LOCUS       CP063609               20325 bp    DNA     circular BCT 04-APR-2022
DEFINITION  Enterococcus faecium strain BMT-2-3-8 plasmid pSS-3-8_05, complete
            sequence.
ACCESSION   CP063609
VERSION     CP063609.1
DBLINK      BioProject: PRJNA669626
            BioSample: SAMN16483156
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20325)
  AUTHORS   Both,A., Kruse,F., Mirwald,N., Franke,G., Christner,M., Huang,J.,
            Hansen,J.L., Kroger,N., Berneking,L., Lellek,H., Aepfelbacher,M. and
            Rohde,H.
  TITLE     Population dynamics in colonizing vancomycin-resistant Enterococcus
            faecium isolated from immunosuppressed patients
  JOURNAL   J Glob Antimicrob Resist 28, 267-273 (2022)
   PUBMED   35134550
REFERENCE   2  (bases 1 to 20325)
  AUTHORS   Both,A., Kruse,F., Christner,M., Huang,J., Mirwald,N., Franke,G. and
            Rohde,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) Institute of Medical Microbiology, Virology
            and Hygiene, University Medical Center Hamburg-Eppendorf,
            Martinistr. 52, Hamburg, Hamburg 20246, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Trycycler v. v0.3.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.5x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/27/2020 17:58:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.13
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,079
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 40 of 111
            Pseudo Genes (incomplete)         :: 66 of 111
            Pseudo Genes (internal stop)      :: 13 of 111
            Pseudo Genes (multiple problems)  :: 7 of 111
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..20325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="BMT-2-3-8"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pSS-3-8_05"
                     /geo_loc_name="Germany: Hamburg"
                     /lat_lon="53.33 N 10.1 E"
                     /collection_date="2014-07-23"
                     /collected_by="Institute of Medical Microbiology, Virology
                     and Hygiene, University Medical Center Hamburg-Eppendorf"
     gene            join(20115..20325,1..1352)
                     /locus_tag="IQN08_15695"
     CDS             join(20115..20325,1..1352)
                     /locus_tag="IQN08_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="UOC45543.1"
                     /translation="MTEKNIMVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSS
                     YEVQVSHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKS
                     ISRFARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQ
                     NVKLGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGI
                     GRALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVP
                     QYYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMIK
                     TLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQIL
                     LVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKSGIE
                     MEI"
                     /name="recombinase family protein"
     gene            1495..1719
                     /locus_tag="IQN08_15700"
     CDS             1495..1719
                     /locus_tag="IQN08_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="UOC45544.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDE
                     LKAIADVLGVKFVQAFILPDGEEIKISNE"
                     /name="helix-turn-helix transcriptional regulator"
     gene            1734..2603
                     /locus_tag="IQN08_15705"
     CDS             1734..2603
                     /locus_tag="IQN08_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing protein"
                     /protein_id="UOC45545.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYY
                     NSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIKRV
                     EQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIYPTA
                     LKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYCINEK
                     KAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEE
                     SSDERKQI"
                     /name="nucleotidyltransferase domain-containing protein"
     gene            2584..3318
                     /locus_tag="IQN08_15710"
     CDS             2584..3318
                     /locus_tag="IQN08_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="UOC45546.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGKR
                     VLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDFPE
                     ESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNEKGE
                     ILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPENMMD
                     IPGMADEMRRPMMLIVSAKKKM"
                     /name="class I SAM-dependent methyltransferase"
     gene            3351..4259
                     /locus_tag="IQN08_15715"
     CDS             3351..4259
                     /locus_tag="IQN08_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="UOC45547.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYDI
                     TYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLTLL
                     PLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNVTPY
                     VIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYISEDLW
                     EKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRDMYKKY
                     TGKTGCLDSTYAADIEERREQ"
                     /name="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
     gene            4256..4798
                     /gene="sat4"
                     /locus_tag="IQN08_15720"
     ARG             4257..4795
                     /name="sat4"
                     /gene="sat4"
                     /gene_name="streptothricin N-acetyltransferase Sat4"
                     /tool="amrfinderplus"
                     /drug_class="STREPTOTHRICIN"
                     /identity="100.0"
                     /coverage="100.0"
     gene            4891..5685
                     /gene="aph(3')-IIIa"
                     /locus_tag="IQN08_15725"
     ARG             4892..5682
                     /name="aph(3')-IIIa"
                     /gene="aph(3')-IIIa"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            5961..6533
                     /locus_tag="IQN08_15730"
     CDS             5961..6533
                     /locus_tag="IQN08_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="UOC45550.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLEE
                     AGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAMYK
                     IKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPASGG
                     QEPESRMIEPISLGFYEQNWYLIGFAG"
                     /name="HTH domain-containing protein"
     gene            6831..7727
                     /locus_tag="IQN08_15735"
     CDS             6831..7727
                     /locus_tag="IQN08_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="UOC45551.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYFK
                     GGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKNGN
                     LASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIIIDTV
                     PTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFVPYLV
                     DTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVLSMYEN
                     VFFEMIERIIQLENEKE"
                     /name="ParA family protein"
     gene            7826..8035
                     /locus_tag="IQN08_15740"
     CDS             7826..8035
                     /locus_tag="IQN08_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="UOC45552.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAKN
                     GGNVKEVMDQALEEYIRKYLPDKL"
                     /name="peptide-binding protein"
     gene            8052..8324
                     /locus_tag="IQN08_15745"
     CDS             8052..8324
                     /locus_tag="IQN08_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="UOC45553.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVN
                     LLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
                     /name="antitoxin"
     gene            8326..9189
                     /locus_tag="IQN08_15750"
     CDS             8326..9189
                     /locus_tag="IQN08_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="UOC45554.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSG
                     KTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEALIS
                     RLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYETMY
                     ADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISP
                     KETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTPPIPKT
                     PKLPGI"
                     /name="zeta toxin family protein"
     gene            complement(9452..10189)
                     /gene="erm(B)"
                     /locus_tag="IQN08_15755"
     ARG             complement(9456..10189)
                     /name="erm(B)"
                     /gene="erm(B)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(10314..10397)
                     /locus_tag="IQN08_15760"
     CDS             complement(10314..10397)
                     /locus_tag="IQN08_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="UOC45556.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
                     /name="23S rRNA methyltransferase attenuation leader
                     peptide"
     gene            10544..11224
                     /locus_tag="IQN08_15765"
     CDS             10544..11224
                     /locus_tag="IQN08_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="UOC45533.1"
                     /translation="MNPFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            complement(11247..11492)
                     /locus_tag="IQN08_15770"
     CDS             complement(11247..11492)
                     /locus_tag="IQN08_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010579578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="UOC45534.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVDP
                     PVLKTIRDLSYAKDIPMYEIVKLAMDGFCCKVLNKE"
                     /name="type III secretion system protein PrgO"
     gene            complement(11464..12417)
                     /locus_tag="IQN08_15775"
     CDS             complement(11464..12417)
                     /locus_tag="IQN08_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="UOC45535.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLFE
                     KDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGSPG
                     SEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAADYII
                     AMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQKTID
                     KYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYYEQHGD
                     IEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
                     /name="ParA family protein"
     gene            13029..14522
                     /locus_tag="IQN08_15780"
     CDS             13029..14522
                     /locus_tag="IQN08_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="UOC45536.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSKQ
                     LMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINTFV
                     VDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVIDVA
                     KKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSMKLES
                     DQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTLSLAYY
                     SSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELCQQWVSP
                     TLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLNLSKKELA
                     SQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLSKEKRLDYS
                     TRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
                     /name="primase C-terminal domain-containing protein"
     gene            14657..14953
                     /locus_tag="IQN08_15785"
     CDS             14657..14953
                     /locus_tag="IQN08_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="UOC45537.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTRE
                     KTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
                     /name="type III secretion system protein PrgN"
     gene            15054..15905
                     /locus_tag="IQN08_15790"
     CDS             15054..15905
                     /locus_tag="IQN08_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOC45538.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILDL
                     TVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAGKL
                     FKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILFFVG
                     YVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKGIRSI
                     LKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDLELPMK
                     DK"
                     /name="hypothetical protein"
     gene            15926..16209
                     /locus_tag="IQN08_15795"
                     /pseudo=""
     CDS             15926..16209
                     /locus_tag="IQN08_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            16571..17251
                     /locus_tag="IQN08_15800"
     CDS             16571..17251
                     /locus_tag="IQN08_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="UOC45539.1"
                     /translation="MNPFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            17409..17579
                     /locus_tag="IQN08_15805"
     CDS             17409..17579
                     /locus_tag="IQN08_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOC45540.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKVD
                     IADWLLQPREF"
                     /name="hypothetical protein"
     gene            17593..18195
                     /locus_tag="IQN08_15810"
     CDS             17593..18195
                     /locus_tag="IQN08_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="UOC45541.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQLQ
                     AMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENLRR
                     LINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAFDLF
                     LNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
                     /name="recombinase family protein"
     gene            18211..18888
                     /locus_tag="IQN08_15815"
                     /pseudo=""
     CDS             18211..18888
                     /locus_tag="IQN08_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /name="DNA topoisomerase"
     gene            19400..19696
                     /locus_tag="IQN08_15820"
     CDS             19400..19696
                     /locus_tag="IQN08_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="UOC45557.1"
                     /translation="MSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTNSASGVLRANE
                     IKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLKEKVVR"
                     /name="DNA recombinase"
     gene            19697..20113
                     /locus_tag="IQN08_15825"
     CDS             19697..20113
                     /locus_tag="IQN08_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="UOC45542.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQA
                     GIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFTSP
                     PPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
                     /name="recombinase"
     ARG             3352..4256
                     /name="ant(6)-Ia"
                     /gene="ant(6)-Ia"
                     /gene_name="aminoglycoside nucleotidyltransferase
                     ANT(6)-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(9453..10189)
                     /name="ErmB"
                     /gene="ErmB"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="98.37"
                     /coverage="98.79"
     ARG             4257..4798
                     /name="SAT-4"
                     /gene="SAT-4"
                     /gene_name="streptothricin acetyltransferase (SAT)"
                     /tool="rgi"
                     /drug_class="nucleoside antibiotic"
                     /identity="99.44"
                     /coverage="100.0"
     ARG             3352..4259
                     /name="aad(6)"
                     /gene="aad(6)"
                     /gene_name="ANT(6)"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="109.42"
     Typing          13030..14522
                     /name="rep_cluster_185"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.866"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgatggtat ctctggtacg aacactaaaa agcgtgacga atttaatcgc atgattgcag
       61 agtgtatgga cggtaacatc gacatggtta ttacaaaatc catcagccga tttgcgcgta
      121 acaccctaga ctgccttcaa tacattagac agctcaagga taagaacata tcagtttatt
      181 ttgagaaaga gaacatcaac accacggatg ccaaaggtga ggttttgctg actattatgg
      241 catctttggc acagcaggaa agccagagcc tttcacaaaa cgttaagctt gggctacagt
      301 accgatacca acaaggaaag gtgcaggtca accataagcg atttatgggc tacacgaaag
      361 atgaagatgg aaatttaact attgtccccg aagaagctga gattatcaaa cgcatctacc
      421 gagaatacct tgagggtcag agtctagtgg gcattggtcg agcccttgaa aaggatggta
      481 ttttaacagc agcgggaaaa ccaagatggc gaccagaatc agttaagaag atacttcaaa
      541 acgaaaaata catcggagat gcccttctgc aaaagactgt cacagtagat tttctgacca
      601 agaaacgagt gaaaaatgaa gggcatgttc cccaatatta tgttgaaaat agccatgaag
      661 cgattattcc taaagagtta tttttgcagg cgcaagaaga aattcatcgg agaagcaata
      721 tctacacagg agaaggcaag aacaaaagaa tttacagtag caagtacgct ttaagtgcca
      781 tcaccttctg tggagattgt ggcgatattt atagacgggt ctattggaat atccacggaa
      841 gaaaagaatt tgtctggcga tgcgtgacta gaatcgagca aggtcctgaa gtttgtaaga
      901 accgaaccgt aaaagaagat gaactttatg gtgcagtaat gactgcgatt aataagctac
      961 ttgcaggtgg gaacaatatg ataaagacac tggaagagaa tattcatgct gtgattggcg
     1021 aaaccacaga ataccaaatt tcagagatta acaactcact agaggaaaaa caaaaagaac
     1081 taatcaagct ggcgaacaag ggtcaagact atgaccactt agcagatgag attgatgagc
     1141 tgagagacaa gcgacagatc cttttagtag aagatgcctc cctcagtggc gagaacgagc
     1201 gaatcaatga gctgattgaa tttatccgca agaacaaatt ccgcacctta gaatatgatg
     1261 ataagcttgt aaggaagata atccagagcg ttacagtcta tgaaggccac ttcgtcatag
     1321 ccttcaaatc tggcatcgaa atggaaatat gaaaaccaga agtaaatagc ccatgactct
     1381 acagtagagt tgtgggtttt cttttgcttg ctgattataa taatatttta ataactattg
     1441 tgtttatcaa ccaaatggtt tataatattc ttagcgtaaa cttggaggtg ctgtatgact
     1501 acggcagaaa tgattaaaga actgtgtgag caaatgaata taagtgtttc cgaacttgct
     1561 agacgtattg gccagactcc acagaatttc aataaaaaat tacaacgaga aacagtaacc
     1621 ttggatgagt tgaaggccat tgctgatgtg ctaggtgtca agtttgtgca ggcatttatt
     1681 ttaccagatg gagaagaaat aaaaatatct aacgaataaa ggaggatgtt tatatggtag
     1741 gtaatgttat tcaaatagta acagagaaat tatcatcttt gccttttata gaaggcatcg
     1801 tattaggggg ctcacgtgca agaggcaccc atacagaaaa ttctgatata gatatcggaa
     1861 tctattacaa ttcagactca tttgacctga cagcgattaa tcaaattgct acagagttgg
     1921 atgatgagaa tagaaacaac cttgttgtac ctcccggagc gtggggtgat tgggttaatg
     1981 gcggtggatg gttagttata aacgggtgtc atgttgactt gatattacgt gatataaaac
     2041 gggtggaaca aataatcaaa gatacggagc aaggaattgt tactgccaat tatcagaccg
     2101 ggcatcccca tggttatata agtgctatgt atcgtggaga attagcgatt agtaaaatac
     2161 tatatgctaa gaacgaaagc ttatgcgaat taaaaaagca ggcagaaatt taccctactg
     2221 ctctaaagaa aagcttgatg aactttttta tatttgaagc tgaattctct ttaatgtttg
     2281 taaaagcaaa tgcgggggca gaggataaat attatattgc aggccatgtt tttcgtatta
     2341 tttcatgctt aaatcaagta ctatttgcat gtaataatgc ttattgcatt aacgaaaaga
     2401 aagctataaa actgcttgaa acttttgaat ataaacctga aaaatatgcc gaaagagtta
     2461 atcatatttt tgaagtactc ggtctttctc tttttgaatg ctacgacatg accgagaaac
     2521 tttataaaga agtgaatgaa attgtatcgg agataaataa ctttttaaac gaggagagtt
     2581 cagatgaaag aaaacaaata tgatgataat atattttttc aaaaatacag tcaaatgagt
     2641 cgctcgcaga aaggactggc tggtgcggga gaatgggaga ctttgaaaaa gatgctacct
     2701 gattttaagg gtaagcgtgt gcttgattta ggatgcggct atggatggca ctgtatatat
     2761 gcgatggaaa acggtgcttc ctctgtagta ggtgttgata tttctcataa aatgctcgaa
     2821 gtagcaaaag gaaaaaccca ttttccacag attgaatatg aatgctgtgc catagaagat
     2881 gtggatttcc cagaggagag ctttgatgta atactaagtt cgcttgcgtt tcattatgta
     2941 gcagactatg agaatttaat aaaaaagata tataggatgc tgaaggctgg tggcaattta
     3001 gtttttacag ttgaacatcc tgtttttact gctcatggaa cacaagactg gtattataac
     3061 gaaaaaggag aaatactgca tttcccggtg gacaattatt attatgaggg caaacggaca
     3121 gctatgtttt tggaagaaaa ggttacaaaa tatcatagaa cactgaccac atatctaaat
     3181 acactgcttt caaatagttt tataataaat cagattgtgg agccacagcc gccagagaac
     3241 atgatggata ttccggggat ggcggatgaa atgcgacgcc caatgatgct gattgtatcg
     3301 gcaaaaaaga agatgtaata atatagaaaa aataaacgag gagtatgtaa atgagatcag
     3361 aaaaagaaat gatggattta gtactttctt tagcagaaca ggatgaacgt attcgaattg
     3421 tgacccttga ggggtcacgc gcaaatatta atatacctaa agatgaattt caggattatg
     3481 atattacata ttttgtaagt gatatagaac cgtttatatc taatgatgac tggcttaatc
     3541 aatttgggaa tataataatg atgcaaaagc cggaggatat ggaattattc ccacctgaag
     3601 aaaagggatt ttcctatctt atgctatttg atgattacaa taaaattgat cttaccttat
     3661 tgcccttgga agagttagat aattacctaa agggcgataa attaataaag gttctaattg
     3721 ataaagattg tagaattaaa agggacatag ttccgactga tatagattat catgtaagaa
     3781 agccaagcgc aagggagtat gatgattgct gcaatgaatt ttggaatgta acaccttatg
     3841 ttattaaagg attgtgccgt aaggaaattt tatttgctat tgatcatttt aatcagattg
     3901 ttcgccatga gctgctgaga atgatatcat ggaaggtcgg catcgaaaca ggctttaaat
     3961 taagtgtagg caagaactat aagtttattg aaaggtatat atccgaggat ttgtgggaga
     4021 aacttttgtc cacctaccgg atggattcct atgaaaacat atgggaagca ttatttctat
     4081 gccatcaatt gttcagggcg gtatccggtg aggtggcgga aaggcttcat tatgcctatc
     4141 cggagtatga taggaatata acaaaatata ccagggacat gtataaaaaa tacactggta
     4201 aaaccggctg cctggatagc acatatgccg ctgatataga agagaggcgg gaacagtgat
     4261 tacagaaatg aaagcagggc acctgaaaga tatcgataaa cccagcgaac catttgaggt
     4321 gataggtaag attataccga ggtatgaaaa cgagaattgg acctttacag aattactcta
     4381 tgaagcgcca tatttaaaaa gctaccaaga cgaagaggat gaagaggatg aggaggcaga
     4441 ttgccttgaa tatattgaca atactgataa gataatatat ctttactacc aagacgataa
     4501 atgcgtcgga aaagttaaac tgcgaaaaaa ttggaaccgg tacgcttata tagaagatat
     4561 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
     4621 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
     4681 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
     4741 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
     4801 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
     4861 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
     4921 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
     4981 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
     5041 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
     5101 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
     5161 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
     5221 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
     5281 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
     5341 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
     5401 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
     5461 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     5521 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     5581 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     5641 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     5701 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     5761 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata gagttctata
     5821 ttagagatgt aaagagtttt ggtgaagagg tttgtgagta tggcttttat aaccagggtg
     5881 caatgagaag cactaacagc agctagctta agaacgcata ggatatactt tttatggaag
     5941 tcacagagga ggaataaaat ttgaacagga ttaacagagt aacctctatt cttattcagc
     6001 tgcaatcaaa aaaaataatt cctgccaaag aaattgcaca gcgatttaat ataagcttaa
     6061 ggacagttta cagagatatc cggacacttg aagaagccgg aataccaatt ggatctgagg
     6121 ccggaaaagg atattttctt gtagagggct tcctacttcc gccggtaatg tttacagcgg
     6181 cggaagtggg tgcattgatt acagcaggga aatttttaaa ttgccatgga gatgaatcat
     6241 ttataaagga ttttgattca gctatgtata aaatcaaatc tattttgaag catggtgaaa
     6301 aaaactatgc acaggagttg gagaacagta ttaacgtgta cagcacatct ggacagaaaa
     6361 atacattggc ggataacgtt attgcagcaa ttcagactgc aatctgcaat aaaagggtaa
     6421 tatcaattca atatcctgca tcaggagggc aagaaccgga aagcagaatg atagagccta
     6481 tatcactggg gttttatgaa cagaactggt acttaatcgg ttttgcaggc taagaaatga
     6541 atataggaat tttcgtgtgg atcggattaa aagcatttgc atagaagaag agctctgtca
     6601 aagccatgat ggctctttgg aacaaatatt aaaacaaatg ctttcatata aaaaactata
     6661 tgccctacaa tgccgttgta aaaattgggg aaaagggata tattgatttt atatctttct
     6721 caaaataaat ataaaagccc tttaaagagg gcttttatat attaatcaca aatcacttat
     6781 cacaaatcac aagtgattaa tcacaaatca cttgtgattt gtgattctta atgatacaat
     6841 attactatac aaaaaaagaa tggggcgtag ttatggagaa ggaaaaacta aaaatacttg
     6901 aagaattaag acgtatttta aacaataaaa atgaagcaat tattatcttg aataattact
     6961 ttaaaggtgg tgttggaaag tccaaattat cgactatgtt tgcttacttg acagacaaat
     7021 tgaatttaaa agttttaatg atcgataagg acttacaggc aacattgaca aaagacttag
     7081 caaaaacttt tgaggtagaa ttgccacgtg tcaattttta tgaaggcttg aaaaatggaa
     7141 acttggcttc ttctattatt catttgactg ataatttaga cttgatccct ggcacgtttg
     7201 atttgatgtt actgccaaaa ttaactcgct catggacgtt tgaaaatgaa agtagattgc
     7261 ttgctactct tttagcacct ttaaaaagtg actatgatct tattattatt gatactgtac
     7321 caacgccaag cgtttataca aataatgcaa tcgtggcgag tgattacgtt atgatccctt
     7381 tacaagcaga agaagaaagt acaaacaaca ttcaaaacta tatttcctat ttgattgatt
     7441 tgcaagaaca gtttaaccct ggactagata tgattggttt tgttccttat ttagttgata
     7501 cggacagcgc aacgataaaa tcaaacctgg aagaactgta caaagaacat aaagaggata
     7561 atttggtttt ccgaaatatt atcaagcgaa gtaataaagt aagtacttgg tctaaaaatg
     7621 gcattacaga acacaaaggc tatgacaaaa aagttttgtc tatgtatgag aacgtatttt
     7681 ttgaaatgat tgagcgaatc attcaattag aaaatgaaaa agaatagaat cacaaatcac
     7741 aagtgattaa tcacaaatca cttgtgattt gtgataggtg atgataaaat aaatagtaag
     7801 aagaaataga aagaagtgag cgatcgtggg aaatttaggc gcacagaaag caaaacgaaa
     7861 tgatacgcca atcagcgcaa aaaaagatat aatgggggat aagacggttc gtgttcgtgc
     7921 cgacttgcac catatcataa aaatcgaaac agcaaagaat ggcggaaacg taaaagaagt
     7981 tatggatcaa gccttagaag aatatatacg gaaatattta cctgacaaac tttaaaagga
     8041 gtgaaaatga aatggcagtt acgtatgaaa aaacatttga aattgaaatc attaatgagt
     8101 tatcggcaag tgtttataat cgggtattaa attatgtttt gaatcatgaa ctagatacta
     8161 aaaatactcg tttactagaa gtgaatcttt taaatcaatt agaagtggca caagaagttg
     8221 atttatttca acaaccattt gaagaattac aagctattca tgagtattgg cggtcaatga
     8281 atcaatattc aaaacaaatt ttgaataaag agaaagtggc ttaacatggc gaatattact
     8341 gacttcaccg aaaagcaatt tgaagatcgt ttagaaaaga atgttgaacg actaactaaa
     8401 aatagactag cggttgaatc gccaaccgct tttttacttg gtgggcaacc agggtcaggg
     8461 aaaaccagtt tgcgatcagc aatttttgaa gaaacacaag ggaatgttat tgtcattgat
     8521 aatgatacct ttaaacaaca gcacccaaat tttgacgaat tagtgaaact ctatgaaaaa
     8581 gacgtagtaa aacacgttac cccttattcg aatcgcatga cagaagcact tattagccgt
     8641 ttaagcgatc aaggctataa tttagtgatt gaaggaacag gacgaacaac agacgttccc
     8701 attcaaacag ccacaatgct tcaatctaaa ggttatgaaa caaaaatata cgtcatggca
     8761 gtaccgaaaa ttgaatcata cttaggaacg attgaacgat atgaaaccat gtatgcagat
     8821 gatccaatga cagctagggc aacaccaaaa caagcgcatg atattgttgt caaaaactta
     8881 ccgaccaatt tagaaaccct tcataaaacg ggcttattta gcgatataag gctttacaat
     8941 agagaaggag tgaaactcta ttcaagctta gaaacgcctt ccattagtcc gaaagagacc
     9001 ttagaaagag aattaaatcg taaagtatca gggaaagaaa ttcaaccgac tttggagaga
     9061 atagagcaaa aaatggttca aaatcaacac caagaaaccc ctgaatttaa agcaattcaa
     9121 caaaaaatgg aaagcttgca gctacctaca ccaccaatac ccaaaacacc taaacttcca
     9181 ggtatttaaa ccttaaaaaa ggaaaagagt agttaccaaa aaacggtaac tactcttttt
     9241 ttataaaaac atttcctcat gttggggtcc cgagcgccta cgaggaattt gtatcgataa
     9301 gaaatagatt taaaaatttc gctgtttttt tctaatttca ttttgaggtg aattaattgg
     9361 tagttattag aggtgcccta tacctaataa tttatctaca ttccctttag taacgtgtaa
     9421 ctttccaaat ttacaaaagc gactcataga attatttcct cccgttaaat aatagataac
     9481 tattaaaaat agacaatact tgctcataag taacggtact taaattgttt actttggcgt
     9541 gtttcattgc ttgatgaaac tgatttttag taaacagttg acgatattct cgattgaccc
     9601 attttgaaac aaagtacgta tatagcttcc aatatttatc tggaacatct gtggtatggc
     9661 gggtaagttt tattaagaca ctgtttactt ttggtttagg atgaaagcat tccgctggca
     9721 gcttaagcaa ttgctgaatc gagacttgag tgtgcaagag caaccctagt gttcggtgaa
     9781 tatccaaggt acgcttgtag aatccttctt caacaatcag atagatgtca gacgcatggc
     9841 tttcaaaaac cactttttta ataatttgtg tgcttaaatg gtaaggaata ttcccaacaa
     9901 ttttatacct ctgtttgtta gggaattgaa actgtagaat atcttggtga attaaagtga
     9961 cacgagtgtt cagttttaat ttttctgacg ataagttgaa tagatgactg tctaattcaa
    10021 tagacgttac ctgtttactt attttagcca gtttcgtcgt taaatgccct ttacctgttc
    10081 caatttcgta aacggtatcg gtttctttta aattcaattg ttttattatt tggttgagta
    10141 ctttttcact cgttaaaaag ttttgagaat attttatatt tttgttcatg taatcactcc
    10201 tgaagtgatt acatctataa ataaatacag aagttaaacg atttgtttgt aattttagtt
    10261 atctgtttaa aaagtcataa gattagtcac tggtaggaat taatctaacg tatttattta
    10321 tctgcgtaat cactgttttt agtctgtttc aaaacggtag atgttttatc tacattacgc
    10381 atttggaata ccaacatgac gaatccctcc ttcttaatta caaattttta gcatctaatt
    10441 taacttcaat tcctattata caaaattttg gttctgttgc aaagttttaa atctactatc
    10501 aaataaggta gaataataga aaaagatagc aggaggaatg acgatgaatc cttttaaagg
    10561 aaagcaattt cagcaggatg tgattattgt agccgtgggc tactatcttc gttataacct
    10621 tagctatcgt gaagttcaag aaatcttata tgatcgtggc attaacgttt ctcatacgac
    10681 gatttatcgt tgggtgcaag aatatggcaa actactctat caaatttgga aaaagaaaaa
    10741 taaaaaatcc ttttattcat ggaaaatgga tgaaacgtac atcaaaatta aaggaaaatg
    10801 gcattatttg tatcgagcca tcgatgcaga tggtttaacc ttggatattt ggttacgtaa
    10861 aaaacgggac acacaagcag cctatgcttt tcttaagcgg ttagtgaagc agtttgatga
    10921 accgaaggtt gtagtcacag ataaagcccc ctctattaca agtgccttta agaaactaaa
    10981 agaatacggc ttttatcaag ggacagaaca tcgtaccatt aaatacctga ataatttgat
    11041 tgaacaagac catcgtccag taaagagacg caataaattc tatcgaagtt tacgcactgc
    11101 ctctaccacg attaaaggca tggaagccat tcgaggatta tataagaaaa cccgaaaaga
    11161 aggcactctc ttcgggtttt cggtctgtac tgaaatcaag gtattattgg gaatcccagc
    11221 ttaaatcata gataccgtaa gggattttat tctttattta aaactttgca acagaacccg
    11281 tccattgcaa gtttgactat ttcatacata ggaatatctt ttgcataact aagatcccga
    11341 atggttttta gtacgggagg atctacttga actggttttc tttctttcgc agtgtacttt
    11401 cgttccccta actcgacatt aatatcatct ttaattgaaa tatcttcagc accaattttc
    11461 ttcttatctt tgttcgttgg taataatgcc atttagttta atctcctttc caagaggtag
    11521 gatatgattg gccattgagt cagcatattt aggtgtataa tcaaatcctt caatatcacc
    11581 atgttgttca tagtattcaa ttctttcttc taattctgta aagatgtctg caaaaatagc
    11641 ccaccatctt cgatctacat aatcatttaa ttggatacca ttctcaccat acatttctaa
    11701 ccgatccgag cctttaacaa tgcttttaaa aatgtttgat tcaccatact tatcaattgt
    11761 tttttgatag tttgcgtgtt gagaggaacg tcgcactgag aaaagaacag gcaaaattcc
    11821 aatgatttga agatgagact cttcaaaatt cgtgacaatc ggaactaaaa ctttattaat
    11881 aaaatcttct gttccttcca ttgcgtaccg ttttaattct tgcattgcaa taatataatc
    11941 tgcagcagtt aagaatgcac gaaccacatt atctgttgat ggtgggcagt cgataaagat
    12001 ataatcgaaa ttttttctca attcttctaa aggtttaatg aaggccaatg atctcttacg
    12061 acgatcagga tatttttcgg acaagtattc attaagttct tcagatccag gagagccagc
    12121 cataatatat aaatgtggac ttacttgagt aatacctgaa acccagtccc cttttttgat
    12181 tgctgcagac atggatcgat caaagctagt aatttgatac gttgatccaa tgttggaagt
    12241 agtatttctt tgttggtcat aatcaattag caacacgtct ttttcaaaaa gttgactaga
    12301 aacaatcgct aataaatcat tgtctgtagt cttaccaatg ccgccttttc tacctgtaag
    12361 cattacaact tttcctagtt tatctgatgt tttaaatgca gtccgtaagt tttccatagt
    12421 tccctccaat ttcctatact aaatatattt agtatacttc atatacttaa tatattcgat
    12481 atatctaata tactccaaat tgataagaat tcagacttta taaggaatat agtggatata
    12541 ctaaatatat ttgctatatt cagtatatca gatatattca aaatgtcaaa atatatttgt
    12601 gcgttttgcg ttgacaattt agagtgaaaa gaggtaaact taattctata gaaaccacag
    12661 ataattcact atttacaaag tagcaaaagc agtggtactc tttaaacaga aaaattacat
    12721 aaaaaaaaga gacggaaata attgatttta gcagaatcaa ttatttctga atcacatggt
    12781 taggacatgt gttcacgctc gtctcaagaa tatgtaagta gctaccaact acttatatta
    12841 aaagttgatc ggataccttt tccgattgcc tttattctac caaaaaaagg cgagcaactc
    12901 aacagaagaa cgtttattta gtgtacttaa attaaatatt cttaaacact gaagagagaa
    12961 cacatactat ggctgagatt aaagctaaac tgtgttctct ttttgtttga gaaaggacgt
    13021 tacaatcgat gttgagcgaa atttattcag ctattcttaa aaacggctta cgtacataca
    13081 aatatcgaaa cagcaccatg aaacctgcag gtcatattga ttccagtagg agtggtgcta
    13141 tctttgcgta ccgtagcaag caattaatga atgttgggcg tggtatggtc attacttcgg
    13201 aagaagctat tttagagaac gaaaagaaac taacgcattg gacacctaat acctatcgct
    13261 ttggtactta tgcggatgat tatcgaacag tggttaaagg tcattctgag aaaaacttaa
    13321 gtcaaatcaa cacatttgta gttgatatcg attcaaaaga gaaccatcaa ggcgaaatta
    13381 ttttagcctg tttagatcaa gtgggctaca tgccaacgct aattttagaa tcagatcatg
    13441 gttatcaagt ttattttgta ctaaagacac caggctatgt cacaaagaaa agcaatttca
    13501 aggtgatcga tgtagcaaaa aaaatttcaa aaacaattcg tctacagttg gctgaaaaat
    13561 tgtccggagt ggatctagga tgtaaccact tcggaattgc tcgattccca caagaaaata
    13621 atattgtttt taaagaaatg aactatcagt attcttttgc tgactggatt caatggtcga
    13681 tgaagcttga atcagatcaa aaaacaaaag aagatcgaga acgaaaacta attgtctttc
    13741 ctgagaaaaa agaaattcgt caagtagatg aaccgtggtt cgatatgtta cttcataaag
    13801 caaacatcgt cggcggagaa ggacgtcttg gtcgaaataa tgtgattttc acgctttctt
    13861 tagcttatta ctcttcaggt tatgcattgg atacttgcga atacaatatg ttcacattca
    13921 acgaacggtt acaggaaccg ctatctgaag cagaattagt aaagattgta cgtagtgctt
    13981 attctggtaa ttatcaagca gcaagcagag aattcattat tgaattgtgc cagcaatggg
    14041 tttcaccaac cttaaaaaat aatgatctgt tcattcaacg acgtggctgg tggaaattta
    14101 aaaagccacg agatcaaaga aaatatagtc ataaaaatga atggaaagaa gatctattgg
    14161 cttatctaag cgaaaaaagt cttgctagtg agccttattt gaacctgtca aaaaaagaat
    14221 tggcttcaca actaacgatg cccaagcgaa cacttgataa gatcttaaat gagttacgag
    14281 aggagaataa agtcttctac aaggtaacac gaggaagaaa tggcggaatg atcattgctt
    14341 cagtcaaaac gttgttcgcg agggttattc aactaagtaa agaaaagaga ctagattatt
    14401 caacaaggat cacagaggta tttggactca acgagagctt agtagaagag acatttagac
    14461 aattatcaac ccccttgaac cctcttaaag aagtgaatct ttttgaagta gatacaggtt
    14521 gaaaaataaa aggtgcaaat tgccttatac aatatatcta tgatacttct gtaggtgttg
    14581 ctccctttgc ctagctacgt gtaggtggta tacttataac tgcgtatgca gtcttatgta
    14641 tcatggagga agttgtatgt cgttaaaaaa ttatgtctat tcacatcctg ttaatgtgtt
    14701 tatcattggt cgtttgggat tatctgtgga agtgttctgt gagctttatg gttttaagca
    14761 ggggactttg tcttcttggg ttaccaggga aaaaacagtt gcttctttgc cgattgagtt
    14821 tcttcatggg ttgtccttag cttctgggga gactatggat caggtatatg attgtttgtg
    14881 tgtgttagag caggagtata ttgagtataa aatagctaac gagttaagaa aaaggaagaa
    14941 gtatatccag tagtagtttt tatttactta aaaaattggt tcattgagca acctatcgct
    15001 gctaaagtag caaaggaaaa cgttaaagga aagaatttag ggaagaaatc gaaatgaaat
    15061 cggacgactg gattgaacac acctggcttc ctctgaaaag aaatagcaaa cgaaagaaat
    15121 tttgggtatt gcaattatgg ccattaggaa aactttgtag ttacggggca cgacttattc
    15181 ttgatttaac cgtttactcg attggagtat ttggaacggc ttattacttg ataaatgatc
    15241 ccaataggat tgcagcatca gacattttta cagtgctaat gacgttgatt tttgtcatgc
    15301 tattgtctat ttttactcaa ttagtcatgc aatttaccag gcaaaaatta acagcaggaa
    15361 aacttttcaa aagcctattc gatttaattg ttgtaaatgc aattctagcg actttaataa
    15421 aacaaattga acaatctgga agaacaacaa aaggagtgaa ggatttttta ttgacgttta
    15481 ttcactttgt aaatggacac atgtatttgt ttgaagtggg tattattctc ttttttgtcg
    15541 ggtatgtatc cacgaaactt attaaacgtt caattgaaaa agaaattgaa attgacctga
    15601 ttccgacact tcttggttat gagtacaatg ttgattttgg aagatatgac gaattgatca
    15661 atacggatat cgttcacacg agtatccatg aatctactgt taagggcatc agatctattt
    15721 taaaaaatgg ccaggaaatg aaagcaatag atcgaagaaa agagatctac aatttttatg
    15781 aagtcgaagc aagcctacga tatggcatcg gaaaattgtg gttagatgga agtaatattc
    15841 gtcaaaacaa accatacatt ttttggaaca gtagtgattt agaacttcca atgaaagata
    15901 aataattatg gagaatcgtt tgaaaatgat tcctctatac aggattgtgc ttatagttgg
    15961 catggagtta aaaaccttaa agaaggagca gttattcaaa gtgaaagaac gtcataatca
    16021 gttactatgt agtaaaacgg gtacgatgaa cattgaatac cgaacagaat atttagaatt
    16081 aacgaatcaa actgattttt tgttgattcg aaagcaacaa gagcaagaaa aaaattaatg
    16141 gctacttata gccaattgat tgatgaatgg gaacaattca aggaatctaa aattccagaa
    16201 cgtctctaaa agcctttatt tgtaaagagc ataaggaaac gtgggaacac tttttaaaaa
    16261 aagtgttcca ataggctgtg cctattacgt ttctcaaaaa taaatgtggg aacaaaatat
    16321 ttaaaaatag gatagtgaaa ggatagtgaa ggatagtaaa aggagggtag taggatagca
    16381 aaaaggatag gctccttttc gctatccttt ttaaattgga ggaattaata tggaagtaag
    16441 aataagaaat gtggatgaga aaatcgtaag agaaattgat cgaagatgtg acgaacggtt
    16501 ctgttgcaaa gttttaaatc tactatcaaa taaggtagaa taatagaaaa agatagcagg
    16561 aggaatgacg atgaatcctt ttaaaggaaa gcaatttcag caggatgtga ttattgtagc
    16621 cgtgggctac tatcttcgtt ataaccttag ctatcgtgaa gttcaagaaa tcttatatga
    16681 tcgtggcatt aacgtttctc atacgacgat ttatcgttgg gtgcaagaat atggcaaact
    16741 actctatcaa atttggaaaa agaaaaataa aaaatccttt tattcatgga aaatggatga
    16801 aacgtacatc aaaattaaag gaaaatggca ttatttgtat cgagccatcg atgcagatgg
    16861 tttaaccttg gatatttggt tacgtaaaaa acgggacaca caagcagcct atgcttttct
    16921 taagcggtta gtgaagcagt ttgatgaacc gaaggttgta gtcacagata aagccccctc
    16981 tattacaagt gcctttaaga aactaaaaga atacggcttt tatcaaggaa cagaacatcg
    17041 taccattaaa tacctgaata atttgattga acaagaccat cgtccagtaa agagacgcaa
    17101 taaattctat cgaagtttac gcactgcctc taccacgatt aaaggcatgg aagccattcg
    17161 aggattatat aagaaaaccc gaaaagaagg cactctcttc gggttttcgg tctgtactga
    17221 aatcaaggta ttattgggaa tcccagctta aatcatagat accgtaaggg attttattct
    17281 ttatttaaaa ctttgcaaca gaacctttta aggtcaatag agtatactta tttgtcctaa
    17341 tatggtttta gcagtataat tgacttggtg aataggtcat ttaagttggg cataatagga
    17401 ggagtaaaat gaaaaaattt atttatcgag ttttagaaaa tgacgaagtg gtggctattt
    17461 ttaatgagca acaatatgcg caagatttta tcgcttacga aaagacaatt tctgataagc
    17521 aatttgaaat tgaaaaagta gatatagctg attggttatt gcaaccgaga gaattttaga
    17581 ggttggttga aaatggctaa aattggttat gcacgtgtca gtagcaaaga acagaactta
    17641 gatagacaac tgaaagcgtt agagggcgtt tctaaggtct tttcagacaa attaagcggt
    17701 caatcggtcg aacgcccaca attacaagct atgcttaact atattcgtga aggggatata
    17761 gttgttgtta ctgaattaga tcgattagga cgaaataata aagaattaac agaattgatg
    17821 aatcaaattc aaattaaggg ggcaaccctg gaagtcttaa atttaccctc aatgaatggt
    17881 attgaagatg aaaatttaag acggctgatt aataatttag tgattgaatt gtataagtac
    17941 caagcggaat ctgaacgcaa acgaattaaa gaacgccaag cccaaggaat tgaaattgct
    18001 aagaaaaaag gaaaattcaa agggcgacaa cctaaattca aagaaaatga tccacgttta
    18061 caacacgctt tcgatttatt tttgaatggt tgttccgata aagaagttga agaacaaact
    18121 ggaattaatc gccgaacgtt tagaaggtat cgatcaagat acaacgtgac agtagatcaa
    18181 agaaaaaaac aatgaaaaga gggatagtta atgagtacgg ttattttagc tgaaaaacca
    18241 agccaggcat tagcctacgc aagtgcttta aaacaaagca ccaaaaaaga cggttatttt
    18301 gagatcaaag acccactatt tacagatgaa acgtttatca cctttggttt tgggcattta
    18361 gtcgaattag cagaaccagg tcattataac gaaaagtggc agaactggaa acttgaatct
    18421 ttgccgattt ttcctgatcg atacgatttt gaagttgcaa aagataaggg aaagcagttt
    18481 aaaattgttg ctgaacttct caaaaaggca aatacaatta ttgtcgcaac agatagcgac
    18541 agagaaggtg aaaatatcgc ctggtcgatt atccataaag caaatgcctt ttctaaagat
    18601 aaaacgtata aaagattatg gatcaatagc ttagaaaaag atgtgatccg aagcggtttt
    18661 caaaatttgc aaccaggaat gaattactat cccttttatc aagaagcgca aacacgccaa
    18721 attgccgatt ggttgatcgg catgaacgca agccctttat ataccttgaa tttacaacaa
    18781 aaaggcgtac aaggtacatt ttcattagga cgtgtacaaa caccgacttt atatctcatt
    18841 tatcaacgcc aggaagcaat agaaaacttt aaaaaagaac cttttttcct gaataattca
    18901 tagttttact aatagtcttt cctaacattg atttaatagt ctttaaagta ttctgcttca
    18961 tcacccatta ggtgatgaaa aaagaacccc tttctgattt agcggacaaa tactttgacc
    19021 taatctcaga agatataaaa gaaaaaataa taaaatatcc gatttatact ttgtataaag
    19081 aactaaagta gttagcaaat catttacagc aaatcactat gttccacata ccattttaca
    19141 tacttacctt atccacgaca catgttgaga tagtatcgaa gataaagaaa gttgctccat
    19201 gttgtttttg cgagatgggg aaatcaaagc cacattgaca accatgatga ataagcttgc
    19261 tttcagtcat aagctaatcc tagaaccact tttcaagtca gttagccaaa tcgatgaaga
    19321 aagcgaccgt gaaagaatgg acgctattga taagctaatg gagcaactat tggaagaacg
    19381 caataccctt attgcactga tgtccaaagg tttcctagaa cctgctcttt ttaatcagga
    19441 acgaaatgtt ttagatagtg agattaaaaa tcttactacc gaaaaaacaa acctggtaac
    19501 gaactctgcg agtggggttt tgcgagcaaa cgagataaag gacctcatta attatgtgtc
    19561 agcagataat tttaatggtg actacacgga agaactattt gaagaatttg tagtgaacat
    19621 cattgtaaat tccagggatg agctgacatt caatttgaaa tgcggtcttt ccctgaaaga
    19681 aaaggtggtg agataaatgg catatattcc atatggatac aaaattcaag atggagtggt
    19741 tactgtcgat gaaaaggcag caggtcaagt aaaggtattc tttgagaaat acatatcagg
    19801 actatccctt acagtggctg gcgaacaggc aggtattgaa aagacacatt ctgtgatggg
    19861 gcgcattttg aaaaacgtca actaccttgg aaatgatacg tatccagcaa ttattgataa
    19921 agagatattt gataaagctg aagaagttag agataaacgt gcaaaggatt taggacgagt
    19981 ggtagagctt gccgctttca cctctccccc tcccaaagaa cgattcaaaa tgaaaaaggc
    20041 agataataag atgccagtgg atccttttga acgagcagaa tacttataca gtctgataga
    20101 aagcgaggaa taaagtgaca gagaaaaata taatggttat tcctgctcgt aaaagagtag
    20161 gaagtacagc cgcaaaagaa aagataaaga aacttcgtgt tgctgcctat tgccgtgttt
    20221 ctacagaaac agaagaacaa aattctagct atgaggttca ggtctcacac tatacagagt
    20281 ttataaagaa aaataatgaa tgggagtttg ctggcatctt tgcag
//
