LOCUS       CP063643                7163 bp    DNA     circular BCT 04-APR-2022
DEFINITION  Enterococcus faecium strain ICU-2-1 plasmid pZA2-1_04, complete
            sequence.
ACCESSION   CP063643
VERSION     CP063643.1
DBLINK      BioProject: PRJNA669626
            BioSample: SAMN16483150
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7163)
  AUTHORS   Both,A., Kruse,F., Mirwald,N., Franke,G., Christner,M., Huang,J.,
            Hansen,J.L., Kroger,N., Berneking,L., Lellek,H., Aepfelbacher,M. and
            Rohde,H.
  TITLE     Population dynamics in colonizing vancomycin-resistant Enterococcus
            faecium isolated from immunosuppressed patients
  JOURNAL   J Glob Antimicrob Resist 28, 267-273 (2022)
   PUBMED   35134550
REFERENCE   2  (bases 1 to 7163)
  AUTHORS   Both,A., Kruse,F., Christner,M., Huang,J., Mirwald,N., Franke,G. and
            Rohde,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) Institute of Medical Microbiology, Virology
            and Hygiene, University Medical Center Hamburg-Eppendorf,
            Martinistr. 52, Hamburg, Hamburg 20246, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 336.3x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/27/2020 18:41:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.13
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,927
            Genes (coding)                    :: 2,804
            CDSs (with protein)               :: 2,804
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 44 of 123
            Pseudo Genes (incomplete)         :: 70 of 123
            Pseudo Genes (internal stop)      :: 23 of 123
            Pseudo Genes (multiple problems)  :: 13 of 123
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="ICU-2-1"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pZA2-1_04"
                     /geo_loc_name="Germany: Hamburg"
                     /lat_lon="53.33 N 10.1 E"
                     /collection_date="2019-03-01"
                     /collected_by="Institute of Medical Microbiology, Virology
                     and Hygiene, University Medical Center Hamburg-Eppendorf"
     gene            138..224
                     /locus_tag="IQN02_14960"
     CDS             138..224
                     /locus_tag="IQN02_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017548328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="UOC57236.1"
                     /translation="MFEQFICPLLVGVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(345..1034)
                     /locus_tag="IQN02_14965"
     CDS             complement(345..1034)
                     /locus_tag="IQN02_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOC57231.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTTCY
                     KEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYENLFQQATNMQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(1274..2188)
                     /locus_tag="IQN02_14970"
     CDS             complement(1274..2188)
                     /locus_tag="IQN02_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="UOC57232.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQTAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2170..2550)
                     /locus_tag="IQN02_14975"
     CDS             complement(2170..2550)
                     /locus_tag="IQN02_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UOC57233.1"
                     /translation="MSEQNQNLASDHSKKYTYRSKPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(2852..3274)
                     /locus_tag="IQN02_14980"
     CDS             complement(2852..3274)
                     /locus_tag="IQN02_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOC57235.1"
                     /translation="MVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDHS
                     ERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNKKQ
                     LDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
                     /name="hypothetical protein"
     gene            complement(3268..4422)
                     /locus_tag="IQN02_14985"
     CDS             complement(3268..4422)
                     /locus_tag="IQN02_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="UOC57234.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQPI
                     EEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDA
                     DNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIK
                     LLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDNFIMI
                     NWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKSLNQDQ
                     IIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQ
                     GGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
                     /name="peptidase C47"
     gene            complement(4981..5285)
                     /locus_tag="IQN02_14990"
                     /pseudo=""
     CDS             complement(4981..5285)
                     /locus_tag="IQN02_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(5369..5890)
                     /locus_tag="IQN02_14995"
     CDS             complement(5369..5890)
                     /locus_tag="IQN02_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="UOC57229.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKVV
                     LRSGVLQLASNLGNQRLLDRLHEYEQENDKNVYISRKNVVVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEEPEEEVLTSVNKENNNAI
                     TTEKKWWKFW"
                     /name="DUF536 domain-containing protein"
     gene            complement(5919..6656)
                     /locus_tag="IQN02_15000"
     CDS             complement(5919..6656)
                     /locus_tag="IQN02_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UOC57230.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEIF
                     TFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDKVN
                     ETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKGHWF
                     VKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAYKGNK
                     IGSLQIYFEENIPSITLDNWLEK"
                     /name="replication initiation protein"
     Typing          complement(5920..6656)
                     /name="rep_cluster_1742"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1278..2188)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.671"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gccttacagg cggtcagggc aagccctaac aacccgaaaa taaggtatac ttaaaatcgc
       61 cacacagatt taattttgta gggtataata acttgtcgcc tatccgacag tcttgcgact
      121 ggaaggaggt gtatctaatg tttgaacagt tcatttgccc ccttcttgta ggagtttgtg
      181 tctctttatt cagttattgg ttgaataacc gaaacaagaa gtaaaatcag taaaggcgac
      241 atcaacctga aaaaaagccc cagagaatcc gacctctctg gggctttcgt gtatctaatg
      301 tttgatcatt tgcacattca gtttaacatg agtttcaatc aaattcaatt ttgctgttgc
      361 tgatctaacc attccttgac actttttaga tagtttaagg cggtttcttt ttttgtcgga
      421 ttttgcatgt tagttgcttg ttgaaacagg ttttcgtagc gttcatatgt gacagattgt
      481 tgtttttctt ttttttcttc ttcactggca aaccaagggt ttgtgtctcc aaatagatca
      541 ctacaggctt cttctatctt tgtatctttt acatattgca cgtctttttt ggttcgctca
      601 tagttgtctt gaacctcttt ttgctgtttt tctggcaagt tttgataaag acgagtgagt
      661 ttttccatgt ttttatccgt caaacgtttt tcttgattca caaaaatatc gttcatttcc
      721 ttataacaag tggtaaactc ttccattttt tgtgctaatg cttgctcttt tttcttttta
      781 tcacgcgctg attcatactt ttgagtgaca tttcctaata aaatacttct agaaacgcgc
      841 ttatttccct ctaaaacgcc gaataagccg cttgaaacga ttatcaagga aaatatacag
      901 ccaagaaaga aaaatcgctt aaatcgaatt ttagagcctt ttaatgaata tcttgtttga
      961 aaaattttat agatgagcca aatacttcct gaaatactaa caaaaatgag tagatagggt
     1021 aaataaaatg ccattttagc ctccccatac taaccaaaat gcgaacagtg tcaggactaa
     1081 gcaacataag ggggcaagaa aatccaatga tcgttgaata agcacctttt tctgttcctt
     1141 atttcgctgg tctaatagct cttgatggag ttttttaact tgtaaatcaa tcgtagtttg
     1201 tatcctttct tgcgtataag gaacgagaaa agcacgttgt ttctttattt cgttttctat
     1261 gtcttttaac gttttagagc gataattctt gttggtggtc tgttcctctt tcaacgtgtt
     1321 gttcatggtt tcttcgagcc ggtctaattt ccggttgttc aggcgtaaga gttcgttgtt
     1381 ctcttcgtac ttcttctgca gtttcgcgaa tgctagctcg agctcttcgt agctcgttgg
     1441 ttcgctgttg gttataggtt cgttctcgtc tgtccagctc atgtaaaatc gtctcctttt
     1501 cgtagtcctt tcctagtctg ttggatcgta cttttttctt tttgtggatc aattcgtaag
     1561 tgaaattttt cccgcgattg aagcaatgaa tccccaatgt tttgagggaa ttacaaaatt
     1621 ggtcataggt tctggttttt ttgtttttca ttgtttgatc gatcgcaatc cgaatttcgt
     1681 ctttccaaac aggttcattt ttttctttta attttatttc agacatggtt cgtttttctg
     1741 gtttctttcc tcgctcagtg attggtaagt tatgtgcaag acaaatctcg tcatttaggt
     1801 cataacattt gtttagaaat gcttgaccat gagcttgaaa tttttttcca gtctccagat
     1861 taacggagtt aatgacaatg tgattatgaa tatggtcttt gtctgtatgt gtgtaaatag
     1921 ctgtttggtg atcgggggct attttttttg catactctgc ccctagttga ttagcttttt
     1981 cagcggtaat ttctcctggt cgaaaagatt gaatcaataa gtgtgcctgt acacggtcgt
     2041 cttttccata cagcatgcga atttgtttca tttcactttt ggcataatcg acgtcacagt
     2101 ttattcctgt tttaatggtt gctcgtggtt cagcgtaatt aattgcacga ctacaagaag
     2161 tggttcggct tactttaact gttgccatat cttttgtagc tccttttgca tgatttttag
     2221 gttttcggat aagcgttggg cgtctccagg agcaacgtca tgttggtgtg tattgcacca
     2281 cttggcaatt tgattgatat tccctccagc tttggctaaa tcgcgcgcga ttgcctgggc
     2341 aatgtctggc gccactttcg gtgtcaccag acgcgcgttt tgtgccttgg ctttcacgaa
     2401 agccggcaca ctcatttgca aagcttcagc tgactgcttc agctttgcaa attcagattc
     2461 gctcactcga aaactgattt gttttggctt ggatcgatag gtatattttt tagaatgatc
     2521 gctagccaaa ttttgatttt gttcgctcat aagtcccacc ctttcagtac gtcctacgga
     2581 cgtatcgttc gcaataccat tgtacctaaa aaggcacaat gagtaaagct caccaaaagg
     2641 gcttggcaag ctctatattt gctagccaaa tacctacgta tttggtgcaa ataaccgctt
     2701 ttcaaagggg cgcaagctcc cccttgaatc cccctcaaaa cccacgctcc acaatacaaa
     2761 aaaggctagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     2821 ttattgtttt gtaatatcaa acaactattc atcaatcagc tttaaaaaat tcaattggtt
     2881 tagctgtccc ttctgtccca tcattcgttg tcaaaataaa tacgagcttt tttggagaat
     2941 ccaaactaaa agtgtaatct aattgttttt tattcgagcg cagtgttagt aaattatttg
     3001 ttgctttgta aatgaaaact tgttcattta tatttaaatt tttatcaatg atttgaattt
     3061 tatgattgtt ctcttttact aagatggtat attgttctat attttttgaa gtatttaaat
     3121 tagtgattac tagacgttct gaatgatctt tagtttgcca agttcctaga aaaggaatca
     3181 gatcagtctt ttgtatttct tgtttttttg aacaactagt taagaaaaga actaacacta
     3241 gacccataac taaagcaatg agtttattta ccaatttgta atggtattgt cccaagtaaa
     3301 accacctcca ggtacaggaa ttgtaactgg tcctgcattt gcacttatta cagttgggta
     3361 gttataccaa ggattccaca catagtaagt ttgataacca ccttgattga cccacccaaa
     3421 taatgcaagt gcatgacggc tgttactata atttccataa ccagcagttc ccatataaat
     3481 ggcatttcct tttcttagtt gtgtttggac ctctgaaagg tttagtattc gtgctatttt
     3541 tttaggataa acaccacagt aatttgcata ttttattatt tgatcttgat taagactttt
     3601 tgattcaatt tgactgttgt ttaaattagg ataggtataa tgcataatgg ctctagctga
     3661 agtagcacgt acatctgttt tgttacataa gatagcagct tctacgtaag cagcgcacca
     3721 cggaaaactt gtttgggttt ctgctatacg ccaattaatc ataataaagt tatctccgga
     3781 ggagacactt tttacacgtt taaccgcagg aaaattttgt tttttagaaa aatttttaat
     3841 tttatttttt atatttacgg atataagatc atttttttct gtaagggaag aaacaccatt
     3901 tttttctgta cttaaaggtg atttccaaag tagtttaatt tgtccattat cggaataata
     3961 aatgctatta tcttgaaaaa ataaggttgt atctgttata ttactatcta ctagattttg
     4021 aagttctttt gctaaaaatt gtgaaatact tcctgaatat tctccatttg tatttttttt
     4081 gacggtataa atatatttaa tcgtattatc agcgtctgaa aatactggaa agaaaataga
     4141 actatttgcc gtattttctc catcgagcat tttaaatggc gaaccaagag tatagttaat
     4201 atcagtttcg gtgttatttt ccattaaaag aattgaagtt atggagtgtt caaaatcagt
     4261 tttcgcaaaa ttaattgctt gttcttcaat tggttgggag ttgatattga aattagaatt
     4321 ttctgaagca taggtatttg taattcctat actaaaaatt ccaatcatca aggaaaagag
     4381 gagacctaga taaattttct ttttgggttt caaaataatc acgcctttgt gttaaattta
     4441 agtggtatac acctataatt aaaatataca atatgcgata acaaattttt gttatagttc
     4501 ataaaatatt gttttgggaa cataatcgtt atttatagcg ttagttaaca taatgtcgac
     4561 tatgccaagt agcccctcta tcaagggcta tagctgctca ctttgtgatt tttcattggt
     4621 ctaggtcaaa agcttttttt aaggcttgat tttctgcagt tgcttctttc cgtttctttg
     4681 tgaattttta ccagtcattt tttactggat tgttattttt tataaaatat gataaaattt
     4741 tggtagcgct ttataagcgc gttgcttttt caagaataaa ctctcattat ttttttaact
     4801 ggctgctcga aagagcagtc tttttttata ctttatacaa agtataaaaa actcagcaaa
     4861 ataattggaa aaacactatg aagaaataaa tttttatgtt tattaaaaat aataatcaga
     4921 gtattgatta ctaatagcaa acttagaaaa aatattaata accaagtggc aattgaatgg
     4981 ctaaaataaa agaatttgga ctgaattaag gtaaaaacta aacttaaaaa aattacaaaa
     5041 tgaaataata gtattagtgt ttttgtatga ttttttaaca taactttttc ttctcctaac
     5101 aaaatattgt ctagtgataa atcaaataac ttagcaaatt gaattaaatt ttctgtatct
     5161 ggtattgttc taccattttc ccaattagaa atggtttttt tagatactaa aagcatttca
     5221 gctaaatctt cttgagttag ttgtttttct attctttttt gtttgattac tttacttata
     5281 gtcatttgat cacctaaaca tattttttat ttttagaaag ataaagtcta tagaaattga
     5341 tttcttcttt atggaaagtt ggtaattgtt accaaaattt ccaccacttt ttttctgttg
     5401 ttattgcatt gttgttttct ttattgacag aagttaatac ctcttcttca ggttcttccg
     5461 tataagctaa ttgtaactgt tcttgcagtt tttcgatctg ttgatttgcc tgcaaggtga
     5521 gttgttgttg ctggtcgagt aatttttgca tattagaatt attttgctct ttgagagaaa
     5581 gttgttctcg taaaaattca ttttcttcac gcaacttatc tagcaactcg tttacaacca
     5641 cgttttttct tgatatataa acgtttttat cgttttcttg ttcatactca tgcaacctat
     5701 ctagtaacct ttggttacct aagttgctag caagttgcaa tactccagaa cgtaatacaa
     5761 ccttataacc acgctcattt ttagctaata aggacgggtc taatttatca acatgtttag
     5821 aaattgcttg cctacttatt tcaaataatt cagataattc ctttattgta aaggtttctt
     5881 tgggttgcat gataattgcc tcctaaattg ttaggttatt atttctctaa ccaattatct
     5941 aaggtaattg aaggtatatt ttcttcaaaa taaatttgca gagatcctat cttatttcct
     6001 ttgtaggctt taattttttt gatttcaaaa gttttaaaaa tagcagggtg agttgaatta
     6061 acgtttaaaa attctttttt cgcttgggca aaaatttgtt tatcaatatc tctcatgcga
     6121 taactttctg gtattgctaa tagagctcta aaatcttcta tttttacaaa ccagtgacct
     6181 tttttagatc ttctgtcttt atgagccata agttggcgat ataactcttt aacgtagcta
     6241 ctctttaagt ttgtcatatt ttctaattcg aaacgagtaa aattagttcc gattgaatta
     6301 agtatatatt caaattcttc agaaaccttt attaataatg tttcattcac tttatcgatt
     6361 ttaaattttt gaaacagatt taattgttca tatgtttttt cgtcttcata aataaaagac
     6421 aaagtaatga ttttttttga catttttatt aaatcattag caaaacggtc gccacgtgca
     6481 tttatattgt aatcaattaa ttcttttatt tgtttaaatg taaaaatttc ttcttttgta
     6541 ccttttcttt tcattttaga acatattccc caaaataaat ccatatctat aggggtaaat
     6601 tttcttaaag ggacagaatt taatccatta ttatatcgaa ctatatcgtt actcaataaa
     6661 aatcacctca ataaattatg ttatcataga cataagaggg tgtaaaatac aatttatccc
     6721 ccatagtgtg acaatttatc ccccatagcg tgacaattta tcccccatag tgtgacaatt
     6781 tatcccccat agtgtgacaa tttatccccc actacctatg tcaatactta ctctcccata
     6841 aggttgctaa tccctaacag ttattattaa cagtatatct aacagtatat ctaacagtat
     6901 atcttaagag cacatttaat ttcccgccta aaaaactcct acttttacct ccaaaaaggt
     6961 tcgcgaattt ttctaaacta gaaaacttgc tccctacggt cgctaaaaag gtgtgatcgc
     7021 tacgctctca cattgtacct gcgcagtcgc ctccggctcc tttgctaaaa ccgttgttca
     7081 aagaggaggt gggagaatct gagagcttgt ctaagccgtt ctaagacaat tcttgattaa
     7141 ctcccttaat ctagtcgaga agc
//
