LOCUS       CP063591                7166 bp    DNA     circular BCT 04-APR-2022
DEFINITION  Enterococcus faecium strain BMT-2-1-1 plasmid pSS-1-1_05, complete
            sequence.
ACCESSION   CP063591
VERSION     CP063591.1
DBLINK      BioProject: PRJNA669626
            BioSample: SAMN16483159
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7166)
  AUTHORS   Both,A., Kruse,F., Mirwald,N., Franke,G., Christner,M., Huang,J.,
            Hansen,J.L., Kroger,N., Berneking,L., Lellek,H., Aepfelbacher,M. and
            Rohde,H.
  TITLE     Population dynamics in colonizing vancomycin-resistant Enterococcus
            faecium isolated from immunosuppressed patients
  JOURNAL   J Glob Antimicrob Resist 28, 267-273 (2022)
   PUBMED   35134550
REFERENCE   2  (bases 1 to 7166)
  AUTHORS   Both,A., Kruse,F., Christner,M., Huang,J., Mirwald,N., Franke,G. and
            Rohde,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) Institute of Medical Microbiology, Virology
            and Hygiene, University Medical Center Hamburg-Eppendorf,
            Martinistr. 52, Hamburg, Hamburg 20246, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Trycycler v. v0.3.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 705.1x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/27/2020 16:53:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.13
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 3,270
            CDSs (total)                      :: 3,179
            Genes (coding)                    :: 3,051
            CDSs (with protein)               :: 3,051
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 59 of 128
            Pseudo Genes (incomplete)         :: 65 of 128
            Pseudo Genes (internal stop)      :: 16 of 128
            Pseudo Genes (multiple problems)  :: 11 of 128
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="BMT-2-1-1"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pSS-1-1_05"
                     /geo_loc_name="Germany: Hamburg"
                     /lat_lon="53.33 N 10.1 E"
                     /collection_date="2014-07-08"
                     /collected_by="Institute of Medical Microbiology, Virology
                     and Hygiene, University Medical Center Hamburg-Eppendorf"
     gene            1..738
                     /locus_tag="IQN11_16285"
     CDS             1..738
                     /locus_tag="IQN11_16285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UOC39578.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEIF
                     TFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDKVN
                     ETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKGHWF
                     VKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAYKGNK
                     IGSLQIYFEENIPSITLDNWLEK"
                     /name="replication initiation protein"
     gene            767..1288
                     /locus_tag="IQN11_16290"
     CDS             767..1288
                     /locus_tag="IQN11_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="UOC39579.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKVV
                     LRSGVLQLASNLGNQRLLDRLHEYEQENDKNVYISRKNVVVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEEPEEEVLTSVNKENNNAI
                     TTEKKWWKFW"
                     /name="DUF536 domain-containing protein"
     gene            1372..1676
                     /locus_tag="IQN11_16295"
                     /pseudo=""
     CDS             1372..1676
                     /locus_tag="IQN11_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /name="helix-turn-helix transcriptional regulator"
     gene            2235..3389
                     /locus_tag="IQN11_16300"
     CDS             2235..3389
                     /locus_tag="IQN11_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="UOC39580.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQPI
                     EEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDA
                     DNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIK
                     LLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDNFIMI
                     NWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKSLNQDQ
                     IIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQ
                     GGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
                     /name="peptidase C47"
     gene            3383..3805
                     /locus_tag="IQN11_16305"
     CDS             3383..3805
                     /locus_tag="IQN11_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOC39581.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDHS
                     ERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNKKQ
                     LDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
                     /name="hypothetical protein"
     gene            4109..4489
                     /locus_tag="IQN11_16310"
     CDS             4109..4489
                     /locus_tag="IQN11_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UOC39575.1"
                     /translation="MSEQNQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4471..5385
                     /locus_tag="IQN11_16315"
     CDS             4471..5385
                     /locus_tag="IQN11_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="UOC39576.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5625..6314
                     /locus_tag="IQN11_16320"
     CDS             5625..6314
                     /locus_tag="IQN11_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOC39577.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTTCY
                     KEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYENLFQQATNMQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(6436..6522)
                     /locus_tag="IQN11_16325"
     CDS             complement(6436..6522)
                     /locus_tag="IQN11_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017548328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="UOC39582.1"
                     /translation="MFEQFICPLLVGVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     Typing          2..738
                     /name="rep_cluster_1742"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4472..5382
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttgagtaacg atatagttcg atataataat ggattaaatt ctgtcccttt aagaaaattt
       61 acccctatag atatggattt attttgggga atatgttcta aaatgaaaag aaaaggtaca
      121 aaagaagaaa tttttacatt taaacaaata aaagaattaa ttgattacaa tataaatgca
      181 cgtggcgacc gttttgctaa tgatttaata aaaatgtcaa aaaaaatcat tactttgtct
      241 tttatttatg aagacgaaaa aacatatgaa caattaaatc tgtttcaaaa atttaaaatc
      301 gataaagtga atgaaacatt attaataaag gtttctgaag aatttgaata tatacttaat
      361 tcaatcggaa ctaattttac tcgtttcgaa ttagaaaata tgacaaactt aaagagtagc
      421 tacgttaaag agttatatcg ccaacttatg gctcataaag acagaagatc taaaaaaggt
      481 cactggtttg taaaaataga agattttaga gctctattag caataccaga aagttatcgc
      541 atgagagata ttgataaaca aatttttgcc caagcgaaaa aagaattttt aaacgttaat
      601 tcaactcacc ctgctatttt taaaactttt gaaatcaaaa aaattaaagc ctacaaagga
      661 aataagatag gatctctgca aatttatttt gaagaaaata taccttcaat taccttagat
      721 aattggttag agaaataata acctaacaat ttaggaggca attatcatgc aacccaaaga
      781 aacctttaca ataaaggaat tatctgaatt atttgaaata agtaggcaag caatttctaa
      841 acatgttgat aaattagacc cgtccttatt agctaaaaat gagcgtggtt ataaggttgt
      901 attacgttct ggagtattgc aacttgctag caacttaggt aaccaaaggt tactagatag
      961 gttgcatgag tatgaacaag aaaacgataa aaacgtttat atatcaagaa aaaacgtggt
     1021 tgtaaacgag ttgctagata agttgcgtga agaaaatgaa tttttacgag aacaactttc
     1081 tctcaaagag caaaataatt ctaatatgca aaaattactc gaccagcaac aacaactcac
     1141 cttgcaggca aatcaacaga tcgaaaaact gcaagaacag ttacaattag cttatacgga
     1201 agaacctgaa gaagaggtat taacttctgt caataaagaa aacaacaatg caataacaac
     1261 agaaaaaaag tggtggaaat tttggtaaca attaccaact ttccataaag aagaaatcaa
     1321 tttctataga ctttatcttt ctaaaaataa aaaatatgtt taggtgatca aatgactata
     1381 agtaaagtaa tcaaacaaaa aagaatagaa aaacaactaa ctcaagaaga tttagctgaa
     1441 atgcttttag tatctaaaaa aaccatttct aattgggaaa atggtagaac aataccagat
     1501 acagaaaatt taattcaatt tgctaagtta tttgatttat cactagacaa tattttgtta
     1561 ggagaagaaa aagttatgtt aaaaaatcat acaaaaacac taatactatt atttcatttt
     1621 gtaatttttt taagtttagt ttttacctta attcagtcca aattctttta ttttagccat
     1681 tcaattgcca cttggttatt aatatttttt ctaagtttgc tattagtaat caatactctg
     1741 attattattt ttaataaaca taaaaattta tttcttcata gtgtttttcc aattattttg
     1801 ctgagttttt tatactttgt ataaagtata aaaaaagact gctctttcga gcagccagtt
     1861 aaaaaaataa tgagagttta ttcttgaaaa agcaacgcgc ttataaagcg ctaccaaaat
     1921 tttatcatat tttataaaaa ataacaatcc agtaaaaaat gactggtaaa aattcacaaa
     1981 gaaacggaaa gaagcaactg cagaaaatca agccttaaaa aaagcttttg acctagacca
     2041 atgaaaaatc acaaagtgag cagctatagc ccttgataga ggggctactt ggcatagtcg
     2101 acattatgtt aactaacgct ataaataacg attatgttcc caaaacaata ttttatgaac
     2161 tataacaaaa atttgttatc gcatattgta tattttaatt ataggtgtat accacttaaa
     2221 tttaacacaa aggcgtgatt attttgaaac ccaaaaagaa aatttatcta ggtctcctct
     2281 tttccttgat gattggaatt tttagtatag gaattacaaa tacctatgct tcagaaaatt
     2341 ctaatttcaa tatcaactcc caaccaattg aagaacaagc aattaatttt gcgaaaactg
     2401 attttgaaca ctccataact tcaattcttt taatggaaaa taacaccgaa actgatatta
     2461 actatactct tggttcgcca tttaaaatgc tcgatggaga aaatacggca aatagttcta
     2521 ttttctttcc agtattttca gacgctgata atacgattaa atatatttat accgtcaaaa
     2581 aaaatacaaa tggagaatat tcaggaagta tttcacaatt tttagcaaaa gaacttcaaa
     2641 atctagtaga tagtaatata acagatacaa ccttattttt tcaagataat agcatttatt
     2701 attccgataa tggacaaatt aaactacttt ggaaatcacc tttaagtaca gaaaaaaatg
     2761 gtgtttcttc ctttacagaa aaaaatgatc ttatatccgt aaatataaaa aataaaatta
     2821 aaaatttctc taaaaaacaa aattttcctg cggttaaacg tgtaaaaagt gtctcctccg
     2881 gagataactt tattatgatt aattggcgta tagcagaaac ccaaacaagt tttccgtggt
     2941 gtgctgctta cgtagaagct gctatcttat gtaacaaaac agatgtacgt gctacttcag
     3001 ctagagccat tatgcattat acctatccta atttaaacaa cagtcaaatt gaatcaaaaa
     3061 gtcttaatca agatcaaata ataaaatatg caaattactg tggtgtttat cctaaaaaag
     3121 tagcacgaat actaaacctt tcagaggtcc aaacacaact aagaaaagga aatgccattt
     3181 atatgggaac tgctggttat ggaaattata gtaacagccg tcatgcactt gcattatttg
     3241 ggtgggtcaa tcaaggtggt tatcaaactt actatgtgtg gaatccttgg tataactacc
     3301 caactgtaat aagtgcaaat gcaggaccag ttacaattcc tgtacctgga ggtggtttta
     3361 cttgggacaa taccattaca aattggtaaa taaacccatt gctttagtta tgggtctagt
     3421 gttagttctt ttcttaacta gttgttcaaa aaaacaagaa atacaaaaga ctgatctgat
     3481 tccttttcta ggaacttggc aaactaaaga tcattcagaa cgtctagtaa tcactaattt
     3541 aaatacttca aaaaatatag aacaatatac catcttagta aaagagaaca atcataaaat
     3601 tcaaatcatt gataaaaatt taaatataaa tgaacaagtt ttcatttaca aagcaacaaa
     3661 taatttacta acactgcgct ctaataaaaa acaattagat tacactttta gtttggattc
     3721 tccaaaaaag ctcgtattta ttttgacaac gaatgatggg acagaaggga cagctaaacc
     3781 aattgaattt tttaaagctg attgatgaat agttgtttga tattacaaaa caataatttc
     3841 accgaaggtg aaacaagcga aactttacaa aaagtggcta gcatttgcta gccttttttg
     3901 tattgtggag cgtgggtttt gagggggatt caagggggag cttgcgcccc tttgaaaagc
     3961 ggttatttgc tagccaaata cgtaggtatt tggctagcaa atatagagct tgccaaaccc
     4021 ttttagtgag ctttactcat tgtacctttt taggtacaat ggtattgcga acgatacgtc
     4081 cgtaggacgt actgaaaggg tgggacttat gagcgaacaa aatcaaaatt tggctagcga
     4141 tcattctaaa aaatatacct atcgatccga gccaaaacaa atcagttttc gagtgagcga
     4201 atctgaattt gcaaagctga agcagtcagc tgaagctttg caaatgagtg tgccggcttt
     4261 cgtaaaagcc aaggcacaaa acgcgcgtct ggtgacaccg aaagtggcgc cagacattgc
     4321 ccaggcaatc gcgcgcgatt tagccaaagc tggagggaat atcaatcaaa ttgctaagtg
     4381 gtgtaataca caccaacacg acgttgctcc tggagacgcc caacgcttat ccgaaaacct
     4441 aaaaatcatg caaaaggagc tacaaaagat atggcaacag ttaaagtaag ccgaaccact
     4501 tcttgtagtc gtgcaattaa ttacgctgaa ccacgagcaa ccattaaaac aggaataaac
     4561 tgtgacgtcg attatgccaa aagtgaaatg aaacaaattc gcatgctgta tggaaaagac
     4621 gaccgtgtac aggcacactt attgattcaa tcttttcgac caggagaaat taccgctgaa
     4681 aaagctaatc aactaggggc agagtatgca aaaaaaatag cccccgatca ccaaatagct
     4741 atttacacac atacagacaa agaccatatt cataatcaca ttgtcattaa ctccgttaat
     4801 ctggagactg gaaaaaaatt tcaagctcat ggtcaagcat ttctaaacaa atgttatgac
     4861 ctaaatgacg agatttgtct tgcacataac ttaccaatca ctgagcgagg aaagaaacca
     4921 gaaaaacgaa ccatgtctga aataaaatta aaagaaaaaa atgaacctgt ttggaaagac
     4981 gaaattcgga ttgcgatcga tcaaacaatg aaaaacaaaa aaaccagaac ctatgaccaa
     5041 ttttgtaatt ccctcaaaac attggggatt cattgcttca atcgcgggaa aaatttcact
     5101 tacgaattga tccacaaaaa gaaaaaagta cgatccaaca gactaggaaa ggactacgaa
     5161 aaggagacga ttttacatga gctggacaga cgagaacgaa cctataacca acagcgaacc
     5221 aacgagctac gaagagctcg agctagcatt cgcgaaactg cagaagaagt acgaagagaa
     5281 caacgaactc ttacgcctga acaaccggaa attagaccgg ctcgaagaaa ccatgaacaa
     5341 cacgttgaaa gaggaacaga ccaccaacaa gaattatcgc tctaaaacgt taaaagacat
     5401 agaaaacgaa ataaagaaac aacgtgcttt tctcgttcct tatacgcaag aaaggataca
     5461 aactacgatt gatttacaag ttaaaaaact ccatcaagag ctattagacc agcgaaataa
     5521 ggaacagaaa aaggtgctta ttcaacgatc attggatttt cttgccccct tatgttgctt
     5581 agtcctgaca ctgttcgcat tttggttagt atggggaggc taaaatggca ttttatttac
     5641 cctatctact catttttgtt agtatttcag gaagtatttg gctcatctat aaaatttttc
     5701 aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgatttttc tttcttggct
     5761 gtatattttc cttgataatc gtttcaagcg gcttattcgg cgttttagag ggaaataagc
     5821 gcgtttctag aagtatttta ttaggaaatg tcactcaaaa gtatgaatca gcgcgtgata
     5881 aaaagaaaaa agagcaagca ttagcacaaa aaatggaaga gtttaccact tgttataagg
     5941 aaatgaacga tatttttgtg aatcaagaaa aacgtttgac ggataaaaac atggaaaaac
     6001 tcactcgtct ttatcaaaac ttgccagaaa aacagcaaaa agaggttcaa gacaactatg
     6061 agcgaaccaa aaaagacgtg caatatgtaa aagatacaaa gatagaagaa gcctgtagtg
     6121 atctatttgg agacacaaac ccttggtttg ccagtgaaga agaaaaaaaa gaaaaacaac
     6181 aatctgtcac atatgaacgc tacgaaaacc tgtttcaaca agcaactaac atgcaaaatc
     6241 cgacaaaaaa agaaaccgcc ttaaactatc taaaaagtgt caaggaatgg ttagatcagc
     6301 aacagcaaaa ttgaatttga ttgaaactca tgttaaactg aatgtgcaaa tgatcaaaca
     6361 ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttt caggttgatg
     6421 tcgcctttac tgattttact tcttgtttcg gttattcaac caataactga ataaagagac
     6481 acaaactcct acaagaaggg ggcaaatgaa ctgttcaaac attagataca cctccttcca
     6541 gtcgcaagac tgtcggatag gcgacaagtt attataccct acaaaattaa atctgtgtgg
     6601 cgattttaag tataccttat tttcgggttg ttagggcttg ccctgaccgc ctgtaaggcg
     6661 cttctcgact agattaaggg agttaatcaa gaattgtctt agaacggctt agacaagctc
     6721 tcagattctc ccacctcctc tttgaacaac ggttttagca aaggagccgg aggcgactgc
     6781 gcaggtacaa tgtgagagcg tagcgatcac acctttttag cgaccgtagg gagcaagttt
     6841 tctagtttag aaaaattcgc gaaccttttt ggaggtaaaa gtaggagttt tttaggcggg
     6901 aaattaaatg tgctcttaag atatactgtt agatatactg ttagatatac tgttaataat
     6961 aactgttagg gattagcaac cttatgggag agtaagtatt gacataggta gtgggggata
     7021 aattgtcaca ctatggggga taaattgtca cactatgggg gataaattgt cacgctatgg
     7081 gggataaatc gtcacactat gggggataaa ttgtatttta caccctctta tgtctatgat
     7141 aacataattt attgaggtga ttttta
//
