LOCUS       CP063592                4305 bp    DNA     circular BCT 04-APR-2022
DEFINITION  Enterococcus faecium strain BMT-2-1-1 plasmid pSS-1-1_06, complete
            sequence.
ACCESSION   CP063592
VERSION     CP063592.1
DBLINK      BioProject: PRJNA669626
            BioSample: SAMN16483159
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Both,A., Kruse,F., Mirwald,N., Franke,G., Christner,M., Huang,J.,
            Hansen,J.L., Kroger,N., Berneking,L., Lellek,H., Aepfelbacher,M. and
            Rohde,H.
  TITLE     Population dynamics in colonizing vancomycin-resistant Enterococcus
            faecium isolated from immunosuppressed patients
  JOURNAL   J Glob Antimicrob Resist 28, 267-273 (2022)
   PUBMED   35134550
REFERENCE   2  (bases 1 to 4305)
  AUTHORS   Both,A., Kruse,F., Christner,M., Huang,J., Mirwald,N., Franke,G. and
            Rohde,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) Institute of Medical Microbiology, Virology
            and Hygiene, University Medical Center Hamburg-Eppendorf,
            Martinistr. 52, Hamburg, Hamburg 20246, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Trycycler v. v0.3.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 705.1x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/27/2020 16:53:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.13
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 3,270
            CDSs (total)                      :: 3,179
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 59 of 128
            Pseudo Genes (incomplete)         :: 65 of 128
            Pseudo Genes (internal stop)      :: 16 of 128
            Pseudo Genes (multiple problems)  :: 11 of 128
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="BMT-2-1-1"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pSS-1-1_06"
                     /geo_loc_name="Germany: Hamburg"
                     /lat_lon="53.33 N 10.1 E"
                     /collection_date="2014-07-08"
                     /collected_by="Institute of Medical Microbiology, Virology
                     and Hygiene, University Medical Center Hamburg-Eppendorf"
     gene            complement(42..1448)
                     /locus_tag="IQN11_16330"
     CDS             complement(42..1448)
                     /locus_tag="IQN11_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="UOC39587.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDENI
                     YLEQINIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKMEPQREFIVQIGTL
                     DDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPSLSVYNAVIHNDEVSP
                     HLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIERLMA
                     DFGWERELVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYICRQREENEREK
                     ALVASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDFVDLYQR
                     ATRATNAKRNADNMSEQYLQMLEQNTELNEYVAALEEERHLSGRKQLADLQVENKKLKE
                     ENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFYRQNVSQHK
                     LERSEDEIEW"
                     /name="plasmid recombination protein"
     gene            complement(1670..2032)
                     /locus_tag="IQN11_16335"
     CDS             complement(1670..2032)
                     /locus_tag="IQN11_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOC39585.1"
                     /translation="MGMTLADIKVQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKRF
                     GFESFADFERWLSSISDDQQHEKSVGNELRADEKSAPSERNYPNESDGIFGGIPNRKSQ
                     MSESGFASMFSDVNSR"
                     /name="hypothetical protein"
     gene            complement(2036..2374)
                     /locus_tag="IQN11_16340"
     CDS             complement(2036..2374)
                     /locus_tag="IQN11_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOC39586.1"
                     /translation="MVAILEVRCDMDKIDLREETVNRIEVMYERLADYLNLSDEDNYSE
                     MLERMMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDEAEKEFWLKLMSHIMQLNQISV
                     INSKEKGM"
                     /name="hypothetical protein"
     gene            complement(2353..2772)
                     /locus_tag="IQN11_16345"
     CDS             complement(2353..2772)
                     /locus_tag="IQN11_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOC39583.1"
                     /translation="MTDSTDKMTIKALADELGITKNTVRNYLVKAGYDLARYKKNGIIL
                     LDKELIGVARSIYRSKGGQLTGHLPVNSTGSDFPLEKENEFLRDQLAEKDKHISELTEM
                     IRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
                     /name="hypothetical protein"
     gene            complement(2769..3758)
                     /locus_tag="IQN11_16350"
     CDS             complement(2769..3758)
                     /locus_tag="IQN11_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="UOC39584.1"
                     /translation="MKKKKQDLINGTFREKKKNLKKVVAFSDNRISPKMRELMTGCGSY
                     LEFIATIDKEKKKLVQAHFCKNRFCPLCAWRKARKDSMMLSIMMQAIAEEKQYQFIFMT
                     LTTPNVKAECLNDEIDLFNHALKKMFMRKRVKTSIKGYVRKLEITYNKKRNDYNPHFHL
                     ILAVNKSYFTDKDYYIKQSEWLDLWREVTGKTGINPDGTDEITQLDIRKVKGFQQEKAV
                     QEVAKYSAKDFELTESQEVFDTFYHAMKGRQLITFNGVFKEYKKKYESGDLDRYKKKDE
                     NEYFWFLTASWMRKESKYAVDYREMSADERNYFSGMYVDEKSVEGENE"
                     /name="protein rep"
     Typing          complement(46..1448)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.359"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cggcacaaac cgcctccggt accgttgact gcacatcgta actaccactc gatctcatct
       61 tcagaacgct ctaatttatg ctgtgagacg ttttgacgat aaaataaggt tttctcctgc
      121 tctaatactt ctaattcctc agaaagcccc atatcctttt ctgcaagccc aaaacggata
      181 gacaaacgag tgatcgattt ttgaaatgcg ttttttaatc gctctaaacg ctggcgtaaa
      241 gagcgatttt cttctttcag ctttttattt tccacttgta aatctgctaa ttgttttcta
      301 ccagacaaat gacgctcttc ttccaatgca gctacatact catttaattc tgtattttgc
      361 tcaagcatct gtaaatattg ctcactcatg ttatcagcat ttctcttggc atttgttgct
      421 ctcgtagctc gttgatacaa atcaacaaaa tcattcccag ataaatgata cgaaccatca
      481 ggaagcctct tgaaactttc taaaggaacc atatcttttt ggaaaatgac cgccatctct
      541 ttttttacat tcagcatatt tttcttctct tcttcaatct tcgctctctc agaagccacc
      601 agagccttct cacgctcgtt ttcttctctt tgcctacaaa tatacctcaa ctcatcagaa
      661 acgctctcac gctcttttac ggccgtttga cgcaattcgg aaatttcgct catgatttct
      721 ttgtattcat gaatatcttt gattttgtta gtaccgacta actcacgctc ccaaccaaaa
      781 tctgccatca aacgttcaat tgactcaact tcctgattcc taaaattatt ccaaatcact
      841 cgaccattct ctttatctgc tcgtaaaccc tgctgttgca aggctttgtt aaaacttggt
      901 tgcttttgaa caccccgttt atacccttca gcaacaggaa caatattcaa atgcaggtgc
      961 ggacttactt cgtcattatg aataacagca ttgtagacgg ataaactagg attccgttct
     1021 ttaaattctt tgaaatactc aactaaaatt ttgttggcaa tcactcgatt ttgttctgct
     1081 tgttgttccg atatgccagt agaagaacca tcatcacgag tagtacgaaa atcatcaaga
     1141 gtaccaattt gcacgataaa ttctcgctgt ggttccattt ttttatcctt gcgaaccttt
     1201 gctaaatagt cctcaatttt tctatcggct cttttctgct ttgcattata tttttctacg
     1261 gcttcaccga ataattcttc atatttttca cgaatattta tttgttccaa atagatattt
     1321 tcatccgatt ttgtgaaatc aatatgctta tgccagtcat tgattttttg gctatcagtt
     1381 aactcacgat tattatgaga aatgcttgta ttctttgttc cctttttgaa cgaaatacta
     1441 aaccccaagt taaacccctc cttcgcgctg tcgctaataa aaagagtgta aacctttctg
     1501 ttactctttg tcaaagagta acgttttcct aattgtgaca tgactgtcac aattccaaac
     1561 gctctccgag ggttttcgcc gtcacaagtg acaacgaaaa aaggagccga aagcgtgtca
     1621 cgctctcaac tccttttacg ctttattttg cttcatgcaa aatattcgct tatcgtgaat
     1681 ttacgtcact aaacattgac gcaaatccac tctcactcat ctgacttttt ctattcggta
     1741 tccctccgaa tattccgtca gattcgttag ggtaattccg ttcgcttggt gctgattttt
     1801 catcagctcg caactcatta cccactgatt tttcgtgttg ctgatcgtca gaaatcgacg
     1861 atagccaccg ctcaaaatca gcaaaagatt caaacccaaa acgcttgcga agagccttgc
     1921 ccattgcagc attttctttc tcaatcaatc gtttcttctg ttggcgaagt ttcgcctgtt
     1981 ctttttttaa tttttcttct tgcactttaa tatctgctaa agtcattccc ataaactaca
     2041 tccccttttc tttggaatta ataacggaaa tttggtttaa ctgcataata tgactcatta
     2101 atttcaacca aaattctttt tcagcttcat cagttgtttg cgctaaatgc ttatgaacaa
     2161 attcattagt caaaactgaa gattttgcta gatcagcatt gataaccgag ttgagaacat
     2221 ccatcattcg ttcaagcatc tcactataat tatcttcatc cgaaagattc aaataatcag
     2281 ctaaccgttc atacatcact tcaattctat tgactgtttc ttctctcaaa tcaattttat
     2341 ccatgtcaca tctcacttcc agaattgcca ccattttcga ttttttaatt cttgattttc
     2401 tagtaacaac tgatcgtttt tttgttgctg aagcagggtc aatttttgtt gatcacgaat
     2461 catttctgtt aattcagaaa tatgtttgtc cttctcagct aattgatcac gcaaaaactc
     2521 attctctttt tccaaaggaa aatcactgcc cgtgctattg accggtaaat gaccagtcaa
     2581 ttgaccacct tttgaccgat aaattgaccg agcaacacca atcaactctt tgtctagcaa
     2641 gataattcca tttttcttgt accgagcaag atcatatccg gctttgacta gataatttct
     2701 cactgtattt ttggttattc ctagttcatc agcaagcgcc ttaattgtca ttttatccgt
     2761 tgaatcagtc attcattttc cccctcaaca gatttttcat cgacatacat tcctgaaaaa
     2821 taatttcttt catcagcact catctcacga taatcaaccg catatttcga ttctttcctc
     2881 atccacgaag cagtcaaaaa ccaaaaatat tcattctcat cttttttctt ataacgatca
     2941 aggtcacccg actcatattt tttcttgtat tctttgaata caccgttaaa cgtaatcaac
     3001 tgacgacctt tcattgcatg atagaatgtg tcaaacactt cctgactttc ggtcagttca
     3061 aaatcttttg ccgaatattt cgcaacttcc tgtactgctt tttcttgttg gaaccctttg
     3121 accttgcgaa tatccaactg ggtaatttcg tcggtaccat ccggattaat tcccgttttt
     3181 ccggtcactt cccgccacaa atctaaccat tccgattgct tgatataata atctttatcc
     3241 gtgaaataag atttatttac tgccaaaatc aagtgaaaat gcgggttata gtcattccgt
     3301 tttttgttgt acgtgatttc caattttcgc acatagcctt taatcgaagt cttaacccgt
     3361 ttgcgcataa acatcttttt caatgcatga ttaaacaaat caatttcatc gttcaaacat
     3421 tccgctttta cattcggtgt cgtcaacgtc ataaaaataa attgatactg cttttcttcc
     3481 gcaatagctt gcatcataat cgacaacatc atcgaatcct tacgagcttt acgccaagca
     3541 cataacggac agaaacgatt tttacaaaag tgagcttgga ccaatttctt tttctcctta
     3601 tcaatcgttg caataaattc taaatatgaa ccacagcctg tcatcaattc acgcattttg
     3661 ggagaaattc gattatcact aaatgccacc acttttttca aatttttctt tttttctcga
     3721 aatgttccgt taatcaaatc ttgctttttc tttttcatct tgctatactg aatctacaaa
     3781 ttttgtatac aaaaaaggct gaaaagccga taacaaaaaa tagattgctc tcctttctag
     3841 tttagttttt ttctagtcaa ttaaaactat aaaatactag ggaagaaaaa gcaatctttt
     3901 tttttgttct tccttagagc cacaagggat agacccatac tatagccccg atttcttcgc
     3961 agaaatagtt gcctatagta tcaagataag aaaaaacatc aaaaaagagg gcgaaaatcg
     4021 cccccttttt ttcgttttaa aaccccaaaa cccaaagaca taaaattcga tcaatcgacc
     4081 actgcatttt taagggggtg tacatatttt gtacatttaa ccgcctgtta gatagggctg
     4141 ttaaattttc ccactcattc ttagtcatgc aaacataaaa ccgcaccctt taaaaagggc
     4201 gcccccaaac ttcgctaacg ctaagttggc caaaaaatgg acttgccatt ttttagcaaa
     4261 tgtcagctta ataggctagt ggtttagccg tcaactcctt atcct
//
