LOCUS       CP063638                6831 bp    DNA     circular BCT 04-APR-2022
DEFINITION  Enterococcus faecium strain ICU-5-1 plasmid pZA5-1_05, complete
            sequence.
ACCESSION   CP063638
VERSION     CP063638.1
DBLINK      BioProject: PRJNA669626
            BioSample: SAMN16483151
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6831)
  AUTHORS   Both,A., Kruse,F., Mirwald,N., Franke,G., Christner,M., Huang,J.,
            Hansen,J.L., Kroger,N., Berneking,L., Lellek,H., Aepfelbacher,M. and
            Rohde,H.
  TITLE     Population dynamics in colonizing vancomycin-resistant Enterococcus
            faecium isolated from immunosuppressed patients
  JOURNAL   J Glob Antimicrob Resist 28, 267-273 (2022)
   PUBMED   35134550
REFERENCE   2  (bases 1 to 6831)
  AUTHORS   Both,A., Kruse,F., Christner,M., Huang,J., Mirwald,N., Franke,G. and
            Rohde,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) Institute of Medical Microbiology, Virology
            and Hygiene, University Medical Center Hamburg-Eppendorf,
            Martinistr. 52, Hamburg, Hamburg 20246, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 124.1x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/27/2020 18:40:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.13
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 35 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 10 of 99
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="ICU-5-1"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pZA5-1_05"
                     /geo_loc_name="Germany: Hamburg"
                     /lat_lon="53.33 N 10.1 E"
                     /collection_date="2019-03-01"
                     /collected_by="Institute of Medical Microbiology, Virology
                     and Hygiene, University Medical Center Hamburg-Eppendorf"
     gene            join(6223..6831,1..546)
                     /locus_tag="IQN03_15435"
     CDS             join(6223..6831,1..546)
                     /locus_tag="IQN03_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="UOE17652.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQPI
                     EEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDA
                     DNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIK
                     LLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDNFIMI
                     NWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKSLNQDQ
                     IIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQ
                     GGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
                     /name="peptidase C47"
     gene            519..962
                     /locus_tag="IQN03_15440"
     CDS             519..962
                     /locus_tag="IQN03_15440"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOE17653.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGT
                     WQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLT
                     LRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
                     /name="hypothetical protein"
     gene            1264..1644
                     /locus_tag="IQN03_15445"
     CDS             1264..1644
                     /locus_tag="IQN03_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UOE17654.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASGDA
                     QRLSENLKIMQKELQKIWQQLR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1626..2540
                     /locus_tag="IQN03_15450"
     CDS             1626..2540
                     /locus_tag="IQN03_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="UOE17655.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQTAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2780..3469
                     /locus_tag="IQN03_15455"
     CDS             2780..3469
                     /locus_tag="IQN03_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOE17656.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(3590..3676)
                     /locus_tag="IQN03_15460"
     CDS             complement(3590..3676)
                     /locus_tag="IQN03_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017548328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="UOE17657.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            4321..5058
                     /locus_tag="IQN03_15465"
     CDS             4321..5058
                     /locus_tag="IQN03_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UOE17649.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEIF
                     TFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDKVN
                     ETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKGHWF
                     VKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAYKGNK
                     IGSLQIYFEENIPSITLDNWLEK"
                     /name="replication initiation protein"
     gene            5086..5625
                     /locus_tag="IQN03_15470"
     CDS             5086..5625
                     /locus_tag="IQN03_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="UOE17650.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKVV
                     LRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEETESI
                     EKHTKKKKQKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            5641..5811
                     /locus_tag="IQN03_15475"
     CDS             5641..5811
                     /locus_tag="IQN03_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOE17651.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     Typing          4322..5058
                     /name="rep_cluster_1742"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1627..2537
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.342"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttcctgcgg ttaaacgtgt aaaaagtgtc tcctccggag ataactttat tatgattaat
       61 tggcgtatag cagaaaccca aacaagtttt ccgtggtgtg ctgcttacgt agaagctgct
      121 atcttatgta acaaaacaga tgtacgtgct acttcagcta gagccattat gcattatacc
      181 tatcctaatt taaacaacag tcaaattgaa tcaaaaagtc ttaatcaaga tcaaataata
      241 aaatatgcaa attactgtgg tgtttatcct aaaaaagtag cacgaatact aaacctttca
      301 gaggtccaaa cacaactaag aaaaggaaat gccatttata tgggaactgc tggttatgga
      361 aattatagta acagccgtca tgcacttgca ttatttgggt gggtcaatca aggtggttat
      421 caaacttact atgtgtggaa tccttggtat aactacccaa ctgtaataag tgcaaatgca
      481 ggaccagtta caattcctgt acctggaggt ggttttactt gggacaatac cattacaaat
      541 tggtaaataa acccattgct ttagttatgg gtctagtgtt agttcttttc ttaactagtt
      601 gttcaaaaaa acaagaaata caaaagactg atctgattcc ttttctagga acttggcaaa
      661 ctaaagatca ttcagaacgt ctagtaatca ctaatttaaa tacttcaaaa aatatagaac
      721 aatataccat cttagtaaaa gagaacaatc ataaaattca aatcattgat aaaaatttaa
      781 atataaatga acaagttttc atttacaaag caacaaataa tttactaaca ctgcgctcta
      841 ataaaaaaca attagattac acttttagtt tggattctcc aaaaaagctc gtatttattt
      901 tgacaacgaa tgatgggaca gaagggacag ctaaaccaat tgaatttttt aaagctgatt
      961 gatgaatagt tgtttgatat tacaaaacaa taatttcacc gaaggtgaaa caagcgaaac
     1021 tttacaaaaa gtggctagca tttgctagcc ttttttgtat tgtggagcgt ggtttttgag
     1081 ggggattcaa gggggagctt gcgccccttt gacaagcggt tatttgcacc aaatacgtag
     1141 gtatttggct agcaaatata gagcttgcca aaccctttta gtgagcttta ctcattgtac
     1201 ctttttaggt acaatggtat tgcgaacgat acgtccgtag gacgtactga aagggtggga
     1261 cttatgagcg aacaaaatca aaatttggct agcaattctt ctaaaaaata tacctatcga
     1321 tccgagccaa aacaaatcag ttttcgagtg agcgaatccg aatttgcaaa gctgaagcaa
     1381 tcggctgaag ctttgcaaat gagtgtgccg gctttcgtga aagccaaggc acaaaacgcg
     1441 cgtctggtga caccgaaagt ggcgccagac attgcccagg caatcgcgcg cgatttagcc
     1501 aaagctggag ggaatatcaa tcaaattgct aagtggtgca atacacacca acatgacgtt
     1561 gcttctggtg acgctcaacg tttatccgaa aacctaaaaa tcatgcaaaa ggagctacaa
     1621 aagatatggc aacaattaag gtaagccgaa ccacttcttg tagtcgtgca attaattacg
     1681 cagaaccacg agcaaccatt aaaacaggaa taaactgtga cgtcgattat gccaaaagtg
     1741 aaatgaaaca aattcgcatg ctgtatggaa aagacgaccg tgtacaggca cacttattga
     1801 ttcaatcttt tcgaccagga gaaattaccg ctgaaaaagc taatcaacta ggggcagagt
     1861 atgcaaaaaa aatagccccc gatcaccaaa cagctattta cacacataca gacaaagacc
     1921 atattcataa tcacattgtc attaactccg ttaatctgga gactggaaaa aaatttcaag
     1981 ctcatggtca agcatttcta aacaaatgtt atgacctaaa tgacgagatt tgtcttgcac
     2041 ataacttacc aatcactgag cgaggaaaga aaccagaaaa acgaaccatg tctgaaataa
     2101 aattaaaaga aaaaaatgaa cctgtttgga aagacgaaat tcggattgcg atcgatcaaa
     2161 caatgaaaaa caaaaaaacc agaacctatg accaattttg taattccctc aaaacattgg
     2221 ggattcattg cttcaatcgc gggaaaaatt tcacttacga attgatccac aaaaagaaaa
     2281 aagtacgatc caacagacta ggaaaggact acgaaaagga gacgatttta catgagctgg
     2341 acagacgaga acgaacctat aaccaacagc gaaccaacga gctacgaaga gctcgagcta
     2401 gcattcgcga aactgcagaa gaagtacgaa gagaacaacg aactcttacg cctgaacaac
     2461 cggaaattag accggctcga agaaaccatg aacaacacgt tgaaagagga acagaccacc
     2521 aacaagaatt atcgctctaa aacgttaaaa gacatagaaa acgaaataaa gaaacaacgt
     2581 gcttttctcg ttccttatac gcaagaaagg atacaaacta cgattgattt acaagttaaa
     2641 aaactccatc aagagctatt agaccagcga aataaggaac agaaaaaggt gcttattcaa
     2701 cgatcattgg attttcttgc ccccttatgt tgcttagtcc tgacactgtt cgcattttgg
     2761 ttagtatggg gaggctaaaa tggcatttta tttaccctat ctactcattt ttgttagtat
     2821 ttcaggaagt atttggctca tctataaaat ttttcaaaca agatattcat taaaaggctc
     2881 taaaattcga tttaagcgat ttttctttct tggctgtata ttttccttga taatcgtttc
     2941 aagcggctta ttcggcgttt tagagggaaa taagcgcgtt tctagaagta ttttattagg
     3001 aaatgtcact caaaagtatg aatcagcgcg tgataaaaag aaaaaagagc aagcattagc
     3061 acaaaaaatg gaagagttta ccacttgtta tgaggaaatg aacgatattt ttgtgaatca
     3121 agaaaaacgt ttgacggata aaaacatgga aaaactcact cgtctttatc aaaacttgcc
     3181 agaaaaacag caaaaagagg ttcaagacaa ctatgagcga accaaaaaag acgtgcaata
     3241 tgtaaaagat acaaagatag aagaagcctg tagtgatcta tttggagaca caaacccttg
     3301 gtttgccagt gaagaagaaa aaaaagaaaa acaacaatct gtcacatatg aacgctatga
     3361 aatcttactt caacaagcaa ctaacatgca aaatccgaca aaaaaagaaa ccgccttaaa
     3421 ctatctaaaa agtgtcaagg aatggttaga tcagcaacaa caaaattgaa tttgatttaa
     3481 aatcatgtta aactgaatgt gcaaatgatc aaacattaga tacacgaaag ccccagagag
     3541 gtcggattct ctggggcttt ttttcaggtt gatgtcgcct ttattgattt tacttcttgt
     3601 ttcggttatt caaccaataa ctgaataaag agacacaaac tcctacaaga agggggcaaa
     3661 tgaacagttc aaacattaga tacacctcct tccagtcgca agactgtcgg ataggcgaca
     3721 agttattata tcttaaaaaa ttaaatttgt gtggtgtttt taagtatcac ttgttttagg
     3781 gttgttaggg cttgccctga ccgcctgtaa ggcgcttctc gactagatta agggaaatta
     3841 atcaagaatt gtcttagaac ggcttagaca agctttcaga gtctcacacc tcctctttga
     3901 acaacggttt tagcaaagga gccggaggcg actgcgcagt ataatatgag aacatagcga
     3961 tcacaccttt ttagcgaccg tagggagcaa gttttccagt ttagaaaaat tcgcgaacct
     4021 ttttggagga aaaagtagga gttttttagg cgggaaattc aatgtgctct taagatatac
     4081 tgttagatat actgttagat atactgttaa taataactgt tagggattag caaccttatg
     4141 ggagagtaag tattgacata ggtagtgggg gataaattgt cacactatgg gggataaatt
     4201 gtcacactat gggggataaa ttgtcacact atgggggata aattgtcaca ctatggggga
     4261 taaattgtgt tttacatcct cttatgtcta tgataacata atttattgag gtgattttta
     4321 ttgagtaacg atatagttcg atataataat ggattaaatt ctgtcccttt aagaaaattt
     4381 acccctatag atatggattt attttgggga atatgttcta aaatgaaaag aaaaggtaca
     4441 aaagaagaaa tttttacatt taaacaaata aaagaattaa ttgattacaa tataaatgca
     4501 cgtggcgacc gttttgctaa tgatttaata aaaatgtcaa aaaaaatcat tactttgtct
     4561 tttatttatg aagacgaaaa aacatatgaa caattaaatc tgtttcaaaa atttaaaatc
     4621 gataaagtga atgaaacatt attaataaag gtttctgaag aatttgaata tatacttaat
     4681 tcaatcggaa ctaattttac tcgtttcgaa ttagaaaata tgacaaactt aaagagtagc
     4741 tacgttaaag agttatatcg ccaacttatg gctcataaag acagaagatc taaaaaaggt
     4801 cactggtttg taaaaataga agattttaga gctctattag caataccaga aagttatcgc
     4861 atgagagata ttgataaaca aatttttgcc caagcgaaaa aagaattttt aaacgttaat
     4921 tcaactcacc ctgctatttt taaaactttt gaaatcaaaa aaattaaagc ctacaaagga
     4981 aataagatag gatctctgca aatttatttt gaagaaaata taccttcaat taccttagat
     5041 aattggttag agaaataata cctaacaatt taggaggcaa ttatcatgca acccaaagaa
     5101 acctttacaa taaaggaatt atctgaatta tttgaaataa gtaggcaagc aatctctaaa
     5161 catgttgata aattagaccc gtccttatta gctaaaaatg agcgtggtta taaggttgta
     5221 ttacgttctg gagtattgca acttgctagc aacttaggta accaaaggtt actatatagg
     5281 ttgcatgagt atgaacaaga aaacgataaa aacgtttata tatcaagcaa aaacgtgatt
     5341 gtaaacgagt tgctagataa gttgcgtgaa gaaaatgaat ttttacgaga acaactttcc
     5401 ctcaaagagc aaaataattc taatatgcaa aaattactcg accagcaaca acagctcacc
     5461 ctacaggcaa atcaacagat cgaaaaactg caagaacagt tacaattagc ttatacggaa
     5521 gaaactccag aagatgaatc aactactatt ctttctgaag aaacagaatc gatagaaaaa
     5581 cacacaaaaa agaagaagca aaaatggtgg caactttgga aataactgga gggaacattc
     5641 atgacaagaa aaaatcttct attcggttct attttactag gaattctagg cttcagtccg
     5701 atgttattgt cagtaatcaa taaagggaat aaacatggtg aattgaaatt gaatccaaat
     5761 tattacaaca gtcctaacga agaattgttt gtaaaagaga cggataaata ataaaaaaga
     5821 ctgctcttcc gagcagccag ttaaaaaata atgagtgttt attcttgaaa aagcacgcgc
     5881 ttatgaagcg ctgccaaaaa tttaccatat tttacaaaaa ataacaagtc ttaaaaaaat
     5941 gacagataaa aatgcacaaa gaattagata tagataaccg cgaaaaatca agctttagaa
     6001 agttgctttt agctagacca attaaaaatc acaaagtgag cagctatagc ccttgataga
     6061 ggggctactt ggcatagtcg acattatgtt aactagctcg ataaataacg attatgttcc
     6121 caaaacaata ttttatgaac tataacaaaa atttgttatc gcatattgta tattttaatt
     6181 atataattat aggtgtatac cacttaaatt taacacaaag acgtgattat tttgaaaccc
     6241 aaaaagaaaa tttatctagg tctcctcttt tccttgatga ttggaatttt tagtatagga
     6301 attacaaata cctatgcttc agaaaattct aatttcaata tcaactccca accaattgaa
     6361 gaacaagcaa ttaattttgc gaaaactgat tttgaacact ccataacttc aattctttta
     6421 atggaaaata acaccgaaac tgatattaac tatactcttg gttcgccatt taaaatgctc
     6481 gatggagaaa atacggcaaa tagttctatt ttctttccag tattttcaga cgctgataat
     6541 acgattaaat atatttatac cgtcaaaaaa aatacaaatg gagaatattc aggaagtatc
     6601 tcacaatttt tagcaaaaga acttcaaaat ctagtagata gtaatataac agatacaacc
     6661 ttattttttc aagataatag catttattat tccgataatg gacaaattaa actactttgg
     6721 aaatcacctt taagtacaga aaaaaatggt gtttcttcct ttacagaaaa aaatgatctt
     6781 atatccgtaa atataaaaaa taaaattaaa aatttctcta aaaaacaaaa t
//
