LOCUS       CP061249               14260 bp    DNA     circular BCT 13-JAN-2022
DEFINITION  Escherichia coli strain STEC401 plasmid pSTEC401_2, complete
            sequence.
ACCESSION   CP061249
VERSION     CP061249.1
DBLINK      BioProject: PRJNA655129
            BioSample: SAMN15748003
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14260)
  AUTHORS   Pan,Y., Xiong,Y. and Yang,X.
  TITLE     Genomic characteristics of Shiga toxin-producing Escherichia coli
            (STEC)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14260)
  AUTHORS   Pan,Y., Xiong,Y. and Yang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2020) National Institute for Communicable Disease
            Control and Prevention, Chinese Center for Disease Control and
            Prevention, No. 155, Changbai Rd, Beijing 102200, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. v. 4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 09/02/2020 12:04:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.12
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,149
            CDSs (total)                      :: 5,027
            Genes (coding)                    :: 4,245
            CDSs (with protein)               :: 4,245
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 782
            CDSs (without protein)            :: 782
            Pseudo Genes (ambiguous residues) :: 0 of 782
            Pseudo Genes (frameshifted)       :: 691 of 782
            Pseudo Genes (incomplete)         :: 88 of 782
            Pseudo Genes (internal stop)      :: 64 of 782
            Pseudo Genes (multiple problems)  :: 56 of 782
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..14260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="STEC401"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSTEC401_2"
                     /geo_loc_name="China: Henan"
                     /collection_date="2013"
     gene            complement(join(13976..14260,1..40))
                     /locus_tag="H6S16_25635"
                     /pseudo=""
     CDS             complement(join(13976..14260,1..40))
                     /locus_tag="H6S16_25635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            108..299
                     /locus_tag="H6S16_25640"
     CDS             108..299
                     /locus_tag="H6S16_25640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UIV22968.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDVDDQADICEALHDHA
                     DELYRSCLAHFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(450..719)
                     /locus_tag="H6S16_25645"
     CDS             complement(450..719)
                     /locus_tag="H6S16_25645"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UIV22969.1"
                     /translation="MEKRQRRGFFCPLPFCSHVLFCLIPDSVDNRIAVFEQADSARRSR
                     TTERSESVSEEAEKCPDEHFLLTHLCGISHPAWCTFHTILTDAT"
                     /name="hypothetical protein"
     gene            complement(1295..1450)
                     /gene="csl"
                     /locus_tag="H6S16_25650"
     CDS             complement(1295..1450)
                     /gene="csl"
                     /locus_tag="H6S16_25650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071703583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 system lysis protein"
                     /protein_id="UIV22970.1"
                     /translation="MKKIIICAISLGIMLLAACQVNNVRDTGGGAVSPSSTVTGVSMGS
                     GGSGSL"
                     /name="csl"
     gene            1480..2018
                     /gene="csi"
                     /locus_tag="H6S16_25655"
                     /pseudo=""
     CDS             1480..2018
                     /gene="csi"
                     /locus_tag="H6S16_25655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355619.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 immunity protein"
                     /name="csi"
     gene            complement(2046..3545)
                     /gene="csa"
                     /locus_tag="H6S16_25660"
     CDS             complement(2046..3545)
                     /gene="csa"
                     /locus_tag="H6S16_25660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin S4"
                     /protein_id="UIV22971.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGSG
                     NGGREHGSQTGWGWSKTNNPDVPPYVDDNGQVRITITNGLVKTPVYGVPGAGGNSDVQG
                     GYIPENPNDEVARKWDKNNLPREIDVSIDGFKYRVTLNDNGRAIGILRTGVRPYVGSEK
                     AKAGIMEKINHKTPEEIYEALGFNKDESQRQEKAKQQAEDAWDRLPPNVRKFDVDVEQF
                     HYLVVLDDYGNVLSVTRTAVRPYVGSEKAKAGIMDKVDHKTPEEIYEALGFNNEEPQRQ
                     DQAKKAAYDVFYSFSMNRDRIQSDVLNKAAEVISDIGNKVGDYLGDDYKSLAREIADDV
                     KNFQGKTIRSYDDAMASLNKVLSNPGFKFNRADSDALANVWRSIDAQDMANKLGNISKA
                     FKFADVVMKVEKVREKSIEGYETGNWGPLMLEVESWVLSGIASAVALGVFSATLGAYAL
                     SLGAPAIAVGIVGILLAAVVGALIDDKFADALNKEIIKPAH"
                     /name="csa"
     gene            3784..4074
                     /locus_tag="H6S16_25665"
     CDS             3784..4074
                     /locus_tag="H6S16_25665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UIV22972.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPST"
                     /name="hypothetical protein"
     gene            4465..4887
                     /gene="exc2"
                     /locus_tag="H6S16_25670"
     CDS             4465..4887
                     /gene="exc2"
                     /locus_tag="H6S16_25670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="UIV22973.1"
                     /translation="MRSAATRLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRAKEFHSEKFFHSLQSTTT
                     FAGRKYTNSDMPSLKKMKLMADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            complement(5259..5453)
                     /locus_tag="H6S16_25675"
     CDS             complement(5259..5453)
                     /locus_tag="H6S16_25675"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="UIV22974.1"
                     /translation="MRRLESAGAEISRAGGPLESVIYHEQQIRDEQERQARLERERARS
                     WHEEQEREARVWRGPSMGM"
                     /name="mobilization protein"
     gene            complement(5369..5602)
                     /gene="mbeD"
                     /locus_tag="H6S16_25680"
     CDS             complement(5369..5602)
                     /gene="mbeD"
                     /locus_tag="H6S16_25680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UIV22978.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="mbeD"
     gene            complement(5608..6168)
                     /locus_tag="H6S16_25685"
     CDS             complement(5608..6168)
                     /locus_tag="H6S16_25685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="UIV22975.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLIKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(6140..6637)
                     /locus_tag="H6S16_25690"
                     /pseudo=""
     CDS             complement(6140..6637)
                     /locus_tag="H6S16_25690"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(6637..6855)
                     /locus_tag="H6S16_25695"
     CDS             complement(6637..6855)
                     /locus_tag="H6S16_25695"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UIV22976.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGVRVLRGAPEEVRELID
                     ATPFAKKYTSGVLSFAEQTLPPESVKG"
                     /name="hypothetical protein"
     gene            complement(6845..7168)
                     /locus_tag="H6S16_25700"
     CDS             complement(6845..7168)
                     /locus_tag="H6S16_25700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UIV22977.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            7236..7427
                     /locus_tag="H6S16_25705"
     CDS             7236..7427
                     /locus_tag="H6S16_25705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UIV22964.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDVDDQADICEALHDHA
                     DELYRSCLAHFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(8425..8580)
                     /gene="csl"
                     /locus_tag="H6S16_25710"
     CDS             complement(8425..8580)
                     /gene="csl"
                     /locus_tag="H6S16_25710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071703583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 system lysis protein"
                     /protein_id="UIV22965.1"
                     /translation="MKKIIICAISLGIMLLAACQVNNVRDTGGGAVSPSSTVTGVSMGS
                     GGSGSL"
                     /name="csl"
     gene            8610..9149
                     /gene="csi"
                     /locus_tag="H6S16_25715"
     CDS             8610..9149
                     /gene="csi"
                     /locus_tag="H6S16_25715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 immunity protein"
                     /protein_id="UIV22966.1"
                     /translation="MMIDEHSIDIDNRKANNLLYLFMVIGVIPLLCILAVYYTNPDNLF
                     LHTIATSTENIPSITSAYNPLMTKVMDIYCKTAPSLALILFILTFKTRKLIKKTNRNAV
                     LRSCLLSPFVCAVFLYLLCFRNLELTTAGRPARLMTNNDATLLLFYIGLYLIIFFISYF
                     TLFTPVTTFKLLKERQ"
                     /name="csi"
     gene            complement(9177..10674)
                     /gene="csa"
                     /locus_tag="H6S16_25720"
                     /pseudo=""
     CDS             complement(9177..10674)
                     /gene="csa"
                     /locus_tag="H6S16_25720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355618.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin S4"
                     /name="csa"
     gene            10913..11161
                     /locus_tag="H6S16_25725"
                     /pseudo=""
     CDS             10913..11161
                     /locus_tag="H6S16_25725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221218.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            11594..12016
                     /gene="exc2"
                     /locus_tag="H6S16_25730"
     CDS             11594..12016
                     /gene="exc2"
                     /locus_tag="H6S16_25730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="UIV22967.1"
                     /translation="MRSAATRLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRAKEFHSEKFFHSLQSTTT
                     FAGRKYTNSDMPSLKKMKLMADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            complement(12498..12730)
                     /gene="mbeD"
                     /locus_tag="H6S16_25735"
                     /pseudo=""
     CDS             complement(12498..12730)
                     /gene="mbeD"
                     /locus_tag="H6S16_25735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /name="mbeD"
     gene            complement(12737..13298)
                     /locus_tag="H6S16_25740"
                     /pseudo=""
     CDS             complement(12737..13298)
                     /locus_tag="H6S16_25740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /name="MbeB family mobilization protein"
     gene            complement(13267..14013)
                     /locus_tag="H6S16_25745"
                     /pseudo=""
     CDS             complement(13267..14013)
                     /locus_tag="H6S16_25745"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          complement(386..471)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.727"
                     /coverage="99.0"
                     /evalue="1.9199999999999999e-34"
     Typing          complement(7514..7600)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.864"
                     /coverage="99.0"
                     /evalue="1.15e-36"
     Typing          495..1038
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.613"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          7624..8168
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.796"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(100..302)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="86.765"
                     /coverage="100.0"
                     /evalue="1.43e-10"
     Typing          complement(5610..6712)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.109"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6849..7276)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="88.811"
                     /coverage="100.0"
                     /evalue="9.87e-78"
     Typing          complement(13049..13986)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.361"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gccgcgcatg ttcctcatcc gtgacccgta tcgtgagcat cctttcgcgt ttcaccggta
       61 tcattaaaaa cctccgacag actccccaca catggagaaa cagaactgtg actaaacagg
      121 aaaaaaccgc ccttaacatg gcccgcttca tcagaagcca gacgctgacc ctgctggaaa
      181 aactgaatga actggacgtc gacgaccagg ctgacatctg cgaagcgctt cacgaccacg
      241 ctgacgagct ttaccgcagc tgcctcgcac acttcgggga tgacggtgaa aacctctgac
      301 acatgcagct cccggagacg gtcacagctg gcctgtgagc ggatgccggg agcagacaag
      361 cccgtcaggg cgcgtcagcg ggtgttagcg gtgtcggggc gcagccatga cccagtcacg
      421 tagcgatagc ggagtgtata ctggcttaat catgtggcat cagtgaggat tgtatgaaaa
      481 gtgcaccatg ccgggtgtga aatgccgcac agatgcgtaa ggagaaaatg ctcgtccggg
      541 cacttttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcgact gcggcgagcg
      601 gagtctgctt gctcaaaaac ggcgatacgg ttatccacag aatcagggat aaggcagaaa
      661 agaacatgtg agcaaaaagg caaagggcaa aaaaagccgc gtcgctggcg tttttccata
      721 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
      781 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
      841 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
      901 tttctcatag ctcacgctgt tggtatctca gttcggtgta ggtcgttcgc tccaagctgg
      961 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     1021 tcgagtccac cccggtaaga cacgacaaag cgccactggc agcagccatt ggtaactgaa
     1081 aagtggattt agatacgcag aactcttgaa gttgaagcct tatagcggct acactgaaag
     1141 gacagcattt ggtatctgtg ctccacttaa gccagctacc cggttaagca gtccccaact
     1201 gacttaacct tcgactaaac cgcctcccca ggcggttttt tcgtttacag ggcaagagac
     1261 tacgacgatc tgtaaaggat ctcaagaaga tcctttacag gctaccacta ccaccacttc
     1321 ccatactcac tccggtaaca gtggaagatg gtgaaacagc cccacctcca gtatctctga
     1381 cattgttgac ttggcatgct gcaagcagca ttattcctag tgaaatggca caaataataa
     1441 tttttttcat atataccttt attataatcc tataggtcaa tgatgattga tgaacattca
     1501 atagatatag acaatagaaa agccaataac ttattgtatt tatttatggt aatcggagtt
     1561 atacctttac tctgtatact agccgtttac tatacaaatc cagacaatct gttcttgcac
     1621 acaatagcta ccagtactga aaacatccca tcaataacct ccgcctacaa cccattaatg
     1681 acaaaggtta tggatattta ttgtaaaaca gcgccttccc ttgctttgat attatttatc
     1741 ctgacattta aaacgagaaa gctaataaaa aaacaaaccg aaatgctgta cttagaagtt
     1801 gcttattaag tccatttgtc tgtgctgttt ttttgtattt actctgtttc cgaaaccttg
     1861 agctaacgac agcaggaagg ccagccaggc taatgaccaa caatgatgca acactattgc
     1921 tattttatat tggcctgtac ttaatcattt ttttcataag ctatttcacg ctattcacac
     1981 cagttaccac gtttaaatta ttaaaagaaa ggcagtaacc ctgccttcct tttacccggt
     2041 atttattaat gcgcgggttt aatgatttct ttatttagtg catctgcaaa cttgtcatca
     2101 attaatgcac ctacaactgc tgcgagtaga atacctacga taccgacagc aatagcagga
     2161 gccccaagag ataaggcata tgcccctaat gtggctgaaa aaacacccag ggccacagca
     2221 gaagcgatgc cactgagcac ccaggactcc acctccagca tcagaggtcc ccagttcccg
     2281 gtttcatagc cctcgatgct tttctcccgg accttctcaa ccttcatcac cacatcggca
     2341 aatttgaaag ccttgctgat attacccagt ttatttgcca tatcctgagc atcaatactt
     2401 cgccagacgt tcgccagtgc atcactgtcg gccctgttaa atttaaaccc cggattagac
     2461 aaaactttat ttaatgacgc cattgcatca tcgtagctac ggatagtttt cccctggaaa
     2521 tttttgacat catcagcaat ttctcttgct aacgacttat aatcatctcc aagataatca
     2581 ccgaccttat tacctatatc agagataact tcagcggctt tattaagtac atcactctga
     2641 atccggtcac ggttcatact gaaagaataa aacacatcat aagcagcttt tttagcttga
     2701 tcctgtcgtt gaggctcttc attattaaat cctaatgcct catatatctc ttccggggtc
     2761 ttatggtcga ctttatccat aataccagcc ttagcttttt cactccctac ataaggacga
     2821 acagctgttc tagttacaga taaaacatta ccataatcat caagcaccac taaatagtgg
     2881 aactgttcaa catcaacatc aaattttctc acattaggag gcaaacgatc ccacgcatct
     2941 tcagcctgtt gttttgcttt ttcctgccgc tgagattcat ctttattaaa acctaacgcc
     3001 tcatatatct cctcaggagt tttatgattg attttttcca taatacccgc cttagctttt
     3061 tcactcccta cgtaaggacg gacacctgtt cgaagaatac cgatagctct accattatca
     3121 ttaagcgtta cacgatattt aaaaccatcg atactcacat caatctctcg cggtaagttg
     3181 tttttatccc acttacgagc aacttcatca ttaggatttt cagggatata gcccccctga
     3241 acatcactat ttccgccagc cccaggaact ccatatacag gcgtttttac cagaccattt
     3301 gtgatggtaa tgcgcacctg cccattatca tcaacataag gcggcacatc cggattattg
     3361 gttttactcc atccccaccc cgtctgactt ccatgttcac gacctccatt accagagcct
     3421 gaaccaccac cccagtgaac cccactatta ctattattat ttccaccttg ctgattcaga
     3481 ttcgcccctg ttccccccat agactcacca gcagttgggc catatccact tagttcttta
     3541 gccataaatt cctctttgat aattaaaaca ataaattaaa aacagtatac tgtacatata
     3601 accactggtt ttatgtacag caaaacctac tactcaccat tgtccatgtc aagagcatgg
     3661 attttcattt ttgctataag gatcacgcta tggggaggca ggcattgaga acgtcgaaac
     3721 agaacaccgg agcaaatcag gatgagatat aaaactgttg gatcatgaaa aacggagaac
     3781 gatgtgagca aatcaccccg ccataaactg aacaaaacag acaaacgact tctcgacacc
     3841 cttgttgctg ccggatatga gcatgacaaa gcccgtgacc tcatccagaa acaggtttac
     3901 acgctgacac tggctgatca gcgtcatgtg gtcagtgaaa tcagtaatgg tgtgaatccc
     3961 acccaggctt actcggcggt ataccaggca agacgcattc gcctcgcccg taaatatctg
     4021 aacggtaaaa aggttatgga agaaaccggg gaaaatacgc ccccatcaac gtaaggattt
     4081 ttttgccgct ccagagactc cagtttttta cgcaaatcct ctcttttttg ggcatctctg
     4141 gtgccagcca gctctgctct caactcatcg atctgaagtt gtatcttcag tctgttactg
     4201 aacattttct gtctggcatt aacatccgca acaatgccgt tttttgtctt ctcggccttt
     4261 tgttgaaaaa cattgctgtc cgcatgactg gcaaccgaag cagaaagaac actaaaaagc
     4321 aggactggca cacatttttt cacgggatta ttcctgactc attgaccatc aaatcacatt
     4381 gggagtaaac cgacgtatga taagagatac tcttcggaga tataactccc tgagtatcaa
     4441 gattaaaaac gcaaggagat gtttatgaga tctgccgcta ccaggctgct tctgatacct
     4501 ctgataacag caacaatagc tcttacagga tgcaccccaa agaccagcct ggaacgacat
     4561 acccgacatt atgtttatgc ttcagatgat ggatttgatc ctaacttcta tacccagaaa
     4621 gcagacacca tacgtatgat gctcccgttc tttcagcagt tccgggatat ggggatgaaa
     4681 gacaaagcag ccggagtatc agcagaaacg gcacagcaac gtgcaaaaga attccactca
     4741 gaaaaatttt ttcactcact ccagagcaca acaacctttg ctggcagaaa atacacaaac
     4801 agcgatatgc cttcgctgaa aaaaatgaaa ctaatggcag acaccatttc tgcggtttat
     4861 ctcgatggat acgagggcag acagtaaggg atttaccata atcccttaat tgtacgcacc
     4921 gctgaaatgc gttcagcgcg atcacggctg ctgacaggta aaatggcaac aaaccacccg
     4981 aaaagctgcc gcgatcgcac ctgataaatt ttaaccgtat gcatagctat tcagccatgt
     5041 gaataacgct ggttttgcct gcgtaaacct catgacactg tttttttcca tcttttcagt
     5101 tgatgacata cgcagacatc gcgggatgag gctgaggaat gagcgcgatc tggcaaagag
     5161 gcaaaacaca gcaacaaaaa cgacactcca gaatcgcgcc cggatgcgtt tttaacgcgt
     5221 tccggtacca tctggcaacc tcccggaaca actcaccgtc acatccccat tgacgggcca
     5281 cgccataccc gtgcttcccg ttcctgctct tcatgccagg accgcgcacg ctcccgttcc
     5341 aggcgtgcct gcctttcctg ttcatccctt atctgctgtt cgtgataaat aaccgactca
     5401 agcggtccac ctgcccggct aatctctgca cctgctgact caagtcgtcg cactgttccc
     5461 tcagttgccc gttctcctgt cgtgtcagct cgaacatatg ctgcaaatcc gtgaaggcgc
     5521 tctcccagtc tttcagccgc tgcgtatagt cctgctgcaa ttgctctaag gcgttcagta
     5581 agtgcatttc cagctctgtc atactcacta ctccctgacc agtcttactg cgttcttctt
     5641 ctccaccgtc cagttgtttt cccccttcac cccggacggc agcaccagaa atttcccgtt
     5701 cctgccctcg tgatacgtca caccccatgt tttttcccgg agtttcgcca gcgtctcttc
     5761 ctggtccctg atagccagga tgttcgccgc aatccggctt tcctgccact gaatcagccc
     5821 ccatgacgcc agaaaaatcc cgcccgtgac gcaaagcgcc gtcagcgacg ggtacagtat
     5881 ccgccctttg atcagcttcc agagcagctc ttcctgccgc cgggccagtt cgttctctgt
     5941 ggcgctgaac tgcgcgttca cggcactgtt cagcgtctcc agttgttctt tcaccgctgc
     6001 tgtgtgtgcg ctgatagcgt ctctgatttt ctgcccgttt aagttcagtt ccctgtctac
     6061 agacgcttcg agcttcctga actcgctgtt cagcatgttc tctgtagaga cggcacgctc
     6121 tttcagtttc ttctcgaagt ctgtccccat ttgtaaaaga ttgctcatac agcgcccctt
     6181 tcagcctgag attacgcccg ccctccgggt cggcgatact gatactgctc ctggttgtcc
     6241 tcacaacctc aaaacctgcc gctgtaagcg cctcagtgac atcctgacgc gtttttagcg
     6301 ctccggcatg gtaaagagcc tccagtcccc tcgtaatcgc ttctgcggcc tcctgtttcg
     6361 ctttcggcag attattcggg gtgacaagtg tccgcctgtt ctccggtgcg ttcgggtcgt
     6421 gcagcccgta atggtgattc accagtgtct gccaggcatt gattcgcgga cggtccgccc
     6481 ggtcgtaata gggctggagc cgttttccgc tcgccagctc catattcggg atgacaaaat
     6541 tcagctcaag acgccccttg tcctggtgct ccacccacag gatgctgtac tgattttttt
     6601 caagaccggg catcagtacc cgctcaaagc tctccatcac cctttcacgc tctccggtgg
     6661 cagggtctgc tctgcaaaag acagaacccc cgaggtgtat tttttcgcaa acggcgtggc
     6721 atcgatgagt tcccgtacct cttcgggagc accccgcaga actctcaccc cttcccggtt
     6781 acgctcccgg cccagcaggt aatcaaccgg accactgcca ccgccttttc ccctggcatg
     6841 aaacttaact atcatcccgt tctccctgtt tacggacctc atccctaagc tcactcagtt
     6901 cacgtccgat ggccatcagt gcagccacca catgaacccg gtcatgcccc gaccactgtc
     6961 cgctgtttat cttccgggct atctgattca ggttattgcc gaccgaagcg aactggcgca
     7021 acagcggcgg tgccagtgtc ggaagacctg acgttttcga tggcggtgcc cccaggcaga
     7081 ccttacgcat ccatgacgca agttgttttc cctcacaacg tgccagcagc cgcgcatgtt
     7141 cctcatccgt gacccgtatc gtgagcatcc tttcgcgttt caccggtatc attaaaaacc
     7201 tccgacagac tccccacaca tggagaaaca gaactgtgac taaacaggaa aaaaccgccc
     7261 ttaacatggc ccgcttcatc agaagccaga cgctgaccct gctggaaaaa ctgaatgaac
     7321 tggacgtcga cgaccaggct gacatctgcg aagcgcttca cgaccacgct gacgagcttt
     7381 accgcagctg cctcgcacac ttcggggatg acggtgaaaa cctctgacac atgcagctcc
     7441 cggagacggt cacagctggc ctgtgagcgg atgccgggag cagacaagcc cgtcagggcg
     7501 cgtcagcggg tgttagcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg
     7561 gagtgtatac tggcttaatc atgtggcatc agtgaggatt gtatgaaaag tgcaccatgc
     7621 cgggtgtgaa atgccgcaca gatgcgtaag gagaaaatgc tcgtccgggc acttttccgc
     7681 ttcctcgctc actgactcgc tgcgctcggt cgttcgactg cggcgagcgg agtctgcttg
     7741 ctcaaaaacg gcgatacggt tatccacaga atcaggggat aaggcagaaa agaacatgtg
     7801 agcaaaaagg caaagggcaa aaaaagccgc gtcgctggcg tttttccata ggctccgccc
     7861 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     7921 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     7981 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     8041 ctcacgctgt tggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     8101 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc tcgagtccac
     8161 cccggtaaga cacgacaaag cgccactggc agcagccatt ggtaactgaa aagtggattt
     8221 agatacgcag aactcttgaa gttgaagcct tatagcggct acactgaaag gacagcattt
     8281 ggtatctgtg ctccacttaa gccagctacc cggttaagca gtccccaact gacttaacct
     8341 tcgactaaac cgcctcccca ggcggttttt tcgtttacag ggcaagagac tacgacgatc
     8401 tgtaaaggat ctcaagaaga tcctttacag gctaccacta ccaccacttc ccatactcac
     8461 tccggtaaca gtggaagatg gtgaaacagc cccacctcca gtatctctga cattgttgac
     8521 ttggcatgct gcaagcagca ttattcctag tgaaatggca caaataataa tttttttcat
     8581 atataccttt attataatcc tataggtcaa tgatgattga tgaacattca atagatatag
     8641 acaatagaaa agccaataac ttattgtatt tatttatggt aatcggagtt atacctttac
     8701 tctgtatact agccgtttac tatacaaatc cagacaatct gttcttgcac acaatagcta
     8761 ccagtactga aaacatccca tcaataacct ccgcctacaa cccattaatg acaaaggtta
     8821 tggatattta ttgtaaaaca gcgccttccc ttgctttgat attatttatc ctgacattta
     8881 aaacgagaaa gctaataaaa aaaacaaacc gaaatgctgt acttagaagt tgcttattaa
     8941 gtccatttgt ctgtgctgtt tttttgtatt tactctgttt ccgaaacctt gagctaacga
     9001 cagcaggaag gccagccagg ctaatgacca acaatgatgc aacactattg ctattttata
     9061 ttggcctgta cttaatcatt tttttcataa gctatttcac gctattcaca ccagttacca
     9121 cgtttaaatt attaaaagaa aggcagtaac cctgccttcc ttttacccgg tatttattaa
     9181 tgcgcgggtt taatgatttc tttatttagt gcatctgcaa acttgtcatc aattaatgca
     9241 cctacaactg ctgcgagtag aatacctacg ataccgacag caatagcagg agccccaaga
     9301 gataaggcat atgcccctaa tgtggctgaa aaaacaccca gggccacagc agaagcgatg
     9361 ccactgagca cccaggactc cacctccagc atcagaggtc cccagttccc ggtttcatag
     9421 ccctcgatgc ttttctcccg gaccttctca accttcatca ccacatcggc aaatttgaaa
     9481 gccttgctga tattacccag tttatttgcc atatcctgag catcaatact tcgccagacg
     9541 ttcgccagtg catcactgtc ggccctgtta aatttaaacc ccggattaga caaaacttta
     9601 tttaatgacg ccattgcatc atcgtagcta cggatagttt tcccctggaa atttttgaca
     9661 tcatcagcaa tttctcttgc taacgactta taatcatctc caagataatc accgacctta
     9721 ttacctatat cagagataac ttcagcggct ttattaagta catcactctg aatccggtca
     9781 cggttcatac tgaaagaata aaacacatca taagcagctt ttttagcttg atcctgtcgt
     9841 tgaggctctt cattattaaa tcctaatgcc tcatatatct cttccggggt cttatggtcg
     9901 actttatcca taataccagc cttagctttt tcactcccta cataaggacg aacagctgtt
     9961 ctagttacag ataaaacatt accataatca tcaagcacca ctaaatagtg gaactgttca
    10021 acatcaacat caaattttct cacattagga ggcaaacgat cccacgcatc ttcagcctgt
    10081 tgttttgctt tttcctgccg ctgagattca tctttattaa acctaacgcc tcatatatct
    10141 cctcaggagt tttatgattg attttttcca taatacccgc cttagctttt tcactcccta
    10201 cgtaaggacg gacacctgtt cgaagaatac cgatagctct accattatca ttaagcgtta
    10261 cacgatattt aaaaccatcg atactcacat caatctctcg cggtaagttg tttttatccc
    10321 acttacgagc aacttcatca ttaggatttt cagggatata gccccctgaa catcactatt
    10381 tccgccagcc ccaggaactc catatacagg cgtttttacc agaccatttg tgatggtaat
    10441 gcgcacctgc ccattatcat caacataagg cggcacatcc ggattattgg ttttactcca
    10501 tccccacccc gtctgacttc catgttcacg acctccatta ccagagcctg aaccaccacc
    10561 ccagtgaacc ccactattac tattattatt tccaccttgc tgattcagat tcgcccctgt
    10621 tccccccata gactcaccag cagttgggcc atatccactt agttctttag ccataaattc
    10681 ctctttgata attaaaacaa taaattaaaa acagtatact gtacatataa ccactggttt
    10741 tatgtacagc aaaacctact actcaccatt gtccatgtca agagcatgga ttttcatttt
    10801 tgctataagg atcacgctat ggggaggcag gcattgagaa cgtcgaaaca gaacaccgga
    10861 gcaaatcagg atgagatata aaactgttgg atcatgaaaa acggagaacg atgtgagcaa
    10921 atcaccccgc cataaactga acaaaacaga caaacgactt ctcgacaccc ttgttgctgc
    10981 cggatatgag catgacaaag cccgtgacct catccagaaa caggtttaca cgctgacact
    11041 ggctgatcag cgtcatgtgg tcagtgaaat cagtaatggt gtgaatccca cccaggctta
    11101 ctcggcggta taccaggcaa gacgcattcg cctcgcccgt aaatatctga acggtaaaaa
    11161 ggttatggaa gaaaccggga aaatacgccc ccatcaacgt aaggatttct tttgccgctc
    11221 cagagactcc agttttttac gcaaatcctc tcttttttgg gcatctctgg tgccagccag
    11281 ctctgctctc aactcatcga tctgaagttg tatcttcagt ctgttactga acattttctg
    11341 tctggcatta acatccgcaa caatgccgtt ttttgtcttc tcggcctttt gttgaaaaac
    11401 attgctgtcc gcatgactgg caaccgaagc agaaagaaca ctaaaaagca ggactggcac
    11461 acattttttc acgggattat tcctgactca ttgaccatca aatcacattg ggagtaaacc
    11521 gacgtatgat aagagatact cttcggagat ataactccct gagtatcaag attaaaaacg
    11581 caaggagatg tttatgagat ctgccgctac caggctgctt ctgatacctc tgataacagc
    11641 aacaatagct cttacaggat gcaccccaaa gaccagcctg gaacgacata cccgacatta
    11701 tgtttatgct tcagatgatg gatttgatcc taacttctat acccagaaag cagacaccat
    11761 acgtatgatg ctcccgttct ttcagcagtt ccgggatatg gggatgaaag acaaagcagc
    11821 cggagtatca gcagaaacgg cacagcaacg tgcaaaagaa ttccactcag aaaaattttt
    11881 tcactcactc cagagcacaa caacctttgc tggcagaaaa tacacaaaca gcgatatgcc
    11941 ttcgctgaaa aaaatgaaac taatggcaga caccatttct gcggtttatc tcgatggata
    12001 cgagggcaga cagtaaggga tttaccataa tcccttaatt gtacgcaccg ctgaaatgcg
    12061 ttcagcgcga tcacggctgc tgacaggtaa aatggcaaca aaccacccga aaagctgccg
    12121 cgatcgcacc tgataaattt taaccgtatg catagctatt cagccatgtg aataacgctg
    12181 gttttgcctg cgtaaacctc atgacactgt ttttttccat cttttcagtt gatgacatac
    12241 gcagacatcg cgggatgagg ctgaggaatg agcgcgatct ggcaaagagg caaaacacag
    12301 caacaaaaac gacactccag aatcgcgccc ggatgcgttt ttaacgcgtt ccggtaccat
    12361 ctggcaacct cccggaacaa ctcaccgtca catccccatt gacgggccac gccatacccg
    12421 tgcttcccgt tcctgctctt catgccagga ccgcgcacgc tcccgttcca ggcgtgcctg
    12481 cctttcctgt tcatccctta tctgctgttc gtgataaata accgactcaa gcggtccacc
    12541 tgcccggcta atctctgcac ctgctgactc aagtcgtcgc actgttccct cagttgcccg
    12601 ttctcctgtc gtgtcagctc gaacatatgc tgcaaatccg tgaaggcgct ctcccagtct
    12661 ttcagccgct gcgtatagtc ctgctgcatt gctctaaggc gttcagtaag tgcatttcca
    12721 gctctgtcat actcacttac tccctgacca gtcttactgc gttcttcttc tccaccgtcc
    12781 agttgttttc ccccttcacc ccggacggca gcaccagaaa tttcccgttc ctgccctcgt
    12841 gatacgtcac accccatgtt ttttcccgga gtttcgccag cgtctcttcc tggtccctga
    12901 tagccaggat gttcgccgca atccggcttt cctgccactg aatcagcccc catgacgcca
    12961 gaaaaatccc gcccgtgacg caaagcgccg tcagcgacgg gtacagtatc cgccctttga
    13021 tcagcttcca gagcagctct tcctgcccgc cgggccagtt cgttctctgt ggcgctgaac
    13081 tgcgcgttca cggcactgtt cagcgtctcc agttgttctt tcaccgctgc tgtgtgtgcg
    13141 ctgatagcgt ctctgatttt ctgcccgttt aagttcagtt ccctgtctac agacgcttcg
    13201 agcttcctga actcgctgtt cagcatgttc tctgtagaga cggcacgctc tttcagtttc
    13261 ttctcgaagt ctgtccccat ttgtaaaaga ttgctcatac agcgcccctt tcagcctgag
    13321 attacgcccg ccctccgggt cggcgatact gatactgctc ctggttgtcc tcacaacctc
    13381 aaaacctgcc gctgtaagcg cctcagtgac atcctgacgc gtttttagcg ctccggcatg
    13441 gtaaagagcc tccagtcccc tcgtaatcgc ttctgcggcc tcctgtttcg ctttcggcag
    13501 attattcggg gtgacaagtg tccgcctgtt ctccggtgcg ttcgggtcgt gcagcccgta
    13561 atggtgattc accagtgtct gccaggcatt gattcgcgga cggtccgccc ggtcgtaata
    13621 gggctggagc cgttttccgc tcgccagctc catattcggg atgacaaaat tcagctcaag
    13681 acgccccttg tcctggtgct ccacccacag gatgctgtac tgattttttt caagaccggg
    13741 catcagtacc cgctcaaagc tctccatcac cctttcacgc tctcccggtg gcagggtctg
    13801 ctctgcaaaa gacagaaccc ccgaggtgta ttttttcgca aacggcgtgg catcgatgag
    13861 ttcccgtacc tcttcgggag caccccgcag aactctcacc ccttcccggt tacgctcccg
    13921 gcccagcagg taatcaaccg gaccactgcc accgcctttt cccctggcat gaaacttaac
    13981 tatcatcccg ttctccctgt ttacggacct catccctaag ctcactcagt tcacgtccga
    14041 tggccatcag tgcagccacc acatgaaccc ggtcatgccc cgaccactgt ccgctgttta
    14101 tcttccgggc tatctgattc aggttattgc cgaccgaagc gaactggcgc aacagcggcg
    14161 gtgccagtgt cggaagacct gacgttttcg atggcggtgc ccccaggcag accttacgca
    14221 tccatgacgc aagttgtttt tccctcacaa cgtgccagca
//
