LOCUS       CP063607               19255 bp    DNA     circular BCT 04-APR-2022
DEFINITION  Enterococcus faecium strain BMT-2-3-8 plasmid pSS-3-8_03, complete
            sequence.
ACCESSION   CP063607
VERSION     CP063607.1
DBLINK      BioProject: PRJNA669626
            BioSample: SAMN16483156
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19255)
  AUTHORS   Both,A., Kruse,F., Mirwald,N., Franke,G., Christner,M., Huang,J.,
            Hansen,J.L., Kroger,N., Berneking,L., Lellek,H., Aepfelbacher,M. and
            Rohde,H.
  TITLE     Population dynamics in colonizing vancomycin-resistant Enterococcus
            faecium isolated from immunosuppressed patients
  JOURNAL   J Glob Antimicrob Resist 28, 267-273 (2022)
   PUBMED   35134550
REFERENCE   2  (bases 1 to 19255)
  AUTHORS   Both,A., Kruse,F., Christner,M., Huang,J., Mirwald,N., Franke,G. and
            Rohde,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) Institute of Medical Microbiology, Virology
            and Hygiene, University Medical Center Hamburg-Eppendorf,
            Martinistr. 52, Hamburg, Hamburg 20246, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Trycycler v. v0.3.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.5x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/27/2020 17:58:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.13
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,079
            Genes (coding)                    :: 2,968
            CDSs (with protein)               :: 2,968
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 40 of 111
            Pseudo Genes (incomplete)         :: 66 of 111
            Pseudo Genes (internal stop)      :: 13 of 111
            Pseudo Genes (multiple problems)  :: 7 of 111
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..19255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="BMT-2-3-8"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pSS-3-8_03"
                     /geo_loc_name="Germany: Hamburg"
                     /lat_lon="53.33 N 10.1 E"
                     /collection_date="2014-07-23"
                     /collected_by="Institute of Medical Microbiology, Virology
                     and Hygiene, University Medical Center Hamburg-Eppendorf"
     gene            complement(join(18992..19255,1..36))
                     /locus_tag="IQN08_15405"
     CDS             complement(join(18992..19255,1..36))
                     /locus_tag="IQN08_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="UOC45488.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFLV
                     CGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
                     /name="BRCT domain-containing protein"
     gene            complement(77..694)
                     /locus_tag="IQN08_15410"
     CDS             complement(77..694)
                     /locus_tag="IQN08_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="UOC45489.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFKR
                     PEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTASY
                     KQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPPEFP
                     AIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
                     /name="recombinase family protein"
     gene            complement(1019..1414)
                     /locus_tag="IQN08_15415"
     CDS             complement(1019..1414)
                     /locus_tag="IQN08_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOC45490.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIVD
                     YFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKKKE
                     YNYIVKAKVSKRFIFNPKLEIYSVVIE"
                     /name="hypothetical protein"
     gene            complement(1494..3845)
                     /locus_tag="IQN08_15420"
     CDS             complement(1494..3845)
                     /locus_tag="IQN08_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="UOC45491.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQV
                     ELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVYTP
                     YLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLNVNY
                     LEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGNSGNY
                     VRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKGLLSSA
                     GDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTTEELNNI
                     FSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAVDLDMLGE
                     EILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMSTGYSGNLG
                     FPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNYIAKYNQKVI
                     NQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRKIYDEYRNKLT
                     NSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWLGDLMTCWGEVK
                     KLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDSTALSALKTYYCDK
                     GAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFIDKIVNNSLGTDFT
                     YLDFIGDEGKVANGFSEKIIQFQENRSKLN"
                     /name="DUF1906 domain-containing protein"
     gene            3970..4650
                     /locus_tag="IQN08_15425"
     CDS             3970..4650
                     /locus_tag="IQN08_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="UOC45492.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSIASAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASPTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPD"
                     /name="IS6-like element IS1216 family transposase"
     gene            complement(4797..5444)
                     /locus_tag="IQN08_15430"
     CDS             complement(4797..5444)
                     /locus_tag="IQN08_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-8 chloramphenicol O-acetyltransferase"
                     /protein_id="UOC45493.1"
                     /translation="MTFNIINLETWDRKEYFNHYFNQQTTYSVTKEFDITLLKSMIKNK
                     GYELYPALIYTIVNIINQNKVFRTGINSEGNLGYWEKLNPLYTVFNKETEKFSNIWTES
                     NVSFNSFYNSYKSDLLEYKDKNEMFPKKPIPENTVPISMIPWIDFSSFNLNIGNNSRFL
                     LPIITIGKFYSKNNKIYLPVSLQVHHAVCDGYHVSLFMSEFQNIVDSVNEWI"
                     /name="type A-8 chloramphenicol O-acetyltransferase"
     gene            complement(5626..7065)
                     /gene="aph(2'')-Ia"
                     /locus_tag="IQN08_15435"
     CDS             complement(5626..7065)
                     /gene="aph(2'')-Ia"
                     /locus_tag="IQN08_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="UOC45494.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLES
                     LKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFIGE
                     PNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLPEHE
                     LHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNE
                     YIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTF
                     LTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIY
                     NDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGI
                     IDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIK
                     NIKQEFIENGRKEIYKRTYKD"
                     /name="aph(2'')-Ia"
     gene            complement(7066..7458)
                     /locus_tag="IQN08_15440"
     CDS             complement(7066..7458)
                     /locus_tag="IQN08_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="UOC45497.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCYT
                     LLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRACH
                     VYEKLGGKNDYKDEIVYVYDYEKGDK"
                     /name="GNAT family N-acetyltransferase"
     gene            complement(7565..7876)
                     /locus_tag="IQN08_15445"
                     /pseudo=""
     CDS             complement(7565..7876)
                     /locus_tag="IQN08_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277654.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            8074..8754
                     /locus_tag="IQN08_15450"
     CDS             8074..8754
                     /locus_tag="IQN08_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="UOC45495.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSIASAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASPTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPD"
                     /name="IS6-like element IS1216 family transposase"
     gene            complement(8809..9075)
                     /locus_tag="IQN08_15455"
                     /pseudo=""
     CDS             complement(8809..9075)
                     /locus_tag="IQN08_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /name="DUF4158 domain-containing protein"
     gene            9200..9805
                     /locus_tag="IQN08_15460"
     CDS             9200..9805
                     /locus_tag="IQN08_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="UOC45496.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERPE
                     FQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNEL
                     LDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRL
                     VFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
                     /name="recombinase family protein"
     gene            complement(9871..10503)
                     /locus_tag="IQN08_15465"
                     /pseudo=""
     CDS             complement(9871..10503)
                     /locus_tag="IQN08_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405587.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /name="peptide ABC transporter substrate-binding protein"
     gene            10792..11184
                     /gene="merR"
                     /locus_tag="IQN08_15470"
     CDS             10792..11184
                     /gene="merR"
                     /locus_tag="IQN08_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hg(II)-responsive transcriptional regulator"
                     /protein_id="UOC45482.1"
                     /translation="MIYRISEFADKCGVNKETIRYYERKNLLQEPHRTEAGYRIYSYDD
                     VKRVGFIKRIQELGFSLSEIYKLLGVVDKDEVRCQDMFEFVSKKQKEVQKQIEDLKRIE
                     TMLDDLKQRCPDEKQLHSCPIIETLT"
                     /name="merR"
     gene            11213..13108
                     /gene="merA"
                     /locus_tag="IQN08_15475"
     ARG             11214..13105
                     /name="merA"
                     /gene="merA"
                     /gene_name="mercury(II) reductase"
                     /tool="amrfinderplus"
                     /drug_class="MERCURY"
                     /identity="96.04"
                     /coverage="100.0"
     gene            13268..14590
                     /locus_tag="IQN08_15480"
     CDS             13268..14590
                     /locus_tag="IQN08_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="UOC45498.1"
                     /translation="MSDLLSLPDIKTIEPPQENETDMMFKVEAVGPPERCPECGFDKLY
                     KHSSRNQLIMDLPIRLKRVGLQLNRRRYKCRECGSTFWERLISVDEKRSMTKRLLKSIQ
                     EQSMSKTFVEVAESVGVDEKTIRNVFKDYVALKEREYQFETPKWLGIDEIHIIRRPRLV
                     LTNIERRTIYDIKPNRNKETVIQRLSEISDRTYIEYVTMDMWKPYKDAVNPILPHAKVV
                     VDKFHVVRMANQALDNVRKSLKAHMSQKERRTLMRERFILLKRKHDLNERESFLLDTWL
                     GNLPALKEAYELKEEFYWIWDTPDPDEGHLRYSQWRHRCMSSNSKDAYKDLVRAVDNWH
                     VEIFNYFDKRLTNAYTESINSIIRQVERMGRGYSFDALRAKILFNEKLHKKRKPRFNSS
                     AFNKAMLYDTFNWYEVNDHDITDNFGVDFSTPIKNLEKGDL"
                     /name="ISL3 family transposase"
     gene            complement(14717..16222)
                     /locus_tag="IQN08_15485"
     CDS             complement(14717..16222)
                     /locus_tag="IQN08_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="UOC45484.1"
                     /translation="MKVSDVISVILKSGIREYKCLNSKIKPIGYKAEGKKGAIFGFRSK
                     KLMTESRGIVLTSQEGLAENEDKFTHWTPNVYSYGAYADEKRIIVKGHNERNLQQINTF
                     VIDFDRLTGEKLDSQMILDAAIDLELMPTLILETPGGFQAYFILENAWYISSKNNYQSI
                     EVAKRVSENLRKAFAEELPSVDLGCNHFGIARIPRTDNIVYYYPALTHDIQQLIQWSMK
                     YEPAKTYKKPLLSLVKNKYKQVNEMWYRQLISNPKIVGAKGIMGRNNVIFTLSLANYAS
                     GVDLEDCLNEMDLFNSSLERPLKDRERVRIVKSAYSGKYRGASKTYVQELVSNWGLESL
                     SEEKMFTQQRNTWYKFKKERHKRKKSHLSEWKEDILAYLETQCYRYRPEVRLKKTDLQA
                     AITFNGQSIPKRSLDRALKELMAEGKLFVQIKAGRNGGLVVATRKALIRTVIQVKQQVK
                     SAYKQGIKTFFKEADMLVRIFREPDKYSQRVDHYKQLNLWNTG"
                     /name="primase C-terminal domain-containing protein"
     gene            16926..17816
                     /locus_tag="IQN08_15490"
     CDS             16926..17816
                     /locus_tag="IQN08_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="UOC45485.1"
                     /translation="MANTIIFGQQKGGVGKTTDTCMITLVAAMLMKKRTILIDIDLQAN
                     ATNFMTKTFGVETVPQTLMSALENGDLRPAIVNLKENLDLIPAGYDMRLYTEFLTENFD
                     NTIDRTFYLKNLIEEIKDDYDYIFIDIPPSTDLKVDNAMVACDYLVVVQETQQFSFEGS
                     QRLIFDYIQTLVDDFGSDVPIQIAGILPALLQQKRPMHQKLVEETIDTFGRDNVFNTII
                     QNHARLEWYPRIGFQFQDYHDKKMIALFADIFCELEERITLYKSNGDIQDYFYTPRYIL
                     DGKLTSLGKEISLNV"
                     /name="AAA family ATPase"
     gene            17809..18093
                     /locus_tag="IQN08_15495"
     CDS             17809..18093
                     /locus_tag="IQN08_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOC45486.1"
                     /translation="MSSLINRKIPQGATANKEADTKRKVTIQKKSSKKILASERKNIKV
                     SPSTFDLIKTISTMKSYKNYEFIDKAVESYIQNNLTEREQRILKNLISK"
                     /name="hypothetical protein"
     gene            complement(18126..18365)
                     /locus_tag="IQN08_15500"
     CDS             complement(18126..18365)
                     /locus_tag="IQN08_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010621028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOC45499.1"
                     /translation="MEQSDNVPLDRHKCKEHVFKDKRFVEKESESLTLLGYKIVVDTVT
                     GVNYLETPKGGITPLLDKNGKICIDKKKSINKKG"
                     /name="hypothetical protein"
     gene            complement(18761..18958)
                     /locus_tag="IQN08_15505"
     CDS             complement(18761..18958)
                     /locus_tag="IQN08_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="UOC45487.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCPK
                     CHRRYKQKQGEYVAITIIKE"
                     /name="transcriptional regulator"
     ARG             complement(5630..7065)
                     /name="aac(6')-Ie/aph(2'')-Ia"
                     /gene="aac(6')-Ie/aph(2'')-Ia"
                     /gene_name="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(4801..5444)
                     /name="catA"
                     /gene="catA"
                     /gene_name="type A-8 chloramphenicol O-acetyltransferase"
                     /tool="amrfinderplus"
                     /drug_class="PHENICOL"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(4798..5444)
                     /name="catA8"
                     /gene="catA8"
                     /gene_name="chloramphenicol acetyltransferase (CAT)"
                     /tool="rgi"
                     /drug_class="phenicol antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(5627..7065)
                     /name="AAC(6')-Ie-APH(2'')-Ia bifunctional protein"
                     /gene="AAC(6')-Ie-APH(2'')-Ia bifunctional protein"
                     /gene_name="aminoglycoside bifunctional resistance protein"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(7566..7849)
                     /name="rep_cluster_1118"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.17e-147"
     Typing          complement(14725..16222)
                     /name="rep_cluster_180"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.687"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tccagtaaaa acgacccttt gattcgttaa gtccattttc aaactctccc ttcccgtcta
       61 atagctgtta tatctatcaa gaaaagtggt gcaaggtatg ttcatattct ttcacccgtc
      121 gataaaaggt atttcgtttc atgcccaatt gatccatagc atagactgca gttatctttc
      181 ctgctttcca gtcagaatag atcgcaggaa attcaggagg taaagcaatt ttctttctgc
      241 cgaattttac acccttttgt ttcgcagctt ctacgccttc tctttgccgg ctattaattc
      301 gtttccgctc gtcttcggcc atatagctga gtaattgcag aattaagtca gagataagcg
      361 tctctaaccc atctagttgc ttgtaactag cagtatctaa cagaggcatg tctaacacag
      421 aaatatcaat ctgcttttcc ttagttaatt ctcgccattc ttctaaaatc atttgtttat
      481 ttctgcctag tctatctaat gattgtagat agagtaaatc tccttttcga aacatccgtt
      541 tcattaattg atattcaggg cgtttaaaat ctttgccgct ggctttttct acaaatatat
      601 attcttcagg gattcctaat tccttcgcgg aaatcagttg tcgttcgatc gattgatcat
      661 aactactgac ccggatataa aaaaattttc tcatcgtttt tatctaccct tttttcacta
      721 aaattgtttc aaaacaatca gattaaatca gaaacgtttc aaattgtatt atgtggttat
      781 tatgccatac ataaacaaag aaaatcaagg catgtcaaaa agtatacttt ttgaaacagt
      841 aagtttacac tcccgcatga ttatatttat tccgctaaaa tacataaaaa tggcaggcct
      901 taacggtctg ccattaaata aactagttat ccagtttttc ttttatgtct tccatggtat
      961 attttatttt tcacatcaga atttgacttc tttaactttt acaatttttt taacactatt
     1021 attctattac aacactatat atttctaatt tagggttaaa aataaatctt tttgatactt
     1081 ttgcctttac aatataattg tattctttct ttttttcttt gaagtcgctt gattcaactg
     1141 taacttcaaa ccacatttca tttggagact cttttaattt ttttacttga aatgaattat
     1201 ctcttggatc ataaggtggt actctttcaa actcaggaag ttttctctgt atttgttctt
     1261 caggagtatt cgcaaagtaa tctactatct tgacagttat taaaagtact actattccca
     1321 aaactataac aaacataaag ataattccac atccagaatt atttttatta ttttgagtca
     1381 gattattcca ttctatttct tccataatca tcatgtcctt ttacaattca tatacttatt
     1441 ttatcgtata tatttaaaaa ggaaaaggac actgtttaaa tagtaaacga ttcttaatta
     1501 agtttacttc tattttcttg aaattgaata attttttcag aaaatccatt tgcaactttc
     1561 ccctcatctc caataaaatc aagataagta aaatctgtcc caagtgaatt attaactatt
     1621 ttatcaatga aaaatgaaat tatccctaca cctaagatat caactgtttc gtcattttct
     1681 aataaactat ttgttactga agcgatttta tctgaagaac caaatctgtt cttaacaaat
     1741 tgattaaatc tatcaatagc acccttatca caatagtacg tttttaaagc cgataaagct
     1801 gttgaatcag aatttctttt tgctaaggta taaacattta ccgcgtcaac atcttggtat
     1861 aaatcttcta gactaaatgt tccacctgca gccgtaccta ctaatgcaaa tacaccttgc
     1921 tccatttgta ttcctaattt cttaacttca ccccagcaag tcattaaatc tcctaaccaa
     1981 cctgcaaagt cagaaacact atcattgatt gtgtcggcta gaaacatctg tgattgtaac
     2041 gtcacaatta agtgaggcaa acctattatt gaaccagtat taggatctat aaaataatta
     2101 tacaggccct cgctattagt taatttattc ctatattcat cataaatttt tctatcatgc
     2161 tctgttacag gagaagaaat gatgtcccat gacaaaccag aataatttac atatctataa
     2221 aatgtcaaat gagggttcca aggattatca agaccagctt gtgatagata ttgatacgca
     2281 cgtaccatct gatttataac cttctgattg tatttggcaa tgtaattatc attattagta
     2341 tcatttgata actcattaaa tccgctatct ctacccgatg tagcgattaa atcgatacca
     2401 acagctccgt tgccacttcc tatggtaaat tcaacaaatt gatcaaaaga ccattgtcta
     2461 ggcataggaa aacctaaatt cccactataa ccagtcgaca tatctgaaac aaatgcaaac
     2521 gaagcatacc cgttattgat tatttgagaa caaacatttc ttgttccgta aacacccgtt
     2581 tgatacccgc tatatgacat tacagcagat acccccttaa aatattctcc agcatatgca
     2641 agaatttctt cccctaacat gtctagatca acagcaaaat aaataactgt tgttaatggt
     2701 atatttagat tttttgctgc atctatagcc ttcttaccat ctgataagcc acgatcataa
     2761 gtaaaataag ctaatgctgc acctccatct tgatagatag gaaacacaga taaattttta
     2821 gaaaaaatat tattcaattc ctctgtagtt agatacttat ttcgttcatc ttttccaatt
     2881 ccaacagtac cagtaagata tctaccaact atttttatat cattatcaac caatgtttgg
     2941 atttgagatc tagttaattg cgtagaagta tctgcgccat ctgcagatct tctaatatct
     3001 ccagcgctag acaatagtcc ttttattaca acaacatctg ctatttcagt atattcaggc
     3061 aataccataa attttcggaa tgccagtact tctaaaccca tatatttagt gaaaatacca
     3121 tcgaatgtac cgtcttcaaa ataaccatta acatataatc catattgaat tattcttaca
     3181 tagtttcccg agttaccttc agacacagga taagttttac tagttgtccc attaccataa
     3241 gaccctgttg cttcactggg agaatatcct agttcagctt gtaatgcaaa aattaatgct
     3301 tgatttgaat ttctttgata aataccatca caaggcatta ttcccgtgta ttctaaataa
     3361 tttacattta accattgctg catttttcta attttactat ctccgccggg aacaagaaca
     3421 aaagcactca tatcaaataa cgctttggcc catttcgcat ccaaaactcc agttacagcg
     3481 cttccattaa accctgcatc agtctgcaac tcttttacag cagtatctaa atatggagta
     3541 tataccccat cgaagccacc tggattaatc cccttacacc aaaaggcacc ttgaataaga
     3601 aatacaatat ttttaggtag atttttgccc catttaggag taacctgatt atcgtacatt
     3661 ttttcagtgg taggtccaaa attagctact agattagtaa taccaagttc tacttgtagt
     3721 ccttcaataa gtccatagat tgtaggccac cctgttcgcc cattttctgg aaccttatca
     3781 aaccctgtaa cattactgta tttcttgttt agccatttct gagtacttaa aaccatctca
     3841 tccataaaaa tcccctgctt tcttgccgaa catacgttcg tttatgtgtt gattaggttc
     3901 tgttgcaaag ttttaaatct actatcaaat aaggtagaat aatagaaaaa gatagcagga
     3961 ggaatgacga tgaatcattt taaaggaaag caatttcagc aggatgtgat tattgtagcc
     4021 gtgggctact atcttcgtta taaccttagc tatcgtgaag ttcaagaaat cttatatgat
     4081 cgtggcatta acgtttctca tacgacgatt tatcgttggg tgcaagaata tggcaaacta
     4141 ctctatcaaa tttggaaaaa gaaaaataaa aaatcctttt attcctggaa aatggatgaa
     4201 acgtacatca aaattaaagg aaaatggcat tatttgtatc gagccatcga tgcagatggt
     4261 ttaaccttgg atatttggtt acgtaaaaaa cgggacacac aagcagccta tgcttttctt
     4321 aagcggttag tgaagcagtt tgatgaaccg aaggttgtag tcacagataa agcaccatca
     4381 attgctagtg cctttaagaa actaaaagaa tacggatttt atcaagggac agaacatcgt
     4441 accattaaat acctgaataa tttgattgaa caagaccatc gtccagtaaa gagacgcaat
     4501 aaattctatc gaagtttacg cactgcctca cccacgatta aaggcatgga agccatccga
     4561 ggattatata agaaaacccg aaaagaaggc actctcttcg ggttttcggt ctgtactgaa
     4621 atcaaggtat tattgggaat cccagattaa atcatagata ccgtaaggga ttttattctt
     4681 tatttaaaac tttgcaacag aaccttcaaa atcaaaacca caagcaacca aaaaatgtgg
     4741 ttgctataaa cttataaata tcataattac ttaagtttat gaaagttatt ttagatttaa
     4801 atccattcat ttacactatc aactatattt tgaaattcac tcataaataa tgaaacatgg
     4861 taaccatcac ataccgcatg atgaacttgc aatgagactg gtaaatagat cttattattc
     4921 ttactataaa atttacctat tgtaataatt ggcaatagga atctactatt attaccaata
     4981 tttaaattaa atgaactaaa atcaatccaa ggaatcatcg aaataggaac tgtgttttca
     5041 ggtattggtt ttttaggaaa catttcattt ttatctttat attcaagtaa gtcactctta
     5101 taactattat aaaaagaatt aaaactaaca tttgattctg tccaaatgtt agaaaatttt
     5161 tcagtttctt tattaaagac tgtatataaa gggtttaatt tttcccaata acccaaattt
     5221 ccctcactat taattcctgt tctaaatact ttattttgat ttataatatt tacaattgta
     5281 taaatcaaag caggatacag ttcatatcct ttatttttta tcatactttt aagtaaagtg
     5341 atatcaaatt ctttagtaac actgtaagtt gtttgttgat tgaaataatg attaaaatat
     5401 tcttttctat cccaagtttc caaattaata atattaaaag tcatatataa ttttcctcct
     5461 aaatttcatc ttatctcttt gtatttagga ggaataatcc tctgattttt tcatatattg
     5521 tgtcacctct taaacgttaa ttatactaaa gcctttatta tttttcattt ttttaaacta
     5581 tttaatacta atgtctttta taatagcttt tcatatatta tataatcaat ctttataagt
     5641 ccttttataa atttcttttc taccattttc gataaattcc tgtttaatat ttttaattcc
     5701 ataaacaata gtttcaatag gataatattc ttcaactata tcttgatatt cttttgcttt
     5761 ctcaatatct atatttccat acattcttaa tatatcttct ccaaaatttg ttcctatttc
     5821 ttcttcacta tcttcaagta agtatataaa atcacaatat tcatctataa ttccagaatc
     5881 tccaaaatca attattccag ttaatctatt attgccatct aacaatagat gattacaact
     5941 aaaatcatta tggcataaac actttttacc ctcaaaaact gttgttgcat ttagtctttc
     6001 cataaaactt tctatataat ctttttctat atcagttaaa tcattataaa tagtttcacg
     6061 caacaatata tactcttcta atacattttg tttattatca atagtacatt cactaatatc
     6121 tgtataatct aaaccgtgca tttgtcttaa aaaactggca atatctcgtt ttaacaaatt
     6181 ttgttcttct tctgacatag tagaataaat ttctggtgtt aaaaaagttc ctttaatttc
     6241 tttataacct agtatagata attcatcact aatatacgaa tattcaatat taggaatttt
     6301 tacattagtt tctaaatttg tatttaaaaa attatatatt gctttttctt ttgcataacc
     6361 ttttttctta ttagtactaa attttgtttt aaaaatgtat tcattattaa ctaaatatgc
     6421 cacactatca taaccactac cgattatttc aatactatct actttgaaat tatcaaagta
     6481 atgctcaatt aaatatttca ttgccttaac atttgtggca ttatcatcat atctatattc
     6541 cattaaataa caatcttctt ttttgccctc gtgtaattca tgttctggca aatcttcaat
     6601 aattctaaaa ccagattttt ggtatgccct tattgctctt ggattatttt tatgagggtc
     6661 taaaataact gcattagcat ttctttcttt tttcaaaaat tcaaaaatca atttaatata
     6721 tcttgtacca attcctttac tccaataatt tggctctcct ataaattgat ccataccata
     6781 gactatctca tcagtttttg gataatgata atcagtatat aactcatcat acattttata
     6841 tatttgtcca tatccaatag gaacattgtt atattcaata attactctaa aaacttcatc
     6901 ttcccaaggc tctgtataat gtttttttaa tgattctaat gtatattttt tatctctacc
     6961 accataaaat tctaatactc tttcatcagt taaccatttt aacatcaaag gaaaatcatc
     7021 atctattaaa gttcttatac atatttcatt ttcaactata ttcatttatt tatcaccttt
     7081 ttcataatca tatacatata ctatttcatc tttataatca tttttaccac ctaatttttc
     7141 atatacatgg caagctctag gattaccttt atcagttatt aaaaacattt cagaacaacc
     7201 aatctcttta gaatattcct taataaaaga taataatttt gaaccataac ctttgtcttg
     7261 atagttaggt aacattccta ttgagtgtaa ataaaacatt gtttttccat caggtcttaa
     7321 aagtgtatag caatatgcaa atcctataat tttattattt tctttagcta taaacccaaa
     7381 tgaactggta tcattaagaa atccttttaa attatcaata tcaaaaacca tattatcatc
     7441 aattaaaact tcttccataa actcagtcaa gtccagactc ctgtgtaaaa cattgtatag
     7501 tattttacac aggattatgg acttgactat actaaagtaa cattttgtat aaaacaaagt
     7561 tactttactt ccaaaatcta aattcacgtt gccaaaaatc aatctgcttt tgcaattgtt
     7621 tttcgttcgc ttttaaagtc gatttcatta aatcagttaa atcaattaga gatatttctt
     7681 taattaattt tttatattta ttcttagtac gtctttctaa ttttccccat tcttcttcat
     7741 cattcaacaa cataaaaacc attgctctat cagaagtacg ttcgatagtt ttccaatctg
     7801 gttgtaatat atgtaaatca ttaaaacaat cgttccaata atcaaccata tctcttttta
     7861 attcaatttt tatttttatt atttttaatc tctttaaata tctttacgca cgtatataga
     7921 cttcataatt ttaaaattat atagacttca caacacaata tttcggtaca acttccatgt
     7981 aaagtataac acactagacg gttctgttgc aaagttttaa atctactatc aaataaggta
     8041 gaataataga aaaagatagc aggaggaatg acgatgaatc attttaaagg aaagcaattt
     8101 cagcaggatg tgattattgt agccgtgggc tactatcttc gttataacct tagctatcgt
     8161 gaagttcaag aaatcttata tgatcgtggc attaacgttt ctcatacgac gatttatcgt
     8221 tgggtgcaag aatatggcaa actactctat caaatttgga aaaagaaaaa taaaaaatcc
     8281 ttttattcct ggaaaatgga tgaaacgtac atcaaaatta aaggaaaatg gcattatttg
     8341 tatcgagcca tcgatgcaga tggtttaacc ttggatattt ggttacgtaa aaaacgggac
     8401 acacaagcag cctatgcttt tcttaagcgg ttagtgaagc agtttgatga accgaaggtt
     8461 gtagtcacag ataaagcacc atcaattgct agtgccttta agaaactaaa agaatacgga
     8521 ttttatcaag ggacagaaca tcgtaccatt aaatacctga ataatttgat tgaacaagac
     8581 catcgtccag taaagagacg caataaattc tatcgaagtt tacgcactgc ctcacccacg
     8641 attaaaggca tggaagccat ccgaggatta tataagaaaa cccgaaaaga aggcactctc
     8701 ttcgggtttt cggtctgtac tgaaatcaag gtattattgg gaatcccaga ttaaatcata
     8761 gataccgtaa gggattttat tctttattta aaactttgca acagaaccga taatttctcc
     8821 aggatcaata tttagttgag ttgctacata actcaataca ttttggggaa tattggtaag
     8881 atctaatata aaagttccta aaaaacgtag tgagactaac tgtagtgcaa aacctaatct
     8941 agtagctggt cgtcgtatag tctccatcaa ttctaaatct ttttcatgca aataaaaaaa
     9001 ttggtttaat tgttctgtac taggttctcc taaataaatg ccaaatgctt ttttgttact
     9061 tttgatttca tccatttaaa ccctccatga agtaaatttt aagtctacat aaaatttatt
     9121 tttaaaaatg ctatgatagc agtaaaacta caagtaaatt ttacatgtaa attatatcac
     9181 ttttaaaaag gagtttttta tgggaaaaat tggctatatt cgagtatctt cacaagatca
     9241 aaatttggat cgacaattgg caatgatgga aggacttaac attgataaat tattccaaga
     9301 aaaagccagt ggtaaagata ctgagcgtcc tgagtttcaa aaattactaa attatatccg
     9361 tgaaggtgat tgcgtcatcg ttacatcact agatcgttta ggaagagatt atgaagatat
     9421 aaaaaataca gttgctttta tgaaacagaa aaaagtagcg ttaaaaatat tagatgcaca
     9481 gtttttagat tttaatactg gaaatgaact cctggatacc gctatgttcg atatgttttt
     9541 atcactgcta agttatattg cacaaaacga gcgtgagaaa attcgtgaac gtcagcggca
     9601 aggagtatta ttggctcaaa aagccggccg atataaggga cgaccaatcg agtattctat
     9661 taactcaaca gatccgcaaa aacgcttagt ttttaaaacc gtggtggaca tgttaaagca
     9721 aggcattcca gttgcagaga ttgcaaaaga aaacgggatc tctcgaccaa ctatttataa
     9781 aataaagaaa gcgaatgatt tgtagaaata tactacattc attcgctttt ctaccgcact
     9841 atttcgtttc tatgttccat gtaccccgat atagggtcca ttataaatca cactatcaga
     9901 gttcgtacca tatctatctc cttctttttc aacaacttcc tgtttttgag gaaaaaatgc
     9961 tgtattaatt agcatagaag tgaaatatgg gattggcgta ctcaaattaa taaccaaagt
    10021 attcttatcc ttagcataaa ctcccaactt atctggtttc atagttcctg agattatttc
    10081 tttaccattt ttaatagatt caaaaaaacc aatatttggt gaatcttgat tacttaacgt
    10141 tcttttccac gaataaacat agtcatctgc aacaacaggc gtaccattac tccacattgc
    10201 atcctttctc aatcgaataa cgtacttaag tccatcctta gagatttctg gaaggccatt
    10261 agctcctcca ggtacaattt cacctttagc attaagttta tataatcctt ccattacgtt
    10321 atttaaaaca agttggcttg tagggtcgat agctaacttc ggatctaagc tttgtactgt
    10381 agtagaaaca gcaaaagtat aagctttggt ttcttttgct gtttcttgag acttttcccc
    10441 acaaccagta ataattattc ctaacataag aattgttaaa ttctttcccc aagttctttt
    10501 caaaaaaaca cccctattca atttcttatc tgattgttct agcaacactt agcttaatta
    10561 tactgtaaaa aaaacgggtc ttcggaatat atagtggaat tactttctta atagtttaaa
    10621 aacaactggt gtttaaatcg gattttctgg tgggatgtta ggataatcaa tagtattata
    10681 taaaagtatc aatcacatac gagtgatact ttgggaaaaa aataaaatag tccaatttac
    10741 ttgaacgtgt actatggtac acggtttata atattcatgg ggtgaaataa gatgatttat
    10801 cgcattagtg agtttgcaga taaatgtgga gttaataaag aaacgatcag atattacgag
    10861 cgaaaaaatt tattacaaga acctcaccga acggaagctg gttatcggat atattcatat
    10921 gatgacgtta agcgtgttgg gtttattaaa cgaatacagg aacttggttt ttctttaagc
    10981 gagatttata aattacttgg tgttgtagat aaagatgaag ttcgttgtca agatatgttc
    11041 gaatttgttt ctaaaaaaca aaaggaagtg caaaaacaaa tagaggattt aaaacgaatt
    11101 gaaactatgt tagacgactt aaaacaacga tgtccagatg aaaagcaatt acattcgtgt
    11161 ccaataatag aaacattaac atgagagatt aattaacgaa aggagctttt ttatgaataa
    11221 atttaaggta aacatttcag gaatgacttg tacgggttgt gaaaaacacg tagaatcagc
    11281 acttgaaaag atgggtgcta aaaatattga gtctagttat cgtcgtggtg aagcagtatt
    11341 tgaactgccc gatgatattg aggttgaaag tgcaataaag gcgattgctg acgcaaacta
    11401 tcacccgggc gaagcagaag aatttcaatc ggaacaaaag acgaatttat tgaaaaaata
    11461 tcggctaaac gttgaaggaa tgacctgcac tggttgtgaa gaacatattg cggttgctct
    11521 tgaaaatgca ggtgcaaaag ggattgaagt agactttcgt cgcggagaag cactgtttga
    11581 actaccgtat gatgtagaca ttgatatcgc gaaaacagcg attactgacg cacaatatca
    11641 accgggcgaa gcagaagaaa tacaagtgca atcggaaaaa aggacagatg taagtttaaa
    11701 tgatgaaggt aactatgatt atgattacat catcatcggt tctggtggag ctgccttttc
    11761 atctgccatt gaagccgtta ctttgaacgc aaaagtggct atgattgagc gtggaacggt
    11821 gggtggaact tgcgttaatg tcggatgcgt tccttctaag accttattaa gagcagggga
    11881 aatcaatcat ctagcaaaaa ataatccatt tgtgggatta cacacttcgg cttcaaatgt
    11941 tgatttagcg ccattagtaa aacaaaagaa tgatttagta accgagatgc gaaatgaaaa
    12001 atatgtgaat ttaattgatg attatggttt tgaattaata aaaggtgaag caaaattcgt
    12061 aaatgaaaat acagttgaag taaatggcaa tcaaatcaca gccaaaagat ttttaatagc
    12121 tacaggtgct tcttcaactg cacctaatat tcccggatta gatgaagtag attatttaac
    12181 aagcactagc ttattggaat taaagaaggt tccaaatcgt cttaccgtaa ttggttcagg
    12241 atatatcggc atggaattag gacaactatt tcataacctc gggtcagaag tcactttgat
    12301 tcaaagaagc gagcgtctat taaaagaata cgatcctgaa atttcagaag ccattactaa
    12361 ggccttaaca gaacagggaa ttaatttagt aacaggtgca acctatgaac gagttgagca
    12421 agatggagac attaaaaaag ttcatgttga gataaatggt aaaaagcgaa ttattgaagc
    12481 agaacaattg ctaattgcca ctggaagaaa accaaataca gaatcattaa acttacatgc
    12541 agcaggcgtt gaagttggtt cccgtggtga aattgtcatt gatgattatc ttaaaacgac
    12601 caattcccga atttattcag ctggagatgt cactctcggt ccccaatttg tttatgtagc
    12661 tgcttatgaa ggtggacttg ctgctcgtaa tgcaatcgga ggactaaatc aaaaggtcaa
    12721 tttagaagtg gttccaggcg ttacgtttac ttctccatcg attgcaacgg ttggtttaac
    12781 ggagcaacag gcaaaagaaa aaggatatga agtgaaaaca tcggtattgc cgttggatgc
    12841 tgttccaaga gcgctcgtta atcgggaaac aacaggtgtt ttcaaattag tggcagacgc
    12901 gaaaacattg aaagtgttag gggcgcatgt agtggcagaa aacgcaggag acgtaattta
    12961 tgcagcaaca ttagctgtga aattcggttt aactgttgga gatctgagag aaacgatggc
    13021 tccatatcta acaatggcag aaggattgaa gctggctgtc ctaacttttg ataaagatgt
    13081 ttcgaaatta tcttgctgtg caggctaagg gaactttttt acaactccct attcaagtga
    13141 accagctaat gctttaaggg attttctaag tgcatttgtg gtcacaaaaa taatttaaca
    13201 ctgatgattt cgacatcaaa tctataattg ataccttaac accgcagagt aataaaggat
    13261 gaataatatg tcagacttat tatccctacc agacattaaa acaatagagc cgccacaaga
    13321 aaatgaaacc gatatgatgt ttaaagttga agcagtcgga ccacctgaac gttgtcctga
    13381 atgtggtttt gacaagttgt acaaacacag ttcaagaaat caactaatta tggatttgcc
    13441 cattcgttta aagcgagtgg gcttacaatt gaaccgtaga cgatacaagt gtcgtgaatg
    13501 cggatctacc ttctgggaac gcctaatatc tgtagatgag aagcgtagta tgaccaaaag
    13561 gcttttaaag tccattcaag agcaatccat gtctaagacc tttgtagaag tcgcagaaag
    13621 cgttggtgtt gacgagaaaa ccattaggaa cgtttttaag gactatgtgg cactcaaaga
    13681 acgtgaatac cagtttgaaa ctcctaagtg gcttgggata gacgagatac atattatccg
    13741 tagacctcgg cttgtattga ctaatattga acgcaggact atttatgaca tcaagcctaa
    13801 ccgtaacaag gaaacagtca tccaacgtct ttcagaaatc agtgacagga cttacattga
    13861 gtacgtcaca atggatatgt ggaagcccta caaagacgca gtgaacccta tccttccaca
    13921 cgctaaagtt gtcgtagata agtttcatgt agttagaatg gctaatcaag ccttagataa
    13981 cgtcaggaag tctttgaaag cccatatgag ccaaaaagaa agacgtaccc ttatgcgtga
    14041 aaggtttatc cttctaaagc gtaaacacga tttaaatgaa cgtgaatcat tcctcttaga
    14101 tacttggtta ggtaatcttc ctgctttaaa agaagcctat gaactcaaag aagagtttta
    14161 ctggatatgg gatactcctg atccagatga aggtcatctt cgttatagtc aatggagaca
    14221 ccgttgtatg tccagcaact ctaaagacgc atataaagac ctcgtgagag ccgtagacaa
    14281 ctggcatgtc gaaatattca actactttga taaaaggctc actaatgctt atacggagtc
    14341 aattaacagc attattaggc aggtagagcg aatgggtaga ggttactcgt ttgatgcctt
    14401 acgagccaaa atccttttca atgagaagct ccataaaaag cgtaagccac gatttaattc
    14461 aagtgctttc aataaagcta tgttatacga tactttcaat tggtatgaag tgaatgatca
    14521 cgacattaca gacaactttg gtgtcgattt ttccacacct attaagaatt tggagaaggg
    14581 tgatttataa gcccttttcc accataaaat ccgaataccc aaaaaaacaa aaatatacag
    14641 aattaggata ataactaaca aaaacgaaaa caagtataaa agaaatataa tgtataggca
    14701 cttgacatgt tttattttat cctgtattcc acaaatttaa ttgcttataa tgatctactc
    14761 tttgactgta tttatccggt tctctgaaga ttcttactaa catatcggct tctttaaaaa
    14821 atgtcttaat gccctgtttg tacgcagatt ttacctgttg cttcacttga atgaccgtac
    14881 gaattaacgc tttacgagtg gcaactacta gtcccccatt ccgtccagct tttatttgta
    14941 caaataattt accttcggcc atcaattctt ttaatgcacg gtcaagagaa cgtttaggaa
    15001 ttgattgtcc gttgaaagta atcgctgctt gcaaatctgt tttcttcagt ctgacttcag
    15061 gtctgtatct ataacattgg gtttcaaggt aagctaaaat atcttctttc cattcggaaa
    15121 ggtgagattt ctttctttta tgacgttcct tcttaaattt gtaccaagta tttctttgtt
    15181 gtgtaaacat tttttcctca gacagcgatt ctaagcccca atttgacact aactcctgta
    15241 cataagtctt acttgcacct ctatacttcc cagagtaagc actttttaca attcttactc
    15301 gttctctatc ttttagagga cgttctaaag aactgttaaa aagatccatc tcatttagac
    15361 agtcttctaa gtcaactcct gatgcataat tggccaaaga taaagtgaaa atcacattat
    15421 tccggcccat aatcccttta gcgcctacaa tttttggatt actaattaat tgtcgatacc
    15481 acatttcgtt cacttgttta tatttatttt taacaagtga aagtagcggt tttttatatg
    15541 ttttcgctgg ctcatatttc attgaccatt ggattaattg ctgtatatca tgtgttaaag
    15601 ctggataata atagacaatg ttgtcagttc tagggatacg tgctatccca aaatgattgc
    15661 aacctaaatc gactgaaggc aattcttcag caaatgcttt tcttaagttc tcagatactc
    15721 gtttggccac ttcgattgac tgataattat ttttggacga aatataccag gcattttcaa
    15781 gaataaagta tgcttggaag ccccctggtg tttccaaaat aagggttggc attaattcca
    15841 gatcgattgc tgcatcgaga atcatttgag aatctaattt ttcgcctgtt aagcgatcaa
    15901 aatcgattac aaaagtattg atttgttgca agttgcgttc gttgtgccct tttacaatta
    15961 ttcttttttc atctgcatat gcaccatagc tgtaaacgtt aggtgtccaa tgagtaaatt
    16021 tatcttcatt ttcagctaag ccttcttgag aagtaagaac gattccacga gattccgtca
    16081 ttaacttctt ggatctaaac ccaaaaatag cacctttttt tccttcagct ttatagccga
    16141 ttggtttaat tttgctgttt aaacacttgt attcacgaat accacttttt aaaataactg
    16201 aaatcacgtc agaaactttc atcttatcac cacccattct atatttaaaa aaaggagaac
    16261 tgcaggcatt ctacagctct cctttaaacc ttatttgcag ctaaaatata gctcaaaatc
    16321 ttgattttta aggtcccaaa tgatataatt aaaaagacta agagagctgc aactctctgt
    16381 aatctaaaac tataatggga cgaaagagaa tcatgaaatg ctgtgtacat tttgattctg
    16441 taatttagtt gatcggcaaa tcaactaagt taccgtctct atttttttgt ctataattca
    16501 attagtaaat gtatcttttt tctattcatt caaattcttc tttagaatac atgtcactac
    16561 aacttttgta aagtaaaaat tttctctcaa ataatcattc ttaatttatt tttgtatcct
    16621 tttatctttg tatcctttta tctttttatc tttttatctt tttatccttt aattttattg
    16681 ttgtatcttt ttatctttgt atccttttat ctttttatct ttgtatcctt atttagaagt
    16741 ctatttaata acttttctta taaaatgcta tctgattaaa taagtatgtt ttaagataat
    16801 tagaaaaaag atacatttat ctatttacaa ttaattctat ttgtattact atttaaatat
    16861 agaaaaaaga tacaaagata catttatctt ttattacatt ttgattacaa ggaaggagta
    16921 tattgatggc taatacaatt atatttggac aacaaaaggg cggggtagga aaaacaactg
    16981 acacttgcat gataacgttg gtagctgcga tgctaatgaa aaagcgtact attttaattg
    17041 atattgattt gcaagcaaat gcaacaaatt ttatgacaaa aacatttggg gtagaaacgg
    17101 tacctcagac tttgatgtct gctcttgaaa atggcgatct acgtcctgct atcgtaaacc
    17161 ttaaggaaaa cttagattta attcccgctg gttatgatat gcgtctatac actgaattct
    17221 taacagaaaa ttttgataat acaatagata gaacttttta tcttaaaaac cttattgaag
    17281 aaattaaaga cgattatgat tatattttta ttgacatccc accttcaacc gatttaaagg
    17341 tagataatgc tatggttgca tgtgattact tggttgtagt acaagaaaca caacaatttt
    17401 cttttgaggg aagtcaacgg ctaatttttg attatataca aaccttagtt gacgattttg
    17461 gctcagacgt tccaattcaa attgcaggca ttttacctgc attgttacaa caaaaacgcc
    17521 caatgcacca aaaattagtt gaggaaacaa tagacacatt tggaagagac aatgttttta
    17581 acactattat tcaaaatcat gcacgtttag agtggtatcc acggattgga tttcaatttc
    17641 aagactatca tgataaaaaa atgattgctc tttttgcaga tattttctgt gaattagaag
    17701 aacgcatcac tctttataaa agtaatggtg atattcaaga ttatttttat actccgagat
    17761 atatattaga cggaaaatta acttccctgg gaaaggagat tagtctaaat gtctagtttg
    17821 ataaatcgta aaatcccaca aggggcaaca gcaaataaag aagcagatac aaaacgaaaa
    17881 gttactattc aaaaaaaatc gagtaaaaag atattagctt cagaaagaaa aaacatcaaa
    17941 gtgtccccct ctacttttga cttaattaaa accatatcaa caatgaaaag ctacaaaaat
    18001 tatgaattta ttgataaagc tgtagaaagc tatatacaaa ataatttaac agaacgtgaa
    18061 caacgtatcc tcaaaaattt aatctcaaaa taaataaaaa aggataaaaa gatacatgta
    18121 tctttttatc cttttttatt tattgatttc tttttatcaa tgcagatttt accattttta
    18181 tcaagtagag gcgtaattcc tcctttagga gtttctaaat aattcacacc agtaacagta
    18241 tcaactacaa ttttatagcc taatagagtt aaagattcac tttctttttc aacaaaacgt
    18301 ttgtctttaa atacatgttc cttacattta tgtctgtcta atggtacgtt atctgattgt
    18361 tctatcaact cactatttga attatccccg gcaaccatca tgcctaaatt tttatcataa
    18421 taaatatatt ctccacctag tattaaggtt aatggcataa aaatcttcct ttctttaatt
    18481 ttcttaccgt cctttttctc tttttttaac ggctttcgtt acttaccttt ttgttcattt
    18541 tctacgcaca atcaatcgat taaacagatt gcctcttatt ccaataagta aatttcaagt
    18601 gcaaatcatc ctatttttaa ataggatctg ttgtcaactt tttcttaatt ctatcaaaaa
    18661 acggaagatt ttgcttgcaa accaattccc ttttactaaa ttaaaatagg tttttattta
    18721 gtatgaaaca gaaaccaaca ttgaagtaaa tcttatttgt ttattcttta attattgtaa
    18781 ttgctacata ttctccttgt ttctgtttgt atcggcgatg gcatttagga cagtaaaagt
    18841 aatttttttg ataaacactt tttgctaatt ccccacagtc acactccacg tgaaaagcaa
    18901 tatttcgatt aaaactagtt atttcaatgc cccaacgatc ataagatttt cgcttcataa
    18961 aaattttcct ccttgtaaat tttactaacg attataaata ccgttgccat tctttcaact
    19021 gttgactaat taacgcataa aattcgactt ctgataaaat ggtaatctca tgacctaacg
    19081 caatcaagtc ccgcgcggtt tgttcctttt ttgtatagag agaatcatca aacaacgact
    19141 tagaatactg accacaaacc aggaaatttg tttttgaagt aacacttgat tgacaatggc
    19201 cgtttaaaga gtgaaccaaa ccgatggctt gggttcgtgt catttgctgc agcgt
//
