LOCUS       CP063733                6647 bp    DNA     circular BCT 15-OCT-2021
DEFINITION  Escherichia coli strain 18SC05VL02-EC plasmid pVPS18EC0505-4,
            complete sequence.
ACCESSION   CP063733
VERSION     CP063733.1
DBLINK      BioProject: PRJNA292663
            BioSample: SAMN16533951
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Tate,H., Li,C., Nyirabahizi,E., Tyson,G.H., Zhao,S.,
            Rice-Trujillo,C., Jones,S.B., Ayers,S., M'ikanatha,N.M., Hanna,S.,
            Ruesch,L., Cavanaugh,M.E., Laksanalamai,P., Mingle,L.,
            Matzinger,S.R. and McDermott,P.F.
  TITLE     A National Antimicrobial Resistance Monitoring System Survey of
            Antimicrobial-Resistant Foodborne Bacteria Isolated from Retail Veal
            in the United States
  JOURNAL   J Food Prot 84 (10), 1749-1759 (2021)
   PUBMED   34015113
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2020) CVM/OR, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JUN-2020
            Assembly Method                   :: HGAP v. 4
            Assembly Name                     :: Microbial Assembly
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 685x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/27/2020 13:00:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.13
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,435
            CDSs (total)                      :: 5,309
            Genes (coding)                    :: 5,075
            CDSs (with protein)               :: 5,075
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 234
            CDSs (without protein)            :: 234
            Pseudo Genes (ambiguous residues) :: 0 of 234
            Pseudo Genes (frameshifted)       :: 81 of 234
            Pseudo Genes (incomplete)         :: 146 of 234
            Pseudo Genes (internal stop)      :: 46 of 234
            Pseudo Genes (multiple problems)  :: 34 of 234
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="18SC05VL02-EC"
                     /isolation_source="Veal"
                     /db_xref="taxon:562"
                     /plasmid="pVPS18EC0505-4"
                     /geo_loc_name="USA:SC"
                     /collection_date="2018"
                     /collected_by="CVM"
     gene            complement(join(5746..6647,1..667))
                     /gene="cea"
                     /locus_tag="IO772_01130"
     CDS             complement(join(5746..6647,1..667))
                     /gene="cea"
                     /locus_tag="IO772_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="QOX35535.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGGSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            927..1364
                     /locus_tag="IO772_01135"
     CDS             927..1364
                     /locus_tag="IO772_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QOX35536.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="entry exclusion protein 1"
     gene            1445..1864
                     /gene="exc2"
                     /locus_tag="IO772_01140"
     CDS             1445..1864
                     /gene="exc2"
                     /locus_tag="IO772_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QOX35537.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            complement(2182..2415)
                     /gene="mbeD"
                     /locus_tag="IO772_01145"
     CDS             complement(2182..2415)
                     /gene="mbeD"
                     /locus_tag="IO772_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QOX35538.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="mbeD"
     gene            complement(2422..2940)
                     /locus_tag="IO772_01150"
     CDS             complement(2422..2940)
                     /locus_tag="IO772_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbeB"
                     /protein_id="QOX35539.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="mobilization protein MbeB"
     gene            complement(2918..3676)
                     /locus_tag="IO772_01155"
                     /pseudo=""
     CDS             complement(2918..3676)
                     /locus_tag="IO772_01155"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3618..3941)
                     /locus_tag="IO772_01160"
     CDS             complement(3618..3941)
                     /locus_tag="IO772_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QOX35531.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4005..4196
                     /locus_tag="IO772_01165"
     CDS             4005..4196
                     /locus_tag="IO772_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QOX35532.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5223..5360)
                     /locus_tag="IO772_01170"
     CDS             complement(5223..5360)
                     /locus_tag="IO772_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QOX35533.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            5408..5749
                     /locus_tag="IO772_01175"
     CDS             5408..5749
                     /locus_tag="IO772_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="QOX35534.1"
                     /translation="MSLRYYIKNILFGLCCTLIYIYLITKNSEGYYFLVSDKMLYAIVI
                     STILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 immunity protein"
     Typing          complement(4282..4369)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          4380..4938
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.544"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2079..3628)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tactggagct taaacgagaa ttgagagtct taatctctcc atccattttc accacttcag
       61 attgtgcagc agaaagtttt ttttgggcga tctcaacagc tttagcttct tcactcaatg
      121 cagccagtcg tttctcttca gcttcagcca gtttcaactg gcgttctgtt tcagccttct
      181 cccgttcaat ctctttacgt cgttgttctg cttcctgaaa agccttttct gctgcttccg
      241 cttctttacg ggctttttct tctgctttcg caaggcgcaa acgctctgct tccgcctgca
      301 tagctgcatt attagcatga gcaagctctg ttgctgaagg cgtacgtgag gcattgtgac
      361 gaagagcctc attcacgata tccttcaggc gctgagtcag cgcatccctg tttgcctttg
      421 ctttcgcctg tgcttccgct gcagcttttg cccgggcagc ctgctctgcc tgtgttttct
      481 ttaattgagc agtagaccat ttagcagttg catgaatagc tgcagaactt tcacttttac
      541 tgcctccttt tccacctccg ccgccagagc cactcccgtc aggagtacca ttcaaaagag
      601 taataattac ctgtccctta tcatcataag gaacaccatc tttatagtac gctaccgcgg
      661 tttccattat aaaatcctct ttgactttta aaacaataag ttaaaaataa atactgtaca
      721 tataaccact ggttttatat acagcataaa agctacgccg ctgcattttc cctgtcaaga
      781 ctgtggactt ccatttttgt gaaaacgatc aaaaaaacag tctttcacac cacgcgctat
      841 tctcgcccga tgccacaaaa accagcacaa acattaccgt tctcagacct cattatgttt
      901 tactgaaact atgagatgag acatctatgg gacactgtca ctttatggca tggcacacac
      961 tccgggacgc actaaaaatg acaggcagat cgcgttcaca gttttaccgt gatatgcgcg
     1021 caggccttgt cagttaccgt accggcaggg acggacgacg ggagtttgaa accagtgaac
     1081 tgatccgggc atacggcgaa ttaaagcaga atgagacacc agaaaggcac agtgagggac
     1141 atgcagaaaa tccacatgat cagcagacag aacgcattct ccgggaactg aatgagctga
     1201 aacaatgcct gacgctgatg cttgaggata aacaggcaca ggatatggat cgcagacgcc
     1261 aggaagcaga acgggaacag ctacaaaatg agatagccca gctcaggcag gcactggaac
     1321 tggaaaagaa acggggattc tggtccaggt tgttcggtcg ctgaacgctg tcagagactg
     1381 atgataaaat agtcttcgga taataactca ccgagaataa atactttaag gtagggagac
     1441 actcatgaga cgtaccggaa acaaactttg tcttatcgcc atgataacag caacagtagc
     1501 tctcacagcc tgtaccccaa agggcagcgt ggaacaacat acccggcatt acgtatatgc
     1561 ttctgatgac ggttttgatc ccaacttttc cacccaaaaa gccgacacaa cacgaatgat
     1621 ggtgcctttt tttcggcagt tctgggatat gggagctaaa gataaagcga caggaaaatc
     1681 acggagtgat gtgcaacaac gcattcagca gtttcacagc caggaatttt taaactcact
     1741 ccggggcaca actcaatttg cgggtactga ttaccgcagc aaagacctta ccccgaaaaa
     1801 atccaggctg ctggctgaca cgatttctgc ggtttatctc gatggctacg agggcagaca
     1861 gtaagtggat ttaccataat cccttaattg tacgcaccgc taaaacgcgt tcagcgcgat
     1921 cacggcagca gacaggtaaa aatggcaaca aaccacccga aaaactgccg cgatcgcgcc
     1981 tgataaattt taaccgtatg aatacctatg caaccagagg gtacaggcca cattaccccc
     2041 acttaatcca ctgaagctgc catttttcat ggtttcacca tcccagcgaa gggccatcca
     2101 gcgtgcgttc ctgtatttcc ggttgacgct cccgttccag ggataacaca tgttcgcgct
     2161 cctgtatcag ccgttcctct cttatctcca gttctcgctg tataactggc tcaagcgttc
     2221 tgtctgctcg ctcaagtgct gcacctgctg actcaactgc atgacccgct cgttcagcat
     2281 cgcgttgtcc cgttgcgtaa gcgaaaacat cttctgcaat tccacgaagg cgctctccca
     2341 ttcgctcagc cgctgcatat agtcctgttg cagctgctct aaggcgttca gcaaatgtgt
     2401 ttccagctct gtcactctgt gtcactcctt cagatgtacc cactctttcc cctgaaaggg
     2461 aatcacctcc gctgatttcc cgtacggaag gacaaggaat ttcctgttcc cgtcctgcac
     2521 aaactccacg ccccatgtct tcgcgttcag tttctgcaat gtctcttcct gcttcctgat
     2581 ttcttccagg ttcgcctgta tcctccctcc aagataccag agcgtcccgc cactcgcggt
     2641 aaacaggaga aagactatcc ccagtaacat catgcccgta ttccctgcca gctttaacac
     2701 gtccctcctg tgctgcatca tcgcctcttt caccccttcc cggtgttttt ccagcgattc
     2761 ctctgtcgag gctgtgaaca gggctatagc gtctctgatt ttcgtctcgt ttgatgtcac
     2821 agcctcgctt acagattcgc cgagcctcct gaactcgttg ttcagcattt tctctgtaga
     2881 ttcggctctc tctttcagct ttttctcgaa ctccgcgccc gtctgcaaaa gattgctcat
     2941 aaaatgctcc tttcagcctg atattcttcc cgccgttcgg atctgcaatg ctgatactgc
     3001 ttcgcgtcac cctgaccact tccagccccg cctcagtgag cgcctgaatc acatcctgac
     3061 ggccttttat ctctccggca tggtaaagtg catctatacc tcgcgtgacg ccctcagcaa
     3121 gcgcctgttt cgtttcaggc aggttatcag ggagtgtcag cgtcctgcgg ttctccgggg
     3181 cgttcgggtc atgcagcccg taatggtgat ttaccagcgt ctgccaggca tcaattctag
     3241 gcctgtctgc gcggtcgtag tacggctgga ggcgttttcc ggtctgtagc tccatgttcg
     3301 gaatgacaaa attcagctca agccgtccct tgtcctggtg ctccacccac aggatgctgt
     3361 actgattttt ttcgagaccg ggcatcagta cacgctcaaa gctcgccatc actttttcac
     3421 gtcctcccgg cggcagctcc ttctccgcga acgacagaac accggacgtg tatttcttcg
     3481 caaatggcgt ggcatcgatg agttcccgga cttcttccgg attaccctga agcaccgttg
     3541 cgccttcgcg gttacgctcc ctccccagca ggtaatcaac cggaccactg ccaccacctt
     3601 ttcccctggc atgaaattta actatcatcc cgcgccccct gttccctgac agccagacgc
     3661 agccggcgca gctcatcccc gatggccatc agtgcggcca ccacctgaac ccggtcaccg
     3721 gaagaccact gcccgctgtt caccttacgg gctgtctgat tcaggttatt tccgatggcg
     3781 gccagctgac gcagtaacgg cggtgccagt gtcggcagtt ttccggaacg ggcaaccggc
     3841 tcccccaggc agacccgccg catccatacc gccagttgtt taccctcaca gcgttcaagt
     3901 aaccgggcat gttcatcatc agtaacccgt attgtgagca tcctctcgcg tttcatcggt
     3961 atcattaccc catgaacaga aatccccctt acacggaggc atcagtgact aaacaggaaa
     4021 aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctg ctggagaagc
     4081 tcaacgaact ggacgcagat gaacaggccg atatttgtga atcgcttcac gaccacgccg
     4141 atgagcttta ccgcagctgc ctcgcacgtt tcggggatga cggtgaaaac ctctgacaca
     4201 tgcagctccc ggagacggtc acagcttgtc tgtgagcgga tgccgggagc tgacaagccc
     4261 gtcagggcgc gtcagcaggt tttagcgggt gtcggggcgc agccctgacc cagtcacgta
     4321 gcgatagcgg agtgtatact ggcttaacca tgcggcatca gtgcggattg tatgaaaagt
     4381 acgccatgcc gggtgtgaaa tgccgcacag atgcgtaagg agaaaatgca cgtccaggcg
     4441 cttttccgct tcctcgctca ctgactcgct acgctcggtc gttcgactgc ggcgagcggt
     4501 actgactcac acaaaaacgg taacacagtt atccacagaa tcaggggata aggccggaaa
     4561 gaacatgtga gcaaaagacc aggaacagga agaaggccac gtagcaggcg tttttccata
     4621 ggctccgccc ccctgacgag catcacaaaa atagacgctc aagtcagagg tggcgaaacc
     4681 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     4741 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     4801 tttctcatag ctcacgctgt tggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     4861 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     4921 ttgagtccaa cccggtaagg cacgccttaa cgccactggc agcagccact ggtaaccgga
     4981 ttagcagagc gatgatggca caaacggtgc tacagagttc ttgaagtagt ggcccgacta
     5041 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     5101 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gttggtagcg gtggtttttt
     5161 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttaatctt
     5221 ttctactgaa ccgcgatccc cgtcagttta gaagaggagg atggtgcgat ggtccctccc
     5281 tgaacatcac gtatatagtt agcctgacat ccaacaagga ggtttatcgc gaatattccc
     5341 acaaaaaatc ttttcctcat aactcgatcc ttataaaatg aaaagaatat atggcgaggt
     5401 ttaatttatg agcttaagat actacataaa aaatatttta tttggcctgt gctgcacact
     5461 tatatatata taccttataa caaaaaacag cgaagggtat tatttccttg tgtcagataa
     5521 gatgctatat gcaatagtga taagcactat tctatgtcca tattcaaaat atgctattga
     5581 atacatagct tttaacttca taaagaaaga ttttttcgaa agaagaaaaa acctaaataa
     5641 cgcccccgta gcaaaattaa acctatttat gctatataat ctactttgtt tggtcctagc
     5701 aatcccattt ggattgctag gactttttat atcaataaag aataattaaa tccctaacac
     5761 ctcatttata gtattaagtt tattcttatc aatataggag catagaattc ctgtaacaat
     5821 agcaataccc caaataccta atgtagttcc agcaagcaag ctaaaaagta aagcaacaac
     5881 ataactcccc cctgcatctg cagctttctt ctctaatgta agaaatagtg gcttccagtc
     5941 acctgtatcc ttaattttta ggatatcaga taccacatca tatccaaaag aaacatgccc
     6001 cgtaatcttc aagtacttgg caaactgatc taaatgttta gcccagtcat catacttcac
     6061 cgatgccaac gcattaaaaa tagcatcacg atcggctttg ctgaatttct tatttaaaac
     6121 atccttgtat ttttcaaaag cagcgagagc ttcattcaca ttgccgattt tcttaccttt
     6181 agacttatca gcaagttcct gtgccatttt cgaatatttt tcaccatatt tttcagtcag
     6241 cgtttgataa aagctaactg ttgcatcaac agcatcctta atctgtgaat taaggagatt
     6301 attctgtgct tttttcaaat tttcttcagc ttcatgaaca cgagcgatac cggcattacg
     6361 attattactg acctgagaaa tagccttctg gatctgagtt atatcagcat ttatccggtt
     6421 aatacgtgtt tctgatgctg ttacctgttt ttgtttttct tctctaatct taccggcccc
     6481 aacccgtcgt ctggttgctt caaaaaaagg acggttctga agcggatcat tggctcttgg
     6541 tgatagtttt ttgaccagct catccagttc tttatattta gcggatgcct gagccagttc
     6601 atttcgtttt ccagcgagcg ttttcatttc tgcatcacgg gcatgga
//
