LOCUS       CP063727                4091 bp    DNA     circular BCT 15-OCT-2021
DEFINITION  Escherichia coli strain 18MD05VL07 005213EC plasmid pVPS18EC0676-4,
            complete sequence.
ACCESSION   CP063727
VERSION     CP063727.1
DBLINK      BioProject: PRJNA292663
            BioSample: SAMN16533952
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Tate,H., Li,C., Nyirabahizi,E., Tyson,G.H., Zhao,S.,
            Rice-Trujillo,C., Jones,S.B., Ayers,S., M'ikanatha,N.M., Hanna,S.,
            Ruesch,L., Cavanaugh,M.E., Laksanalamai,P., Mingle,L.,
            Matzinger,S.R. and McDermott,P.F.
  TITLE     A National Antimicrobial Resistance Monitoring System Survey of
            Antimicrobial-Resistant Foodborne Bacteria Isolated from Retail Veal
            in the United States
  JOURNAL   J Food Prot 84 (10), 1749-1759 (2021)
   PUBMED   34015113
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2020) CVM/OR, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JUN-2020
            Assembly Method                   :: HGAP v. 4
            Assembly Name                     :: Microbial Assembly
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 457x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/27/2020 13:20:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.13
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,057
            CDSs (total)                      :: 4,934
            Genes (coding)                    :: 4,826
            CDSs (with protein)               :: 4,826
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 43 of 108
            Pseudo Genes (incomplete)         :: 68 of 108
            Pseudo Genes (internal stop)      :: 23 of 108
            Pseudo Genes (multiple problems)  :: 23 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="18MD05VL07 005213EC"
                     /isolation_source="Veal"
                     /db_xref="taxon:562"
                     /plasmid="pVPS18EC0676-4"
                     /geo_loc_name="USA:MD"
                     /collection_date="2018"
                     /collected_by="CVM"
     gene            complement(join(2636..4091,1..692))
                     /locus_tag="IO773_01455"
     CDS             complement(join(2636..4091,1..692))
                     /locus_tag="IO773_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QOX30753.1"
                     /translation="MAIYHLSMKILSRSKGYSAVASAAYRAGEKILDERTGVIHDYTRK
                     NGVASAVILTPANAPAWCANRAELWNAVEKAERRKNSQLAREFELAIPRELAQDAARET
                     VLNFVRENFVSRGMIADVAFHNLGGSNPHAHIMLSMRAITPDGFGEKVREWNDWTHAET
                     WRGSWADHANRALANAGYQEEIDHRSYERQGVEKTPGIHLGKSACAMEKWGIETERGEQ
                     NRLINRLNLEIQVSRDELRNSELTEPARRMADLLNIELPDNATSYILHDIIEALPTDSN
                     AAWKLTSKAMSMMADMQATRRRWEDLNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWV
                     APEYGREQEKIQSLSQRMEKLRQHHWKVEKQDIPASQKAFETQWQQWGASGLADLREQL
                     RKREEELRRKEQEETERRRAEQLRRNDNAVMDSGVLKAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRARIFESVDLMRDRSGYICERSEIGQRPPLQHVYSSATFEQ
                     LLTQVCQTWPQHTRDLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSMGGVVRLNGTLYPAFAVPTADNSQLVINALTDKGLRFA
                     GYGEAVNHDADSPARPAPELMQFHLKTWSEPLFAIVNTPEKQPDHLFRSLGFERTWDEW
                     QRDEYARTHATERRHDRGWSQ"
                     /name="MobA/MobL family protein"
     gene            863..1153
                     /locus_tag="IO773_01460"
     CDS             863..1153
                     /locus_tag="IO773_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="QOX30754.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     GETQLRAWLLHLLKTEVTRQADVSRIQPLIDELNALPKPVPKEVSENDQQA"
                     /name="mobilization protein"
     gene            1686..2387
                     /locus_tag="IO773_01465"
     CDS             1686..2387
                     /locus_tag="IO773_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QOX30755.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGSDAESTEL
                     QKTMTVTAEEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCVDELNQKSDLAVTLETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     Typing          338..692
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          709..859
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.81"
                     /coverage="85.0"
                     /evalue="3.53e-55"
     Typing          1687..2386
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.301"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(4..734)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.59"
                     /coverage="99.0"
                     /evalue="1.84e-160"
     Typing          complement(2640..4090)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.793"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 aggttaagcc tgttaatcag gcgattctgt tcgccgcgtt ctgtttcgat gccccacttt
       61 tccatcgcac aggccgactt tccgaggtga atgccaggcg ttttctcaac cccctgtcgc
      121 tcgtatgaac gatggtctat ctcttcctga taaccggcgt tcgccagcgc cctgttcgca
      181 tggtctgccc atgatccccg ccatgtttcg gcgtgagtcc agtcattcca ctcacggact
      241 ttctccccga agccgtcagg cgtgattgcc ctcatggata gcatgatgtg cgcgtgtggg
      301 tttgacccgc ccagattgtg aaaggccacg tcggcgatca tgccgcgact gacaaagttt
      361 tcccttacga agttgagaac tgtttcccgt gcggcatcct gcgccagttc acgggggatg
      421 gcgagttcga actcccgcgc aagttgtgag tttttccgcc gctcggcttt ttcaacagca
      481 ttccagagtt cggcgcggtt cgcacaccat gctggggcgt ttgctggcgt cagaatcact
      541 gcgctggcta caccgttttt gcgggtgtag tcgtgaatca ctccggtgcg ctcatccagt
      601 attttttctc cggcacgata ggcagcagaa gcaacggcgc tgtagccctt gcttctggaa
      661 agaatcttca tgctgagatg ataaattgcc atcgtgtcgt tgttgccgtt ttcttttagc
      721 cccgcagggc gcacacaaac tcgacgaagg agagtttgta taagtgcgca cttcgtgatt
      781 tgctgtgcat tatgccagtt cagtttttaa actaccagtg acgcgctaaa atgcgatttt
      841 tcaccgcgca cggagttacg gaatgagtag aacactcgaa cagaagattg ccgatgctga
      901 ggccagattg cagcgtctga aggcgaagag caggagcctg gacacagcgc agaaggtcat
      961 tgtgggagcg gcattgctgg caaaggtcag gaagcctggg gaaacacaac tacgagcctg
     1021 gttgcttcat ctcctgaaga cagaggtaac acggcaggct gatgtgagca ggatacagcc
     1081 gttgattgac gaactgaacg cgttaccaaa accagtcccc aaagaagtgt cagaaaacga
     1141 ccaacaggcg tgaatgcacc gtccgctcgc cgcagcccca gcgaccgagc gttagcgagc
     1201 gagtgggcga ggaagcggaa gtgaatccgt cgccagattg agcacatacg cacggttttt
     1261 cgacaggtga tatgcgctga acccgcatga atactggatt tccacagggt tgagcggggt
     1321 tttgttaaag cttgcgaacc tgcttttttg taacctcttg tttttcatga ttttttgttt
     1381 tgaacacccc aagttttcca caggctcgcc gttaagtttt tagatcttgt ttttaagatc
     1441 ttgtttttaa tacttgtttt agggtcgaat tttcttatga aattcaagag tttagaagac
     1501 gataggtaat ataatttaag gtaaaggcaa tatgatttaa ggtggatagg caatatgatt
     1561 taaggtaaag gcaatatgat ttaaggtaat attcacagga aaagggatat taaaattaaa
     1621 tcatattgcc tttacctaaa ggaatgttta tattttactc attaccttag gttgagggat
     1681 ttacaatgtc ggatctgatt gcctacaaat ccaatgcact ggttgaggcc agctataaat
     1741 tgactcttca ggaacagcga ttcttgttac tttgcattgg tcgattgaag tcgggttctg
     1801 atgcagaatc cactgaattg caaaagacca tgactgttac cgccgaagag tatttcgact
     1861 cattccctga tatggggcgg aaaaatgccg aggttcagct acaggaagca attgatcgtt
     1921 tgtgggacag gtcgatcatc cttaaggacg atgagaagcg cgaggagttc cgctggatcc
     1981 agtatcgggc acagtacgct aaaggtgagg gcaaagccca aatcactttt tcagatgcgg
     2041 tcatgcctta tctgacacag ttgaaggggc aatttactag agttgtaatt aagaacatct
     2101 ccaatttatc aagaagttac agcataagaa tatacgaact tctccagcaa ttccgcagca
     2161 caggggaacg tattatcgcc ctcgatgatt ttcgctcaat gcttgatata gagcataaat
     2221 atcaaacata taagtctctg aaccagcaat tattaagacc ttgcgttgac gaactgaatc
     2281 aaaaaagcga cttggctgta acgcttgaga cgatcaagaa ggggcgcacg gttgtagccc
     2341 tgcattttcg cttcaaggaa gacaaacaga tcaagatgac gatctagaat cgattttaag
     2401 gcctctaaaa cgctctctga cgcgtttttt ttgtgtttgg catgcgaagg tactggtgaa
     2461 gtaaaaaatt gcgtcataaa cgctctggca cagtgaaaaa agaaccccca atttttcggg
     2521 ggcgggaaaa ctataaagtt gatttgtgtt atgtgtgtgc ttcttgcatt catccctgtc
     2581 gatgaacatc gacgaacata acacgatgtt aaacaaaaca aaatattggt cacattcact
     2641 gtgaccaacc gcgatcatgt ctcctctcgg tggcgtgtgt tcgggcatat tcatcacgtt
     2701 gccattcatc ccaggtgcgc tcaaagccca gcgagcggaa cagatgatcc ggctgttttt
     2761 ctggcgtgtt gacgatagca aaaagcggtt cgctccaggt tttcagatga aactgcataa
     2821 gttccggcgc aggacgtgcc ggactgtcag catcgtggtt taccgcttca ccatagcctg
     2881 caaagcgtaa acctttgtcg gtcagtgcat tgattaccag ctggctgttg tcggctgtgg
     2941 ggacggcaaa cgcgggatac agcgtaccat taagccggac aacaccgccc atagagtcat
     3001 ccacacccat gaccagcaaa agctgaagct ggcgggtttc acggtcgaca gaactcatta
     3061 ccggcatcag agtcggtggc agacgtccct gcgtaaaatc caccttcctt gccgccagtg
     3121 atggcatggc tggctggcgg tcttctccaa gacagaaaga ctccggccat gtcttcggct
     3181 ggcggagatc gcgggtatgt tgcggccatg tctggcaaac ctgcgtcagc agttgctcaa
     3241 aggtcgcaga ggaataaaca tgttgcagtg gtggacgttg accgatttcg cttcgctcac
     3301 agatataacc actcctgtca cgcattaaat caacactctc aaaaattctg gcgcggtatt
     3361 tctccagttc gttcccccag agcgtatatt ctccgttccg gttgtgaagc agaaggtaac
     3421 aggtcatttc cccgttacct ggcatggggg cctcgccata ccccgtaacg actgctttca
     3481 gtacccctga atccataacg gcattgtcat ttctcctgag ctgctccgcc cgacgacgtt
     3541 ctgtttcctc ctgctccttc cggcgcaatt cctcttcacg tttacgcaac tgttctcgga
     3601 gatctgcaag gccagatgca ccccactgct gccactgtgt ttcaaatgct ttttgactcg
     3661 cgggaatgtc ctgtttctca accttccagt gatgctgccg caatttttcc atgcgctggc
     3721 tcaacgactg gattttttcc tgttcgcggc cgtattccgg tgcaacccat tgcatcaggg
     3781 gaatacggga aaaaccggac gaaaacggcg atgatttttt caggctggca gcatggctca
     3841 tcgcttcctt tcgttcggtg ttcagatctt cccagcgtct gcgcgtcgcc tgcatatctg
     3901 ccatcatcga catggcttta ctggtgagtt tccatgctgc gttactgtcc gtcggcaacg
     3961 cttcgatgat gtcatgcagt atgtacgaag ttgcgttgtc aggaagctca atattcagaa
     4021 ggtcagccat acggcgtgcg ggctctgtca gctcactgtt cctgagttcg tcgcgggaaa
     4081 cctgtatttc c
//
