LOCUS       CP063018                3777 bp    DNA     linear   BCT 29-OCT-2020
DEFINITION  Klebsiella pneumoniae strain CriePir99 plasmid unnamed5, complete
            sequence.
ACCESSION   CP063018
VERSION     CP063018.1
DBLINK      BioProject: PRJNA580263
            BioSample: SAMN13153742
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Shelenkov,A., Mikhaylova,Y., Yanushevich,Y., Samoilov,A.,
            Petrova,L., Fomina,V., Gusarov,V., Zamyatin,M., Shagin,D. and
            Akimkin,V.
  TITLE     Molecular Typing, Characterization of Antimicrobial Resistance,
            Virulence Profiling and Analysis of Whole-Genome Sequence of
            Clinical Klebsiella pneumoniae Isolates
  JOURNAL   Antibiotics (Basel) 9 (5), E261 (2020)
   PUBMED   32429555
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Shelenkov,A., Mikhaylova,Y., Yanushevich,Y., Samoilov,A.,
            Petrova,L., Fomina,V., Gusarov,V., Zamyatin,M. and Shagin,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2019) Department Of Molecular Diagnostics and
            Epidemiology, Central Research Institute of Epidemiology,
            Novogireevskaya str., 3a, Moscow 111123, Russia
REFERENCE   3  (bases 1 to 3777)
  AUTHORS   Shelenkov,A., Mikhaylova,Y., Yanushevich,Y., Petrova,L., Fomina,V.,
            Zamyatin,M. and Shagin,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-SEP-2020) Department Of Molecular Diagnostics and
            Epidemiology, Central Research Institute of Epidemiology,
            Novogireevskaya str., 3a, Moscow 111123, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8-beta
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 105x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/13/2020 18:40:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.13
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,516
            CDSs (total)                      :: 5,392
            Genes (coding)                    :: 5,268
            CDSs (with protein)               :: 5,268
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 58 of 124
            Pseudo Genes (incomplete)         :: 68 of 124
            Pseudo Genes (internal stop)      :: 27 of 124
            Pseudo Genes (multiple problems)  :: 25 of 124
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CriePir99"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="Russia: Moscow"
                     /lat_lon="55.76 N 37.62 E"
                     /collection_date="2017"
                     /collected_by="Pir Nat Surg Centre"
     gene            190..507
                     /locus_tag="GJJ02_027560"
     CDS             190..507
                     /locus_tag="GJJ02_027560"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QOU65317.1"
                     /translation="MSKAKEKIDNRMAYHLQQLAQLRAKKKEELSKEKKAEKAKRASKN
                     ILFGASICKVLGIDYTKIDEYLPSVIGYLDLKKQNLRNSVYADRGNELLESWKNNGDRN
                     E"
                     /name="hypothetical protein"
     gene            complement(659..958)
                     /locus_tag="GJJ02_027565"
     CDS             complement(659..958)
                     /locus_tag="GJJ02_027565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QOU65318.1"
                     /translation="MQKSKRKLGFLRGKGSIPDQKQLEEMDQEVSSQFFRDLMESAQEA
                     VEIHQGKRSAARRTLYKVNPVTKSVIERSEAGTDVFTATDAPDLFKKLKMDRDN"
                     /name="hypothetical protein"
     gene            1240..2097
                     /locus_tag="GJJ02_027570"
     CDS             1240..2097
                     /locus_tag="GJJ02_027570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QOU65319.1"
                     /translation="MIIQDNHTIEIFKNQLPVKPYCSNNLENGLSIRNKNKALEMLYLQ
                     ANQPAIQTCLLFDLDEDNAFYKFDEVGLPVPTFITKSPESGRCHYGYMLKAGVCKTQHA
                     RLKPLKYAAAIEAGIAGKLASDRGYAGLITKNPLHEHWSPYWSGADLYELDYLADFVEL
                     QTPTKEVKSENYGLGRNVNLFEDLRAYAYKRVLKFKQDLNYEAWERDVLNVAEGLNTHC
                     NALNPLQYNEIKATARSVSRWTWKHFDSATFSDIQSNRGKRNLGKTKRTSAKNKIDNVL
                     ELFK"
                     /name="replication initiation protein"
     gene            2094..2363
                     /locus_tag="GJJ02_027575"
     CDS             2094..2363
                     /locus_tag="GJJ02_027575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="QOU65320.1"
                     /translation="MIINGKKVKAKELAEMAGVSRRTVVKYYAMPREAYEDEAAERRKK
                     AYELREKGLKWKDVGKELNTSLHGAVALYKRYLKLDLNQQQQQQ"
                     /name="HTH domain-containing protein"
     gene            complement(2380..3735)
                     /locus_tag="GJJ02_027580"
     CDS             complement(2380..3735)
                     /locus_tag="GJJ02_027580"
                     /inference="COORDINATES: protein motif:HMM:NF015354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QOU65321.1"
                     /translation="MAIYSFQCKIVSRTKRLNNAVAHAAYINRIDLDNSRDGSKWRYNH
                     RHDDVYKSAVLLPDNANKKFSDPAYLWNEVEKKENRSNSRLARSFIIALPNELTPEQNK
                     NLIEDYIKNHMTSKGMIVNYAIHIDDTNNFHAHLMSTTRELDPNGLEFAKKNVIGRTWN
                     DDDFLEGLRKGWADTANTHLARLDHQVRIDHRTLSAQRDEALEQFDMATTAEAKAEALA
                     KAVSLDRPAIGRIWRSKLNTEEGRALREAQQDRRDVQLVVAEETKKSVLSLPQEIVLNN
                     FTPKKIKSKSKSKTTIEKTRPAQEAVLKTADTGGEIKDLKYSSFNKFLLDIKIRYNKHQ
                     KNNATKKLNKKIDKIIKTIDREEKKSNIMPNHYNSDYFIDDKSTIIKDEYKDDEYKKLQ
                     KFFDDVNKRGVELKNKKEAILKAEQENKKMLSQQPKHNPKNDDQNDRHIKYK"
                     /name="MobA/MobL family protein"
     Typing          1241..2047
                     /name="rep_cluster_1451"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.916"
                     /coverage="94.0"
                     /evalue="0.0"
     Typing          complement(2384..3735)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="84.035"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gggaccacta caaactttag tttgtgtaag tgggcattgc tgttttattt tttcctcgct
       61 ttcgctcgga ctaaaataag caatgtcgct ttacttatat ttattaaata acatataaaa
      121 atacttttga caaatattta taaatatatt attgaataaa cattaaaata aataatagga
      181 gcttaatata tgagtaaagc aaaagagaaa atagataata ggatggctta tcatcttcaa
      241 cagcttgcac aactaagagc aaagaaaaaa gaagagttga gcaaagaaaa aaaggcagaa
      301 aaagcaaaaa gagcaagtaa aaatattttg tttggtgctt ctatctgtaa agtgttaggt
      361 attgattaca ctaaaataga tgaatatttg ccttctgtta ttggatattt agatttaaaa
      421 aaacagaact taagaaatag tgtgtatgcc gatagaggca atgaactttt agaaagctgg
      481 aaaaataacg gcgacagaaa cgagtaaaac ttgcttttgc tcgtttgctg gcgtctatgc
      541 agttaggtta gaaatggtca taaaatcaac ggagtatagt atatattagt atagtatata
      601 ctgccctccg ggatagctgg ccagcttcgt aacttggtgg tttgtttggt ggaggtggtc
      661 agttgtccct gtccattttt agctttttga agagatcagg ggcatcagta gctgtaaaaa
      721 cgtctgtacc ggcttctgag cgttctataa ctgatttggt gactgggttt accttgtaca
      781 gcgttctacg agctgcagag cgtttacctt gatggatttc gacagcttcc tgtgctgatt
      841 ccatcaggtc acggaaaaac tggctgctga cttcctgatc catctcttcg agctgctttt
      901 ggtcagggat agagccttta ccacgtaaaa agccgagctt acgctttgat ttctgcatct
      961 catgttctcc tatccagcac agccagatct tactctactc tctattttat cagccttcgg
     1021 ccttgttggt ttattggaat aggtttccag tagtgccgga aaactgcccc tttacagggc
     1081 tgttatctga tatggttaag actcccactt gacaaacttt ttagatcggt taaaaccgtt
     1141 acagagttga aaaatataac aaaaacaaaa accccgcatt ctgctctatg tttggcgaca
     1201 tacagaaggc ggggccagat aaaaaaggac tgcttaaaaa tgataataca agataaccat
     1261 actatagaaa tatttaaaaa tcaattacct gtgaagcctt actgttccaa taatttagaa
     1321 aatggtctta gtatcaggaa caaaaacaaa gcacttgaga tgctttacct tcaagcaaat
     1381 cagccagcta tacagacgtg cttactgttc gatctcgatg aggacaacgc cttctacaag
     1441 ttcgatgagg tagggcttcc tgttcctacg ttcatcacaa aatctcctga gtctggacgt
     1501 tgccactacg gctatatgct caaagccggt gtttgtaaga cacaacacgc aaggctgaag
     1561 cctttaaagt atgcagcagc catagaggct ggcattgctg gaaagttagc ttctgatcga
     1621 ggatatgctg gactgattac aaaaaatcca ctgcatgaac attggtcacc ctattggtca
     1681 ggtgctgatc tgtatgagtt agattatttg gctgattttg ttgagttaca aacaccaaca
     1741 aaggaagtta aatcagagaa ctacgggctg ggacgcaatg ttaacttgtt tgaagattta
     1801 agagcttatg cttataagcg agtattgaag ttcaagcagg atttgaacta tgaagcgtgg
     1861 gaacgtgatg ttttaaatgt agctgaagga ctcaatactc attgtaatgc tttaaatccg
     1921 ctacaatata atgagataaa agcaactgcg agatctgtta gtcgctggac ttggaagcat
     1981 tttgatagtg ctactttttc agatatacaa tcaaacagag gtaaaagaaa cttaggcaaa
     2041 acaaaaagaa ccagtgctaa aaataaaata gataacgtat tggagctatt caaatgatta
     2101 taaatggtaa gaaagttaaa gctaaagagc tggctgaaat ggctggcgta tcacgcagaa
     2161 cggtagttaa gtattatgct atgccgcgtg aagcctatga agatgaagca gcagaacgta
     2221 gaaaaaaagc ctatgagctg agagagaaag gcttgaagtg gaaagatgta gggaaagagt
     2281 tgaatacttc tctgcatggt gctgtagctc tttataagcg ttatttgaag ttagatttaa
     2341 accaacaaca acaacaacaa tagcaggttt acggcctgct tatttgtatt taatgtgtct
     2401 atcattctga tcgtcatttt ttgggttgtg tttaggttgt tgtgatagca tctttttatt
     2461 ttcttgctca gctttaagta tggcttcttt tttatttttt agctcaacac ctcttttatt
     2521 cacatcatca aaaaacttct gtagcttctt atattcatca tctttgtatt catctttaat
     2581 aatcgtactt ttatcatcaa tgaaataatc actattataa tgattaggca tgatgttaga
     2641 ttttttctct tctctatcta tagtctttat tatcttgtct attttcttat ttagcttttt
     2701 agtggcgtta tttttttgat gcttgttata tcttatctta atatctaata aaaacttatt
     2761 aaaagaacta tatttgagat cttttatctc tccccctgta tcagcagttt ttaaaactgc
     2821 ttcttgggcg gggcgtgttt tttcaatagt agtttttgat tttgattttg attttatttt
     2881 tttaggtgtg aagttattta aaactatctc ttgtggtaaa gacaaaacac tctttttagt
     2941 ctcttcagca acaacgagct gcacatctct tctgtcttgc tgtgcttcac gtaaagctct
     3001 accctcttcc gtgttcagct tactacgcca aatcctacca atagcaggtc tatcaaggct
     3061 tacagcctta gccagagcct cagctttagc ctctgcagtg gtagccatat cgaactgctc
     3121 aagagcttca tcacgttgag ctgacagcgt tctatggtca atcctgacct gatgatcaag
     3181 ccttgctaaa tgagtattag cggtatctgc ccagcctttc cttaaccctt caagaaaatc
     3241 atcatcattc caagttctgc cgataacatt cttttttgcg aactctaaac cattaggatc
     3301 taactccctt gtagtgctca tcaaatgagc gtgaaagttg tttgtatcat ctatatgaat
     3361 ggcatagttt acaatcatac ctttagatgt catatgattt ttaatgtaat cttcaatcaa
     3421 gttcttgttt tgttccggtg ttagttcgtt tggtaaagca ataataaaac tacgagccaa
     3481 acggctatta gatctatttt ctttcttctc tacttcgttc cataaataag ctgggtcaga
     3541 aaactttttg ttggcattat ctggaagcaa aacagcagat ttatatacat catcatgcct
     3601 atgattataa cgccactttg aaccatcgcg gctattatca agatcaatac ggttaatata
     3661 tgctgcatga gcaacagcat tatttaaacg ttttgttctt gatacaatct tacattgaaa
     3721 actatatata gccataaatg gaaaccttat ttttttgctt ttgaccttag ccccgca
//
