CAIRO_frag5.
ACCESSION   CP064286
VERSION     CP064286.1
DBLINK      BioProject: PRJNA672694
            BioSample: SAMN16574827
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15928)
  AUTHORS   Broddrick,J.T., Szubin,R., Norsigian,C.J., Monk,J.M., Palsson,B.O.
            and Parenteau,M.N.
  TITLE     High-quality genome-scale models from error-prone, long-read
            assemblies
  JOURNAL   Front Microbiol (2020) In press
REFERENCE   2  (bases 1 to 15928)
  AUTHORS   Broddrick,J.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2020) Exobiology Branch, NASA, Bldg N239 Rm 315,
            Moffett Field, CA 94035, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date          :: 20-APR-2019
            Assembly Method        :: Flye v. 2.6; Racon v. 1.4.7; Medaka v. 0.9
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 46x
            Sequencing Technology  :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="Cairo"
                     /isolation_source="clinical isolate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pEFAEC_CAIRO_frag5"
                     /geo_loc_name="Egypt: Cairo"
                     /collection_date="06-Jul-2018"
     ARG             10551..11578
                     /name="vanA"
                     /gene="vanA"
                     /gene_name="D-alanine--(R)-lactate ligase VanA"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="99.71"
                     /coverage="100.0"
     ARG             6041..6732
                     /name="vanR-A"
                     /gene="vanR-A"
                     /gene_name="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             9590..10554
                     /name="vanH-A"
                     /gene="vanH-A"
                     /gene_name="D-lactate dehydrogenase VanH-A"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             11588..12192
                     /name="vanX-A"
                     /gene="vanX-A"
                     /gene_name="D-Ala-D-Ala dipeptidase VanX-A"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             6714..7864
                     /name="vanS-A"
                     /gene="vanS-A"
                     /gene_name="vancomycin resistance histidine kinase VanS-A"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             13688..14169
                     /name="vanZ-A"
                     /gene="vanZ-A"
                     /gene_name="glycopeptide resistance protein VanZ-A"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             12624..13531
                     /name="vanY-A"
                     /gene="vanY-A"
                     /gene_name="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="99.67"
                     /coverage="100.0"
     ARG             9590..10557
                     /name="vanH gene in vanA cluster"
                     /gene="vanH gene in vanA cluster"
                     /gene_name="vanH; glycopeptide resistance gene cluster"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             10533..11581
                     /name="vanA"
                     /gene="vanA"
                     /gene_name="glycopeptide resistance gene cluster; Van
                     ligase"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="99.71"
                     /coverage="101.75"
     ARG             6041..6735
                     /name="vanR gene in vanA cluster"
                     /gene="vanR gene in vanA cluster"
                     /gene_name="glycopeptide resistance gene cluster; vanR"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             12624..13534
                     /name="vanY gene in vanA cluster"
                     /gene="vanY gene in vanA cluster"
                     /gene_name="vanY; glycopeptide resistance gene cluster"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="99.67"
                     /coverage="100.0"
     ARG             11588..12195
                     /name="vanX gene in vanA cluster"
                     /gene="vanX gene in vanA cluster"
                     /gene_name="vanX; glycopeptide resistance gene cluster"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="99.5"
                     /coverage="100.0"
     ARG             13688..14172
                     /name="vanZ gene in vanA cluster"
                     /gene="vanZ gene in vanA cluster"
                     /gene_name="vanZ; glycopeptide resistance gene cluster"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             6714..7868
                     /name="vanS gene in vanA cluster"
                     /gene="vanS gene in vanA cluster"
                     /gene_name="vanS; glycopeptide resistance gene cluster"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="100.0"
                     /coverage="94.79"
     Typing          complement(4027..4903)
                     /name="rep_cluster_1093"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.238"
                     /coverage="91.0"
                     /evalue="0.0"
ORIGIN
        1 gggcaaaatt tcccgcctaa aaattttctg cttttttagc caaacaggtt cgcgaatttt
       61 actaaactgg gaaacttgct ccctctggtc gctaaaaagg tgtgagggaa aaacaccctc
      121 acattgtacc tgcgcagtcg cctaaaggct cctttgctaa aaacgttgtt taaagagatt
      181 ttttagaggc tctgcgcgct cgtttaagcc gttttaagac agtttagtat ggataaccct
      241 tatcttgttc ttaaagcacc ttacaggcgg tcagggcaag ccctaacaac ccttaaagag
      301 cataaaacca tcctaaaatt cgatttaatc tgtcttatct atgttcttgt atattttata
      361 taccttgggt agtaaaacgt ctgaaaacgc aaataagagc gtatgaaaca aaacaataag
      421 caaccgattc agcagaattc cggaggagcc attttcgtac gttagcaaaa aagtgctgtt
      481 gctaaaagct gcttgcggtg cggagcgaac aaggtctcct agagcgagac gtgtacaaac
      541 aagcttacaa attcgtgaaa ggcaccggtt ttgcgccatt tccaagcaaa aaaaggtcta
      601 tcgagcaagc tcgatagacc gatcgcattt agccccccgc attctaacag ggggggaata
      661 atgtgaaaaa ttatggtagt aatgcttgat atataggcat ttcttttggt tgcacactta
      721 aaaaaaatca cgattttgac aactaaattt ttaaataaaa aagctttaca gtttaattca
      781 aatgtgtttt aatatatcta tcagtacttg ccacgcctct gatttaatat catgcgcatt
      841 atggtagggc ttttttttta tattttttta agaaagaaga ttcttatgaa gacatcaaat
      901 agaaaatata aagttaaaac agttaatcat agaattccac ctaaaaaggc attatgtgta
      961 attaataatt ccaaagtaat taatcaagag ttaaaaccat atttatccga gcaagaaatg
     1021 ataaaaaaaa taagtaaaga tattttttta acaaattccg atacaatttt attagaaaag
     1081 tttttaaaga aaaatagtta ttttcgattt agtatttatg ttaaattaat gaaaaatata
     1141 gataaagtta ctattctaga tgttattgaa acgtacaaat tagattcatt cattcgtaat
     1201 caattgcatt attttgtgaa tcaaatagaa attttttgga aaaaatctct ttcagataat
     1261 atgtgcgttt cttatgaaga aactagcata tttccgaaga atcagtgtta tttggataaa
     1321 aatatttatt cagatttgaa atgggctgaa gatataattg gtcattttaa tagttttttt
     1381 tattcaaatc aaagtcccac ttttaaacat catcatataa aaaaaatcat tgtttaccaa
     1441 tatgggcttt atttgaagaa ataacatttg gtagtttaac aacatttatt aatcaactta
     1501 atacagagta ttataataat tgggtcatga gctgttatga taatcctaaa tacaaaaaag
     1561 ctatacgaag ttgggcaaac gtaattagag cttatagaaa taagatagct catcattcaa
     1621 gaatatatgc atttaaagtt actgacgctc caacaatcat aaaaaatgat tccaaaagtt
     1681 attttcctga aaataactta aaagaaaccc aaaagttata tttgtatgga ggattatacg
     1741 ttataaaaca tctgttaata tatgaagata gcatgacaca aactttatgg aataaatttc
     1801 taatagattt agaaaaaaag atcgataaaa tcccttcctt agaaaaatcc aattatggtt
     1861 ttccggaaaa ttggagaaac aagttaaaaa taatgattgt ttaattgtta tttaaattgt
     1921 tagtagacat aatgtacata ctaccaagta gcccctggat caaccgatcc aggggaagac
     1981 tttgtgattt tcaattggtc tatctaaaac atctttttaa aagcttgatt cttcgctgtt
     2041 ggcttttttc gattctttgt gaatattcag tgattatttt tttactactt gttattttcc
     2101 gagggatttg ttaaaagttt aatagcgttt ctcagtaagg gacgtcgttt tttcattttc
     2161 ctatgtatgg actgctcgaa cgagcagcct ttttttattc cagggaacgt cctattcttt
     2221 gagtttccat gctattgaat aaccgggact tagggattag ggcagcgccc caaaatgaaa
     2281 aaagaaaaat ccgcttctgc gaattcttct tttttttcgc aagttggtac caacttgcaa
     2341 atcttgctgg aagaccgatc cctaaaaaga aatcgaggaa tttttttgtt tttcggtttc
     2401 tttttttatc tttttagaaa gtttgttggg tcaaaatttc cagaagtaaa ggtttgattg
     2461 gtttcttttt gacgattttt gggttgattt cgtcgggaag gattgagttg attttcaatt
     2521 ttttctttca cttcttgata tactttagaa tttgtaccga taagaacacc agtatctttt
     2581 agatataaat cagcaaattc tgccatgatt tctgtcatga tagaaatttc ttttcccatg
     2641 gcattgagtt ttgcttggtt actggttgtg gttgtattgg tatcaggttc ataactcata
     2701 cataaaaagc gaatggcttc tgaataattg ttaacgtctg gatttttttc gatgatggaa
     2761 tctaaaacgt caacgtgact gggataaagg gttacgtgtt tttgaacaga agtttttctc
     2821 atgttatatt cctccgataa gtgtttcaat gagttggttg tttgtgtgaa catagtcatt
     2881 tagtatgttc gtattttctg cttgaatggc aataaatttt tcgaataata aatcgaattg
     2941 atcttttttg taattcataa gttcgtggtc aaaatcgttt tcgattaaaa tttttaggta
     3001 ttgatttcga ctccattttt tgcccgtttt ggttgttagt tcctgacaac gtttatctat
     3061 tcttgtaata attttgggat tgatatttcg aactaaaatg tccatggaaa ttttcctttc
     3121 gtatcagtgt ataatttatg tatatcatac aaatatagta tttgtatagc tatttttcgc
     3181 caaaacacta tacatagcct agccacaatg gcaaaaaagt gatagcaaat ttttttctga
     3241 gaaacgtgat aggcacagct tatctagcac ctttcgtaag aaagtgatac cacgtttcct
     3301 tatgctcttt caaaattcag aaaaatcagc tgtaaaacga gtgcagtccg agtaagattc
     3361 aacgaaaaaa cggggattgg ggcgccacca aaatatcgca acatgggccc atgttgctct
     3421 gctcgcaaaa agaaaaagaa gaataaaacc agcaattttt attaatttaa attgctggtt
     3481 ttattgccag aattgccacc attttttctc cgtctttgat tgtttttcta tcgattctgt
     3541 ttcttcagaa agagtcgttg atttattttc cgaagattct tccgtataag tcaactgtag
     3601 ttgttcctgc agtttctcga tttgttggtt ggcttgcagg gtgagttgtt gttgttggtc
     3661 gagtaatttt tgcatttgtt ttatttgctc gtctttttct tgtagttgca tatctttttg
     3721 atttaactgt tgttctagga gagaaaggac ttctaattcg ggaacttggt tggcgttttt
     3781 gtggttttcg gttttagttt tttcgccaac tagttgttga tttgagtttt cgttttcgtt
     3841 aaaaaacatt gattttatag ccttttgtcc ggtttcatta atcacaaact tgtttccgat
     3901 tttcgaaaac caaagttttc gattttcgtc agaaactttt ttgttaattg ctgttttact
     3961 aactcctaat tcttcagcaa gttctttgat tgttttttct gtcataaaat ctaaaaaccc
     4021 ctcaatcttt gaataagttt tgaagattgt ctaataagtc tttttttagt tctgaaggaa
     4081 tatcggaatt tatctctgat ttttgtgcat tattttcttt attttgggta ttttgttctc
     4141 tttctaactt ttctaactgt tgtaaaagtt ccaattttcg cttttgttct ttgatttttc
     4201 ttgtaatgaa gtaggtgctt tttcagattg aacttgttgg ttagctagtt ctaatgcttt
     4261 gttttgttct tcttttgttg gggcatgttt gttaaagtcg tctgattttt ttatctcagg
     4321 tttaaatgta aattgatagc caatgacagg ctttccccgt ccttttccgt atctcttttg
     4381 gattgtcaga cctttgaaga ttgcggttag ttcttggcga ataggcttta taattttttt
     4441 atttatatca ctttctctat aacttttagg gatatcaagt aattcatgaa agtcttcttt
     4501 tgaaaaatag gcaattcctt tggttctgta ttgttttaat aatctaaaca tggttttgga
     4561 ataactgctt tctaattcat tgaattgttg gagagaatat cgaacccatt cttcaagatt
     4621 attaagtaag ggtaaggctt tctcatggac ttgtatttca gcaaaaggaa tatcagcttt
     4681 tccatcgatt ttaaattgat taaataaaac aaatcgttca cgttgcaaac cgtcagcact
     4741 tcttctacca aaacgtaaat ccattaattt gtcatacgtt gtttctaaat catctatgaa
     4801 tcgaacattt gcagttgctt tataattgct taaatctttt aattgttcga atgtaaattg
     4861 tactttttta tcgcctacgt cacgcattct agatactata gagggttctg ttgcaaagtt
     4921 ttaaataaag aataaaatcc cttacggtat ctatgattta agctgggatt cccaataata
     4981 ccttgatttc agtacagacc gaaaacccga agagagtgcc ttcttttcgg gttttcttat
     5041 ataatcctcg aatggcttcc atgcctttaa tcgtggtaga ggcagtgcgt aaacttcgat
     5101 agaatttatt gcgtctcttt actggacgat ggtcttgttc aatcaaatta ttcaggtatt
     5161 taatggtacg atgttctgtc ccttgataaa agccgtattc ttttagtttc ttaaaggcac
     5221 ttgtaataga gggggcttta tctgtgacta caaccttcgg ttcatcaaac tgcttcacta
     5281 accgcttaag aaaagcatag gctgcttgtg tgtcccgttt tttacgtaac caaatatcca
     5341 aggttaaacc atctgcatcg atggctcgat acaaataatg ccattttcct ttaattttga
     5401 tgtacgtttc atccattttc catgaataaa aggatttttt atttttcttt ttccaaattt
     5461 gatagagtag tttgccatat tcttgcaccc aacgataaat cgtcgtatga gaaacgttaa
     5521 tgccacgatc atataagatt tcttgaactt cacgatagct aaggttataa cgaagatagt
     5581 agcccacggc tacaataatc acatcctgct gaaattgctt tcctttaaaa tgattcatcg
     5641 tcattcctcc tgctatcttt ttctattatt ctaccttatt tgatagtaga tttaaaactt
     5701 tgcaacagaa ccaattatgc ggtaaagcta tataaagaag gaaatatgac tgtaaatcaa
     5761 atttgtgaaa ttactaatgt atctagggct tcattataca ggaaattatc agaagtgaat
     5821 aattagccat tctgtattcc gctaatgggc aatattttta aagaagaaaa ggaaactata
     5881 aaatattaac agcctcctag cgatgccgaa aagccctttg ataaaaaaag aatcatcatc
     5941 ttaagaaatt cttagtcatt tattatgtaa atgcttataa attcggccct ataatctgat
     6001 aaattattaa gggcaaactt atgtgaaagg gtgataacta tgagcgataa aatacttatt
     6061 gtggatgatg aacatgaaat tgccgatttg gttgaattat acttaaaaaa cgagaattat
     6121 acggttttca aatactatac cgccaaagaa gcattggaat gtatagacaa gtctgagatt
     6181 gaccttgcca tattggacat catgcttccc ggcacaagcg gccttactat ctgtcaaaaa
     6241 ataagggaca agcacaccta tccgattatc atgctgaccg ggaaagatac agaggtagat
     6301 aaaattacag ggttaacaat cggcgcggat gattatataa cgaagccctt tcgcccactg
     6361 gagttaattg ctcgggtaaa ggcccagttg cgccgataca aaaaattcag tggagtaaag
     6421 gagcagaacg aaaatgttat cgtccactcc ggccttgtca ttaatgttaa cacccatgag
     6481 tgttatctga acgagaagca gttatccctt actcccaccg agttttcaat actgcgaatc
     6541 ctctgtgaaa acaaggggaa tgtggttagc tccgagctgc tatttcatga gatatggggc
     6601 gacgaatatt tcagcaagag caacaacacc atcaccgtgc atatccggca tttgcgcgaa
     6661 aaaatgaacg acaccattga taatccgaaa tatataaaaa cggtatgggg ggttggttat
     6721 aaaattgaaa aataaaaaaa acgactattc caaactagaa cgaaaacttt acatgtatat
     6781 cgttgcaatt gttgtggtag caattgtatt cgtgttgtat attcgttcaa tgatccgagg
     6841 gaaacttggg gattggatct taagtatttt ggaaaacaaa tatgacttaa atcacctgga
     6901 cgcgatgaaa ttatatcaat attccatacg gaacaatata gatatcttta tttatgtggc
     6961 gattgtcatt agtattctta ttctatgtcg cgtcatgctt tcaaaattcg caaaatactt
     7021 tgacgagata aataccggca ttgatgtact tattcagaac gaagataaac aaattgagct
     7081 ttctgcggaa atggatgtta tggaacaaaa gctcaacaca ttaaaacgga ctctggaaaa
     7141 gcgagagcag gatgcaaagc tggccgaaca aagaaaaaat gacgttgtta tgtacttggc
     7201 gcacgatatt aaaacgcccc ttacatccat tatcggttat ttgagcctgc ttgacgaggc
     7261 tccagacatg ccggtagatc aaaaggcaaa gtatgtgcat atcacgttgg acaaagcgta
     7321 tcgactcgaa cagctaatcg acgagttttt tgagattaca cggtataacc tacaaacgat
     7381 aacgctaaca aaaacgcaca tagacctata ctatatgctg gtgcagatga ccgatgaatt
     7441 ttatcctcag ctttccgcac atggaaaaca ggcggttatt cacgcccccg aggatctgac
     7501 cgtgtccggc gaccctgata aactcgcgag agtctttaac aacattttga aaaacgccgc
     7561 tgcatacagt gaggataaca gcatcattga cattaccgcg ggcctctccg gggatgtggt
     7621 gtcaatcgaa ttcaagaaca ctggaagcat cccaaaagat aagctagctg ccatatttga
     7681 aaagttctat aggctggaca atgctcgttc ttccgatacg ggtggcgcgg gacttggatt
     7741 ggcgattgca aaagaaatta ttgttcagca tggagggcag atttacgcgg aaagcaatga
     7801 taactatacg acgtttaggg tagagcttcc agcgatgcca gacttggttg ataaaaggag
     7861 gtcctaagag atgtatataa ttttggctct ttgtcaaatg atgttggtaa agagaaatct
     7921 tagaataatt ggagatcaag tggatcagtt cctcttgatc ttttttagtt gaaaatcaat
     7981 gcgttgccat gacgaatcat gatgcgcaat ttgaaattat aaaagctacg aaagccatat
     8041 gcatttcgtt ttaacacttt gatatggttg ttcagacatt caagtgggcc atttgaataa
     8101 ctttgttgta aagcattttt gatttgtggc agaaatttct ggaacgttct caaggtcgtc
     8161 tgatagcctt cgggaagcat gatattattg gtaagtagtt cttcaaacga tacgtagtct
     8221 cgttccttta cagcgtaaag aaagtcttga tataactgat agcctacacg caaagcaggg
     8281 ctccctttca gtaagcgatc cacaatatcg gtctctgtta aatgagcttt gaagccaggt
     8341 cgccaaaggc gtcgagtata atcaagcttg cgttcatctt tttgaagaag tttccagtat
     8401 ctttttaatt gcttacctaa ttgatagtgt ttttgttctt tgctttttaa tagttggttc
     8461 gtttctttga ttcgatgatt gcgaaaggta cgaccaatat gttggacgat gtgaaaccgg
     8521 tcaataatca gctgtgcttt tggaaacagc tttttaacga gcgatacata aggagcgtac
     8581 atatcaatca cgatccattc gaccgcttca cgaatttctc gtgaaaaatg actaaaataa
     8641 cgttttaaaa aaggcagttg tcggttctcg acaatgtcca ataagcgctg tgtttgtcca
     8701 tccatcatga tgaaactcat agcacctgaa acaccccgaa ctgatttgaa ttcatcaaaa
     8761 caaagtacct taggtaacgg tgtccgaaaa ggattcaacg gacgataaaa tcgcttcata
     8821 atacggtaaa cagaagagac cgatagattt ttctgagcgg cgatctctgt cattgaggta
     8881 ttttttttca gacgagtcac gacagaccag cggactttct cactgatgaa acaatgtttt
     8941 ttcgcaacag aagtatctgc gacaaaggtc cgctgacaag ctttgcattt gaagcgttgc
     9001 tttttcaatt cgaggtaggt acggtattca gaaacatcgt tcattaacaa ccggacggtt
     9061 gttgtacccc aacgcacaat atctgaagca atctggtgtt tacagtggat gcattcttgg
     9121 gctgtataaa tcaagtttcc acgataaata tgacagattt gccctttgta tttttctttt
     9181 gtgagacaat tttcgttaaa agtaactgat aagtctagaa tatctaatgt ttctttgata
     9241 aggtgagtgt aagacatctt gattcctcct tttgggttgg ttcgccaaat taattctaaa
     9301 ggatttcaag gtgtcttttc tatatgtttt ggaaaataaa aaagatgctg gtgtcatcca
     9361 ccaacatcaa aaagtataga gccataattt tttaggaaaa tctcaaggtt atctttactt
     9421 tttcttagga aattaacaat ttaatattaa gaaacggctc gttcttacac ggtagactta
     9481 ataccgtaag aacgagccgt tttcgttctt cagagaaaga tttgacaaga ttaccattgg
     9541 catccccgtt ttatttggtg cctttcacag aaagggttgg tcttaattat gaataacatc
     9601 ggcattactg tttatggatg tgagcaggat gaggcagatg cattccatgc tctttcgcct
     9661 cgctttggcg ttatggcaac gataattaac gccaacgtgt cggaatccaa cgccaaatcc
     9721 gcgcctttca atcaatgtat cagtgtggga cataaatcag agatttccgc ctctattctt
     9781 cttgcgctga agagagccgg tgtgaaatat atttctaccc gaagcatcgg ctgcaatcat
     9841 atagatacaa ctgctgctaa gagaatgggc atcactgtcg acaatgtggc gtactcgccg
     9901 gatagcgttg ccgattatac tatgatgcta attcttatgg cagtacgcaa cgtaaaatcg
     9961 attgtgcgct ctgtggaaaa acatgatttc aggttggaca gcgaccgtgg caaggtactc
    10021 agcgacatga cagttggtgt ggtgggaacg ggccagatag gcaaagcggt tattgagcgg
    10081 ctgcgaggat ttggatgtaa agtgttggct tatagtcgca gccgaagtat agaggtaaac
    10141 tatgtaccgt ttgatgagtt gctgcaaaat agcgatatcg ttacgcttca tgtgccgctc
    10201 aatacggata cgcactatat tatcagccac gaacaaatac agagaatgaa gcaaggagca
    10261 tttcttatca atactgggcg cggtccactt gtagatacct atgagttggt taaagcatta
    10321 gaaaacggga aactgggcgg tgccgcattg gatgtattgg aaggagagga agagtttttc
    10381 tactctgatt gcacccaaaa accaattgat aatcaatttt tacttaaact tcaaagaatg
    10441 cctaacgtga taatcacacc gcatacggcc tattataccg agcaagcgtt gcgtgatacc
    10501 gttgaaaaaa ccattaaaaa ctgtttggat tttgaaagga gacaggagca tgaatagaat
    10561 aaaagttgca atactgtttg ggggttgctc agaggagcat gacgtatcgg taaaatctgc
    10621 aatagagata gccgctaaca ttaataaaga aaaatacgag ccgttataca ttggaattac
    10681 gaaatctggt gtatggaaaa tgtgcgaaaa accttgcgcg gaatgggaaa acgacaattg
    10741 ctattcagct gtactctcgc cggataaaaa aatgcacgga ttacttgtta aaaagaacca
    10801 tgaatatgaa atcaaccatg ttgatgtagc attttcagct ttgcatggca agtcaggtga
    10861 agatggatcc atacaaggtc tgtttgaatt gtccggtatc ccttttgtag gctgcgatat
    10921 tcaaagctca gcaatttgta tggacaaatc gttgacatac atcgttgcga aaaatgctgg
    10981 gatagctact cccgcctttt gggttattaa taaagatgat aggccggtgg cagctacgtt
    11041 tacctatcct gtttttgtta agccggcgcg ttcaggctca tccttcggtg tgaaaaaagt
    11101 caatagcgcg gacgaattgg actacgcaat tgaatcggca agacaatatg acagcaaaat
    11161 cttaattgag caggctgttt cgggctgtga ggtcggttgt gcggtattgg gaaacagtgc
    11221 cgcgttagct gttggcgagg tggaccaaat caggctgcag tacggaatct ttcgtattca
    11281 tcaggaagtc gagccggaaa aaggctctga aaacgcagtt ataaccgttc ccgcagacct
    11341 ttcagcagag gagcgaggac ggatacagga aacggcaaaa aaaatatata aagcgctcgg
    11401 ctgtagaggt ctagcccgtg tggatatgtt tttacaagat aacggccgca ttgtactgaa
    11461 cgaagtcaat actctgcccg gtttcacgtc atacagtcgt tatccccgta tgatggccgc
    11521 tgcaggtatt gcacttcccg aactgattga ccgcttgatc gtattagcgt taaaggggtg
    11581 ataagcatgg aaataggatt tactttttta gatgaaatag tacacggtgt tcgttgggac
    11641 gctaaatatg ccacttggga taatttcacc ggaaaaccgg ttgacggtta tgaagtaaat
    11701 cgcattgtag ggacatacga gttggctgaa tcgcttttga aggcaaaaga actggctgct
    11761 acccaagggt acggattgct tctatgggac ggttaccgtc ctaatcgtgc tgtaaactgt
    11821 tttatgcaat gggctgcaca gccggaaaat aacctgacaa aggaaagtta ttatcccaat
    11881 attgaccgaa ctgagatgat ttcaaaagga tacgtggctt caaaatcaag ccatagccgc
    11941 ggcagtgcca ttgatcttac gctttatcga ttagacacgg gtgagcttgt accaatgggg
    12001 agccgatttg attttatgga tgaacgctct catcatgcgg caaatggaat atcatgcaat
    12061 gaagcgcaaa atcgcagacg tttgcgctcc atcatggaaa acagtgggtt tgaagcatat
    12121 agcctcgaat ggtggcacta tgtattaaga gacgaaccat accccaatag ctattttgat
    12181 ttccccgtta aataaacttt taaccgttgc acggacaaac tatataagct aactctttcg
    12241 gcaggaaacc cgacgtatgt aactggttct tagggaattt atatatagta gatagtattg
    12301 aagatgtaag gcagagcgat attgcggtca ttatctgcgt gcgctgcggc aagatagcct
    12361 gataataaga ctgatcgcat agaggggtgg tatttcacac cgcccattgt caacaggcag
    12421 ttcagcctcg ttaaattcag catgggtatc acttatgaaa attcatctac attggtgata
    12481 atagtaaatc cagtagggcg aaataattga ctgtaattta cggggcaaaa cggcacaatc
    12541 tcaaacgaga ttgtgccgtt taaggggaag attctagaaa tatttcatac ttccaactat
    12601 atagttaagg aggagactga aaatgaagaa gttgtttttt ttattgttat tgttattctt
    12661 aatatactta ggttatgact acgttaatga agcactgttt tctcaggaaa aagtcgaatt
    12721 tcaaaattat gatcaaaatc ccaaagaaca tttagaaaat agtgggactt ctgaaaatac
    12781 ccaagagaaa acaattacag aagaacaggt ttatcaagga aatctgctat taatcaatag
    12841 taaatatcct gttcgccaag aaagtgtgaa gtcagatatc gtgaatttat ctaaacatga
    12901 cgaattaata aatggatacg ggttgcttga tagtaatatt tatatgtcaa aagaaatagc
    12961 acaaaaattt tcagagatgg tcaatgatgc tgtaaagggt ggcgttagtc attttattat
    13021 taatagtggc tatcgagact ttgatgagca aagtgtgctt taccaagaaa tgggggctga
    13081 gtatgcctta ccagcaggtt atagtgagca taattcaggt ttatcactag atgtaggatc
    13141 aagcttgacg aaaatggaac gagcccctga aggaaagtgg atagaagaaa atgcttggaa
    13201 atacgggttc attttacgtt atccagagga caaaacagag ttaacaggaa ttcaatatga
    13261 actatggcat attcgctatg ttggtttacc acatagtgcg attatgaaag aaaagaattt
    13321 cgttctcgag gaatatatgg attacctaaa agaagaaaaa accatttctg ttagtgtaaa
    13381 tggggaaaaa tatgagatct tttattatcc tgttactaaa aataccacca ttcatgtgcc
    13441 gactaatctt cgttatgaga tatcaggaaa caatatagac ggtgtaattg tgacagtgtt
    13501 tcccggatca acacatacta attcaaggag gtaaggatgg cggaatgaaa ccaacgaaat
    13561 taatgaacag cattattgta ctagcacttt tggggtaacg ttagcttttt aatttaaaac
    13621 ccacgttaac taggacattg ctatactaat gatacaactt aaacaaaaga attagaggaa
    13681 attatattgg gaaaaatatt atctagagga ttgctagctt tatatttagt gacactaatc
    13741 tggttagtgt tattcaaatt acaatacaat attttatcag tatttaatta tcatcaaaga
    13801 agtcttaact tgactccatt tactgctact gggaatttca gagagatgat agataatgtt
    13861 ataatcttta ttccatttgg cttgcttttg aatgtcaatt ttaaagaaat cggattttta
    13921 cctaagtttg cttttgtact ggttttaagt cttacttttg aaataattca atttatcttc
    13981 gctattggag cgacagacat aacagatgta attacaaata ctgttggagg ctttcttgga
    14041 ctgaaattat atggtttaag caataagcat atgaatcaaa aaaaattaga cagagttatt
    14101 atttttgtag gtatactttt gctcgtatta ttgctcgttt accgtaccca tttaagaata
    14161 aattacgtgt aagatgtcta aatcaagcaa tctgatcttt catacacata aagatattga
    14221 atgaattgga ttagatggaa aacgggatgt ggggaaactc gcccgtaggt gtgaagtgag
    14281 agggaaaacc ggtgataaag taaaaagctt acctaacact atagtaacaa agaaagccca
    14341 attatcaatt ttagtgctga ggaattggtc tctttaataa atttccttaa cgttgtaaat
    14401 ccgcattttc ctgacggtac ccccaaacta agatttcata gatagatttt ctgataactt
    14461 taaaaaacac gaatagcgga aaggatgtct tcattcgcta aagagtaata aactactttt
    14521 ccatcttgac gtgattttgc cattccatgt ttttttaaaa aacgtaaatg gtgtgacgtt
    14581 gtagcgacac tagcagtaag tatttctgct aaatcacaga cgcacatttc cttataggtt
    14641 tctaaagcat agaaaatttt aattctagaa aaatcgctga aacatttccc taacacaagt
    14701 aagtttgaaa aatctttttg atccaattta ttttttattc catttacttt ttctttatgt
    14761 acgacagtca cttggcaaac ttttttcatt catctcacct cacattcaaa cgattatttg
    14821 aatgaagtat acctatttga tcactctttt gtcaatctac cttaattcca atacactatt
    14881 ataagcaatt tcatgtattg aagtaaactt acttgcaagt gcgtttttga ttccaagtta
    14941 aaaacatatt attattctac ataatttcct aatcaaataa aactcctttg aaaattgttc
    15001 taaaggaatt ttattatttt gtatttctac taaaaatagc gtacaatgta attactaagt
    15061 aattacgaaa tgtaaaataa tggaggaaat aatatgatta caaaaacaag aaaacaagga
    15121 aattctatta tgttaacggt ccctaaagaa ttcgatgtgc ccaatggtgt tgaagtagaa
    15181 gctaagcttg tagagaatgg tatcctttat gagtttgtag agcctaaaaa agaatttttt
    15241 gattttagtg aagatatttt ggctgatatt ctttcagaag gttatagcaa acaagagatc
    15301 ttaaaagaat ttagaaatag aaaaaatgcg ttaacttctg catttaaatc tattgctgaa
    15361 gatactgttg caaattctaa accgatgaca aaagaggagt tggcagcaga aattggctta
    15421 tgagattcta ccttctaaac acgtaataaa gtatctaaaa aaattaaaag agaaaacatt
    15481 aaaagaacaa tttttaacga taatttatga tgagatagca gttcgtcctc attcgggaga
    15541 acaaaaaacg ggtgatttat caggaatttg ggctattgga ttcaagtatg caggtacgac
    15601 atatcgcgtt gcatatgaaa ttaaagataa tgcagtgata cctatcttac tatgtggaac
    15661 gcatgaaaat ttttatgaac aattaaaaaa gattaggtaa gaacccaatg tttcaacttg
    15721 agaggataga cgacacttta aaagataatt tttaacagct gagagttgta ataccctata
    15781 attttaaacc actaacctat atatttatga aaaatagaaa aaattaaaac gcattttaag
    15841 cctctttcaa attaaaagct tcatccttat gcacaaagag ggcttaaaat gcgttatttt
    15901 atattgtttt ttagtaataa taaaaaaa
//
