LOCUS       CP063637                7845 bp    DNA     circular BCT 04-APR-2022
DEFINITION  Enterococcus faecium strain ICU-5-1 plasmid pZA5-1_04, complete
            sequence.
ACCESSION   CP063637
VERSION     CP063637.1
DBLINK      BioProject: PRJNA669626
            BioSample: SAMN16483151
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7845)
  AUTHORS   Both,A., Kruse,F., Mirwald,N., Franke,G., Christner,M., Huang,J.,
            Hansen,J.L., Kroger,N., Berneking,L., Lellek,H., Aepfelbacher,M. and
            Rohde,H.
  TITLE     Population dynamics in colonizing vancomycin-resistant Enterococcus
            faecium isolated from immunosuppressed patients
  JOURNAL   J Glob Antimicrob Resist 28, 267-273 (2022)
   PUBMED   35134550
REFERENCE   2  (bases 1 to 7845)
  AUTHORS   Both,A., Kruse,F., Christner,M., Huang,J., Mirwald,N., Franke,G. and
            Rohde,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) Institute of Medical Microbiology, Virology
            and Hygiene, University Medical Center Hamburg-Eppendorf,
            Martinistr. 52, Hamburg, Hamburg 20246, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 124.1x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/27/2020 18:40:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.13
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 3,095
            CDSs (total)                      :: 3,004
            Genes (coding)                    :: 2,905
            CDSs (with protein)               :: 2,905
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 99
            CDSs (without protein)            :: 99
            Pseudo Genes (ambiguous residues) :: 0 of 99
            Pseudo Genes (frameshifted)       :: 35 of 99
            Pseudo Genes (incomplete)         :: 51 of 99
            Pseudo Genes (internal stop)      :: 25 of 99
            Pseudo Genes (multiple problems)  :: 10 of 99
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="ICU-5-1"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pZA5-1_04"
                     /geo_loc_name="Germany: Hamburg"
                     /lat_lon="53.33 N 10.1 E"
                     /collection_date="2019-03-01"
                     /collected_by="Institute of Medical Microbiology, Virology
                     and Hygiene, University Medical Center Hamburg-Eppendorf"
     gene            complement(join(7480..7845,1..57))
                     /locus_tag="IQN03_15395"
     CDS             complement(join(7480..7845,1..57))
                     /locus_tag="IQN03_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOE17643.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDHS
                     ERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNKKQ
                     LDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
                     /name="hypothetical protein"
     gene            complement(51..1205)
                     /locus_tag="IQN03_15400"
     CDS             complement(51..1205)
                     /locus_tag="IQN03_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="UOE17644.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQPI
                     EEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDA
                     DNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIK
                     LLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDNFIMI
                     NWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKSLNQDQ
                     IIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQ
                     GGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
                     /name="peptidase C47"
     gene            complement(1835..2251)
                     /locus_tag="IQN03_15405"
     CDS             complement(1835..2251)
                     /locus_tag="IQN03_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOE17645.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKIEN
                     QLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(2253..2534)
                     /locus_tag="IQN03_15410"
     CDS             complement(2253..2534)
                     /locus_tag="IQN03_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOE17646.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESDF
                     DRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
                     /name="hypothetical protein"
     gene            complement(2910..3434)
                     /locus_tag="IQN03_15415"
     CDS             complement(2910..3434)
                     /locus_tag="IQN03_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="UOE17647.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINET
                     GQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQEK
                     DEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQS
                     KAEKKWWQFWQ"
                     /name="DUF536 domain-containing protein"
     gene            complement(3451..4419)
                     /locus_tag="IQN03_15420"
     CDS             complement(3451..4419)
                     /locus_tag="IQN03_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UOE17648.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQF
                     TFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKIDGK
                     SDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELLQQLEK
                     LEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
                     /name="replication initiation protein"
     gene            complement(5292..6440)
                     /locus_tag="IQN03_15425"
     CDS             complement(5292..6440)
                     /locus_tag="IQN03_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="UOE17641.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="transposase"
     gene            complement(6457..6861)
                     /locus_tag="IQN03_15430"
     CDS             complement(6457..6861)
                     /locus_tag="IQN03_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="UOE17642.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="IS200/IS605-like element ISEfa4 family transposase"
     Typing          complement(3456..4419)
                     /name="rep_cluster_1093"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.782"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 agttaagaaa agaactaaca ctagacccat aactaaagca atgggtttat ttaccaattt
       61 gtaatggtat tgtcccaagt aaaaccacct ccaggtacag gaattgtaac tggtcctgca
      121 tttgcactta ttacagttgg gtagttatac caaggattcc acacatagta agtttgataa
      181 ccaccttgat tgacccaccc aaataatgca agtgcatgac ggctgttact ataatttcca
      241 taaccagcag ttcccatata aatggcattt ccttttctta gttgtgtttg gacctctgaa
      301 aggtttagta ttcgtgctac ttttttagga taaacaccac agtaatttgc atattttatt
      361 atttgatctt gattaagact ttttgattca atttgactgt tgtttaaatt aggataggta
      421 taatgcataa tggctctagc tgaagtagca cgtacatctg ttttgttaca taagatagca
      481 gcttctacgt aagcagcaca ccacggaaaa cttgtttggg tttctgctat acgccaatta
      541 atcataataa agttatctcc ggaggagaca ctttttacac gtttaaccgc aggaaaattt
      601 tgttttttag agaaattttt aattttattt tttatattta cggatataag atcatttttt
      661 tctgtaaagg aagaaacacc atttttttct gtacttaaag gtgatttcca aagtagttta
      721 atttgtccat tatcggaata ataaatgcta ttatcttgaa aaaataaggt tgtatctgtt
      781 atattactat ctactagatt ttgaagttct tttgctaaaa attgtgagat acttcctgaa
      841 tattctccat ttgtattttt tttgacggta taaatatatt taatcgtatt atcagcgtct
      901 gaaaatactg gaaagaaaat agaactattt gccgtatttt ctccatcgag cattttaaat
      961 ggcgaaccaa gagtatagtt aatatcagtt tcggtgttat tttccattaa aagaattgaa
     1021 gttatggagt gttcaaaatc agttttcgca aaattaattg cttgttcttc aattggttgg
     1081 gagttgatat tgaaattaga attttctgaa gcataggtat ttgtaattcc tatactaaaa
     1141 attccaatca tcaaggaaaa gaggagacct agataaattt tctttttggg tttcaaaata
     1201 atcacgtctt tgtgttaaat ttaagtggta tacacctata attatataat taaaatatac
     1261 aatatgcgat aacaaatttt tgttatagtt cataaaatat tgttttggga acataatcgt
     1321 tatttatcga gctagttaac ataatgtaca ctatgccaag tagcccctct accaatggat
     1381 agagacgctc actttgtgat ttttaattgg tctagctagt tactagcttc taatgcttga
     1441 ttttacgcga tttctctttt ttagttcttt gtgcattttt atctgtcatt ttttaaagac
     1501 ttgttatttt tgagaggatt tgatatgctt tttttgtctt tctctagttc ttcactttgt
     1561 aatattttta tgttgatgaa taggataatc tcattttggg ctgctcgtga gagcagtctt
     1621 tttttgtttc agggtacgtc ctattctttg gatttccgcg ctattaaata accgatactt
     1681 ggggattggg gtagcgcccc aaaatacaaa aagaagaagt tgcataagcg gatttttctt
     1741 ttttttcgca agttggtacc aacttgcaaa tcttgctgga agactgatcc ctaaaaagaa
     1801 atcgaggaat ttttttaggg atcggtttct ttttttatct ttttagaaag tttgttgggt
     1861 caaaatttcc agaagtaaag gtttgattgg tttctttttg acgatttttg ggttgatttc
     1921 gtcgggaagg attgagttga ttttcaattt tttctttcac ttcttgatac actttagaat
     1981 ttgtaccgat aagaacacca gtatctttta gatataaatc agcaaattct gccatgattt
     2041 ctgtcatgat agaaatttct tttcccatgg cattgagttt tgcttggtta ctggttgtgg
     2101 ttgtattggt atcaggttca taactcatac ataaaaaacg aatggcttct gaataattgt
     2161 tgacgtctgg atttttttcg atgatggaat ctaaaacgtc aacgtgactg ggataaaggg
     2221 ttacgtgttt ttgaacagaa gtttttctca tgttatattc ctccgataag tgtttcaatg
     2281 agttggttgt ttgtgtgaac atagtcattc agtatgttcg tattttctgt ttgaatggca
     2341 ataaattttt cgagtaataa atcgaattga tcttttttgt aattcataag ttgacggtca
     2401 aaatcacttt cgattagaac ttttagatat ttatttcgac tccatttttt gcccgttttg
     2461 gttgtgagtt cctgacaacg tttatctatt cttgcaataa ttttgggatt gatatttcga
     2521 actaaaatgt ccatggaaat tttcctttcg tatcagcgta taatttatgt ataccataca
     2581 aatatagtat ttgtatagct atttttcgcc aaaacactat acatagccta gccacaatgg
     2641 caaaaaagtg atagcaaatt tttttctgag aaacgtgata ggcacagcct atctagcacc
     2701 tttcgtaaga aagtgatacc acgtttcctt atgctctttc aaaattcaga aaaatcagct
     2761 gtaaaacgag tgcagtccga gtaagattca acgaaaaaac ggggattggg gcgccaccaa
     2821 aatatcgcaa catgggtcca tgttgctctg ctcgcaaaag caaaaagaag aataaaacca
     2881 gcaattttta ttaatttaaa ttgctggttt tattgccaga attgccacca ttttttctcc
     2941 gcctttgatt gtttttctat cgattctgtt tcttcagaaa gagtcgttga tttattttcc
     3001 gaagattctt ccgtataagt caactgtagt tgttcctgca gtttctcgat ttgttggttg
     3061 gcttgcaggg tgagttgttg ttggtggtcg agtaattttt gcatttgttt tatttgctcg
     3121 tctttttctt gtagttgcat atctttttga tttaactgtt gttctaggag agaaaggact
     3181 tctaattcgg gaacttggtt ggcgtttttg tggttttcgg ttttcgtttt ttcgccaact
     3241 agttgttgat ttgagttttc gttttcgtta aaaaacattg attttatagc cttttgtccg
     3301 gtttcattaa tcacaaactt gtttccgatt ttcgaaaacc aaagttttcg attttcgtca
     3361 gaaacttttt tgttaattgc tgttttacta actcctaatt cttcagcaag ttctttgatt
     3421 gttttttctg tcataaaatc taaaaacccc tcaatctttg aataagtttt gaagattgtc
     3481 taataagtct ttttttagtt ctgaaggaat atcggaattt atctctgatt tttgtgcatt
     3541 attttcttta ttttgggtat tttgttctct ttctaacttt tctaactgtt gtaaaagttc
     3601 caattttcgc ttttgttctt tgattttttc ttgtaatgaa gtaggtgctt tttcagattg
     3661 aacttgttgg ttagctagtt ctaatgcttt gttttgttct tcttttgttg gggcatgttt
     3721 gttaaagtcg tctgattttt ttatctcagg tttaaatgta aattgatagc caatgacagg
     3781 ctttccccgt ccttttccgt atttcttttt gattgttaaa cctttaaaga ttgcggttag
     3841 ttcttgtcgt attggattga acacacgttg atcaatcgta ccttggttgt aacttttagg
     3901 aatatcaagt aattcatgaa agtcttcttt tgaaaaataa gcgaaacctt tggttctgta
     3961 ttgttttagt aatcgaaaca ttgtttttga ataactgctt tctaattcat tgaattgttg
     4021 gagagaatag cgcacccatt cttcaagatt attaagtaag ggtaaggctt tttcatgaac
     4081 ttgtatttca gcaaaaggaa tatcagattt tccgtcgatt ttaaattgat taaataaaac
     4141 aaaacgttca cgttgcaaac cgtcagcact tcttctacca aaacgtaaat ccattaattt
     4201 gtcatacgtt gtttctaaat catctatgaa tcgaacattt gcagttgctt tataattgct
     4261 taaatctttt aattgctcga atgtaaattg tactttttta tcacctacat cacgcattct
     4321 tgatacgata gaaaagaaca aattcatttc gactggtgta aattttcgca acggaattgt
     4381 atttaattca tgatgatact ttacgatttc attagacata tttaaaatag ctcctttctt
     4441 actcgcacaa gagatacacg ttatgaaata caaaatgcct gtttatctac atcttatatt
     4501 aacatcgtcg atgtactaat tcaatgtaca aacagacaaa acgatgtaga taaacagaca
     4561 aaacgatgta gataaacaga caaaacgatg tagataaaca gacaaaacga tgtagataaa
     4621 agcgtcattc ccttgggaga gtaaggatat agccgacggt aaagaggttt aaaaagaggt
     4681 ttaaaaagag attataaaag agggggacaa aatttcccgc ctaaaaaacc acttcttttt
     4741 tctccaaaca gattcgcgca tttttctcaa ctgggaatct tactccctac agtcgcttaa
     4801 aaaggtgtga tcgctctgct ctcacattat actgcgcagt cgcttccggc tcctttgcta
     4861 aaaatgttca taaggaactc tttgagaagc tatgagcctt cgtttgagac gttttaagac
     4921 agtcttagta taaatccctt atcttgttct taaagcgcct tacaggcggt cagggcaagc
     4981 cctaacaacc ctaaagttgt ctgaatgccc tctaaaattc gatttaagca ctcttaaacc
     5041 tactcttata tatttatata cttagacgtt tcaaacgtct taaaacgcaa attagagctt
     5101 gataaagata tttgctcgtg tgccgacttt tgtgaactac tcaccactta gctaaacctg
     5161 acggttctta acgcttgaag tgggagcttc ttgggaatag agcatacttg atagcgtatt
     5221 tctttactaa cagcggtgtc tcttatttac caagctatcc ccgtagttcc tacggttctt
     5281 gatgattgga tctaagccaa tcccatagaa tgtagtctta gaccttcggt cagaatattg
     5341 atactagcat tgatatcacg atcatgatga gtatgacaaa caggacaagt ccattctcga
     5401 atttcaagag atttcttgcc atctttatgt ccgcactctg aacaaatctg actagacgga
     5461 aaccatttat ctactttgat gatttttcgt ccataccagt cagccttgta ttgtaattta
     5521 gtcacaaact ttgaccaaga aacatcagaa atacttttag ctaatttatg atttcgcaac
     5581 ataccttttg tgtttaagtc ttcaatacag ataatatcgt gatttttgat catttctgta
     5641 ctcaacttat tcagaaaatc agtacgttga ttcattactt tttcatgcaa tctagctact
     5701 ttgcgttttt gtttttgata atttttagct tcaaatagat tggtaccttt atttttagcc
     5761 aataaagcac gcctagacaa tttgcgttgt tcacgcttta gtttcttttc cattttggac
     5821 gtgaatttat tattatcaat tttttgtcca tcagaaagaa tcgcaaagtc cataatacct
     5881 aagtcaatac caattgtgga attagtttta ggtaattcga taatttcttc tttacacaac
     5941 aaagaaatat agtatttacc gctagaatga cgtgatattg tagcggattt gataatccct
     6001 tttggttgtc tatgaagctt aattcttact aatgacttca atttaggaac tttgatgaat
     6061 ttactatcaa tcaaagcaac tgtaccattt tgattattcg ttgtataact ctgaacagga
     6121 tttttcttac ttttgaaacg tggaaatcca acggatttat cccgaaaaaa attcttgtat
     6181 gctttatcta aattaagttg ggcattggct aaagcaaggc tatcaatttc tttcaaaaac
     6241 ggaaattctt tcttgtattt agctggtgtc ggaaaagtca tttttttaga agaatcattt
     6301 ttaacttctt catacgcctt ttttcggtca tcaagcatta gattgtagac cttacggaca
     6361 caaccaaaag atttggcaaa gaagatttct tgttcctctg ttggatagat tctgaatttg
     6421 tatgctttta gttgttccat aaagttcacc tactttctat ttatgattgt ttccttgatt
     6481 ttggatatac ttcttaatca catcaattgg tgcgccacca gttgtcagaa gacaaaaact
     6541 tttagaccag aacatttctt tccaaagaaa ttgtttgact cttggaaagt cacgttttat
     6601 caatcttgag ctggcacttt tataggcatt gatgaatttt gtcaattctg ttttaggttg
     6661 agctttgaac ataatatgaa cgtggtcttt atcatgattc cactccacta aagtaatgtg
     6721 atacgattct gaaattcttt caaaagtagt tttggcataa tcagatattt catcatcaat
     6781 cacttgtcta cgatatttcg ttactaaaac aaggtggtaa taaagaagaa atactgaatg
     6841 attatttgta tctaattcca tgttatatca actcatttct tgtatagact gattgtaaca
     6901 tggaacaaaa taaaaaggca atacgcctta gttgtcggtc ttcgagttac caacggtaac
     6961 cctcataccg aacggcattc atcacccacc tatagaggat gggcgacttc tgcctaattc
     7021 tgttaaaagt ctaggcacac gccatgacaa agctgaaata aactgcttca gctttgtcat
     7081 ggcggattcg ctcacttgaa aaagtgttca tttcgctcga ttattagcat atatttttta
     7141 actttttttg ctagccaaat tttgcattag ttcgctcata aatcccaccc tttcagtacg
     7201 tcctacggac gtatcgttcg taatgtgaaa gtacctttaa aggtactttc tacaaaactc
     7261 actaaagtgt ttggcaagct ctatatttgc tagccaaata cctacgtatt tggtgcaaat
     7321 aaccgcttgt caaaggggcg caagctcccc cttgaatccc cctcaaaaac cacgctccac
     7381 aatacaaaaa aggctagcaa atgctagcca ctttttgtaa agtttcgctt gtttcacctt
     7441 cggtgaaatt attgttttgt aatatcaaac aactattcat caatcagctt taaaaaattc
     7501 aattggttta gctgtccctt ctgtcccatc attcgttgtc aaaataaata cgagcttttt
     7561 tggagaatcc aaactaaaag tgtaatctaa ttgtttttta ttagagcgca gtgttagtaa
     7621 attatttgtt gctttgtaaa tgaaaacttg ttcatttata tttaaatttt tatcaatgat
     7681 ttgaatttta tgattgttct cttttactaa gatggtatat tgttctatat tttttgaagt
     7741 atttaaatta gtgattacta gacgttctga atgatcttta gtttgccaag ttcctagaaa
     7801 aggaatcaga tcagtctttt gtatttcttg tttttttgaa caact
//
