LOCUS       CP061225                7135 bp    DNA     circular BCT 13-JAN-2022
DEFINITION  Escherichia coli strain STEC403 plasmid pSTEC403_2, complete
            sequence.
ACCESSION   CP061225
VERSION     CP061225.1
DBLINK      BioProject: PRJNA655129
            BioSample: SAMN15748004
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7135)
  AUTHORS   Pan,Y., Xiong,Y. and Yang,X.
  TITLE     Genomic characteristics of Shiga toxin-producing Escherichia coli
            (STEC)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7135)
  AUTHORS   Pan,Y., Xiong,Y. and Yang,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2020) National Institute for Communicable Disease
            Control and Prevention, Chinese Center for Disease Control and
            Prevention, No. 155, Changbai Rd, Beijing 102200, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. v. 4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 09/02/2020 11:42:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.12
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,110
            CDSs (total)                      :: 4,987
            Genes (coding)                    :: 4,849
            CDSs (with protein)               :: 4,849
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 57 of 138
            Pseudo Genes (incomplete)         :: 67 of 138
            Pseudo Genes (internal stop)      :: 41 of 138
            Pseudo Genes (multiple problems)  :: 23 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="STEC403"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSTEC403_2"
                     /geo_loc_name="China: Henan"
                     /collection_date="2013"
     gene            complement(join(6207..7135,1..571))
                     /gene="csa"
                     /locus_tag="H6S17_25500"
     CDS             complement(join(6207..7135,1..571))
                     /gene="csa"
                     /locus_tag="H6S17_25500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin S4"
                     /protein_id="UIV18728.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGSG
                     NGGREHGSQTGWGWSKTNNPDVPPYVDDNGQVRITITNGLVKTPVYGVPGAGGNSDVQG
                     GYIPENPNDEVARKWDKNNLPREIDVSIDGFKYRVTLNDNGRAIGILRTGVRPYVGSEK
                     AKAGIMEKINHKTPEEIYEALGFNKDESQRQEKAKQQAEDAWDRLPPNVRKFDVDVEQF
                     HYLVVLDDYGNVLSVTRTAVRPYVGSEKAKAGIMDKVDHKTPEEIYEALGFNNEEPQRQ
                     DQAKKAAYDVFYSFSMNRDRIQSDVLNKAAEVISDIGNKVGDYLGDDYKSLAREIADDV
                     KNFQGKTIRSYDDAMASLNKVLSNPGFKFNRADSDALANVWRSIDAQDMANKLGNISKA
                     FKFADVVMKVEKVREKSIEGYETGNWGPLMLEVESWVLSGIASAVALGVFSATLGAYAL
                     SLGAPAIAVGIVGILLAAVVGALIDDKFADALNKEIIKPAH"
                     /name="csa"
     gene            810..1100
                     /locus_tag="H6S17_25505"
     CDS             810..1100
                     /locus_tag="H6S17_25505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UIV18729.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPST"
                     /name="hypothetical protein"
     gene            complement(1092..1370)
                     /locus_tag="H6S17_25510"
     CDS             complement(1092..1370)
                     /locus_tag="H6S17_25510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UIV18730.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            1492..1914
                     /gene="exc2"
                     /locus_tag="H6S17_25515"
     CDS             1492..1914
                     /gene="exc2"
                     /locus_tag="H6S17_25515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="UIV18731.1"
                     /translation="MRSAATRLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRAKEFHSEKFFHSLQSTTT
                     FAGRKYTNSDMPSLKKMKLMADTISAVYLDGYEGRQ"
                     /name="exc2"
     gene            complement(2397..2630)
                     /gene="mbeD"
                     /locus_tag="H6S17_25520"
     CDS             complement(2397..2630)
                     /gene="mbeD"
                     /locus_tag="H6S17_25520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UIV18732.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="mbeD"
     gene            complement(2637..3197)
                     /locus_tag="H6S17_25525"
     CDS             complement(2637..3197)
                     /locus_tag="H6S17_25525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="UIV18733.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLIKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(3169..3912)
                     /locus_tag="H6S17_25530"
                     /pseudo=""
     CDS             complement(3169..3912)
                     /locus_tag="H6S17_25530"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3875..4198)
                     /locus_tag="H6S17_25535"
     CDS             complement(3875..4198)
                     /locus_tag="H6S17_25535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UIV18724.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4266..4457
                     /locus_tag="H6S17_25540"
     CDS             4266..4457
                     /locus_tag="H6S17_25540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UIV18725.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDVDDQADICEALHDHA
                     DELYRSCLAHFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5455..5610)
                     /gene="csl"
                     /locus_tag="H6S17_25545"
     CDS             complement(5455..5610)
                     /gene="csl"
                     /locus_tag="H6S17_25545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071703583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 system lysis protein"
                     /protein_id="UIV18726.1"
                     /translation="MKKIIICAISLGIMLLAACQVNNVRDTGGGAVSPSSTVTGVSMGS
                     GGSGSL"
                     /name="csl"
     gene            5640..6179
                     /gene="csi"
                     /locus_tag="H6S17_25550"
     CDS             5640..6179
                     /gene="csi"
                     /locus_tag="H6S17_25550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 immunity protein"
                     /protein_id="UIV18727.1"
                     /translation="MMIDEHSIDIDNRKANNLLYLFMVIGVIPLLCILAVYYTNPDNLF
                     LHTIATSTENIPSITSAYNPLMTKVMDIYCKTAPSLALILFILTFKTRKLIKKTNRNAV
                     LRSCLLSPFVCAVFLYLLCFRNLELTTAGRPARLMTNNDATLLLFYIGLYLIIFFISYF
                     TLFTPVTTFKLLKERQ"
                     /name="csi"
     Typing          complement(4544..4630)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.864"
                     /coverage="99.0"
                     /evalue="1.15e-36"
     Typing          4654..5198
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.796"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2291..3885)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.248"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4258..4460)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="86.765"
                     /coverage="100.0"
                     /evalue="1.39e-10"
ORIGIN
        1 attcatcttt attaaaacct aacgcctcat atatctcctc aggagtttta tgattgattt
       61 tttccataat acccgcctta gctttttcac tccctacgta aggacggaca cctgttcgaa
      121 gaataccgat agctctacca ttatcattaa gcgttacacg atatttaaaa ccatcgatac
      181 tcacatcaat ctctcgcggt aagttgtttt tatcccactt acgagcaact tcatcattag
      241 gattttcagg gatatagccc ccctgaacat cactatttcc gccagcccca ggaactccat
      301 atacaggcgt ttttaccaga ccatttgtga tggtaatgcg cacctgccca ttatcatcaa
      361 cataaggcgg cacatccgga ttattggttt tactccatcc ccaccccgtc tgacttccat
      421 gttcacgacc tccattacca gagcctgaac caccacccca gtgaacccca ctattactat
      481 tattatttcc accttgctga ttcagattcg cccctgttcc ccccatagac tcaccagcag
      541 ttgggccata tccacttagt tctttagcca taaattcctc tttgataatt aaaacaataa
      601 attaaaaaca gtatactgta catataacca ctggttttat gtacagcaaa acctactact
      661 caccattgtc catgtcaaga gcatggattt tcatttttgc tataaggatc acgctatggg
      721 gaggcaggca ttgagaacgt cgaaacagaa caccggagca aatcaggatg agatataaaa
      781 ctgttggatc atgaaaaacg gagaacgatg tgagcaaatc accccgccat aaactgaaca
      841 aaacagacaa acgacttctc gacacccttg ttgctgccgg atatgagcat gacaaagccc
      901 gtgacctcat ccagaaacag gtttacacgc tgacactggc tgatcagcgt catgtggtca
      961 gtgaaatcag taatggtgtg aatcccaccc aggcttactc ggcggtatac caggcaagac
     1021 gcattcgcct cgcccgtaaa tatctgaacg gtaaaaaggt tatggaagaa accggggaaa
     1081 atacgccccc atcaacgtaa ggatttcttt tgccgctcca gagactccag ttttttacgc
     1141 aaatcctctc ttttttgggc atctctggtg ccagccagct ctgctctcaa ctcatcgatc
     1201 tgaagttgta tcttcagtct gttactgaac attttctgtc tggcattaac atccgcaaca
     1261 atgccgtttt ttgtcttctc ggccttttgt tgaaaaacat tgctgtccgc atgactggca
     1321 accgaagcag aaagaacact aaaaagcagg actggcacac attttttcac gggattattc
     1381 ctgactcatt gaccatcaaa tcacattggg agtaaaccga cgtatgataa gagatactct
     1441 tcggagatat aactccctga gtatcaagat taaaaacgca aggagatgtt tatgagatct
     1501 gccgctacca ggctgcttct gatacctctg ataacagcaa caatagctct tacaggatgc
     1561 accccaaaga ccagcctgga acgacatacc cgacattatg tttatgcttc agatgatgga
     1621 tttgatccta acttctatac ccagaaagca gacaccatac gtatgatgct cccgttcttt
     1681 cagcagttcc gggatatggg gatgaaagac aaagcagccg gagtatcagc agaaacggca
     1741 cagcaacgtg caaaagaatt ccactcagaa aaattttttc actcactcca gagcacaaca
     1801 acctttgctg gcagaaaata cacaaacagc gatatgcctt cgctgaaaaa aatgaaacta
     1861 atggcagaca ccatttctgc ggtttatctc gatggatacg agggcagaca gtaagggatt
     1921 taccataatc ccttaattgt acgcaccgct gaaatgcgtt cagcgcgatc acggctgctg
     1981 acaggtaaaa atggcaacaa accacccgaa aagctgccgc gatcgcacct gataaatttt
     2041 aaccgtatgc atagctattc agccatgtga ataacgctgg ttttgcctgc gtaaacctca
     2101 tgacactgtt tttttccatc ttttcagttg atgacatacg cagacatcgc gggatgaggc
     2161 tgaggaatga gcgcgatctg gcaaagaggc aaaacacagc aacaaaaacg acactccaga
     2221 atcgcgcccg gatgcgtttt taacgcgttc cggtaccatc tggcaacctc ccggaacaac
     2281 tcaccgtcac atccccattg acgggccacg ccatacccgt gcttcccgtt cctgctcttc
     2341 atgccaggac cgcgcacgct cccgttccag gcgtgcctgc ctttcctgtt catcccttat
     2401 ctgctgttcg tgataaataa ccgactcaag cggtccacct gcccggctaa tctctgcacc
     2461 tgctgactca agtcgtcgca ctgttccctc agttgcccgt tctcctgtcg tgtcagctcg
     2521 aacatatgct gcaaatccgt gaaggcgctc tcccagtctt tcagccgctg cgtatagtcc
     2581 tgctgcaatt gctctaaggc gttcagtaag tgcatttcca gctctgtcat actcacttac
     2641 tccctgacca gtcttactgc gttcttcttc tccaccgtcc agttgttttc ccccttcacc
     2701 ccggacggca gcaccagaaa tttcccgttc ctgccctcgt gatacgtcac accccatgtt
     2761 ttttcccgga gtttcgccag cgtctcttcc tggtccctga tagccaggat gttcgccgca
     2821 atccggcttt cctgccactg aatcagcccc catgacgcca gaaaaatccc gcccgtgacg
     2881 caaagcgccg tcagcgacgg gtacagtatc cgccctttga tcagcttcca gagcagctct
     2941 tcctgccgcc gggccagttc gttctctgtg gcgctgaact gcgcgttcac ggcactgttc
     3001 agcgtctcca gttgttcttt caccgctgct gtgtgtgcgc tgatagcgtc tctgattttc
     3061 tgcccgttta agttcagttc cctgtctaca gacgcttcga gcttcctgaa ctcgctgttc
     3121 agcatgttct ctgtagagac ggcacgctct ttcagtttct tctcgaagtc tgtccccatt
     3181 tgtaaaagat tgctcataca gcgccccttt cagcctgaga ttacgcccgc cctccgggtc
     3241 ggcgatactg atactgctcc tggttgtcct cacaacctca aaacctgccg ctgtaagcgc
     3301 ctcagtgaca tcctgacgcg tttttagcgc tccggcatgg taaagagcct ccagtcccct
     3361 cgtaatcgct tctgcggcct cctgtttcgc tttcggcaga ttattcgggg tgacaagtgt
     3421 ccgcctgttc tccggtgcgt tcgggtcgtg cagcccgtaa tggtgattca ccagtgtctg
     3481 ccaggcattg attcgcggac ggtccgcccg gtcgtaatag ggctggagcc gttttccgct
     3541 cgccagctcc atattcggga tgacaaaatt cagctcaaga cgccccttgt cctggtgctc
     3601 cacccacagg atgctgtact gatttttttc aagaccgggc atcagtaccc gctcaaagct
     3661 ctccatcacc ctttcacgct ctcccggtgg cagggtctgc tctgcaaaag acagaacccc
     3721 cgaggtgtat tttttcgcaa acggcgtggc atcgatgagt tcccgtacct cttcgggagc
     3781 accccgcaga actctcaccc cttcccggtt acgctcccgg cccagcaggt aatcaaccgg
     3841 accactgcca ccgccttttc ccctggcatg aaacttaact atcatcccgt tctccctgtt
     3901 tacggacctc atccctaagc tcactcagtt cacgtccgat ggccatcagt gcagccacca
     3961 catgaacccg gtcatgcccc gaccactgtc cgctgtttat cttccgggct atctgattca
     4021 ggttattgcc gaccgaagcg aactggcgca acagcggcgg tgccagtgtc ggaagacctg
     4081 acgttttcga tggcggtgcc cccaggcaga ccttacgcat ccatgacgca agttgttttc
     4141 cctcacaacg tgccagcagc cgcgcatgtt cctcatccgt gacccgtatc gtgagcatcc
     4201 tttcgcgttt caccggtatc attaaaaacc tccgacagac tccccacaca tggagaaaca
     4261 gaactgtgac taaacaggaa aaaaccgccc ttaacatggc ccgcttcatc agaagccaga
     4321 cgctgaccct gctggaaaaa ctgaatgaac tggacgtcga cgaccaggct gacatctgcg
     4381 aagcgcttca cgaccacgct gacgagcttt accgcagctg cctcgcacac ttcggggatg
     4441 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagctggc ctgtgagcgg
     4501 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttagcggg tgtcggggcg
     4561 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaatc atgtggcatc
     4621 agtgaggatt gtatgaaaag tgcaccatgc cgggtgtgaa atgccgcaca gatgcgtaag
     4681 gagaaaatgc tcgtccgggc acttttccgc ttcctcgctc actgactcgc tgcgctcggt
     4741 cgttcgactg cggcgagcgg agtctgcttg ctcaaaaacg gcgatacggt tatccacaga
     4801 atcaggggat aaggcagaaa agaacatgtg agcaaaaagg caaagggcaa aaaaagccgc
     4861 gtcgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     4921 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     4981 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     5041 cccttcggga agcgtggcgc tttctcatag ctcacgctgt tggtatctca gttcggtgta
     5101 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     5161 cttatccggt aactatcgtc tcgagtccac cccggtaaga cacgacaaag cgccactggc
     5221 agcagccatt ggtaactgaa aagtggattt agatacgcag aactcttgaa gttgaagcct
     5281 tatagcggct acactgaaag gacagcattt ggtatctgtg ctccacttaa gccagctacc
     5341 cggttaagca gtccccaact gacttaacct tcgactaaac cgcctcccca ggcggttttt
     5401 tcgtttacag ggcaagagac tacgacgatc tgtaaaggat ctcaagaaga tcctttacag
     5461 gctaccacta ccaccacttc ccatactcac tccggtaaca gtggaagatg gtgaaacagc
     5521 cccacctcca gtatctctga cattgttgac ttggcatgct gcaagcagca ttattcctag
     5581 tgaaatggca caaataataa tttttttcat atataccttt attataatcc tataggtcaa
     5641 tgatgattga tgaacattca atagatatag acaatagaaa agccaataac ttattgtatt
     5701 tatttatggt aatcggagtt atacctttac tctgtatact agccgtttac tatacaaatc
     5761 cagacaatct gttcttgcac acaatagcta ccagtactga aaacatccca tcaataacct
     5821 ccgcctacaa cccattaatg acaaaggtta tggatattta ttgtaaaaca gcgccttccc
     5881 ttgctttgat attatttatc ctgacattta aaacgagaaa gctaataaaa aaaacaaacc
     5941 gaaatgctgt acttagaagt tgcttattaa gtccatttgt ctgtgctgtt tttttgtatt
     6001 tactctgttt ccgaaacctt gagctaacga cagcaggaag gccagccagg ctaatgacca
     6061 acaatgatgc aacactattg ctattttata ttggcctgta cttaatcatt tttttcataa
     6121 gctatttcac gctattcaca ccagttacca cgtttaaatt attaaaagaa aggcagtaac
     6181 cctgccttcc ttttacccgg tatttattaa tgcgcgggtt taatgatttc tttatttagt
     6241 gcatctgcaa acttgtcatc aattaatgca cctacaactg ctgcgagtag aatacctacg
     6301 ataccgacag caatagcagg agccccaaga gataaggcat atgcccctaa tgtggctgaa
     6361 aaaacaccca gggccacagc agaagcgatg ccactgagca cccaggactc cacctccagc
     6421 atcagaggtc cccagttccc ggtttcatag ccctcgatgc ttttctcccg gaccttctca
     6481 accttcatca ccacatcggc aaatttgaaa gccttgctga tattacccag tttatttgcc
     6541 atatcctgag catcaatact tcgccagacg ttcgccagtg catcactgtc ggccctgtta
     6601 aatttaaacc ccggattaga caaaacttta tttaatgacg ccattgcatc atcgtagcta
     6661 cggatagttt tcccctggaa atttttgaca tcatcagcaa tttctcttgc taacgactta
     6721 taatcatctc caagataatc accgacctta ttacctatat cagagataac ttcagcggct
     6781 ttattaagta catcactctg aatccggtca cggttcatac tgaaagaata aaacacatca
     6841 taagcagctt ttttagcttg atcctgtcgt tgaggctctt cattattaaa tcctaatgcc
     6901 tcatatatct cttccggggt cttatggtcg actttatcca taataccagc cttagctttt
     6961 tcactcccta cataaggacg aacagctgtt ctagttacag ataaaacatt accataatca
     7021 tcaagcacca ctaaatagtg gaactgttca acatcaacat caaattttct cacattagga
     7081 ggcaaacgat cccacgcatc ttcagcctgt tgttttgctt tttcctgccg ctgag
//
