LOCUS       CP063561                4305 bp    DNA     circular BCT 04-APR-2022
DEFINITION  Enterococcus faecium strain BMT-1-1-10 plasmid pIN-1-10_06, complete
            sequence.
ACCESSION   CP063561
VERSION     CP063561.1
DBLINK      BioProject: PRJNA669626
            BioSample: SAMN16483164
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Both,A., Kruse,F., Mirwald,N., Franke,G., Christner,M., Huang,J.,
            Hansen,J.L., Kroger,N., Berneking,L., Lellek,H., Aepfelbacher,M. and
            Rohde,H.
  TITLE     Population dynamics in colonizing vancomycin-resistant Enterococcus
            faecium isolated from immunosuppressed patients
  JOURNAL   J Glob Antimicrob Resist 28, 267-273 (2022)
   PUBMED   35134550
REFERENCE   2  (bases 1 to 4305)
  AUTHORS   Both,A., Kruse,F., Christner,M., Huang,J., Mirwald,N., Franke,G. and
            Rohde,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) Institute of Medical Microbiology, Virology
            and Hygiene, University Medical Center Hamburg-Eppendorf,
            Martinistr. 52, Hamburg, Hamburg 20246, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Trycycler v. v0.3.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 510.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/27/2020 16:36:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.13
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 3,265
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,071
            CDSs (with protein)               :: 3,071
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 32 of 103
            Pseudo Genes (incomplete)         :: 65 of 103
            Pseudo Genes (internal stop)      :: 16 of 103
            Pseudo Genes (multiple problems)  :: 9 of 103
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="BMT-1-1-10"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pIN-1-10_06"
                     /geo_loc_name="Germany: Hamburg"
                     /lat_lon="53.33 N 10.1 E"
                     /collection_date="2014-07-08"
                     /collected_by="Institute of Medical Microbiology, Virology
                     and Hygiene, University Medical Center Hamburg-Eppendorf"
     gene            complement(91..1497)
                     /locus_tag="IQN16_16305"
     CDS             complement(91..1497)
                     /locus_tag="IQN16_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="UOC33613.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDENI
                     YLEQINIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKMEPQREFIVQIGTL
                     DDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPSLSVYNAVIHNDEVSP
                     HLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIERLMA
                     DFGWERELVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYICRQREENEREK
                     ALVASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDFVDLYQR
                     ATRATNAKRNADNMSEQYLQMLEQNTELNEYVAALEEERHLSGRKQLADLQVENKKLKE
                     ENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFYRQNVSQHK
                     LERSEDEIEW"
                     /name="plasmid recombination protein"
     gene            complement(1719..2081)
                     /locus_tag="IQN16_16310"
     CDS             complement(1719..2081)
                     /locus_tag="IQN16_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOC33611.1"
                     /translation="MGMTLADIKVQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKRF
                     GFESFADFERWLSSISDDQQHEKSVGNELRADEKSAPSERNYPNESDGIFGGIPNRKSQ
                     MSESGFASMFSDVNSR"
                     /name="hypothetical protein"
     gene            complement(2085..2423)
                     /locus_tag="IQN16_16315"
     CDS             complement(2085..2423)
                     /locus_tag="IQN16_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOC33612.1"
                     /translation="MVAILEVRCDMDKIDLREETVNRIEVMYERLADYLNLSDEDNYSE
                     MLERMMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDEAEKEFWLKLMSHIMQLNQISV
                     INSKEKGM"
                     /name="hypothetical protein"
     gene            complement(2402..2821)
                     /locus_tag="IQN16_16320"
     CDS             complement(2402..2821)
                     /locus_tag="IQN16_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOC33609.1"
                     /translation="MTDSTDKMTIKALADELGITKNTVRNYLVKAGYDLARYKKNGIIL
                     LDKELIGVARSIYRSKGGQLTGHLPVNSTGSDFPLEKENEFLRDQLAEKDKHISELTEM
                     IRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
                     /name="hypothetical protein"
     gene            complement(2818..3807)
                     /locus_tag="IQN16_16325"
     CDS             complement(2818..3807)
                     /locus_tag="IQN16_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="UOC33610.1"
                     /translation="MKKKKQDLINGTFREKKKNLKKVVAFSDNRISPKMRELMTGCGSY
                     LEFIATIDKEKKKLVQAHFCKNRFCPLCAWRKARKDSMMLSIMMQAIAEEKQYQFIFMT
                     LTTPNVKAECLNDEIDLFNHALKKMFMRKRVKTSIKGYVRKLEITYNKKRNDYNPHFHL
                     ILAVNKSYFTDKDYYIKQSEWLDLWREVTGKTGINPDGTDEITQLDIRKVKGFQQEKAV
                     QEVAKYSAKDFELTESQEVFDTFYHAMKGRQLITFNGVFKEYKKKYESGDLDRYKKKDE
                     NEYFWFLTASWMRKESKYAVDYREMSADERNYFSGMYVDEKSVEGENE"
                     /name="protein rep"
     Typing          complement(95..1497)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.359"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 caaatgtcag cttaataggc tagtggttta gccgtcaact ccttatcctc ggcacaaacc
       61 gcctccggta ccgttgactg cacatcgtaa ctaccactcg atctcatctt cagaacgctc
      121 taatttatgc tgtgagacgt tttgacgata aaataaggtt ttctcctgct ctaatacttc
      181 taattcctca gaaagcccca tatccttttc tgcaagccca aaacggatag acaaacgagt
      241 gatcgatttt tgaaatgcgt tttttaatcg ctctaaacgc tggcgtaaag agcgattttc
      301 ttctttcagc tttttatttt ccacttgtaa atctgctaat tgttttctac cagacaaatg
      361 acgctcttct tccaatgcag ctacatactc atttaattct gtattttgct caagcatctg
      421 taaatattgc tcactcatgt tatcagcatt tctcttggca tttgttgctc tcgtagctcg
      481 ttgatacaaa tcaacaaaat cattcccaga taaatgatac gaaccatcag gaagcctctt
      541 gaaactttct aaaggaacca tatctttttg gaaaatgacc gccatctctt tttttacatt
      601 cagcatattt ttcttctctt cttcaatctt cgctctctca gaagccacca gagccttctc
      661 acgctcgttt tcttctcttt gcctacaaat atacctcaac tcatcagaaa cgctctcacg
      721 ctcttttacg gccgtttgac gcaattcgga aatttcgctc atgatttctt tgtattcatg
      781 aatatctttg attttgttag taccgactaa ctcacgctcc caaccaaaat ctgccatcaa
      841 acgttcaatt gactcaactt cctgattcct aaaattattc caaatcactc gaccattctc
      901 tttatctgct cgtaaaccct gctgttgcaa ggctttgtta aaacttggtt gcttttgaac
      961 accccgttta tacccttcag caacaggaac aatattcaaa tgcaggtgcg gacttacttc
     1021 gtcattatga ataacagcat tgtagacgga taaactagga ttccgttctt taaattcttt
     1081 gaaatactca actaaaattt tgttggcaat cactcgattt tgttctgctt gttgttccga
     1141 tatgccagta gaagaaccat catcacgagt agtacgaaaa tcatcaagag taccaatttg
     1201 cacgataaat tctcgctgtg gttccatttt tttatccttg cgaacctttg ctaaatagtc
     1261 ctcaattttt ctatcggctc ttttctgctt tgcattatat ttttctacgg cttcaccgaa
     1321 taattcttca tatttttcac gaatatttat ttgttccaaa tagatatttt catccgattt
     1381 tgtgaaatca atatgcttat gccagtcatt gattttttgg ctatcagtta actcacgatt
     1441 attatgagaa atgcttgtat tctttgttcc ctttttgaac gaaatactaa accccaagtt
     1501 aaacccctcc ttcgcgctgt cgctaataaa aagagtgtaa acctttctgt tactctttgt
     1561 caaagagtaa cgttttccta attgtgacat gactgtcaca attccaaacg ctctccgagg
     1621 gttttcgccg tcacaagtga caacgaaaaa aggagccgaa agcgtgtcac gctctcaact
     1681 ccttttacgc tttattttgc ttcatgcaaa atattcgctt atcgtgaatt tacgtcacta
     1741 aacattgacg caaatccact ctcactcatc tgactttttc tattcggtat ccctccgaat
     1801 attccgtcag attcgttagg gtaattccgt tcgcttggtg ctgatttttc atcagctcgc
     1861 aactcattac ccactgattt ttcgtgttgc tgatcgtcag aaatcgacga tagccaccgc
     1921 tcaaaatcag caaaagattc aaacccaaaa cgcttgcgaa gagccttgcc cattgcagca
     1981 ttttctttct caatcaatcg tttcttctgt tggcgaagtt tcgcctgttc tttttttaat
     2041 ttttcttctt gcactttaat atctgctaaa gtcattccca taaactacat ccccttttct
     2101 ttggaattaa taacggaaat ttggtttaac tgcataatat gactcattaa tttcaaccaa
     2161 aattcttttt cagcttcatc agttgtttgc gctaaatgct tatgaacaaa ttcattagtc
     2221 aaaactgaag attttgctag atcagcattg ataaccgagt tgagaacatc catcattcgt
     2281 tcaagcatct cactataatt atcttcatcc gaaagattca aataatcagc taaccgttca
     2341 tacatcactt caattctatt gactgtttct tctctcaaat caattttatc catgtcacat
     2401 ctcacttcca gaattgccac cattttcgat tttttaattc ttgattttct agtaacaact
     2461 gatcgttttt ttgttgctga agcagggtca atttttgttg atcacgaatc atttctgtta
     2521 attcagaaat atgtttgtcc ttctcagcta attgatcacg caaaaactca ttctcttttt
     2581 ccaaaggaaa atcactgccc gtgctattga ccggtaaatg accagtcaat tgaccacctt
     2641 ttgaccgata aattgaccga gcaacaccaa tcaactcttt gtctagcaag ataattccat
     2701 ttttcttgta ccgagcaaga tcatatccgg ctttgactag ataatttctc actgtatttt
     2761 tggttattcc tagttcatca gcaagcgcct taattgtcat tttatccgtt gaatcagtca
     2821 ttcattttcc ccctcaacag atttttcatc gacatacatt cctgaaaaat aatttctttc
     2881 atcagcactc atctcacgat aatcaaccgc atatttcgat tctttcctca tccacgaagc
     2941 agtcaaaaac caaaaatatt cattctcatc ttttttctta taacgatcaa ggtcacccga
     3001 ctcatatttt ttcttgtatt ctttgaatac accgttaaac gtaatcaact gacgaccttt
     3061 cattgcatga tagaatgtgt caaacacttc ctgactttcg gtcagttcaa aatcttttgc
     3121 cgaatatttc gcaacttcct gtactgcttt ttcttgttgg aaccctttga ccttgcgaat
     3181 atccaactgg gtaatttcgt cggtaccatc cggattaatt cccgtttttc cggtcacttc
     3241 ccgccacaaa tctaaccatt ccgattgctt gatataataa tctttatccg tgaaataaga
     3301 tttatttact gccaaaatca agtgaaaatg cgggttatag tcattccgtt ttttgttgta
     3361 cgtgatttcc aattttcgca catagccttt aatcgaagtc ttaacccgtt tgcgcataaa
     3421 catctttttc aatgcatgat taaacaaatc aatttcatcg ttcaaacatt ccgcttttac
     3481 attcggtgtc gtcaacgtca taaaaataaa ttgatactgc ttttcttccg caatagcttg
     3541 catcataatc gacaacatca tcgaatcctt acgagcttta cgccaagcac ataacggaca
     3601 gaaacgattt ttacaaaagt gagcttggac caatttcttt ttctccttat caatcgttgc
     3661 aataaattct aaatatgaac cacagcctgt catcaattca cgcattttgg gagaaattcg
     3721 attatcacta aatgccacca cttttttcaa atttttcttt ttttctcgaa atgttccgtt
     3781 aatcaaatct tgctttttct ttttcatctt gctatactga atctacaaat tttgtataca
     3841 aaaaaggctg aaaagccgat aacaaaaaat agattgctct cctttctagt ttagtttttt
     3901 tctagtcaat taaaactata aaatactagg gaagaaaaag caatcttttt ttttgttctt
     3961 ccttagagcc acaagggata gacccatact atagccccga tttcttcgca gaaatagttg
     4021 cctatagtat caagataaga aaaaacatca aaaaagaggg cgaaaatcgc cccctttttt
     4081 tcgttttaaa accccaaaac ccaaagacat aaaattcgat caatcgacca ctgcattttt
     4141 aagggggtgt acatattttg tacatttaac cgcctgttag atagggctgt taaattttcc
     4201 cactcattct tagtcatgca aacataaaac cgcacccttt aaaaagggcg cccccaaact
     4261 tcgctaacgc taagttggcc aaaaaatgga cttgccattt tttag
//
