LOCUS       CP063725                1552 bp    DNA     circular BCT 15-OCT-2021
DEFINITION  Escherichia coli strain 18MD05VL07 005213EC plasmid pVPS18EC0676-7,
            complete sequence.
ACCESSION   CP063725
VERSION     CP063725.1
DBLINK      BioProject: PRJNA292663
            BioSample: SAMN16533952
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Tate,H., Li,C., Nyirabahizi,E., Tyson,G.H., Zhao,S.,
            Rice-Trujillo,C., Jones,S.B., Ayers,S., M'ikanatha,N.M., Hanna,S.,
            Ruesch,L., Cavanaugh,M.E., Laksanalamai,P., Mingle,L.,
            Matzinger,S.R. and McDermott,P.F.
  TITLE     A National Antimicrobial Resistance Monitoring System Survey of
            Antimicrobial-Resistant Foodborne Bacteria Isolated from Retail Veal
            in the United States
  JOURNAL   J Food Prot 84 (10), 1749-1759 (2021)
   PUBMED   34015113
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2020) CVM/OR, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JUN-2020
            Assembly Method                   :: HGAP v. 4
            Assembly Name                     :: Microbial Assembly
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 457x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/27/2020 13:20:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.13
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,057
            CDSs (total)                      :: 4,934
            Genes (coding)                    :: 4,826
            CDSs (with protein)               :: 4,826
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 43 of 108
            Pseudo Genes (incomplete)         :: 68 of 108
            Pseudo Genes (internal stop)      :: 23 of 108
            Pseudo Genes (multiple problems)  :: 23 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="18MD05VL07 005213EC"
                     /isolation_source="Veal"
                     /db_xref="taxon:562"
                     /plasmid="pVPS18EC0676-7"
                     /geo_loc_name="USA:MD"
                     /collection_date="2018"
                     /collected_by="CVM"
     gene            complement(868..1344)
                     /locus_tag="IO773_00630"
     CDS             complement(868..1344)
                     /locus_tag="IO773_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QOX30610.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVRE
                     LLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKNAL
                     VRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(183..275)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.426"
                     /coverage="100.0"
                     /evalue="4.39e-26"
     Typing          1424..1532
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.95e-50"
     Typing          378..638
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.366"
                     /coverage="100.0"
                     /evalue="3.98e-101"
ORIGIN
        1 cgcgcaacgc aacgcccttt gggggtgtcg gggcgaagcc ctgacccagt cacgtagcga
       61 tagcggagtg tatactggct tactcctctg caagcctcgc cagccgtgat ttttgccaga
      121 tttcgtggtt ttcacgttca acagctcctg cacgtttttt gccgtccgtt caccgttaac
      181 ccccgcagaa atcgggacag gatgtgcaaa tgtaataccg tcacccgcga cgatattccg
      241 tttgccatct ggtcagggct tcgccccgac accccgtatc agtgcgtacg tgctcaatgt
      301 ggtttccggt tctcttccgc ttcctcgccc actcgctcac taacgttcgg tcgctggggc
      361 tgcggcgagc ggtgccacct ctcgcatgtt gtcggtgatt ccgctgttgt tctttcgggg
      421 atgagtcgtc agatagccct tcaaccgcgc tcagaagctc tcagacgcac gaggcggggt
      481 ttaaatgccc gtcagggcat ggaaggcgcg aaaaacgctt acagagcgtt tcaggaggtc
      541 aggcgcgccg atacccctag tcaggggctt tcaggtcgcc aggaaccgcg ccagctccct
      601 gcaaagccca tttgcacggt acaggcagtc actcggttgg taggtggtcg tagaccgtcg
      661 gtgttacagc gatgggggtc tggggttctc cccagacggt ttccggttgc gctcaaggcg
      721 ctgccggaac cgtggcggct cggtcgttct ggagatcggg gagcaaaaac acgcaaggga
      781 ttttgaggcg gcaaaagagc aatgcaggat agacaatcat ggtcagaacg ttaacacgta
      841 gcccctgatt gtccgcctgc cgctggctca atttgaaaac atggccatga ttcggcgctg
      901 aatatcaccg tctttgttga ccagtgtttt agtgacaaat gctgcatggt ttttcgtagt
      961 gttgtatccc ttccagactt catgtggatc gagggcatac gcacaaacct taccagcaac
     1021 ggggattcta actaatgcat tttttgattc taagtaattg agccaacgaa taattgtgct
     1081 tctgctaact ccaagttttt cagctaggaa ttgctgatcc gcaacgacag cgccacacgt
     1141 aggatcgata tgctccgcta aaaatgagta cagcttcagt gctgatatgc cttggctatc
     1201 cttcagcagc tctcgaacgc gttcccaacc ttttggggat acctgtgtaa accttgaatt
     1261 cttctttgct tcttcgagat cctcggtgtt tttctttgcc tcatctgaaa gttgtttcag
     1321 tcgtttttct gaatcaataa acatgatttt ttccctctaa aaatcccatg cactaacgtt
     1381 ggttcaaatc taagccatca tgacacatct gtgcaacacc ttgttgcgtg ttgtcgcatc
     1441 caggatgcga caacgttgtg tcaccctgga tgcgacaggt aaacaaaaaa cacactaaaa
     1501 aaacaacaac ttacaaatcg gcgtaaaaga tctattctag gcaagggtgt tg
//
