LOCUS       CP063553               27222 bp    DNA     circular BCT 04-APR-2022
DEFINITION  Enterococcus faecium strain ICU-1-2 plasmid pZA1-2_04, complete
            sequence.
ACCESSION   CP063553
VERSION     CP063553.1
DBLINK      BioProject: PRJNA669626
            BioSample: SAMN16483165
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27222)
  AUTHORS   Both,A., Kruse,F., Mirwald,N., Franke,G., Christner,M., Huang,J.,
            Hansen,J.L., Kroger,N., Berneking,L., Lellek,H., Aepfelbacher,M. and
            Rohde,H.
  TITLE     Population dynamics in colonizing vancomycin-resistant Enterococcus
            faecium isolated from immunosuppressed patients
  JOURNAL   J Glob Antimicrob Resist 28, 267-273 (2022)
   PUBMED   35134550
REFERENCE   2  (bases 1 to 27222)
  AUTHORS   Both,A., Kruse,F., Christner,M., Huang,J., Mirwald,N., Franke,G. and
            Rohde,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) Institute of Medical Microbiology, Virology
            and Hygiene, University Medical Center Hamburg-Eppendorf,
            Martinistr. 52, Hamburg, Hamburg 20246, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Trycycler v. v0.3.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 261.8x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/27/2020 16:15:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.13
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 3,153
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 50 of 139
            Pseudo Genes (incomplete)         :: 75 of 139
            Pseudo Genes (internal stop)      :: 30 of 139
            Pseudo Genes (multiple problems)  :: 15 of 139
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..27222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="ICU-1-2"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pZA1-2_04"
                     /geo_loc_name="Germany: Hamburg"
                     /lat_lon="53.33 N 10.1 E"
                     /collection_date="2019-03-01"
                     /collected_by="Institute of Medical Microbiology, Virology
                     and Hygiene, University Medical Center Hamburg-Eppendorf"
     gene            complement(join(27059..27222,1..991))
                     /locus_tag="IQN17_15590"
     CDS             complement(join(27059..27222,1..991))
                     /locus_tag="IQN17_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="UOC30521.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGKL
                     GDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKYFD
                     EINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVMYLA
                     HDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRYNLQT
                     ITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFNNILKN
                     AAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSSDTGGAG
                     LGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
                     /name="VanA-type vancomycin resistance histidine kinase
                     VanS"
     gene            complement(969..1664)
                     /gene="vanR"
                     /locus_tag="IQN17_15595"
     CDS             complement(969..1664)
                     /gene="vanR"
                     /locus_tag="IQN17_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="UOC30522.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDKS
                     EIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITKPF
                     RPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPTEFS
                     ILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKYIKTV
                     WGVGYKIEK"
                     /name="vanR"
     gene            1853..2533
                     /locus_tag="IQN17_15600"
     CDS             1853..2533
                     /locus_tag="IQN17_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="UOC30523.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIGADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            complement(3149..4189)
                     /locus_tag="IQN17_15605"
     CDS             complement(3149..4189)
                     /locus_tag="IQN17_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="UOC30524.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLLK
                     DRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGRVN
                     KRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTTVIA
                     KIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVDIMKE
                     DSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELESYGIDA
                     DKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFNELPTVS
                     TENWLE"
                     /name="replication initiator protein A"
     gene            complement(4538..4864)
                     /locus_tag="IQN17_15610"
     CDS             complement(4538..4864)
                     /locus_tag="IQN17_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOC30525.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKDQ
                     GSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLAKK
                     QNKI"
                     /name="hypothetical protein"
     gene            complement(4851..5654)
                     /locus_tag="IQN17_15615"
     CDS             complement(4851..5654)
                     /locus_tag="IQN17_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="UOC30526.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDI
                     DPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFT
                     QVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLI
                     KLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRR
                     TITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
                     /name="ParA family protein"
     gene            complement(6007..6297)
                     /locus_tag="IQN17_15620"
     CDS             complement(6007..6297)
                     /locus_tag="IQN17_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="UOC30527.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITRQ
                     RRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
                     /name="type III secretion system protein PrgN"
     gene            complement(6433..6612)
                     /locus_tag="IQN17_15625"
     CDS             complement(6433..6612)
                     /locus_tag="IQN17_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOC30528.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAAA
                     GYHNFRFYIFPLYI"
                     /name="hypothetical protein"
     gene            complement(6609..6959)
                     /locus_tag="IQN17_15630"
     CDS             complement(6609..6959)
                     /locus_tag="IQN17_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOC30529.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTEE
                     IAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIRHI
                     EFYQDNKWSRLE"
                     /name="hypothetical protein"
     gene            complement(6952..8277)
                     /locus_tag="IQN17_15635"
     CDS             complement(6952..8277)
                     /locus_tag="IQN17_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="UOC30530.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRAD
                     NTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNIFR
                     RYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIATVG
                     VGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRLNQMG
                     IMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQVLPRD
                     YTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKIPITDNT
                     KELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEENLDKIIDK
                     IRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
                     /name="Y-family DNA polymerase"
     gene            8727..9341
                     /locus_tag="IQN17_15640"
     CDS             8727..9341
                     /locus_tag="IQN17_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="UOC30531.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRPV
                     FQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGNPL
                     LDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQRRL
                     VYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
                     /name="recombinase family protein"
     gene            complement(9506..10330)
                     /locus_tag="IQN17_15645"
     CDS             complement(9506..10330)
                     /locus_tag="IQN17_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOC30532.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELKT
                     LPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACEIG
                     VKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRNKLV
                     HSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQITLS
                     KELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
                     /name="hypothetical protein"
     gene            complement(10967..11224)
                     /locus_tag="IQN17_15650"
     CDS             complement(10967..11224)
                     /locus_tag="IQN17_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="UOC30533.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGK
                     PEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
                     /name="Txe/YoeB family addiction module toxin"
     gene            complement(11217..11486)
                     /locus_tag="IQN17_15655"
     CDS             complement(11217..11486)
                     /locus_tag="IQN17_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="UOC30534.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRD
                     YDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
                     /name="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
     gene            complement(11617..11796)
                     /locus_tag="IQN17_15660"
     CDS             complement(11617..11796)
                     /locus_tag="IQN17_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOC30535.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLLI
                     AQEKAGFEVPALKK"
                     /name="hypothetical protein"
     gene            complement(12177..12743)
                     /locus_tag="IQN17_15665"
                     /pseudo=""
     CDS             complement(12177..12743)
                     /locus_tag="IQN17_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            12819..13499
                     /locus_tag="IQN17_15670"
     CDS             12819..13499
                     /locus_tag="IQN17_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="UOC30536.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            complement(13555..15000)
                     /locus_tag="IQN17_15675"
                     /pseudo=""
     CDS             complement(13555..15000)
                     /locus_tag="IQN17_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M"
                     /name="type I restriction-modification system subunit M"
     gene            complement(15338..16726)
                     /locus_tag="IQN17_15680"
     CDS             complement(15338..16726)
                     /locus_tag="IQN17_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="UOC30510.1"
                     /translation="MYKERDDLNYTLIINKDGNYSWRPLVLLHPILYVDLVNCLTKEDH
                     WEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILNWWEYFEQKSLAQNIKYQY
                     CMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLGDKIDKKISGMQHNQTNGIP
                     QGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRYRDDYRIFSNNRNDLEQIMKV
                     LSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEIKASIRMNATNLDGDREIRYSI
                     NLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVDIMINNP
                     GSLPQCVLIIGQLLNFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTIFGEMFDS
                     VLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEGTIKDEEKYIKMSKKIYITEFDDFN
                     DYPF"
                     /name="RNA-directed DNA polymerase"
     gene            complement(16790..17743)
                     /locus_tag="IQN17_15685"
     CDS             complement(16790..17743)
                     /locus_tag="IQN17_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="UOC30511.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNFL
                     KCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFSCS
                     VSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNISERKKDYVVFEEEFGHL
                     EGDTIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQSVPKNLFKSITF
                     DCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRKDGLPKEMEFNQVNQGF
                     ISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
                     /name="IS30 family transposase"
     gene            18386..18949
                     /locus_tag="IQN17_15690"
     CDS             18386..18949
                     /locus_tag="IQN17_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="UOC30512.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAVR
                     PQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTPHG
                     RLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQVYS
                     VKEITEMTGISKATLYRYLKDNRE"
                     /name="recombinase family protein"
     gene            complement(19228..19515)
                     /locus_tag="IQN17_15695"
     CDS             complement(19228..19515)
                     /locus_tag="IQN17_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="UOC30513.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQK
                     TGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(19505..19834)
                     /locus_tag="IQN17_15700"
     CDS             complement(19505..19834)
                     /locus_tag="IQN17_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="UOC30514.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEPK
                     KEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEELA
                     AEIGL"
                     /name="AbrB family transcriptional regulator"
     gene            20139..20468
                     /locus_tag="IQN17_15705"
     CDS             20139..20468
                     /locus_tag="IQN17_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="UOC30515.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIKI
                     FYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDILS
                     AIRVF"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(20755..21240)
                     /gene="vanZ"
                     /locus_tag="IQN17_15710"
     CDS             complement(20755..21240)
                     /gene="vanZ"
                     /locus_tag="IQN17_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="UOC30516.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTPF
                     TATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGATD
                     ITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINYV"
                     /name="vanZ"
     gene            complement(21393..22304)
                     /gene="vanY"
                     /locus_tag="IQN17_15715"
     CDS             complement(21393..22304)
                     /gene="vanY"
                     /locus_tag="IQN17_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="UOC30517.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHLE
                     NSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLLDS
                     NIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGYSEH
                     NSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIRYVGL
                     PHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNLRYEIS
                     GNNIDGVIVTVFPGSTHTNSRR"
                     /name="vanY"
     gene            complement(22732..23340)
                     /locus_tag="IQN17_15720"
     CDS             complement(22732..23340)
                     /locus_tag="IQN17_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="UOC30518.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTYE
                     LAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRTEM
                     ISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEAQNR
                     RRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
                     /name="D-Ala-D-Ala dipeptidase VanX-A"
     ARG             complement(23347..24395)
                     /name="vanA"
                     /gene="vanA"
                     /gene_name="glycopeptide resistance gene cluster; Van
                     ligase"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="99.71"
                     /coverage="101.75"
     ARG             complement(23350..24377)
                     /name="vanA"
                     /gene="vanA"
                     /gene_name="D-alanine--(R)-lactate ligase VanA"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="99.71"
                     /coverage="100.0"
     gene            complement(24370..25338)
                     /locus_tag="IQN17_15730"
     CDS             complement(24370..25338)
                     /locus_tag="IQN17_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="UOC30520.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKSA
                     PFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAYSP
                     DSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKAVIE
                     RLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQRMKQ
                     GAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQFLLKL
                     QRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
                     /name="D-lactate dehydrogenase VanH-A"
     gene            25670..26961
                     /locus_tag="IQN17_15735"
                     /pseudo=""
     CDS             25670..26961
                     /locus_tag="IQN17_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /name="ISL3 family transposase"
     ARG             complement(24374..25338)
                     /name="vanH-A"
                     /gene="vanH-A"
                     /gene_name="D-lactate dehydrogenase VanH-A"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(20759..21240)
                     /name="vanZ-A"
                     /gene="vanZ-A"
                     /gene_name="glycopeptide resistance protein VanZ-A"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(973..1664)
                     /name="vanR-A"
                     /gene="vanR-A"
                     /gene_name="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(21397..22304)
                     /name="vanY-A"
                     /gene="vanY-A"
                     /gene_name="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="99.67"
                     /coverage="100.0"
     ARG             complement(22736..23340)
                     /name="vanX-A"
                     /gene="vanX-A"
                     /gene_name="D-Ala-D-Ala dipeptidase VanX-A"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(24371..25338)
                     /name="vanH gene in vanA cluster"
                     /gene="vanH gene in vanA cluster"
                     /gene_name="vanH; glycopeptide resistance gene cluster"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(970..1664)
                     /name="vanR gene in vanA cluster"
                     /gene="vanR gene in vanA cluster"
                     /gene_name="glycopeptide resistance gene cluster; vanR"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(21394..22304)
                     /name="vanY gene in vanA cluster"
                     /gene="vanY gene in vanA cluster"
                     /gene_name="vanY; glycopeptide resistance gene cluster"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="99.67"
                     /coverage="100.0"
     ARG             complement(20756..21240)
                     /name="vanZ gene in vanA cluster"
                     /gene="vanZ gene in vanA cluster"
                     /gene_name="vanZ; glycopeptide resistance gene cluster"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(27060..27220)
                     /name="vanS gene in vanA cluster"
                     /gene="vanS gene in vanA cluster"
                     /gene_name="vanS; glycopeptide resistance gene cluster"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="100.0"
                     /coverage="13.8"
     ARG             complement(3..931)
                     /name="vanS gene in vanA cluster"
                     /gene="vanS gene in vanA cluster"
                     /gene_name="vanS; glycopeptide resistance gene cluster"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="100.0"
                     /coverage="80.73"
     ARG             complement(22733..23340)
                     /name="vanX gene in vanA cluster"
                     /gene="vanX gene in vanA cluster"
                     /gene_name="vanX; glycopeptide resistance gene cluster"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="99.5"
                     /coverage="100.0"
     Typing          complement(3150..4189)
                     /name="rep_cluster_889"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cattgtccag cctatagaac ttttcaaata tggcagctag cttatctttt gggatgcttc
       61 cagtgttctt gaattcgatt gacaccacat ccccggagag gcccgcggta atgtcaatga
      121 tgctgttatc ctcactgtat gcagcggcgt ttttcaaaat gttgttaaag actctcgcga
      181 gtttatcagg gtcgccggac acggtcagat cctcgggggc gtgaataacc gcctgttttc
      241 catgtgcgga aagctgagga taaaattcat cggtcatctg caccagcata tagtataggt
      301 ctatgtgcgt ttttgttagc gttatcgttt gtaggttata ccgtgtaatc tcaaaaaact
      361 cgtcgattag ctgttcgagt cgatacgctt tgtccaacgt gatatgcaca tactttgcct
      421 tttgatctac cggcatgtct ggagcctcgt caagcaggct caaataaccg ataatggatg
      481 taaggggcgt tttaatatcg tgcgccaagt acataacaac gtcatttttt ctttgttcgg
      541 ccagctttgc atcctgctct cgcttttcca gagtccgttt taatgtgttg agcttttgtt
      601 ccataacatc catttccgca gaaagctcaa tttgtttatc ttcgttctga ataagtacat
      661 caatgccggt atttatctcg tcaaagtatt ttgcgaattt tgaaagcatg acgcgacata
      721 gaataagaat actaatgaca atcgccacat aaataaagat atctatattg ttccgtatgg
      781 aatattgata taatttcatc gcgtccaggt gatttaagtc atatttgttt tccaaaatac
      841 ttaagatcca atccccaagt ttccctcgga tcattgaacg aatatacaac acgaatacaa
      901 ttgctaccac aacaattgca acgatataca tgtaaagttt tcgttctagt ttggaatagt
      961 cgtttttttt atttttcaat tttataacca accccccata ccgtttttat atatttcgga
     1021 ttatcaatgg tgtcgttcat tttttcgcgc aaatgccgga tatgcacggt gatggtgttg
     1081 ttgctcttgc tgaaatattc gtcgccccat atctcatgaa atagcagctc ggagctaacc
     1141 acattcccct tgttttcaca gaggattcgc agtattgaaa actcggtggg agtaagggat
     1201 aactgcttct cgttcagata acactcatgg gtgttaacat taatgacaag gccggagtgg
     1261 acgataacat tttcgttctg ctcctttact ccactgaatt ttttgtatcg gcgcaactgg
     1321 gcctttaccc gagcaattaa ctccagtggg cgaaagggct tcgttatata atcatccgcg
     1381 ccgattgtta accctgtaat tttatctacc tctgtatctt tcccggtcag catgataatc
     1441 ggataggtgt gcttgtccct tattttttga cagatagtaa ggccgcttgt gccgggaagc
     1501 atgatgtcca atatggcaag gtcaatctca gacttgtcta tacattccaa tgcttctttg
     1561 gcggtatagt atttgaaaac cgtataattc tcgtttttta agtataattc aaccaaatcg
     1621 gcaatttcat gttcatcatc cacaataagt attttatcgc tcatagttat caccctttca
     1681 cataagtttg cccttaataa tttatcagat tatagggccg aatttataag catttacata
     1741 ataaatgact aagaatttct taagatgatg attcttttgg ttctgttgca aagttttaaa
     1801 tctactatca aataaggtag aataatagaa aaagatagca ggaggaatga cgatgaatca
     1861 ttttaaagga aagcaatttc agcaggatgt gattattgta gccgtgggct actatcttcg
     1921 ttataacctt agctatcgtg aagttcaaga aatcttatat gatcgtggca ttaacgtttc
     1981 tcatacgacg atttatcgtt gggtgcaaga atatggcaaa ctactctatc aaatttggaa
     2041 aaagaaaaat aaaaaatcct tttattcatg gaaaatggat gaaacgtaca tcaaaattaa
     2101 aggaaaatgg cattatttgt atcgagccat cggtgcagat ggtttaacct tggatatttg
     2161 gttacgtaaa aaacgggaca cacaagcagc ctatgctttt cttaagcggt tagtgaagca
     2221 gtttgatgaa ccgaaggttg tagtcacaga taaagccccc tctattacaa gtgcctttaa
     2281 gaaactaaaa gaatacggct tttatcaagg gacagaacat cgtaccatta aatacctgaa
     2341 taatttgatt gaacaagacc atcgtccagt aaagagacgc aataaattct atcgaagttt
     2401 acgcactgcc tctaccacga ttaaaggcat ggaagccatt cgaggattat ataagaaaac
     2461 ccgaaaagaa ggcactctct tcgggttttc ggtctgtact gaaatcaagg tattattggg
     2521 aatcccagct taaatcatag ataccgtaag ggattttatt ctttatttaa aactttgcaa
     2581 cagaaccgtc cgttgcgggt aataccgtaa cactgaaggg cacgcattaa ggcgtcatct
     2641 taatgctttc ctgtaggaca ctgacggaga ttaccagtct cccgagcctt acaggggcct
     2701 ttcttttttt tgtattttca attatcacaa tttatcgtcc aaaagtgcag aacgaaaatc
     2761 aattcatcac aagcatatca gacaattaaa tttaaaacaa ctaaaaatat acaaaaaagt
     2821 gtaattgaat attagcagca ctgcctgaaa agaagtaata tcaggctttt cagcttgggg
     2881 aaataagtga attacctctc ttcatgaatt attataactc aaaaaagcac atatgcaaac
     2941 actagttccc tatttttttg aatgaaaaat aactaaaata tctaacgatt atgactttaa
     3001 aacctacgtt tgtgattgtt ttcataaact tttaatctaa aaactccgga ataatagcta
     3061 tttatttgta acatttgtaa taaaattaaa atcataaaaa agttctaatt taaaattgat
     3121 catttttata gtgatcgctt tttatcaact actctagcca attttccgta ctaactgttg
     3181 gtaattcgtt aaatgtttct ctttgttcgt ttgcaagctt tgccgcaata gctttttcga
     3241 caaaccaatt ttttacataa acgaaaatta gattctgcaa attgtttact ttttcagttt
     3301 tttgtttttg ataagctttc agcaaagtca tatataatcc tttgtcagca tcaattccat
     3361 aagattccag ttcttcataa acaacaactg tttgctctaa ttcttcagct tttttctttg
     3421 cattatgaat agtttggacg gttttttgag cgtcttcgta agtactagaa aatgttttta
     3481 tcaagtttaa tacattttct ggtacaaatg tatttatact gtcctctttc atgatgtcga
     3541 cgaagttatc tagtaaaact tgattttgta actgatcagt ttcagtgtct attatgtgtc
     3601 tattagtgtc tatagaatta gaatagaata gattgtctgc gattttcgca attacggttg
     3661 agtccttgat atgactggat tttgccgtat ttgcgatttt cgcaattacg gtggtatcat
     3721 tgatttctcg cggttttgcc gtatttgcga ttttcgcaat tacggtggta tcagtattgt
     3781 cttcctcttc catgatttta aagacgtctt gattcgttac ttctggcttg cctaagtaca
     3841 ataaattagg catggattca atcttaccgt cacgtttatt tactcttcct ctcttctgtt
     3901 tcagaagacc tgctttttct aattcctttt tgattttaga tactttacct tcgcggcatt
     3961 gtaacaattt cattaattct tcaactgtga atatgaaaaa tatattatca tcagaatcta
     4021 cccagttatt tttgactgaa taatcaaatc gatctttaag tagcatataa gcaatttttg
     4081 catcatttga tagatccatg tatttttcgt tagtaaaaaa taccttaggc atttgataat
     4141 atttttcacg ataaatttct tttgatttgt aataattaaa atcattcata gccaatcctc
     4201 tttctgtagg atagcgtttt gttcaaaact atgttacaat gaattaaaca aatatatcta
     4261 tccactatat atttttctac tgctgataag tcgccaaact ttgttaaagc agtagtcttt
     4321 ttttatcttt ttttagttta ccttaaaaaa taaattaaag caaaccgctc tatttcaagg
     4381 tttaagctca tagtaactat atcacgatat tacaataata ttatatcacg atatcgtaat
     4441 attacaatat gaagataagc gacttatcag ctttgatact actgattttt ataaaaaaaa
     4501 tatcacgata ttatgatatc gtgatattga atgtaaatta aattttattt tgtttctttg
     4561 ctagtttata tccttcaatc aagtattctt caggtatgga ctttaatagc gcatcttcta
     4621 taacgtctgg tttacttttg tctgattcag caatagtcgt ccaatatttg agtgctgaca
     4681 ttagaattgg atttatacga atgctatctg ctgttcttac actacttcct tgatctttta
     4741 tgactttact tatatttttc ttggttaatt cgatttcttc tacttctttt gatttaaaat
     4801 tctttttatt tttatttcct agtaatctac ttactgcagc ttgactgcga ttatcttctg
     4861 ccatcaaaat caccaacttc ttttaaatat tcttctgtaa atgctttata gtcatcttca
     4921 gctgttgagg gtttattaaa attgattaga tctgatccag cagcttgcgc ttcgtcaacg
     4981 gttattgtcc tacgaatata agttttgaaa atttttgcac caatgactct agaaatttcg
     5041 gttgctgcag acgtcatttc tttagaaatg ttataacgct tattatactt agtaaataga
     5101 actcctcgta cttcaagctt gtcgttcgta tattctttta ccgtttcaat agtttcactt
     5161 aactttatta gtccttttaa tgagaaatac gcagcttcaa ctggaatgat aatttcatca
     5221 gaaacagtaa atgcgttgac tgtaagcatt cctaaacttg gtggacaatc aattataata
     5281 taatcatagt tgtccttgat tggttgaagt actcttttaa gcctgtgttc acttcctact
     5341 tgagtaaatt ccctctctgc agaagaaagg gctatgtcgg ctggcaaaac atcaattcct
     5401 ttatagtcca gaatagcatc tgtaatgtca gcctcccctt ttaatacttc ataaattgta
     5461 ggttggtttg ttccatctat atcgaaacct acattatctg tagcatttcc ctgaggatca
     5521 atgtccacta aaagtacatt tttaccgtaa acaaatttca aacatgctgc taaattaacg
     5581 ctagtcgtag ttttagaaac tccacctttt tgattggcta ctgacaatat tcttgccatt
     5641 aaaatatccc ccattcatct tatattataa tataattata tcataatatt gtgatattgc
     5701 aatatcgtat atgcattata ttgtaatatt gtgtgattat tttgttttaa agatatatat
     5761 cggttcggat ataaagatta ttcatatgtt attcgaaatt aaaataatca ctttttatta
     5821 ctttaaaatt ttttattaat gataagaaat atgttttgcg agcagcaact agaataattt
     5881 aaacattact aaactgtcta tattgttact aataattccc ttcttatagt gcattttctt
     5941 agaaaaaaac aatattacga tatcatgata ttaagatatc gtaatattat acaattgtga
     6001 ttgatattat atatgttttt ttattttttt atcatgttta atcttgaagg aatcatattc
     6061 tttttctaaa gacaggagct tttcataaac atcagacatg tttaaactag aggccaggct
     6121 taaatcataa acgaatgaag caggtaatga ttttattcta cgttgtcggg ttatccagct
     6181 tgcaaccgtt ccttgtgggt aggcatataa ctcacaaaat tgttctacag tgattcctaa
     6241 attcttgata atgtaaacat tgatcgggtg cgagtaaaca aatgtattga tagacatggt
     6301 gcgtaagcct actttctttt attattgcgt ttgcaataat agtttaacac acgcattatt
     6361 ctagtatcta ctcctatttg taaaaacaaa aaaggattca tagagataac tctatagaat
     6421 ccgtaaaata ccttatatat ataacgggaa gatataaaac ctaaaattat gataacctgc
     6481 tgcagcttct acatagaaag agaagcgctc aacaccaacc tcatttatca aacgttggcc
     6541 ataaccccaa atctcttctg atagctgatc aaaggttata tactttagct catattgctg
     6601 gataatgttc attctaatcg actccattta ttatcctggt aaaattcgat atgacgtatt
     6661 tcgtcgtagt gaattttgtc ttttccaacg tagatcccca gctcatcact acctaagatc
     6721 ttgccaatga tatcttctgg atagtatcct tcagcgtttc gttcttcttt ttgaatagat
     6781 actgatctat tttttatgac tgcagcgttc aatacttccg caatctcttc agtcgtcatt
     6841 tgttcttttg ctaaattttg actggttcgt tgttcattgt cggcatcaat ttcttcagta
     6901 tgatcagata aataaaaccc tgcccacttt aaacgtccac gatcattgta atcaatcatt
     6961 tttaattccc ccatttccgc ccgcatgacc accaacaagt gtgcttctag taatgcttct
     7021 agcgccttct aacattgaag aagcatgtac aatcgacgta aaaccatatt tttgccgaat
     7081 cttgtcaata attttatcta aattttcttc gtcaatttgc ttttgtggat cactaaataa
     7141 atccagctga agcgaatcgg tgtacacaag cttgctatag gttattccaa catgccttac
     7201 ttcttgtcct tcataatact tatcaaacaa gaaaagacaa taattctgca attcctttgt
     7261 attatctgtg atagggatct tcatctggtg tgaaaaccct ggtctcgttt ctaagatcga
     7321 cgtgccaatg tgtaaatgga cacaacctgt tttacagtta tgctgcctaa tgcgtatcgc
     7381 tacttgttcc gccatttctt ttaccaccag ctcgatttca tttcttctag tataatctcg
     7441 tggtaacact tgtgaatttc catatgattt ttcctttgtc ggttctggtg ggattgctat
     7501 atctgttcga tcgatcccat tagcatggaa gtacagctgt aagccaatca cacctagtct
     7561 attcttaatc gtataaggat tccagttagc aagatctcta atgctcataa tccccatctg
     7621 attgagtctt tttttcattc gagagccaat tccccaaaag tccgtcattt caggaatatt
     7681 ccatactttt tcaggaacgt tttcgtacgt ccattccgca acaaatcctt cattatgctt
     7741 tgcttcgttg tctaaggcta gtttagccaa aaggggatta tctccgacac ctactgtagc
     7801 aatcaatcct agctcttctt taatattctc ctggatcatt tgagccagct tctttctccg
     7861 ttgacttcgt gttccttcag tcgtaaaaag attcagtgat cgggtcactt ttaagattga
     7921 ttcatcgatc gagtaaatca gtagatcttc atcagccaca tatcttctga aaatattatt
     7981 tacctgcata tttcgcttga tatataggtt catacgtggt ggaacaacgt gtagtgtttt
     8041 aggaaatggt tgtggtaagt cacgtggtct actcacattt gtaataccat accgcttttt
     8101 tgcttcagga gaagaagcta atatcaatcc tgaaccagta ttatcagctc gactcataac
     8161 aacaagttct gttgttaatg gatctaaatt tctttctata cattcgacac ttgcgtaaaa
     8221 gcttttcata tcgattaaaa agtaatcatt tactggctct tttgaataat ccatcataaa
     8281 taacactcca ttctttcaca ttatacaaac atatgttcga tattgtaaag aagaaatagc
     8341 taaatacata aaaaaagaag cataggtatg ctatcaccct gcttcttctt ttgatttgta
     8401 tgatcaattt attctatcac tttactaatt aatttgaaaa tctaataaaa actctcactc
     8461 taaaagagtg agagactgca aattagagta cggacttttt tggagttccg ttctttaata
     8521 ttaacaaatg aataaaatcc ttacaacatt tgagaataag ttatcagtag acacttacaa
     8581 aaaattctca tactatttcc tccatcatta gtttttgtct atataaggtt taccctaaat
     8641 agacactggt ataacattga aaaatatggc ttcctcgtgt atgattaggg tgtattttaa
     8701 atagttgcag aaagtaggta aaagttttga gagttgcata catacgtgtc tcatcgatcg
     8761 atcaaaatga acaacgccag attgaagaaa tgaaaaaatt cggtgcagag agaattttca
     8821 tcgagaaaca atctggcgca acgattactc atcgacctgt tttccaagaa gcattagatt
     8881 acgtccgaga tcaagatatt tttattgtgg aagctattga tcgacttgga agaaattatg
     8941 acgagatcat cgcttcagtc aattacttga agaagaaaaa tgtgaagctg atcattacta
     9001 gtttaccgat catggctgaa gcaattggca atcctctcct ggataaattc attaaggatc
     9061 tgatcatcca aattttggcc atgatcgctg aacaagaacg gactgaatcg aaacgtcgcc
     9121 aggcacaagg aattaagatc gcaaaagcaa acggcgtcta caaagggcgg cccaaattat
     9181 acagtgcaga cgcaaaagat ccccaacgtc gccttgttta caaaagcatc gttgaagatc
     9241 ttaaaaatgg tgttgcgatt gctaagattg ctaaagacta caatgttacg agacaaaccg
     9301 tgtatagaat aaaaaaagac agcatggtca atgacaaata gttgatcatg ctgcttttat
     9361 tatcttctca tctttaattt cctacatata attactatct ataaaattaa gtcttactca
     9421 gaacagataa agcataaaat tccaacaaac cattttttac ttaactaaat aaagagtctg
     9481 ttaatataat cttcacttat ttacgttaat cccctactgt atttgttggt tccttatcta
     9541 tattttctac aaaatcctta ttatcttgtg atttagcttc gtataattct ttgtatgtcg
     9601 acacattggt tatactaatt cctatagcta gttcttgaga ctctactttt ctctctagct
     9661 cctttgacaa agtgatttga gatttatttg gttccgttgg agtcaattct atagattctt
     9721 cataaaaatc tctatacata aagctatcat taattttata aaaaaaatta aatgttttta
     9781 gcactgccat ataacattca aaacattctt ctatagttgg tttacttcct tgaggagaat
     9841 gaaccaattt attacgtttt tctatgaaat ttttaatttc ctttcttatt tctttcggaa
     9901 actcaatatt aaagtaccct ttaaacatgc tccctaataa tttgtctaca gggggagatt
     9961 gtaaattctc aaatattaga gttaaatcag gtttctcttg ttgataaaat tcctttaccc
    10021 ctatttcaca ggctgtaatt atcgaaataa acctatctct agtatttgat atatttttac
    10081 ttcttttaag gagtataaga gaaaagggga tactttcgtg tccatgcatg taattaacta
    10141 attgttgagc ttcagaagtt atatctctaa ttatatagtt aacggaatta ggaagtgtct
    10201 ttaactctaa tgaaatttct atccaattac tatcttctaa tctgtatatg ctcgaagtat
    10261 aatttgattc gtcagcaagc atcgctacat cccaaacact gatcataaaa tcttttaaac
    10321 gttcaatcat ttcagcaata ttgctaaaat cagctaattt ttctttagaa taaaaaaaac
    10381 atttatatca gaatcaataa attctatttt tttattacta tatcgtcatc attatcttga
    10441 ccatttatgt ttattgttcc ctgagtgggg agtacttctt tcaaattcaa gttaaattta
    10501 tattccatta cttctcctcc aatatataaa aattacttaa ctgtatccaa atttaatatg
    10561 taaataaatc attaattttt agctaaattc tatgagaagt ttatttttgt tgtttttgct
    10621 aattaactaa aactagtaca aaaaatttaa aatagattat taaacctatt gcttattcta
    10681 ttcagtcata tatataagcg tcatccttta gtaactaaca tcccacacta caatagtatt
    10741 ataacatatc tattcaactt caaattgttg aaatttaagc catttatatt tacttttcct
    10801 caactttaga aatgatttta ctaaccttat atccgtaata cgcgtaacaa acacattcat
    10861 taataacaat caacactaag ctatattagc tttttaaatg actagggtaa ataaaataag
    10921 gatcaaaaac ttttaagttt ctgacccttt ccttacttcc gattggttaa tagtgatctt
    10981 ttgcagaata aataaatatc gtttcatttt caactctata tatcagtcta tgttcatctg
    11041 taattcttct ggaccatttt ccagataaat catgctttaa tggctcaggt tttcctaatc
    11101 cagcaaaggg ggaacgatcg atatctttta ttaacttgtt aatctttttt atattgcttt
    11161 tgtttccttg ctcatgccaa taaagataat catcccaagc atcatcagac caagccttaa
    11221 tcatcagatt caacctcgat taagtcatgt gttttaaatg cacctttgga gaattgttca
    11281 tctcctcgac gaattttttc catgacgtaa ttattagaaa gtgttctcaa cgtttcttgc
    11341 atagaatcat aatctctttt tgataataca acaactgtat cttctacatc tttacttgtt
    11401 acaataagtg tttcagcatc ctcattaact tgtttcatat aactacgtaa attttggcgg
    11461 aaatttgaat aagctactgc ttccattcct ttcacccctt atttatttct ataaaacaat
    11521 tgtacattat attgtacaat taagcaaatg attaatattc taacttctta taattaaaag
    11581 tgctatcact tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc
    11641 aaatcctgct ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc
    11701 acttttgccg tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt
    11761 aacgggtaac cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa
    11821 taaccctatg aaaaatatgg ctgcaatcat cttgaatagt agactcacaa gggctaatac
    11881 aaagcgccaa aagatcaaac aaaataccag tattgccaaa aatactacga agtttaaatg
    11941 cacagctgca gcatggcgca taagtatttc gccaacaaat aggactaaca atagaggaat
    12001 gattttattc atatggatct ccttctttct cttaggttat cgggtgttgc ccgcttattt
    12061 cgcaccacct aagcggcggg ggcggttgga gcgattggtg cgaaataaga accattccct
    12121 tagttactag gttttttgtc cttatttcac acattctcac ttttcggtat aaatgattat
    12181 tgcctttttt tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg
    12241 atttcttctg tatcgtcgta agtataggtg tctaaaaaat cagttaaagt cgtgtaccag
    12301 tcgtcgcctg ggttatctac ttcttctaca tattccctca aaaatgcttc gggtacagcg
    12361 agttcataat ccattcccat taacacgata gattccgttc cacacgtcaa aaatctagta
    12421 aatacaaggt aataatcgtc tatcgtcgca tctttgctgt ttagctcttc catttcttct
    12481 tccattaatt ccatgctctc aaaaattgaa taatattctg attctgagta atagtgtttc
    12541 ctgcaaatcg tgtagctgac aaagttttcg accagctcac tcctttctag gtaaacattc
    12601 atgatgtatt tttctgtttc tagtcgtaga agctccgttc ctttttttac cgtttcagga
    12661 cgacgtttat acacgtctaa acgattatgg ctgactgtga ccttcgtttg actcgtttca
    12721 ctttccaaat acaattgttc ttcgggttct gttgcaaagt tttaaatcta ctatcaaata
    12781 aggtagaata atagaaaaag atagcaggag gaatgacgat gaatcatttt aaaggaaagc
    12841 aatttcagca ggatgtgatt attgtagccg tgggctacta tcttcgttat aaccttagct
    12901 atcgtgaagt tcaagaaatc ttatatgatc gtggcattaa cgtttctcat acgacgattt
    12961 atcgttgggt gcaagaatat ggcaaactac tctatcaaat ttggaaaaag aaaaataaaa
    13021 aatcctttta ttcatggaaa atggatgaaa cgtacatcaa aattaaagga aaatggcatt
    13081 atttgtatcg agccatcgat gcagatggtt taaccttgga tatttggtta cgtaaaaaac
    13141 gggacacaca agcagcctat gcttttctta agcggttagt gaagcagttt gatgaaccga
    13201 aggttgtagt cacagataaa gccccctcta ttacaagtgc ctttaagaaa ctaaaagaat
    13261 acggctttta tcaagggaca gaacatcgta ccattaaata cctgaataat ttgattgaac
    13321 aagaccatcg tccagtaaag agacgcaata aattctatcg aagtttacgc actgcctcta
    13381 ccacgattaa aggcatggaa gccattcgag gattatataa gaaaacccga aaagaaggca
    13441 ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt attgggaatc ccagcttaaa
    13501 tcatagatac cgtaagggat tttattcttt atttaaaact ttgcaacaga acccttcttc
    13561 ttcttcaaag gtatccacgt aacggggaat gtttaagttg taatcattct ccacaatttc
    13621 atcaaattct gcgacataag catacttgtc tttaattgta cgggttttat aggcctctaa
    13681 aatagcgtca atatggtaat ctcgtagcgt attttgcgtt tttatttttt caaaatcttg
    13741 tgacgcatca ataaagagca catctttttt cgcacggtct ttcttcaaga taattacggt
    13801 cgttggaatg gacgtattaa agaagatgtt agcaggtaag ccaatgacgg tgtcaatggc
    13861 accattttct aacaaggctt tgcgtatctt cccttctgcg ccccctctaa agagcacccc
    13921 atgtggcaaa acaatggcca tgacgcccgt atctttcaaa tgatagtagc catgtaataa
    13981 gaaagcaaag tccgcttttg atttaggggc taatacgcca taggcagaga agcgtgggtc
    14041 atctaagaag cctttatcag ccgaccattt tgcagaatac ggtgggttca tgagcacccc
    14101 atcgaagttc gttggctctt ctgttggcca gtcttgatcc aaggtatccg cattatgcaa
    14161 gtgttggttg gcaatgggaa ccccatgcaa aatcatgttc atacgcgcca aattataggt
    14221 agacgtattc aactcttgtc cgaaataaga aatggttccc ggttgatggg aatatttttt
    14281 agcgttcaaa agtaacgagc ccgaacccat ggtcgcgtcg taaacagaaa agcctttttt
    14341 atcttctttt ccttgtaaaa caatttgtgt catcaatttt gcgaccggtt gtggggtata
    14401 gaattcaccg gcttttttac cggattcaga tgcgaattgg ccaattaagt actcataggc
    14461 atcgcctaag acatcgccag catgccccgc catgtttaac ccttctaatt ctttcataac
    14521 atctgcgatg gttttatttt gtttttgtgg ggttgacccc agttttttag aatacagatc
    14581 aatgtcttca aataagttct caaagttttc gctggattgt tcaatgtcac ggaaaccttg
    14641 taccaggtct tctaattgaa aagatccatc atgaatggct gccacttgcg cggtaaaggt
    14701 caatgcgggt gcgatggtat atgaaaactc atcttttagt tcatacaaga gatcatcctt
    14761 tatatctgca tctttaaatg cttcaacgta aaggtcttgt gctttattta ggtcgtccgt
    14821 ttcttcctcc aatagaacgg agacataccg cagcatgttg tcggataagt atttataaaa
    14881 gaccaagcct agtaaatagc tcttgtattc attagcgtcc atttttgaac gcaggatatc
    14941 tgcggaattc cacagtgcct gatataagct ttttgaattt ttttcgattt gttctgtcat
    15001 tggttatttt cctcattcgt gttgtatttt ttataaatca gttttttaaa aaattagtta
    15061 ctatttgaac ttgttttttc accatatgta ttttaatgaa atggataagc tacatttgct
    15121 tagacaattc atgtcttact taattcttat tataacaaac ctatttcacc tttttcgata
    15181 cattaaataa taagtaactt aaaataaggt tcgaataacc ttgttaaata gtggttttct
    15241 tatcttatag tatagaaatc aagagattac ttccgatata acattttttt aaaaaaatta
    15301 gattgtaatg ttttttatat tcattctaaa ttttcactta aaaaggataa tcattaaaat
    15361 catcaaattc agtaatatat attttcttac tcattttaat atatttctct tcgtctttaa
    15421 tggtcccttc ataaaaagat ttcttagcat cttcatttag ccagtctaca gaaaacatct
    15481 gattagagac aggatctttg attttagata caaattcgga tgataaaaca ctatcaaaca
    15541 tttctccaaa aatggtggtc aatctttgaa accatatcat cataaattca gtatttggct
    15601 ttaaagcata tttgtttata atcatttcaa ctatttctct agccttactc tcattttcga
    15661 aatttaataa ctgccctata attaagacac actgcggcaa agaacccggg ttatttatca
    15721 tgatatctac caggatacca ataagtggta aaagatcgtt cggctttgaa gtgattttaa
    15781 taatgcgttc tttattaaac tcagaaagtg cttttttcaa ttggcctgaa ttaggatgct
    15841 tatctgctaa cttttttatt tccaataaat gtttttgtaa atttatacta taacgaattt
    15901 ctctatcacc atctaagttt gtggcattca ttcttatact tgccttaatt tcttcccaat
    15961 agagcttatc ttttttgata gaatctatga taatatcatc tgataatttt gttttactag
    16021 aatttaactt aaaatttaaa tcatataaga cttctgataa cactttcata atttgttcca
    16081 aatcattacg attatttgaa aatattctat aatcatctct atatctgagt actttgtaat
    16141 cttttaaatt ttctaatttt tttaattctt tcgataatct caaatcaaca taccctagaa
    16201 cgatctctgc tacaaaatcc ataagaacgg aaccctgagg gataccattt gtttgattgt
    16261 gttgcattcc agatattttt ttatctattt tatcgccaag tgagcccgaa cacttatttt
    16321 catcattttt aactttttct ttaccataca tggcccatgt tatagagtga gtataaattg
    16381 atggataaca atcagcgatg tcagtaaaca tgcaatattg gtatttaata ttctgagcta
    16441 agcttttttg ttcaaaatat tcccaccagt ttagtattgt ttcaccaaga tctgtccttt
    16501 ttgaaccttt ggaagcttca acaggtatag aataacagtt aatattttta tcttttttaa
    16561 attcactaaa cctctttttt aactcctccc aatggtcttc tttagtgaga caattaacca
    16621 aatctacata tagaatagga tgtagtagaa cgagaggacg ccatgaatag tttccatctt
    16681 tattgataat aagcgtatag tttaagtcat cacgttcttt atacattcgt gtatctacaa
    16741 tatcttttat atctttcggt tggttttttt tgaagtatta tttgtcaaat taagcgagac
    16801 aaaactttcc atttacgtaa ctcaaaaaaa cttctaatgg tgtttggtaa tttagtgatt
    16861 ttctagggat atggtttctt ttggacgcaa cggatgagat gaatccttga ttgacttggt
    16921 tgaattccat ttcttttggt agtccatctt ttcggaggag tccgtttgaa tgttcattta
    16981 atccccgttg ggaaggcgtt cctgggtctg cgaaataaat atcaatatca ttcgtattac
    17041 taatactttt ccaattagag aattcttttc cacaatcaaa ggtaattgat ttgaaaagat
    17101 ttttgggtac ggattgaagc cattcattaa tcgaattttc aatatcaact gccttacggc
    17161 cttctggttt caaggcgata atggcttttg aaagtcgctc aacgagtgtg atgacggcac
    17221 ttttatggtg gatgccgaca atcgtatcac cctctaaatg tccaaactct tcttcgaaaa
    17281 cgacataatc ttttttacgt tcagaaatat ttcttttgaa tgcctgtttt ccacgttttt
    17341 ccttataacc atttggtttt cgttttcctt gcatcggtaa atgtaaaaca ttgaattctc
    17401 ccgtcttaaa ccggcgatag agggtactta ctgaacaaga gaaggttcgc tctgcacggc
    17461 caataatgac gtctggggtc caaccttcag ttgatctttt ttcaatgtat tctttttcct
    17521 cagcaggaaa aatgatttcg gttctcccac aacgtcgctt attttctttg tattgttcaa
    17581 agtattcaag tgctgttcca ccacatttga gaaagttata gacattgtaa attgtttggc
    17641 gtccacgttt aagctgcttc gcaacgatag caaccggagt ttcttgatga aaatatgctt
    17701 ctatcattac aagctcgttt ggtgtaagat gggtataagt catttatgtt cactctcctt
    17761 gtatgcttta gcgggtatta caatttgagt gtaacataaa tggctttttt atttgtctcg
    17821 cttaattata caaacggcga agttaaaact tttttagcag attttaaagt ttcgtcaaaa
    17881 ctaatatatt ctggtagctg agcattcaaa tagctttcag acttcagaag aaaattacga
    17941 gcatttttat agtccatctc taataaagtt attctttttt tatccaatac attcagctca
    18001 attccttata atattctatt ccttagtatc tcaacattat ttactttata aaacgaataa
    18061 attttgtagt tctaattcaa atattgcatg ttatatctaa tatgggagca ttttataatt
    18121 ttcattgtga taaactcgtt attaacttta ggatacacta gttaggatta aattaggtat
    18181 ctagaaattt acttaagatt tttttgttta tttcgtatac acactcgtat caaaaacaat
    18241 cgattatgag acaaaatgaa atgacctctt ttattaaata aaagaggtca tattttgtat
    18301 cataacttgt tgcattaact atgtatctat aactcccttt tatgatacaa tcaagccaga
    18361 attaaagtac gaaaggaaga aaagcatgag tggatacatt tatggttatg cccgagtgag
    18421 tacacgttca caagaactaa atcgtcaatt agacttactc aatgaacaaa attgtaatga
    18481 aatcctaact gagaaaatga cgggaaccaa agcggttcgt ccccaactaa atcgtttaaa
    18541 agataaaatt cgtccgggag atacggtaat tgtagaaagt ttttctcgat tgggtcgtag
    18601 tacaaaggat ttgattgacc ttgttaccta ttttgaagaa caccatgtta agctggtgag
    18661 cttgaaagaa aacttcgata ccagcacgcc tcacggacgc ttaatgatga ctgtcttcca
    18721 agcatttagt cagttcgaac gtgatttaat tgttgaacgt accaaagaag gtttgaaaag
    18781 tgcacgtgcg agaggtcgta aaggtggccg tccacgagtg aaccagaaag atgttgatcg
    18841 agcagtaaaa ttgtataaaa gtcaggttta tagcgtaaaa gaaattacgg aaatgactgg
    18901 tattagcaag gcaacactct accgctactt aaaagacaat agggaataat ctattttaat
    18961 ctttaggaat ctataatatt tatgggagaa ttagagtgat ttttttatta ttactaaaaa
    19021 acaatataaa ataacgcatt ttaagccctc tttgtgcata aggatgaagc ttttaatttg
    19081 aaagaggctt aaaatgcgtt ttaatttttt ctatttttca taaatatata ggttagtggt
    19141 ttaaaattat agggtattac aactctcagc tgttaaaaat tatcttttaa agtgtcgtct
    19201 atcctctcaa gttgaaacat tgggttctta cctaatcttt tttaattgtt cataaaaatt
    19261 ttcatgcgtt ccacatagta agataggtat cactgcatta tctttaattt catatgcaac
    19321 gcgatatgtc gtacctgcat acttgaatcc aatagcccaa attcctgata aatcacccgt
    19381 tttttgttct cccgaatgag gacgaactgc tatctcatca taaattatcg ttaaaaattg
    19441 ttcttttaat gttttctctt ttaatttttt tagatacttt attacgtgtt tagaaggtag
    19501 aatctcataa gccaatttct gctgccaact cctcttttgt catcggttta gaatttgcaa
    19561 cagtatcttc agcaatagat ttaaatgcag aagttaacgc attttttcta tttctaaatt
    19621 cttttaagat ctcttgtttg ctataacctt ctgaaagaat atcagccaaa atatcttcac
    19681 taaaatcaaa aaattctttt ttaggctcta caaactcata aaggatacca ttctctacaa
    19741 gcttagcttc tacttcaaca ccattgggca catcgaattc tttagggacc gttaacataa
    19801 tagaatttcc ttgttttctt gtttttgtaa tcatattatt tcctccatta ttttacattt
    19861 cgtaattact tagtaattac attgtacgct atttttagta gaaatacaaa ataataaaac
    19921 tcctttagaa caattttcaa aggagtttta tttgattagg aaattatgta gaataataat
    19981 atgtttttaa cttggaatca aaaacgcact tgcaagtaag tttacttcaa tacatgaaat
    20041 tgcttataat agtgtattgg aattaaggta gattgacaaa agagtgatca aataggtata
    20101 cttcattcaa ataatcgttt gaatgtgagg tgagatgaat gaaaaaagtt tgccaagtga
    20161 ctgtcgtaca taaagaaaaa gtaaatggaa taaaaaataa attggatcaa aaagattttt
    20221 caaacttact tgtgttaggg aaatgtttca gcgatttttc tagaattaaa attttctatg
    20281 ctttagaaac ctataaggaa atgtgcgtct gtgatttagc agaaatactt actgctagtg
    20341 tcgctacaac gtcacaccat ttacgttttt taaaaaaaca tggaatggca aaatcacgtc
    20401 aagatggaaa agtagtttat tactctttag cgaatgaaga catcctttcc gctattcgtg
    20461 ttttttaaag ttatcagaaa atctatctat gaaatcttag tttgggggta ccgtcaggaa
    20521 aatgcggatt tacaacgtta aggaaattta ttaaagagac caattcctca gcactaaaat
    20581 tgataattgg gctttctttg ttactatagt gttaggtaag ctttttactt tatcaccggt
    20641 tttcccctca cttcacacct acgggcgagt ttccccacat cccgttttcc atctaatcca
    20701 attcattcaa tatctttatg tgtatgaaag atcagattgc ttgatttaga catcttacac
    20761 gtaatttatt cttaaatggg tacggtaaac gagcaataat acgagcaaaa gtatacctac
    20821 aaaaataata actctgtcta attttttttg attcatatgc ttattgctta aaccatataa
    20881 tttcagtcca agaaagcctc caacagtatt tgtaattaca tctgttatgt ctgtcgctcc
    20941 aatagcgaag ataaattgaa ttatttcaaa agtaagactt aaaaccagta caaaagcaaa
    21001 cttaggtaaa aatccgattt ctttaaaatt gacattcaaa agcaagccaa atggaataaa
    21061 gattataaca ttatctatca tctctctgaa attcccagta gcagtaaatg gagtcaagtt
    21121 aagacttctt tgatgataat taaatactga taaaatattg tattgtaatt tgaataacac
    21181 taaccagatt agtgtcacta aatataaagc tagcaatcct ctagataata tttttcccaa
    21241 tataatttcc tctaattctt ttgtttaagt tgtatcatta gtatagcaat gtcctagtta
    21301 acgtgggttt taaattaaaa agctaacgtt accccaaaag tgctagtaca ataatgctgt
    21361 tcattaattt cgttggtttc attccgccat ccttacctcc ttgaattagt atgtgttgat
    21421 ccgggaaaca ctgtcacaat tacaccgtct atattgtttc ctgatatctc ataacgaaga
    21481 ttagtcggca catgaatggt ggtattttta gtaacaggat aataaaagat ctcatatttt
    21541 tccccattta cactaacaga aatggttttt tcttctttta ggtaatccat atattcctcg
    21601 agaacgaaat tcttttcttt cataatcgca ctatgtggta aaccaacata gcgaatatgc
    21661 catagttcat attgaattcc tgttaactct gttttgtcct ctggataacg taaaatgaac
    21721 ccgtatttcc aagcattttc ttctatccac tttccttcag gggctcgttc cattttcgtc
    21781 aagcttgatc ctacatctag tgataaacct gaattatgct cactataacc tgctggtaag
    21841 gcatactcag cccccatttc ttggtaaagc acactttgct catcaaagtc tcgatagcca
    21901 ctattaataa taaaatgact aacgccaccc tttacagcat cattgaccat ctctgaaaat
    21961 ttttgtgcta tttcttttga catataaata ttactatcaa gcaacccgta tccatttatt
    22021 aattcgtcat gtttagataa attcacgata tctgacttca cactttcttg gcgaacagga
    22081 tatttactat tgattaatag cagatttcct tgataaacct gttcttctgt aattgttttc
    22141 tcttgggtat tttcagaagt cccactattt tctaaatgtt ctttgggatt ttgatcataa
    22201 ttttgaaatt cgactttttc ctgagaaaac agtgcttcat taacgtagtc ataacctaag
    22261 tatattaaga ataacaataa caataaaaaa aacaacttct tcattttcag tctcctcctt
    22321 aactatatag ttggaagtat gaaatatttc tagaatcttc cccttaaacg gcacaatctc
    22381 gtttgagatt gtgccgtttt gccccgtaaa ttacagtcaa ttatttcgcc ctactggatt
    22441 tactattatc accaatgtag atgaattttc ataagtgata cccatgctga atttaacgag
    22501 gctgaactgc ctgttgacaa tgggcggtgt gaaataccac ccctctatgc gatcagtctt
    22561 attatcaggc tatcttgccg cagcgcacgc agataatgac cgcaatatcg ctctgcctta
    22621 catcttcaat actatctact atatataaat tccctaagaa ccagttacat acgtcgggtt
    22681 tcctgccgaa agagttagct tatatagttt gtccgtgcaa cggttaaaag tttatttaac
    22741 ggggaaatca aaatagctat tggggtatgg ttcgtctctt aatacatagt gccaccattc
    22801 gaggctatat gcttcaaacc cactgttttc catgatggag cgcaaacgtc tgcgattttg
    22861 cgcttcattg catgatattc catttgccgc atgatgagag cgttcatcca taaaatcaaa
    22921 tcggctcccc attggtacaa gctcacccgt gtctaatcga taaagcgtaa gatcaatggc
    22981 actgccgcgg ctatggcttg attttgaagc cacgtatcct tttgaaatca tctcagttcg
    23041 gtcaatattg ggataataac tttcctttgt caggttattt tccggctgtg cagcccattg
    23101 cataaaacag tttacagcac gattaggacg gtaaccgtcc catagaagca atccgtaccc
    23161 ttgggtagca gccagttctt ttgccttcaa aagcgattca gccaactcgt atgtccctac
    23221 aatgcgattt acttcataac cgtcaaccgg ttttccggtg aaattatccc aagtggcata
    23281 tttagcgtcc caacgaacac cgtgtactat ttcatctaaa aaagtaaatc ctatttccat
    23341 gcttatcacc cctttaacgc taatacgatc aagcggtcaa tcagttcggg aagtgcaata
    23401 cctgcagcgg ccatcatacg gggataacga ctgtatgacg tgaaaccggg cagagtattg
    23461 acttcgttca gtacaatgcg gccgttatct tgtaaaaaca tatccacacg ggctagacct
    23521 ctacagccga gcgctttata tatttttttt gccgtttcct gtatccgtcc tcgctcctct
    23581 gctgaaaggt ctgcgggaac ggttataact gcgttttcag agcctttttc cggctcgact
    23641 tcctgatgaa tacgaaagat tccgtactgc agcctgattt ggtccacctc gccaacagct
    23701 aacgcggcac tgtttcccaa taccgcacaa ccgacctcac agcccgaaac agcctgctca
    23761 attaagattt tgctgtcata ttgtcttgcc gattcaattg cgtagtccaa ttcgtccgcg
    23821 ctattgactt ttttcacacc gaaggatgag cctgaacgcg ccggcttaac aaaaacagga
    23881 taggtaaacg tagctgccac cggcctatca tctttattaa taacccaaaa ggcgggagta
    23941 gctatcccag catttttcgc aacgatgtat gtcaacgatt tgtccataca aattgctgag
    24001 ctttgaatat cgcagcctac aaaagggata ccggacaatt caaacagacc ttgtatggat
    24061 ccatcttcac ctgacttgcc atgcaaagct gaaaatgcta catcaacatg gttgatttca
    24121 tattcatggt tctttttaac aagtaatccg tgcatttttt tatccggcga gagtacagct
    24181 gaatagcaat tgtcgttttc ccattccgcg caaggttttt cgcacatttt ccatacacca
    24241 gatttcgtaa ttccaatgta taacggctcg tatttttctt tattaatgtt agcggctatc
    24301 tctattgcag attttaccga tacgtcatgc tcctctgagc aacccccaaa cagtattgca
    24361 acttttattc tattcatgct cctgtctcct ttcaaaatcc aaacagtttt taatggtttt
    24421 ttcaacggta tcacgcaacg cttgctcggt ataataggcc gtatgcggtg tgattatcac
    24481 gttaggcatt ctttgaagtt taagtaaaaa ttgattatca attggttttt gggtgcaatc
    24541 agagtagaaa aactcttcct ctccttccaa tacatccaat gcggcaccgc ccagtttccc
    24601 gttttctaat gctttaacca actcataggt atctacaagt ggaccgcgcc cagtattgat
    24661 aagaaatgct ccttgcttca ttctctgtat ttgttcgtgg ctgataatat agtgcgtatc
    24721 cgtattgagc ggcacatgaa gcgtaacgat atcgctattt tgcagcaact catcaaacgg
    24781 tacatagttt acctctatac ttcggctgcg actataagcc aacactttac atccaaatcc
    24841 tcgcagccgc tcaataaccg ctttgcctat ctggcccgtt cccaccacac caactgtcat
    24901 gtcgctgagt accttgccac ggtcgctgtc caacctgaaa tcatgttttt ccacagagcg
    24961 cacaatcgat tttacgttgc gtactgccat aagaattagc atcatagtat aatcggcaac
    25021 gctatccggc gagtacgcca cattgtcgac agtgatgccc attctcttag cagcagttgt
    25081 atctatatga ttgcagccga tgcttcgggt agaaatatat ttcacaccgg ctctcttcag
    25141 cgcaagaaga atagaggcgg aaatctctga tttatgtccc acactgatac attgattgaa
    25201 aggcgcggat ttggcgttgg attccgacac gttggcgtta attatcgttg ccataacgcc
    25261 aaagcgaggc gaaagagcat ggaatgcatc tgcctcatcc tgctcacatc cataaacagt
    25321 aatgccgatg ttattcataa ttaagaccaa ccctttctgt gaaaggcacc aaataaaacg
    25381 gggatgccaa tggtaatctt gtcaaatctt tctctgaaga acgaaaacgg ctcgttctta
    25441 cggtattaag tctaccgtgt aagaacgagc cgtttcttaa tattaaattg ttaatttcct
    25501 aagaaaaagt aaagataacc ttgagatttt cctaaaaaat tatggctcta tactttttga
    25561 tgttggtgga tgacaccagc atctttttta ttttccaaaa catatagaaa agacaccttg
    25621 aaatccttta gaattaattt ggcgaaccaa cccaaaagga ggaatcaaga tgtcttacac
    25681 tcaccttatc aaagaaacat tagatattct agacttatca gttactttta acgaaaattg
    25741 tctcacaaaa gaaaaataca aagggcaaat ctgtcatatt tatcgtggaa acttgattta
    25801 tacagcccaa gaatgcatcc actgtaaaca ccagattgct tcagatattg tgcgttgggg
    25861 tacaacaacc gtccggttgt taatgaacga tgtttctgaa taccgtacct acctcgaatt
    25921 gaaaaagcaa cgcttcaaat gcaaagcttg tcagcggacc tttgtcgcag atacttctgt
    25981 tgcgaaaaaa cattgtttca tcagtgagaa agtccgctgg tctgtcgtga ctcgtctgaa
    26041 aaaaatacct caatgacaga gatcgccgct cagaaaaatc tatcggtctc ttctgtttac
    26101 cgtattatga agcgatttta tcgtccgttg aatccttttc ggacaccgtt acctaaggta
    26161 ctttgttttg atgaattcaa atcagttcgg ggtgtttcag gtgctatgag tttcatcatg
    26221 atggatggac aaacacagcg cttattggac attgtcgaga accgacaact gcctttttta
    26281 aaacgttatt ttagtcattt ttcacgagaa attcgtgaag cggtcgaatg gatcgtgatt
    26341 gatatgtacg ctccttatgt atcgctcgtt aaaaagctgt ttccaaaagc acagctgatt
    26401 attgaccggt ttcacatcgt ccaacatatt ggtcgtacct ttcgcaatca tcgaatcaaa
    26461 gaaacgaacc aactattaaa aagcaaagaa caaaaacact atcaattagg taagcaatta
    26521 aaaagatact ggaaacttct tcaaaaagat gaacgcaagc ttgattatac tcgacgcctt
    26581 tggcgacctg gcttcaaagc tcatttaaca gagaccgata ttgtggatcg cttactgaaa
    26641 gggagccctg ctttgcgtgt aggctatcag ttatatcaag actttcttta cgctgtaaag
    26701 gaacgagact acgtatcgtt tgaagaacta cttaccaata atatcatgct tcccgaaggc
    26761 tatcagacga ccttgagaac gttccagaaa tttctgccac aaatcaaaaa tgctttacaa
    26821 caaagttatt caaatggccc acttgaatgt ctgaacaacc atatcaaagt gttaaaacga
    26881 aatgcatatg gctttcgtag cttttataat ttcaaattgc gcatcatgat tcgtcatggc
    26941 aacgcattga ttttcaacta aaaaagatca agaggaactg atccacttga tctccaatta
    27001 ttctaagatt tctctttacc aacatcattt gacaaagagc caaaattata tacatctctt
    27061 aggacctcct tttatcaacc aagtctggca tcgctggaag ctctacccta aacgtcgtat
    27121 agttatcatt gctttccgcg taaatctgcc ctccatgctg aacaataatt tcttttgcaa
    27181 tcgccaatcc aagtcccgcg ccacccgtat cggaagaacg ag
//
