LOCUS       CP063644                6173 bp    DNA     circular BCT 04-APR-2022
DEFINITION  Enterococcus faecium strain ICU-2-1 plasmid pZA2-1_05, complete
            sequence.
ACCESSION   CP063644
VERSION     CP063644.1
DBLINK      BioProject: PRJNA669626
            BioSample: SAMN16483150
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Both,A., Kruse,F., Mirwald,N., Franke,G., Christner,M., Huang,J.,
            Hansen,J.L., Kroger,N., Berneking,L., Lellek,H., Aepfelbacher,M. and
            Rohde,H.
  TITLE     Population dynamics in colonizing vancomycin-resistant Enterococcus
            faecium isolated from immunosuppressed patients
  JOURNAL   J Glob Antimicrob Resist 28, 267-273 (2022)
   PUBMED   35134550
REFERENCE   2  (bases 1 to 6173)
  AUTHORS   Both,A., Kruse,F., Christner,M., Huang,J., Mirwald,N., Franke,G. and
            Rohde,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) Institute of Medical Microbiology, Virology
            and Hygiene, University Medical Center Hamburg-Eppendorf,
            Martinistr. 52, Hamburg, Hamburg 20246, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 336.3x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/27/2020 18:41:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.13
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,927
            Genes (coding)                    :: 2,804
            CDSs (with protein)               :: 2,804
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 44 of 123
            Pseudo Genes (incomplete)         :: 70 of 123
            Pseudo Genes (internal stop)      :: 23 of 123
            Pseudo Genes (multiple problems)  :: 13 of 123
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="ICU-2-1"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pZA2-1_05"
                     /geo_loc_name="Germany: Hamburg"
                     /lat_lon="53.33 N 10.1 E"
                     /collection_date="2019-03-01"
                     /collected_by="Institute of Medical Microbiology, Virology
                     and Hygiene, University Medical Center Hamburg-Eppendorf"
     gene            1..945
                     /locus_tag="IQN02_15005"
     CDS             1..945
                     /locus_tag="IQN02_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="UOC57240.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKINGD
                     VENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFAYFS
                     KEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQIKQ
                     LEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
                     /name="RepB family plasmid replication initiator protein"
     gene            966..1499
                     /locus_tag="IQN02_15010"
     CDS             966..1499
                     /locus_tag="IQN02_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="UOC57241.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLREQL
                     ALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATETMD
                     EQTKNWKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            1481..1684
                     /locus_tag="IQN02_15015"
     CDS             1481..1684
                     /locus_tag="IQN02_15015"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOC57246.1"
                     /translation="MAAMEMKKEILMTKKHLLFGSILLGILSFSPMLLSVANKGNKKGE
                     LKLNPNYYNSQNEEVFVEKLDK"
                     /name="hypothetical protein"
     gene            2139..2363
                     /locus_tag="IQN02_15020"
     CDS             2139..2363
                     /locus_tag="IQN02_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="UOC57242.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEKC
                     WVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
                     /name="bacteriocin hiracin-JM79"
     gene            2383..2670
                     /locus_tag="IQN02_15025"
     CDS             2383..2670
                     /locus_tag="IQN02_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="UOC57243.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKSV
                     GEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
                     /name="bacteriocin immunity protein"
     gene            3120..3500
                     /locus_tag="IQN02_15030"
     CDS             3120..3500
                     /locus_tag="IQN02_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UOC57237.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3482..4396
                     /locus_tag="IQN02_15035"
     CDS             3482..4396
                     /locus_tag="IQN02_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="UOC57238.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKKKVRS
                     NKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTTEQPEI
                     RPTRRNHDQHVERGADYQQELSF"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4356..4634
                     /locus_tag="IQN02_15040"
     CDS             4356..4634
                     /locus_tag="IQN02_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOC57244.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDSQ
                     VKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
                     /name="hypothetical protein"
     gene            4641..5330
                     /locus_tag="IQN02_15045"
     CDS             4641..5330
                     /locus_tag="IQN02_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOC57239.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLPG
                     VIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQTEEA
                     CYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(5451..5537)
                     /locus_tag="IQN02_15050"
     CDS             complement(5451..5537)
                     /locus_tag="IQN02_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017548328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="UOC57245.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     BGC             1..6173
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2..945
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3483..4393
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.421"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggctaatg agattgtaaa ataccaccat gaattaaata caattccttt aagaaaattt
       61 acaccggttg aaatgaattt gtttttttcc atcgtgtcta gaatgcgtga aaaaggggat
      121 aagacagttc gttttacgtt tgaccaacta aaagatttaa gtaactacaa agcaactgca
      181 aatgtacgat ttgtagaaga tttaaaagca acttatgaaa aaatcttatc tttacgcttt
      241 ggtcgtagaa gtgagtctgg gctttcttac gatatgtttg taatgtttac agaatttaaa
      301 atcaacgggg acgtagaaaa tccttacgta gatatacgaa tttacgaaaa agcactgcct
      361 ttgttaaata atttagacga gtgggtacgc tattctttac aacaattcaa cgagctacaa
      421 agtggctatg caaagactat gtttcgtctg ttaaagcaat acagaaccaa aggtttcgca
      481 tatttctcca aagaagactt tcttgaatta ttagatattc ctaaaagtta taagcaacct
      541 gatatagata aacgagttat aaaacctata cgtcaagaac taactgcaat cttcaaagga
      601 ctaacaatca aaaagaaata cggaaaagga cggggaaaac ctgttattgg ctatcaattt
      661 acgttcaaac ctgagatcaa aaaagcagac gactttaaca aacatgcacc aaccaaagaa
      721 gaccaaaata aagcattggc tttagctgat aaacaaaaag aatacgaaga acccgctaca
      781 acaattaacg aaaaaatcaa acagaagaaa cgagaacttg aactcctcca acaaatcaaa
      841 caactagaaa aagagaaaaa agctcttgaa aaacaaacag agtccgcact ttcggaaaaa
      901 gagaaaaaaa gtttattatc tgatttagat aatttattcc agtagcaacc taggaggcaa
      961 ccagtatgca accacaagaa acctttacaa taaaggaatt gtcagatctt tttaagatga
     1021 gtaggcaagc aatatcgaaa catatccaaa aattagattc ttccatgata gctaaaaatg
     1081 agcgtggtta taaggttgta ttgcgttctg gagtattgca acttgctcgc aacttagaca
     1141 atcaaaggtt gctagataag ttgcataaac atgaacaaaa caacgataaa aatgcttata
     1201 taccaaacaa aaacgtggtt gcaaacaagt tgctagatca gttgcgtgaa gaaaatgaat
     1261 ttttacgaga acaacttgcc ctcaaagagc aaaataattc tcatatacaa aaactactcg
     1321 accagcaaca acaactcacc ctgcaagcca accaacaaat cgaaaaactt caggagcaac
     1381 tgcagttgac ttatacggaa gagtcttcag agaatagttc aacgactctt tctgaagcaa
     1441 cagaaacaat ggacgaacaa acaaagaatt ggaaaaaatg gtggcagcta tggaaatgaa
     1501 gaaggaaata cttatgacaa aaaaacactt gctattcggt tctatcctac taggaatatt
     1561 aagctttagc ccaatgttat tatcagtggc caacaaagga aataaaaaag gggaactaaa
     1621 actcaatcct aattactaca acagtcaaaa tgaggaagta tttgtagaaa aacttgataa
     1681 ataagaaaag actgctcgta cgagcagtct ttctgaaaga gattatccta ttattaacct
     1741 tagaaaagaa taataaatga atatgagata tgtaaaaagc ataacaaaca caaacaaaaa
     1801 taacaagtat ttaaaaaatg accgatcaat attcacaaag aattagaaag aaacaaccgc
     1861 agaaaaccaa gccttaaaaa gagcttttaa cctagaccaa taaaaattca caaaatcagc
     1921 gcctggatcc cttggtacag gcgttacttg gtagtataca cattatgtct actagctgtt
     1981 taaataactg atcttataat tttatttaaa cgattatgaa ttcaaaacta ctttttatga
     2041 cgttaatcat tgttatataa taaaatgtta tataataaaa ttactaagat tatttaatat
     2101 aacaagcaat tttaaaaaaa cgaaaaggaa aaacaatcat gaaaaagaaa gtattaaaac
     2161 attgtgttat tctaggaata ttaggaactt gtctagctgg catcggtaca ggaataaaag
     2221 ttgatgcagc tacttactat ggaaatggtc tttattgtaa caaagaaaaa tgttgggtag
     2281 attggaatca agctaaagga gaaattggaa aaattattgt taatggttgg gttaatcatg
     2341 gtccatgggc acctagaagg tagtggtagg gaggttatta taatggattt tactaaagaa
     2401 gaaaaacttt taaatgcaat tagtaaagta tacaatgaag caactataga tgactatcct
     2461 gacttaaaag aaaagctctt tctttattct aaagaaatca gtgagggaaa aagtgttggt
     2521 gaagttagta tgaaattaag tagttttctt ggaagatata ttttaaaaca taaatttgga
     2581 ttacctaaat ctttaataga attacaagaa attgttagta aggaatctca agtatataga
     2641 ggatgggctt ctattggtat ttggagttaa ttctttggaa taaaatgaaa atgggactga
     2701 agaatcaatt ctgattcatc agtcccataa attatagagc cgaaaaatta tcatatttat
     2761 agatttatta atagtaaatg gcataagaat ctttgaacaa cgaaaaaaat gtagctctac
     2821 ctaattattg atttgcaaca atttcaccga aggtgaaaca agcgaaactt tacaaaaagt
     2881 ggctagcatt tgctagtctt ttttgtattg tggagagtgg gggggagggg gattcaaggg
     2941 ggagcttgcg cccctttgac aagcggttat ttgcaccaaa tacgtaggta tttggctagc
     3001 aaatatagag cttgccaagc cctttagtga gctttagtga gctttactta ttgtaccttt
     3061 ttaggtacaa tggtattgcg aacgatacgt ccgtaggacg tactgaaagg gtgggactta
     3121 tgagcgaaca aaatcaatat ttggctagca atcattctaa aaaatatacc tatcgatccg
     3181 agccaaaaca aatcagtttt cgagtgagcg aatccgaatt tgcaaagctg aagcagtcag
     3241 ctgaagcttt gcaaatgagt gtgccggctt tcgtgaaagc caaggcacaa aacgcgcgtc
     3301 tggtgacacc gaaagtggcg ccagacattg ctcaggcaat cgcgcgcgat ttagccaaag
     3361 ctggcgggaa tatcaatcaa attgccaagt ggtgcaacac acaccaacac gacgttgatc
     3421 ctggcgacgc ccaacgctta tccgaaaacc taaaaatcat gcaaaaggag ctacaaaaga
     3481 tatggcaaca gttaaagtaa gccgaaccac ttcttgtagc cgagcgatca attacgccga
     3541 accacgagca accgtcaaaa cagggataaa ctgtgacatt gattatgcca aaagcgaaat
     3601 gaaacaaatt cgcatgctgt atggaaaaga cgatcgtgta caagcacact tattgatcca
     3661 gtcttttcga ccaggagaaa ttaccgctga aaaagccaat caactagggg cagagtatgc
     3721 gaaaaaaata gcccctgacc accaaatcgc gatttacaca catacagaca aagaccatat
     3781 tcataaccac attgtcatta actccgtcaa tctggaaact gggaacaaat ttcaagccca
     3841 tggtcaagca tttttaaaca aatgttatga cctaaatgac gagatttgtc ttgaacatga
     3901 cttaacaatc actgaacgag ggaagaaacc cgaaaaacga accatgtctg aaatcaaatt
     3961 aaaagaaaaa aatgaacctg tttggaaaga cgaaattcgg tttgcgattg atcaaacaat
     4021 gaaaaacaaa aaaactcgaa cgtataacca attttgtgat tctcttaaaa cattcggcat
     4081 tcattgcttg aatcgtggga aaaattttac ttacgaattg atccacaaaa agaaaaaagt
     4141 acgatccaac aaactaggaa aggattacga aaaggagacg attttacatg agctggacag
     4201 acgagaacga gcctacaatc aacaacgaac caacgagcta cgaagagctc gagctagcat
     4261 tcgcgaaact gcaaaagaag tacgaagaga acaacgaact cttacgactg aacaaccgga
     4321 aattagaccg actcgaagaa accatgacca acacgttgaa agaggagcag actaccaaca
     4381 agaattatcg ttctaaaacg ttacaagaca tagaaaatga aataaagaaa caacgtgctt
     4441 ttcttgcacc ttataccaaa gaaagaatac aaactacaat tgattcacaa gtcaaaaaat
     4501 tccaccatgc gctattaaac cagcgaaata aagaacagaa aaaagtaatt atgcagcgat
     4561 caatggatat tcttgccccc ttatgttgct taatcatgac actgttagcc ttttggctgg
     4621 tatggggaga ataaatagaa atggcattct atttacccta tctactcatt tttgccagta
     4681 ttttaggaat tatttggctt agctataagg cttatcaaac aagatattca ttaaaaggct
     4741 ctaaaattcg atttaagagg tttttattgc caggggttat atttacatta tcaatcgttt
     4801 taagtggttt attcggcgtt ttagagggaa ataaacgtat ttcaagaagt attttattag
     4861 gggatatcac tcaaaagtat gaagcagcat gtgataaaaa gaaaaaggag caagtgttaa
     4921 cacaaaaaat ggaagagttt accacttgtt atgaggaaat gaacgatatt tttgtgaatc
     4981 aagaaaaacg tttgacggat aaaaacatgg aaaaactcac tcgtctttat caaaacttgc
     5041 cagaaaaaca gcaaaaagag gttcaagaca actatgagcg aaccaaaaaa gacgtgcaat
     5101 attttaaaga tacgcagaca gaagaagctt gttataattt attcagtgat acaatccctt
     5161 tttcaactag tgaacaggaa cgaaaagaac ggcaacaatc agttacctac gaacgttata
     5221 aagcactgct tcagcaagca acgaacatac aaaatccaac gaaaaaagaa acggcattaa
     5281 attatctaaa aagtgtcaag gaatggttag atcagcaaca gcaaaattga atttgatcgg
     5341 caatcatgtt aaactaaaca tgcaaatgat caaacattag atacacgaaa gccccagaga
     5401 ggtcggattc tctggggctt tttttcaggt tgatgtcgcc tttattgatt ttacttcttg
     5461 tttcggttat tcaaccaata actgaataaa gagacacaaa cttctataag aagggggcaa
     5521 atgaacaatt caaacattag atacacctcc ttccagtcgc aagactgtcg gataggcgac
     5581 aagttattat atcttaaaaa attaaacttg tgtggtgttt ttaagtatcg cttattttag
     5641 ggttgttagg gcttgccctg accgcctgta aggcgcttta aagctagata aggattttta
     5701 tccaagactg tcttaaaacg gcttagataa gcattcagaa ccgctcaaag gcctctttac
     5761 caacatgttt ttagcaaagg agcctttagg cgactgcgca gtacaatgtg agagcgtagc
     5821 gatcacacct ttttggcgac cgtaggaagc aaggttttgg attggcagtt ttgaaaaatg
     5881 cgcgaacctg tttaaagaaa aaagaagtgg tttttaggcg ggaaaattag cccccctctt
     5941 tttatatctc tttttaaacc tctttttaaa cctctttacc gtaagctgtg tccttactct
     6001 accaatggat acagaatgtt atgaaggaca ttttgtctgt ttatgaagga cattttgtct
     6061 gtttatgaag gacattttgt ctgtttatga aggacatttt gtctgtttga acgtcaaata
     6121 tgaatgtctt gacatttaaa aaatgtcatt cataattgga ttgaggtgat tat
//
