LOCUS       CP063714                6715 bp    DNA     circular BCT 15-OCT-2021
DEFINITION  Escherichia coli strain 18GA07VL08-EC plasmid pVPS18EC0802-2,
            complete sequence.
ACCESSION   CP063714
VERSION     CP063714.1
DBLINK      BioProject: PRJNA292663
            BioSample: SAMN16533954
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6715)
  AUTHORS   Tate,H., Li,C., Nyirabahizi,E., Tyson,G.H., Zhao,S.,
            Rice-Trujillo,C., Jones,S.B., Ayers,S., M'ikanatha,N.M., Hanna,S.,
            Ruesch,L., Cavanaugh,M.E., Laksanalamai,P., Mingle,L.,
            Matzinger,S.R. and McDermott,P.F.
  TITLE     A National Antimicrobial Resistance Monitoring System Survey of
            Antimicrobial-Resistant Foodborne Bacteria Isolated from Retail Veal
            in the United States
  JOURNAL   J Food Prot 84 (10), 1749-1759 (2021)
   PUBMED   34015113
REFERENCE   2  (bases 1 to 6715)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2020) CVM/OR, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JUN-2020
            Assembly Method                   :: HGAP v. 4
            Assembly Name                     :: Microbial Assembly
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 312x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/27/2020 13:01:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.13
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,066
            CDSs (total)                      :: 4,947
            Genes (coding)                    :: 4,741
            CDSs (with protein)               :: 4,741
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 206
            CDSs (without protein)            :: 206
            Pseudo Genes (ambiguous residues) :: 0 of 206
            Pseudo Genes (frameshifted)       :: 95 of 206
            Pseudo Genes (incomplete)         :: 108 of 206
            Pseudo Genes (internal stop)      :: 49 of 206
            Pseudo Genes (multiple problems)  :: 37 of 206
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="18GA07VL08-EC"
                     /isolation_source="Veal"
                     /db_xref="taxon:562"
                     /plasmid="pVPS18EC0802-2"
                     /geo_loc_name="USA:GA"
                     /collection_date="2018"
                     /collected_by="CVM"
     gene            192..575
                     /locus_tag="IO775_24655"
     CDS             192..575
                     /locus_tag="IO775_24655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QOX25549.1"
                     /translation="MSNIVHLSDKKQKNKPVDISFDDYTDNLSKGFVKNLSNKQKKELI
                     SKIKAILKSRNVAFRSTPPSDWKEKSRTFAIFFPEKKIVFTLGVVNKLKIEDGWINHKI
                     SDTEDAVLSVGKIINLIEDSQHG"
                     /name="hypothetical protein"
     gene            568..774
                     /locus_tag="IO775_24660"
     CDS             568..774
                     /locus_tag="IO775_24660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QOX25550.1"
                     /translation="MAKLPQEKPKEVIQAGFDDVKKAYKKGAKTVNDTYAEQAGRDLQS
                     GDGKLMATYRRNKKAIDKLDAEG"
                     /name="hypothetical protein"
     gene            complement(1149..1382)
                     /gene="mbeD"
                     /locus_tag="IO775_24665"
     CDS             complement(1149..1382)
                     /gene="mbeD"
                     /locus_tag="IO775_24665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QOX25551.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSERTERLSQLYSENWR"
                     /name="mbeD"
     gene            complement(1389..1907)
                     /locus_tag="IO775_24670"
     CDS             complement(1389..1907)
                     /locus_tag="IO775_24670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QOX25552.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGMSAEVTHYQ
                     GKDWIQLTE"
                     /name="MbeB family mobilization protein"
     gene            complement(1885..2595)
                     /locus_tag="IO775_24675"
                     /pseudo=""
     CDS             complement(1885..2595)
                     /locus_tag="IO775_24675"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2585..2908)
                     /locus_tag="IO775_24680"
     CDS             complement(2585..2908)
                     /locus_tag="IO775_24680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QOX25554.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIEGELRSLRQVVREQGAR
                     DDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2973..3164
                     /locus_tag="IO775_24685"
     CDS             2973..3164
                     /locus_tag="IO775_24685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QOX25553.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGEDL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4210..5367)
                     /locus_tag="IO775_24690"
     CDS             complement(4210..5367)
                     /locus_tag="IO775_24690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="QOX25547.1"
                     /translation="MRIIDLFSGCGGLSLGFLKGGFDVVGAYDFWDPAIECYRDNFSHP
                     IKKLDLSNVDDVVRELKDIDFDMIIGGPPCQDFSHAGLRIEGARANLTRSFSEIIKRIK
                     PKWFVMENVDRALRSGAYLEARGIFKESGYGLTEIVLDASKCGVPQKRKRLFVIGKLDV
                     RDGFILNEVMCGISKDSMTVRNYLGDSLGIEYYYRHPRNYNRRAIFSIDEPAPTVRGVN
                     RPIPDGYLGHAGDPVSISENVRPLTTFERARLQTFPEDFKFKGAKTNLEQMIGNAVPVE
                     LAKYVAVTIMEYEKKQVKGIYDKEGFRAWLLNEKKLTKRTSSDIISRCCRGVSFFDSEG
                     VDFYNCEIDEIIMKLERLESFVRLGVSLKSQLRRAFKLYYEYCRR"
                     /name="DNA cytosine methyltransferase"
     gene            5455..6516
                     /locus_tag="IO775_24695"
     CDS             5455..6516
                     /locus_tag="IO775_24695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HindVP family restriction endonuclease"
                     /protein_id="QOX25548.1"
                     /translation="MSKIESLYGLKNTNRKESDLWGKNQFNSSFPTSLACYMRDNKMNP
                     IYLYLDKDLKVHAKEVSFDFVFNTTLKNSDLEFQFEKKYEPYQQYAYDDIKGIDLVIKG
                     NNSFLKALEIKLTVLPDSTTYKENESEWSSEIVIRPASTSYCALGIMHSCSDKFHEIRK
                     IFESTCESIQHWGNETEVLSKKENILNCLNTFQENFLDYQKPFLMQPLWKTKGKSPILD
                     QNAFDIFIWSDFALCRTFIDRSSYNNSSASRLMRSSARLARMVYEIASKNKTNINRIYT
                     EMNFGYQSDKEFSLNGKITKAYMDHPRRTKPILPPSVLKEIILNGGQNKLSPERRFDQS
                     VYYTASHLFDEIK"
                     /name="HindVP family restriction endonuclease"
     Typing          complement(3243..3327)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.674"
                     /coverage="100.0"
                     /evalue="6.569999999999999e-34"
     Typing          3347..3893
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.455"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(1052..2595)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.529"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2592..2941)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.162"
                     /coverage="99.0"
                     /evalue="1.96e-64"
ORIGIN
        1 ctggttgctt catgcccgta aaggcaattt cgacggtcat tacattgaac ataacatctc
       61 gaccgcgtac tgaacggtct cagccggatc ttcgaaccca tctatcgtag aattgatact
      121 ctacctctgt gactaacaag acagttccgc tactcttctg agtattcatt catcaaaaag
      181 caggatatcc agtgtcaaac attgttcatc tatctgataa aaaacaaaaa aacaaaccag
      241 ttgatatttc ttttgatgat tacacagaca atctcagcaa agggttcgtc aaaaatctca
      301 gcaataaaca aaaaaaagaa ttaattagta aaatcaaagc gatacttaaa tcgaggaatg
      361 tagcgttccg ttcgacgccg ccctctgatt ggaaagagaa aagtagaacc tttgcaattt
      421 tctttcctga aaaaaagatt gtttttacat taggagtagt gaacaaatta aagatagagg
      481 acggttggat aaatcataaa atcagcgaca cagaagatgc cgtcttatct gttggaaaaa
      541 taatcaacct tatagaggat tcacagcatg gctaaattac ctcaagaaaa gccgaaagaa
      601 gtcatccaag ccggatttga tgatgttaaa aaggcctata aaaaaggcgc taagactgta
      661 aacgatacct atgctgagca agcaggtcgt gacttacaat caggcgatgg taaactaatg
      721 gctacctatc ggcgcaataa aaaagcgata gacaagttag atgctgaagg ttgatctgaa
      781 aagtcagtct tctgggcgga cttttcgtct ctaagactgg ctgcatattg tatttaccat
      841 aacatccgtt acgcacaccg ctaaaacgcg ttcggtgcga tcacggcagc agacaggtaa
      901 aaatggcaac aaaccacccg aaaaactgcc gcgatcgcgc ctgataaatt ttaaccgcat
      961 gaatacctat gcaaccagat ggtgcagact acattagtcc gtcaaaatcc actgaacctg
     1021 ccatttttta tagtttcgcc atcacagcga caacgaagga ccatccagta tgcgttcctg
     1081 tatttccggc tgacgctccc gttccaggga taacgcatgt tcgcgctcct gtatcagccg
     1141 ttcctctctt atctccagtt ctcgctgtat aactggctca agcgttctgt ccgctcgctc
     1201 aagtgctgca cctgctgact caactgcatg acccgctcgt tcagcatcgc gttgtcccgt
     1261 tgcgtaagcg aaaacatctt ctgcaattcc acgaaggcgc tctcccattc gttcagccgc
     1321 tgcatatagt cctgttgcag ctgctctaag gcgttcagca aatgtgtttc cagctctgtc
     1381 attccgtatc actccgtcag ttgtatccag tccttcccct gatagtgcgt cacctctgct
     1441 gacatccctt gcggaatgac cagaaatttc cttctcccgt cctgcacaaa ctccacgccc
     1501 catgtcttcg cgttcagttt ctgcaatgtc tcttcctgct tcctgatttc ttccaggttc
     1561 gcctgtatcc tcccgccaag ataccagagc gtcccgccac tcgcggtaaa caggagaaag
     1621 actatcccca gtaacatcat gcccgtattc cctgccagct ttaacacgtc cttcctgtgc
     1681 tgcatcatcg cctctttcac cccttccctg tgtttttcca gcgatttctc tgtcgaggct
     1741 gtgaacaggg ctatagcgtc tctgattttc gtctcgtttg aggtcacagc ctcgctgaca
     1801 gactcgccga gcttcctgaa ctcgtcgttc agcattttct ctgtagattc ggctctctct
     1861 ttcagctttt tctcgaactc cgcgcccgtc tgtaaaagat tgctcataaa acgctccttt
     1921 cagcctgata ttcttcccgc cgttcgggtc tgcaatgctg atgctgcttc gcgtcaccct
     1981 gaccacttca agccccgcct ctgtgagcgc ctgaatcaca tcctgacggc cttttatctc
     2041 ccctacatga taaagagcgt ctatcccgcg tgtgacgctc tcagcaagcg cctgtttcgt
     2101 ttccggcagg ttatcagaga gagtcagtat ccggcggttc tccggggcgt tggggtcatg
     2161 cagcccgtaa tggtgattca ccagtgtctg ccaggcatca attctcggcc tgtctgctcg
     2221 gtcgtagtac ggctggaggc gttttccggt ctgtagctcc atgttcggaa tgacaaaatt
     2281 cagctcaaga cgccccttgt cctggtgctc cacccacaga atgctgtact gatttttttc
     2341 gagaccgggc atcagtacgc gctcaaagct ctccatcact ttttcacgtc ctcccggcgg
     2401 cagttccttc tccgcgaacg acagaacacc ggacgtgtat ttcttcgcaa atggcgtggc
     2461 atcgatgagt tccctgactt cttccggatt accctgaagc accgttgccc cttcccggtt
     2521 acgctccctt cccagcaggt aatcaaccgg accactgcca ccgccttttc ccctggcatg
     2581 aaatttaaca atcatcccgc gctccctgct ccctgacgac ctgccgtaag ctgcgcaact
     2641 ctccctcgat ggccatcagt gcggccacca cctgaacccg gtcaccggaa gaccactgcc
     2701 cgctgtttac cttcctcgct gtctgattca ggttattccc gatggcggcc agctgacgca
     2761 gtaacggcgg tgccagtgtc ggcagcttcc cggaacgggc aaccggctca cccaggcaga
     2821 cccgccgcat ccataccgcc agttgtttac cctcacagcg ttccagtaac cgggcatgtt
     2881 catcatcagt aacccgtatc gtgagcatcc tctcgcgttt catcggtatc attaccccca
     2941 tgaacagaaa tcccccttac acggaggcat cagtgaccaa acaggaaaaa accgccctta
     3001 acatggcccg ctttatcaga agccagacat taacgctgct ggagaaactc aacgaactgg
     3061 acgcagatga acaggccgat atttgtgaat ctcttcacga ccacgccgat gagctttacc
     3121 gcagctgcct cgcgcgtttc ggtgatgacg gtgaagacct ctgacacatg cagctcccgg
     3181 agacggtcac agcttgcctg tgagcggatg ccgggagcag acaagcccgt cagggcgcgt
     3241 cagggggttt cggggcgaag ccctgaacca gtcacgtagc gatagcggag tgtatactgg
     3301 cttaactatg tggcatcagt gcggattata agaaaagagc accatatgcg gtgtgaaata
     3361 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
     3421 gactcgctac gctcggtcgt tcggctgcgg cgagcggagt cggctcactc aaaggcggta
     3481 atacggttat ccacagaatc aggggataag gccggaaaga acatgtgagc aaaaaggcaa
     3541 aggacaacaa aaggccgcgc cgctggcgtt tttccatagg ctccgccccc ctgacgagca
     3601 tcacagaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     3661 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     3721 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgttg
     3781 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     3841 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggaaagaca
     3901 cgacttaacg ccactggcag cagccactgg taactggaca aggtggattg agatattaag
     3961 agttcttgaa gtggtggcct aactgcggct acactagaag gacagtattt ggtatctgcg
     4021 ctccaccaag ccagttacct tcggaagaaa gagttggtag ctctagattc cggcaaacaa
     4081 accaccgttg gtagcggtgg tttttttgtt tacaggcagc agattacgcg cagaaaaaaa
     4141 ggatcacaag aggatccttt gatcttttta tcccctcccc ctcccctgag agacataaat
     4201 tcgtatttat taccttcgac aatattcgta atataattta aaagcccttc ttaactgaga
     4261 cttcaggcta accccaagcc tgacaaacga ttcaagcctt tccagtttca taataatttc
     4321 atctatttca cagttataaa aatccacccc ctctgaatca aaaaatgaaa cacctctgca
     4381 acatctagat ataatatcgc tagaggttct ttttgttagt ttcttttcat tcagcaacca
     4441 cgccctaaag ccttctttat cataaatacc ttttacttgt tttttttcat actccatgat
     4501 tgtcaccgca acatattttg ctaactcaac cggaacagca ttacctatca tttgctccaa
     4561 atttgttttt gcacctttaa atttaaagtc ttcaggaaat gtttgcaatc ttgctctttc
     4621 aaaggttgtt aatggcctta cattctcaga aatactgaca ggatcgcctg catgccctaa
     4681 atatccatca ggtatcggtc tattaacccc tcttactgtt ggcgcaggtt catcaataga
     4741 gaaaatagcc cttctgttat aatttctcgg atgtctgtaa taatactcaa tccccaatga
     4801 atcaccaaga taatttctaa ctgtcatact atctttcgat atcccgcaca tcacttcgtt
     4861 aaggataaaa ccatccctca cgtcaagctt tccaattaca aataatcttt ttcttttttg
     4921 cggaacacca cacttactgg catccaaaac aatttctgtt aacccatacc ctgattcttt
     4981 aaagattcct cgagcttcta aataggcacc acttcttaat gcccgatcta cattttccat
     5041 cacaaaccac tttggcttta ttctttttat aatttctgaa aatgagcgag ttaagttagc
     5101 tctagcacct tcaattctta aacctgcatg actgaagtct tggcaaggag gccctccaat
     5161 aatcatatcg aagtctatat ctttaagctc cctcaccaca tcatcaacat tgctcaaatc
     5221 tagttttttt atcggatggc taaaattatc tctgtaacat tcaatagcag gatcccagaa
     5281 atcataagca cctacaacat caaacccccc cttcaaaaag cctagggaca accccccaca
     5341 cccactaaaa agatcaatta ttctcataac ataaaaaact cattatcata tcactccaca
     5401 tcatgatata gaaacacacc atagacgtca ataaattgac gtcaggagca aaaagtgagc
     5461 aaaatagaat ctctttatgg tttaaaaaac accaacagga aagagagcga tctgtgggga
     5521 aagaaccaat ttaacagttc ttttcccacc tctctagcat gttatatgag agacaacaaa
     5581 atgaatccga tttatcttta tttagataag gatttaaaag tgcatgccaa agaagtatct
     5641 tttgacttcg tttttaacac aacattaaaa aatagtgatc ttgaatttca atttgagaaa
     5701 aaatatgaac cataccaaca gtatgcttat gatgacataa aaggaataga tctagttata
     5761 aaaggaaaca atagtttttt aaaagcacta gaaataaaat taaccgtact tccagatagc
     5821 acgacatata aagaaaacga gagcgaatgg agttcagaga ttgtaataag acctgcaagc
     5881 acttcttatt gtgcccttgg cataatgcac tcttgcagcg ataaattcca tgaaataaga
     5941 aaaatatttg aatccacatg cgaaagcata cagcactggg ggaatgaaac tgaagttctt
     6001 tcaaagaaag aaaatatatt aaattgccta aacactttcc aagaaaattt tcttgattac
     6061 cagaaacctt ttttaatgca accattatgg aaaacaaaag gcaagagccc aatattagat
     6121 caaaatgcct ttgacatatt catctggagt gattttgctt tgtgtcgaac ctttattgat
     6181 agatcctcat ataacaattc atctgccagc agactaatga gatcatcagc cagactcgcc
     6241 agaatggtgt atgaaattgc gagcaaaaac aagacaaaca tcaatagaat ttatactgag
     6301 atgaactttg gatatcaaag tgataaagag ttctcgctaa acggaaaaat aacaaaagct
     6361 tatatggatc acccaagaag aacaaaaccc atcctcccac catcagtatt aaaagaaata
     6421 attttaaatg gcgggcaaaa taaattaagc ccagagagac gctttgatca aagcgtttat
     6481 tatactgcat ctcacctgtt tgatgaaata aagtaatttt tcttggtggc tcgccagccc
     6541 tgaattcgcc catcaaaaat cagtagcgga acaggcatac tagacatgct cgcttttacg
     6601 gcgagcatct tgacggagtt tgagacgcaa tggaacagca accaactcta tgcatcacaa
     6661 gctcaaaaat gattaccatg cattgaactc tagttacctt tgccaatgat tgctc
//
