LOCUS       CP063735                1552 bp    DNA     circular BCT 15-OCT-2021
DEFINITION  Escherichia coli strain 18SC05VL02-EC plasmid pVPS18EC0505-7,
            complete sequence.
ACCESSION   CP063735
VERSION     CP063735.1
DBLINK      BioProject: PRJNA292663
            BioSample: SAMN16533951
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Tate,H., Li,C., Nyirabahizi,E., Tyson,G.H., Zhao,S.,
            Rice-Trujillo,C., Jones,S.B., Ayers,S., M'ikanatha,N.M., Hanna,S.,
            Ruesch,L., Cavanaugh,M.E., Laksanalamai,P., Mingle,L.,
            Matzinger,S.R. and McDermott,P.F.
  TITLE     A National Antimicrobial Resistance Monitoring System Survey of
            Antimicrobial-Resistant Foodborne Bacteria Isolated from Retail Veal
            in the United States
  JOURNAL   J Food Prot 84 (10), 1749-1759 (2021)
   PUBMED   34015113
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2020) CVM/OR, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JUN-2020
            Assembly Method                   :: HGAP v. 4
            Assembly Name                     :: Microbial Assembly
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 685x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/27/2020 13:00:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.13
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,435
            CDSs (total)                      :: 5,309
            Genes (coding)                    :: 5,075
            CDSs (with protein)               :: 5,075
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 81 of 234
            Pseudo Genes (incomplete)         :: 146 of 234
            Pseudo Genes (internal stop)      :: 46 of 234
            Pseudo Genes (multiple problems)  :: 34 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="18SC05VL02-EC"
                     /isolation_source="Veal"
                     /db_xref="taxon:562"
                     /plasmid="pVPS18EC0505-7"
                     /geo_loc_name="USA:SC"
                     /collection_date="2018"
                     /collected_by="CVM"
     gene            complement(898..1374)
                     /locus_tag="IO772_01215"
     CDS             complement(898..1374)
                     /locus_tag="IO772_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QOX35546.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVRE
                     LLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKNAL
                     VRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(213..305)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.426"
                     /coverage="100.0"
                     /evalue="4.39e-26"
     Typing          1454..1552
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.0"
                     /coverage="91.0"
                     /evalue="3.29e-43"
     Typing          408..668
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.511"
                     /coverage="100.0"
                     /evalue="3.95e-106"
ORIGIN
        1 gtaaaagatc tattctaggc aagggtgttg cgcgcaacgc aacgcccttt gggggtgtcg
       61 gggcgaagcc ctgacccagt cacgtagcga tagcggagtg tatactggct tactcctctg
      121 caagcctcgc cagccgtgat ttttgccaga tttcgtggtt ttcacgttca acagctcctg
      181 cacgtttttt gccgtccgtt caccgttaac ccccgcagaa atcgggacag gatgtgcaaa
      241 tgtaataccg tcacccgcga cgatattccg tttgccatct ggtcagggct tcgccccgac
      301 accccgtatc agtgcgtacg tgctcaatgt ggtttccggt tctcttccgc ttcctcgccc
      361 actcgctcac taacgttcgg tcgctggggc tgcggcgagc ggtgccacct ctcgcatgtt
      421 gtcggtgatt ccgctgttgt tctttcgggg atgagtcgtc agatagccct tcaaacgcgc
      481 tcagaagctc tcagacgcac gaggcggggt ttaaatgccc gtcagggcat ggaaggcgcg
      541 aaaaacgctt acagagcgtt tcaggaggtc aggcgcgccg atacccctag ccaggggctt
      601 tcaggtcgcc aggaaccgcg ccagccccct gcaaagccca tttgcacggt acaggcagtc
      661 actcggttgg taggtggtcg tagaccgtcg gtgttacagc gatgggggtc tggggttctc
      721 cccagacggt ttccggttgc gctcaaggcg ctgccggaac cgtggcggct cggtcgttct
      781 ggagatcggg gagcaaaaac acgcaaggga ttttgaggcg gcaaaagagc aatgcaggat
      841 agacaatcat ggtcagaacg ttaacacgta gcccctgatt gtccgcctgc cgctggctca
      901 atttgaaaac atggccatga ttcggcgctg aatatcaccg tctttgttga ccagtgtttt
      961 agtgacaaat gctgcatggt ttttcgtagt gttgtatccc ttccagactt catgtggatc
     1021 gagggcatac gcacaaacct taccagcaac ggggattcta actaatgcat tttttgattc
     1081 taagtaattg agccaacgaa taattgtgct tctgctaact ccaagttttt cagctaggaa
     1141 ttgctgatcc gcaacgacag cgccacacgt aggatcgata tgctccgcta aaaatgagta
     1201 cagcttcagt gctgatatgc cttggctatc cttcagcagc tctcgaacgc gttcccaacc
     1261 ttttggggat acctgtgtaa accttgaatt cttctttgct tcttcgagat cctcggtgtt
     1321 tttctttgcc tcatctgaaa gttgtttcag tcgtttttct gaatcaataa acatgatttt
     1381 ttccctctaa aaatcccatg cactaacgtt ggttcaaatc taagccatca tgacacatct
     1441 gtgcaacacc ttgttgcgcg ttgtcgcatc ctggatgcga caacgttgtg tcaccctgga
     1501 tgcgacaggt aaacaaaaaa cacactaaaa aaacaacaac ttacaaatcg gc
//
