LOCUS       CP061228                7138 bp    DNA     circular BCT 13-JAN-2022
DEFINITION  Escherichia coli strain STEC398 plasmid pSTEC398_2, complete
            sequence.
ACCESSION   CP061228
VERSION     CP061228.1
DBLINK      BioProject: PRJNA655129
            BioSample: SAMN15748001
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7138)
  AUTHORS   Pan,Y., Xiong,Y. and Yang,X.
  TITLE     Genomic characteristics of Shiga toxin-producing Escherichia coli
            (STEC)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7138)
  AUTHORS   Pan,Y., Xiong,Y. and Yang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2020) National Institute for Communicable Disease
            Control and Prevention, Chinese Center for Disease Control and
            Prevention, No. 155, Changbai Rd, Beijing 102200, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. v. 4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 09/02/2020 11:48:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.12
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,108
            CDSs (total)                      :: 4,985
            Genes (coding)                    :: 4,846
            CDSs (with protein)               :: 4,846
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 58 of 139
            Pseudo Genes (incomplete)         :: 67 of 139
            Pseudo Genes (internal stop)      :: 41 of 139
            Pseudo Genes (multiple problems)  :: 23 of 139
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="STEC398"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSTEC398_2"
                     /geo_loc_name="China: Henan"
                     /collection_date="2013"
     gene            complement(join(7075..7138,1..227))
                     /locus_tag="H6S14_25490"
     CDS             complement(join(7075..7138,1..227))
                     /locus_tag="H6S14_25490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UIV31746.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPST"
                     /name="hypothetical protein"
     gene            466..1965
                     /gene="csa"
                     /locus_tag="H6S14_25495"
     CDS             466..1965
                     /gene="csa"
                     /locus_tag="H6S14_25495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin S4"
                     /protein_id="UIV31747.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGSG
                     NGGREHGSQTGWGWSKTNNPDVPPYVDDNGQVRITITNGLVKTPVYGVPGAGGNSDVQG
                     GYIPENPNDEVARKWDKNNLPREIDVSIDGFKYRVTLNDNGRAIGILRTGVRPYVGSEK
                     AKAGIMEKINHKTPEEIYEALGFNKDESQRQEKAKQQAEDAWDRLPPNVRKFDVDVEQF
                     HYLVVLDDYGNVLSVTRTAVRPYVGSEKAKAGIMDKVDHKTPEEIYEALGFNNEEPQRQ
                     DQAKKAAYDVFYSFSMNRDRIQSDVLNKAAEVISDIGNKVGDYLGDDYKSLAREIADDV
                     KNFQGKTIRSYDDAMASLNKVLSNPGFKFNRADSDALANVWRSIDAQDMANKLGNISKA
                     FKFADVVMKVEKVREKSIEGYETGNWGPLMLEVESWVLSGIASAVALGVFSATLGAYAL
                     SLGAPAIAVGIVGILLAAVVGALIDDKFADALNKEIIKPAH"
                     /name="csa"
     gene            complement(1993..2532)
                     /gene="csi"
                     /locus_tag="H6S14_25500"
     CDS             complement(1993..2532)
                     /gene="csi"
                     /locus_tag="H6S14_25500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 immunity protein"
                     /protein_id="UIV31748.1"
                     /translation="MMIDEHSIDIDNRKANNLLYLFMVIGVIPLLCILAVYYTNPDNLF
                     LHTIATSTENIPSITSAYNPLMTKVMDIYCKTAPSLALILFILTFKTRKLIKKTNRNAV
                     LRSCLLSPFVCAVFLYLLCFRNLELTTAGRPARLMTNNDATLLLFYIGLYLIIFFISYF
                     TLFTPVTTFKLLKERQ"
                     /name="csi"
     gene            2562..2717
                     /gene="csl"
                     /locus_tag="H6S14_25505"
     CDS             2562..2717
                     /gene="csl"
                     /locus_tag="H6S14_25505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071703583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 system lysis protein"
                     /protein_id="UIV31749.1"
                     /translation="MKKIIICAISLGIMLLAACQVNNVRDTGGGAVSPSSTVTGVSMGS
                     GGSGSL"
                     /name="csl"
     gene            complement(3715..3906)
                     /locus_tag="H6S14_25510"
     CDS             complement(3715..3906)
                     /locus_tag="H6S14_25510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UIV31740.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDVDDQADICEALHDHA
                     DELYRSCLAHFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3974..4297
                     /locus_tag="H6S14_25515"
     CDS             3974..4297
                     /locus_tag="H6S14_25515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UIV31741.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4260..5003
                     /locus_tag="H6S14_25520"
                     /pseudo=""
     CDS             4260..5003
                     /locus_tag="H6S14_25520"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4975..5538
                     /locus_tag="H6S14_25525"
     CDS             4975..5538
                     /locus_tag="H6S14_25525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="UIV31742.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLIKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVERRKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            5545..5778
                     /gene="mbeD"
                     /locus_tag="H6S14_25530"
     CDS             5545..5778
                     /gene="mbeD"
                     /locus_tag="H6S14_25530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UIV31743.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="mbeD"
     gene            complement(6261..6683)
                     /gene="exc2"
                     /locus_tag="H6S14_25535"
     CDS             complement(6261..6683)
                     /gene="exc2"
                     /locus_tag="H6S14_25535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="UIV31744.1"
                     /translation="MRSAATRLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRAKEFHSEKFFHSLQSTTT
                     FAGRKYTNSDMPSLKKMKLMADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            6805..7083
                     /locus_tag="H6S14_25540"
     CDS             6805..7083
                     /locus_tag="H6S14_25540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UIV31745.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     Typing          3543..3629
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.864"
                     /coverage="99.0"
                     /evalue="1.15e-36"
     Typing          complement(2975..3519)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.796"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          4288..5885
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.874"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgggcgaggc gaatgcgtct tgcctggtat accgccgagt aagcctgggt gggattcaca
       61 ccattactga tttcactgac cacatgacgc tgatcagcca gtgtcagcgt gtaaacctgt
      121 ttctggatga ggtcacgggc tttgtcatgc tcatatccgg cagcaacaag ggtgtcgaga
      181 agtcgtttgt ctgttttgtt cagtttatgg cggggtgatt tgctcacatc gttctccgtt
      241 tttcatgatc caacagtttt atatctcatc ctgatttgct ccggtgttct gtttcgacgt
      301 tctcaatgcc tgcctcccca tagcgtgatc cttatagcaa aaatgaaaat ccatgctctt
      361 gacatggaca atggtgagta gtaggttttg ctgtacataa aaccagtggt tatatgtaca
      421 gtatactgtt tttaatttat tgttttaatt atcaaagagg aatttatggc taaagaacta
      481 agtggatatg gcccaactgc tggtgagtct atggggggaa caggggcgaa tctgaatcag
      541 caaggtggaa ataataatag taatagtggg gttcactggg gtggtggttc aggctctggt
      601 aatggaggtc gtgaacatgg aagtcagacg gggtggggat ggagtaaaac caataatccg
      661 gatgtgccgc cttatgttga tgataatggg caggtgcgca ttaccatcac aaatggtctg
      721 gtaaaaacgc ctgtatatgg agttcctggg gctggcggaa atagtgatgt tcaggggggc
      781 tatatccctg aaaatcctaa tgatgaagtt gctcgtaagt gggataaaaa caacttaccg
      841 cgagagattg atgtgagtat cgatggtttt aaatatcgtg taacgcttaa tgataatggt
      901 agagctatcg gtattcttcg aacaggtgtc cgtccttacg tagggagtga aaaagctaag
      961 gcgggtatta tggaaaaaat caatcataaa actcctgagg agatatatga ggcgttaggt
     1021 tttaataaag atgaatctca gcggcaggaa aaagcaaaac aacaggctga agatgcgtgg
     1081 gatcgtttgc ctcctaatgt gagaaaattt gatgttgatg ttgaacagtt ccactattta
     1141 gtggtgcttg atgattatgg taatgtttta tctgtaacta gaacagctgt tcgtccttat
     1201 gtagggagtg aaaaagctaa ggctggtatt atggataaag tcgaccataa gaccccggaa
     1261 gagatatatg aggcattagg atttaataat gaagagcctc aacgacagga tcaagctaaa
     1321 aaagctgctt atgatgtgtt ttattctttc agtatgaacc gtgaccggat tcagagtgat
     1381 gtacttaata aagccgctga agttatctct gatataggta ataaggtcgg tgattatctt
     1441 ggagatgatt ataagtcgtt agcaagagaa attgctgatg atgtcaaaaa tttccagggg
     1501 aaaactatcc gtagctacga tgatgcaatg gcgtcattaa ataaagtttt gtctaatccg
     1561 gggtttaaat ttaacagggc cgacagtgat gcactggcga acgtctggcg aagtattgat
     1621 gctcaggata tggcaaataa actgggtaat atcagcaagg ctttcaaatt tgccgatgtg
     1681 gtgatgaagg ttgagaaggt ccgggagaaa agcatcgagg gctatgaaac cgggaactgg
     1741 ggacctctga tgctggaggt ggagtcctgg gtgctcagtg gcatcgcttc tgctgtggcc
     1801 ctgggtgttt tttcagccac attaggggca tatgccttat ctcttggggc tcctgctatt
     1861 gctgtcggta tcgtaggtat tctactcgca gcagttgtag gtgcattaat tgatgacaag
     1921 tttgcagatg cactaaataa agaaatcatt aaacccgcgc attaataaat accgggtaaa
     1981 aggaaggcag ggttactgcc tttcttttaa taatttaaac gtggtaactg gtgtgaatag
     2041 cgtgaaatag cttatgaaaa aaatgattaa gtacaggcca atataaaata gcaatagtgt
     2101 tgcatcattg ttggtcatta gcctggctgg ccttcctgct gtcgttagct caaggtttcg
     2161 gaaacagagt aaatacaaaa aaacagcaca gacaaatgga cttaataagc aacttctaag
     2221 tacagcattt cggtttgttt tttttattag ctttctcgtt ttaaatgtca ggataaataa
     2281 tatcaaagca agggaaggcg ctgttttaca ataaatatcc ataacctttg tcattaatgg
     2341 gttgtaggcg gaggttattg atgggatgtt ttcagtactg gtagctattg tgtgcaagaa
     2401 cagattgtct ggatttgtat agtaaacggc tagtatacag agtaaaggta taactccgat
     2461 taccataaat aaatacaata agttattggc ttttctattg tctatatcta ttgaatgttc
     2521 atcaatcatc attgacctat aggattataa taaaggtata tatgaaaaaa attattattt
     2581 gtgccatttc actaggaata atgctgcttg cagcatgcca agtcaacaat gtcagagata
     2641 ctggaggtgg ggctgtttca ccatcttcca ctgttaccgg agtgagtatg ggaagtggtg
     2701 gtagtggtag cctgtaaagg atcttcttga gatcctttac agatcgtcgt agtctcttgc
     2761 cctgtaaacg aaaaaaccgc ctggggaggc ggtttagtcg aaggttaagt cagttgggga
     2821 ctgcttaacc gggtagctgg cttaagtgga gcacagatac caaatgctgt cctttcagtg
     2881 tagccgctat aaggcttcaa cttcaagagt tctgcgtatc taaatccact tttcagttac
     2941 caatggctgc tgccagtggc gctttgtcgt gtcttaccgg ggtggactcg agacgatagt
     3001 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     3061 agcgaacgac ctacaccgaa ctgagatacc aacagcgtga gctatgagaa agcgccacgc
     3121 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     3181 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     3241 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     3301 acgccagcga cgcggctttt tttgcccttt gcctttttgc tcacatgttc ttttctgcct
     3361 tatcccctga ttctgtggat aaccgtatcg ccgtttttga gcaagcagac tccgctcgcc
     3421 gcagtcgaac gaccgagcgc agcgagtcag tgagcgagga agcggaaaag tgcccggacg
     3481 agcattttct ccttacgcat ctgtgcggca tttcacaccc ggcatggtgc acttttcata
     3541 caatcctcac tgatgccaca tgattaagcc agtatacact ccgctatcgc tacgtgactg
     3601 ggtcatggct gcgccccgac acccgctaac acccgctgac gcgccctgac gggcttgtct
     3661 gctcccggca tccgctcaca ggccagctgt gaccgtctcc gggagctgca tgtgtcagag
     3721 gttttcaccg tcatccccga agtgtgcgag gcagctgcgg taaagctcgt cagcgtggtc
     3781 gtgaagcgct tcgcagatgt cagcctggtc gtcgacgtcc agttcattca gtttttccag
     3841 cagggtcagc gtctggcttc tgatgaagcg ggccatgtta agggcggttt tttcctgttt
     3901 agtcacagtt ctgtttctcc atgtgtgggg agtctgtcgg aggtttttaa tgataccggt
     3961 gaaacgcgaa aggatgctca cgatacgggt cacggatgag gaacatgcgc ggctgctggc
     4021 acgttgtgag ggaaaacaac ttgcgtcatg gatgcgtaag gtctgcctgg gggcaccgcc
     4081 atcgaaaacg tcaggtcttc cgacactggc accgccgctg ttgcgccagt tcgcttcggt
     4141 cggcaataac ctgaatcaga tagcccggaa gataaacagc ggacagtggt cggggcatga
     4201 ccgggttcat gtggtggctg cactgatggc catcggacgt gaactgagtg agcttaggga
     4261 tgaggtccgt aaacagggag aacgggatga tagttaagtt tcatgccagg ggaaaaggcg
     4321 gtggcagtgg tccggttgat tacctgctgg gccgggagcg taaccgggaa ggggtgagag
     4381 ttctgcgggg tgctcccgaa gaggtacggg aactcatcga tgccacgccg tttgcgaaaa
     4441 aatacacctc gggggttctg tcttttgcag agcagaccct gccaccggga gagcgtgaaa
     4501 gggtgatgga gagctttgag cgggtactga tgcccggtct tgaaaaaaat cagtacagca
     4561 tcctgtgggt ggagcaccag gacaaggggc gtcttgagct gaattttgtc atcccgaata
     4621 tggagctggc gagcggaaaa cggctccagc cctattacga ccgggcggac cgtccgcgaa
     4681 tcaatgcctg gcagacactg gtgaatcacc attacgggct gcacgacccg aacgcaccgg
     4741 agaacaggcg gacacttgtc accccgaata atctgccgaa agcgaaacag gaggccgcag
     4801 aagcgattac gaggggactg gaggctcttt accatgccgg agcgctaaaa acgcgtcagg
     4861 atgtcactga ggcgcttaca gcggcaggtt ttgaggttgt gaggacaacc aggagcagta
     4921 tcagtatcgc cgacccggag ggcgggcgta atctcaggct gaaaggggcg ctgtatgagc
     4981 aatcttttac aaatggggac agacttcgag aagaaactga aagagcgtgc cgtctctaca
     5041 gagaacatgc tgaacagcga gttcaggaag ctcgaagcgt ctgtagacag ggaactgaac
     5101 ttaaacgggc agaaaatcag agacgctatc agcgcacaca cagcagcggt gaaagaacaa
     5161 ctggagacgc tgaacagtgc cgtgaacgcg cagttcagcg ccacagagaa cgaactggcc
     5221 cggcggcagg aagagctgct ctggaagctg atcaaagggc ggatactgta cccgtcgctg
     5281 acggcgcttt gcgtcacggg cgggattttt ctggcgtcat gggggctgat tcagtggcag
     5341 gaaagccgga ttgcggcgaa catcctggct atcagggacc aggaagagac gctggcgaaa
     5401 ctccgggaaa aaacatgggg tgtgacgtat cacgagggca ggaacgggaa atttctggtg
     5461 ctgccgtccg gggtgaaggg ggaaaacaac tggacggtgg agagaagaaa gaacgcagta
     5521 agactggtca gggagtaagt gagtatgaca gagctggaaa tgcacttact gaacgcctta
     5581 gagcaattgc agcaggacta tacgcagcgg ctgaaagact gggagagcgc cttcacggat
     5641 ttgcagcata tgttcgagct gacacgacag gagaacgggc aactgaggga acagtgcgac
     5701 gacttgagtc agcaggtgca gagattagcc gggcaggtgg accgcttgag tcggttattt
     5761 atcacgaaca gcagataagg gatgaacagg aaaggcaggc acgcctggaa cgggagcgtg
     5821 cgcggtcctg gcatgaagag caggaacggg aagcacgggt atggcgtggc ccgtcaatgg
     5881 ggatgtgacg gtgagttgtt ccgggaggtt gccagatggt accggaacgc gttaaaaacg
     5941 catccgggcg cgattctgga gtgtcgtttt tgttgctgtg ttttgcctct ttgccagatc
     6001 gcgctcattc ctcagcctca tcccgcgatg tctgcgtatg tcatcaactg aaaagatgga
     6061 aaaaaacagt gtcatgaggt ttacgcaggc aaaaccagcg ttattcacat ggctgaatag
     6121 ctatgcatac ggttaaaatt tatcaggtgc gatcgcggca gcttttcggg tggtttgttg
     6181 ccatttttac ctgtcagcag ccgtgatcgc gctgaacgca tttcagcggt gcgtacaatt
     6241 aagggattat ggtaaatccc ttactgtctg ccctcgtatc catcgagata aaccgcagaa
     6301 atggtgtctg ccattagttt catttttttc agcgaaggca tatcgctgtt tgtgtatttt
     6361 ctgccagcaa aggttgttgt gctctggagt gagtgaaaaa atttttctga gtggaattct
     6421 tttgcacgtt gctgtgccgt ttctgctgat actccggctg ctttgtcttt catccccata
     6481 tcccggaact gctgaaagaa cgggagcatc atacgtatgg tgtctgcttt ctgggtatag
     6541 aagttaggat caaatccatc atctgaagca taaacataat gtcgggtatg tcgttccagg
     6601 ctggtctttg gggtgcatcc tgtaagagct attgttgctg ttatcagagg tatcagaagc
     6661 agcctggtag cggcagatct cataaacatc tccttgcgtt tttaatcttg atactcaggg
     6721 agttatatct ccgaagagta tctcttatca tacgtcggtt tactcccaat gtgatttgat
     6781 ggtcaatgag tcaggaataa tcccgtgaaa aaatgtgtgc cagtcctgct ttttagtgtt
     6841 ctttctgctt cggttgccag tcatgcggac agcaatgttt ttcaacaaaa ggccgagaag
     6901 acaaaaaacg gcattgttgc ggatgttaat gccagacaga aaatgttcag taacagactg
     6961 aagatacaac ttcagatcga tgagttgaga gcagagctgg ctggcaccag agatgcccaa
     7021 aaaagagagg atttgcgtaa aaaactggag tctctggagc ggcaaaagaa atccttacgt
     7081 tgatgggggc gtattttccc cggtttcttc cataaccttt ttaccgttca gatattta
//
