LOCUS       CP063560                7165 bp    DNA     circular BCT 04-APR-2022
DEFINITION  Enterococcus faecium strain BMT-1-1-10 plasmid pIN-1-10_05, complete
            sequence.
ACCESSION   CP063560
VERSION     CP063560.1
DBLINK      BioProject: PRJNA669626
            BioSample: SAMN16483164
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7165)
  AUTHORS   Both,A., Kruse,F., Mirwald,N., Franke,G., Christner,M., Huang,J.,
            Hansen,J.L., Kroger,N., Berneking,L., Lellek,H., Aepfelbacher,M. and
            Rohde,H.
  TITLE     Population dynamics in colonizing vancomycin-resistant Enterococcus
            faecium isolated from immunosuppressed patients
  JOURNAL   J Glob Antimicrob Resist 28, 267-273 (2022)
   PUBMED   35134550
REFERENCE   2  (bases 1 to 7165)
  AUTHORS   Both,A., Kruse,F., Christner,M., Huang,J., Mirwald,N., Franke,G. and
            Rohde,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) Institute of Medical Microbiology, Virology
            and Hygiene, University Medical Center Hamburg-Eppendorf,
            Martinistr. 52, Hamburg, Hamburg 20246, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Trycycler v. v0.3.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 510.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/27/2020 16:36:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.13
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 3,265
            CDSs (total)                      :: 3,174
            Genes (coding)                    :: 3,071
            CDSs (with protein)               :: 3,071
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 32 of 103
            Pseudo Genes (incomplete)         :: 65 of 103
            Pseudo Genes (internal stop)      :: 16 of 103
            Pseudo Genes (multiple problems)  :: 9 of 103
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="BMT-1-1-10"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pIN-1-10_05"
                     /geo_loc_name="Germany: Hamburg"
                     /lat_lon="53.33 N 10.1 E"
                     /collection_date="2014-07-08"
                     /collected_by="Institute of Medical Microbiology, Virology
                     and Hygiene, University Medical Center Hamburg-Eppendorf"
     gene            join(6817..7165,1..32)
                     /locus_tag="IQN16_16260"
     CDS             join(6817..7165,1..32)
                     /locus_tag="IQN16_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UOC33602.1"
                     /translation="MSEQNQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            14..928
                     /locus_tag="IQN16_16265"
     CDS             14..928
                     /locus_tag="IQN16_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="UOC33603.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1168..1857
                     /locus_tag="IQN16_16270"
     CDS             1168..1857
                     /locus_tag="IQN16_16270"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOC33604.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTTCY
                     KEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYENLFQQATNMQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(1978..2064)
                     /locus_tag="IQN16_16275"
     CDS             complement(1978..2064)
                     /locus_tag="IQN16_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017548328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="UOC33607.1"
                     /translation="MFEQFICPLLVGVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            2709..3446
                     /locus_tag="IQN16_16280"
     CDS             2709..3446
                     /locus_tag="IQN16_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UOC33605.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEIF
                     TFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDKVN
                     ETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKGHWF
                     VKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAYKGNK
                     IGSLQIYFEENIPSITLDNWLEK"
                     /name="replication initiation protein"
     gene            3475..3996
                     /locus_tag="IQN16_16285"
     CDS             3475..3996
                     /locus_tag="IQN16_16285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="UOC33606.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKVV
                     LRSGVLQLASNLGNQRLLDRLHEYEQENDKNVYISRKNVVVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEEPEEEVLTSVNKENNNAI
                     TTEKKWWKFW"
                     /name="DUF536 domain-containing protein"
     gene            4080..4384
                     /locus_tag="IQN16_16290"
                     /pseudo=""
     CDS             4080..4384
                     /locus_tag="IQN16_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /name="helix-turn-helix transcriptional regulator"
     gene            4943..6097
                     /locus_tag="IQN16_16295"
     CDS             4943..6097
                     /locus_tag="IQN16_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="UOC33601.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQPI
                     EEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDA
                     DNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIK
                     LLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDNFIMI
                     NWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKSLNQDQ
                     IIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQ
                     GGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
                     /name="peptidase C47"
     gene            6091..6513
                     /locus_tag="IQN16_16300"
     CDS             6091..6513
                     /locus_tag="IQN16_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOC33608.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDHS
                     ERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNKKQ
                     LDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
                     /name="hypothetical protein"
     Typing          2710..3446
                     /name="rep_cluster_1742"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          15..925
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agctacaaaa gatatggcaa cagttaaagt aagccgaacc acttcttgta gtcgtgcaat
       61 taattacgct gaaccacgag caaccattaa aacaggaata aactgtgacg tcgattatgc
      121 caaaagtgaa atgaaacaaa ttcgcatgct gtatggaaaa gacgaccgtg tacaggcaca
      181 cttattgatt caatcttttc gaccaggaga aattaccgct gaaaaagcta atcaactagg
      241 ggcagagtat gcaaaaaaaa tagcccccga tcaccaaata gctatttaca cacatacaga
      301 caaagaccat attcataatc acattgtcat taactccgtt aatctggaga ctggaaaaaa
      361 atttcaagct catggtcaag catttctaaa caaatgttat gacctaaatg acgagatttg
      421 tcttgcacat aacttaccaa tcactgagcg aggaaagaaa ccagaaaaac gaaccatgtc
      481 tgaaataaaa ttaaaagaaa aaaatgaacc tgtttggaaa gacgaaattc ggattgcgat
      541 cgatcaaaca atgaaaaaca aaaaaaccag aacctatgac caattttgta attccctcaa
      601 aacattgggg attcattgct tcaatcgcgg gaaaaatttc acttacgaat tgatccacaa
      661 aaagaaaaaa gtacgatcca acagactagg aaaggactac gaaaaggaga cgattttaca
      721 tgagctggac agacgagaac gaacctataa ccaacagcga accaacgagc tacgaagagc
      781 tcgagctagc attcgcgaaa ctgcagaaga agtacgaaga gaacaacgaa ctcttacgcc
      841 tgaacaaccg gaaattagac cggctcgaag aaaccatgaa caacacgttg aaagaggaac
      901 agaccaccaa caagaattat cgctctaaaa cgttaaaaga catagaaaac gaaataaaga
      961 aacaacgtgc ttttctcgtt ccttatacgc aagaaaggat acaaactacg attgatttac
     1021 aagttaaaaa actccatcaa gagctattag accagcgaaa taaggaacag aaaaaggtgc
     1081 ttattcaacg atcattggat tttcttgccc ccttatgttg cttagtcctg acactgttcg
     1141 cattttggtt agtatgggga ggctaaaatg gcattttatt taccctatct actcattttt
     1201 gttagtattt caggaagtat ttggctcatc tataaaattt ttcaaacaag atattcatta
     1261 aaaggctcta aaattcgatt taagcgattt ttctttcttg gctgtatatt ttccttgata
     1321 atcgtttcaa gcggcttatt cggcgtttta gagggaaata agcgcgtttc tagaagtatt
     1381 ttattaggaa atgtcactca aaagtatgaa tcagcgcgtg ataaaaagaa aaaagagcaa
     1441 gcattagcac aaaaaatgga agagtttacc acttgttata aggaaatgaa cgatattttt
     1501 gtgaatcaag aaaaacgttt gacggataaa aacatggaaa aactcactcg tctttatcaa
     1561 aacttgccag aaaaacagca aaaagaggtt caagacaact atgagcgaac caaaaaagac
     1621 gtgcaatatg taaaagatac aaagatagaa gaagcctgta gtgatctatt tggagacaca
     1681 aacccttggt ttgccagtga agaagaaaaa aaagaaaaac aacaatctgt cacatatgaa
     1741 cgctacgaaa acctgtttca acaagcaact aacatgcaaa atccgacaaa aaaagaaacc
     1801 gccttaaact atctaaaaag tgtcaaggaa tggttagatc agcaacagca aaattgaatt
     1861 tgattgaaac tcatgttaaa ctgaatgtgc aaatgatcaa acattagata cacgaaagcc
     1921 ccagagaggt cggattctct ggggcttttt ttcaggttga tgtcgccttt actgatttta
     1981 cttcttgttt cggttattca accaataact gaataaagag acacaaactc ctacaagaag
     2041 ggggcaaatg aactgttcaa acattagata cacctccttc cagtcgcaag actgtcggat
     2101 aggcgacaag ttattatacc ctacaaaatt aaatctgtgt ggcgatttta agtatacctt
     2161 attttcgggt tgttagggct tgccctgacc gcctgtaagg cgcttctcga ctagattaag
     2221 ggagttaatc aagaattgtc ttagaacggc ttagacaagc tctcagattc tcccacctcc
     2281 tctttgaaca acggttttag caaaggagcc ggaggcgact gcgcaggtac aatgtgagag
     2341 cgtagcgatc acaccttttt agcgaccgta gggagcaagt tttctagttt agaaaaattc
     2401 gcgaaccttt ttggaggtaa aagtaggagt tttttaggcg ggaaattaaa tgtgctctta
     2461 agatatactg ttagatatac tgttagatat actgttaata ataactgtta gggattagca
     2521 accttatggg agagtaagta ttgacatagg tagtggggga taaattgtca cactatgggg
     2581 gataaattgt cacactatgg gggataaatt gtcacgctat gggggataaa tcgtcacact
     2641 atgggggata aattgtattt tacaccctct tatgtctatg ataacataat ttattgaggt
     2701 gatttttatt gagtaacgat atagttcgat ataataatgg attaaattct gtccctttaa
     2761 gaaaatttac ccctatagat atggatttat tttggggaat atgttctaaa atgaaaagaa
     2821 aaggtacaaa agaagaaatt tttacattta aacaaataaa agaattaatt gattacaata
     2881 taaatgcacg tggcgaccgt tttgctaatg atttaataaa aatgtcaaaa aaaatcatta
     2941 ctttgtcttt tatttatgaa gacgaaaaaa catatgaaca attaaatctg tttcaaaaat
     3001 ttaaaatcga taaagtgaat gaaacattat taataaaggt ttctgaagaa tttgaatata
     3061 tacttaattc aatcggaact aattttactc gtttcgaatt agaaaatatg acaaacttaa
     3121 agagtagcta cgttaaagag ttatatcgcc aacttatggc tcataaagac agaagatcta
     3181 aaaaaggtca ctggtttgta aaaatagaag attttagagc tctattagca ataccagaaa
     3241 gttatcgcat gagagatatt gataaacaaa tttttgccca agcgaaaaaa gaatttttaa
     3301 acgttaattc aactcaccct gctattttta aaacttttga aatcaaaaaa attaaagcct
     3361 acaaaggaaa taagatagga tctctgcaaa tttattttga agaaaatata ccttcaatta
     3421 ccttagataa ttggttagag aaataataac ctaacaattt aggaggcaat tatcatgcaa
     3481 cccaaagaaa cctttacaat aaaggaatta tctgaattat ttgaaataag taggcaagca
     3541 atttctaaac atgttgataa attagacccg tccttattag ctaaaaatga gcgtggttat
     3601 aaggttgtat tacgttctgg agtattgcaa cttgctagca acttaggtaa ccaaaggtta
     3661 ctagataggt tgcatgagta tgaacaagaa aacgataaaa acgtttatat atcaagaaaa
     3721 aacgtggttg taaacgagtt gctagataag ttgcgtgaag aaaatgaatt tttacgagaa
     3781 caactttctc tcaaagagca aaataattct aatatgcaaa aattactcga ccagcaacaa
     3841 caactcacct tgcaggcaaa tcaacagatc gaaaaactgc aagaacagtt acaattagct
     3901 tatacggaag aacctgaaga agaggtatta acttctgtca ataaagaaaa caacaatgca
     3961 ataacaacag aaaaaaagtg gtggaaattt tggtaacaat taccaacttt ccataaagaa
     4021 gaaatcaatt tctatagact ttatctttct aaaaataaaa aatatgttta ggtgatcaaa
     4081 tgactataag taaagtaatc aaacaaaaaa gaatagaaaa acaactaact caagaagatt
     4141 tagctgaaat gcttttagta tctaaaaaaa ccatttctaa ttgggaaaat ggtagaacaa
     4201 taccagatac agaaaattta attcaatttg ctaagttatt tgatttatca ctagacaata
     4261 ttttgttagg agaagaaaaa gttatgttaa aaaatcatac aaaaacacta atactattat
     4321 ttcattttgt aattttttta agtttagttt ttaccttaat tcagtccaaa ttcttttatt
     4381 ttagccattc aattgccact tggttattaa tattttttct aagtttgcta ttagtaatca
     4441 atactctgat tattattttt aataaacata aaaatttatt tcttcatagt gtttttccaa
     4501 ttattttgct gagtttttta tactttgtat aaagtataaa aaaagactgc tctttcgagc
     4561 agccagttaa aaaaataatg agagtttatt cttgaaaaag caacgcgctt ataaagcgct
     4621 accaaaattt tatcatattt tataaaaaat aacaatccag taaaaaatga ctggtaaaaa
     4681 ttcacaaaga aacggaaaga agcaactgca gaaaatcaag ccttaaaaaa agcttttgac
     4741 ctagaccaat gaaaaatcac aaagtgagca gctatagccc ttgatagagg ggctacttgg
     4801 catagtcgac attatgttaa ctaacgctat aaataacgat tatgttccca aaacaatatt
     4861 ttatgaacta taacaaaaat ttgttatcgc atattgtata ttttaattat aggtgtatac
     4921 cacttaaatt taacacaaag gcgtgattat tttgaaaccc aaaaagaaaa tttatctagg
     4981 tctcctcttt tccttgatga ttggaatttt tagtatagga attacaaata cctatgcttc
     5041 agaaaattct aatttcaata tcaactccca accaattgaa gaacaagcaa ttaattttgc
     5101 gaaaactgat tttgaacact ccataacttc aattctttta atggaaaata acaccgaaac
     5161 tgatattaac tatactcttg gttcgccatt taaaatgctc gatggagaaa atacggcaaa
     5221 tagttctatt ttctttccag tattttcaga cgctgataat acgattaaat atatttatac
     5281 cgtcaaaaaa aatacaaatg gagaatattc aggaagtatt tcacaatttt tagcaaaaga
     5341 acttcaaaat ctagtagata gtaatataac agatacaacc ttattttttc aagataatag
     5401 catttattat tccgataatg gacaaattaa actactttgg aaatcacctt taagtacaga
     5461 aaaaaatggt gtttcttcct ttacagaaaa aaatgatctt atatccgtaa atataaaaaa
     5521 taaaattaaa aatttctcta aaaaacaaaa ttttcctgcg gttaaacgtg taaaaagtgt
     5581 ctcctccgga gataacttta ttatgattaa ttggcgtata gcagaaaccc aaacaagttt
     5641 tccgtggtgt gctgcttacg tagaagctgc tatcttatgt aacaaaacag atgtacgtgc
     5701 tacttcagct agagccatta tgcattatac ctatcctaat ttaaacaaca gtcaaattga
     5761 atcaaaaagt cttaatcaag atcaaataat aaaatatgca aattactgtg gtgtttatcc
     5821 taaaaaagta gcacgaatac taaacctttc agaggtccaa acacaactaa gaaaaggaaa
     5881 tgccatttat atgggaactg ctggttatgg aaattatagt aacagccgtc atgcacttgc
     5941 attatttggg tgggtcaatc aaggtggtta tcaaacttac tatgtgtgga atccttggta
     6001 taactaccca actgtaataa gtgcaaatgc aggaccagtt acaattcctg tacctggagg
     6061 tggttttact tgggacaata ccattacaaa ttggtaaata aacccattgc tttagttatg
     6121 ggtctagtgt tagttctttt cttaactagt tgttcaaaaa aacaagaaat acaaaagact
     6181 gatctgattc cttttctagg aacttggcaa actaaagatc attcagaacg tctagtaatc
     6241 actaatttaa atacttcaaa aaatatagaa caatatacca tcttagtaaa agagaacaat
     6301 cataaaattc aaatcattga taaaaattta aatataaatg aacaagtttt catttacaaa
     6361 gcaacaaata atttactaac actgcgctct aataaaaaac aattagatta cacttttagt
     6421 ttggattctc caaaaaagct cgtatttatt ttgacaacga atgatgggac agaagggaca
     6481 gctaaaccaa ttgaattttt taaagctgat tgatgaatag ttgtttgata ttacaaaaca
     6541 ataatttcac cgaaggtgaa acaagcgaaa ctttacaaaa agtggctagc atttgctagc
     6601 cttttttgta ttgtggagcg tgggttttga gggggattca agggggagct tgcgcccctt
     6661 tgaaaagcgg ttatttgcta gccaaatacg taggtatttg gctagcaaat atagagcttg
     6721 ccaaaccctt ttagtgagct ttactcattg taccttttta ggtacaatgg tattgcgaac
     6781 gatacgtccg taggacgtac tgaaagggtg ggacttatga gcgaacaaaa tcaaaatttg
     6841 gctagcgatc attctaaaaa atatacctat cgatccgagc caaaacaaat cagttttcga
     6901 gtgagcgaat ctgaatttgc aaagctgaag cagtcagctg aagctttgca aatgagtgtg
     6961 ccggctttcg taaaagccaa ggcacaaaac gcgcgtctgg tgacaccgaa agtggcgcca
     7021 gacattgccc aggcaatcgc gcgcgattta gccaaagctg gagggaatat caatcaaatt
     7081 gctaagtggt gtaatacaca ccaacacgac gttgctcctg gagacgccca acgcttatcc
     7141 gaaaacctaa aaatcatgca aaagg
//
