LOCUS       CP063548               10412 bp    DNA     circular BCT 04-APR-2022
DEFINITION  Enterococcus faecium strain BMT-4-1-1 plasmid pRL-1-1_06, complete
            sequence.
ACCESSION   CP063548
VERSION     CP063548.1
DBLINK      BioProject: PRJNA669626
            BioSample: SAMN16483166
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10412)
  AUTHORS   Both,A., Kruse,F., Mirwald,N., Franke,G., Christner,M., Huang,J.,
            Hansen,J.L., Kroger,N., Berneking,L., Lellek,H., Aepfelbacher,M. and
            Rohde,H.
  TITLE     Population dynamics in colonizing vancomycin-resistant Enterococcus
            faecium isolated from immunosuppressed patients
  JOURNAL   J Glob Antimicrob Resist 28, 267-273 (2022)
   PUBMED   35134550
REFERENCE   2  (bases 1 to 10412)
  AUTHORS   Both,A., Kruse,F., Christner,M., Huang,J., Mirwald,N., Franke,G. and
            Rohde,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) Institute of Medical Microbiology, Virology
            and Hygiene, University Medical Center Hamburg-Eppendorf,
            Martinistr. 52, Hamburg, Hamburg 20246, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Trycycler v. v0.3.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 95.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/27/2020 16:17:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.13
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 3,154
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 78 of 160
            Pseudo Genes (incomplete)         :: 71 of 160
            Pseudo Genes (internal stop)      :: 30 of 160
            Pseudo Genes (multiple problems)  :: 18 of 160
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..10412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="BMT-4-1-1"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pRL-1-1_06"
                     /geo_loc_name="Germany: Hamburg"
                     /lat_lon="53.33 N 10.1 E"
                     /collection_date="2015-01-07"
                     /collected_by="Institute of Medical Microbiology, Virology
                     and Hygiene, University Medical Center Hamburg-Eppendorf"
     gene            1..933
                     /locus_tag="IQN18_15715"
     CDS             1..933
                     /locus_tag="IQN18_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UOC27613.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIRF
                     YFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIHGE
                     VDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWAEFS
                     KEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFTW
                     KAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEADFKKQQ
                     QTEKEEAEKQSISNELKQDLLENLQNLFD"
                     /name="replication initiation protein"
     gene            950..1420
                     /locus_tag="IQN18_15720"
     CDS             950..1420
                     /locus_tag="IQN18_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="UOC27614.1"
                     /translation="MTDKTIKELAEEFHVSKQAIRKRLSADFRENYVQTVTRNGVKTLV
                     VSYKGYLLLKQHFSGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLDQQ
                     QQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKENNNAKTTEKKWWKFW"
                     /name="DUF536 domain-containing protein"
     gene            1504..1808
                     /locus_tag="IQN18_15725"
                     /pseudo=""
     CDS             1504..1808
                     /locus_tag="IQN18_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /name="helix-turn-helix transcriptional regulator"
     gene            2002..3180
                     /locus_tag="IQN18_15730"
     CDS             2002..3180
                     /locus_tag="IQN18_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
                     /protein_id="UOC27615.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDYE
                     KYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEETV
                     IHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVRTLNDKYVAIFMD
                     ATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDIYSRGVHDV
                     LLFITDGLKGMKDTIHQIYPKAKYQHCCVHVSRNIAHKVRVKDRKEICDDFKAVYQASS
                     KEEANTFLGSMIEKWQKTYPKVTQSLIKNQDLLTFYEFPPGIRRSIYSTNLIESFNKQI
                     KKYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE"
                     /name="IS256-like element ISEfm2 family transposase"
     gene            3415..4596
                     /locus_tag="IQN18_15735"
     CDS             3415..4596
                     /locus_tag="IQN18_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="UOC27617.1"
                     /translation="MKNNRIYKNQLNYGLLLPVFLLCIIGLLSLYVALYHDSYTNSKIT
                     KELIKQVLWYSIGFTSAITIYFFNPKILWKLTPIFYGLCIIMLLLLLKFYNPQLAVLTG
                     AKNWFYIAGFSFQPTELVKIGYTLMLAKITVNYQQKSITSNTKDDWIYLLKTFIATLPI
                     GILLFFQNDLGTMLVFIVIYIFILFTSNINIKIIAPIIAIGVVLSLIFLYLVISDTGRD
                     FLLKIGFHDYQFKRIDSWLNPFLDPNGSSYQLANSLIAIGSGGLLGTGFNVSNIHVPVR
                     ESDMIFSVIGENFGFIGSCLVIFLYFYLIYQMYKTCFKSNNLFFTYIGIGIVAMIFFHV
                     FENIGASIGLLPLTGIPLPYISQGGSAILSNFLGLGFMLCCSRYTNEKKGTQK"
                     /name="FtsW/RodA/SpoVE family cell cycle protein"
     gene            4686..4955
                     /locus_tag="IQN18_15740"
     CDS             4686..4955
                     /locus_tag="IQN18_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="UOC27616.1"
                     /translation="MKKTKLLVASLCLFSSLLAFTPSVSFSQNGGVVEAAAQRGYIYKK
                     YPKGAKVPNKVKMLVNIRGKQTMRTCYLMSWTASSRTAKYYYYI"
                     /name="bacteriocin"
     gene            5761..5934
                     /locus_tag="IQN18_15745"
     CDS             5761..5934
                     /locus_tag="IQN18_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOC27608.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNRELKLNSNYYNS
                     PNEELFVKETDK"
                     /name="hypothetical protein"
     gene            6384..6608
                     /locus_tag="IQN18_15750"
     CDS             6384..6608
                     /locus_tag="IQN18_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="UOC27609.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEKC
                     WVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
                     /name="bacteriocin hiracin-JM79"
     gene            6628..6915
                     /locus_tag="IQN18_15755"
     CDS             6628..6915
                     /locus_tag="IQN18_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="UOC27610.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKSV
                     GEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
                     /name="bacteriocin immunity protein"
     gene            7355..7735
                     /locus_tag="IQN18_15760"
     CDS             7355..7735
                     /locus_tag="IQN18_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UOC27618.1"
                     /translation="MSEQNQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            7717..8631
                     /locus_tag="IQN18_15765"
     CDS             7717..8631
                     /locus_tag="IQN18_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="UOC27611.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRT
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGKEYAEKIAPEYQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLDKCYDINDEICLTHGLSITERGKKEEKRTMSEIKLKEK
                     NKPVWKDEIRFAIDQTMKNKKTRTYDQFCDSLKTFGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            8871..9560
                     /locus_tag="IQN18_15770"
     CDS             8871..9560
                     /locus_tag="IQN18_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOC27612.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFLLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARNKKKKEQALAQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYEQTKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYENLFQQATNIQSPTKKETALNYLESVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     BGC             1384..10412
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2..933
                     /name="rep_cluster_1249"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.786"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7718..8628
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.671"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggcaaatg aaatcgtaaa gtatgaacca gaattaaata ccattccttt aagaaaattt
       61 accccaatag aaatgaactt gtttttttct attgtctcta gaatgcgcga taaaggtaat
      121 caaaaaatac gcttttattt cagccaactt aaagatttaa gtgcgtataa gcagactgca
      181 ggagaagtct ttataaaaga tttggcaaga acatacgatc atttgatgga tttacgtttc
      241 ggaagaaaaa gcaatggtgg attaaccata gaacgattta caatgtttac tgattttgaa
      301 attcatggag aagtcgataa cccttatgta gatataaaag tccacgaaaa agctttgcct
      361 ttacttaata atctagaaag ctgggtacgt ttctctttaa cagaatttcg agatttaaat
      421 agcagttatg ctaaaacaat gtttagattg ctaaaacaat accgaactca aggatgggct
      481 gaattttcta aggaagattt tttagagcta ttagatattc ctaaaagcta tagacaaaca
      541 aatataaatc agtttgtact caaaccaata aaagaagaac taactccact atttaaagga
      601 ttaactattc gtaaaaaata tggtaaaggt cgaggtaaac ctgttattgg ttatcgtttt
      661 acttggaaag ctgaaatcaa ccatgctgac gatttttcaa aagggaagca agaagattta
      721 cgtataaaat tatttaatat tgaacacaat ggagaattaa cacaaaaaga aaaatggcgt
      781 gctaaagatc gaatactaaa tcttccgtta ggtactcatg aagctgattt taaaaaacaa
      841 caacaaaccg aaaaagaaga agcggaaaaa caatcaatct caaacgagct taaacaagat
      901 ttattagaaa acttacaaaa cttattcgat tgaagcataa ggagtattta tgacagataa
      961 aacgatcaaa gaactcgcgg aagaatttca tgtttcaaaa caagcaatta ggaaaaggct
     1021 atcagctgat tttagagaaa actatgtgca aactgttact aggaacggag tgaaaacctt
     1081 agttgtcagc tacaaaggat atttgctttt aaaacaacat ttttcaggta gtaacaccac
     1141 tggtaaccaa gtagaaaccg ttgctagtaa cgatgaaaac tcgattattg tttttttaca
     1201 tgaacagctc caagaaaaag atactcaaat aaaacaaatg caaaagttac tcgaccagca
     1261 acaacaactc accttgcagg caaatcaaca gatcgaaaaa ctgcaagaac agttacaatt
     1321 agcttatacg gaagaacctg aagaagagga attaacttct gtcaataaag aaaacaacaa
     1381 tgcaaaaaca acagaaaaaa agtggtggaa attttggtaa caattaccaa ctttccataa
     1441 agaagaaatc aatttctata gactttatct ttctaagaat aaaaaatatg tttaggtgat
     1501 caaatgacta taagtaaagt aatcaaacaa aaaagaatag aaaaacaact aactcaagaa
     1561 gatatagctg aaatgctttt agtatctaaa aaaaccattt ctaattggga aaatggtcga
     1621 acaataccag atacagaaaa tttaattcag tttgctaagt tatttgattt atcactagac
     1681 aatattttgt taggagaaga aaaaattatg gtaaaaaatc atacaaaaac actaatacta
     1741 ttatttcatt ttgtaatttt tttaagttta gttttcacct taattcagtc cggattcttt
     1801 tattttagcc attcaattgc cacttggtta ttaatatttt ttctaagttt gctattagta
     1861 atcaatactc tgattattat ttttaataaa cataaaaatt tatttcttca tagtgtaaaa
     1921 tattttgtgt aaatagaaaa aggaagtccc ttctgtagaa tagagttacc acaacacatt
     1981 cacagaaaag aggacttcca tatgaacgat tttactacag aaattctaaa gactctagcg
     2041 aacaaaggcg atttgaatga attattccgt gtccatttgg aaaaagctgt caatacgctt
     2101 ctcaaaacgg agttaacggc tttcctcgat tacgaaaagt acgatcgcat tggttttaac
     2161 acgggtaatt ctcgtaacgg ctcctatgac cgtacggtca agaccgagta cggggaactt
     2221 catctccaga ttccgcgcga ccgcaacggc gagttcaagc aacagactgt tcctgcttat
     2281 agacggacga atgatacctt agaggagacc gtcattcacc tcttccgaaa aggtattacc
     2341 atgtcggaaa tcgcagactt gattgagaaa atgtatgggc atcactacac gccccaaacc
     2401 atgtccaaca tgactaaagt gctgactgaa gaagtaaatg catttaaagt tagaactcta
     2461 aatgataagt atgtcgctat tttcatggat gctacttata ttcctttaaa acggcaaacc
     2521 gtctccaaag aagcaatata tattgccatt ggtatacgag aagacggcac taaagaagta
     2581 ctaagttatg cgattgctcc gactgaatcg acctacgttt ggaatgagtt gttacaggac
     2641 atttactcca gaggggttca tgatgtctta ctgtttatca ccgatggctt aaaaggcatg
     2701 aaagatacga ttcaccagat ttatcctaaa gctaaatacc aacattgctg tgttcacgtt
     2761 tcccgtaata ttgctcataa agtacgtgtc aaagatcgaa aagaaatctg tgatgatttt
     2821 aaggctgttt atcaagctag ctcaaaggaa gaggcaaata cctttttagg gagtatgatt
     2881 gagaagtggc agaaaactta tcctaaagtg acgcagtcac tgataaaaaa tcaagattta
     2941 ttgacttttt atgagtttcc gcctggtatc cgccgaagca tttactcaac taatttaatc
     3001 gaatctttca ataaacaaat caagaaatac agccgcagaa aagagcagtt tcaaaatgaa
     3061 gaatcactag agcgtttcct agtatccatc tttgatacat acaatcaaaa attcttaaat
     3121 agaagccata aaggcttcca acaagtgacg gatacattag cttcaatgtt tactgagtaa
     3181 cccattattt tgcagaagga cgagttattt acacaaaatt attgacgctc cctatttctt
     3241 cacagcatat ttccaattgt tttattaatt tttctatact ttatataaaa agatagaact
     3301 atttagtaag ataaaaaagt agctaattgt aattttcgtc taaaacttaa aactagtaaa
     3361 aataaattta ttatgttatt tttatttata aattatgaaa aaggactttt cgaaatgaaa
     3421 aataatagaa tctacaaaaa tcagttgaat tatgggcttt tattgccagt ttttctgcta
     3481 tgtatcattg gattactttc tttatatgtt gctttatatc acgactctta cactaattca
     3541 aaaatcacaa aagaattaat taaacaagta ttgtggtata gtattgggtt cacttcagca
     3601 ataactattt atttttttaa tcctaaaatt ttgtggaaat taactcctat attttacgga
     3661 ttatgcatca taatgttatt acttctcttg aaattctata atccacaatt agcagtctta
     3721 actggagcaa aaaattggtt ttatattgct ggtttttcgt ttcaacctac tgagttagtc
     3781 aaaataggat acacccttat gctagctaaa ataaccgtca attatcagca aaaatctatt
     3841 actagtaata cgaaagatga ttggatttat ttacttaaaa cttttattgc tactcttcca
     3901 ataggaatat tattattttt tcagaacgat ctaggaacaa tgttagtctt cattgttatt
     3961 tatattttta ttttgtttac gtctaacata aatataaaaa taattgcccc aataatcgcc
     4021 attggtgttg ttttatcctt aatattttta tatttggtta tatcagatac aggaagagat
     4081 ttcttattaa aaataggatt tcacgactat cagtttaaaa gaatagactc atggttgaat
     4141 ccatttttag atccaaatgg atctagctat cagttagcaa atagtttaat tgctattggc
     4201 tctggcggac ttttaggtac tggttttaat gtatcaaaca ttcacgttcc tgttagagaa
     4261 tccgatatga tattttctgt aattggagaa aattttggct ttataggaag ttgtcttgta
     4321 atattcctat acttttattt aatttatcaa atgtataaaa catgttttaa aagcaataac
     4381 ttatttttca cctatatcgg tataggcatt gttgcaatga ttttttttca tgtttttgaa
     4441 aatataggtg cgagtatcgg attactacct ctaactggta ttccactccc ctatattagt
     4501 caaggtggtt ctgccattct tagtaatttt ttggggttgg gatttatgtt gtgttgtagt
     4561 agatatacta acgaaaaaaa aggaacacaa aaatgaattc aaaataacac tttatgaata
     4621 gaatgtctat ttcaataaaa attatgatac cttaaagtta atcattaaaa aaggagtgtc
     4681 ataatttgaa aaaaacaaaa ttattggtag ctagtttgtg tttgtttagc agtttattag
     4741 ctttcacccc ttctgtttca ttttctcaaa atggtggagt ggttgaagct gctgctcaaa
     4801 gaggatatat ctacaaaaaa tatccaaaag gtgctaaagt gccaaataaa gttaaaatgt
     4861 tagtaaatat tagaggtaaa caaactatgc gtacttgtta ccttatgagt tggactgcta
     4921 gtagtagaac agccaaatat tattactaca tttagtaaaa acaaaacatt ctttctatac
     4981 aataaaatag cgtatagaga ggatgttttt aatttgaaaa ggagtggtta tctttatgaa
     5041 aaatagatca tttgctatat tagtattatc cgtaatttta ttagatatat tgttaataac
     5101 attagcttta aaaactttaa atttagtaac aagttttgtc attataccag taattatagg
     5161 aatgtctatt tatttcatca aaaaaaataa acaatttttt tgaatgaaat tacgggcgaa
     5221 aataatcact caaaattatt aataaaaacg aatcaactac tttttctgaa gaaacataca
     5281 accgtaaaca acaattgttg tttacggttg taaattatca aactatatgg tgacttgttg
     5341 cttaaaatag gttgaaattt cgtttatttt ttttgagaaa ttttacccac gtactaccca
     5401 tgtacctatc cacgtattac ccatgtaccc cccctgacaa atgggcggag gggcttgcgg
     5461 cgcttggtat taaaggcatt ctccgcctcc caggtttctg accattttct ctcaacgtgg
     5521 taaaaacccc acttatgaga gaaaatggtc agaaacctaa acgatcggca aagaaaacat
     5581 accaagcgcc acaagcaaaa aaagttagag taacttccag aaaagaaaaa ttttttagaa
     5641 caacttttga aaatttgatt ttgatttata tccgttcaat ttaaacattt gataaactga
     5701 aggctagtat ttaaaatgtg tatacaagtg gtagcgttaa aaaaagtgga ggtataacct
     5761 atgttaaaaa aaactgtgtt ggtactgggt tctatttctc taggaatatt aggttttagc
     5821 cccatgctat tatccgtaat tggaaaaaac aattccaaca gagaacttaa attaaattca
     5881 aattattaca acagtcctaa cgaagaattg tttgtaaaag aaacggataa ataataaaaa
     5941 agactgctct tccgagcagc cagttaaaaa ataatgagtg tttattcttg aaaaagcaac
     6001 gcgcttatga agcgctacca aaaatttatc atattttatg aaaaataaca ccccagtaaa
     6061 aaatgactgg taaaaattca caaagaaacg gaaagaagca actgcagaaa atcaagcctt
     6121 aaaaagagct tttgacctag accaatcgaa aatcacaaag tgagcctctg tatcggttga
     6181 tacaagggct acttggcata gtatacatta tgtctactag ctgtttaaat aactgatctt
     6241 ataattttat ttaaacgatt atgaattcaa aactactttt tatgacgtta atcattgtta
     6301 tataataaaa tgttatataa taaaattact aagattattt aatataacaa gcaattttaa
     6361 aaaaatgaaa aggaaaaaca atcatgaaaa agaaagtatt aaaacattgt gttattctag
     6421 gaatattagg aacttgtcta gctggcatcg gtacaggaat aaaagttgat gcagctactt
     6481 actatggaaa tggtctttat tgtaacaaag aaaaatgttg ggtagattgg aatcaagcta
     6541 aaggagaaat tggaaaaatt attgttaatg gttgggttaa tcatggtcca tgggcaccta
     6601 gaaggtagtg gtagggaggt tattataatg gattttacta aagaagaaaa acttttaaat
     6661 gcaattagta aagtatacaa tgaagcaact atagatgact atcctgactt aaaagaaaag
     6721 ctctttcttt attctaaaga aatcagtgag ggaaaaagtg ttggtgaagt tagtatgaaa
     6781 ttaagtagtt ttcttggaag atatatttta aaacataaat ttggattacc taaatcttta
     6841 atagaattac aagaaattgt tagtaaggaa tctcaagtat atagaggatg ggcttctatt
     6901 ggtatttgga gttaattctt tggaataaaa tgaaaatggg actgaagaat caattctgat
     6961 tcatcagtcc cataaattat agagccgaaa aattatcata tttatagatt tattaatagt
     7021 aaatggcata agaatctttg aacaacgaaa aaaatgtagc tctacctaat tattgatttg
     7081 caacaatttc accgaaggtg aaacaagcga aactttacaa aaagtggcta gcatttgcta
     7141 gtcttttttg tattgtggag agtgggggag ggggattcaa gggggagctt gcgccccttt
     7201 gacaagcggt tatttgcacc aaatacgtag gtatttggct agcaaatata gagcttgcca
     7261 aacccttttg gtgagcttta ctcattgtac ctttttaggt acaatgagta ttgcgaacga
     7321 tacgtccgta ggatgtactg aaagggtggg acttatgagc gaacaaaatc aaaatttggc
     7381 tagcgatcat tctaaaaaat atacctatcg atccgagcca aaacaaatca gttttcgagt
     7441 gagcgaatcc gaattcgcaa agctgaagca gtcagctgaa gctttgcaaa tgagtgtgcc
     7501 ggctttcgtg aaagccaagg cacaaaacgc gcgtctggtg acaccgaaag tggcgccaga
     7561 cattgcccag gcaatcgcgc gcgatttagc caaagctgga gggaatatca atcaaattgc
     7621 caagtggtgc aatacacatc aacatgacgt tgctcctgga gacgcccaac gcttatccga
     7681 aaacctaaaa atcatgcaaa aggagctaca aaagatatgg caacagttaa agtaagccga
     7741 accacttcat gtagccgagc gatcaattac gcagaaccac gagcaaccgt caaaacagga
     7801 ataaactgtg acattgatta tgccaaaagt gaaatgaaac aaatacgcac gctttatgga
     7861 aaagacgatc gtgtacaagc acacttattg attcagtcct ttcgaccagg agaaattacc
     7921 gctgaaaaag ccaaccaact agggaaagaa tacgctgaaa aaatcgcacc agaataccaa
     7981 atcgcgattt acacacacac agataaagac catatccaca atcacattgt cattaattcg
     8041 gtcaatttag aaacaggaaa aaagtttcaa gcacatggtc aagcattcct agataagtgt
     8101 tacgatatca acgacgaaat ttgtttaact catggtttaa gtatcactga acgaggaaaa
     8161 aaagaagaaa aacgaaccat gtctgaaatc aaattaaaag aaaaaaataa accggtttgg
     8221 aaagacgaaa ttcggtttgc gattgatcaa accatgaaaa acaagaaaac cagaacctat
     8281 gaccaatttt gtgattccct caaaacattc ggcattcatt gcttcaatcg cgggaaaaat
     8341 ttcacttacg aattgatcca caaaaagaaa aaagtacgat ccaacagact aggaaaggac
     8401 tacgaaaagg agacgatttt acatgagttg gacagacgag aacgaaccta taaccaacag
     8461 cgaaccaacg agctacgaag agctcgagct agcattcgcg aaactgcaga agaagtacga
     8521 agagaacaac gaactcttac gcctgaacaa ccggaaatta gaccggctcg aagaaaccat
     8581 gaacaacacg ttgaaagagg aacagatcac caacaagaat tatcgctcta aaacgttaaa
     8641 agacatagaa aacgaaataa ggaaacaacg tgcttttctc gttccttata cgcaagaaag
     8701 gatacaaact acgattgatt tacaagttaa aaaactccat caagagctat tagaccagcg
     8761 aaataaggaa cagaaaaagg tgcttattca acgatcattg gattttcttg cccccttatg
     8821 ttgcttagtc ctgacactgt tcgcattttg gttagtatgg ggaggctaaa atggcatttt
     8881 atttacccta tctactcatt tttgttagta tttcaggaag tatttggctc atctataaaa
     8941 ttttccaaac aagatattca ttaaaaggct ctaaaattcg atttaagcga tttttcttgc
     9001 ttggctgtat attttccttg ataatcgtct caagcggctt attcggcgtt ttagagggca
     9061 ataagcgcgt ttctagaagt attttattag gaaatgtcac tcaaaagtat gaatcagcac
     9121 gtaataaaaa gaaaaaagag caagcattag cacaaaaaat ggaagagttt accacttgtt
     9181 atgaggaaat gaacgatatt tttgtgaatc aagaaaaacg tttgacggat aaaaacatgg
     9241 aaaaactcac tcgtctttat caaaacttgc cagaaaaaca gcaaaaagag gttcaagaca
     9301 actatgagca aaccaaaaaa gacgtgcaat atgtaaaaga tacaaagata gaagaagcct
     9361 gtagtgatct atttggagac acaaaccctt ggtttgccag tgaagaagaa aaaaaagaaa
     9421 aacaacaatc tgtcacatat gaacgctacg aaaacctgtt tcaacaagca actaacatac
     9481 aaagtccaac gaaaaaagaa acggcattaa attatctaga aagtgtcaag gaatggttag
     9541 atcagcaaca acaaaattga atttgattta aaatcatgtt aaactaaaca tgcaaatagg
     9601 taatcatgag atacacgaaa gccctagaga agtcggattc tctagggctt ttttttttgg
     9661 ctgatgtcgc cttagttgat tttacgtctt gtttcggtta ttcagccaat aactgaatag
     9721 agagtttaga agctcaacaa gaagggcgca aataaagttg gtaatcataa gatacacctc
     9781 cttccagtcg caagactgtc ggataggcga caagttatta tatcttaaaa aattaaattt
     9841 gtgaggtgtt tttaagtatt acttgtttta gggttgttag ggcttgccct gaccgcctgt
     9901 agggcgcttt aaattccaga taaaggtttt tatactaaga ctgtcttaaa acggcttaga
     9961 caagctctta gagcctcaac cctccttttt gaacaatggt tttagcaaag gagctggagg
    10021 cgactgcgca ggtataatgt gagagcgtag cgatcacacc tttttggcga ccgtagggag
    10081 cgaggttttg aattagcagt ttagaaaagt tcgcgaacct gtttaaagaa aaaagaattg
    10141 ttttttaggt gggaaatttt gtctccctct tttataatct ttttttaaac ctctttttaa
    10201 acctctttac cttcggctgt gtccttactc ccccaaggga tacaaaattt tataggcatg
    10261 aaattatctg tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata
    10321 ggcatgaaat tatctgtttt aggtattgtt tttagacata atatgtccta aaattatgtc
    10381 aataaaatca tttagactgg agtaacaaat aa
//
