LOCUS       CP063596               11366 bp    DNA     circular BCT 04-APR-2022
DEFINITION  Enterococcus faecium strain BMT-1-3-1 plasmid pIN-3-1_03, complete
            sequence.
ACCESSION   CP063596
VERSION     CP063596.1
DBLINK      BioProject: PRJNA669626
            BioSample: SAMN16483158
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11366)
  AUTHORS   Both,A., Kruse,F., Mirwald,N., Franke,G., Christner,M., Huang,J.,
            Hansen,J.L., Kroger,N., Berneking,L., Lellek,H., Aepfelbacher,M. and
            Rohde,H.
  TITLE     Population dynamics in colonizing vancomycin-resistant Enterococcus
            faecium isolated from immunosuppressed patients
  JOURNAL   J Glob Antimicrob Resist 28, 267-273 (2022)
   PUBMED   35134550
REFERENCE   2  (bases 1 to 11366)
  AUTHORS   Both,A., Kruse,F., Christner,M., Huang,J., Mirwald,N., Franke,G. and
            Rohde,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) Institute of Medical Microbiology, Virology
            and Hygiene, University Medical Center Hamburg-Eppendorf,
            Martinistr. 52, Hamburg, Hamburg 20246, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Trycycler v. v0.3.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 86.6x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/27/2020 17:56:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.13
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 2,993
            Genes (coding)                    :: 2,718
            CDSs (with protein)               :: 2,718
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 275
            CDSs (without protein)            :: 275
            Pseudo Genes (ambiguous residues) :: 0 of 275
            Pseudo Genes (frameshifted)       :: 196 of 275
            Pseudo Genes (incomplete)         :: 65 of 275
            Pseudo Genes (internal stop)      :: 33 of 275
            Pseudo Genes (multiple problems)  :: 18 of 275
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..11366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="BMT-1-3-1"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pIN-3-1_03"
                     /geo_loc_name="Germany: Hamburg"
                     /lat_lon="53.33 N 10.1 E"
                     /collection_date="2014-07-22"
                     /collected_by="Institute of Medical Microbiology, Virology
                     and Hygiene, University Medical Center Hamburg-Eppendorf"
     gene            1..933
                     /locus_tag="IQN10_15345"
     CDS             1..933
                     /locus_tag="IQN10_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UOE11772.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIRF
                     YFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIHGE
                     VDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWAEFS
                     KEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFTW
                     KAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEADFKKQQ
                     QTEKEEAEKQSISNELKQDLLENLQNLFD"
                     /name="replication initiation protein"
     gene            950..1420
                     /locus_tag="IQN10_15350"
     CDS             950..1420
                     /locus_tag="IQN10_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="UOE11773.1"
                     /translation="MIDKTIKELAEEFHVSKQAIRKRLSADFRENYVQTVTRNGVKTLV
                     VSYKGYLLLKQHFLGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLDQQ
                     QQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKENNNAITTEKKWWKFW"
                     /name="DUF536 domain-containing protein"
     gene            1504..1808
                     /locus_tag="IQN10_15355"
                     /pseudo=""
     CDS             1504..1808
                     /locus_tag="IQN10_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /name="helix-turn-helix transcriptional regulator"
     gene            2083..3265
                     /locus_tag="IQN10_15360"
                     /pseudo=""
     CDS             2083..3265
                     /locus_tag="IQN10_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313302.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /name="FtsW/RodA/SpoVE family cell cycle protein"
     gene            complement(3494..4642)
                     /locus_tag="IQN10_15365"
     CDS             complement(3494..4642)
                     /locus_tag="IQN10_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="UOE11774.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="transposase"
     gene            complement(4659..5063)
                     /locus_tag="IQN10_15370"
     CDS             complement(4659..5063)
                     /locus_tag="IQN10_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="UOE11775.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="IS200/IS605-like element ISEfa4 family transposase"
     gene            5868..6041
                     /locus_tag="IQN10_15375"
     CDS             5868..6041
                     /locus_tag="IQN10_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOE11767.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNRELKLNSNYYNS
                     PNEELFVKQTDK"
                     /name="hypothetical protein"
     gene            6445..7599
                     /locus_tag="IQN10_15380"
     CDS             6445..7599
                     /locus_tag="IQN10_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="UOE11768.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQPI
                     EEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDA
                     DNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIK
                     LLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAIKRVKSVSSGDNFIMI
                     NWRIAETQTSFPWCAAYVEAAILCNKTDVHATSARAIMHYTYPNLNNSQIESKSLNQDQ
                     IIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQ
                     GGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
                     /name="peptidase C47"
     gene            7593..8015
                     /locus_tag="IQN10_15385"
     CDS             7593..8015
                     /locus_tag="IQN10_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOE11776.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDHS
                     ERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNKKQ
                     LDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
                     /name="hypothetical protein"
     gene            8317..8697
                     /locus_tag="IQN10_15390"
     CDS             8317..8697
                     /locus_tag="IQN10_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UOE11769.1"
                     /translation="MSEQNQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            8679..9593
                     /locus_tag="IQN10_15395"
     CDS             8679..9593
                     /locus_tag="IQN10_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="UOE11770.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            9833..10522
                     /locus_tag="IQN10_15400"
     CDS             9833..10522
                     /locus_tag="IQN10_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOE11771.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQVLAQKIEKFTACY
                     EDMNDIFVKQEKRLTDKNMETLTRLYRNLPEEQQKEYQEKYEQVKKDVQYVKDTKVEEA
                     CSDLFGDTIPFSTSEQERKERQQSVTYERYEILLQQAINIQNLTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(10644..10739)
                     /locus_tag="IQN10_15405"
     CDS             complement(10644..10739)
                     /locus_tag="IQN10_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="UOE11777.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     Typing          2..933
                     /name="rep_cluster_1249"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8680..9590
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggcaaatg aaatcgtaaa gtatgaacca gaattaaata ccattccttt aagaaaattt
       61 accccaatag aaatgaactt gtttttttct attgtctcta gaatgcgcga taaaggtaat
      121 caaaaaatac gcttttattt cagccaactt aaagatttaa gtgcgtataa gcagactgca
      181 ggagaagtct ttataaaaga tttggcaaga acatacgatc atttgatgga tttacgtttc
      241 ggaagaaaaa gcaatggtgg attaacaata gaacgattta caatgtttac tgattttgaa
      301 attcatggag aagtcgataa cccttatgta gatataaaag tccacgaaaa agctttgcct
      361 ttacttaata atctagaaag ctgggtacgt ttctctttaa cagaatttcg agatttaaat
      421 agcagttatg ctaaaacaat gtttagattg ctaaaacaat accgaactca aggatgggct
      481 gaattttcta aggaagattt tttagagcta ttagatattc ctaaaagcta tagacaaaca
      541 aatataaatc agtttgtact caaaccaata aaagaagaac taactccact atttaaagga
      601 ttaactattc gtaaaaaata tggtaaaggt cgaggtaaac ctgttattgg ttatcgtttt
      661 acttggaaag ctgaaatcaa ccatgctgac gatttttcaa aagggaagca agaagattta
      721 cgtataaaat tatttaatat tgaacacaat ggagaattaa cacaaaaaga aaaatggcgt
      781 gctaaagatc gaatactaaa tcttccgtta ggtactcatg aagctgattt taaaaaacaa
      841 caacaaaccg aaaaagaaga agcggaaaaa caatcaatct caaatgagct taaacaagat
      901 ttattagaaa acttacaaaa cttattcgat tgaagcataa ggagtattta tgatagataa
      961 aacgatcaaa gaactcgcgg aagaatttca tgtttcaaaa caagcaatta ggaaaaggct
     1021 atcagctgat tttagagaaa actatgtgca aactgttact aggaacggag tgaaaacctt
     1081 agttgtcagc tacaaaggat atttgctttt aaaacaacat tttttaggta gtaacaccac
     1141 tggtaaccaa gtagaaaccg ttgctagtaa cgatgaaaac tcgattattg tttttttaca
     1201 tgaacagctc caagaaaaag atactcaaat aaaacaaatg caaaagttac tcgaccagca
     1261 acaacaactc accttgcagg caaatcaaca gatcgaaaaa ctgcaagaac agttacaatt
     1321 agcttatacg gaagaacctg aagaagagga attaacttct gtcaataaag aaaacaacaa
     1381 tgcaataaca acagaaaaaa agtggtggaa attttggtaa caattaccaa ctttccataa
     1441 agaagaaatc aatttctata gactctatct ttctaagaat aaaaaatatg tttaggtgat
     1501 caaatgacta taagtaaagt aatcaaacaa aaaagaatag aaaaacaact aactcaagaa
     1561 gatttagctg aaatgctttt agtatctaaa aaaaccattt ctaattggga aaatggtcga
     1621 acaataccag atacagaaaa tttaattcag tttgctaagt tatttgattt atcactagac
     1681 aatattttgt taggagaaga aaaaattatg gtaaaaaatc atacaaaaac actaatacta
     1741 ttatttcatt ttgtaatttt tttaagttta gttttcacct taattcagtc cggattcttt
     1801 tattttagcc attcaattgc cacttggtta ttaatatttt ttctaagttt actattaata
     1861 attaatactt taattattat ctttaataaa catagaaact tatttcttca cagcatattt
     1921 ccaattgttt tattaatttt tctatacttt atataaaaag atagaactat ttagtaagat
     1981 aaaaaagtag ctaattgtaa ttttcgtcta aaactcaaaa ctagtaaaaa taactttatt
     2041 gtgttatttt tatttataaa ttatgaaaaa ggacctttcg aaatgaaaaa taatagaatc
     2101 tacaaaaatc agttgaatta tgggctttta ttgccagttt ttctgctatg tatcattgga
     2161 ttactttctt tatatgttgc tttatatcac gactcttaca ctaattcaaa aatcacaaaa
     2221 gaattaatta aacaagtatt gtggtatagt attgggttca cttcagcaat aactatttat
     2281 ttttttaatc ctaaaatttt gtggaaatta actcctatat tttacggatt atgcatcata
     2341 atgttattac ttctcttgaa attctataat ccacaattag cagtcttaac tggagcaaaa
     2401 aattggtttt atattgctgg tttttcgttt caacctactg agttagtcaa aataggatac
     2461 acccttatgc tagctaaaat aaccgtcaat tatcagcaaa aatctattac tagtaatacg
     2521 aaagatgatt ggatttattt acttaaaact tttattgcta ctcttccaat aggaatatta
     2581 ttattttttc agaacgatct aggaacaatg ttagtcttca ttgttattta tatttttatt
     2641 ttgtttacgt ctaacataaa tataaaaata attgccccaa taatcgccat tggtgttgtt
     2701 ttatccttaa tatttttata tttggttata tcagatacag gaagagattt cttattaaaa
     2761 ataggatttc acgactatca gtttaaaaga atagactcat ggttgaatcc atttttagat
     2821 ccaaatggat ctagctatca gttagcaaat agtttaattg ctattggctc tggcggactt
     2881 tttaggtact ggttttaatg tatcaaacat tcacgttcct gttagagaat ccgatatgat
     2941 attttctgta attggagaaa attttggctt tataggaagt tgtcttgtaa tattcctata
     3001 cttttattta atttatcaaa tgtataaaac atgttttaaa agcaataact tatttttcac
     3061 ctatatcggt ataggcattg ttgcaatgat tttttttcat gtttttgaaa atataggtgc
     3121 aagtatcgga ttactacctc taactggtat tccactcccc tatattagtc aaggtggttc
     3181 tgccattctt agtaattttt tggggttggg atttatgttg tgttgtagta gatatactaa
     3241 cgaaaaaaaa ggaacacaaa aatgaattca aaataacact ttatgaatag aatgtctatt
     3301 tcaataaaaa ttatgatacc ttaaagttaa tcagtgaact actcaccact tagctaaacc
     3361 tgacggttct taacgcttga agtgggagct tcttgggaat agagcatact tgatagcgta
     3421 tttctttact aacagcggtg tctcttattt accaagctat ccccgtagtt cctacggttc
     3481 ttgatgattg gatctaagcc aatcccatag aatgtagtct tagaccttcg gtcagaatat
     3541 tgatactagc attgatatca cgatcatgat gagtatgaca aacaggacaa gtccattctc
     3601 gaatttcaag agatttcttg ccatctttat gtccgcactc tgaacaaatc tgactagacg
     3661 gaaaccattt atctactttg atgatttttc gtccatacca gtcagccttg tattgtaatt
     3721 tagtcacaaa ctttgaccaa gaaacatcag aaatactttt agctaattta tgatttcgca
     3781 acataccttt tgtgtttaag tcttcaatac agataatatc gtgatttttg atcatttctg
     3841 tactcaactt attcagaaaa tcagtacgtt gattcattac tttttcatgc aatctagcta
     3901 ctttgcgttt ttgtttttga taatttttag cttcaaatag attggtacct ttatttttag
     3961 ccaataaagc acgcctagac aatttgcgtt gttcacgctt tagtttcttt tccattttgg
     4021 acgtgaattt attattatca attttttgtc catcagaaag aatcgcaaag tccataatac
     4081 ctaagtcaat accaattgtg gaattagttt taggtaattc gataatttct tctttacaca
     4141 acaaagaaat atagtattta ccgctagaat gacgtgatat tgtagcagat ttgataatcc
     4201 cttttggttg tctatgaagc ttaattctta ctaatgactt caatttagga actttgatga
     4261 atttactatc aatcaaagca actgtaccat tttgattatt cgttgtataa ctctgaacag
     4321 gatttttctt acttttgaaa cgtggaaatc caacggattt atcccgaaaa aaattcttgt
     4381 atgctttatc taaattaagt tgggcattgg ctaaagcaag gctatcaatt tctttcaaaa
     4441 acggaaattc tttcttgtat ttagctggtg tcggaaaagt cattttttta gaagaatcat
     4501 ttttaacttc ttcatacgcc ttttttcggt catcaagcat tagattgtag accttacgga
     4561 cacaaccaaa agatttggca aagaagattt cttgttcctc tgttggatag attctgaatt
     4621 tgtatgcttt tagttgttcc ataaagttca cctactttct atttatgatt gtttccttga
     4681 ttttggatat acttcttaat cacatcaatt ggtgcgccac cagttgtcag aagacaaaaa
     4741 cttttagacc agaacatttc tttccaaaga aattgtttga ctcttggaaa gtcacgtttt
     4801 atcaatcttg agctggcact tttataggca ttgatgaatt ttgtcaattc tgttttaggt
     4861 tgagctttga acataatatg aacgtggtct ttatcatgat tccactccac taaagtaatg
     4921 tgatacgatt ctgaaattct ttcaaaagta gttttggcat aatcagatat ttcatcatca
     4981 atcacttgtc tacgatattt cgttactaaa acaaggtggt aataaagaag aaatactgaa
     5041 tgattatttg tatctaattc catgttatat caactcattt cttgtataga ctgattgtaa
     5101 catggaacaa aataaaaagg caatacgcct tagttgtcgg tcttcgagtt accaacggta
     5161 accctcatac cgaacggcat tcatcaccca cctatagagg atgggcgact tctgcctaat
     5221 tctgttaaat ttagtaacaa gttttgtcat tataccagta attataggaa tgtctattta
     5281 tttcatcaaa aaaaataaac aatttttttg aatgaaatta cgggcgaaaa taatcactca
     5341 aaattattaa taaaaacgaa tcaactactt tttctgaaga aacatacaac cgtaaacaac
     5401 aattgttgtt tacggttgta aattatcaaa ctatatggtg acttgttgct taaaataggt
     5461 tgaaatttcg tttatttttt tgagaaattt tacccacgta ctacccatgt acctatccac
     5521 gtattaccca tgtacccccc catgacaaat gggcggaggg gcttgcggcg cttggtatta
     5581 aaggcattat ccgcctccca ggtttctgac cattttctct cataagtggg gtttttaccg
     5641 cgttgagaga aaatggtcag aaacctaaac gatcggcaaa gaaaacatac caagcgccac
     5701 aagcaaaaaa agttagagta acttccagaa aagaaaaatt tttagaacaa cttttgaaaa
     5761 tttgattttg atttatatcc gttcaattta aacatttgat aaactgaagg ctagtattta
     5821 aaatgtgtat acaagtggta gcgttaaaaa aagtggaggt ataacctatg ttaaaaaaaa
     5881 ctgtgttggt actgggttct atttctctag gaatattagg ttttagcccc atgctattat
     5941 ccgtaattgg aaaaaacaat tccaacagag aacttaaatt aaattcaaat tattacaaca
     6001 gtcctaacga agaattgttt gtaaaacaaa cggataaata ataaaaaaga ctgctctttc
     6061 gagcagccag ttaaaaaata atgagtgttt attcttgaaa aagcacgcgc ttatgaagcg
     6121 ctaccaaaaa tttaccatat tttacaaaaa ataacaagtc ttaaaaaaat gacagataaa
     6181 aatgcacaaa gaattagata tagataaccg cgaaaaatca agctttagaa agttgctttt
     6241 agctagacca attaaaaatc acaaagtgag cgtctctatc cattggtaga ggggctactt
     6301 ggcatagtgt acattatgtt aactaacgct ataaataacg attatgttcc caaaacaata
     6361 ttttatgaac tataacaaaa atttgttatc gcatattgta tattttaatt ataggtgtat
     6421 accacttaaa tttaacacaa aggcgtgatt attttgaaac ccaaaaagaa aatttatcta
     6481 ggtctcctct tttccttgat gattggaatt tttagtatag gaattacaaa tacctatgct
     6541 tcagaaaatt ctaatttcaa tatcaactcc caaccaattg aagaacaagc aattaatttt
     6601 gcgaaaactg attttgaaca ctccataact tcaattcttt taatggaaaa taacaccgaa
     6661 actgatatta actatactct tggttcgcca tttaaaatgc tcgatggaga aaatacggca
     6721 aatagttcta ttttctttcc agtattttca gacgctgata atacgattaa atatatttat
     6781 accgtcaaaa aaaatacaaa tggagaatat tcaggaagta tttcacaatt tttagcaaaa
     6841 gaacttcaaa atctagtaga tagtaatata acagatacaa ccttattttt tcaagataat
     6901 agcatttatt attccgataa tggacaaatt aaactacttt ggaaatcacc tttaagtaca
     6961 gaaaaaaatg gtgtttcttc ctttacagaa aaaaatgatc ttatatccgt aaatataaaa
     7021 aataaaatta aaaatttctc taaaaaacaa aattttcctg cgattaaacg tgtaaaaagt
     7081 gtctcctccg gagataactt tattatgatt aattggcgta tagcagaaac ccaaacaagt
     7141 tttccgtggt gtgctgctta cgtagaagct gctatcttat gtaacaaaac agatgtacat
     7201 gctacttcag ctagagccat tatgcattat acctatccta atttaaacaa cagtcaaatt
     7261 gaatcaaaaa gtcttaatca agatcaaata ataaaatatg caaattactg tggtgtttat
     7321 cctaaaaaag tagcacgaat actaaacctt tcagaggtcc aaacacaact aagaaaagga
     7381 aatgccattt atatgggaac tgctggttat ggaaattata gtaacagccg tcatgcactt
     7441 gcattatttg ggtgggtcaa tcaaggtggt tatcaaactt actatgtgtg gaatccttgg
     7501 tataactacc caactgtaat aagtgcaaat gcaggaccag ttacaattcc tgtacctgga
     7561 ggtggtttta cttgggacaa taccattaca aattggtaaa taaacccatt gctttagtta
     7621 tgggtctagt gttagttctt ttcttaacta gttgttcaaa aaaacaagaa atacaaaaga
     7681 ctgatctgat tccttttcta ggaacttggc aaactaaaga tcattcagaa cgtctagtaa
     7741 tcactaattt aaatacttca aaaaatatag aacaatatac catcttagta aaagagaaca
     7801 atcataaaat tcaaatcatt gataaaaatt taaatataaa tgaacaagtt ttcatttaca
     7861 aagcaacaaa taatttacta acactgcgct ctaataaaaa acaattagat tacactttta
     7921 gtttggattc tccaaaaaag ctcgtattta ttttgacaac gaatgatggg acagaaggga
     7981 cagctaaacc aattgaattt tttaaagctg attgatgaat agttgtttga tattacaaaa
     8041 caataatttc accgaaggtg aaacaagcga aactttacaa aaagtggcta gcatttgcta
     8101 gccttttttg tattgtggag cgtgggtttt gagggggatt caagggggag cttgcgcccc
     8161 tttgaaaagc ggttatttgc accaaatacg taggtatttg gctagcaaat atagagcttg
     8221 ccaaaccctt ttagtgagct ttactcattg taccttttta ggtacaatgg tattgcgaac
     8281 gatacgtccg taggacgtac tgaaagggtg ggacttatga gcgaacaaaa tcaaaatttg
     8341 gctagcgatc attctaaaaa atatacctat cgatccgagc caaaacaaat cagttttcga
     8401 gtgagcgaat ctgaatttgc aaagctgaag cagtcagctg aagctttgca aatgagtgtg
     8461 ccggctttcg taaaagccaa ggcacaaaac gcgcgtctgg tgacaccgaa agtggcgcca
     8521 gacattgccc aggcaatcgc gcgcgattta gccaaagctg gagggaatat caatcaaatt
     8581 gctaagtggt gtaatacaca ccaacacgac gttgctcctg gagacgccca acgcttatcc
     8641 gaaaacctaa aaatcatgca aaaggagcta caaaagatat ggcaacagtt aaagtaagcc
     8701 gaaccacttc ttgtagtcgt gcaattaatt acgctgaacc acgagcaacc attaaaacag
     8761 gaataaactg tgacgtcgat tatgccaaaa gtgaaatgaa acaaattcgc atgctgtatg
     8821 gaaaagacga ccgtgtacag gcacacttat tgattcaatc ttttcgacca ggagaaatta
     8881 ccgctgaaaa agctaatcaa ctaggggcag agtatgcaaa aaaaatagcc cccgatcacc
     8941 aaatagctat ttacacacat acagacaaag accatattca taatcacatt gtcattaact
     9001 ccgttaatct ggagactgga aaaaaatttc aagctcatgg tcaagcattt ctaaacaaat
     9061 gttatgacct aaatgacgag atttgtcttg cacataactt accaatcact gagcgaggaa
     9121 agaaaccaga aaaacgaacc atgtctgaaa taaaattaaa agaaaaaaat gaacctgttt
     9181 ggaaagacga aattcggatt gcgatcgatc aaacaatgaa aaacaaaaaa accagaacct
     9241 atgaccaatt ttgtaattcc ctcaaaacat tggggattca ttgcttcaat cgcgggaaaa
     9301 atttcactta cgaattgatc cacaaaaaga aaaaagtacg atccaacaga ctaggaaagg
     9361 actacgaaaa ggagacgatt ttacatgagc tggacagacg agaacgaacc tataaccaac
     9421 agcgaaccaa cgagctacga agagctcgag ctagcattcg cgaaactgca gaagaagtac
     9481 gaagagaaca acgaactctt acgcctgaac aaccggaaat tagaccggct cgaagaaacc
     9541 atgaacaaca cgttgaaaga ggaacagacc accaacaaga attatcgctc taaaacgtta
     9601 aaagacatag aaaacgaaat aaagaaacaa cgtgcttttc tcgttcctta tacgcaagaa
     9661 aggatacaaa ctacgattga tttacaagtt aaaaaactcc atcaagagct attagaccag
     9721 cgaaataagg aacagaaaaa ggtgcttatt caacgatcat tggattttct tgccccctta
     9781 tgttgcttag tcctgacact gttcgcattt tggttagtat ggggaggcta aaatggcatt
     9841 ttatttaccc tatctactca tttttgttag tatttcagga agtatttggc tcatctataa
     9901 aatttttcaa acaagatatt cattaaaagg ctctaaaatt cgatttaagc gatttttctt
     9961 tcttggctgt atattttcct tgataatcgt ttcaagcggc ttattcggcg ttttagaggg
    10021 aaataagcgc gtttctagaa gtattttatt aggaaatgtc actcaaaagt atgaatcagc
    10081 gcgtgataaa aagaaaaaag agcaagtatt agcacaaaaa atagaaaaat ttaccgcttg
    10141 ttatgaggac atgaatgata tttttgtgaa gcaagaaaag cgtttgacgg ataaaaatat
    10201 ggaaacactc actcgacttt atcggaattt gccagaagaa cagcaaaaag agtaccaaga
    10261 aaaatatgaa caagtaaaaa aagacgtgca atatgtgaaa gatacgaaag tggaagaagc
    10321 ttgtagtgat ttatttggag atacaattcc tttttcaact agcgaacaag aacgaaaaga
    10381 acgacaacaa tcagtcacat atgaacgcta tgaaatcttg cttcaacaag caattaacat
    10441 acaaaatctg actaaaaaag aaacagcttt aaattattta aaaagcgtca aggaatggtt
    10501 agatcagcaa cagcaaaatt gaatttgatt gaaattcatg ttaaactaaa catgcaaatg
    10561 ttcaaacatt agatacacga aagccccaga gaggtcggat tctctggggc tttttttgta
    10621 ggttgagtcg cctttactga gttttacttc ttgtttcggt tattcaacca ataactgaat
    10681 aaagcgctta aaagcccaac aagaagggag caaataaagt tagtgaacac gaaacacacc
    10741 tccttccagt cgcaagactg tcggataggc gacaagttat tataccctac aaaattaaat
    10801 ctaattttaa gtttcttatt tccgggttgt tagggcttgc cctgaccgcc tgtagggcgc
    10861 tttaaatgcc agataaaggt ttttatacta agactgtctt aaaacggctt agacaagctc
    10921 ttagagcctc aaccctcctt tttgaacaat ggttttagca aaggagctgg aggcgactgc
    10981 gcaggtataa tgtgagagcg tagcgatcac acctttttgg cgaccgtagg gagcgaggtt
    11041 ttgaattagc agtttagaaa agttcgcgaa cctgtttaaa gaaaaaataa ttgttttttt
    11101 aggtgggaaa ttttgtctcc ctcttttata atcttttttt aaacctcttt ttaaacctct
    11161 ttaccgtcgg ctgtgtcctt actcccccaa gggatacaaa attttatagg catgaaatta
    11221 tctgtttata ggcatgaaat tatctgttta taggcatgaa attatctgtt tataggcatg
    11281 aaattatctg ttttaggtat tgtttttaga cataatatgt cctaaaatga tgtcaataaa
    11341 atcatttaga ctggagtaac aactaa
//
