LOCUS       CP064284               10815 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  Enterococcus faecium strain Cairo plasmid pEFAEC_CAIRO_frag3.
ACCESSION   CP064284
VERSION     CP064284.1
DBLINK      BioProject: PRJNA672694
            BioSample: SAMN16574827
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10815)
  AUTHORS   Broddrick,J.T., Szubin,R., Norsigian,C.J., Monk,J.M., Palsson,B.O.
            and Parenteau,M.N.
  TITLE     High-quality genome-scale models from error-prone, long-read
            assemblies
  JOURNAL   Front Microbiol (2020) In press
REFERENCE   2  (bases 1 to 10815)
  AUTHORS   Broddrick,J.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2020) Exobiology Branch, NASA, Bldg N239 Rm 315,
            Moffett Field, CA 94035, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date          :: 20-APR-2019
            Assembly Method        :: Flye v. 2.6; Racon v. 1.4.7; Medaka v. 0.9
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 46x
            Sequencing Technology  :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="Cairo"
                     /isolation_source="clinical isolate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pEFAEC_CAIRO_frag3"
                     /geo_loc_name="Egypt: Cairo"
                     /collection_date="06-Jul-2018"
ORIGIN
        1 aaaaagtaat catttactgg ctcttttgaa taatccatca taaataacac tccattcttt
       61 cacattatac aaacatatgt tcgatattgt aaagaagaaa tagctaaata cataaaaaaa
      121 gaagcatagg tatgctatca ccctgcttct tcttttgatt tgtatgatca atttattcta
      181 tcactttact aattaatttg aaaatctaat aaaaactctc actctaaaag agtgagagac
      241 tgcaaattag agtacggact tttttggagt tccgttcttt aatattaaca aatgaataaa
      301 atccttacaa catttgagaa taagttatca gtagacactt acaaaaaatt ctcatactat
      361 ttcctccatc attagttttt gtctatataa ggtttaccct aaatagacac tggtataaca
      421 ttgaaaaata tggcttcctc gtgtatgatt agggtgtatt ttaaatagtt gcagaaagta
      481 ggtaaaagtt ttgagagttg catacatacg tgtctcatcg atcgatcaaa atgaacaacg
      541 ccagattgaa gaaatgaaaa aattcggtgc agagagaatt ttcatcgaga aacaatctgg
      601 cgcaacgatt actcatcgac ctgttttcca agaagcatta gattacgtcc gagatcaaga
      661 tatttttatt gtggaagcta ttgatcgact tggaagaaat tatgacgaga tcatcgcttc
      721 agtcaattac ttgaagaaga aaaatgtgaa gctgatcatt actagtttac cgatcatggc
      781 tgaagcaatt ggcaatcctc tcctggataa attcattaag gatctgatca tccaaatttt
      841 ggccatgatc gctgaacaag aacggactga atcgaaacgt cgccaggcac aaggaattaa
      901 gatcgcaaaa gcaaacggcg tctacaaagg gcggcccaaa ttatacagtg cagacgcaaa
      961 agatccccaa cgtcgccttg tttacaaaag catcgttgaa gatcttaaaa atggtgttgc
     1021 gattgctaag attgctaaag actacaatgt tacgagacaa accgtgtata gaataaaaaa
     1081 agacagcatg gtcaatgaca aatagttgat catgctgctt ttattatctt ctcatcttta
     1141 atttcctaca tataattact atctataaaa ttaagtctta ctcagaacag ataaagcata
     1201 aaattccaac aaaccatttt ttacttaact aaataaagag tctgttaata taatcttcac
     1261 ttatttacgt taatccccta ctgtatttgt tggttcctta tctatatttt ctacaaaatc
     1321 cttattatct tgtgatttag cttcgtataa ttctttgtat gtcgacacat tggttatact
     1381 aattcctata gctagttctt gagactctac ttttctctct agctcctttg acaaagtgat
     1441 ttgagattta tttggttccg ttggagtcaa ttctatagat tcttcataaa aatctctata
     1501 cataaagcta tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca
     1561 ttcaaaacat tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg
     1621 tttttctatg aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta
     1681 ccctttaaac atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat
     1741 tagagttaaa tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt
     1801 aattatcgaa ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat
     1861 aagagaaaag gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga
     1921 agttatatct ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat
     1981 ttctatccaa ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc
     2041 aagcatcgct acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc
     2101 aatattgcta aaatcagcta atttttcttt agaataaaaa aaaacattta tatcagaatc
     2161 aataaattct atttttttta ttactatatc gtcatcatta tcttgaccat ttatgtttat
     2221 tgttccctga gtggggagta cttctttcaa attcaagtta aatttatatt ccattacttc
     2281 tcctccaata tataaaaatt acttaactgt atccaaattt aatatgtaaa taaatcatta
     2341 atttttagct aaattctatg agaagtttat ttttgttgtt tttgctaatt aactaaaact
     2401 agtacaaaaa atttaaaata gattattaaa cctattgctt attctattca gtcatatata
     2461 taagcgtcat cctttagtaa ctaacatccc acactacaat agtattataa catatctatt
     2521 caacttcaaa ttgttgaaat ttaagccatt tatatttact tttcctcaac tttagaaatg
     2581 attttactaa ccttatatcc gtaatacgcg taacaaacac attcattaat aacaatcaac
     2641 actaagctat attagctttt taaatgacta gggtaaataa aataaggatc aaaaactttt
     2701 aagtttctga ccctttcctt acttccgatt ggttaatagt gatcttttgc agaataaata
     2761 aatatcgttt cattttcaac tctatatatc agtctatgtt catctgtaat tcttctggac
     2821 cattttccag ataaatcatg ctttaatggc tcaggttttc ctaatccagc aaagggggaa
     2881 cgatcgatat cttttattaa cttgttaatc ttttttatat tgcttttgtt tccttgctca
     2941 tgccaataaa gataatcatc ccaagcatca tcagaccaag ccttaatcat cagattcaac
     3001 ctcgattaag tcatgtgttt taaatgcacc tttggagaat tgttcatctc ctcgacgaat
     3061 tttttccatg acgtaattat tagaaagtgt tctcaacgtt tcttgcatag aatcataatc
     3121 tctttttgat aatacaacaa ctgtatcttc tacatcttta cttgttacaa taagtgtttc
     3181 agcatcctca ttaacttgtt tcatataact acgtaaattt tggcggaaat ttgaataagc
     3241 tactgcttcc attcctttca ccccttattt atttctataa aacaattgta cattatattg
     3301 tacaattaag caaatgatta atattctaac ttcttataat taaaagtgct atcactttta
     3361 gtttttagga taaaaagtga cgtcactttt tgagtgctgg tacctcaaat cctgcttttt
     3421 cttgggcaat caataaatct aacgcaatac acataatcct ggattcactt ttgccgtact
     3481 tctgctttag ttgctgcaat tctttttgtc tcttttcatc caacgtaacg ggtaaccgtt
     3541 tatttcctat tgcaattggc attagtactc tctcctttca tcgaaataac cctatgaaaa
     3601 atatggctgc aatcatcttg aatagtagac tcacaagggc taatacaaag cgccaaaaga
     3661 tcaaacaaaa taccagtatt gccaaaaata ctacgaagtt taaatgcaca gctgcagcat
     3721 ggcgcataag tatttcgcca acaaatagga ctaacggttc tgttgcaaag ttttaaatct
     3781 actatcaaat aaggtagaat aatagaaaaa gatagcagga ggaatgacga tgaatcattt
     3841 taaaggaaag caatttcagc aggatgtgat tattgtagcc gtgggctact atcttcgtta
     3901 taaccttagc tatcgtgaag ttcaagaaat cttatatgat cgtggcatta acgtttctca
     3961 tacgacgatt tatcgttggg tgcaagaata tggcaaacta ctctatcaaa tttggaaaaa
     4021 gaaaaataaa aaatcctttt attcatggaa aatggatgaa acgtacatca aaattaaagg
     4081 aaaatggcat tatttgtatc gagccatcgg tgcagatggt ttaaccttgg atatttggtt
     4141 acgtaaaaaa cgggacacac aagcagccta tgcttttctt aagcggttag tgaagcagtt
     4201 tgatgaaccg aaggttgtag tcacagataa agccccctct attacaagtg cctttaagaa
     4261 actaaaagaa tacggctttt atcaagggac agaacatcgt accattaaat acctgaataa
     4321 tttgattgaa caagaccatc gtccagtaaa gagacgcaat aaattctatc gaagtttacg
     4381 cactgcctct accacgatta aaggcatgga agccattcga ggattatata agaaaacccg
     4441 aaaagaaggc actctcttcg ggttttcggt ctgtactgaa atcaaggtat tattgggaat
     4501 cccagcttaa atcatagata ccgtaaggga ttttattctt tatttaaaac tttgcaacag
     4561 aaccaaagtt ttaaatctac tatcaaataa ggtagaataa tagaaaaaga tagcaggagg
     4621 aatgacgatg aatcatttta aaggaaagca atttcagcag gatgtgatta ttgtagccgt
     4681 gggctactat cttcgttata accttagcta tcgtgaagtt caagaaatct tatatgatcg
     4741 tggcattaac gtttctcata cgacgattta tcgttgggtg caagaatatg gcaaactact
     4801 ctatcaaatt tggaaaaaga aaaataaaaa atccttttat tcatggaaaa tggatgaaac
     4861 gtacatcaaa attaaaggaa aatggcatta tttgtatcga gccatcgatg cagatggttt
     4921 aaccttggat atttggttac gtaaaaaacg ggacacacaa gcagcctatg cttttcttaa
     4981 gcggttagtg aagcagtttg atgaaccgaa ggttgtagtc acagataaag ccccctctat
     5041 tacaagtgcc tttaagaaac taaaagaata cggcttttat caagggacag aacatcgtac
     5101 cattaaatac ctgaataatt tgattgaaca agaccatcgt ccagtaaaga gacgcaataa
     5161 attctatcga agtttacgca ctgcctctac cacgattaaa ggcatggaag ccattcgagg
     5221 attatataag aaaacccgaa aagaaggcac tctcttcggg ttttcggtct gtactgaaat
     5281 caaggtatta ttgggaatcc cagcttaaat catagatacc gtaagggatt ttattcttta
     5341 tttaaaactt tgcaacagaa cccttcttct tcttcaaagg tatccacgta acggggaatg
     5401 tttaagttgt aatcattctc cacaatttca tcaaattctg cgacataagc atacttgtct
     5461 ttaattgtac gggttttata ggcctctaaa atagcgtcaa tatggtaatc tcgtagcgta
     5521 ttttgcgttt ttattttttc aaaatcttgt gacgcatcaa taaagagcac atcttttttc
     5581 gcacggtctt tcttcaagat aattacggtc gttggaatgg acgtattaaa gaagatgtta
     5641 gcaggtaagc caatgacggt gtcaatggca ccattttcta acaaggcttt gcgtatcttc
     5701 ccttctgcgc cccctctaaa gagcacccca tgtggcaaaa caatggccat gacgcccgta
     5761 tctttcaaat gatagtagcc atgtaataag aaagcaaagt ccgcttttga tttaggggct
     5821 aatacgccat aggcagagaa gcgtgggtca tctaagaagc ctttatcagc cgaccatttt
     5881 gcagaatacg gtgggttcat gagcacccca tcgaagttcg ttggctcttc tgttggccag
     5941 tcttgatcca aggtatccgc attatgcaag tgttggttgg caatgggaac cccatgcaaa
     6001 atcatgttca tacgcgccaa attataggta gacgtattca actcttgtcc gaaataagaa
     6061 atggttcccg gttgatggga atatttttta gcgttcaaaa gtaacgagcc cgaacccatg
     6121 gtcgcgtcgt aaacagaaaa gcctttttta tcttcttttc cttgtaaaac aatttgtgtc
     6181 atcaattttg cgaccggttg tggggtatag aattcaccgg cttttttacc ggattcagat
     6241 gcgaattggc caattaagta ctcataggca tcgcctaaga catcgccagc atgccccgcc
     6301 atgtttaacc cttctaattc tttcataaca tctgcgatgg ttttattttg tttttgtggg
     6361 gttgacccca gttttttaga atacagatca atgtcttcaa ataagttctc aaagttttcg
     6421 ctggattgtt caatgtcacg gaaaccttgt accaggtctt ctaattgaaa agatccatca
     6481 tgaatggctg ccacttgcgc ggtaaaggtc aatgcgggtg cgatggtata tgaaaactca
     6541 tcttttagtt catacaagag atcatccttt atatctgcat ctttaaatgc ttcaacgtaa
     6601 aggtcttgtg ctttatttag gtcgtccgtt tcttcctcca atagaacgga gacataccgc
     6661 agcatgttgt cggataagta tttataaaag accaagccta gtaaatagct cttgtattca
     6721 ttagcgtcca tttttgaacg caggatatct gcggaattcc acagtgcctg atataagctt
     6781 tttgaatttt tttcgatttg ttctgtcatt ggttattttc ctcattcgtg ttgtattttt
     6841 tataaatcag ttttttaaaa aattagttac tatttgaact tgttttttca ccatatgtat
     6901 tttaatgaaa tggataagct acatttgctt agacaattca tgtcttactt aattcttatt
     6961 ataacaaacc tatttcacct ttttcgatac attaaataat aagtaactta aaataaggtt
     7021 cgaataacct tgttaaatag tggttttctt atcttatagt atagaaatca agagattact
     7081 tccgatataa cattttttta aaaaaattag attgtaatgt tttttatatt cattctaaat
     7141 tttcacttaa aaaggataat cattaaaatc atcaaattca gtaatatata ttttcttact
     7201 cattttaata tatttctctt cgtctttaat ggtcccttca taaaaagatt tcttagcatc
     7261 ttcatttagc cagtctacag aaaacatctg attagagaca ggatctttga ttttagatac
     7321 aaattcggat gataaaacac tatcaaacat ttctccaaaa atggtggtca atctttgaaa
     7381 ccatatcatc ataaattcag tatttggctt taaagcatat ttgtttataa tcatttcaac
     7441 tatttctcta gccttactct cattttcgaa atttaataac tgccctataa ttaagacaca
     7501 ctgcggcaaa gaacccgggt tatttatcat gatatctacc aggataccaa taagtggtaa
     7561 aagatcgttc ggctttgaag tgattttaat aatgcgttct ttattaaact cagaaagtgc
     7621 ttttttcaat tggcctgaat taggatgctt atctgctaac ttttttattt ccaataaatg
     7681 tttttgtaaa tttatactat aacgaatttc tctatcacca tctaagtttg tggcattcat
     7741 tcttatactt gccttaattt cttcccaata gagcttatct tttttgatag aatctatgat
     7801 aatatcatct gataattttg ttttactaga atttaactta aaatttaaat catataagac
     7861 ttctgataac actttcataa tttgttccaa atcattacga ttatttgaaa atattctata
     7921 atcatctcta tatctgagta ctttgtaatc ttttaaattt tctaattttt ttaattcttt
     7981 cgataatctc aaatcaacat accctagaac gatctctgct acaaaatcca taagaacgga
     8041 accctgaggg ataccatttg tttgattgtg ttgcattcca gatatttttt tatctatttt
     8101 atcgccaagt gagcccgaac acttattttc atcattttta actttttctt taccatacat
     8161 ggcccatgtt atagagtgag tataaattga tggataacaa tcagcgatgt cagtaaacat
     8221 gcaatattgg tatttaatat tctgagctaa gcttttttgt tcaaaatatt cccaccagtt
     8281 tagtattgtt tcaccaagat ctgtcctttt tgaacctttg gaagcttcaa caggtataga
     8341 ataacagtta atatttttat cttttttaaa ttcactaaac ctctttttta actcctccca
     8401 atggtcttct ttagtgagac aattaaccaa atctacatat agaataggat gtagtagaac
     8461 gagaggacgc catgaatagt ttccatcttt attgataata agcgtatagt ttaagtcatc
     8521 acgttcttta tacattcgtg tatctacaat atcttttata tctttcggtt ggtttttttt
     8581 tgaagtatta tttgtcaaat taagcgagac aaaactttcc atttacgtaa ctcaaaaaaa
     8641 cttctaatgg tgtttggtaa tttagtgatt ttctagggat atggtttctt ttggacgcaa
     8701 cggatgagat gaatccttga ttgacttggt tgaagtccat ttcttttggt agtccatctt
     8761 ttcggaggag tccgtttgaa tgttcattta atccccgttg ggaaggcgtt cctgggtctg
     8821 cgaaataaat atcaatatca ttcgtattac taatactttt ccaattagag aattcttttc
     8881 cacaatcaaa ggtaattgat ttgaaaagat ttttgggtac ggattgaagc cattcattaa
     8941 tcgaattttc aatatcaact gccttacggc cttctggttt caagacgata atggcttttg
     9001 aaagtcgctc aacgagtgtg atgacggcac ttttatggtg gatgccgaca atcgtatcac
     9061 cctctaaatg tccaaattct tcttcgaaaa cgacataatc ttttttacgt tcagaaatat
     9121 ttcttttgaa tgcctgtttt ccacgttttt ccttataacc atttggtttt cgttttcctt
     9181 gcatcggtaa atgtaaaaca ttgaattctc ccgtcttaaa ccggcgatag agggtactta
     9241 ttgaacaaga gaaggttcgc tctgcacggc caataatgac gtctggggtc caaccttcag
     9301 ttgatctttt ttcaatgtat tctttttcct cagcaggaaa aatgatttcg gttctcccac
     9361 aacgtcgctt attttctttg tattgttcaa agtattcaag tgctgttcca ccacatttga
     9421 gaaagttata gacattgtaa attgtttggc gtccacgttt aagctgcttc gcaacgatag
     9481 caaccggagt ttcttgatga aaatatgctt ctatcattac aagctcgttt ggtgtaagat
     9541 gggtataagt catttatgtt cactctcctt gtatgcttta gcgggtatta caatttgagt
     9601 gtaacataaa tggctttttt atttgtctcg cttaattata caaacggcga agttaaaact
     9661 tttttagcag attttaaagt ttcgtcaaaa ctaatatatt ctggtagctg agcattcaaa
     9721 tagctttcag acttcagaag aaaattacga gcatttttat agtccatctc taataaagtt
     9781 attctttttt tatccaatac attcagctca attccttata atattctatt ccttagtatc
     9841 tcaacattat ttactttata aaacgaataa attttgtagt tctaattcaa atattgcatg
     9901 ttatatctaa tatgggagca ttttataatt ttcattgtga taaactcgtt attaacttta
     9961 ggatacacta gttaggatta aattaggtat ctagaaattt acttaagatt tttttgttta
    10021 tttcgtatac acactcgtat caaaaacaat cgattatgag acaaaatgaa atgacctctt
    10081 ttattaaata aaagaggtca tattttgtat cataacttgt tgcattaact atgtatctat
    10141 aactcccttt tatgatacaa tcaagccaga attaaagtac gaaaggaaga aaagcatgag
    10201 tggatacatt tatggttatg cccgagtgag tacacgttca caagaactaa atcgtcaatt
    10261 agacttactc aatgaacaaa attgtaatga aatcctaact gagaaaatga cgggaaccaa
    10321 agcggttcgt ccccaactaa atcgtttaaa agataaaatt cgtccgggag atacggtaat
    10381 tgtagaaagt ttttctcgat tgggtcgtag tacaaaggat ttgattgacc ttgttaccta
    10441 ttttgaagaa caccatgtta agctggtgag cttgaaagaa aacttcgata ccagcacgcc
    10501 tcacggacgc ttaatgatga ctgtcttcca agcatttagt cagttcgaac gtgatttaat
    10561 tgttgaacgt accaaagaag gtttgaaaag tgcacgtgcg agaggtcgta aaggtggccg
    10621 tccacgagtg aaccagaaag atgttgatcg agcagtaaaa ttgtataaaa gtcaggttta
    10681 tagcgtaaaa gaaattacgg aaatgactgg tattagcaag gcaacactct accgctactt
    10741 aaaagacaat agggaataat ctattttaat ctttaggaat ctataatatt tatgggagaa
    10801 ttagagtgat ttttt
//
