LOCUS       CP061254               11045 bp    DNA     circular BCT 13-JAN-2022
DEFINITION  Escherichia coli strain STEC310 plasmid pSTEC310_1, complete
            sequence.
ACCESSION   CP061254
VERSION     CP061254.1
DBLINK      BioProject: PRJNA655129
            BioSample: SAMN15748000
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11045)
  AUTHORS   Pan,Y., Xiong,Y. and Yang,X.
  TITLE     Genomic characteristics of Shiga toxin-producing Escherichia coli
            (STEC)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11045)
  AUTHORS   Pan,Y., Xiong,Y. and Yang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2020) National Institute for Communicable Disease
            Control and Prevention, Chinese Center for Disease Control and
            Prevention, No. 155, Changbai Rd, Beijing 102200, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. v. 4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 09/02/2020 12:38:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.12
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,304
            CDSs (total)                      :: 5,174
            Genes (coding)                    :: 4,211
            CDSs (with protein)               :: 4,211
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 100
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 963
            CDSs (without protein)            :: 963
            Pseudo Genes (ambiguous residues) :: 0 of 963
            Pseudo Genes (frameshifted)       :: 781 of 963
            Pseudo Genes (incomplete)         :: 183 of 963
            Pseudo Genes (internal stop)      :: 82 of 963
            Pseudo Genes (multiple problems)  :: 80 of 963
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..11045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="STEC310"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSTEC310_1"
                     /geo_loc_name="China: Shanghai"
                     /collection_date="2013"
     gene            join(10402..11045,1..1485)
                     /locus_tag="H6S13_25080"
                     /pseudo=""
     CDS             join(10402..11045,1..1485)
                     /locus_tag="H6S13_25080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395566.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            1546..1758
                     /locus_tag="H6S13_25085"
     CDS             1546..1758
                     /locus_tag="H6S13_25085"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UIV35759.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="hypothetical protein"
     gene            1760..1966
                     /locus_tag="H6S13_25090"
     CDS             1760..1966
                     /locus_tag="H6S13_25090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UIV35760.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="hypothetical protein"
     gene            1996..2835
                     /locus_tag="H6S13_25095"
     CDS             1996..2835
                     /locus_tag="H6S13_25095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="UIV35761.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="AAA family ATPase"
     gene            2822..3694
                     /locus_tag="H6S13_25100"
     CDS             2822..3694
                     /locus_tag="H6S13_25100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C"
                     /protein_id="UIV35762.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AGCPPPTLL"
                     /name="replication protein C"
     gene            3982..4797
                     /gene="sul2"
                     /locus_tag="H6S13_25105"
     ARG             3983..4794
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             5242..5660
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="52.06"
     ARG             4873..5657
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="98.13"
     gene            5660..6496
                     /locus_tag="H6S13_25115"
     CDS             5660..6496
                     /locus_tag="H6S13_25115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="UIV35755.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            complement(6538..6720)
                     /locus_tag="H6S13_25120"
     CDS             complement(6538..6720)
                     /locus_tag="H6S13_25120"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UIV35756.1"
                     /translation="MSRPSPRRYGASSAFPTLTGLVALAAGLAAVYGPANAPERGRLRE
                     GAESYAKALASDILR"
                     /name="hypothetical protein"
     gene            6883..7125
                     /locus_tag="H6S13_25125"
     CDS             6883..7125
                     /locus_tag="H6S13_25125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="UIV35757.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="relaxase"
     gene            complement(7157..7783)
                     /gene="tetR(A)"
                     /locus_tag="H6S13_25130"
     CDS             complement(7157..7783)
                     /gene="tetR(A)"
                     /locus_tag="H6S13_25130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="UIV35765.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGGTVEQAPLSPLL
                     RAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     ARG             7890..9535
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="96.24"
                     /coverage="129.25"
     ARG             7890..9082
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="96.49"
                     /coverage="99.5"
     gene            complement(9020..9457)
                     /locus_tag="H6S13_25140"
                     /pseudo=""
     CDS             complement(9020..9457)
                     /locus_tag="H6S13_25140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166363.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /name="GNAT family N-acetyltransferase"
     gene            complement(9919..10203)
                     /locus_tag="H6S13_25145"
     CDS             complement(9919..10203)
                     /locus_tag="H6S13_25145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="UIV35758.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C"
     ARG             5661..6493
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          10158..10311
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="2.94e-75"
     Typing          2014..2835
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          11..1482
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          10403..11043
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="9.04e-135"
ORIGIN
        1 ggaaggccgg gcatccgcac cgaccgggca gacgtggccc tgaacatcga caccgccaac
       61 gcccagatca tcgacttaca ggaataccgg gaggcaatag accatgaacg caatcgacag
      121 agtgaagaaa tccagaggca tcaacgagtt agcggagcag atcgaaccgc tggcccagag
      181 catggcgaca ctggccgacg aagcccggca ggtcatgagc cagacccagc aggccagcga
      241 ggcgcaggcg gcggagtggc tgaaagccca gcgccagaca ggggcggcat gggtggagct
      301 ggccaaagag ttgcgggagg tagccgccga ggtgagcagc gccgcgcaga gcgcccggag
      361 cgcgtcgcgg gggtggcact ggaagctatg gctaaccgtg atgctggctt ccatgatgcc
      421 tacggtggtg ctgctgatcg catcgttgct cttgctcgac ctgacgccac tgacaaccga
      481 ggacggctcg atctggctgc gcttggtggc ccgatgaaga acgacaggac tttgcaggcc
      541 ataggccgac agctcaaggc catgggctgt gagcgcttcg atatcggcgt cagggacgcc
      601 accaccggcc agatgatgaa ccgggaatgg tcagccgccg aagtgctcca gaacacgcca
      661 tggctcaagc ggatgaatgc ccagggcaat gacgtgtata tcaggcccgc cgagcaggag
      721 cggcatggtc tggtgctggt ggacgacctc agcgagtttg acctggatga catgaaagcc
      781 gagggccggg agcctgccct ggtagtggaa accagcccga agaactatca ggcatgggtc
      841 aaggtggccg acgccgcagg cggtgaactt cgggggcaga ttgcccggac gctggccagc
      901 gagtacgacg ccgacccggc cagcgccgac agccgccact atggccgctt ggcgggcttc
      961 accaaccgca aggacaagca caccacccgc gccggttatc agccgtgggt gctgctgcgt
     1021 gaatccaagg gcaagaccgc caccgctggc ccggcgctgg tgcagcaggc tggccagcag
     1081 atcgagcagg cccagcggca gcaggagaag gcccgcaggc tggccagcct cgaactgccc
     1141 gagcggcagc ttagccgcca ccggcgcacg gcgctggacg agtaccgcag cgagatggcc
     1201 gggctggtca agcgcttcgg tgatgacctc agcaagtgcg actttatcgc cgcgcagaag
     1261 ctggccagcc ggggccgcag tgccgaggaa atcggcaagg ccatggccga ggccagccca
     1321 gcgctggcag agcgcaagcc cggccacgaa gcggattaca tcgagcgcac cgtcagcaag
     1381 gtcatgggtc tgcccagcgt ccagcttgcg cgggccgagc tggcacgggc accggcaccc
     1441 cgccagcgag gcatggacag gggcgggcca gatttcagca tgtagtgctt gcgttggtac
     1501 tcacgcctgt tatactatga gtactcacgc acagaagggg gttttatgga atacgaaaaa
     1561 agcgcttcag ggtcggtcta cctgatcaaa agtgacaagg gctattggtt gcccggtggc
     1621 tttggttata cgtcaaacaa ggccgaggct ggccgctttt cagtcgctga tatggccagc
     1681 cttaaccttg acggctgcac cttgtccttg ttccgcgaag acaagccttt cggccccggc
     1741 aagtttctcg gtgactgata tgaaagacca aaaggacaag cagaccggcg acctgctggc
     1801 cagccctgac gctgtacgcc aagcgcgata tgccgagcgc atgaaggcca aagggatgcg
     1861 tcagcgcaag ttctggctga ccgacgacga atacgaggcg ctgcgcgagt gcctggaaga
     1921 actcagagcg gcgcagggcg ggggtagtga ccccgccagc gcctaaccac caactgcctg
     1981 caaaggaggc aatcaatggc tacccataag cctatcaata ttctggaggc gttcgcagca
     2041 gcgccgccac cgctggacta cgttttgccc aacatggtgg ccggtacggt cggggcgctg
     2101 gtgtcgcccg gtggtgccgg taaatccatg ctggccctgc aactggccgc acagattgca
     2161 ggcgggccgg atctgctgga ggtgggcgaa ctgcccaccg gcccggtgat ctacctgccc
     2221 gccgaagacc cgcccaccgc cattcatcac cgcctgcacg cccttggggc gcacctcagc
     2281 gccgaggaac ggcaagccgt ggctgacggc ctgctgatcc agccgctgat cggcagcctg
     2341 cccaacatca tggccccgga gtggttcgac ggcctcaagc gcgccgccga gggccgccgc
     2401 ctgatggtgc tggacacgct gcgccggttc cacatcgagg aagaaaacgc cagcggcccc
     2461 atggcccagg tcatcggtcg catggaggcc atcgccgccg ataccgggtg ctctatcgtg
     2521 ttcctgcacc atgccagcaa gggcgcggcc atgatgggcg caggcgacca gcagcaggcc
     2581 agccggggca gctcggtact ggtcgataac atccgctggc agtcctacct gtcgagcatg
     2641 accagcgccg aggccgagga atggggtgtg gacgacgacc agcgccggtt cttcgtccgc
     2701 ttcggtgtga gcaaggccaa ctatggcgca ccgttcgctg atcggtggtt caggcggcat
     2761 gacggcgggg tgctcaagcc cgccgtgctg gagaggcagc gcaagagcaa gggggtgccc
     2821 cgtggtgaag cctaagaaca agcacagcct cagccacgtc cggcacgacc cggcgcactg
     2881 tctggccccc ggcctgttcc gtgccctcaa gcggggcgag cgcaagcgca gcaagctgga
     2941 cgtgacgtat gactacggcg acggcaagcg gatcgagttc agcggcccgg agccgctggg
     3001 cgctgatgat ctgcgcatcc tgcaagggct ggtggccatg gctgggccta atggcctagt
     3061 gcttggcccg gaacccaaga ccgaaggcgg acggcagctc cggctgttcc tggaacccaa
     3121 gtgggaggcc gtcaccgctg atgccatggt ggtcaaaggt agctatcggg cgctggcaaa
     3181 ggaaatcggg gcagaggtcg atagtggtgg ggcgctcaag cacatacagg actgcatcga
     3241 gcgcctttgg aaggtatcca tcatcgccca gaatggccgc aagcggcagg ggtttcggct
     3301 gctgtcggag tacgccagcg acgaggcgga cgggcgcctg tacgtggccc tgaacccctt
     3361 gatcgcgcag gccgtcatgg gtggcggcca gcatgtgcgc atcagcatgg acgaggtgcg
     3421 ggcgctggac agcgaaaccg cccgcctgct gcaccagcgg ctgtgtggct ggatcgaccc
     3481 cggcaaaacc ggcaaggctt ccatagatac cttgtgcggc tatgtctggc cgtcagaggc
     3541 cagtggttcg accatgcgca agcgccgcca gcgggtgcgc gaggcgttgc cggagctggt
     3601 cgcgctgggc tggacggtaa ccgagttcgc ggcgggcaag tacgacatca cccggcccaa
     3661 ggcggcaggc tgcccccccc ccactctatt gtaaacaaga catttttatc ttttatattc
     3721 aatggcttat tttcctgcta attggtaata ccatgaaaaa taccatgctc agaaaaggct
     3781 taacaatatt ttgaaaaatt gcctactgag cgctgccgca cagctccata ggccgctttc
     3841 ctggctttgc ttccagatgt atgctcttct gctcctgcag ctaatggatc accgcaaaca
     3901 ggttactcgc ctggggattc cctttcgacc cgagcatccg tatgatactc atgctcgatt
     3961 attattatta tagaagcccc catgaataaa tcgctcatca ttttcggcat cgtcaacata
     4021 acctcggaca gtttctccga tggaggccgg tatctggcgc cagacgcagc cattgcgcag
     4081 gcgcgtaagc tgatggccga gggggcagat gtgatcgacc tcggtccggc atccagcaat
     4141 cccgacgccg cgcctgtttc gtccgacaca gaaatcgcgc gtatcgcgcc ggtgctggac
     4201 gcgctcaagg cagatggcat tcccgtctcg ctcgacagtt atcaacccgc gacgcaagcc
     4261 tatgccttgt cgcgtggtgt ggcctatctc aatgatattc gcggttttcc agacgctgcg
     4321 ttctatccgc aattggcgaa atcatctgcc aaactcgtcg ttatgcattc ggtgcaagac
     4381 gggcaggcag atcggcgcga ggcacccgct ggcgacatca tggatcacat tgcggcgttc
     4441 tttgacgcgc gcatcgcggc gctgacgggt gccggtatca aacgcaaccg ccttgtcctt
     4501 gatcccggca tggggttttt tctgggggct gctcccgaaa cctcgctctc ggtgctggcg
     4561 cggttcgatg aattgcggct gcgcttcgat ttgccggtgc ttctgtctgt ttcgcgcaaa
     4621 tcctttctgc gcgcgctcac aggccgtggt ccgggggatg tcggggccgc gacactcgct
     4681 gcagagcttg ccgccgccgc aggtggagct gacttcatcc gcacacacga gccgcgcccc
     4741 ttgcgcgacg ggctggcggt attggcggcg ctgaaagaaa ccgcaagaat tcgttaactg
     4801 cacattcggg atatttctct atattcgcgc ttcatcagaa aactgaagga acctccattg
     4861 aatcgaacta tatttttttt ggtgaatcgc attctgactg gttgcctgtc agaggcggag
     4921 aatctggtga ttttgttttt cgacgtggtg acgggcatgc cttcgcgaaa atcgcacctg
     4981 cttcccgccg cggtgagctc gctggagagc gtgaccgcct catttggctc aaaggtcgag
     5041 gtgtggcttg ccccgaggtc atcaactggc aggaggaaca ggagggtgca tgcttggtga
     5101 taacggcaat tccgggagta ccggcggctg atctgtctgg agcggatttg ctcaaagcgt
     5161 ggccgtcaat ggggcagcaa cttggcgctg ttcacagcct atcggttgat caatgtccgt
     5221 ttgagcgcag gctgtcgcga atgttcggac gcgccgttga tgtggtgtcc cgcaatgccg
     5281 tcaatcccga cttcttaccg gacgaggaca agagtacgcc gcagctcgat cttttggctc
     5341 gtgtcgaacg agagctaccg gtgcggctcg accaagagcg caccgatatg gttgtttgcc
     5401 atggtgatcc ctgcatgccg aacttcatgg tggaccctaa aactcttcaa tgcacgggtc
     5461 tgatcgacct tgggcggctc ggaacagcag atcgctatgc cgatttggca ctcatgattg
     5521 ctaacgccga agagaactgg gcagcgccag atgaagcaga gcgcgccttc gctgtcctat
     5581 tcaatgtatt ggggatcgaa gcccccgacc gcgaacgcct tgccttctat ctgcgattgg
     5641 accctctgac ttggggttga tgttcatgcc gcctgttttt cctgctcatt ggcacgtttc
     5701 gcaacctgtt ctcattgcgg acaccttttc cagcctcgtt tggaaagttt cattgccaga
     5761 cgggactcct gcaatcgtca agggattgaa acctatagaa gacattgctg atgaactgcg
     5821 cggggccgac tatctggtat ggcgcaatgg gaggggagca gtccggttgc tcggtcgtga
     5881 gaacaatctg atgttgctcg aatatgccgg ggagcgaatg ctctctcaca tcgttgccga
     5941 gcacggcgac taccaggcga ccgaaattgc agcggaacta atggcgaagc tgtatgccgc
     6001 atctgaggaa cccctgcctt ctgcccttct cccgatccgg gatcgctttg cagctttgtt
     6061 tcagcgggcg cgcgatgatc aaaacgcagg ttgtcaaact gactacgtcc acgcggcgat
     6121 tatagccgat caaatgatga gcaatgcctc ggaactgcgt gggctacatg gcgatctgca
     6181 tcatgaaaac atcatgttct ccagtcgcgg ctggctggtg atagatcccg tcggtctggt
     6241 cggtgaagtg ggctttggcg ccgccaatat gttctacgat ccggctgaca gagacgacct
     6301 ttgtctcgat cctagacgca ttgcacagat ggcggacgca ttctctcgtg cgctggacgt
     6361 cgatccgcgt cgcctgctcg accaggcgta cgcttatggg tgcctttccg cagcttggaa
     6421 cgcggatgga gaagaggagc aacgcgatct agctatcgcg gccgcgatca agcaggtgcg
     6481 acagacgtca tactagatat caagcgactt ctcctatccc ctgggaacac atcaatctca
     6541 ccggagaata tcgctggcca aagccttagc gtaggattcc gccccttccc gcaaacgacc
     6601 cctttctggc gcgtttgcag ggccatagac ggccgccagc ccagcggcga gggcaaccag
     6661 cccggtgagc gtcggaaagg cgctggaagc cccgtagcga cgcggagagg ggcgagacaa
     6721 gccaagggcg caggctcgat gcgcagcacg acatagccgg ttctcgcaag gacgagaatt
     6781 tccctgcggt gcccctcaag tgtcaatgaa agtttccaac gcgagccatt cgcgagagcc
     6841 ttgagtccac gctagatcta tctcatctgc gcaaggcaga acgtgaagac ggccgccctg
     6901 gacctcgccc gcgagcgcca ggcgcacgag gccggcgcgc ggacccgcgc cacggcccac
     6961 gagcggacgc cgcagcagga gcgccagaag gccgccagag aggccgagcg cggccgtgag
     7021 gcttggacgc tagggcaggg catgaaaaag cccgtagcgg gctgctacgg gcgtctgacg
     7081 cggtggaaag ggggagggga tgttgtctac atggctctgc tgtagtgagt gggttgcgct
     7141 ccggcagcgg tcctgatcaa tcgtcaccct ttctcggtcc ttcaacgttc ctgacaacga
     7201 gcctcctttt cgccaatcca tcgacaatca ccgcgagtcc ctgctcgaac gctgcgtccg
     7261 gaccggcttc gtcgaaggcg tctatcgcgg cccgcaacag cggcgagagc ggagcctgtt
     7321 caacggtgcc gccggcctgc tcctcaagca cggccccaac agtgaagtag ctgattgtca
     7381 tcagcgcatt gacggcgtcc ccggccgaaa aacccgcctc gcagaggaag cgaagctgcg
     7441 cgtcggccgt ttccatctgc ggtgcgcccg gtcgcgtgcc ggcatggatg cgcgcgccat
     7501 cgcggtaggc gagcagcgcc tgcctgaagc tgcgggcatt cccgatcaga aatgagcgcc
     7561 agtcgtcgtc ggctctcggc accgaatgcg tatgattctc cgccagcatg gcttcggcca
     7621 gtgcgtcgag cagcgcccgc ttgttcctga agtgccagta aagcgccggc tgctgaaccc
     7681 ccaaccgttc cgccagtttg cgtgtcgtca gaccgtctac gccgacctcg ttcaacaggt
     7741 ccagggcggc acggatcact gtattcggct gcaactttgt catgcttgac actttatcac
     7801 tgataaacat aatatgtcca tcaacttatc agtgataaag aatccgcgcg ttcaatcgga
     7861 ccagcggagg ctggtccgga ggccagacgt gaaacccaac agacccctga tcgtaattct
     7921 gagcactgtc gcgctcgacg ctgtcggcat cggcctgatt atgccggtgc tgccgggcct
     7981 cctgcgcgat ctggttcact cgaacgacgt cgccgcccac tatggcattc tgctggcgct
     8041 gtatgcgttg atgcaatttg cctgcgcacc tgtgctgggc gcgctgtcgg atcgtttcgg
     8101 gcggcggccg gtcttgctcg tctcgctggc cggcgctgct gtcgactacg ccatcatggc
     8161 gacggcgcct ttcctttggg ttctctatat cgggcggatc gtggccggca tcaccggggc
     8221 gactggggcg gtagccggcg cttatattgc cgatatcact gatggcgatg agcgcgcgcg
     8281 gcacttcggc ttcatgagcg cctgtttcgg gttcgggatg gtcgcgggac ctgtgctcgg
     8341 tgggctgatg ggcggtttct ccccccacgc tccgttcttc gccgcggcag ccttgaacgg
     8401 cctcaatttc ctgacgggct gtttcctttt gccggagtcg cacaaaggcg aacgccggcc
     8461 gttacgccgg gaggctctca acccgctcgc ttcgttccgg tgggcccggg gcatgaccgt
     8521 cgtcgccgcc ctgatggcgg tcttcttcat catgcaactt gtcggacagg tgccggccgc
     8581 gctttgggtc attttcggcg aggatcgctt tcactgggac gcgaccacga tcggcatttc
     8641 gcttgccgca tttggcattc tgcattcact cgcccaggca atgatcaccg gccctgtagc
     8701 cgcccggctc ggcgaaaggc gggcactcat gctcggaatg attgccgacg gcacaggcta
     8761 catcctgctt gccttcgcga cacggggatg gatggcgttc ccgatcatgg tcctgcttgc
     8821 ttcgggtggc atcggaatgc cggcgctgca agcaatgttg tccaggcagg tggatgagga
     8881 acgtcagggg cagctgcaag gctcactggc ggcgctcacc agcctgacct cgatcgtcgg
     8941 acccctcctc ttcacggcga tctatgcggc ttctataaca acgtggaacg ggtgggcatg
     9001 gattgcaggc gctgcccctc tacttgctct gcctgccggc gctgcgtcgc gggctttgga
     9061 gcggcgcagg gcaacgagcc gatcgctgat agaggtttat agcggcttcg ttcccttccg
     9121 caaccgtcag catgatctgc tccacatgct cgcggccata gtctatcacc gcccgcagca
     9181 gcataccggc gacccctcgg cctcgctgtt cgggctccac gaaaacgccg gacagatgcg
     9241 ccttgtgagc gtccttgggg ccgtcctcct gtttgaagac cgacagccca atgatctcgc
     9301 cgtcgatgta ggcgccgaat gccacggcat ctcgcaaccg ttcagcgaac gcctccatgg
     9361 gctttttctc ctcgtgctcg taaacggacc cgaacatctc tggagctttc ttcagggccg
     9421 acaatcggat ctcgccgaaa tgcctgccgt tgctagacat tgccagccag tgcccgtcac
     9481 tcccgtacta actgtcacga acccctgcaa taactgtcac gcccccctgc aataactgtc
     9541 acgaacccct gcaataactg tcacgccccc aaacctgcaa acccagcagg ggcgggggct
     9601 ggcggggtgt tggaaaaatc catccatgat tatctaagaa taatccacta ggcgcggtta
     9661 tcagcgccct tgtggggcgc tgctgccctt gcccaatatg cccggccaga ggccggatag
     9721 ctggtctatt cgctgcgcta ggctacacac cgccccaccg ctgcgcggct gggggaaagg
     9781 cgggcaaagc ccgctaaacc ccacaccaaa ccccgcagaa atacgctgga gcgcttttag
     9841 ccgctttagc ggcctttccc cctacccgaa gggtgggggc gcgtgtgcag ccccgcaggg
     9901 cctgtctcgg tcgatcattc agcccggctc atccttctgg cgtggcggca gaccgaacaa
     9961 ggcgcggtcg tggtcgcgtt caaggtacgc atccattgcc gccatgagcc gatcctccgg
    10021 ccactcgctg ctgttcacct tggccaaaat catggccccc accagcacct tgcgccttgt
    10081 ttcgttcttg cgctcttgct gctgttccct tgcccgcacc cgctgaattt cggcattgat
    10141 tcgcgctcgt tgttcttcga gcttggccag ccgatccgcc gccttgttgc tccccttaac
    10201 catcttgaca ccccattgtt aatgtgctgt ctcgtaggct atcatggagg cacagcggcg
    10261 gcaatcccga ccctactttg taggggaggg cgcacttacc ggtttctctt caagaaactg
    10321 gcctaacggc cacccttcgg gcggtgcgct ctccgagggc cattgcatgg agccgaaaag
    10381 caaaagcaac agcgaggcag catggcgatt tatcacctta cggcgaaaac cggcagcagg
    10441 tcgggcggcc aatcggccag ggccaaggcc gactacatcc agcgcgaagg caagtatgcc
    10501 cgcgacatgg atgaagtctt gcacgccgaa tccgggcaca tgccggagtt cgtcgagcgg
    10561 cccgccgact actgggatgc tgccgacctg tatgaacgcg ccaatgggcg gctgttcaag
    10621 gaggtcgaat ttgccctgcc ggtcgagctg accctcgacc agcagaaggc gctggcgtcc
    10681 gagttcgccc agcacctgac tggtgccgag cgcctgccgt atacgctggc catccatgcc
    10741 ggtggcggcg agaacccgca ctgccacctg atgatctccg agcggatcaa tgacggcatc
    10801 gagcggcccg ccgctcagtg gttcaagcgg tacaacggca agaccccgga gaagggcggg
    10861 gcacagaaga ccgaagcgct caagcccaag gcatggcttg agcagacccg cgaggcatgg
    10921 gccgaccatg ccaaccgggc attagagcgg gctggccacg acgcccgcat tgaccacaga
    10981 acacttgagg cgcagggcat cgagcgcctg cccggtgttc acctggggcc gaacgtggtg
    11041 gagat
//
