LOCUS       CP063628               18637 bp    DNA     circular BCT 04-APR-2022
DEFINITION  Enterococcus faecium strain ICU-4-1 plasmid pZA4-1_02, complete
            sequence.
ACCESSION   CP063628
VERSION     CP063628.1
DBLINK      BioProject: PRJNA669626
            BioSample: SAMN16483152
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18637)
  AUTHORS   Both,A., Kruse,F., Mirwald,N., Franke,G., Christner,M., Huang,J.,
            Hansen,J.L., Kroger,N., Berneking,L., Lellek,H., Aepfelbacher,M. and
            Rohde,H.
  TITLE     Population dynamics in colonizing vancomycin-resistant Enterococcus
            faecium isolated from immunosuppressed patients
  JOURNAL   J Glob Antimicrob Resist 28, 267-273 (2022)
   PUBMED   35134550
REFERENCE   2  (bases 1 to 18637)
  AUTHORS   Both,A., Kruse,F., Christner,M., Huang,J., Mirwald,N., Franke,G. and
            Rohde,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) Institute of Medical Microbiology, Virology
            and Hygiene, University Medical Center Hamburg-Eppendorf,
            Martinistr. 52, Hamburg, Hamburg 20246, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 414.3x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/27/2020 18:33:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.13
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 3,157
            CDSs (total)                      :: 3,064
            Genes (coding)                    :: 2,972
            CDSs (with protein)               :: 2,972
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 27 of 92
            Pseudo Genes (incomplete)         :: 53 of 92
            Pseudo Genes (internal stop)      :: 20 of 92
            Pseudo Genes (multiple problems)  :: 7 of 92
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..18637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="ICU-4-1"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pZA4-1_02"
                     /geo_loc_name="Germany: Hamburg"
                     /lat_lon="53.33 N 10.1 E"
                     /collection_date="2019-03-01"
                     /collected_by="Institute of Medical Microbiology, Virology
                     and Hygiene, University Medical Center Hamburg-Eppendorf"
     gene            join(17507..18637,1..165)
                     /locus_tag="IQN04_15475"
     CDS             join(17507..18637,1..165)
                     /locus_tag="IQN04_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="UOE14704.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHTC
                     RNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSY
                     FVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRIL
                     PRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCTNPEEVKFL
                     VTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKL
                     KKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAYPFFHEL
                     VEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNGKIEAKNT
                     HIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
                     /name="ISL3 family transposase"
     gene            complement(191..865)
                     /locus_tag="IQN04_15480"
     CDS             complement(191..865)
                     /locus_tag="IQN04_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="UOE14705.1"
                     /translation="MNPFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGSLSIRTDELCKIKSE
                     SE"
                     /name="IS6 family transposase"
     gene            complement(1227..1510)
                     /locus_tag="IQN04_15485"
                     /pseudo=""
     CDS             complement(1227..1510)
                     /locus_tag="IQN04_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(1531..2382)
                     /locus_tag="IQN04_15490"
     CDS             complement(1531..2382)
                     /locus_tag="IQN04_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOE14706.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILDL
                     TVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAGKL
                     FKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILFFVG
                     YVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKGIRSI
                     LKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDLELPMK
                     DK"
                     /name="hypothetical protein"
     gene            complement(2483..2779)
                     /locus_tag="IQN04_15495"
     CDS             complement(2483..2779)
                     /locus_tag="IQN04_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="UOE14707.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTRE
                     KTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
                     /name="type III secretion system protein PrgN"
     gene            complement(2914..4407)
                     /locus_tag="IQN04_15500"
     CDS             complement(2914..4407)
                     /locus_tag="IQN04_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="UOE14708.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSKQ
                     LMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINTFV
                     VDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVIDVA
                     KKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSMKLES
                     DQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTLSLAYY
                     SSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELCQQWVSP
                     TLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLNLSKKELA
                     SQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLSKEKRLDYS
                     TRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
                     /name="primase C-terminal domain-containing protein"
     gene            5019..5972
                     /locus_tag="IQN04_15505"
     CDS             5019..5972
                     /locus_tag="IQN04_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="UOE14709.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLFE
                     KDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGSPG
                     SEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAADYII
                     AMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQKTID
                     KYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYYEQHGD
                     IEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
                     /name="ParA family protein"
     gene            5944..6219
                     /locus_tag="IQN04_15510"
     CDS             5944..6219
                     /locus_tag="IQN04_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="UOE14710.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVDP
                     PVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
                     /name="type III secretion system protein PrgO"
     gene            complement(7123..7293)
                     /locus_tag="IQN04_15515"
     CDS             complement(7123..7293)
                     /locus_tag="IQN04_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOE14711.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAAA
                     GYHKYEYYIGY"
                     /name="hypothetical protein"
     gene            complement(7290..7640)
                     /locus_tag="IQN04_15520"
     CDS             complement(7290..7640)
                     /locus_tag="IQN04_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOE14712.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTEE
                     VAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIRHI
                     EFYQDNKWGRLE"
                     /name="hypothetical protein"
     gene            complement(7633..8958)
                     /locus_tag="IQN04_15525"
     CDS             complement(7633..8958)
                     /locus_tag="IQN04_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="UOE14713.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRAD
                     NTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNIFR
                     RYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLATVG
                     VGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRLNQIG
                     IMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQVLPRD
                     YTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKIPITDNT
                     KELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEENLDKIIDK
                     IRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
                     /name="Y-family DNA polymerase"
     gene            9475..10290
                     /locus_tag="IQN04_15530"
     CDS             9475..10290
                     /locus_tag="IQN04_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOE14695.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELEK
                     YLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIARI
                     DDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEADENY
                     NVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDLEALL
                     SANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
                     /name="hypothetical protein"
     gene            10416..10673
                     /locus_tag="IQN04_15535"
     CDS             10416..10673
                     /locus_tag="IQN04_15535"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOE14715.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIFM
                     IVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
                     /name="hypothetical protein"
     gene            complement(10925..11497)
                     /locus_tag="IQN04_15540"
     CDS             complement(10925..11497)
                     /locus_tag="IQN04_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="UOE14696.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRDR
                     PKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTSMG
                     RMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELLTSG
                     KSYKEVEAITGFSRSTLFRIKKKIEES"
                     /name="recombinase family protein"
     gene            complement(11513..12118)
                     /locus_tag="IQN04_15545"
     CDS             complement(11513..12118)
                     /locus_tag="IQN04_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="UOE14697.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTFK
                     GLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEIIKK
                     YVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDLEIR
                     YLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
                     /name="cell filamentation protein"
     gene            complement(12751..13431)
                     /locus_tag="IQN04_15550"
     CDS             complement(12751..13431)
                     /locus_tag="IQN04_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="UOE14698.1"
                     /translation="MNPFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            13578..13661
                     /locus_tag="IQN04_15555"
     CDS             13578..13661
                     /locus_tag="IQN04_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="UOE14714.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
                     /name="23S rRNA methyltransferase attenuation leader
                     peptide"
     gene            13786..14523
                     /gene="erm(B)"
                     /locus_tag="IQN04_15560"
     ARG             13787..14520
                     /name="erm(B)"
                     /gene="erm(B)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(14786..15649)
                     /locus_tag="IQN04_15565"
     CDS             complement(14786..15649)
                     /locus_tag="IQN04_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="UOE14700.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSG
                     KTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEALIS
                     RLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYETMY
                     ADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISP
                     KETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTPPIPKT
                     PKLPGI"
                     /name="zeta toxin family protein"
     gene            complement(15651..15923)
                     /locus_tag="IQN04_15570"
     CDS             complement(15651..15923)
                     /locus_tag="IQN04_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="UOE14701.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVN
                     LLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
                     /name="antitoxin"
     gene            complement(15940..16149)
                     /locus_tag="IQN04_15575"
     CDS             complement(15940..16149)
                     /locus_tag="IQN04_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="UOE14702.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAKN
                     GGNVKEVMDQALEEYIRKYLPDKL"
                     /name="peptide-binding protein"
     gene            complement(16248..17144)
                     /locus_tag="IQN04_15580"
     CDS             complement(16248..17144)
                     /locus_tag="IQN04_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="UOE14703.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYFK
                     GGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKNGN
                     LASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIIIDTV
                     PTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFVPYLV
                     DTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVLSMYEN
                     VFFEMIERIIQLENEKE"
                     /name="ParA family protein"
     gene            complement(17300..17374)
                     /locus_tag="IQN04_15585"
                     /pseudo=""
     CDS             complement(17300..17374)
                     /locus_tag="IQN04_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339851.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /name="IS6 family transposase"
     ARG             13787..14523
                     /name="ErmB"
                     /gene="ErmB"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="98.37"
                     /coverage="98.79"
     Typing          complement(2915..4407)
                     /name="rep_cluster_185"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.866"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gaagaaggca tcaccaacgc aatgatctat ccttattcca atggaaaaat agaagcgaag
       61 aatacccaca taaagacaat gaaacgagta tcctacggat ttaaatcatt tgagaacatg
      121 agaattagaa tctttttgat caatcaatta atcaatgtaa gataacaaaa aatctgagcc
      181 agaatgtggt tcattctgac tcagatttga ttttgcacaa ctcatcagtc cttattgaca
      241 aagagccttc ttttcgggtt ttcttatata atcctcgaat ggcttccatg cctttaatcg
      301 tggtagaggc agtgcgtaaa cttcgataga atttattgcg tctctttact ggacgatggt
      361 cttgttcaat caaattattc aggtatttaa tggtacgatg ttctgttcct tgataaaagc
      421 cgtattcttt tagtttctta aaggcacttg taatagaggg ggctttatct gtgactacaa
      481 ccttcggttc atcaaactgc ttcactaacc gcttaagaaa agcataggct gcttgtgtgt
      541 cccgtttttt acgtaaccaa atatccaagg ttaaaccatc tgcatcgatg gctcgataca
      601 aataatgcca ttttccttta attttgatgt acgtttcatc cattttccat gaataaaagg
      661 attttttatt tttctttttc caaatttgat agagtagttt gccatattct tgcacccaac
      721 gataaatcgt cgtatgagaa acgttaatgc cacgatcata taagatttct tgaacttcac
      781 gatagctaag gttataacga agatagtagc ccacggctac aataatcaca tcctgctgaa
      841 attgctttcc tttaaaagga ttcatcgtca ttcctcctgc tatctttttc tattattcta
      901 ccttatttga tagtagattt aaaactttgc aacagaaccg ttcgtcacat cttcgatcaa
      961 tttctcttac gattttctca tccacatttc ttattcttac ttccatatta attcctccaa
     1021 tttaaaaagg atagcgaaaa ggagcctatc ctttttgcta tcctactacc ctccttttac
     1081 tatccttcac tatcctttca ctatcctatt tttaaatatt ttgttcccac atttattttt
     1141 gagaaacgta ataggcacag cctattggaa cacttttttt aaaaagtgtt cccacgtttc
     1201 cttatgctct ttacaaataa aggcttttag agacgttctg gaattttaga ttccttgaat
     1261 tgttcccatt catcaatcaa ttggctataa gtagccatta atttttttct tgctcttgtt
     1321 gctttcgaat caacaaaaaa tcagtttgat tcgttaattc taaatattct gttcggtatt
     1381 caatgttcat cgtacccgtt ttactacata gtaactgatt atgacgttct ttcactttga
     1441 ataactgctc cttctttaag gtttttaact ccatgccaac tataagcaca atcctgtata
     1501 gaggaatcat tttcaaacga ttctccataa ttatttatct ttcattggaa gttctaaatc
     1561 actactgttc caaaaaatgt atggtttgtt ttgacgaata ttacttccat ctaaccacaa
     1621 ttttccgatg ccatatcgta ggcttgcttc gacttcataa aaattgtaga tctcttttct
     1681 tcgatctatt gctttcattt cctggccatt ttttaaaata gatctgatgc ccttaacagt
     1741 agattcatgg atactcgtgt gaacgatatc cgtattgatc aattcgtcat atcttccaaa
     1801 atcaacattg tactcataac caagaagtgt cggaatcagg tcaatttcaa tttctttttc
     1861 aattgaacgt ttaataagtt tcgtggatac atacccgaca aaaaagagaa taatacccac
     1921 ttcaaacaaa tacatgtgtc catttacaaa gtgaataaac gtcaataaaa aatccttcac
     1981 tccttttgtt gttcttccag attgttcaat ttgttttatt aaagtcgcta gaattgcatt
     2041 tacaacaatt aaatcgaata ggcttttgaa aagttttcct gctgttaatt tttgcctggt
     2101 aaattgcatg actaattgag taaaaataga caatagcatg acaaaaatca acgtcattag
     2161 cactgtaaaa atgtctgatg ctgcaatcct attgggatca tttatcaagt aataagccgt
     2221 tccaaatact ccaatcgagt aaacggttaa atcaagaata agtcgtgccc cgtaactaca
     2281 aagttttcct aatggccata attgcaatac ccaaaatttc tttcgtttgc tatttctttt
     2341 cagaggaagc caggtgtgtt caatccagtc gtccgatttc atttcgattt cttccctaaa
     2401 ttctttcctt taacgttttc ctttgctact ttagcagcga taggttgctc aatgaaccaa
     2461 ttttttaagt aaataaaaac tactactgga tatacttctt cctttttctt aactcgttag
     2521 ctattttata ctcaatatac tcctgctcta acacacacaa acaatcatat acctgatcca
     2581 tagtctcccc agaagctaag gacaacccat gaagaaactc aatcggcaaa gaagcaactg
     2641 ttttttccct ggtaacccaa gaagacaaag tcccctgctt aaaaccataa agctcacaga
     2701 acacttccac agataatccc aaacgaccaa tgataaacac attaacagga tgtgaataga
     2761 cataattttt taacgacata caacttcctc catgatacat aagactgcat acgcagttat
     2821 aagtatacca cctacacgta gctaggcaaa gggagcaaca cctacagaag tatcatagat
     2881 atattgtata aggcaatttg caccttttat ttttcaacct gtatctactt caaaaagatt
     2941 cacttcttta agagggttca agggggttga taattgtcta aatgtctctt ctactaagct
     3001 ctcgttgagt ccaaatacct ctgtgatcct tgttgaataa tctagtctct tttctttact
     3061 tagttgaata accctcgcga acaacgtttt gactgaagca atgatcattc cgccatttct
     3121 tcctcgtgtt accttgtaga agactttatt ctcctctcgt aactcattta agatcttatc
     3181 aagtgttcgc ttgggcatcg ttagttgtga agccaattct ttttttgaca ggttcaaata
     3241 aggctcacta gcaagacttt tttcgcttag ataagccaat agatcttctt tccattcatt
     3301 tttatgacta tattttcttt gatctcgtgg ctttttaaat ttccaccagc cacgtcgttg
     3361 aatgaacaga tcattatttt ttaaggttgg tgaaacccat tgctggcaca attcaataat
     3421 gaattctctg cttgctgctt gataattacc agaataagca ctacgtacaa tctttactaa
     3481 ttctgcttca gatagcggtt cctgtaaccg ttcgttgaat gtgaacatat tgtattcgca
     3541 agtatccaat gcataacctg aagagtaata agctaaagaa agcgtgaaaa tcacattatt
     3601 tcgaccaaga cgtccttctc cgccgacgat gtttgcttta tgaagtaaca tatcgaacca
     3661 cggttcatct acttgacgaa tttctttttt ctcaggaaag acaattagtt ttcgttctcg
     3721 atcttctttt gttttttgat ctgattcaag cttcatcgac cattgaatcc agtcagcaaa
     3781 agaatactga tagttcattt ctttaaaaac aatattattt tcttgtggga atcgagcaat
     3841 tccgaagtgg ttacatccta gatccactcc ggacaatttt tcagccaact gtagacgaat
     3901 tgtttttgaa attttttttg ctacatcgat caccttgaaa ttgcttttct ttgtgacata
     3961 gcctggtgtc tttagtacaa aataaacttg ataaccatga tctgattcta aaattagcgt
     4021 tggcatgtag cccacttgat ctaaacaggc taaaataatt tcgccttgat ggttctcttt
     4081 tgaatcgata tcaactacaa atgtgttgat ttgacttaag tttttctcag aatgaccttt
     4141 aaccactgtt cgataatcat ccgcataagt accaaagcga taggtattag gtgtccaatg
     4201 cgttagtttc ttttcgttct ctaaaatagc ttcttccgaa gtaatgacca taccacgccc
     4261 aacattcatt aattgcttgc tacggtacgc aaagatagca ccactcctac tggaatcaat
     4321 atgacctgca ggtttcatgg tgctgtttcg atatttgtat gtacgtaagc cgtttttaag
     4381 aatagctgaa taaatttcgc tcaacatcga ttgtaacgtc ctttctcaaa caaaaagaga
     4441 acacagttta gctttaatct cagccatagt atgtgttctc tcttcagtgt ttaagaatat
     4501 ttaatttaag tacactaaat aaacgttctt ctgttgagtt gctcgccttt ttttggtaga
     4561 ataaaggcaa tcggaaaagg tatccgatca acttttaata taagtagttg gtagctactt
     4621 acatattctt gagacgagcg tgaacacatg tcctaaccat gtgattcaga aataattgat
     4681 tctgctaaaa tcaattattt ccgtctcttt tttttatgta atttttctgt ttaaagagta
     4741 ccactgcttt tgctactttg taaatagtga attatctgtg gtttctatag aattaagttt
     4801 acctcttttc actctaaatt gtcaacgcaa aacgcacaaa tatattttga cattttgaat
     4861 atatctgata tactgaatat agcaaatata tttagtatat ccactatatt ccttataaag
     4921 tctgaattct tatcaatttg gagtatatta gatatatcga atatattaag tatatgaagt
     4981 atactaaata tatttagtat aggaaattgg agggaactat ggaaaactta cggactgcat
     5041 ttaaaacatc agataaacta ggaaaagttg taatgcttac aggtagaaaa ggcggcattg
     5101 gtaagactac agacaatgat ttattagcga ttgtttctag tcaacttttt gaaaaagacg
     5161 tgttgctaat tgattatgac caacaaagaa atactacttc caacattgga tcaacgtatc
     5221 aaattactag ctttgatcga tccatgtctg cagcaatcaa aaaaggggac tgggtttcag
     5281 gtattactca agtaagtcca catttatata ttatggctgg ctctcctgga tctgaagaac
     5341 ttaatgaata cttgtccgaa aaatatcctg atcgtcgtaa gagatcattg gccttcatta
     5401 aacctttaga agaattgaga aaaaatttcg attatatctt tatcgactgc ccaccatcaa
     5461 cagataatgt ggttcgtgca ttcttaactg ctgcagatta tattattgca atgcaagaat
     5521 taaaacggta cgcaatggaa ggaacagaag attttattaa taaagtttta gttccgattg
     5581 tcacgaattt tgaagagtct catcttcaaa tcattggaat tttgcctgtt cttttctcag
     5641 tgcgacgttc ctctcaacac gcaaactatc aaaaaacaat tgataagtat ggtgaatcaa
     5701 acatttttaa aagcattgtt aaaggctcgg atcggttaga aatgtatggt gagaatggta
     5761 tccaattaaa tgattatgta gatcgaagat ggtgggctat ttttgcagac atctttacag
     5821 aattagaaga aagaattgaa tactatgaac aacatggtga tattgaagga tttgattata
     5881 cacctaaata tgctgactca atggccaatc atatcctacc tcttggaaag gagattaaac
     5941 taaatggcat tattaccaac gaacaaagat aagaagaaaa ttggtgctga agatatttca
     6001 attaaagatg atattaatgt cgagttaggg gaacgaaagt acactgcgaa agaaagaaaa
     6061 ccagttcaag tagatcctcc cgtactaaaa accattcggg atcttagtta tgcaaaagat
     6121 attcctatgt atgaaatagt caaacttgca atggacgcct atgttgatac tttaaacgac
     6181 gaggaacgga ttatatataa tagacgatca aaagattaaa atatagtaag tatactagat
     6241 atagtaaata tatttagtat acttactata tttttagctt tattaatcct aatccttcaa
     6301 aatatgtgta ttgtttgggg gtatacaaca ttcacaaatt ttattgtttc tagtttttac
     6361 tatactaaat atatttaata tagtgagtat agtaaatata ttggttttta tctacaggta
     6421 atggtggttt taacaaacat atagttacag ttactgaaga acagtccgtt agtcagcagg
     6481 agtaagtaat aaatcgtttg tgagacacta ttttgattga tacgacctct aaccatgtgt
     6541 ttgtttcatt tatgttataa taagaatgta agcatattaa acagcaaact atcacagcag
     6601 cactagactc aatcaaatga agggggatgg tgctaatgaa aaaactacta gcaatttccc
     6661 ttatcctaaa aaggagaatt aatagtgtca aaagaaccta tgtccgcatg gatgcagctc
     6721 gctcttagca tcataaagct gatctctaat tttgtggctt tagacaatag agcaaaggtt
     6781 ctaactacgc aatgtttagt aatacttact tgtagcctac tggttgtaat agtatttctt
     6841 ttacttgcgt tattcgtagt acaccttttg tttttttaaa acaaaagaag cttattcaac
     6901 tatatttagc tgaataagct ggtctcaatg ttaaatcaag tttgttggac gggtttaaca
     6961 ttgctttcta gtgctatttt agttagctct agtatgcttc aagctagtta caacgtccta
     7021 aggttgtaac tagttttttt atatttttta attacctata ggtaatttta tcagaattga
     7081 tacaaaatgt aaaacataaa agaaatataa ttttattgca ctttaatagc caatatagta
     7141 ttcgtattta tggtatcctg ctgcagcttc tacatagaaa gagaagcgtt caacaccaac
     7201 ctcatttatc aaacgctggc cataacccca aatctcttct gatagctgat caaaggttat
     7261 atactttagc tcatattgct ggataatgtt cattctaatc gaccccattt attatcctgg
     7321 taaaattcga tatgacgtat ttcgtcgtag tgaactttgt tttttccaac gtagatcccc
     7381 agctcatcac tacctaagat cttgctaaca atatcttctg gatagtatcc ttcagcgttt
     7441 cgttcttctt tttgaataca tactgatcta ttttttatga ctgcagcgtt caatacttcc
     7501 gcaacctctt cagtcgtcat ttgttctttt gccaaatttt gattggctcg ttgttcattg
     7561 tcggcatcaa ttttttcagt atgatcagat aaataaaaac ctgcccactt taaacgtcca
     7621 cgatcattat aatcaatcat ttttaagccc tcctgcgccg cctgcatggc caccaaccaa
     7681 tgtacttcta gtaatactac gagcgccttc taacatagaa gaagcatgta caatcgacgt
     7741 aaaaccatat ttttgccgaa tcttgtcaat aattttatct aaattttctt cgtcaatttg
     7801 cttttgtgga tcactaaata aatccagctg aagcgaatca gtgtacacaa gcttgctata
     7861 ggttattcca acgtgcctta cttcttgtcc ttcataatac ttatcaaaca agaaaagaca
     7921 gtaattctgc aattcctttg tattatctgt tatagggatc ttcatctggt gtgaaaaacc
     7981 tggtctcgtt tctaagatcg acgtgccaat gtataaatgg acacaacctg ttttacagtt
     8041 atgttgcctg atacgaatag ctacttgttc cgccatttct tttaccacca gttcgatttc
     8101 atttctcctg gtataatctc gtggtaacac ttgtgaatta ccatatgatt tttcctttgt
     8161 cggttctggt gggatcgcta tatctgttcg atcgatcccg ttcgcatgaa aatacagctg
     8221 taagccaatc acacctaaat ggctcttaat agtgtatgga ttccaattag ctaagtcttt
     8281 aatactcatg ataccaattt ggttgagtct ttttttcatt cgagatccaa taccccaaag
     8341 gtctgtcatt tcaggaatat tccatacttt atcaggaaca ttttgatacg tccattcagc
     8401 aaggaatcca tcattatgtt ttgcttcatt gtctaaagca agtttagcta ataagggatt
     8461 atctccgaca cctactgtag caagcaatcc tagctcttct ttaatattct cctggatcat
     8521 ttgagccagc ttctttctcc gttgacttct tgttccttca gtcgtaaaaa gattcattga
     8581 tctggtcact tttaagatcg attcatcgat cgagtaaatc aataaatctt cgtctgctac
     8641 ataccttctg aaaatattgt ttacctgcat atttcgctta atatagagat tcatgcgtgg
     8701 tggaacgaca tgtaacgttt taggaaaagg ttgcggtaga tctcgtggtc gactgacatt
     8761 agaaataccg tacttcttct ttgcagctgg tgaagaagct aaaatcaacc ctgatcctgt
     8821 attatccgct cgactcatga cgactaattc agtcgttaat ggatctaaat tcctttctat
     8881 acattcgaca cttgcgtaaa agcttttcat atcgatcaaa aagtaatcat ttactggctc
     8941 ttttgaataa tccatcataa ataacactcc attcttccac attatacaaa catacgttcg
     9001 tttttgtaaa gaagaatcga ttaaatgtac aaaaaaagaa gcataggtaa gttatttccc
     9061 tacttcttct gttgatttgt aaaatcaaca cattgtatca ctttattaaa tggatattta
     9121 ctattagtct tgtgaaaatt tgaaaataag tgtcgtatta tccagttttg cttagttata
     9181 ttagaaaaaa ataagaaatt caaaaaagaa aaaactttcc ttaaaaacaa aaaataggag
     9241 tattcagttt cttaatagtt ttgtaatagt tgtatgtaga agttttctta atagtttttg
     9301 taataattta ttaatcgtag tttatataac ttatttgtaa gtgactagag aattaaataa
     9361 aaaataatta ctatctagta cgaaattaat tacataggat aatatattaa ggtgctggac
     9421 tacaagtaaa aaatactgta tgatcatgac agattcaaga aaggagcaat aagattggtt
     9481 aagatagcag gactttctaa aggtggaaaa accgtttcac aactttataa acaaaactac
     9541 agagactgga gacagttcag aaaaaacatt aaggattctt actttattct accaacagaa
     9601 cttgaaaaat atttaccgaa taacaaagct attaatttat atctgtatta ctgttttaag
     9661 gcaaagaatg aaggcgggga ttcctggcat tcggtcaata ccatagcaga gtctttagga
     9721 gtgacaacta aaactataaa cacctggaat aaaaccttag ttgatttagg tatgattgca
     9781 cgtatagacg ataaccatct cagtaaatcg acaattttac ttcctctctc tagttactat
     9841 aaattagccc ttgatagttc tttaaaggat gttaccgaga gcactgtcca tgaaattgat
     9901 ggcaacttgg ttgcagttta tcatttgttt gaatggagaa aaaatgaggc tgatgaaaat
     9961 tataacgttc cttataacac actctgttgt gtttttaaaa gaaaaacaca aaaagatact
    10021 atctataaat ttatcttggt ccaagatgag aaaataagta atttcgaatt aagcactcct
    10081 tctagtaaga tttatgacga tgcatataga tttgaatgtc aagatctaga agccctcctt
    10141 tccgcaaata atctaaacaa tatagaacat gaaaactttg ttgtaaacac tagatttaat
    10201 ttagtttctg aaaaggactc agatttgtta gatttgctga tagagcttac taaaaatcta
    10261 gataagaaag aaaacattac tgtactataa ctagcatcgg tccatcatta agtttggact
    10321 attagaacac ttgctattac agaaaaaggt atgctagcca cgcttactat ttattggtat
    10381 caaacaagta tcttaatttg aaaggaagat gatttatggc taatttatat atagagaaca
    10441 aaggagactt gaatgccatt attactttaa tcgtagttat ggtaaaatca ataggtaata
    10501 tcacaatagg aatgctgttt attatattag cagcagtgct gatttttatg atagttattt
    10561 ggttcttaaa tgaaagatct aaagacaatc aacaccgtag ggatatggag ataaaatcaa
    10621 tagagtcaat agattattta tcgataataa cactaaaaaa gccacgttcc taaacgtgac
    10681 ttacgctgaa cgatatgtgt tgtttggtag acgaacaaat cgttcattat aaatatatga
    10741 tggtatccta atatagtaag cgtggctagc atacctttaa ttactttacc ttttacagtt
    10801 accatacaca ttttttgagc agaaatcaag taaataaaaa aatattatat ttcacaacat
    10861 gctaatagac tcatatctag aacgcattaa gtatttacct gtctggttcg ctgagacttc
    10921 ttttttaact ttcttcaatt ttcttcttta ttctaaaaag cgtactgcga ctgaaaccgg
    10981 ttatagcttc aacttcctta tacgatttac ctgaagtaag cagttcatat gcgtgcctct
    11041 tcttcggtgt gattgttgct ttcggacgtc cttctctgaa attaggatta ttctttcttg
    11101 caaacatttt tccttcttgg gtacgttcca caatcatatc acgttccatt tctgctacac
    11161 tcaacaacgt ccgtgtcacc attcgcccca tggacgtatt ttcaatcgtt cctagattca
    11221 aaaccttgat cgttacgtta tcatcgagta aaggttcaat gatctctaat gcttctctcg
    11281 tgttacgagc aaatcgatca agcttcgtaa caactatttc atctccaggc ttcagaccag
    11341 ccaacagctg attgaatttt ggccgatccc gttttgtacc cgtaaatttt tctgaaaaaa
    11401 tattcttctt ctctacgccg ttctcaagca actgttgaat ttgttctttt gtactttgag
    11461 aagcggttga gacacgtgca tatccatatt tcgtcatttg gtccacctac ttttacaaat
    11521 catctatcgt atactcagaa tacccttcat agaaatagct gacatcgatt cctttcatat
    11581 ataactcgcg atcgtcaatt ttatcagtca gagcattgga aattagatag cgtatttcca
    11641 aatcgtttat gggacttcgc tccattgcag aaagatagtc tgctttatcg attaagttcc
    11701 aatctacaac tttttgcaat tcttccttta aaatgagatc cagccaaatt ctagtacttc
    11761 ggccatttcc ttctcgaaat gggtgagcga tattcatctc cacatatttc ttgataatct
    11821 cttcaaaaga agtttgtggc atctgatcaa tatgctgtaa cgaatgttct aaatacataa
    11881 ctggtgcaaa tcgaaaattt ccttttgcaa tattgaccga tcggattttt ccggcaaaat
    11941 cgtaaatatc cgaaaataga aattcatgaa tttcagataa acctttaaat gtaccaactt
    12001 cgatttcatt tatttttcca caatcgtaca attctttcgc tttttgttta ctgagttttt
    12061 cttcaacctt agccaactca acttgattgg ttaatcccaa tttattctct aaaatcatgc
    12121 gaaatctcct taactttatg acataagttt atgaaacact ctcaacccca ttatatcgcg
    12181 attttgttta tgtgtcaaaa actattaagt ttctgacccc cgatagattt ttcaagtgtc
    12241 tctattttgc acagtctagt tgacctttga tacacctcta tatttataga gaatctaaaa
    12301 catcaacatg gcatactcaa aattctaaat attagggata aagaattctc ctatacaatc
    12361 aaaaatttcc tttatttcga actccggtca cctaactcta ttgatctctt acaagccaaa
    12421 gaatatctct atactacaaa gaaacaatag ggtgcctggt tggtctcttt gtgttttatt
    12481 ttagaatcta gaacgtaaat attcttgtca ttctgatttt atctaagaat atcaattaaa
    12541 tctttttttg cagcctctga agcgggcatt acagaaaaga ccagtccaat aaccgaagat
    12601 accccaaccg ctagtaaaat agtaaataga tcaagtgaca cgtttacctt tatcaaagaa
    12661 ccaacactat aagcaagagc cattcctaat aaatagggtt ctgttgcaaa gttttaaata
    12721 aagaataaaa tcccttacgg tatctatgat ttaagctggg attcccaata ataccttgat
    12781 ttcagtacag accgaaaacc cgaagagagt gccttctttt cgggttttct tatataatcc
    12841 tcgaatggct tccatgcctt taatcgtggt agaggcagtg cgtaaacttc gatagaattt
    12901 attgcgtctc tttactggac gatggtcttg ttcaatcaaa ttattcaggt atttaatggt
    12961 acgatgttct gtcccttgat aaaagccgta ttcttttagt ttcttaaagg cacttgtaat
    13021 agagggggct ttatctgtga ctacaacctt cggttcatca aactgcttca ctaaccgctt
    13081 aagaaaagca taggctgctt gtgtgtcccg ttttttacgt aaccaaatat ccaaggttaa
    13141 accatctgca tcgatggctc gatacaaata atgccatttt cctttaattt tgatgtacgt
    13201 ttcatccatt ttccatgaat aaaaggattt tttatttttc tttttccaaa tttgatagag
    13261 tagtttgcca tattcttgca cccaacgata aatcgtcgta tgagaaacgt taatgccacg
    13321 atcatataag atttcttgaa cttcacgata gctaaggtta taacgaagat agtagcccac
    13381 ggctacaata atcacatcct gctgaaattg ctttccttta aaaggattca tcgtcattcc
    13441 tcctgctatc tttttctatt attctacctt atttgatagt agatttaaaa ctttgcaaca
    13501 gaaccaaaat tttgtataat aggaattgaa gttaaattag atgctaaaaa tttgtaatta
    13561 agaaggaggg attcgtcatg ttggtattcc aaatgcgtaa tgtagataaa acatctaccg
    13621 ttttgaaaca gactaaaaac agtgattacg cagataaata aatacgttag attaattcct
    13681 accagtgact aatcttatga ctttttaaac agataactaa aattacaaac aaatcgttta
    13741 acttctgtat ttatttatag atgtaatcac ttcaggagtg attacatgaa caaaaatata
    13801 aaatattctc aaaacttttt aacgagtgaa aaagtactca accaaataat aaaacaattg
    13861 aatttaaaag aaaccgatac cgtttacgaa attggaacag gtaaagggca tttaacgacg
    13921 aaactggcta aaataagtaa acaggtaacg tctattgaat tagacagtca tctattcaac
    13981 ttatcgtcag aaaaattaaa actgaacact cgtgtcactt taattcacca agatattcta
    14041 cagtttcaat tccctaacaa acagaggtat aaaattgttg ggaatattcc ttaccattta
    14101 agcacacaaa ttattaaaaa agtggttttt gaaagccatg cgtctgacat ctatctgatt
    14161 gttgaagaag gattctacaa gcgtaccttg gatattcacc gaacactagg gttgctcttg
    14221 cacactcaag tctcgattca gcaattgctt aagctgccag cggaatgctt tcatcctaaa
    14281 ccaaaagtaa acagtgtctt aataaaactt acccgccata ccacagatgt tccagataaa
    14341 tattggaagc tatatacgta ctttgtttca aaatgggtca atcgagaata tcgtcaactg
    14401 tttactaaaa atcagtttca tcaagcaatg aaacacgcca aagtaaacaa tttaagtacc
    14461 gttacttatg agcaagtatt gtctattttt aatagttatc tattatttaa cgggaggaaa
    14521 taattctatg agtcgctttt gtaaatttgg aaagttacac gttactaaag ggaatgtaga
    14581 taaattatta ggtatagggc acctctaata actaccaatt aattcacctc aaaatgaaat
    14641 tagaaaaaaa cagcgaaatt tttaaatcta tttcttatcg atacaaattc ctcgtaggcg
    14701 ctcgggaccc caacatgagg aaatgttttt ataaaaaaag agtagttacc gttttttggt
    14761 aactactctt ttcctttttt aaggtttaaa tacctggaag tttaggtgtt ttgggtattg
    14821 gtggtgtagg tagctgcaag ctttccattt tttgttgaat tgctttaaat tcaggggttt
    14881 cttggtgttg attttgaacc attttttgct ctattctctc caaagtcggt tgaatttctt
    14941 tccctgatac tttacgattt aattctcttt ctaaggtctc tttcggacta atggaaggcg
    15001 tttctaagct tgaatagagt ttcactcctt ctctattgta aagccttata tcgctaaata
    15061 agcccgtttt atgaagggtt tctaaattgg tcggtaagtt tttgacaaca atatcatgcg
    15121 cttgttttgg tgttgcccta gctgtcattg gatcatctgc atacatggtt tcatatcgtt
    15181 caatcgttcc taagtatgat tcaattttcg gtactgccat gacgtatatt tttgtttcat
    15241 aacctttaga ttgaagcatt gtggctgttt gaatgggaac gtctgttgtt cgtcctgttc
    15301 cttcaatcac taaattatag ccttgatcgc ttaaacggct aataagtgct tctgtcatgc
    15361 gattcgaata aggggtaacg tgttttacta cgtctttttc atagagtttc actaattcgt
    15421 caaaatttgg gtgctgttgt ttaaaggtat cattatcaat gacaataaca ttcccttgtg
    15481 tttcttcaaa aattgctgat cgcaaactgg ttttccctga ccctggttgc ccaccaagta
    15541 aaaaagcggt tggcgattca accgctagtc tatttttagt tagtcgttca acattctttt
    15601 ctaaacgatc ttcaaattgc ttttcggtga agtcagtaat attcgccatg ttaagccact
    15661 ttctctttat tcaaaatttg ttttgaatat tgattcattg accgccaata ctcatgaata
    15721 gcttgtaatt cttcaaatgg ttgttgaaat aaatcaactt cttgtgccac ttctaattga
    15781 tttaaaagat tcacttctag taaacgagta tttttagtat ctagttcatg attcaaaaca
    15841 taatttaata cccgattata aacacttgcc gataactcat taatgatttc aatttcaaat
    15901 gttttttcat acgtaactgc catttcattt tcactccttt taaagtttgt caggtaaata
    15961 tttccgtata tattcttcta aggcttgatc cataacttct tttacgtttc cgccattctt
    16021 tgctgtttcg atttttatga tatggtgcaa gtcggcacga acacgaaccg tcttatcccc
    16081 cattatatct ttttttgcgc tgattggcgt atcatttcgt tttgctttct gtgcgcctaa
    16141 atttcccacg atcgctcact tctttctatt tcttcttact atttatttta tcatcaccta
    16201 tcacaaatca caagtgattt gtgattaatc acttgtgatt tgtgattcta ttctttttca
    16261 ttttctaatt gaatgattcg ctcaatcatt tcaaaaaata cgttctcata catagacaaa
    16321 acttttttgt catagccttt gtgttctgta atgccatttt tagaccaagt acttacttta
    16381 ttacttcgct tgataatatt tcggaaaacc aaattatcct ctttatgttc tttgtacagt
    16441 tcttccaggt ttgattttat cgttgcgctg tccgtatcaa ctaaataagg aacaaaacca
    16501 atcatatcta gtccagggtt aaactgttct tgcaaatcaa tcaaatagga aatatagttt
    16561 tgaatgttgt ttgtactttc ttcttctgct tgtaaaggga tcataacgta atcactcgcc
    16621 acgattgcat tatttgtata aacgcttggc gttggtacag tatcaataat aataagatca
    16681 tagtcacttt ttaaaggtgc taaaagagta gcaagcaatc tactttcatt ttcaaacgtc
    16741 catgagcgag ttaattttgg cagtaacatc aaatcaaacg tgccagggat caagtctaaa
    16801 ttatcagtca aatgaataat agaagaagcc aagtttccat ttttcaagcc ttcataaaaa
    16861 ttgacacgtg gcaattctac ctcaaaagtt tttgctaagt cttttgtcaa tgttgcctgt
    16921 aagtccttat cgatcattaa aacttttaaa ttcaatttgt ctgtcaagta agcaaacata
    16981 gtcgataatt tggactttcc aacaccacct ttaaagtaat tattcaagat aataattgct
    17041 tcatttttat tgtttaaaat acgtcttaat tcttcaagta tttttagttt ttccttctcc
    17101 ataactacgc cccattcttt ttttgtatag taatattgta tcattaagaa tcacaaatca
    17161 caagtgattt gtgattaatc acttgtgatt tgtgataagt gatttgtgat taatatataa
    17221 aagccctctt taaagggctt ttataggttc tgttgcaaag ttttaaataa agaataaaat
    17281 cccttacggt atctatgatt taagctggga ttcccaataa taccttgatt tcagtacaga
    17341 ccgaaaaccc gaagagagtg ccttcttttc ggctctataa tttatgggac tgatgaatca
    17401 gaattgattc ttcagcccca tttttatttt aggtattaaa aaacatatcg ttctctagta
    17461 tgattaaatc acgacaaaat aaacaactga gaggacgata tgctatatga atgattctat
    17521 caaaaaaatg ctgcgattaa tagagaaaga tttgatgatt acagaggtct cttacgagac
    17581 ctttcagaag aaaaagacgt tgatcgtcga tgctgttttc tcacctgctc cccatacctg
    17641 tagaaattgt ggttctactg tggtagatgg aaacgggaaa gtgattgtag tgaaaaatgg
    17701 gaagaaggaa acgattgtcc gctttgaaca atacaatcac atgcctttgg ttatgcgctt
    17761 aaaaaagcag cgctatacct gtaaaaactg tcgaacccat tggacggctc aaagttattt
    17821 tgttcaaccc agacattcaa tcgcgaatca tgtcagatat aaaattgctt ctttactgac
    17881 tgaaaaagta tctttatctt ttattgcgaa aaattgtcag gtatctttga ccaccgttat
    17941 tcgtacgttg aaagagttta aaagctactt accaaagcaa tctaagagga ttctccccag
    18001 agtattgatg gttgatgaat ttcgttcgca cgcttccata gaagataaaa tgagctttat
    18061 ttgcgcagat ggcgaaacag gacaattaat tgatgtcttg cctacgcgta aattgcctcg
    18121 attaacaagc tatttcttag gctgcactaa tcctgaagaa gtaaaatttt tagtcacaga
    18181 catgaacgcc gcctacttcc agcttaccaa acgtattctg ccaaatgcga aagtagtgat
    18241 tgatcggttt catattatca aacacatgaa tcaagccttt aatgagttgc gtatccgtga
    18301 aatgaatgaa cttcgtaaag taggacagaa aagtcaggca gaaaaactga aaaagaactg
    18361 gcgctttttg ctaaaaaatc gtgcaaacat caaccattat gaatacaaaa catggaaaag
    18421 ttttcgagca ccaaaatacc catttcttac ggaagcgatg atgattgatc gattgcttga
    18481 gttttctccg cccctaaagg aggcgtatcc cttttttcat gaattagttg aagcctttcg
    18541 agacaaagac cctgacttat ttttctcctt attggcagaa cttcccgaaa cgttagatga
    18601 cagctttcgg gaaaagcttc aaaaccttct gacctat
//
