LOCUS       CP063583               10613 bp    DNA     circular BCT 04-APR-2022
DEFINITION  Enterococcus faecium strain BMT-4-1-11 plasmid pRL-1-11_04, complete
            sequence.
ACCESSION   CP063583
VERSION     CP063583.1
DBLINK      BioProject: PRJNA669626
            BioSample: SAMN16483160
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10613)
  AUTHORS   Both,A., Kruse,F., Mirwald,N., Franke,G., Christner,M., Huang,J.,
            Hansen,J.L., Kroger,N., Berneking,L., Lellek,H., Aepfelbacher,M. and
            Rohde,H.
  TITLE     Population dynamics in colonizing vancomycin-resistant Enterococcus
            faecium isolated from immunosuppressed patients
  JOURNAL   J Glob Antimicrob Resist 28, 267-273 (2022)
   PUBMED   35134550
REFERENCE   2  (bases 1 to 10613)
  AUTHORS   Both,A., Kruse,F., Christner,M., Huang,J., Mirwald,N., Franke,G. and
            Rohde,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) Institute of Medical Microbiology, Virology
            and Hygiene, University Medical Center Hamburg-Eppendorf,
            Martinistr. 52, Hamburg, Hamburg 20246, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Trycycler v. v0.3.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 377.3x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/27/2020 16:29:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.13
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 3,116
            CDSs (total)                      :: 3,026
            Genes (coding)                    :: 2,892
            CDSs (with protein)               :: 2,892
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 54 of 134
            Pseudo Genes (incomplete)         :: 70 of 134
            Pseudo Genes (internal stop)      :: 28 of 134
            Pseudo Genes (multiple problems)  :: 17 of 134
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..10613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="BMT-4-1-11"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pRL-1-11_04"
                     /geo_loc_name="Germany: Hamburg"
                     /lat_lon="53.33 N 10.1 E"
                     /collection_date="2015-01-07"
                     /collected_by="Institute of Medical Microbiology, Virology
                     and Hygiene, University Medical Center Hamburg-Eppendorf"
     gene            1..951
                     /locus_tag="IQN12_15440"
     CDS             1..951
                     /locus_tag="IQN12_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="UOE09038.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRL
                     TFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIRGT
                     AKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVVGYQF
                     TFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQI
                     KQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
                     /name="RepB family plasmid replication initiator protein"
     gene            972..1505
                     /locus_tag="IQN12_15445"
     CDS             972..1505
                     /locus_tag="IQN12_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="UOE09039.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLREQL
                     ALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEQLQLTYTEEAPENDSTTLSEATETID
                     KQIDTEKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            1521..1691
                     /locus_tag="IQN12_15450"
     CDS             1521..1691
                     /locus_tag="IQN12_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOE09040.1"
                     /translation="MTRKQLLFGSILLGILGFSPMLLSVINKGNKRGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(2006..2467)
                     /locus_tag="IQN12_15455"
     CDS             complement(2006..2467)
                     /locus_tag="IQN12_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="UOE09041.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPDI
                     ETLKKICEFYSTNINSILNEDNLKNKQNNLLVLTAIIFNIFVTILGLFELIQMNFFSFT
                     HGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTIFFPLVILIILLS"
                     /name="helix-turn-helix transcriptional regulator"
     gene            2600..2974
                     /locus_tag="IQN12_15460"
     CDS             2600..2974
                     /locus_tag="IQN12_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="UOE09042.1"
                     /translation="MKKFLCLSALMGVLLTSGGIVSATEALNLDVPEDHTAIYGGGMEA
                     TEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKAGV
                     RAYATCNATLTGNTGWWNVY"
                     /name="lactococcin 972 family bacteriocin"
     gene            3028..5154
                     /locus_tag="IQN12_15465"
     CDS             3028..5154
                     /locus_tag="IQN12_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOE09043.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQY
                     KDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKYG
                     TPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFDTI
                     KDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTLSIY
                     HLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINIHNEAENNSLKYWERTT
                     NLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENNKEFFI
                     GQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYILVPKKY
                     KEYAKMIEKNYLEDIQFRLEDSPPQAPYKTPNLKNLKIKLIFVNNNQKYFSFNTLYGKA
                     KDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQINPYIKEFNL
                     ESIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVSFTINSKKIA
                     LKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIFLINLITSIII
                     YKKNSFKYFKENIL"
                     /name="hypothetical protein"
     gene            5151..5771
                     /locus_tag="IQN12_15470"
     CDS             5151..5771
                     /locus_tag="IQN12_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="UOE09044.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTLL
                     NNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAKKF
                     NSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEPTGN
                     LDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
                     /name="ABC transporter ATP-binding protein"
     gene            complement(5929..6837)
                     /locus_tag="IQN12_15475"
     CDS             complement(5929..6837)
                     /locus_tag="IQN12_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="UOE09035.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQL
                     KQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIKII
                     RYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHM
                     IVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALKGITIS
                     VPPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAY
                     SVLLSGAQRRFEGTLRYSLGY"
                     /name="IS982-like element ISEfm1 family transposase"
     gene            7521..7901
                     /locus_tag="IQN12_15480"
     CDS             7521..7901
                     /locus_tag="IQN12_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UOE09045.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            7883..8797
                     /locus_tag="IQN12_15485"
     CDS             7883..8797
                     /locus_tag="IQN12_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="UOE09036.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKKKVRS
                     NKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTFTPEQSEI
                     RPTRRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            9042..9731
                     /locus_tag="IQN12_15490"
     CDS             9042..9731
                     /locus_tag="IQN12_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOE09037.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLVG
                     VIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTACY
                     EDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQTEEA
                     CCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLESVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(9852..9938)
                     /locus_tag="IQN12_15495"
     CDS             complement(9852..9938)
                     /locus_tag="IQN12_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017548328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="UOE09046.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     BGC             1..7974
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          3..951
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.953"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          7884..8794
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.408"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttggcaaatg aaatcgtgaa ataccatcat gaattaaata ccattccgtt gcggaaattt
       61 acaccagttg aaatgaatct atttttctct attgtgtcta gaatgcgcga aaaaggcgat
      121 aaaacagtcc gtcttacttt tgaccaacta aaagatttaa gtaattataa agcaactgct
      181 aataatcgtt tcattaatga tttagaaatg acttatgata aattaatgga tttacgtttt
      241 ggtaaaagaa gtgctaatgg gctcgatata gatagattcg ttatgtttag tgagtttaaa
      301 atacgtggaa ctgctaaaga tccatatgta gacatacaag tttacgaaaa agctttacct
      361 ttactaaaca atttagatga gtgggttcgc tattcactcc aacaatttaa cgaattacaa
      421 agtagctatg caaagactat gtttcgatta ctaaaacaat atcgaactaa aggttttgct
      481 tatttttcta aagaggattt tcatgaacta ttagatattc caaaaagtta ctggaacaac
      541 caagcaaacg ttgataatcg tgttataaag cctatacgac aggaattaac cgcaatcttc
      601 aaaggattat caatcaaaaa gaaatacgga aaagggcgag gaaaacctgt cgttggctat
      661 caatttacgt ttaaaccaga aatcaaaaaa gcagatgatt ttaataaaca tgcaccaacc
      721 aaagaagaac aaaataaagc attggcttta gctgataaac aaaaagaata cgaagaacct
      781 gctacaacaa ttaacgaaaa aatcaaacag aagaaacgag aacttgaact cctccaacaa
      841 atcaaacaac tagaaaaaga gaaagaagct cttgaaaaac aaacagagtc cgcactttcg
      901 aaaaaagaga aaaaaagttt attatctgat ttagataatt tattccagta gcaacctagg
      961 aggcaaccag tatgcaacca caagaaacct ttacaataaa ggaattgtca gaacttttta
     1021 agatgagtag gcaagcaata tcaaaacata tccaaaaatt agattcttcc atgatagcta
     1081 aaaatgagcg tggttataag gttgtattgc gttctggagt attgcaactt gctcgcaact
     1141 taggcaacca aatgttgcta gataggttgc acgagtacga acaagacaac gataaaaatg
     1201 ttaatatacc aagcaaaaac gtggttacaa acaagttgct agatcagttg cgtgaagaaa
     1261 atgaattttt acgagaacaa cttgccctca gagagcaaaa taattctcat atacaaaaac
     1321 tactcgacca gcaacaacaa ctcaccctac aagccaacaa acaaatcgaa aaactgcaag
     1381 aacaactaca gttgacttat acagaagaag ctccagagaa tgactcaacg actctttctg
     1441 aagcaacaga aacaatagac aaacaaattg atactgaaaa aaaatggtgg caactatgga
     1501 aataactgga gggaacattc atgacaagaa aacaacttct attcggttcg attttactag
     1561 gaattctcgg tttcagtccg atgttattgt cagtgatcaa taaagggaat aaacgtggtg
     1621 aattaaaatt gaatccaaat tattacaaca gtcctaacga agaattgttt gtaaaagaaa
     1681 cggataaata ataaaaaaga ctgctcttcc gagcagccag ttaaaaaaat aatgagagtt
     1741 tattcttgaa aaaagcaacg cgcttataaa gcgctaccaa aaatttatca tattttatga
     1801 aaaataacac cccagtaaaa aatgactggt aaaaattcac aaagaaacgg aaagaagcaa
     1861 ctgcagaaaa tcaagcctta aaaagagctt tttacctaga ccaatccaaa atcacaaagt
     1921 gagcccctgt atcggttgat acaagggcta cttggcatac tacacattat gtcaactatg
     1981 caattaaata actaactact tcatattagc ttaataaaat aatcaaaatc actaggggaa
     2041 aaaatattgt ataaaaaaat ttttcatttt ttaaataaga atagtaaata taaaaataat
     2101 ttaagatatc tattactatt aatccagata gagcaaacca agtaaatatt ccgtgtgtaa
     2161 agctaaaaaa attcatttga atcaattcaa ataaacctaa tatagtaaca aatatattaa
     2221 aaataatagc tgtcaatacc agtaaattat tttgtttatt ttttaaatta tcttcattta
     2281 aaatagaatt tatgttagta ctataaaatt cacaaatttt ttttagtgtc tctatatctg
     2341 gtgttgtttt gttattttcc cactttgaaa tagtttgacg tgtaacatta aattttcttg
     2401 ccacatattc ttgagtatac ccttttttca ctcttaattc tcttagctta gatcctaaat
     2461 cctccatttt ttctcctatg taaacgatta tttacataaa attttatcat aaaaatacta
     2521 acgcgccaaa aaagttcttt attttataaa acacactaaa ataaaataat tctagtaaaa
     2581 aagataggag aaataagtta tgaaaaaatt tttatgtctt tctgctttaa tgggtgtctt
     2641 attaactagt ggaggaatag ttagtgctac agaagcttta aatctagatg tacccgaaga
     2701 ccataccgcc atttacggag gaggaatgga agctacagaa gaaggatttt cttctagtaa
     2761 attgagatat gctgctggcg gcggagattt caactgtggg gttaatggat ttaaagttta
     2821 tgcaaattac taccatgcta gagctaaaca tagtgcaaca gctaaaaatg gacgaggcgg
     2881 tcaagtacga tcagttcaga aagctggagt aagagcttat gcaacatgca atgccacact
     2941 tactggaaat actggttggt ggaatgttta ttaaaaaaga gagtttaaac tctctttttt
     3001 atttacattt gaaaggaaat tcaaaatatg ttaaaaaaaa taaacgctgt tttatcactt
     3061 tttgtctgca ttacaatact attcttttct actaatgtta taaatggtat aaagacttct
     3121 gaagcgctat ataaaaatac cgctgaaatt catatgcaat ataaagatga tatggacttt
     3181 aacagttata taaacgcttt gtatgaaata tctacacaag aaaatattaa tatttctcaa
     3241 tatagtttta ctgctgttaa ccaactaact attcttgcta caaaccctaa cgctaacaaa
     3301 cactggaaat tttctaaaaa aaatatatta gcaagaaacg attctaaagt ccattatact
     3361 aataacaaaa attctaagta ccaattaaaa cttccaaata attttttaaa tataaatatt
     3421 tatccttttt ctcatgtgga aaatattgga ttaagtgaaa tattatatgt acaaggaaac
     3481 tcaaacaaat taattcctgt actgaaaaaa tatggaacac ctactatttc caatatcaat
     3541 caatcagata cttttcaaat taatactaat attgttcttg ttatctgcta tcttagtata
     3601 tttattgtaa ttactacaat tatatttgct ttttcaaaaa tgaaagaaat cactctaaaa
     3661 aaaatgttag gctataactc ttttgatact ataaaagatt ctattaaaga gttaaacctc
     3721 ttattttgtt ctttgattat aactttcatt ttatctattt ttttgagcat atattacagt
     3781 aacgaaaatt ttttcctatc atttcttcta tgtagtattt ttcattttat aattagtttt
     3841 ttatttatca tagttactct aagtatctat catttatcta gtataaaaaa ttatctaaaa
     3901 aattcacgac cacttaagtt aaatctttgt atattaaata tctgtctatt cttaagcatg
     3961 atccttctgt taatagcttc tacaaaagta ataaatatcc ataatgaagc cgaaaataat
     4021 tctcttaaat attgggaaag aactactaat ttatataaaa caaatattac taatcaatta
     4081 aatagaaaca acaccgtaga agaaaataac tacttgaaaa aagctagtaa atttgtttat
     4141 aaaatacaga agaattataa aacttttatt atcgcgccat acaattacgc aactatccaa
     4201 gaaaataata aagaattttt tattggacaa aaagtttatc caaattttga agactatgtt
     4261 agtcaacctg ctggtgcagg aatatgtgtt gatttaaact atctaaaata ttacaatcct
     4321 atttcttttg aagaatctac tgacttaaat ttaataaact ctgatccact aactacttac
     4381 attctagtac ctaaaaaata taaagaatat gcaaaaatga ttgaaaaaaa ttatttagaa
     4441 gacatacagt ttagacttga agactctcct cctcaagctc cttataaaac tcctaatttg
     4501 aaaaatttaa aaataaaact aatatttgtt aataataacc aaaagtattt tagttttaat
     4561 accttatatg gaaaggcaaa agataactat acaataactg atcccatagt tagagttatt
     4621 aatccagaaa taacagaatc attattttgg ggaaatatat tgactagtga tggtggatta
     4681 ttttttgatc agaaacatac ttcaaaccaa aactttttta atcaaattaa tccgtatata
     4741 aaagaattta acctagaaag tatcataaat ttttcttatt ctgtttttca agaaactggt
     4801 gaattattat ctcaaaaaaa agtaaataaa ttaaatgcat atctacaata tcttttaata
     4861 ttaggattat ttataataat taattttcaa atgatttttg ttagttttac tataaattca
     4921 aaaaaaatag ctttaaaaaa aatatttgga tattcaccaa taagtcaagt tttagattta
     4981 tgtttactcc cttttacaat agagttattt gctactatgt gttttcaatt gatattttca
     5041 aaaaacactt acttgtattt tataattttt atgatctttt taataaatct aattacttct
     5101 attattattt ataaaaaaaa tagttttaaa tattttaagg agaatatttt atgatattat
     5161 cagttgagaa tttcaccctg tcaattaata aaaaaaagat caaacaaaat tggaactttt
     5221 caatatcaga aaaagatttt attatagtaa caggaaaaag tggtagtgga aaaactactc
     5281 tgttaaataa tttaagccta atagataaac aatataccgg taacttattc tattttgaaa
     5341 agaaagtttc taaacatgat atacaacgtc tacgaagaaa tcatattagc tatttatttc
     5401 aaaactatgc tcttcttgaa aatcaaacag tacaatacaa tttcgaatta gcaaaaaagt
     5461 ttaacagtaa tatttccaat gatcatattt ataatttatt aacagatttt tctttgccaa
     5521 aagatatctt aaaagaaaaa atctttctct taagcggtgg agaacaacaa agaatagcat
     5581 taattagaaa tatgttaaaa ccatttgata tattatttgc agatgaacct acaggaaatt
     5641 tagataaaga aaactcaact tttataatga actttctgaa aaatttaaca gaaaaagaaa
     5701 aaaaagccgt tgtactagta acacatgact taaatgttct aaactatgga actaatatta
     5761 tagaattata atttttaccc gtgatgctag ctgatttaaa actagcacca cgggtattta
     5821 ttttatattc catctctcct atcccaaaat ttaaataatc acttaataaa aaggaggcac
     5881 ccaagtgatt ttattgaaaa aagagtatac ccgtggtgct agttaatttt aatatcctaa
     5941 agaatatctc aaagttcctt caaaacgtcg ttgtgctcca cttaatagaa cactgtaagc
     6001 cagtaatatg ctttcaaatc tactctctag ccctttaaca gaacgtgagt tgaaattttg
     6061 acaacctaat ttttctaagg aagaaaaaac agtctcaact gtttttcttt gttttccaag
     6121 taagctgtgg tctagttttt ctgatttatc catgttttta cgtggcggaa cggatatagt
     6181 aatgccctta agggcaagct tttcatggat tttatggcta atatatcctt tgtcccctag
     6241 gatattggga agattcgctt cttccgataa agtctctaac actttcacgt catggacacc
     6301 tggattcgtg attgagtaag taatcggaaa gccagtctta gtaacgatca tatgaatttt
     6361 aagaccataa aaataagatt tttttgttga gttatagcca actttggcta tttgattcaa
     6421 tagttttgct tgtctatttc taactggttt acataatgga ctaggaaaac tatctataat
     6481 tccgactagt tcacctttgg tgagcttctt gatgaaaaag tatcgaataa tcttgatagt
     6541 gtaagctaag tttgaactta agcgagtgaa tcgactccta ctaggaaagt caccattagg
     6601 aaataagacg gaacaaaccg ctctatacgt ggctctttgg ctagtatagc cattaattat
     6661 gccccatata acacaagcaa taatcactgt atcgcgttgc ttcagttgat cagtatttct
     6721 tcggttttta atcttatctg gtacacaatt gtggtatatg ttagaaacag ttctcataat
     6781 ttccttgaat gttataaaag tatctgtata atgttcagta tattttgatg aacgcatatg
     6841 tagtcctcat ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa
     6901 catgtttaga cattttcctc taagaaaaaa ttaactagca ccacgggtaa gagtatgaaa
     6961 aattttatga tagacttatt tattctttgc aattgcccaa aaaattaaac cgtcaattac
     7021 aaaagactta tgaaactaaa tcaactgaag aatgggagag ttattgctag atttttggta
     7081 gaagtaacaa aaagtggaca tattatcata atgactcgcc gaaagtctga taaaaaattg
     7141 gctaaaaata ttgctgaatg tttaataaaa ctatcagttt tagctaaata gttagtagtt
     7201 tacacaaaaa agcatttatg ttttttgttg caaagcaaca atttcatctt cggtgaaata
     7261 agcgaaactt tacaaaaagt ggctagcatt tgctagcttt ttttgtattg tggagcgtgg
     7321 gttttgaggg ggattcaagg gggagcttgc gcccctttga caagcggtta tttgcaccaa
     7381 atacggaggt atttggctag caaatataga gcttgccaag ccctttagtg agctttagtg
     7441 agctttactt attgtacctt tttaggtaca atggtattgc gaacgatacg tccgtaggac
     7501 gtactgaaag ggtgggactt atgagcgaac aaaatcaata tttggctagc aatcattcta
     7561 aaaaatatac ctatcgatcc gagccaaaac aaatcagttt tcgagtgagc gaatccgaat
     7621 ttgcaaagct gaagcagtca gctgaagctt tgcaaatgag tgtgccggct ttcgtgaaag
     7681 ccaaggcaca aaacgcgcgt ctggtgacac cgaaagtggc gccagacatt gctcaggcaa
     7741 tcgcgcgcga tttagccaaa gctggcggga atatcaatca aattgccaag tggtgcaaca
     7801 cacaccaaca cgacgttgat cctggcgacg cccaacgctt atccgaaaac ctaaaaatca
     7861 tgcaaaagga gctacaaaag atatggcaac agttaaagta agccgaacca cttcttgtag
     7921 ccgagcgatc aattacgccg aaccacgagc aaccgtcaaa acagggataa actgtgacat
     7981 tgattatgcc aaaagcgaaa tgaaacaaat tcgcatgctg tatggaaaag acgatcgtgt
     8041 acaagcacac ttattgatcc agtcttttcg accaggagaa attaccgctg aaaaagccaa
     8101 tcaactaggg gcagagtatg cgaaaaaaat agcccctgac caccaaatcg cgatttacac
     8161 acatacagac aaagaccata ttcataacca cattgtcatt aactccgtca atctggaaac
     8221 tgggaacaaa tttcaagccc atggtcaagc atttttaaac aaatgttatg acctaaatga
     8281 cgagatttgt cttgaacatg acttaacaat cactgaacga gggaagaaac ccgaaaaacg
     8341 aaccatgtct gaaatcaaat taaaagaaaa aaatgaacct gtttggaaag acgaaattcg
     8401 gtttgcgatt gatcaaacaa tgaaaaacaa aaaaactcga acgtataacc aattttgtga
     8461 ttctcttaaa acattcggca ttcattgctt gaatcgtggg aaaaatttta cttacgaatt
     8521 gatccacaaa aagaaaaaag tacgatccaa caaactagga aaggattacg aaaaggagac
     8581 gattttacat gagctggaca gacgagaacg agcctacaat caacaacgaa ccaacgagct
     8641 acgaagagct cgagctagca ttcgcgaaac tgcaaaagaa gtacgaagag aacaacgaac
     8701 ttttacgcct gaacaatcgg aaattagacc gactcgaaga aaccatgaac aacacgttga
     8761 acgaggaaca gaccaccaac aagaattatc gctctaaaac gttaaaagac atcgaaaacg
     8821 aaataaagag acaacgtgcc tttcttgcgc cttataccca agaaaggata caaactacga
     8881 tggattcaca agtcaaaaaa ctccaccaag cgctattaga ccagcgaaac caagaacaaa
     8941 aaaaggcttt aattcaacga tcgatggata ttcttgcccc cttatgttgc ttagtactaa
     9001 cattattagc cttttggttg gtatggggag aataaacaga catggcattc tatttaccct
     9061 atctactcat ttctgccagt attttaggaa ttatttggct tagctataag gcttatcaaa
     9121 caagatattc attaaaaggc tctaaaattc gatttaagcg atttttattg gtcggggtta
     9181 tatttgcctt atcaatcgtt ttaagtggtt tattcggcgt tttagagggt aataaacgtg
     9241 tttcaagaag cattttatta ggggatatca ctcaaaagta tgaagcagca tgtgataaaa
     9301 agaaaaaaga gcaagcatta gcacaaaaaa tggaggaatt taccgcttgt tatgaggaca
     9361 tgaacaatat ttttgtggat caagagaaac gtttaacaga taaaaacatg aaaaaactca
     9421 ctcgttttta tcaaaactta ccagaaaaac agcaaaaaga ggtacaagaa aaatatgagc
     9481 aagtaaaaaa agacgtgcaa tattttaaag atacgcagac agaagaagct tgttgtaact
     9541 tatttagcga tacaatccct ttttcaacca gtgaacaaga acgaaaagaa cggcaacaat
     9601 cggtcacata cgaacgttat gaaacactgc ttcagcaagc aacaaacata caaaatccaa
     9661 cgaaaaaaga aacggcatta aattatctag aaagtgtcaa ggaatggttg gatcagcaac
     9721 aacaaaattg aatttgatta gcaatcatgt taaactgaac ctgcaaatga tcaaacatta
     9781 gatacacgaa agccccagag aggtcggatt ctctggggct ttttttcagg ttgatgtcgc
     9841 ctttattgat tttacttctt gtttcggtta ttcaaccaat aactgaataa agagacacaa
     9901 acttctacaa gaagggggca aatgaaccgt tcaaacatta gatacacctc cttccagtcg
     9961 caagactgtc ggataggcga caagttatta taccttacaa aataaaatct gtgtggtgtt
    10021 tttaagtatc ccttattttc gggttgttag ggcttgccct gaccgcctgt aaggcgcttt
    10081 aagaacaaga taaggttttt tacccaagac tgtcttaaaa cgacttagac gagctatcag
    10141 aggcttcccc cacctccgtt actgacttgt ttttagcaaa ggagccttta ggcgactgcg
    10201 cagtacaatg tgagagcata gcgatcacac ttttttagcg accgtaggga gcaaggtttt
    10261 gaattggcag ttttgaaaaa tgcgcgaacc tgttggtaga aaaaagaagt ggtttttagg
    10321 cgggaaattt tgtcaccctc ttttataatc tctttttaaa ccttttttta aacctcttta
    10381 ccgtcggctg tgtccttact ccttcaatgg atacagcgtt ttataaacta cattttgtct
    10441 gcttttaaac tacattttgt ctgcttttaa actacatttt gtctgctttt aaactacatt
    10501 ttgtctgctt ttaaactctt tttttgcact cacgtatgtt ataatatgta ttatcacaga
    10561 caaaacgttg tttataacgt ctgtatccat tgaaggagta aggaggaata tct
//
