LOCUS       CP063584                9081 bp    DNA     circular BCT 04-APR-2022
DEFINITION  Enterococcus faecium strain BMT-4-1-11 plasmid pRL-1-11_05, complete
            sequence.
ACCESSION   CP063584
VERSION     CP063584.1
DBLINK      BioProject: PRJNA669626
            BioSample: SAMN16483160
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9081)
  AUTHORS   Both,A., Kruse,F., Mirwald,N., Franke,G., Christner,M., Huang,J.,
            Hansen,J.L., Kroger,N., Berneking,L., Lellek,H., Aepfelbacher,M. and
            Rohde,H.
  TITLE     Population dynamics in colonizing vancomycin-resistant Enterococcus
            faecium isolated from immunosuppressed patients
  JOURNAL   J Glob Antimicrob Resist 28, 267-273 (2022)
   PUBMED   35134550
REFERENCE   2  (bases 1 to 9081)
  AUTHORS   Both,A., Kruse,F., Christner,M., Huang,J., Mirwald,N., Franke,G. and
            Rohde,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) Institute of Medical Microbiology, Virology
            and Hygiene, University Medical Center Hamburg-Eppendorf,
            Martinistr. 52, Hamburg, Hamburg 20246, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Trycycler v. v0.3.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 377.3x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/27/2020 16:29:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.13
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,026
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 54 of 134
            Pseudo Genes (incomplete)         :: 70 of 134
            Pseudo Genes (internal stop)      :: 28 of 134
            Pseudo Genes (multiple problems)  :: 17 of 134
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="BMT-4-1-11"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pRL-1-11_05"
                     /geo_loc_name="Germany: Hamburg"
                     /lat_lon="53.33 N 10.1 E"
                     /collection_date="2015-01-07"
                     /collected_by="Institute of Medical Microbiology, Virology
                     and Hygiene, University Medical Center Hamburg-Eppendorf"
     gene            1..933
                     /locus_tag="IQN12_15500"
     CDS             1..933
                     /locus_tag="IQN12_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UOE09052.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIRF
                     YFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIHGE
                     VDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWAEFS
                     KEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFTW
                     KAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEADFKKQQ
                     QTEKEEAEKQSISNELKQDLLENLQNLFD"
                     /name="replication initiation protein"
     gene            950..1420
                     /locus_tag="IQN12_15505"
     CDS             950..1420
                     /locus_tag="IQN12_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="UOE09053.1"
                     /translation="MTDKTIKELAEEFHVSKQAIRKRLSADFRENYVQTVTRNGVKTLV
                     VSYKGYLLLKQHFSGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLDQQ
                     QQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKENNNAKTTEKKWWKFW"
                     /name="DUF536 domain-containing protein"
     gene            1504..1808
                     /locus_tag="IQN12_15510"
                     /pseudo=""
     CDS             1504..1808
                     /locus_tag="IQN12_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /name="helix-turn-helix transcriptional regulator"
     gene            2083..3264
                     /locus_tag="IQN12_15515"
     CDS             2083..3264
                     /locus_tag="IQN12_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="UOE09054.1"
                     /translation="MKNNRIYKNQLNYGLLLPVFLLCIIGLLSLYVALYHDSYTNSKIT
                     KELIKQVLWYSIGFTSAITIYFFNPKILWKLTPIFYGLCIIMLLLLLKFYNPQLAVLTG
                     AKNWFYIAGFSFQPTELVKIGYTLMLAKITVNYQQKSITSNTKDDWIYLLKTFIATLPI
                     GILLFFQNDLGTMLVFIVIYIFILFTSNINIKIIAPIIAIGVVLSLIFLYLVISDTGRD
                     FLLKIGFHDYQFKRIDSWLNPFLDPNGSSYQLANSLIAIGSGGLLGTGFNVSNIHVPVR
                     ESDMIFSVIGENFGFIGSCLVIFLYFYLIYQMYKTCFKSNNLFFTYIGIGIVAMIFFHV
                     FENIGASIGLLPLTGIPLPYISQGGSAILSNFLGLGFMLCCSRYTNEKKGTQK"
                     /name="FtsW/RodA/SpoVE family cell cycle protein"
     gene            3354..3623
                     /locus_tag="IQN12_15520"
     CDS             3354..3623
                     /locus_tag="IQN12_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="UOE09055.1"
                     /translation="MKKTKLLVASLCLFSSLLAFTPSVSFSQNGGVVEAAAQRGYIYKK
                     YPKGAKVPNKVKMLVNIRGKQTMRTCYLMSWTASSRTAKYYYYI"
                     /name="bacteriocin"
     gene            4429..4602
                     /locus_tag="IQN12_15525"
     CDS             4429..4602
                     /locus_tag="IQN12_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOE09056.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNRELKLNSNYYNS
                     PNEELFVKETDK"
                     /name="hypothetical protein"
     gene            5052..5276
                     /locus_tag="IQN12_15530"
     CDS             5052..5276
                     /locus_tag="IQN12_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="UOE09047.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEKC
                     WVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
                     /name="bacteriocin hiracin-JM79"
     gene            5296..5583
                     /locus_tag="IQN12_15535"
     CDS             5296..5583
                     /locus_tag="IQN12_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="UOE09048.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKSV
                     GEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
                     /name="bacteriocin immunity protein"
     gene            6024..6404
                     /locus_tag="IQN12_15540"
     CDS             6024..6404
                     /locus_tag="IQN12_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UOE09049.1"
                     /translation="MSEQNQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            6386..7300
                     /locus_tag="IQN12_15545"
     CDS             6386..7300
                     /locus_tag="IQN12_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="UOE09050.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRT
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGKEYAEKIAPEYQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLDKCYDINDEICLTYGLSITERGKKEEKRTMSEIKLKEK
                     NKPVWKDEIRFAIDQTMKNKKTRTYDQFCDSLKTFGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARTSIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            7540..8229
                     /locus_tag="IQN12_15550"
     CDS             7540..8229
                     /locus_tag="IQN12_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOE09051.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGAKIRFKRFFLLG
                     CIFSLIIVSSGLLGVLEGNKRVSRSILLGNVTQKYESARNKKKKEQALAQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYEQTKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYENLFQQATNIQSPTKKETALNYLESVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     BGC             52..9081
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2..933
                     /name="rep_cluster_1249"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.786"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6387..7297
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.013"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggcaaatg aaatcgtaaa gtatgaacca gaattaaata ccattccttt aagaaaattt
       61 accccaatag aaatgaactt gtttttttct attgtctcta gaatgcgcga taaaggtaat
      121 caaaaaatac gcttttattt cagccaactt aaagatttaa gtgcgtataa gcagactgca
      181 ggagaagtct ttataaaaga tttggcaaga acatacgatc atttgatgga tttacgtttc
      241 ggaagaaaaa gcaatggtgg attaaccata gaacgattta caatgtttac tgattttgaa
      301 attcatggag aagtcgataa cccttatgta gatataaaag tccacgaaaa agctttgcct
      361 ttacttaata atctagaaag ctgggtacgt ttctctttaa cagaatttcg agatttaaat
      421 agcagttatg ctaaaacaat gtttagattg ctaaaacaat accgaactca aggatgggct
      481 gaattttcta aggaagattt tttagagcta ttagatattc ctaaaagcta tagacaaaca
      541 aatataaatc agtttgtact caaaccaata aaagaagaac taactccact atttaaagga
      601 ttaactattc gtaaaaaata tggtaaaggt cgaggtaaac ctgttattgg ttatcgtttt
      661 acttggaaag ctgaaatcaa ccatgctgac gatttttcaa aagggaagca agaagattta
      721 cgtataaaat tatttaatat tgaacacaat ggagaattaa cacaaaaaga aaaatggcgt
      781 gctaaagatc gaatactaaa tcttccgtta ggtactcatg aagctgattt taaaaaacaa
      841 caacaaaccg aaaaagaaga agcggaaaaa caatcaatct caaacgagct taaacaagat
      901 ttattagaaa acttacaaaa cttattcgat tgaagcataa ggagtattta tgacagataa
      961 aacgatcaaa gaactcgcgg aagaatttca tgtttcaaaa caagcaatta ggaaaaggct
     1021 atcagctgat tttagagaaa actatgtgca aactgttact aggaacggag tgaaaacctt
     1081 agttgtcagc tacaaaggat atttgctttt aaaacaacat ttttcaggta gtaacaccac
     1141 tggtaaccaa gtagaaaccg ttgctagtaa cgatgaaaac tcgattattg tttttttaca
     1201 tgaacagctc caagaaaaag atactcaaat aaaacaaatg caaaagttac tcgaccagca
     1261 acaacaactc accttgcagg caaatcaaca gatcgaaaaa ctgcaagaac agttacaatt
     1321 agcttatacg gaagaacctg aagaagagga attaacttct gtcaataaag aaaacaacaa
     1381 tgcaaaaaca acagaaaaaa agtggtggaa attttggtaa caattaccaa ctttccataa
     1441 agaagaaatc aatttctata gactttatct ttctaagaat aaaaaatatg tttaggtgat
     1501 caaatgacta taagtaaagt aatcaaacaa aaaagaatag aaaaacaact aactcaagaa
     1561 gatatagctg aaatgctttt agtatctaaa aaaaccattt ctaattggga aaatggtcga
     1621 acaataccag atacagaaaa tttaattcag tttgctaagt tatttgattt atcactagac
     1681 aatattttgt taggagaaga aaaaattatg gtaaaaaatc atacaaaaac actaatacta
     1741 ttatttcatt ttgtaatttt tttaagttta gttttcacct taattcagtc cggattcttt
     1801 tattttagcc attcaattgc cacttggtta ttaatatttt ttctaagttt actattaata
     1861 attaatactt taattattat ctttaataaa catagaaact tatttcttca cagcatattt
     1921 ccaattgttt tattaatttt tctatacttt atataaaaag atagaactat ttagtaagat
     1981 aaaaaagtag ctaattgtaa ttttcgtcta aaacttaaaa ctagtaaaaa taaatttatt
     2041 atgttatttt tatttataaa ttatgaaaaa ggacttttcg aaatgaaaaa taatagaatc
     2101 tacaaaaatc agttgaatta tgggctttta ttgccagttt ttctgctatg tatcattgga
     2161 ttactttctt tatatgttgc tttatatcac gactcttaca ctaattcaaa aatcacaaaa
     2221 gaattaatta aacaagtatt gtggtatagt attgggttca cttcagcaat aactatttat
     2281 ttttttaatc ctaaaatttt gtggaaatta actcctatat tttacggatt atgcatcata
     2341 atgttattac ttctcttgaa attctataat ccacaattag cagtcttaac tggagcaaaa
     2401 aattggtttt atattgctgg tttttcgttt caacctactg agttagtcaa aataggatac
     2461 acccttatgc tagctaaaat aaccgtcaat tatcagcaaa aatctattac tagtaatacg
     2521 aaagatgatt ggatttattt acttaaaact tttattgcta ctcttccaat aggaatatta
     2581 ttattttttc agaacgatct aggaacaatg ttagtcttca ttgttattta tatttttatt
     2641 ttgtttacgt ctaacataaa tataaaaata attgccccaa taatcgccat tggtgttgtt
     2701 ttatccttaa tatttttata tttggttata tcagatacag gaagagattt cttattaaaa
     2761 ataggatttc acgactatca gtttaaaaga atagactcat ggttgaatcc atttttagat
     2821 ccaaatggat ctagctatca gttagcaaat agtttaattg ctattggctc tggcggactt
     2881 ttaggtactg gttttaatgt atcaaacatt cacgttcctg ttagagaatc cgatatgata
     2941 ttttctgtaa ttggagaaaa ttttggcttt ataggaagtt gtcttgtaat attcctatac
     3001 ttttatttaa tttatcaaat gtataaaaca tgttttaaaa gcaataactt atttttcacc
     3061 tatatcggta taggcattgt tgcaatgatt ttttttcatg tttttgaaaa tataggtgcg
     3121 agtatcggat tactacctct aactggtatt ccactcccct atattagtca aggtggttct
     3181 gccattctta gtaatttttt ggggttggga tttatgttgt gttgtagtag atatactaac
     3241 gaaaaaaaag gaacacaaaa atgaattcaa aataacactt tatgaataga atgtctattt
     3301 caataaaaat tatgatacct taaagttaat cattaaaaaa ggagtgtcat aatttgaaaa
     3361 aaacaaaatt attggtagct agtttgtgtt tgtttagcag tttattagct ttcacccctt
     3421 ctgtttcatt ttctcaaaat ggtggagtgg ttgaagctgc tgctcaaaga ggatatatct
     3481 acaaaaaata tccaaaaggt gctaaagtgc caaataaagt taaaatgtta gtaaatatta
     3541 gaggtaaaca aactatgcgt acttgttacc ttatgagttg gactgctagt agtagaacag
     3601 ccaaatatta ttactacatt tagtaaaaac aaaacattct ttctatacaa taaaatagcg
     3661 tatagagagg atgtttttaa tttgaaaagg agtggttatc tttatgaaaa atagatcatt
     3721 tgctatatta gtattatccg taattttatt agatatattg ttaataacat tagctttaaa
     3781 aactttaaat ttagtaacaa gttttgtcat tataccagta attataggaa tgtctattta
     3841 tttcatcaaa aaaaataaac aatttttttg aatgaaatta cgggcgaaaa taatcactca
     3901 aaattattaa taaaaacgaa tcaactactt tttctgaaga aacatacaac cgtaaacaac
     3961 aattgttgtt tacggttgta aattatcaaa ctatatggtg acttgttgct taaaataggt
     4021 tgaaatttcg tttatttttt ttgagaaatt ttacccacgt actacccatg tacctatcca
     4081 cgtattaccc atgtaccccc cctgacaaat gggcggaggg gcttgcggcg cttggtatta
     4141 aaggcattct ccgcctccca ggtttctgac cattttctct caacgtggta aaaaccccac
     4201 ttatgagaga aaatggtcag aaacctaaac gatcggcaaa gaaaacatac caagcgccac
     4261 aagcaaaaaa agttagagta acttccagaa aagaaaaatt ttttagaaca acttttgaaa
     4321 atttgatttt gatttatatc cgttcaattt aaacatttga taaactgaag gctagtattt
     4381 aaaatgtgta tacaagtggt agcgttaaaa aaagtggagg tataacctat gttaaaaaaa
     4441 actgtgttgg tactgggttc tatttctcta ggaatattag gttttagccc catgctatta
     4501 tccgtaattg gaaaaaacaa ttccaacaga gaacttaaat taaattcaaa ttattacaac
     4561 agtcctaacg aagaattgtt tgtaaaagaa acggataaat aataaaaaag actgctcttc
     4621 cgagcagcca gttaaaaaat aatgagtgtt tattcttgaa aaagcaacgc gcttatgaag
     4681 cgctaccaaa aatttatcat attttatgaa aaataacacc ccagtaaaaa atgactggta
     4741 aaaattcaca aagaaacgga aagaagcaac tgcagaaaat caagccttaa aaagagcttt
     4801 tgacctagac caatcgaaaa tcacaaagtg agcctctgta tcggttgata caagggctac
     4861 ttggcatagt atacattatg tctactagct gtttaaataa ctgatcttat aattttattt
     4921 aaacgattat gaattcaaaa ctacttttta tgacgttaat cattgttata taataaaatg
     4981 ttatataata aaattactaa gattatttaa tataacaagc aattttaaaa aaatgaaaag
     5041 gaaaaacaat catgaaaaag aaagtattaa aacattgtgt tattctagga atattaggaa
     5101 cttgtctagc tggcatcggt acaggaataa aagttgatgc agctacttac tatggaaatg
     5161 gtctttattg taacaaagaa aaatgttggg tagattggaa tcaagctaaa ggagaaattg
     5221 gaaaaattat tgttaatggt tgggttaatc atggtccatg ggcacctaga aggtagtggt
     5281 agggaggtta ttataatgga ttttactaaa gaagaaaaac ttttaaatgc aattagtaaa
     5341 gtatacaatg aagcaactat agatgactat cctgacttaa aagaaaagct ctttctttat
     5401 tctaaagaaa tcagtgaggg aaaaagtgtt ggtgaagtta gtatgaaatt aagtagtttt
     5461 cttggaagat atattttaaa acataaattt ggattaccta aatctttaat agaattacaa
     5521 gaaattgtta gtaaggaatc tcaagtatat agaggatggg cttctattgg tatttggagt
     5581 taattctttg gaataaaatg aaaatgggac tgaagaatca attctgattc atcagtccca
     5641 taaattatag agccgaaaaa ttatcatatt tatagattta ttaatagtaa atggcataag
     5701 aatctttgaa caacgaaaaa aatgtagctc tacctaatta ttgatttgca acaatttcac
     5761 cgaaggtgaa acaagcgaaa ctttacaaaa agtggctagc atttgctagt cttttttgta
     5821 ttgtggagag tggggggagg gggattcaag ggggagcttg cgcccctttg acaagcggtt
     5881 atttgcacca aatacgtagg tatttggcta gcaaatatag agcttgccaa acccttttgg
     5941 tgagctttac tcattgtacc tttttaggta caatgagtat tgcgaacgat acgtccgtag
     6001 gatgtactga aagggtggga cttatgagcg aacaaaatca aaatttggct agcgatcatt
     6061 ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg agcgaatccg
     6121 aattcgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg gctttcgtga
     6181 aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac attgcccagg
     6241 caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgct aagtggtgca
     6301 atacacacca acacgacgtt gctcctggag acgcccaacg cttatccgaa aacctaaaaa
     6361 tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagccgaa ccacttcatg
     6421 tagccgagcg atcaattacg cagaaccacg agcaaccgtc aaaacaggaa taaactgtga
     6481 cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa aagacgatcg
     6541 tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg ctgaaaaagc
     6601 caaccaacta gggaaagaat acgctgaaaa aatcgcacca gaataccaaa tcgcgattta
     6661 cacgcacaca gataaagacc atatccacaa tcacattgtc attaattcgg tcaatttaga
     6721 aacaggaaaa aagtttcaag cacatggtca agcattccta gataagtgtt acgatatcaa
     6781 cgacgaaatt tgtttaactt atggtttaag tatcactgaa cgaggaaaaa aagaagaaaa
     6841 acgaaccatg tctgaaatca aattaaaaga aaaaaataaa ccggtttgga aagacgaaat
     6901 tcggtttgcg attgatcaaa ccatgaaaaa caagaaaacc agaacctatg accaattttg
     6961 tgattccctc aaaacattcg gcattcattg cttcaatcgc gggaaaaatt tcacttacga
     7021 attgatccac aaaaagaaaa aagtacgatc caacagacta ggaaaggact acgaaaagga
     7081 gacgatttta catgagctgg acagacgaga acgaacctat aaccaacagc gaaccaacga
     7141 gctacgaaga gctcgaacta gcattcgcga aactgcagaa gaagtacgaa gagaacaacg
     7201 aactcttacg cctgaacaac cggaaattag accggctcga agaaatcatg aacaacacgt
     7261 tgaaagagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa gacatagaaa
     7321 acgaaataaa gaaacaacgt gcttttctcg ttccttatac gcaagaaagg atacaaacta
     7381 cgattgattt acaagttaaa aaactccatc aagagctatt agaccagcga aataaggaac
     7441 agaaaaaggt gcttattcaa cgatcattgg attttcttgc ccccttatgt tgcttggtcc
     7501 taacactgtt cgcattttgg ttagtatggg gaggctaaaa tggcatttta tttaccctat
     7561 ctactcattt ttgttagtat ttcaggaagt atttggctca tctataaaat tttccaaaca
     7621 agatattcat taaaaggcgc taaaattcga tttaagcgat ttttcttgct tggctgtata
     7681 ttttccttga taatcgtctc aagcggctta ttaggcgttt tagagggcaa taagcgtgtt
     7741 tctagaagta ttttattagg aaatgtcact caaaagtatg aatcagcacg taataaaaag
     7801 aaaaaagagc aagcattagc acaaaaaatg gaagagttta ccacttgtta tgaggaaatg
     7861 aacgatattt ttgtgaatca agaaaaacgt ttgacggata aaaacatgga aaaactcact
     7921 cgtctttatc aaaacttgcc agaaaaacag caaaaagagg ttcaagacaa ctatgagcaa
     7981 accaaaaaag acgtgcaata tgtaaaagat acaaagatag aagaagcctg tagtgatcta
     8041 tttggagaca caaacccttg gtttgccagt gaagaagaaa aaaaagaaaa acaacaatct
     8101 gtcacatatg aacgctacga aaacctgttt caacaagcaa ctaacataca aagtccaacg
     8161 aaaaaagaaa cggcattaaa ttatctagaa agtgtcaagg aatggttaga tcagcaacaa
     8221 caaaattgaa tttgatttaa aatcatgtta aactaaacat gcaaataggt aatcatgaga
     8281 tacacgaaag ccctagagaa gtcggattct ctagggcttt tttttttggc tgatgtcgcc
     8341 ttagttgatt ttacgtcttg tttcggttat tcagccaata actgaataga gagtttagaa
     8401 gctcaacaag aagggcgcaa ataaagttgg taatcataag atacacctcc ttccagtcgc
     8461 aagactgtcg gataggcgac aagttattat atcttaaaaa attaaatttg tgaggtgttt
     8521 ttaagtatta cttgttttag ggttgttagg gcttgccctg accgcctgta gggcgcttta
     8581 aattccagat aaaggttttt atactaagac tgtcttaaaa cggcttagac aagctcttag
     8641 agcctcaacc ctcctttttg aacaatggtt ttagcaaagg agctggaggc gactgcgcag
     8701 gtataatgtg agagcgtagc gatcacacct ttttggcgac cgtagggagc gaggttttga
     8761 attagcagtt tagaaaagtt cgcgaacctg tttaaagaaa aaagaattgt tttttaggtg
     8821 ggaaattttg tctccctctt ttataatctt tttttaaacc tctttttaaa cctctttacc
     8881 ttcggctgtg tccttactcc cccaagggat acaaaatttt ataggcatga aattatctgt
     8941 ttataggcat gaaattatct gtttataggc atgaaattat ctgtttatag gcatgaaatt
     9001 atctgtttta ggtattgttt ttagacataa tatgtcctaa aattatgtca ataaaatcat
     9061 ttagactgga gtaacaaata a
//
