LOCUS       CP063722                4910 bp    DNA     circular BCT 15-OCT-2021
DEFINITION  Escherichia coli strain 18GA07VL07-EC plasmid pVPS18EC0801-5,
            complete sequence.
ACCESSION   CP063722
VERSION     CP063722.1
DBLINK      BioProject: PRJNA292663
            BioSample: SAMN16533953
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4910)
  AUTHORS   Tate,H., Li,C., Nyirabahizi,E., Tyson,G.H., Zhao,S.,
            Rice-Trujillo,C., Jones,S.B., Ayers,S., M'ikanatha,N.M., Hanna,S.,
            Ruesch,L., Cavanaugh,M.E., Laksanalamai,P., Mingle,L.,
            Matzinger,S.R. and McDermott,P.F.
  TITLE     A National Antimicrobial Resistance Monitoring System Survey of
            Antimicrobial-Resistant Foodborne Bacteria Isolated from Retail Veal
            in the United States
  JOURNAL   J Food Prot 84 (10), 1749-1759 (2021)
   PUBMED   34015113
REFERENCE   2  (bases 1 to 4910)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2020) CVM/OR, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JUN-2020
            Assembly Method                   :: HGAP v. 4
            Assembly Name                     :: Microbial Assembly
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 506x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/27/2020 12:52:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.13
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,112
            CDSs (total)                      :: 4,991
            Genes (coding)                    :: 4,823
            CDSs (with protein)               :: 4,823
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 58 of 168
            Pseudo Genes (incomplete)         :: 103 of 168
            Pseudo Genes (internal stop)      :: 30 of 168
            Pseudo Genes (multiple problems)  :: 21 of 168
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="18GA07VL07-EC"
                     /isolation_source="Veal"
                     /db_xref="taxon:562"
                     /plasmid="pVPS18EC0801-5"
                     /geo_loc_name="USA:GA"
                     /collection_date="2018"
                     /collected_by="CVM"
     gene            503..769
                     /locus_tag="IO774_25525"
     CDS             503..769
                     /locus_tag="IO774_25525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QOX30485.1"
                     /translation="MNIVLSVSKCLYINDLDRLLPCDRFFVHLDYRDISKKESFEMNRH
                     DKETYKLIFDKLVGNDVMLSIDEDIYSEVVENLLYYSNKTYGD"
                     /name="hypothetical protein"
     gene            776..1042
                     /locus_tag="IO774_25530"
     CDS             776..1042
                     /locus_tag="IO774_25530"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QOX30486.1"
                     /translation="MIDEMTQYRIRRFTQSQKSLLLKMIKESQRSDLEDNIKDILKDID
                     VSYSTGITKKASRKEEMIACREVHYIFSILTKRGINNDYRNEI"
                     /name="hypothetical protein"
     gene            1020..1295
                     /locus_tag="IO774_25535"
     CDS             1020..1295
                     /locus_tag="IO774_25535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QOX30487.1"
                     /translation="MIIEMKYEMKLNVLARFFYYIEQDKDIPFDYSSYDEQGLCYFVAN
                     RYINENKADDLIQALIDTNDDDYIKAIRDYVQYTTLNEVRKKYEDR"
                     /name="hypothetical protein"
     gene            1282..1653
                     /gene="mobC"
                     /locus_tag="IO774_25540"
     CDS             1282..1653
                     /gene="mobC"
                     /locus_tag="IO774_25540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="QOX30488.1"
                     /translation="MKTDRKRGRIELNHEVRRDISIQFRVNKAEFEYLKELVEASGLER
                     GHYIRREILNSEPIVKRKMPDFEQSLYKEIQHQGNNINQIAKKLNTNEDFKQSVAAEAN
                     FAIKQLMKRILKACEVYTI"
                     /name="mobC"
     gene            1650..3146
                     /locus_tag="IO774_25545"
     CDS             1650..3146
                     /locus_tag="IO774_25545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="QOX30489.1"
                     /translation="MKGNVAKPGRSFKNRVDYILKDDHDFICSNMASDYNNVSDLTDEF
                     KAVSSFRLDIKKPVFHAFLSLPKNEKLTDEQWQEIAKDYLKEMNIDIDKHQYICVRHND
                     TDKEHIHIVANRVGLDGSVWLGQHSAFNTIAACERLEVKHGLTITDGLKGQKSEVSAPT
                     KNEIEQALRTGEKPARIVLQNALQAAMVGKPDLETFIDRLQAVGIEAAFNVASTGNVAG
                     VSFGIKNNEKNIYFKGSQLGKKYSWNTIKNKVIYDKNRDDELVRRFAARKTDDENSIRR
                     SPTEPDFNDSRAVDRIDDDTIGVSQHGIKPDTTDTNQSEQLVRSNHNSFKLSEKYNRPT
                     GPKVRRTTQKAAQILNRVKKQYNKEPIKTYNKNGLNSWRDKSDSGSIGRILDHAETANA
                     ATYSFRLKSGSRFNSRATKIEEIRDKLGIKKPVIQPKNKEKIVKEEIKKKPLGLSMNEN
                     RFKSKLKETLKKEKPRNETTPTQLQQEDDQHLKPRGFKPR"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3155..3427)
                     /locus_tag="IO774_25550"
     CDS             complement(3155..3427)
                     /locus_tag="IO774_25550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication protein"
                     /protein_id="QOX30482.1"
                     /translation="MKVKNRKRELTAKEYAEQYEISVRTVKRYFSQDREDYEKEAKQRR
                     LKAFVLRESGMKWAEVGIALGTTKHGAIALYKRYQQIDAPTTKEA"
                     /name="plasmid replication protein"
     gene            complement(3424..4275)
                     /locus_tag="IO774_25555"
     CDS             complement(3424..4275)
                     /locus_tag="IO774_25555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QOX30483.1"
                     /translation="MFNIENNREILKSQIPLKPYATNDLEFGLTIQNKNKALDMLYLQV
                     NHPLYLHTMIFDLDKENCFYEFENAHLPIPSFITKSPDSGRCHYGYMLNAPISSTEKSR
                     QKPVKFARALYYNMATRLGADLGYAGLITKNPLHSHWSPFWSGADLYELNDLADCFDDL
                     EDPKKRENTGLAFGRNVEMFDTIRQWAYKNVLKYQSESSFNDFHNELLLKCQMHNAYLN
                     ADDLLPYNEIKATAKSIAKFCWKEFSEEKLNKIQSKKQSYRGKKNKGIVKSKTRKFLEA
                     IK"
                     /name="replication initiation protein"
     gene            4570..4794
                     /locus_tag="IO774_25560"
     CDS             4570..4794
                     /locus_tag="IO774_25560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QOX30484.1"
                     /translation="MHEQKDKKRRLGFMIGKSNATLPADEQTEAELDQEVLKLFYPNQC
                     TKDTIERSEAGTDVFTASGSDDLFKKLKL"
                     /name="hypothetical protein"
ORIGIN
        1 tttatagcca tttcaaaacc atagacgcca gcaaacgagc aaaagcaagt tttactcgtt
       61 tctgtcgccg ccgatcaaac cccattcctt aaaatctgcc taccaagtgg tcacgaactt
      121 ttccagattt cacgtagcag cggcattttt tacgcaaagc tacttattac gcacagctac
      181 ccatcaagac aatatattat ttaccttatt tttatttgca ttttatttgt cgtgcagtgt
      241 atttatttat gtagccgcta tattgacaaa ctttttagat cggttattta tttaatatag
      301 gttgagttca gctttatttt ttagcttttc ggaaactgag gcaaggtgta atcccgtagc
      361 cggtattata cccaaccttt caggttgccc cctctcactt cgttcggagg gttgttaaaa
      421 gatctaccct tccgcctatt ccccttatca aaggggaggc ggtaaatagt tttacatcac
      481 ttcaaaacaa aaaggaataa atatgaatat agttttatca gtatcaaaat gtttatatat
      541 aaacgattta gatcgcttac ttccgtgtga tcgttttttt gttcatttag attatagaga
      601 tattagcaaa aaagagtcat ttgagatgaa tagacacgac aaagaaacat acaaacttat
      661 ctttgataaa cttgtaggta atgacgtaat gctttctatt gatgaagaca tctacagcga
      721 agttgtcgaa aacttacttt attattcaaa taagacttat ggagattaat ataaaatgat
      781 agatgaaatg acacaataca gaataagacg ctttacacaa agtcaaaaaa gcttgctact
      841 aaagatgata aaagaaagcc agcgaagcga tttagaagat aatataaaag atattttaaa
      901 agatattgat gttagttatt caactggcat aacaaagaaa gcatcaagaa aagaagaaat
      961 gatcgcttgt cgtgaagtcc attacatctt tagtatatta acaaaaagag gcattaacaa
     1021 tgattataga aatgaaatat gaaatgaaac ttaatgtatt agcacgtttt ttctattaca
     1081 tagaacaaga taaagatata ccttttgatt actcaagcta tgacgaacaa ggattatgtt
     1141 attttgtagc taatagatac attaacgaaa ataaagctga tgatcttata caggcattga
     1201 tagatactaa tgacgatgat tatataaaag ctataagaga ttatgttcag tatacaactt
     1261 taaatgaagt gagaaagaaa tatgaagaca gatagaaaaa gaggacgtat agagttaaat
     1321 cacgaagtac gtagagatat atctatacag tttagagtaa acaaagcaga gtttgaatat
     1381 ttaaaagaac ttgttgaggc gtctggcctt gaaagaggtc attacattag aagagagatt
     1441 ttaaactctg aacctattgt taaaagaaaa atgccggatt ttgagcaatc attgtataaa
     1501 gaaatacaac atcaaggtaa taatataaat cagattgcta aaaagctaaa tacgaatgaa
     1561 gactttaaac aatctgtagc cgcagaagct aactttgcta taaagcaact tatgaaaaga
     1621 atattaaagg cttgtgaggt ttataccata tgaaaggaaa tgtagcaaaa cccggaagat
     1681 catttaaaaa tagagttgat tacatattaa aagatgatca cgatttcata tgtagcaaca
     1741 tggcaagtga ttataataat gttagtgatt taactgatga gttcaaagct gtaagcagct
     1801 ttagacttga tataaaaaaa ccagtctttc atgcgttttt atctcttcca aaaaatgaga
     1861 agctcacaga tgaacaatgg caagaaatag caaaagacta tttgaaagaa atgaatattg
     1921 atatagacaa acatcaatat atttgtgttc gtcataacga tacagataaa gaacatatcc
     1981 atattgtagc taatcgtgtt ggccttgatg gttctgtatg gctcggtcag cactcagcat
     2041 ttaacacaat agcagcttgt gaacgtttag aagtaaaaca cggcttaacc atcacagatg
     2101 gcttaaaagg tcaaaaatca gaggtatcag caccaaccaa gaacgagatc gagcaagcct
     2161 tgaggacagg tgagaaacca gctcgtatcg tgctacagaa cgcattacag gcggctatgg
     2221 taggaaaacc agatcttgag acattcatag acagactgca ggctgtaggc atcgaggcgg
     2281 cctttaacgt tgcaagcact ggcaacgtcg ccggggtgtc gtttggtatc aaaaataatg
     2341 aaaaaaatat ttatttcaag ggatctcagt tgggtaaaaa atacagttgg aacactataa
     2401 aaaacaaggt gatatatgac aaaaatagag atgatgaact tgttagaaga ttcgcagcaa
     2461 gaaaaacaga tgacgaaaat agtattagac gatcacctac tgaaccagat ttcaatgaca
     2521 gtagagccgt tgacagaatc gatgatgata ctatcggagt cagtcagcac ggcattaaac
     2581 cagacacaac agacactaac cagagtgaac aactcgttag aagcaaccac aacagcttta
     2641 aactcagcga aaaatataat agaccaacag gcccaaaagt tagaagaaca acacaaaaag
     2701 ctgcacaaat actcaacaga gttaaaaaac aatacaataa agaaccaatc aaaacttata
     2761 ataaaaatgg cctcaatagt tggcgtgata aatctgatag cggttctatt ggtaggatac
     2821 ttgatcatgc agaaacagca aacgcagcca cctattcatt tagactcaag agcggtagcc
     2881 gtttcaatag tcgagcaact aagatcgaag agataagaga taaacttgga ataaaaaaac
     2941 ctgttattca gccaaagaat aaagaaaaaa tagttaaaga agaaataaag aaaaaacctt
     3001 taggtttgag tatgaatgaa aatagattta aatcgaagtt aaaagaaacc cttaaaaaag
     3061 aaaaaccaag aaatgaaaca acaccaacac aactacagca ggaagacgat cagcatttaa
     3121 aaccaagagg ttttaaaccg cgataaaaga ggctttaagc ctcttttgtt gttggtgcgt
     3181 ctatttgttg ataacgttta taaagagcta tagcaccatg ttttgtagtt ccaagagcaa
     3241 taccaacctc tgcccatttc atcccgcttt cacgtaatac aaaggctttt aatcgtcttt
     3301 gttttgcttc tttctcatag tcttcacgat cttgagagaa atatcttttt actgttctaa
     3361 cgctaatctc atactgttca gcatactctt ttgcagtaag ttctcttttt ctatttttaa
     3421 ctttcatttt atggcctcca aaaacttacg agttttactt tttactattc ctttgttttt
     3481 ctttccacgg taagactgtt ttttactctg tatcttgttg agtttttctt cgctgaattc
     3541 tttccaacaa aactttgcaa tgctttttgc tgtggctttt atctcattat atggcaataa
     3601 atcatcagca tttaaatatg cattatgcat ttgacacttt agcaatagtt cattgtggaa
     3661 atcattaaat gagctttcac tctgatattt tagcacattt ttataagccc actgccttat
     3721 tgtgtcgaac atttcaacat tacgaccaaa agcaagcccc gtattttctc gttttttggg
     3781 gtcttctaaa tcatcaaaac aatcagctaa atcattaagt tcgtataaat cagcaccaga
     3841 ccagaacggc gaccaatggc tatgcagtgg gtttttagta atcagtcccg catagccaag
     3901 atcagcacca agacgagtag ccatattgta atataatgct ctggcaaact tgacaggctt
     3961 ctgtctgctt ttctctgttg atgagatcgg agcattaagc atataaccgt aatgacatct
     4021 accagagtct ggtgattttg ttataaaaga cgggatgggc aaatgagcat tttcaaactc
     4081 ataaaagcag ttttctttgt ctaaatcaaa tatcattgta tgaagataca acgggtgatt
     4141 tacttgtaaa taaagcatat ctaaagcttt attcttattt tgaatagtca atccaaactc
     4201 taaatcattt gtagcatatg gctttaaagg tatttgactt tttaatatct ctctgttatt
     4261 ttctatattg aacatttagc agtatccttt ctgtaatgcc ccgttctctc actgtcgcca
     4321 aactaatcag agaacgcggg cttttctttt atttataatc tcttacagtt ttactatacc
     4381 aaaaaaactt gtcaatagat ctaccaaaaa aagttgttat gtgtgccatt aaccaatatc
     4441 aggtattaga cggagttttg gggatactac accacagcga agcacaggcc gaaggctact
     4501 gcagagtaag tcacactgta acgtatacta aactctatgc aggatctggc tgtgctgggg
     4561 aggtacatca tgcacgaaca gaaggacaag aaacggcggt taggttttat gattggtaag
     4621 agcaatgcaa cattgccagc agacgaacaa acagaagcag agcttgatca ggaagtcttg
     4681 aagctgtttt atcctaacca atgcaccaaa gacaccatag aacgctcaga agctggtaca
     4741 gacgttttca cggcctccgg ttctgatgac ctgttcaaaa aattgaagct gtagagggta
     4801 ggggagagct ggccatcttc accacttgag aacaaaccga cccggaacgg tgagagaagg
     4861 ccaaccctcg atgcagtata tactgattta tactaatcta tagtgttgat
//
