LOCUS       CP061256                7178 bp    DNA     circular BCT 13-JAN-2022
DEFINITION  Escherichia coli strain STEC310 plasmid pSTEC310_3, complete
            sequence.
ACCESSION   CP061256
VERSION     CP061256.1
DBLINK      BioProject: PRJNA655129
            BioSample: SAMN15748000
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7178)
  AUTHORS   Pan,Y., Xiong,Y. and Yang,X.
  TITLE     Genomic characteristics of Shiga toxin-producing Escherichia coli
            (STEC)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7178)
  AUTHORS   Pan,Y., Xiong,Y. and Yang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2020) National Institute for Communicable Disease
            Control and Prevention, Chinese Center for Disease Control and
            Prevention, No. 155, Changbai Rd, Beijing 102200, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. v. 4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 09/02/2020 12:38:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.12
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,304
            CDSs (total)                      :: 5,174
            Genes (coding)                    :: 4,211
            CDSs (with protein)               :: 4,211
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 100
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 963
            CDSs (without protein)            :: 963
            Pseudo Genes (ambiguous residues) :: 0 of 963
            Pseudo Genes (frameshifted)       :: 781 of 963
            Pseudo Genes (incomplete)         :: 183 of 963
            Pseudo Genes (internal stop)      :: 82 of 963
            Pseudo Genes (multiple problems)  :: 80 of 963
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="STEC310"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSTEC310_3"
                     /geo_loc_name="China: Shanghai"
                     /collection_date="2013"
     gene            complement(525..1681)
                     /locus_tag="H6S13_26060"
                     /pseudo=""
     CDS             complement(525..1681)
                     /locus_tag="H6S13_26060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219937.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /name="DNA cytosine methyltransferase"
     gene            1769..2828
                     /locus_tag="H6S13_26065"
                     /pseudo=""
     CDS             1769..2828
                     /locus_tag="H6S13_26065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034299.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="HindVP family restriction endonuclease"
                     /name="HindVP family restriction endonuclease"
     gene            3808..4014
                     /locus_tag="H6S13_26070"
     CDS             3808..4014
                     /locus_tag="H6S13_26070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UIV35906.1"
                     /translation="MAKLPQEKPKEVIQAGFDDVKKAYKKGAKTVNDTYAEQAGRDLQS
                     GDGELMATYRRNKKAIDKLDAEG"
                     /name="hypothetical protein"
     gene            complement(4398..4594)
                     /locus_tag="H6S13_26075"
                     /pseudo=""
     CDS             complement(4398..4594)
                     /locus_tag="H6S13_26075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565221.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(4625..4879)
                     /gene="mbeD"
                     /locus_tag="H6S13_26080"
     CDS             complement(4625..4879)
                     /gene="mbeD"
                     /locus_tag="H6S13_26080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UIV35907.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQQDNA
                     MLNEQVMQLSQQVQHLSERTERLSERTERLSQLYSENRR"
                     /name="mbeD"
     gene            complement(4886..5404)
                     /locus_tag="H6S13_26085"
     CDS             complement(4886..5404)
                     /locus_tag="H6S13_26085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="UIV35908.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIRDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSG
                     GTLWYLGGMIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            complement(5382..6104)
                     /locus_tag="H6S13_26090"
                     /pseudo=""
     CDS             complement(5382..6104)
                     /locus_tag="H6S13_26090"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(6082..6248)
                     /locus_tag="H6S13_26095"
                     /pseudo=""
     CDS             complement(6082..6248)
                     /locus_tag="H6S13_26095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(6318..6427)
                     /locus_tag="H6S13_26100"
                     /pseudo=""
     CDS             complement(6318..6427)
                     /locus_tag="H6S13_26100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538659.1"
                     /note="frameshifted; incomplete; partial on complete
                     genome; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /name="plasmid mobilization relaxosome protein MobC"
     gene            6468..6659
                     /locus_tag="H6S13_26105"
     CDS             6468..6659
                     /locus_tag="H6S13_26105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UIV35909.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(6738..6822)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.674"
                     /coverage="100.0"
                     /evalue="6.569999999999999e-34"
     Typing          4..248
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.968"
                     /coverage="95.0"
                     /evalue="3.17e-109"
     Typing          6842..7178
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.813"
                     /coverage="100.0"
                     /evalue="3.08e-130"
     Typing          complement(4528..6092)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.146"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(6172..6347)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.915"
                     /coverage="99.0"
                     /evalue="9.96e-32"
ORIGIN
        1 ctgtgcgctc tctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
       61 gggaagcgtg gcgctttctc atagctcacg ctgttggtat ctcagttcgg tgtaggtcgt
      121 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag ccgaccgctg cgccttatcc
      181 ggtaactatc gtcttgagtc caacccggta agacacgaca aagcgccact ggcagcagcc
      241 actggtaact ggacaaggtg gattgagata ttaagagttc ttgaagtggt ggcctaactg
      301 cggctacact agaaggacag tatttggtat ctgtgctcca ccaagccagt taccttcgga
      361 agaaagagtt ggtagctcta gattccggca aacaaaccac cgttggtagc ggtggttttt
      421 ttgtttacag gcagcagatt acgcgcagaa aaaaaggatc acaagaggat cctttgatct
      481 ttttatcccc tccccctccc ctgagagaca taaattcgta tttattacct tcgacaatat
      541 tcgtaatata atttaaaagc ccttcttaac tgagacttca ggctaacccc aagcctgaca
      601 aacgattcaa gcctttccag tttcataata attcatctat ttcacagtta taaaaatcca
      661 ccccctctga atcaaaaaat gaaacacctc tgcaacatct agatataata tcgctagagg
      721 ttctttttgt tagtttcttt tcattcagca accacgccct aaagccttct ttatcataaa
      781 taccttttac ttgttttttt tcatactcca tgattgtcac cgcaacatat tttgctaact
      841 caaccggaac agcattacct atcatttgct ccaaatttgt ttttgcacct ttaaatttaa
      901 agtcttcagg aaatgtttgc aatcttgctc tttcaaaggt tgttaatggc cttacattct
      961 cagaaatact gacaggatcg cctgcatgcc ctaaatatcc atcaggtatc ggtctattaa
     1021 cccctcttac tgttggcgca ggttcatcaa tagagaaaat agcccttctg ttataatttc
     1081 tcggatgtct gtaataatac tcaatcccca atgaatcacc aagataattt ctaactgtca
     1141 tactatcttt cgatatcccg cacatcactt cgttaaggat aaaaccatcc ctcacgtcaa
     1201 gctttccaat tacaaataat ctttttcttt tttgcggaac accacactta ctggcatcca
     1261 aaacaatttc tgttaaccca taccctgatt ctttaaagat tcctcgagct tctaaatagg
     1321 caccacttct taatgcccga tctacatttt ccatcacaaa ccactttggc tttattcttt
     1381 ttataatttc tgaaaatgag cgagttaagt tagctctagc accttcaatt cttaaacctg
     1441 catgactgaa gtcttggcaa ggaggccctc caataatcat atcgaagtct atatctttaa
     1501 gctccctcac cacatcatca acattgctca aatctagttt ttttatcgga tggctaaaat
     1561 tatctctgta acattcaata gcaggatccc agaaatcata agcacctaca acatcaaacc
     1621 cccccttcaa aaagcctagg gataaccccc cacacccact aaaaagatca attattctca
     1681 taacataaaa aactcattat catatcactc cacatcatga tatagaaaca caccatagac
     1741 gtcaataaat tgacgtcagg agcaaaaagt gagcaaaata gaatctcttt atggtttaaa
     1801 aacaccaaca ggaaagagag cgatctgtgg ggaaagaacc aatttaacag ttcttttccc
     1861 acctctctag catgttatat gagagacaac aaaatgaatc cgatttatct ttatttagat
     1921 aaggatttaa aagtgcatgc caaagaagta tcttttgact tcgtttttaa cacaacatta
     1981 aaaaatagtg atcttgaatt tcaatttgag aaaaaatatg aaccatacca acagtatgct
     2041 tatgatgaca taaaaggaat agatctagtt ataaaaggaa aaaatagttt tttaaaagca
     2101 ctagaaataa aattaaccgt acttccagat agcacgacat ataaagaaaa cgagagcgaa
     2161 tggagttcag agattgtaat aagacctgca agcacttctt attgtgccct tggcataatg
     2221 cactcttgca gcgataaatt ccatgaaata agaaaaatat ttgaatccac atgcgaaagc
     2281 atacagcact ggggaatgaa actgaagttc tttcaaagaa agaaaatata ttaaattgcc
     2341 taaacacttt ccaagaaaat tttcttgatt accagaaacc ttttttaatg caaccattat
     2401 ggaaaacaaa aggcaagagc ccaatattag atcaaaatgc ctttgacata ttcatctgga
     2461 gtgattttgc tttgtgtcga acctttattg atagatcctc atataacaat tcatctgcca
     2521 gcagactaat gagatcatca gccagactcg ccagaatggt gtatgaaatt gcgagcaaaa
     2581 acaagacaaa catcaataga atttatactg agatgaactt tggatatcaa agtgataaag
     2641 agttctcgct aaacggaaaa ataacaaaag cttatatgga tcacccaaga agaacaaaac
     2701 ccatcctccc accatcagta ttaaaagaaa taattttaaa tggcgggcaa aataaattaa
     2761 gcccagagag acgctttgat caaagcgttt attatactgc atctcacctg tttgatgaaa
     2821 taaagtaatt tttcttggtg gctcgccagc cctgaattcg cccatcaaaa atcagtagcg
     2881 gaacaggcat actagacatg ctcgctttta cggcgagcat cttgacggag tttgagacgc
     2941 aatggaacag caaccaactc tatgcatcac aagctcaaaa atgattacca tgcattgaac
     3001 tctagttacc tttgccaatg attgctcctg gttgcttcat gcccgtaaag gcaatttcga
     3061 cggtcattac attgaacatg gggtttgagg gccaaccgta cgaaaatgta cggtaagagg
     3121 aaaattatcg tcttaaaaat cgattagtag acaatgccgt ccgttaagtg ccaattttcg
     3181 attaaaaaga caccgttttg atggcgtttt tctatgtcta ttatgtcttg atatatcata
     3241 cagttacttc actaacgtac gttttcgttc cattggactt cagaccccaa cataacatct
     3301 cgaccgcgta ctgaacggtc tcagccggat cttcgaaccc atctatcgta gaattgatac
     3361 tctacctctg tgactaacaa gacagttccg ctactcttct gagtattcat tcatcaaaaa
     3421 gcaggatatc cagtgtcaaa cattgttcat ctatctgata aaaaacaaaa aaacaaacca
     3481 gttgatattt cttttgatga ttacacagac aatctcagca aagggttcgt caaaaatctc
     3541 agcaataaac aaaaaaagaa ttaattagta aaatcaaagc gatacttaaa tcgaggaatg
     3601 tagcgttccg ttcgacgccg ccctctgatt ggaaagagaa aagtagaacc tttgcaattt
     3661 tctttcctga aaaaaaggtt gtttttacat taggagtagt gaacaaatta aagatagagg
     3721 acggttggat aaatcataaa atcagcgaca cagaagatgc cgtcttatct gttggaaaaa
     3781 taatcaacct tatagaggat tcacagcatg gctaaattac ctcaagaaaa gccgaaagaa
     3841 gtcatccaag ccggatttga tgatgttaaa aaggcctata aaaaaggcgc taagactgta
     3901 aacgatacct atgctgagca agcaggtcgt gacttacaat caggcgatgg tgaactaatg
     3961 gctacctatc ggcgcaataa aaaagcgata gacaagttag atgctgaagg ttgatctgaa
     4021 aagtcagtct tctgggcgga cttttcgtct ctaagactgg ctgcatattg tatttaccat
     4081 aacatccgtt gcacgcaccg cagaaacgcg ttcggtgcga tcacggcagc agacaggtaa
     4141 aaatggcaac aaaccacccg aaaaactgcc gcgatcgcac ccggtaaatt ttaaccacat
     4201 gcatagctat gcagccatgt gaatcacgca ggaattgcca gccagagact gctgaaacgg
     4261 atttttttca tgctttcgga agcgaagaag acggggaccg gagcgggaaa acagaagttc
     4321 aggggaatga gcgcgatctg gcaatagagg caaaacacag caacaaaaga cacaccagaa
     4381 tcgcgcccgt atgcgtttta acgcgttcct gtgttttcag caaggtctga ctgaagcgcg
     4441 ttcccggtgc ccccgaacca accattatcc caaaaaatgc accggaaacg cgctgacaac
     4501 attttagcct gtctctgatt accatccagc gaagggccat ccagtgtccg ttcctgtatt
     4561 tccagctggc gctcacgctc cagggataac tcatgttcgc gctcctgtat cagccgttcg
     4621 tcccttatct cctgttctcg ctgtataact ggctcaagcg ttctgtccgc tcgctcaagc
     4681 gttctgtccg ctcgctcaag tgctgcacct gctgactcaa ctgcatgacc tgctcgttca
     4741 gcatcgcgtt gtcctgttgc gtaagcgaaa acattttctg caattccacg aaggcgctct
     4801 cccattcgtt cagccgctgc atatagtcct gttgcagttg ctctaaggcg ttcagcaaat
     4861 gtctttccag ctccgtcact ctgtgtcact ccgtcagttg tacccagtcc ttcccctgat
     4921 agggaatcac cgttgccgat ttcccctgcg ggatgaccag aaatttcctt ctcccgtcct
     4981 gcacaaactc cacgccccat gtcttcgcgt tcagtttctg caatgtttct tcctgtatcc
     5041 tgatttcttc caggttcgcc tgtatcatcc cgccaagata ccagagcgtc ccgccactca
     5101 cggtaaacag gaaaatgacc atccccagta acatcatgcc cgtattccct gccagcttta
     5161 acacgtcctt cctgtgctgc atcatcgcct ctttcacccc ttcccggtgt tttttcagtg
     5221 attcctctgt cgaagctgtg aacagggcta tagcgtctct gattttcgtc tcgtttgatg
     5281 tcacagcctt gcttacagat ttttcgagct tgctgaactc gtcgttcagc attgtctctg
     5341 tagattcggc tctctctttc agctttttct cgaactccgc gcccgtctgt aaaagattgc
     5401 tcataaaatg ctcctttcag cctgatattc ttcccaccgt tcgggtctgc aatgctgata
     5461 ctgcttcgcg tcaccctgac cacttcaagc cctgcctctg tgagcgcctg aatcacatcc
     5521 tgacggcctt ttatctcccc gacatgataa agagcgtcta tcccgcgtgt gacgctctct
     5581 gcaagcgcct gtttcgtttc cggcaggtta tcaggaaggg tcagcgtccg gcggttctcc
     5641 ggggcgttgg ggtcatgcag cccgtaatgg tgattcacca gcgtctgcca ggcatcaatt
     5701 ctcggcctgt ctgcccggtc gtagtacggc tggaggcgtt ttccggtctg tagctccatg
     5761 ttcggaatga caaaattcag ctcaagccgt cccttgtcct ggtgctctac ccacaggatg
     5821 ctgtactgat tcttttcgag accgggcatc agtacacgct caaagctcgc catcaccttt
     5881 tcacgtcctc ccggcggcag ctccttctcc gcgaacgaca gaacacctga cgtgtatttc
     5941 ttcgcaaatg gcgtggcatc gatgagttcc cggacttctt ccggatcacc ctgaagcacc
     6001 gttgcccctt cccggttacg ctcccttccc agcaggtaat caaccggacc actgccaccg
     6061 ccttttcccc tggcatgaaa cttaactatc atcccgcgct ccatgttccc tgacgacctg
     6121 ccgtaagctg cgcaactctc tctcgatggc catcagtgcg gccaccacct gaaccggtca
     6181 ccggaagacc actgcccgct gttcaccttc ctcgccgtct gattcaggtt attcccgatg
     6241 gcagccagct ggcgcagtaa cggcggtgcc agtgtcggca gcttcccgga acgggcaacc
     6301 ggctcaccca gacagacccg ccgcatccat accgccagct gtttacctca cagcgttcca
     6361 gtaaccgtgc atgttcatca tcagtaaccc gtatcgtgag catcctctcg cgtttcatcg
     6421 gtatcattac cccatgaaca gaaatccccc cttacacgga ggcatcagtg actaaacagg
     6481 aaaaaaccgc ccttaacatg gcccgcttta taagaagcca gacattaacg ctgctggaaa
     6541 agctcaacga actggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg
     6601 ccgatgagct ttaccgcagc tgcctcgcac gcttcgggga tgacggtgaa aacctctgac
     6661 acatacagct cccggagacg gtcacagctt gtctgtgagc ggatgccggg agcagacaag
     6721 cccgtcaggg cgcgtcaggg ggtttagggg cgaagccctg aaccagtcac gtagcgatag
     6781 cggagtgtat actggcttaa ctatgtggca tcagtgcaga ttataagaaa agagcaccat
     6841 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatccg gcgctcttcc
     6901 gcttcctcgc tcactgactc gctacgctcg gtcgttcggc tgcggcgagc ggagtcggct
     6961 cactcaaagc ggtatacggt tatccacaga atcaggggat aagaccggaa agaacatgtg
     7021 agcaaaaagg caaaggacaa caaaaggccg cgccgctggc gtttttccat aggctccgcc
     7081 cccctgacga gcatcacaga aatcgacgct caagccagag gtggcgaaac ccgacaggac
     7141 tataaagata ccaggcgttt ccccctggaa gctccctc
//
