LOCUS       CP064283                4895 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  Enterococcus faecium strain Cairo plasmid pEFAEC_CAIRO_frag2.
ACCESSION   CP064283
VERSION     CP064283.1
DBLINK      BioProject: PRJNA672694
            BioSample: SAMN16574827
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Broddrick,J.T., Szubin,R., Norsigian,C.J., Monk,J.M., Palsson,B.O.
            and Parenteau,M.N.
  TITLE     High-quality genome-scale models from error-prone, long-read
            assemblies
  JOURNAL   Front Microbiol (2020) In press
REFERENCE   2  (bases 1 to 4895)
  AUTHORS   Broddrick,J.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2020) Exobiology Branch, NASA, Bldg N239 Rm 315,
            Moffett Field, CA 94035, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date          :: 20-APR-2019
            Assembly Method        :: Flye v. 2.6; Racon v. 1.4.7; Medaka v. 0.9
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 46x
            Sequencing Technology  :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="Cairo"
                     /isolation_source="clinical isolate"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pEFAEC_CAIRO_frag2"
                     /geo_loc_name="Egypt: Cairo"
                     /collection_date="06-Jul-2018"
     ARG             complement(4349..4887)
                     /name="sat4"
                     /gene="sat4"
                     /gene_name="streptothricin N-acetyltransferase Sat4"
                     /tool="amrfinderplus"
                     /drug_class="STREPTOTHRICIN"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(3462..4252)
                     /name="aph(3')-IIIa"
                     /gene="aph(3')-IIIa"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(4346..4893)
                     /name="SAT-4"
                     /gene="SAT-4"
                     /gene_name="streptothricin acetyltransferase (SAT)"
                     /tool="rgi"
                     /drug_class="nucleoside antibiotic"
                     /identity="98.89"
                     /coverage="101.11"
ORIGIN
        1 tttataatct ctttttaaac ctctttttaa acctctttac cgtcggctgt atgcttactc
       61 tcccaaggtg tgcagaatct tttgggggtc ttttttgtct gtttttaggg gtctttttgt
      121 ctgtttttag gggtcttttt gtctgttttt gggggtcttt ttgtctgttt ttaggggtct
      181 ttaattaggg gcttgacccc tattagaaag ctctttataa ttttgttaag tagttaatct
      241 tgaaggagga ttttatggca aatgaaattg tgaaatacca ccatgaatta aatacgattc
      301 ctttacgcaa atttacgtca gtagaaatga atttattctt ctctataggg ttctgttgca
      361 aagttttaaa tctactatca aataaggtag aataatagaa aaagatagca ggaggaatga
      421 cgatgaatca ttttaaagga aagcaatttc agcaggatgt gattattgta gccgtgggct
      481 actatcttcg ttataacctt agctatcgtg aagttcaaga aatcttatat gatcgtggca
      541 ttaacgtttc tcatacgacg atttatcgtt gggtgcaaga atatggcaaa ctactctatc
      601 aaatttggaa aaagaaaaat aaaaaatcct tttattcatg gaaaatggat gaaacgtaca
      661 tcaaaattaa aggaaaatgg cattatttgt atcgagccat cgatgcagat ggtttaacct
      721 tggatatttg gttacgtaaa aaacgggaca cacaagcagc ctatgctttt cttaagcggt
      781 tagtgaagca gtttgatgaa ccgaaggttg tagtcacaga taaagccccc tctattacaa
      841 gtgcctttaa gaaactaaaa gaatacggct tttatcaagg gacagaacat cgtaccatta
      901 aatacctgaa taatttgatt gaacaagacc atcgtccagt aaagagacgc aataaattct
      961 atcgaagttt acgcactgcc tctaccacga ttaaaggcat ggaagccatt cgaggattat
     1021 ataagaaaac ccgaaaagaa ggcactctct tcgggttttc ggtctgtact gaaatcaagg
     1081 tattattggg aatcccagct taaatcatag ataccgtaag ggattttatt ctttatttaa
     1141 aactttgcaa cagaaccggc tctatcattc tgctttccgg ttcttgccct cctgatgcag
     1201 gatattgaat tgatattacc cttttattgc agattgcagt ctgaattgct gcaataacgt
     1261 tatccgccaa tgtatttttc tgtccagatg tgctgtacac gttaatactg ttctccaact
     1321 cctgtgcata gtttttttca ccatgcttca aaatagattt gattttatac atagctgaat
     1381 caaaatcctt tataaatgat tcatctccat ggcaatttaa aaatttccct gctgtaatca
     1441 atgcacccac ttccgccgct gtaaacatta ccggcggaag taggaagccc tctacaagaa
     1501 aatatccttt tccggcctca gatccaattg gtattccggc ttcttcaagt gtccggatat
     1561 ctctgtaaac tgtccttaag cttatattaa atcgctgtgc aatttctttg gcaggaatta
     1621 ttttttttga ttgcagctga ataagaatag aggttactct gttaatcctg ttcaaatttt
     1681 attcctcctc tgtgacttcc ataaaaagta tatcctatgc gttcttaagc tagctgctgt
     1741 tagtgcttct cattgcaccc tggttataaa agccatactc acaaacctct tcaccaaaac
     1801 tctttacatc tctaatatag aactctatct agcgaacttt tagaaaagat ataaaacatc
     1861 agagtatgga cagttgcgga tgtacttcag aaaagaggct ctatactttt tgatgttggt
     1921 ggatgacacc agcatctttt ttattttcca aaacatatag aaaagacacc ttgaaatcct
     1981 ttagaattaa tttggcgaac caacccaaaa ggaggaatca agatgtctta cactcacctt
     2041 atcaaagaaa cattagatat tctagactta tcagttactt ttaacgaaaa ttgtctcaca
     2101 aaagaaaaat acaaagggca aatctgtcat atttatcgtg gaaacttgat ttatacagcc
     2161 caagaatgca tccactgtaa acaccagatt gcttcagata ttgtgcgttg gggtacaaca
     2221 accgtccggt tgttaatgaa cgatgtttct gaataccgta cctacctcga attgaaaaag
     2281 caacgcttca aatgcaaagc ttgtcagcgg acctttgtcg cagatacttc tgttgcgaaa
     2341 aaacattgtt tcatcagtga gaaagtccgc tggtctgtcg tgactcgtct gaaaaaaaat
     2401 acctcaatga cagagatcgc cgctcagaaa aatctatcgg tctcttctgt ttaccgtatt
     2461 atgaagcgat tttatcgtcc gttgaatcct tttcggacac cgttacctaa ggtactttgt
     2521 tttgatgaat tcaaatcagt tcggggtgtt tcaggtgcta tgagtttcat catgatggat
     2581 ggacaaacac agcgcttatt ggacattgtc gagaaccgac aactgccttt tttaaaacgt
     2641 tattttagtc atttttcacg agaaattcgt gaagcggtcg aatggatcgt gattgatatg
     2701 tacgctcctt atgtatcgct cgttaaaaag ctgtttccaa aagcacagct gattattgac
     2761 cggtttcaca tcgtccaaca tattggtcgt acctttcgca atcatcgaat caaagaaacg
     2821 aaccaactat taaaaagcaa agaacaaaaa cactatcaat taggtaagca attaaaaaga
     2881 tactggaaac ttcttcaaaa agatgaacgc aagcttgatt atactcgacg cctttggcga
     2941 cctggcttca aagctcattt aacagagacc gatattgtgg atcgcttact gaaagggagc
     3001 cctgctttgc gtgtaggcta tcagttatat caagactttc tttacgctgt aaaggaacga
     3061 gactacgtat cgtttgaaga actacttacc aataatatca tgcttcccga aggctatcag
     3121 acgaccttga gaacgttcca gaaatttctg ccacaaatca aaaatgcttt acaacaaagt
     3181 tattcaaatg gcccacttga atgtctgaac aaccatatca aagtgttaaa acgaaatgca
     3241 tatggctttc gtagctttta taatttcaaa ttgcgcatca tgattcgtca tggcaacgca
     3301 ttgattttca actaaaaaag atcaagagga actgatccac ttgatctcca attattctaa
     3361 gatttctctt taccaacatc atttgacaaa gagccagaaa agattagatg tctaaaaagc
     3421 ttgtagttaa agctttttag acatctaaat ctaggtacta aaacaattca tccagtaaaa
     3481 tataatattt tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga
     3541 catactgttc ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc
     3601 acttgtccgc cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca
     3661 caaagatgtt gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt
     3721 ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt
     3781 tttcgcaatc cacatcggcc agatcgttat tcagtaagta atccaattcg gctaatcggc
     3841 tgtctaagct attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc
     3901 actccgcata cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc
     3961 aaaggacgcc atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt
     4021 gcaggacctt tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt
     4081 ccacatcata ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat
     4141 tttctcccac cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc
     4201 ggtatttttc gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt
     4261 tccttcctct tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa
     4321 ctccaattca ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa
     4381 gttgttttca aagttggcgt ataacatagt atcgacggag ccgattttga aaccacaatt
     4441 atgatagaat ttacaagcta taaggttatt gtcctgggtt tcaagcatta gtccatgcaa
     4501 gtttttatgc tttgcccatt ctatagatat attgataagc gcgctgccta tgccttgccc
     4561 cctgaaatcc ttacatacgg cgatatcttc tatataagcg taccggttcc aattttttcg
     4621 cagtttaact tttccgacgc atttatcgtc ttggtagtaa agatatatta tcttatcagt
     4681 attgtcaata tattcaaggc aatctgcctc ctcatcctct tcatcctctt cgtcttggta
     4741 gctttttaaa tatggcgctt catagagtaa ttctgtaaag gtccaattct cgttttcata
     4801 cctcggtata atcttaccta tcacctcaaa tggttcgctg ggtttatcga tatctttcag
     4861 gtgccctgct ttcatttctg taatcactgt tcccg
//
