LOCUS       CP063744                6079 bp    DNA     circular BCT 15-OCT-2021
DEFINITION  Escherichia coli strain 18SC04V02-EC plasmid pVPS18EC0466-1,
            complete sequence.
ACCESSION   CP063744
VERSION     CP063744.1
DBLINK      BioProject: PRJNA292663
            BioSample: SAMN16533949
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6079)
  AUTHORS   Tate,H., Li,C., Nyirabahizi,E., Tyson,G.H., Zhao,S.,
            Rice-Trujillo,C., Jones,S.B., Ayers,S., M'ikanatha,N.M., Hanna,S.,
            Ruesch,L., Cavanaugh,M.E., Laksanalamai,P., Mingle,L.,
            Matzinger,S.R. and McDermott,P.F.
  TITLE     A National Antimicrobial Resistance Monitoring System Survey of
            Antimicrobial-Resistant Foodborne Bacteria Isolated from Retail Veal
            in the United States
  JOURNAL   J Food Prot 84 (10), 1749-1759 (2021)
   PUBMED   34015113
REFERENCE   2  (bases 1 to 6079)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2020) CVM/OR, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JUN-2020
            Assembly Method                   :: HGAP v. 4
            Assembly Name                     :: Microbial Assembly
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 654x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/27/2020 12:55:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.13
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,567
            Genes (coding)                    :: 4,418
            CDSs (with protein)               :: 4,418
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 53 of 149
            Pseudo Genes (incomplete)         :: 86 of 149
            Pseudo Genes (internal stop)      :: 43 of 149
            Pseudo Genes (multiple problems)  :: 30 of 149
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="18SC04V02-EC"
                     /isolation_source="Veal"
                     /db_xref="taxon:562"
                     /plasmid="pVPS18EC0466-1"
                     /geo_loc_name="USA:SC"
                     /collection_date="2018"
                     /collected_by="CVM"
     gene            complement(join(4552..6079,1..200))
                     /locus_tag="IO770_23390"
     CDS             complement(join(4552..6079,1..200))
                     /locus_tag="IO770_23390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin"
                     /protein_id="QOX49741.1"
                     /translation="MSGGDGRDHNSGAHNTGGNINGGPTGLGGNGGASDGSGWSSENNP
                     WGGGSGSGVHWGGGSGHGNGGGNGNSGGGSNSSVAAVAFGFPALAAPGAGTLGIAVSGE
                     ALSAAIADIFAALKGPFKFSAWGIALYGILPSEIAKDDPKMMSKIVTSLPAETVTNVQV
                     STLPLDQATVSVTKRVTDVVKDTRQHIAVVAGVPMSVPVVNAKPTRTPGVFHASFPGVP
                     SLTVSTVKGLPVSTNLPRGITEYKGRTAVPAGFTFGGGSHEAVIRFPKESGQKPIYVSV
                     TDVLTPAQVKQRQDEENRLQQEWNDTHPVEVAERNYEQARAELDQANKDVARNQERQAK
                     AVQVYNSRKSELDAANKTLADAKAEIKQFERFAREPMAAGHRMWQMAGLKAQRAQTDVN
                     NKKAAFDAAAKDKSDADAALGAALERRKQKENKEKDSKDKLDKESKRNKPGKATGKGKP
                     VGDKWLDDAGKDSGAPIPDRIADKLRDKEFKSFDDFRKKFWEEVSKDPELSKQFKDSNK
                     TNIQKGKAPFARKKDQVGGRERFELHHDKPISQDGGVYDMDNIRVTTPKRHIDIHRGK"
                     /name="colicin"
     gene            complement(421..954)
                     /locus_tag="IO770_23395"
     CDS             complement(421..954)
                     /locus_tag="IO770_23395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QOX49742.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTMLDRNYNATAAVYQKVVTLED
                     KLAEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(957..1883)
                     /locus_tag="IO770_23400"
     CDS             complement(957..1883)
                     /locus_tag="IO770_23400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QOX49743.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTAEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            2060..2458
                     /locus_tag="IO770_23405"
     CDS             2060..2458
                     /locus_tag="IO770_23405"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QOX49744.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKEIKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            complement(2652..3587)
                     /locus_tag="IO770_23410"
     CDS             complement(2652..3587)
                     /locus_tag="IO770_23410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QOX49745.1"
                     /translation="MSAALQYFEENLPLRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKICADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     AECRAIGRSIAKYTHRNFTPETFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDKRFMAL
                     CMLENGYSQKAIAAMLEVSTRTIRNWKSGK"
                     /name="replication initiation protein"
     gene            complement(4084..4227)
                     /locus_tag="IO770_23415"
     CDS             complement(4084..4227)
                     /locus_tag="IO770_23415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QOX49739.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     gene            complement(4289..4549)
                     /locus_tag="IO770_23420"
     CDS             complement(4289..4549)
                     /locus_tag="IO770_23420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="QOX49740.1"
                     /translation="MELKHSISDYTEAEFLEFVKKICRAEGATEEDDNKLVREFERLTE
                     HPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKPGFKQG"
                     /name="bacteriocin immunity protein"
     BGC             1..6079
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(2653..3587)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.261"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(961..1883)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cccccgccat tgccatggcc ggagccacct ccccagtgaa caccactacc agaaccgcca
       61 ccccatgggt tattttccga actccatccg gagccgtcag aagccccacc atttccacca
      121 agccccgtag ggccgccatt aatgttacca cctgtgttat gtgcgccact gttgtggtcg
      181 cgtccatctc caccgctcat aaaaattccc tcttttaagc gttaaaacaa ttaattacat
      241 agttaatact gtacataaaa ccactggttt tatgtacaga tcaaaaagta ctcgccatta
      301 ttttttctgt caagtcaggg ggggatattt tttggcagtt cggaggaatc actcatgaaa
      361 acggtgaacg gaacgcgaaa tcctttcgcg ggtcgtgagg cgttggacaa gcgaagcgcg
      421 tcagtttttc cggctcttgc ccgtgacagg tttctccgcc agtttatctt ccagtgtcac
      481 aactttctga tacaccgccg ctgtggcgtt gtagttcctg tcgagcattg ttttcattcc
      541 ccatccaagc acacagaaca taaccagaaa agccactgac accccaccca gccatttcca
      601 gctataccct ttaagggtgt tgtattcctg cacgatattg ctgtgaattg tcgcaatatc
      661 cctgttcagg gcatccgcgt ttgccttcat gtttgcgact gactcttcca gccgctgaaa
      721 atcctggctg atgcgctcac ctatcagccc tgcaacatcc agcttctgca cctcgtttct
      781 gacttcatcc ctgacctgtt ttgccacacc gtcagaaata tcccgatatc cgcgaagtgt
      841 ttcccttgcc acagccagag aatcagcaac agctttctgt tgctccattg cggtatgttg
      901 cagatgctcc accgataaca ggagcgtgag taatttttct tctgtgctgg ccatgttcag
      961 aatcccatag aaaatccacg tgacggtgat ttaacccgtt caggttcagt tcggttctgt
     1021 ctgctcactc tgtctctcat gtcaaagcca tgctcccgcg caacatcatg ttgcagcctt
     1081 ctgaccagtt catgttgctt aaccggagtg tccagccatt ccctgatgtc atgcccgacc
     1141 gttcgcgcct gttcaagatg gcgggaatag tccggttcag accgtttttg catgatgtca
     1201 tgacgctggg catcatcaag ccgtccggca gcttcaaaac ccagatgcac ttcgggacgt
     1261 cgttcaatgc cctgttcttt cagcgtccgg cagtcaatga agcccctttc aaagccatgc
     1321 tttctcatat gttcgttgtg cttcacctcc cagcgttttc gcaattcccg caactgctct
     1381 ttttgctgag tgagagtcag gcttccggaa tcctttttcg cacctccccg ctcgggatac
     1441 ctggcgttat agcgccggaa atactgttcc ggtgtacgtt caatgccatc attcacacgc
     1501 tggctcatca tgatatgcgc atgaggctgt tcgccgccgt caatgctcgc tttcggattg
     1561 tgaatggcaa aactccaggc gtgccgttcg ccagcttcct gacgtacaaa atcctgcacc
     1621 agttcaaggc gctgttccgc tgtcagttca cggggtaatg caatttcaag ttcccgatag
     1681 gtcgaaccgt tcgcacgttc aaactggtca gcagcctgcc agaaatgtga gggattatct
     1741 cgcgcccatt ccggcatgtt tccgtgtgcg gaatattcca aatcctgacg gtcgcggtat
     1801 ttctctttgc gctcgatgta atcggcgtgg tttgcagcct gccccttgcc accaaattta
     1861 acgctgaggt gataggttgc cacggtttca gccctccgca ggagcgcact gtttctcgac
     1921 gaaggagaga aacatataag tgcgcttcct tctaatttta cgaattagca cggaagatac
     1981 cccctgcacc cgccgaccgc aagcgatata ctgcacgacc acaacacacc gcaaaaaaac
     2041 accagggagc accacgaaca tgcgcatcag caacatcgaa tggctgaaga aacggatcgg
     2101 attcatcagg aagctcggag aacagaccgc acgacagaga cagattattg acctgctcga
     2161 caacgaagca ggactcacag aacaggaacg caaactcctc cacgtactgg caacagcgga
     2221 aaaaaacgac ttacaggcac aggagagcga acgaaaacag gcggtccaaa aacgaatcga
     2281 gggaaaaaaa caacgacgag aacgcaatca ccgactcttt ctcgcggctg ggctactgat
     2341 cgaagcagga ctggtagaca caaaaacagg cgagctatgc tataaaaaag acaggatatt
     2401 acaggcactt aaggaaatta agtacgatct ggagacatcc ccaaacccag acgcgtaagt
     2461 gcctccatcc gtgttccggt tccctggtgc ttcctcgctc attcgctcgc tacgctcggt
     2521 catgagactg cggcgagcac taccagcaca atcatgcgga aaacagcacc attgcagggc
     2581 tgaacgggga aaaacggcaa aagacctacg gctcaaaaaa acgccagaaa ggcgctgtta
     2641 tctgatatag gctattttcc gcttttccag tttcgaatgg ttcgagtaga aacctccaac
     2701 atcgccgcaa tagctttctg agaatatcca ttctccagca tacatagcgc catgaaacgc
     2761 ttgtcatcgt aggcttcgcc tttagcgatg ccacctttcc tgcctctctt ggcctgtatt
     2821 tctggcgtgt gcgtatcagc cacatactgt gcgaaagttt ccggcgtgaa gttcctgtgc
     2881 gtgtatttcg caatactcct gccaatagcc cggcattcag ctggtgaaag cggaacagga
     2941 agcgatgcgt tgtacatttc gacacgctga atcaccgcat caagccactg tgagaatgct
     3001 ggccatccct gacgaatcgc cctgtaagcc catttgcgcg tcatttcgaa caggtggcag
     3061 tttcgcccca tcccgtaatg tttatcgatg ctacgacgcg ctgaggcgct caaatcgaga
     3121 taatcagcca gttcatcgag ggtataggct tcctctcgcc actccatcac ctgccattcc
     3181 aggtggaacg gatttttgca aatcaggccg ctgtagttca catcagcaca aattttctca
     3241 cacagcgaac gttcaaccgc tgcggcatat ttcagcgcct taaccgaggc atcaggcgcg
     3301 gttctcacgg cgatgttgag tgcgtagagc aggtgagcgt gtccgttcac aggattttta
     3361 acggtgatgt tcggcgcggg ggcgttccgg tcgctccagt caatcgcagc cccctcgcgg
     3421 tcaacgtcaa aaaccagcca gaactgtgca tgaggctggt tctgctggat ataccgtgca
     3481 agaagcgcac gccctttacc ggagatacga aggccgaaag cgagatcatc cgtgtgatag
     3541 gggcggaggg gtaaattttc ttcgaagtat tgaagcgcgg cgctcatgtg ttacgattct
     3601 ctccgggtta tgcgacctac acgccctctg catttcatgt tgaatcttgc cggatctgga
     3661 catgatttca ggggcgtttg tttttcagtc gcttacacta cacccaacgg caaatcctgt
     3721 cgatccttcc cgaaaagccg atccatttac cataactccc gttgtacgcc ccccttaaaa
     3781 cgccctcagt cgctttctgg cgcaaaaaac gcaaaaagac aagaaaacac caggccaaac
     3841 cagaaaaacg cgtcagaatc gttttcaggg cgttgcataa ctatgcacga aagcgatgcg
     3901 cgatcgattt cgactgttcc gaagggcgaa caggtcaaaa tgacaagttt tctccccctg
     3961 ggtggaaagt tctgcacggg gattttagct ctcatctccc gcatggaatt tttgtgacat
     4021 ttcaaaaaaa agcgcatcag aatcagcctg acacgctttg tttttgtcaa agaggatttc
     4081 gggttactgc gttgctaatc cggtcacttc agctgttgat gacggggata cagtcccgcc
     4141 ctgaacatcc cggatatagt ttgcctgaca tgcagccaga ataatgactg caagaagcaa
     4201 taaaataatc cctgttattt ttttcataac gactccttgt tgtgaaagaa tgccagccat
     4261 tcatccataa acaaccggca ttcatatttt agccctgttt aaatcctggc ttaccattag
     4321 cagctcgcca ttctttaatt tctttaacaa tcccttcagg actatcttcc ctgtcatcgc
     4381 gaggataata aatcagatct gaaccatctg ggtgctcagt taatcgctca aactctctca
     4441 ctaatttatt gtcatcctct tcagtagcac cttcagctct acatattttt tttacaaact
     4501 ccagaaattc agcctcggta taatcactaa tactatgttt cagttccatt tttacttacc
     4561 tctatgaata tcaatatgtc gcttaggtgt ggtcactctg atattatcca tatcatagac
     4621 accaccatcc tgactgattg gtttatcatg atgtaattca aagcgttccc taccacctac
     4681 ttggtctttc ttccttgcaa aaggtgcttt tcccttttga atgttcgtct tattactgtc
     4741 tttaaattgc ttactaagct cgggatcttt tgacacttct tcccagaatt tcttccggaa
     4801 atcgtcaaaa cttttaaatt ctttatcacg caacttatca gcaatacgat caggaattgg
     4861 cgctcctgaa tctttacccg catcatcaag ccatttatca ccaactggtt ttcctttacc
     4921 cgtcgccttc cctggcttat tccgtttact ctccttatct aatttatcct tagagtcctt
     4981 ttctttattt tccttctgct tacggcgttc cagcgcagca cctaacgcag catcagcatc
     5041 agacttatct tttgcagcag catcaaatgc agccttctta ttatttacat ccgtctgtgc
     5101 ccgctgggcc ttaagccctg ccatttgcca cattcggtga ccagcagcca ttggttctcg
     5161 ggcaaatcgc tcgaattgtt ttatttcagc ctttgcatca gcaagagttt tattcgctgc
     5221 atcaagttca cttttacgag aattataaac ctgaacagct ttagcctgtc gttcctggtt
     5281 tctggcaaca tctttattag cctgatccag ttctgcgcgg gcctgttcat aattgcgttc
     5341 agccacttct acaggatgtg tgtcattcca ttcctgctgg aggcgatttt cctcatcctg
     5401 acgttgtttt acctgtgcag gggtaagaac atctgtcact gatacataaa tcggcttctg
     5461 cccactttct ttcgggaaac ggatcaccgc ttcatgtgaa ccaccaccaa aggtaaatcc
     5521 tgcaggaacg gcagtccggc ccttatactc cgtaatacca cggggaaggt ttgttgacac
     5581 aggaagacct ttgaccgtgc tgactgtcag agaagggaca ccaggaaatg atgcgtgaaa
     5641 tacacctggg gtacgtgttg gtttagcatt cacaacaggc acactcatag gtacacctgc
     5701 aactacggca atatgctgtc gtgtgtcctt cacaacatcg gtcacacgtt ttgtcacgct
     5761 aacagttgcc tggtccagcg gaagtgtact gacctgaaca tttgttactg tttctgccgg
     5821 taatgacgtc acaatctttg acatcatttt cgggtcatct tttgctattt cagatggcag
     5881 aatgccgtaa agcgcaatac cccatgcact gaatttaaac ggacctttca gggctgcaaa
     5941 gatatccgct attgccgcag ataaagcctc accagagacg gcgataccca gtgttccggc
     6001 accaggagct gccaaagcag gaaaaccaaa tgccactgct gctacggatg aattgctgcc
     6061 accaccggaa ttaccattc
//
