LOCUS       CP063547               10610 bp    DNA     circular BCT 04-APR-2022
DEFINITION  Enterococcus faecium strain BMT-4-1-1 plasmid pRL-1-1_05, complete
            sequence.
ACCESSION   CP063547
VERSION     CP063547.1
DBLINK      BioProject: PRJNA669626
            BioSample: SAMN16483166
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10610)
  AUTHORS   Both,A., Kruse,F., Mirwald,N., Franke,G., Christner,M., Huang,J.,
            Hansen,J.L., Kroger,N., Berneking,L., Lellek,H., Aepfelbacher,M. and
            Rohde,H.
  TITLE     Population dynamics in colonizing vancomycin-resistant Enterococcus
            faecium isolated from immunosuppressed patients
  JOURNAL   J Glob Antimicrob Resist 28, 267-273 (2022)
   PUBMED   35134550
REFERENCE   2  (bases 1 to 10610)
  AUTHORS   Both,A., Kruse,F., Christner,M., Huang,J., Mirwald,N., Franke,G. and
            Rohde,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2020) Institute of Medical Microbiology, Virology
            and Hygiene, University Medical Center Hamburg-Eppendorf,
            Martinistr. 52, Hamburg, Hamburg 20246, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Trycycler v. v0.3.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 95.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/27/2020 16:17:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.13
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 3,154
            CDSs (total)                      :: 3,063
            Genes (coding)                    :: 2,903
            CDSs (with protein)               :: 2,903
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 78 of 160
            Pseudo Genes (incomplete)         :: 71 of 160
            Pseudo Genes (internal stop)      :: 30 of 160
            Pseudo Genes (multiple problems)  :: 18 of 160
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..10610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="BMT-4-1-1"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pRL-1-1_05"
                     /geo_loc_name="Germany: Hamburg"
                     /lat_lon="53.33 N 10.1 E"
                     /collection_date="2015-01-07"
                     /collected_by="Institute of Medical Microbiology, Virology
                     and Hygiene, University Medical Center Hamburg-Eppendorf"
     gene            join(8512..10610,1..28)
                     /locus_tag="IQN18_15655"
     CDS             join(8512..10610,1..28)
                     /locus_tag="IQN18_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOC27602.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQY
                     KDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKYG
                     TPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFDTI
                     KDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTLSIY
                     HLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINIHNEAENNSLKYWERTT
                     NLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENNKEFFI
                     GQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYILVPKKY
                     KEYAKMIEKNYLEDIQFRLEDSPPQAPYKTPNLKNLKIKLIFVNNNQKYFSFNTLYGKA
                     KDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQINPYIKEFNL
                     ESIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVSFTINSKKIA
                     LKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIFLINLITSIII
                     YKKNSFKYFKENIL"
                     /name="hypothetical protein"
     gene            25..644
                     /locus_tag="IQN18_15660"
                     /pseudo=""
     CDS             25..644
                     /locus_tag="IQN18_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /name="ABC transporter ATP-binding protein"
     gene            complement(802..1709)
                     /locus_tag="IQN18_15665"
     CDS             complement(join(802..1253,1253..1709))
                     /locus_tag="IQN18_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /ribosomal_slippage=""
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="UOC27603.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQL
                     KQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIKII
                     RYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHM
                     IVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALKGITIS
                     VPPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAY
                     SVLLSGAQRRFEGTLRYSLGY"
                     /name="IS982-like element ISEfm1 family transposase"
     gene            2393..2773
                     /locus_tag="IQN18_15670"
     CDS             2393..2773
                     /locus_tag="IQN18_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UOC27606.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2755..3669
                     /locus_tag="IQN18_15675"
     CDS             2755..3669
                     /locus_tag="IQN18_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="UOC27604.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKKKVRS
                     NKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTFTPEQSEI
                     RPTRRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3914..4603
                     /locus_tag="IQN18_15680"
     CDS             3914..4603
                     /locus_tag="IQN18_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOC27605.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLVG
                     VIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTACY
                     EDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQTEEA
                     CCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLESVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(4724..4810)
                     /locus_tag="IQN18_15685"
     CDS             complement(4724..4810)
                     /locus_tag="IQN18_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017548328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="UOC27607.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            5486..6436
                     /locus_tag="IQN18_15690"
     CDS             5486..6436
                     /locus_tag="IQN18_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="UOC27597.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRL
                     TFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIRGT
                     AKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVVGYQF
                     TFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQI
                     KQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
                     /name="RepB family plasmid replication initiator protein"
     gene            6457..6990
                     /locus_tag="IQN18_15695"
     CDS             6457..6990
                     /locus_tag="IQN18_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="UOC27598.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLREQL
                     ALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEQLQLTYTEEAPENDSTTLSEATETID
                     KQIDTEKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            7006..7176
                     /locus_tag="IQN18_15700"
     CDS             7006..7176
                     /locus_tag="IQN18_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UOC27599.1"
                     /translation="MTRKQLLFGSILLGILGFSPMLLSVINKGNKRGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(7490..7951)
                     /locus_tag="IQN18_15705"
     CDS             complement(7490..7951)
                     /locus_tag="IQN18_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="UOC27600.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPDI
                     ETLKKICEFYSTNINSILNEDNLKNKQNNLLVLTAIIFNIFVTILGLFELIQMNFFSFT
                     HGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTIFFPLVILIILLS"
                     /name="helix-turn-helix transcriptional regulator"
     gene            8084..8458
                     /locus_tag="IQN18_15710"
     CDS             8084..8458
                     /locus_tag="IQN18_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="UOC27601.1"
                     /translation="MKKFLCLSALMGVLLTSGGIVSATEALNLDVPEDHTAIYGGGMEA
                     TEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKAGV
                     RAYATCNATLTGNTGWWNVY"
                     /name="lactococcin 972 family bacteriocin"
     BGC             3084..10610
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          5488..6436
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.953"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2756..3666
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.408"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 taaatatttt aaggagaata ttttatgata ttatcagttg agaatttcac cctgtcaatt
       61 aataaaaaaa gatcaaacaa aattggaact tttcaatatc agaaaaagat tttattatag
      121 taacaggaaa aagtggtagt ggaaaaacta ctctgttaaa taatttaagc ctaatagata
      181 aacaatatac cggtaactta ttctattttg aaaagaaagt ttctaaacat gatatacaac
      241 gtctacgaag aaatcatatt agctatttat ttcaaaacta tgctcttctt gaaaatcaaa
      301 cagtacaata caatttcgaa ttagcaaaaa agtttaacag taatatttcc aatgatcata
      361 tttataattt attaacagat ttttctttgc caaaagatat cttaaaagaa aaaatctttc
      421 tcttaagcgg tggagaacaa caaagaatag cattaattag aaatatgtta aaaccatttg
      481 atatattatt tgcagatgaa cctacaggaa atttagataa agaaaactca acttttataa
      541 tgaactttct gaaaaattta acagaaaaag aaaaaaaagc cgttgtacta gtaacacatg
      601 acttaaatgt tctaaactat ggaactaata ttatagaatt ataattttta cccgtgatgc
      661 tagctgattt aaaactagca ccacgggtat ttattttata ttccatctct cctatcccaa
      721 aatttaaata atcacttaat aaaaaggagg cacccaagtg attttattga aaaaagagta
      781 tacccgtggt gctagttaat tttaatatcc taaagaatat ctcaaagttc cttcaaaacg
      841 tcgttgtgct ccacttaata gaacactgta agccagtaat atgctttcaa atctactctc
      901 tagcccttta acagaacgtg agttgaaatt ttgacaacct aatttttcta aggaagaaaa
      961 aacagtctca actgtttttc tttgttttcc aagtaagctg tggtctagtt tttctgattt
     1021 atccatgttt ttacgtggcg gaacggatat agtaatgccc ttaagggcaa gcttttcatg
     1081 gattttatgg ctaatatatc ctttgtcccc taggatattg ggaagattcg cttcttccga
     1141 taaagtctct aacactttca cgtcatggac acctggattc gtgattgagt aagtaatcgg
     1201 aaagccagtc ttagtaacga tcatatgaat tttaagacca taaaaataag attttttgtt
     1261 gagttatagc caactttggc tatttgattc aatagttttg cttgtctatt tctaactggt
     1321 ttacataatg gactaggaaa actatctata attccgacta gttcaccttt ggtgagcttc
     1381 ttgatgaaaa agtatcgaat aatcttgata gtgtaagcta agtttgaact taagcgagtg
     1441 aatcgactcc tactaggaaa gtcaccatta ggaaataaga cggaacaaac cgctctatac
     1501 gtggctcttt ggctagtata gccattaatt atgccccata taacacaagc aataatcact
     1561 gtatcgcgtt gcttcagttg atcagtattt cttcggtttt taatcttatc tggtacacaa
     1621 ttgtggtata tgttagaaac agttctcata atttccttga atgttataaa agtatctgta
     1681 taatgttcag tatattttga tgaacgcata tgtagtcctc atttcttggg agttttgaga
     1741 ctactatatg cgtttttttg ttgatttacc aacatgttta gacattttcc tctaagaaaa
     1801 aattaactag caccacgggt aagagtatga aaaattttat gatagactta tttattcttt
     1861 gcaattgccc aaaaaattaa accgtcaatt acaaaagact tatgaaacta aatcaactga
     1921 agaatgggag agttattgct agatttttgg tagaagtaac aaaaagtgga catattatca
     1981 taatgactcg ccgaaagtct gataaaaaat tggctaaaaa tattgctgaa tgtttaataa
     2041 aactatcagt tttagctaaa tagttagtag tttacacaaa aaagcattta tgttttttgt
     2101 tgcaaagcaa caatttcatc ttcggtgaaa taagcgaaac tttacaaaaa gtggctagca
     2161 tttgctagct ttttttgtat tgtggagcgt gggttttgag ggggattcaa gggggagctt
     2221 gcgccccttt gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata
     2281 gagcttgcca agccctttag tgagctttag tgagctttac ttattgtacc tttttaggta
     2341 caatggtatt gcgaacgata cgtccgtagg acgtactgaa agggtgggac ttatgagcga
     2401 acaaaatcaa tatttggcta gcaatcattc taaaaaatat acctatcgat ccgagccaaa
     2461 acaaatcagt tttcgagtga gcgaatccga atttgcaaag ctgaagcagt cagctgaagc
     2521 tttgcaaatg agtgtgccgg ctttcgtgaa agccaaggca caaaacgcgc gtctggtgac
     2581 accgaaagtg gcgccagaca ttgctcaggc aatcgcgcgc gatttagcca aagctggcgg
     2641 gaatatcaat caaattgcca agtggtgcaa cacacaccaa cacgacgttg atcctggcga
     2701 cgcccaacgc ttatccgaaa acctaaaaat catgcaaaag gagctacaaa agatatggca
     2761 acagttaaag taagccgaac cacttcttgt agccgagcga tcaattacgc cgaaccacga
     2821 gcaaccgtca aaacagggat aaactgtgac attgattatg ccaaaagcga aatgaaacaa
     2881 attcgcatgc tgtatggaaa agacgatcgt gtacaagcac acttattgat ccagtctttt
     2941 cgaccaggag aaattaccgc tgaaaaagcc aatcaactag gggcagagta tgcgaaaaaa
     3001 atagcccctg accaccaaat cgcgatttac acacatacag acaaagacca tattcataac
     3061 cacattgtca ttaactccgt caatctggaa actgggaaca aatttcaagc ccatggtcaa
     3121 gcatttttaa acaaatgtta tgacctaaat gacgagattt gtcttgaaca tgacttaaca
     3181 atcactgaac gagggaagaa acccgaaaaa cgaaccatgt ctgaaatcaa attaaaagaa
     3241 aaaaatgaac ctgtttggaa agacgaaatt cggtttgcga ttgatcaaac aatgaaaaac
     3301 aaaaaaactc gaacgtataa ccaattttgt gattctctta aaacattcgg cattcattgc
     3361 ttgaatcgtg ggaaaaattt tacttacgaa ttgatccaca aaaagaaaaa agtacgatcc
     3421 aacaaactag gaaaggatta cgaaaaggag acgattttac atgagctgga cagacgagaa
     3481 cgagcctaca atcaacaacg aaccaacgag ctacgaagag ctcgagctag cattcgcgaa
     3541 actgcaaaag aagtacgaag agaacaacga acttttacgc ctgaacaatc ggaaattaga
     3601 ccgactcgaa gaaaccatga acaacacgtt gaacgaggaa cagaccacca acaagaatta
     3661 tcgctctaaa acgttaaaag acatcgaaaa cgaaataaag agacaacgtg cctttcttgc
     3721 gccttatacc caagaaagga tacaaactac gatggattca caagtcaaaa aactccacca
     3781 agcgctatta gaccagcgaa accaagaaca aaaaaaggct ttaattcaac gatcgatgga
     3841 tattcttgcc cccttatgtt gcttagtact aacattatta gccttttggt tggtatgggg
     3901 agaataaaca gacatggcat tctatttacc ctatctactc atttctgcca gtattttagg
     3961 aattatttgg cttagctata aggcttatca aacaagatat tcattaaaag gctctaaaat
     4021 tcgatttaag cgatttttat tggtcggggt tatatttgcc ttatcaatcg ttttaagtgg
     4081 tttattcggc gttttagagg gtaataaacg tgtttcaaga agcattttat taggggatat
     4141 cactcaaaag tatgaagcag catgtgataa aaagaaaaaa gagcaagcat tagcacaaaa
     4201 aatggaggaa tttaccgctt gttatgagga catgaacaat atttttgtgg atcaagagaa
     4261 acgtttaaca gataaaaaca tgaaaaaact cactcgtttt tatcaaaact taccagaaaa
     4321 acagcaaaaa gaggtacaag aaaaatatga gcaagtaaaa aaagacgtgc aatattttaa
     4381 agatacgcag acagaagaag cttgttgtaa cttatttagc gatacaatcc ctttttcaac
     4441 cagtgaacaa gaacgaaaag aacggcaaca atcggtcaca tacgaacgtt atgaaacact
     4501 gcttcagcaa gcaacaaaca tacaaaatcc aacgaaaaaa gaaacggcat taaattatct
     4561 agaaagtgtc aaggaatggt tggatcagca acaacaaaat tgaatttgat tagcaatcat
     4621 gttaaactga acctgcaaat gatcaaacat tagatacacg aaagccccag agaggtcgga
     4681 ttctctgggg ctttttttca ggttgatgtc gcctttattg attttacttc ttgtttcggt
     4741 tattcaacca ataactgaat aaagagacac aaacttctac aagaaggggg caaatgaacc
     4801 gttcaaacat tagatacacc tccttccagt cgcaagactg tcggataggc gacaagttat
     4861 tataccttac aaaataaaat ctgtgtggtg tttttaagta tcccttattt tcgggttgtt
     4921 agggcttgcc ctgaccgcct gtaaggcgct ttaagaacaa gataaggttt tttacccaag
     4981 actgtcttaa aacgacttag acgagctatc agaggcttcc cccacctccg ttactgactt
     5041 gtttttagca aaggagcctt taggcgactg cgcagtacaa tgtgagagca tagcgatcac
     5101 acttttttag cgaccgtagg gagcaaggtt ttgaattggc agttttgaaa aatgcgcgaa
     5161 cctgttggta gaaaaaagaa gtggttttta ggcgggaaat tttgtcaccc tcttttataa
     5221 tctcttttta aacctttttt taaacctctt taccgtcggc tgtgtcctta ctccttcaat
     5281 ggatacagcg ttttataaac tacattttgt ctgcttttaa actacatttt gtctgctttt
     5341 aaactacatt ttgtctgctt ttaaactaca ttttgtctgc ttttaaactc tttttttgca
     5401 ctcacgtatg ttataatatg tattatcaca gacaaaacgt tgtttataac gtctgtatcc
     5461 attgaaggag taaggaggaa tatctttggc aaatgaaatc gtgaaatacc atcatgaatt
     5521 aaataccatt ccgttgcgga aatttacacc agttgaaatg aatctatttt tctctattgt
     5581 gtctagaatg cgcgaaaaag gcgataaaac agtccgtctt acttttgacc aactaaaaga
     5641 tttaagtaat tataaagcaa ctgctaataa tcgtttcatt aatgatttag aaatgactta
     5701 tgataaatta atggatttac gttttggtaa aagaagtgct aatgggctcg atatagatag
     5761 attcgttatg tttagtgagt ttaaaatacg tggaactgct aaagatccat atgtagacat
     5821 acaagtttac gaaaaagctt tacctttact aaacaattta gatgagtggg ttcgctattc
     5881 actccaacaa tttaacgaat tacaaagtag ctatgcaaag actatgtttc gattactaaa
     5941 acaatatcga actaaaggtt ttgcttattt ttctaaagag gattttcatg aactattaga
     6001 tattccaaaa agttactgga acaaccaagc aaacgttgat aatcgtgtta taaagcctat
     6061 acgacaggaa ttaaccgcaa tcttcaaagg attatcaatc aaaaagaaat acggaaaagg
     6121 gcgaggaaaa cctgtcgttg gctatcaatt tacgtttaaa ccagaaatca aaaaagcaga
     6181 tgattttaat aaacatgcac caaccaaaga agaacaaaat aaagcattgg ctttagctga
     6241 taaacaaaaa gaatacgaag aacctgctac aacaattaac gaaaaaatca aacagaagaa
     6301 acgagaactt gaactcctcc aacaaatcaa acaactagaa aaagagaaag aagctcttga
     6361 aaaacaaaca gagtccgcac tttcgaaaaa agagaaaaaa agtttattat ctgatttaga
     6421 taatttattc cagtagcaac ctaggaggca accagtatgc aaccacaaga aacctttaca
     6481 ataaaggaat tgtcagaact ttttaagatg agtaggcaag caatatcaaa acatatccaa
     6541 aaattagatt cttccatgat agctaaaaat gagcgtggtt ataaggttgt attgcgttct
     6601 ggagtattgc aacttgctcg caacttaggc aaccaaatgt tgctagatag gttgcacgag
     6661 tacgaacaag acaacgataa aaatgttaat ataccaagca aaaacgtggt tacaaacaag
     6721 ttgctagatc agttgcgtga agaaaatgaa tttttacgag aacaacttgc cctcagagag
     6781 caaaataatt ctcatataca aaaactactc gaccagcaac aacaactcac cctacaagcc
     6841 aacaaacaaa tcgaaaaact gcaagaacaa ctacagttga cttatacaga agaagctcca
     6901 gagaatgact caacgactct ttctgaagca acagaaacaa tagacaaaca aattgatact
     6961 gaaaaaaaat ggtggcaact atggaaataa ctggagggaa cattcatgac aagaaaacaa
     7021 cttctattcg gttcgatttt actaggaatt ctcggtttca gtccgatgtt attgtcagtg
     7081 atcaataaag ggaataaacg tggtgaatta aaattgaatc caaattatta caacagtcct
     7141 aacgaagaat tgtttgtaaa agaaacggat aaataataaa aaagactgct cttccgagca
     7201 gccagttaaa aaataatgag agtttattct tgaaaaaagc aacgcgctta taaagcgcta
     7261 ccaaaaattt atcatatttt atgaaaaata acaccccagt aaaaaatgac tggtaaaaat
     7321 tcacaaagaa acggaaagaa gcaactgcag aaaatcaagc cttaaaaaga gctttttacc
     7381 tagaccaatc caaaatcaca aagtgagccc ctgtatcggt tgatacaagg gctacttggc
     7441 atactcgaca ttatgtcaac tatgcaatta aataactaac tacttcatat tagcttaata
     7501 aaataatcaa aatcactagg ggaaaaaata ttgtataaaa aaatttttca ttttttaaat
     7561 aagaatagta aatataaaaa taatttaaga tatctattac tattaatcca gatagagcaa
     7621 accaagtaaa tattccgtgt gtaaagctaa aaaaattcat ttgaatcaat tcaaataaac
     7681 ctaatatagt aacaaatata ttaaaaataa tagctgtcaa taccagtaaa ttattttgtt
     7741 tattttttaa attatcttca tttaaaatag aatttatgtt agtactataa aattcacaaa
     7801 ttttttttag tgtctctata tctggtgttg ttttgttatt ttcccacttt gaaatagttt
     7861 gacgtgtaac attaaatttt cttgccacat attcttgagt ataccctttt ttcactctta
     7921 attctcttag cttagatcct aaatcctcca ttttttctcc tatgtaaacg attatttaca
     7981 taaaatttta tcataaaaat actaacgcgc caaaaaagtt ctttatttta taaaacacac
     8041 taaaataaaa taattctagt aaaaaagata ggagaaataa gttatgaaaa aatttttatg
     8101 tctttctgct ttaatgggtg tcttattaac tagtggagga atagttagtg ctacagaagc
     8161 tttaaatcta gatgtacccg aagaccatac cgccatttac ggaggaggaa tggaagctac
     8221 agaagaagga ttttcttcta gtaaattgag atatgctgct ggcggcggag atttcaactg
     8281 tggggttaat ggatttaaag tttatgcaaa ttactaccat gctagagcta aacatagtgc
     8341 aacagctaaa aatggacgag gcggtcaagt acgatcagtt cagaaagctg gagtaagagc
     8401 ttatgcaaca tgcaatgcca cacttactgg aaatactggt tggtggaatg tttattaaaa
     8461 aagagagttt aaactctctt ttttatttac atttgaaagg aaattcaaaa tatgttaaaa
     8521 aaaataaacg ctgttttatc actttttgtc tgcattacaa tactattctt ttctactaat
     8581 gttataaatg gtataaagac ttctgaagcg ctatataaaa ataccgctga aattcatatg
     8641 caatataaag atgatatgga ctttaacagt tatataaacg ctttgtatga aatatctaca
     8701 caagaaaata ttaatatttc tcaatatagt tttactgctg ttaaccaact aactattctt
     8761 gctacaaacc ctaacgctaa caaacactgg aaattttcta aaaaaaatat attagcaaga
     8821 aacgattcta aagtccatta tactaataac aaaaattcta agtaccaatt aaaacttcca
     8881 aataattttt taaatataaa tatttatcct ttttctcatg tggaaaatat tggattaagt
     8941 gaaatattat atgtacaagg aaactcaaac aaattaattc ctgtactgaa aaaatatgga
     9001 acacctacta tttccaatat caatcaatca gatacttttc aaattaatac taatattgtt
     9061 cttgttatct gctatcttag tatatttatt gtaattacta caattatatt tgctttttca
     9121 aaaatgaaag aaatcactct aaaaaaaatg ttaggctata actcttttga tactataaaa
     9181 gattctatta aagagttaaa cctcttattt tgttctttga ttataacttt cattttatct
     9241 atttttttga gcatatatta cagtaacgaa aattttttcc tatcatttct tctatgtagt
     9301 atttttcatt ttataattag ttttttattt atcatagtta ctctaagtat ctatcattta
     9361 tctagtataa aaaattatct aaaaaattca cgaccactta agttaaatct ttgtatatta
     9421 aatatctgtc tattcttaag catgatcctt ctgttaatag cttctacaaa agtaataaat
     9481 atccataatg aagccgaaaa taattctctt aaatattggg aaagaactac taatttatat
     9541 aaaacaaata ttactaatca attaaataga aacaacaccg tagaagaaaa taactacttg
     9601 aaaaaagcta gtaaatttgt ttataaaata cagaagaatt ataaaacttt tattatcgcg
     9661 ccatacaatt acgcaactat ccaagaaaat aataaagaat tttttattgg acaaaaagtt
     9721 tatccaaatt ttgaagacta tgttagtcaa cctgctggtg caggaatatg tgttgattta
     9781 aactatctaa aatattacaa tcctatttct tttgaagaat ctactgactt aaatttaata
     9841 aactctgatc cactaactac ttacattcta gtacctaaaa aatataaaga atatgcaaaa
     9901 atgattgaaa aaaattattt agaagacata cagtttagac ttgaagactc tcctcctcaa
     9961 gctccttata aaactcctaa tttgaaaaat ttaaaaataa aactaatatt tgttaataat
    10021 aaccaaaagt attttagttt taatacctta tatggaaagg caaaagataa ctatacaata
    10081 actgatccca tagttagagt tattaatcca gaaataacag aatcattatt ttggggaaat
    10141 atattgacta gtgatggtgg attatttttt gatcagaaac atacttcaaa ccaaaacttt
    10201 tttaatcaaa ttaatccgta tataaaagaa tttaacctag aaagtatcat aaatttttct
    10261 tattctgttt ttcaagaaac tggtgaatta ttatctcaaa aaaaagtaaa taaattaaat
    10321 gcatatctac aatatctttt aatattagga ttatttataa taattaattt tcaaatgatt
    10381 tttgttagtt ttactataaa ttcaaaaaaa atagctttaa aaaaaatatt tggatattca
    10441 ccaataagtc aagttttaga tttatgttta ctccctttta caatagagtt atttgctact
    10501 atgtgttttc aattgatatt ttcaaaaaac acttacttgt attttataat ttttatgatc
    10561 tttttaataa atctaattac ttctattatt atttataaaa aaaatagttt
//
