LOCUS       CP063716                5167 bp    DNA     circular BCT 15-OCT-2021
DEFINITION  Escherichia coli strain 18GA07VL08-EC plasmid pVPS18EC0802-3,
            complete sequence.
ACCESSION   CP063716
VERSION     CP063716.1
DBLINK      BioProject: PRJNA292663
            BioSample: SAMN16533954
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Tate,H., Li,C., Nyirabahizi,E., Tyson,G.H., Zhao,S.,
            Rice-Trujillo,C., Jones,S.B., Ayers,S., M'ikanatha,N.M., Hanna,S.,
            Ruesch,L., Cavanaugh,M.E., Laksanalamai,P., Mingle,L.,
            Matzinger,S.R. and McDermott,P.F.
  TITLE     A National Antimicrobial Resistance Monitoring System Survey of
            Antimicrobial-Resistant Foodborne Bacteria Isolated from Retail Veal
            in the United States
  JOURNAL   J Food Prot 84 (10), 1749-1759 (2021)
   PUBMED   34015113
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2020) CVM/OR, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JUN-2020
            Assembly Method                   :: HGAP v. 4
            Assembly Name                     :: Microbial Assembly
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 312x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/27/2020 13:01:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.13
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,066
            CDSs (total)                      :: 4,947
            Genes (coding)                    :: 4,741
            CDSs (with protein)               :: 4,741
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 206
            CDSs (without protein)            :: 206
            Pseudo Genes (ambiguous residues) :: 0 of 206
            Pseudo Genes (frameshifted)       :: 95 of 206
            Pseudo Genes (incomplete)         :: 108 of 206
            Pseudo Genes (internal stop)      :: 49 of 206
            Pseudo Genes (multiple problems)  :: 37 of 206
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="18GA07VL08-EC"
                     /isolation_source="Veal"
                     /db_xref="taxon:562"
                     /plasmid="pVPS18EC0802-3"
                     /geo_loc_name="USA:GA"
                     /collection_date="2018"
                     /collected_by="CVM"
     gene            complement(join(4688..5167,1..54))
                     /locus_tag="IO775_25310"
     CDS             complement(join(4688..5167,1..54))
                     /locus_tag="IO775_25310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QOX25663.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISLDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTMLDRNYNATAAVYQKVVTLED
                     KLAEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(57..983)
                     /locus_tag="IO775_25315"
     CDS             complement(57..983)
                     /locus_tag="IO775_25315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QOX25664.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPDRGGAKKDSGNLT
                     PTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYYRHLEQARMVGHDIREWLDTPVKQHELVRRLQHDIAREHGFD
                     MRDGVSRQNRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            1160..1558
                     /locus_tag="IO775_25320"
     CDS             1160..1558
                     /locus_tag="IO775_25320"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QOX25665.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            complement(1762..2676)
                     /locus_tag="IO775_25325"
     CDS             complement(1762..2676)
                     /locus_tag="IO775_25325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QOX25666.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            4413..4622
                     /locus_tag="IO775_25330"
     CDS             4413..4622
                     /locus_tag="IO775_25330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QOX25662.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLRKLPPDHTIPPDLTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     Typing          complement(1902..2676)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.907"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          complement(61..983)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cagatgctcc accgataaca ggagcgtgag taatttttct tctgtgctgg ccatgttcag
       61 aatcccatag aaaatccacg tgacggtgat ttaacccgtt caggttcagt tcggttctgt
      121 ctgctcactc cgtctctcat gtcaaagcca tgttcccgcg caatatcatg ttgcagcctt
      181 ctgaccagtt catgttgctt aaccggtgta tccagccatt ccctgatgtc atgcccgacc
      241 attcgcgcct gttcaagatg gcggtaatag tccggttcag accgtttttg catgatgtca
      301 tgacgctggg catcatcaag tcgtccggca gcttcaaaac ccagatgcac ttcgggacgt
      361 cgttcaatgc cctgttcttt cagcgtccgg cagtcaatga agcccctttc aaagccatgc
      421 tttctcatat gttcgttgtg cttcacctcc cagcgttttc gcaattcccg caactgctct
      481 ttttgctgag tgggggtcag gtttccggaa tcctttttcg cacctccccg gtcgggatac
      541 ctggcgttat agcgccggaa atactgttcc ggtgtacgtt caatgccatc attcacacgc
      601 tgactcatca tgatatgtgc atgaggctgt tcgccgccgt caatgctcgc tttcggattg
      661 tgaatggcaa aactccaggc gtgccgttcg ccagcttcct gacgtacaaa atcctgcacc
      721 agttcaaggc gctgttccgg tgtcagttca cggggtaatg caatttcaag ttccctatag
      781 gtcgaaccgt tcgcacgttc aaactggtca gccgcctgcc agaaatggga aggattatcc
      841 ctggcccatt ccggcatgtt tccgtgtgcg gaatattcca aatcttgacg gtcgcggtat
      901 ttctctttgc gctcgatgta atcggcatgg ttggcagcct gtcccttgcc accaaattta
      961 acgctgaggt gataggtcgc cacggtttca gccctccgca ggagcgcact gtttctcaac
     1021 gaaggagaga aacatataag tgcgcttcct tctaatttta cgaattagca cggaagatac
     1081 cccctgcacc cgccgaccgc aagcgatata ctgcacgacc acaacacacc gcaaaaaaac
     1141 accagggagc atcacgaaca tgcgcatcag caacatcgaa tggctgaaga aacggatcgg
     1201 attcatcagg aagctcggag aacagaccgc acgacagaga cagattattg acctgatcga
     1261 caacgaagcc ggactcacag aacaggaacg caaacttctc cacgtactgg caacagcgga
     1321 aaaaaacgac ttacaggcac aggagagcga acgaaaacag gcggtccaaa aacgaatcga
     1381 gggaaaaaaa caacgacggg aacgcaatca ccggctcttt ctcgcggctg ggctactgat
     1441 cgaagcagga ctggtagaca caaaaacagg cgagctatgc tataaaaaag acaggatatt
     1501 acaagcactt aaggaactta agtacgatct ggagacatcc ccaaacccag acgcgtaagt
     1561 gcctccatcc gtgttccgat tccctggtgc ttcctcgctc attcgctcgc tacgctcggt
     1621 catgagactg cggcgagcac taccaccaca atcacgcgga aagccgcgcc attgcagggc
     1681 tgaacgggga aaaacggcaa aaaacctgct gctcaaaaag acgccagaaa ggcgctgtta
     1741 tctgatatag gcttatttgg tctactctac gagacctcgt tttttcagtt ggtaatacca
     1801 ggcgcgactg atccctaatg cctcccacgg tttcagcgtg cgtgcagatg tagctactgt
     1861 gctgcgctta gacttagaac cgcccttgcg accgcgtgca gcctgaattt ctggcgtgtg
     1921 cgtatcagcc acatactgtg cgaaagtttc cggcgtgaag ttcctgtgcg tgtatttcgc
     1981 aatgctcttg ccaatagccc gacattcagc cagtgaaagc ggaacgggaa gcgatgcatt
     2041 gtacatttcg acacgctgga tcaccgcttc aagccactgt gagaacgctg gccagccctg
     2101 acgaatcgcc ctgtaagccc atttgcgcgt catttcgaac aggtggcagt ttcgccccat
     2161 cccgtaatgt ttatcgatgc tacgacgcgc tgaggcgctc aaatcgagat aatcagccag
     2221 ttcatcgagg gtataggctt cctcgcgcca ctccatcacc agccattcca ggtggaacgg
     2281 atttttgcaa atcaggccgc tgtagttcac atccgcacaa agtttctcac acagcgaacg
     2341 ttcaaccgct gcggcatatt tcagcgcctt aaccgaggca tcaggcgcgg ttctcacggc
     2401 gatgttgagt gcgtagagca ggtgagcgtg tccgttcaca ggatttttaa cggtgatgtt
     2461 cggcgcggga gcgttccggt cgctccagtc aatcgcagcc ccctcgcggt caacgtcaaa
     2521 aaccagccag aactgcgcat gaggctggtt ctgctgtatg taccgcgcaa gaagcgcacg
     2581 ccctttgccg gagatgcgaa gaccaaaagc gagatcatcc gtgtgatagg gacgatgggg
     2641 taaattttcg tcgaagtatt gaagcgcggc gctcatgtgt tacgattctc tccgggttat
     2701 gcgacctaca cgccctctgt atttcatgtt gaatcttgcc ggatctggac atgatttcag
     2761 gggcgtttgt ttttcagtcg cttacactac acccaacggc aaatcctgtc gaaccttccc
     2821 gaaaacccga tccatttacc ataactcccg ttgtacgtac ccattaaaac gccctcaatc
     2881 gctttctggc gcaaaaaacg caaaaagaca agaaaacacc aggccaaacc agaaaaacgc
     2941 gtcagaatcg ttttcagggc gttgcataac tatgcacgaa agcgatgtgc gatcggtttc
     3001 gtgcgttctg aacaccgaac ggctcaaaac gacaagtttt ctcccctggt gcgtggcgct
     3061 ctgacggggt ttcctgtttc caaacttcac atctcgccag gacaaaaaaa aaccgacctg
     3121 gggtcggtgg caagaatcag agtctatggg aaatatttta gttatcggat gctcaatatt
     3181 cacaaacgca aaaaatgcct cagtgatgcc ctaaaacaac ctttccagaa ggagtccgcg
     3241 ctttacgatg cacggaagag ataaaaaacc ccacaaaagt ggggaaccaa gagaaacaac
     3301 aattacaggt tgttggttgt gttcgaacaa cgatgaagta gctacgctaa ataataatta
     3361 ccgtacaggc cctacatatt caacactaaa tttaagatcc tgacgaagga tttataaatg
     3421 cacgaaaaca catagacaga caaattcact aaattcatac acaaaaacaa gtgtagtttt
     3481 ctaagtaaat tagattcgac aaccaattag tttatccaca tcgcattcca ctcttgctac
     3541 accaatcaca tttcttgata tgtaatagca gaaaaaataa aaccagacac caatagacca
     3601 aaaaacaaac attcaacaag aaacattttg aacaatatca caaaactgtc acaaccatca
     3661 tcagactatt gaaaaaaact ggttgctgtt ttataaaagc gactgtaaca acctccactc
     3721 tatagttcca ttagtttaaa gtgttacaca aactgagcat ccatttgttt tccttacttt
     3781 tgcccctgtg ttccaagggg tttttttata acttaaagat tagcacacca cacaaaaaac
     3841 aataaaaaac aacaagttat acaataaaaa cagtactatc catacagcta attacatata
     3901 aataccatta aaccatctgg acattaaaaa taaactctta taacataaaa ttaatgcggt
     3961 tccggtacca tatgtcaggg ataatgtgca aatggaggta tcggaactgc atttcagttc
     4021 attattaatt aatgacaaaa aaaaaccctc tgagagcgca gagggggtaa ggaatgttcc
     4081 cggtcataat atgtcgatag tgtttatata catacagaac aagaacaaca accaaccaac
     4141 taataaatat tttatttttt agagctgaaa ccttaaagct cacttccatt ccatacattg
     4201 ttataaacct tatgttgaaa actgaaagta agtacggcag acttattaag ttgcgtgcag
     4261 tcttttcacg ttatacaaac aagaacacct gcgaagttat ttctccttac atatccccct
     4321 atgccaacgg gggatttttt ctggtttact aacgcataaa taatagtgac cccctatcat
     4381 tcacgaaccg tgataatata aggattatca tcatggcaaa attaggtgaa aacaccccac
     4441 atattatcga tgctactgta gattttatgg catcaagcca ggcatttcgt gagtatctga
     4501 gaaaacttcc ccctgatcac acaataccgc cagatcttac tgatgagcac gtaccgttat
     4561 atatacgaag gctggaatac tatcgactgc tttatcgacc aaagcagaca gaatcatatt
     4621 gaaaaaaacg gtgaacggtt cgcgaaatcc tttcgcgctc cgtgaggcgt tggacaagcg
     4681 aagcgcgtca gtttttccgg ctcttacccg tgacaggttt ctccgccagt ttatcttcca
     4741 gtgtcacaac tttctgatac accgccgctg tggcgttgta gttcctgtcg agcattgttt
     4801 tcattcccca tccgagcaca cagaacataa ccagaaaagc cactgacaca ccacccagcc
     4861 atttccagct atacccttta agggtgttgt attcctgcac gatattgctg tgaattgtcg
     4921 caatatccct gttcagggca tccgcgtttg ccttcatgtt tgcgactgac tcttccagcc
     4981 gctgaaaatc caggctgatg cgctcaccta tcagccctgc tacgtccagc ttctgcacct
     5041 catttctgac ttcatccctg acctgttttg ccacaccgtc agaaatatcc cgatatccgc
     5101 gaagcgtttc ccttgccaca gccagagaat cagcaacagc tttctgttgc tccattgcgg
     5161 tatgttg
//
