LOCUS       CP063567                6173 bp    DNA     circular BCT 04-APR-2022
DEFINITION  Enterococcus faecium strain ICU-3-1 plasmid pZA3-1_05, complete
            sequence.
ACCESSION   CP063567
VERSION     CP063567.1
DBLINK      BioProject: PRJNA669626
            BioSample: SAMN16483163
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Both,A., Kruse,F., Mirwald,N., Franke,G., Christner,M., Huang,J.,
            Hansen,J.L., Kroger,N., Berneking,L., Lellek,H., Aepfelbacher,M. and
            Rohde,H.
  TITLE     Population dynamics in colonizing vancomycin-resistant Enterococcus
            faecium isolated from immunosuppressed patients
  JOURNAL   J Glob Antimicrob Resist 28, 267-273 (2022)
   PUBMED   35134550
REFERENCE   2  (bases 1 to 6173)
  AUTHORS   Both,A., Kruse,F., Christner,M., Huang,J., Mirwald,N., Franke,G. and
            Rohde,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) Institute of Medical Microbiology, Virology
            and Hygiene, University Medical Center Hamburg-Eppendorf,
            Martinistr. 52, Hamburg, Hamburg 20246, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Trycycler v. v0.3.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 648.2x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/27/2020 16:23:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.13
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 3,201
            CDSs (total)                      :: 3,108
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 49 of 124
            Pseudo Genes (incomplete)         :: 64 of 124
            Pseudo Genes (internal stop)      :: 20 of 124
            Pseudo Genes (multiple problems)  :: 8 of 124
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="ICU-3-1"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pZA3-1_05"
                     /geo_loc_name="Germany: Hamburg"
                     /lat_lon="53.33 N 10.1 E"
                     /collection_date="2019-03-01"
                     /collected_by="Institute of Medical Microbiology, Virology
                     and Hygiene, University Medical Center Hamburg-Eppendorf"
     gene            join(5829..6173,1..36)
                     /locus_tag="IQN15_15960"
     CDS             join(5829..6173,1..36)
                     /locus_tag="IQN15_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UOE03141.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            18..932
                     /locus_tag="IQN15_15965"
     CDS             18..932
                     /locus_tag="IQN15_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="UOE03142.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKKKVRS
                     NKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTTEQPEI
                     RPTRRNHDQHVERGADYQQELSF"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            880..1170
                     /locus_tag="IQN15_15970"
     CDS             880..1170
                     /locus_tag="IQN15_15970"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOE03143.1"
                     /translation="MTNTLKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTT
                     IDSQVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
                     /name="hypothetical protein"
     gene            1177..1866
                     /locus_tag="IQN15_15975"
     CDS             1177..1866
                     /locus_tag="IQN15_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOE03144.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLPG
                     VIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQTEEA
                     CYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(1987..2073)
                     /locus_tag="IQN15_15980"
     CDS             complement(1987..2073)
                     /locus_tag="IQN15_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017548328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="UOE03146.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            2710..3654
                     /locus_tag="IQN15_15985"
     CDS             2710..3654
                     /locus_tag="IQN15_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="UOE03145.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKINGD
                     VENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFAYFS
                     KEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQIKQ
                     LEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
                     /name="RepB family plasmid replication initiator protein"
     gene            3675..4208
                     /locus_tag="IQN15_15990"
     CDS             3675..4208
                     /locus_tag="IQN15_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="UOE03138.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLREQL
                     ALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATETMD
                     EQTKNWKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            4178..4393
                     /locus_tag="IQN15_15995"
     CDS             4178..4393
                     /locus_tag="IQN15_15995"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOE03147.1"
                     /translation="MEKMVAAMEMKKEILMTKKHLLFGSILLGILSFSPMLLSVANKGN
                     KKGELKLNPNYYNSQNEEVFVEKLDK"
                     /name="hypothetical protein"
     gene            4848..5072
                     /locus_tag="IQN15_16000"
     CDS             4848..5072
                     /locus_tag="IQN15_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="UOE03139.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEKC
                     WVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
                     /name="bacteriocin hiracin-JM79"
     gene            5092..5379
                     /locus_tag="IQN15_16005"
     CDS             5092..5379
                     /locus_tag="IQN15_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="UOE03140.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKSV
                     GEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
                     /name="bacteriocin immunity protein"
     BGC             1..6173
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2711..3654
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          19..929
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.421"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaggagctac aaaagatatg gcaacagtta aagtaagccg aaccacttct tgtagccgag
       61 cgatcaatta cgccgaacca cgagcaaccg tcaaaacagg gataaactgt gacattgatt
      121 atgccaaaag cgaaatgaaa caaattcgca tgctgtatgg aaaagacgat cgtgtacaag
      181 cacacttatt gatccagtct tttcgaccag gagaaattac cgctgaaaaa gccaatcaac
      241 taggggcaga gtatgcgaaa aaaatagccc ctgaccacca aatcgcgatt tacacacata
      301 cagacaaaga ccatattcat aaccacattg tcattaactc cgtcaatctg gaaactggga
      361 acaaatttca agcccatggt caagcatttt taaacaaatg ttatgaccta aatgacgaga
      421 tttgtcttga acatgactta acaatcactg aacgagggaa gaaacccgaa aaacgaacca
      481 tgtctgaaat caaattaaaa gaaaaaaatg aacctgtttg gaaagacgaa attcggtttg
      541 cgattgatca aacaatgaaa aacaaaaaaa ctcgaacgta taaccaattt tgtgattctc
      601 ttaaaacatt cggcattcat tgcttgaatc gtgggaaaaa ttttacttac gaattgatcc
      661 acaaaaagaa aaaagtacga tccaacaaac taggaaagga ttacgaaaag gagacgattt
      721 tacatgagct ggacagacga gaacgagcct acaatcaaca acgaaccaac gagctacgaa
      781 gagctcgagc tagcattcgc gaaactgcaa aagaagtacg aagagaacaa cgaactctta
      841 cgactgaaca accggaaatt agaccgactc gaagaaacca tgaccaacac gttgaaagag
      901 gagcagacta ccaacaagaa ttatcgttct aaaacgttac aagacataga aaatgaaata
      961 aagaaacaac gtgcttttct tgcaccttat accaaagaaa gaatacaaac tacaattgat
     1021 tcacaagtca aaaaattcca ccatgcgcta ttaaaccagc gaaataaaga acagaaaaaa
     1081 gtaattatgc agcgatcaat ggatattctt gcccccttat gttgcttaat catgacactg
     1141 ttagcctttt ggctggtatg gggagaataa atagaaatgg cattctattt accctatcta
     1201 ctcatttttg ccagtatttt aggaattatt tggcttagct ataaggctta tcaaacaaga
     1261 tattcattaa aaggctctaa aattcgattt aagaggtttt tattgccagg ggttatattt
     1321 acattatcaa tcgttttaag tggtttattc ggcgttttag agggaaataa acgtatttca
     1381 agaagtattt tattagggga tatcactcaa aagtatgaag cagcatgtga taaaaagaaa
     1441 aaggagcaag tgttaacaca aaaaatggaa gagtttacca cttgttatga ggaaatgaac
     1501 gatatttttg tgaatcaaga aaaacgtttg acggataaaa acatggaaaa actcactcgt
     1561 ctttatcaaa acttgccaga aaaacagcaa aaagaggttc aagacaacta tgagcgaacc
     1621 aaaaaagacg tgcaatattt taaagatacg cagacagaag aagcttgtta taatttattc
     1681 agtgatacaa tccctttttc aactagtgaa caggaacgaa aagaacggca acaatcagtt
     1741 acctacgaac gttataaagc actgcttcag caagcaacga acatacaaaa tccaacgaaa
     1801 aaagaaacgg cattaaatta tctaaaaagt gtcaaggaat ggttagatca gcaacagcaa
     1861 aattgaattt gatcggcaat catgttaaac taaacatgca aatgatcaaa cattagatac
     1921 acgaaagccc cagagaggtc ggattctctg gggctttttt tcaggttgat gtcgccttta
     1981 ttgattttac ttcttgtttc ggttattcaa ccaataactg aataaagaga cacaaacttc
     2041 tataagaagg gggcaaatga acaattcaaa cattagatac acctccttcc agtcgcaaga
     2101 ctgtcggata ggcgacaagt tattatatct taaaaaatta aacttgtgtg gtgtttttaa
     2161 gtatcgctta ttttagggtt gttagggctt gccctgaccg cctgtaaggc gctttaaagc
     2221 tagataagga tttttatcca agactgtctt aaaacggctt agataagcat tcagaaccgc
     2281 tcaaaggcct ctttaccaac atgtttttag caaaggagcc tttaggcgac tgcgcagtac
     2341 aatgtgagag cgtagcgatc acaccttttt ggcgaccgta ggaagcaagg ttttggattg
     2401 gcagttttga aaaatgcgcg aacctgttta aagaaaaaag aagtggtttt taggcgggaa
     2461 aattagcccc cctcttttta tatctctttt taaacctctt tttaaacctc tttaccgtaa
     2521 gctgtgtcct tactctacca atggatacag aatgttatga aggacatttt gtctgtttat
     2581 gaaggacatt ttgtctgttt atgaaggaca ttttgtctgt ttatgaagga cattttgtct
     2641 gtttgaacgt caaatatgaa tgtcttgaca tttaaaaaat gtcattcata attggattga
     2701 ggtgattata tggctaatga gattgtaaaa taccaccatg aattaaatac aattccttta
     2761 agaaaattta caccggttga aatgaatttg tttttttcca tcgtgtctag aatgcgtgaa
     2821 aaaggggata agacagttcg ttttacgttt gaccaactaa aagatttaag taactacaaa
     2881 gcaactgcaa atgtacgatt tgtagaagat ttaaaagcaa cttatgaaaa aatcttatct
     2941 ttacgctttg gtcgtagaag tgagtctggg ctttcttacg atatgtttgt aatgtttaca
     3001 gaatttaaaa tcaacgggga cgtagaaaat ccttacgtag atatacgaat ttacgaaaaa
     3061 gcactgcctt tgttaaataa tttagacgag tgggtacgct attctttaca acaattcaac
     3121 gagctacaaa gtggctatgc aaagactatg tttcgtctgt taaagcaata cagaaccaaa
     3181 ggtttcgcat atttctccaa agaagacttt cttgaattat tagatattcc taaaagttat
     3241 aagcaacctg atatagataa acgagttata aaacctatac gtcaagaact aactgcaatc
     3301 ttcaaaggac taacaatcaa aaagaaatac ggaaaaggac ggggaaaacc tgttattggc
     3361 tatcaattta cgttcaaacc tgagatcaaa aaagcagacg actttaacaa acatgcacca
     3421 accaaagaag accaaaataa agcattggct ttagctgata aacaaaaaga atacgaagaa
     3481 cccgctacaa caattaacga aaaaatcaaa cagaagaaac gagaacttga actcctccaa
     3541 caaatcaaac aactagaaaa agagaaaaaa gctcttgaaa aacaaacaga gtccgcactt
     3601 tcggaaaaag agaaaaaaag tttattatct gatttagata atttattcca gtagcaacct
     3661 aggaggcaac cagtatgcaa ccacaagaaa cctttacaat aaaggaattg tcagatcttt
     3721 ttaagatgag taggcaagca atatcgaaac atatccaaaa attagattct tccatgatag
     3781 ctaaaaatga gcgtggttat aaggttgtat tgcgttctgg agtattgcaa cttgctcgca
     3841 acttagacaa tcaaaggttg ctagataagt tgcataaaca tgaacaaaac aacgataaaa
     3901 atgcttatat accaaacaaa aacgtggttg caaacaagtt gctagatcag ttgcgtgaag
     3961 aaaatgaatt tttacgagaa caacttgccc tcaaagagca aaataattct catatacaaa
     4021 aactactcga ccagcaacaa caactcaccc tgcaagccaa ccaacaaatc gaaaaacttc
     4081 aggagcaact gcagttgact tatacggaag agtcttcaga gaatagttca acgactcttt
     4141 ctgaagcaac agaaacaatg gacgaacaaa caaagaattg gaaaaaatgg tggcagctat
     4201 ggaaatgaag aaggaaatac ttatgacaaa aaaacacttg ctattcggtt ctatcctact
     4261 aggaatatta agctttagcc caatgttatt atcagtggcc aacaaaggaa ataaaaaagg
     4321 ggaactaaaa ctcaatccta attactacaa cagtcaaaat gaggaagtat ttgtagaaaa
     4381 acttgataaa taagaaaaga ctgctcgtac gagcagtctt tctgaaagag attatcctat
     4441 tattaacctt agaaaagaat aataaatgaa tatgagatat gtaaaaagca taacaaacac
     4501 aaacaaaaat aacaagtatt taaaaaatga ccgatcaata ttcacaaaga attagaaaga
     4561 aacaaccgca gaaaaccaag ccttaaaaag agcttttaac ctagaccaat aaaaattcac
     4621 aaaatcagcg cctggatccc ttggtacagg cgttacttgg tagtatacac attatgtcta
     4681 ctagctgttt aaataactga tcttataatt ttatttaaac gattatgaat tcaaaactac
     4741 tttttatgac gttaatcatt gttatataat aaaatgttat ataataaaat tactaagatt
     4801 atttaatata acaagcaatt ttaaaaaaac gaaaaggaaa aacaatcatg aaaaagaaag
     4861 tattaaaaca ttgtgttatt ctaggaatat taggaacttg tctagctggc atcggtacag
     4921 gaataaaagt tgatgcagct acttactatg gaaatggtct ttattgtaac aaagaaaaat
     4981 gttgggtaga ttggaatcaa gctaaaggag aaattggaaa aattattgtt aatggttggg
     5041 ttaatcatgg tccatgggca cctagaaggt agtggtaggg aggttattat aatggatttt
     5101 actaaagaag aaaaactttt aaatgcaatt agtaaagtat acaatgaagc aactatagat
     5161 gactatcctg acttaaaaga aaagctcttt ctttattcta aagaaatcag tgagggaaaa
     5221 agtgttggtg aagttagtat gaaattaagt agttttcttg gaagatatat tttaaaacat
     5281 aaatttggat tacctaaatc tttaatagaa ttacaagaaa ttgttagtaa ggaatctcaa
     5341 gtatatagag gatgggcttc tattggtatt tggagttaat tctttggaat aaaatgaaaa
     5401 tgggactgaa gaatcaattc tgattcatca gtcccataaa ttatagagcc gaaaaattat
     5461 catatttata gatttattaa tagtaaatgg cataagaatc tttgaacaac gaaaaaaatg
     5521 tagctctacc taattattga tttgcaacaa tttcaccgaa ggtgaaacaa gcgaaacttt
     5581 acaaaaagtg gctagcattt gctagtcttt tttgtattgt ggagagtggg ggggaggggg
     5641 attcaagggg gagcttgcgc ccctttgaca agcggttatt tgcaccaaat acgtaggtat
     5701 ttggctagca aatatagagc ttgccaagcc ctttagtgag ctttagtgag ctttacttat
     5761 tgtacctttt taggtacaat ggtattgcga acgatacgtc cgtaggacgt actgaaaggg
     5821 tgggacttat gagcgaacaa aatcaatatt tggctagcaa tcattctaaa aaatatacct
     5881 atcgatccga gccaaaacaa atcagttttc gagtgagcga atccgaattt gcaaagctga
     5941 agcagtcagc tgaagctttg caaatgagtg tgccggcttt cgtgaaagcc aaggcacaaa
     6001 acgcgcgtct ggtgacaccg aaagtggcgc cagacattgc tcaggcaatc gcgcgcgatt
     6061 tagccaaagc tggcgggaat atcaatcaaa ttgccaagtg gtgcaacaca caccaacacg
     6121 acgttgatcc tggcgacgcc caacgcttat ccgaaaacct aaaaatcatg caa
//
