LOCUS       CP063622                8270 bp    DNA     circular BCT 04-APR-2022
DEFINITION  Enterococcus faecium strain BMT-4-1-3 plasmid pRL-1-3_04, complete
            sequence.
ACCESSION   CP063622
VERSION     CP063622.1
DBLINK      BioProject: PRJNA669626
            BioSample: SAMN16483153
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8270)
  AUTHORS   Both,A., Kruse,F., Mirwald,N., Franke,G., Christner,M., Huang,J.,
            Hansen,J.L., Kroger,N., Berneking,L., Lellek,H., Aepfelbacher,M. and
            Rohde,H.
  TITLE     Population dynamics in colonizing vancomycin-resistant Enterococcus
            faecium isolated from immunosuppressed patients
  JOURNAL   J Glob Antimicrob Resist 28, 267-273 (2022)
   PUBMED   35134550
REFERENCE   2  (bases 1 to 8270)
  AUTHORS   Both,A., Kruse,F., Christner,M., Huang,J., Mirwald,N., Franke,G. and
            Rohde,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) Institute of Medical Microbiology, Virology
            and Hygiene, University Medical Center Hamburg-Eppendorf,
            Martinistr. 52, Hamburg, Hamburg 20246, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 65.1x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/27/2020 18:03:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.13
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 3,126
            CDSs (total)                      :: 3,033
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 34 of 98
            Pseudo Genes (incomplete)         :: 58 of 98
            Pseudo Genes (internal stop)      :: 17 of 98
            Pseudo Genes (multiple problems)  :: 10 of 98
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="BMT-4-1-3"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pRL-1-3_04"
                     /geo_loc_name="Germany: Hamburg"
                     /lat_lon="53.33 N 10.1 E"
                     /collection_date="2015-01-07"
                     /collected_by="Institute of Medical Microbiology, Virology
                     and Hygiene, University Medical Center Hamburg-Eppendorf"
     gene            complement(join(8199..8270,1..333))
                     /locus_tag="IQN05_15510"
     CDS             complement(join(8199..8270,1..333))
                     /locus_tag="IQN05_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="UOC54430.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="IS200/IS605-like element ISEfa4 family transposase"
     gene            complement(1446..1880)
                     /locus_tag="IQN05_15515"
     CDS             complement(1446..1880)
                     /locus_tag="IQN05_15515"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOC54431.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRYN
                     HNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSAVD
                     AMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
                     /name="hypothetical protein"
     gene            complement(2110..3258)
                     /locus_tag="IQN05_15520"
     CDS             complement(2110..3258)
                     /locus_tag="IQN05_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="UOC54432.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="transposase"
     gene            complement(3275..3679)
                     /locus_tag="IQN05_15525"
     CDS             complement(3275..3679)
                     /locus_tag="IQN05_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="UOC54433.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="IS200/IS605-like element ISEfa4 family transposase"
     gene            complement(4382..4798)
                     /locus_tag="IQN05_15530"
     CDS             complement(4382..4798)
                     /locus_tag="IQN05_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOC54426.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSYE
                     PDTNTITTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKIEN
                     QLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(4800..5081)
                     /locus_tag="IQN05_15535"
     CDS             complement(4800..5081)
                     /locus_tag="IQN05_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOC54427.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESDF
                     DRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
                     /name="hypothetical protein"
     gene            complement(5528..6496)
                     /locus_tag="IQN05_15540"
     CDS             complement(5528..6496)
                     /locus_tag="IQN05_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UOC54428.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQF
                     TFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKIDGK
                     SDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELLQQLEK
                     LEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
                     /name="replication initiation protein"
     gene            complement(7034..8182)
                     /locus_tag="IQN05_15545"
     CDS             complement(7034..8182)
                     /locus_tag="IQN05_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="UOC54429.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="transposase"
     Typing          complement(5533..6496)
                     /name="rep_cluster_1093"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.679"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 aagacaaaaa cttttagacc agaacatttc tttccaaaga aattgtttga ctcttggaaa
       61 gtcacgtttt atcaatcttg agctggcact tttataggca ttgatgaatt ttgtcaattc
      121 tgttttaggt tgagctttga acataatatg aacgtggtct ttatcatgat tccactccac
      181 taaagtaatg tgatacgatt ctgaaattct ttcaaaagta gttttggcat aatcagatat
      241 ttcatcatca atcacttgtc tacgatattt cgttactaaa acaaggtggt aataaagaag
      301 aaatactgaa tgattatttg tatctaattc catgttatat caactcattt cttgtataga
      361 ctgattgtaa catggaacaa aataaaaagg caatacgcct tagttgtcgg tcttcgagtt
      421 accaacggta accctcatac cgaacggcat tcatcaccca cctatagagg atgggcgact
      481 tctgcctaat tctgttaaaa aggtgtgatc gctctgctct cacattatac tgcgcagtcg
      541 cttccggctc ctttgctaaa aatattcata aggaactctt tgagaagcta tgagccttcg
      601 tttgagacgt tttaagacag tcttagtata aatcccttat cttgttctta aagcgcctta
      661 caggcggtca gggcaagccc taacaaccct aaagttgtct gaatgccctc taaaattcga
      721 tttaagcact cttaaaccta ttcttatata tttatatact tagacgtttc aaacgtctta
      781 aaacgcaaat tagagcttga taaagatatt tgctcgtgtg ccgacttttt taaaagtcta
      841 ggcacacgcc atgacaaagc tgaaataaac tgcttcagct ttgtcatggc ggattcgctc
      901 acttgaaaaa gtgttcattt cgctcgatta ttagcatata ttttttaact ttttttgcta
      961 gccaaatttt gcattagttc gctcataaat cccacccttt cagtacgtcc tacggacgta
     1021 tcgttcgtaa tgtgaaagta cctttaaagg tactttctac aaaactcact aaagggtttg
     1081 gcaagctcta tatttgctag ccaaatacct acgtatttgg tgcaaataac cgcttgtcaa
     1141 aggggcgcaa gctccccctt gaatcccccc tcaaaaccca cgctccacaa tacaaaaaag
     1201 gctagcaaat gctagccact ttttgtaaaa tttagtatga tttattttta gctatttaga
     1261 gtttttattt ccgtaaatac ttctaaaaat tgctaagata aatgctatag gaattagata
     1321 aagaatactt ttttgtaagc ttatattgaa aattagccta aatatgaaga acatagtgat
     1381 taaaactatt attgaaataa ttacagctaa aatcttttta ttcatgagat agtttcactt
     1441 tctgattatt ttattacata acgagcgtaa tcattgtatt gatatgatgt tgtattgcta
     1501 aagtgagcat tatatgttaa ttgtacacct tttccttgat ttgcagctct tacaaagaca
     1561 ttcattgcat caacagcact actaaaagca tttgtaataa cccatgttcc aactccacca
     1621 ataggaataa aagatgcaat ttgagcagga gtctttagct tattataatt ttttttcaaa
     1681 tctctacaca ttttattagt aacataatag ttcattttat agcaagtaaa tccacgatga
     1741 ttatgattat atcgtgaact tgcagcttcc acttttatac ctctagcact aaaaggaaac
     1801 agatttaacg gagtacttgc tccaacaatc ataccgaaca aaaatagaca agtaataatt
     1861 ttaataattt tatttttcat tgttacttcc tccttagata taaaatccta ggaaagcgct
     1921 ttctgtatat atttagaata atcagaaaag tgaactactc accacttagc taaacctgac
     1981 ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat agcgtatttc
     2041 tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta cggttcttga
     2101 tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca gaatattgat
     2161 actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc attctcgaat
     2221 ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac tagacggaaa
     2281 ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt gtaatttagt
     2341 cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat ttcgcaacat
     2401 accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca tttctgtact
     2461 caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc tagctacttt
     2521 gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat ttttagccaa
     2581 taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca ttttggacgt
     2641 gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca taatacctaa
     2701 gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt tacacaacaa
     2761 agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga taatcccttt
     2821 tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt tgatgaattt
     2881 actatcaatc aaagcaactg taccattttg attattcgtt gtataactct gaacaggatt
     2941 tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat tcttgtatgc
     3001 tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt tcaaaaacgg
     3061 aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag aatcattttt
     3121 aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct tacggacaca
     3181 accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc tgaatttgta
     3241 tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt ccttgatttt
     3301 ggatatactt cttaatcaca tcaattggtg cgccaccagt tgtcagaaga caaaaacttt
     3361 tagaccagaa catttctttc caaagaaatt gtttgactct tggaaagtca cgttttatca
     3421 atcttgagct ggcactttta taggcattga tgaattttgt caattctgtt ttaggttgag
     3481 ctttgaacat aatatgaacg tggtctttat catgattcca ctccactaaa gtaatgtgat
     3541 acgattctga aattctttca aaagtagttt tggcataatc agatatttca tcatcaatca
     3601 cttgtctacg atatttcgtt actaaaacaa ggtggtaata aagaagaaat actgaatgat
     3661 tatttgtatc taattccatg ttatatcaac tcatttcttg tatagactga ttgtaacatg
     3721 gaacaaaata aaaaggcaat acgccttagt tgtcggtctt cgagttacca acggtaaccc
     3781 tcataccgaa cggcattcat cacccaccta tagaggatgg gcgacttctg cctaattctg
     3841 ttaaatattg tcaagagggt ttattttata aaacgtgtta ttttaaaggt tagtagacat
     3901 aatgtcgact atgccaagta gcccctctat caagggctat agctgctcac tttgtgattt
     3961 ttcattggtc taggtcaaaa gcttttttta aggcttgatt ttctgcagtt gcttctttcc
     4021 gtttctttgt gaatttttac cagtcatttt ttagagattt gttatttttg tgagcatttg
     4081 atatgctctt tttatacctc aatctcttct tttctaatgt taatgaatag gataatctca
     4141 tttttagctg ctcgtgagag cagtcttttt ttgtttcagg gtacgtccta ttctttggat
     4201 ttccgcgcta ttaaataacc gatacttggg gattggggta gcgccccaaa atacaaaaag
     4261 aagaagttgc ataagcggat ttttcttttt tttcgcaagt tggtaccaac ttgcaaatct
     4321 tgctggaaga ctgatcccta aaaagaaatc gaggaatttt tttagggatc ggtttctttt
     4381 tttatctttt tagaaagttt gttgggtcaa aatttccaga agtaaaggtt tgattggttt
     4441 ctttttgacg atttttgggt tgatttcgtc gggaaggatt gagttgattt tcaatttttt
     4501 ctttcacttc ttgatacact ttagaatttg taccgataag aacaccagta tcttttagat
     4561 ataaatcagc aaattctgcc atgatttctg tcatgataga aatttctttt cccatggcat
     4621 tgagttttgc ttggttactg gttgtgattg tattggtatc aggttcataa ctcatacata
     4681 aaaaacgaat ggcttctgaa taattgttga cgtctggatt tttttcgatg atggaatcta
     4741 aaacgtcaac gtgactggga taaagggtta cgtgtttttg aacagaagtt tttctcatgt
     4801 tatattcctc cgataagtgt ttcaatgagt tggttgtttg tgtgaacata gtcattcagt
     4861 atgttcgtat tttctgtttg aatggcaata aatttttcga gtaataaatc gaattgatct
     4921 tttttgtaat tcataagttg acggtcaaaa tcactttcga ttagaacttt tagatattta
     4981 tttcgactcc attttttgcc cgttttggtt gtgagttcct gacaacgttt atctattctt
     5041 gcaataattt tgggattgat atttcgaact aaaatgtcca tggaaatttt cctttcgtat
     5101 cagcgtataa tttatgtata tcatacaaat atagtatttg tatagctatt tttcgccaaa
     5161 acactataca tagcctagcc acaatggcaa aaaagtgata gcaaattttt ttctgagaaa
     5221 cgtgataggc acagcctatc tagcaccttt tgtaagaaag tgataccacg tttccttatg
     5281 ctctttcaaa attcagaaaa atcagctgta aaacgagtgc agtccgagta agattcaacg
     5341 aaaaaacggg gattggggcg ccaccaaaat atcgcaacat gggtccatgt tgctctgctc
     5401 gcaaaagcaa aaagaagaat aaaaccagca atttaaatta ataaaaattg ctggttttat
     5461 tgccagaatt gccaccattt tttctccgtc tttgattgtt ttttctgtca taaaatctaa
     5521 aaacccctca atctttgaat aagttttgaa gattgtctaa taagtctttt tttagttctg
     5581 aaggaatatc ggaatttatc tctgattttt gtgcattatt ttctttattt tgggtatttt
     5641 gttctctttc taacttttct aactgttgta aaagttccaa ttttcgcttt tgttctttga
     5701 ttttttcttg taatgaagta ggtgcttttt cagattgaac ttgttggtta gctagttcta
     5761 atgctttgtt ttgttcttct tttgttgggg catgtttgtt aaagtcgtct gattttttta
     5821 tctcaggttt aaatgtaaat tgatagccaa tgacaggctt tccccgtcct tttccgtatt
     5881 tctttttgat tgttaaacct ttaaagattg cagttagttc ttgtcgtatt ggattgaaca
     5941 cacgttgatc aatcgtacct tggttgtaac ttttaggaat atcaagtaat tcatgaaagt
     6001 cttcttttga aaaataagcg aaacctttgg ttctgtattg ttttagtaat cgaaacattg
     6061 tttttgaata actgctttct aattcattga attgttggag agaatagcgc acccattctt
     6121 caagattatt aagtaagggt aaggcttttt catgaacttg tatttcagca aaaggaatat
     6181 cagattttcc gtcgatttta aattgattaa ataaaacaaa acgttcacgt tgcaaaccgt
     6241 cagcacttct tctaccaaaa cgtaaatcca ttaatttgtc atacgttgtt tctaaatcat
     6301 ctatgaatcg aacatttgca gttgctttat aattgcttaa atcttttaat tgctcgaatg
     6361 taaattgtac ttttttatca cctacatcac gcattcttga tacgatagaa aagaacaaat
     6421 tcatttcgac tggtgtaaat tttcgcaacg gaattgtatt taattcatga tgatacttta
     6481 cgatttcatt agacatattt aaaatagctc ctttcttact cgcacaagag atacacgtta
     6541 tgaaatacaa aatgcctatt tatctacatc ttatattaac atcgtcgatg tactaattca
     6601 atgtacaaac agacaaaacg atgtagataa acagacaaaa cgatgtagat aaacagacaa
     6661 aacgatgtag ataaacagac aaaacgatgt agataaaagc gtcattccct tgggagagta
     6721 aggatatagc cgacggtaaa gaggtttaaa aagaggttta aaaagagatt ataaaagagg
     6781 gggacaaaat ttcccgccta aaaaaccact tcttttttct ccaaacagat tcgcgcattt
     6841 ttctcaactg ggaatcttac tccctacagt cgcgtgaact actcaccact tagctaaacc
     6901 tgacggttct taacgcttga agtgggagct tcttgggaat agagcatact tgatagcgta
     6961 tttctttact aacagcggtg tctcttattt accaagctat ccccgtagtt cctacggttc
     7021 ttgatgattg gatctaagcc aatcccatag aatgtagtct tagaccttcg gtcagaatat
     7081 tgatactagc attgatatca cgatcatgat gagtatgaca aacaggacaa gtccattctc
     7141 gaatttcaag agatttcttg ccatctttat gtccgcactc tgaacaaatc tgactagacg
     7201 gaaaccattt atctactttg atgatttttc gtccatacca gtcagccttg tattgtaatt
     7261 tagtcacaaa ctttgaccaa gaaacatcag aaatactttt agctaattta tgatttcgca
     7321 acataccttt tgtgtttaag tcttcaatac agataatatc gtgatttttg atcatttctg
     7381 tactcaactt attcagaaaa tcagtacgtt gattcattac tttttcatgc aatctagcta
     7441 ctttgcgttt ttgtttttga taatttttag cttcaaatag attggtacct ttatttttag
     7501 ccaataaagc acgcctagac aatttgcgtt gttcacgctt tagtttcttt tccattttgg
     7561 acgtgaattt attattatca attttttgtc catcagaaag aatcgcaaag tccataatac
     7621 ctaagtcaat accaattgtg gaattagttt taggtaattc gataatttct tctttacaca
     7681 acaaagaaat atagtattta ccgctagaat gacgtgatat tgtagcagat ttgataatcc
     7741 cttttggttg tctatgaagc ttaattctta ctaatgactt caatttagga actttgatga
     7801 atttactatc aatcaaagca actgtaccat tttgattatt cgttgtataa ctctgaacag
     7861 gatttttctt acttttgaaa cgtggaaatc caacggattt atcccgaaaa aaattcttgt
     7921 atgctttatc taaattaagt tgggcattgg ctaaagcaag gctatcaatt tctttcaaaa
     7981 acggaaattc tttcttgtat ttagctggtg tcggaaaagt cattttttta gaagaatcat
     8041 ttttaacttc ttcatacgcc ttttttcggt catcaagcat tagattgtag accttacgga
     8101 cacaaccaaa agatttggca aagaagattt cttgttcctc tgttggatag attctgaatt
     8161 tgtatgcttt tagttgttcc ataaagttca cctactttct atttatgatt gtttccttga
     8221 ttttggatat acttcttaat cacatcaatt ggtgcgccac cagttgtcag
//
