LOCUS       CP060797                5144 bp    DNA     circular BCT 30-MAR-2021
DEFINITION  Enterococcus faecalis strain EF-2001 plasmid pEFSP1, complete
            sequence.
ACCESSION   CP060797
VERSION     CP060797.1
DBLINK      BioProject: PRJNA658454
            BioSample: SAMN15871855
KEYWORDS    .
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Panthee,S., Paudel,A., Hamamoto,H. and Sekimizu,K.
  TITLE     Complete genome sequence of Enterococcus faecalis EF-2001
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5144)
  AUTHORS   Panthee,S., Paudel,A., Hamamoto,H. and Sekimizu,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2020) TIMM, Teikyo University Institute of Medical
            Mycology, Otsuka 359, Hachioji, Tokyo 192-0395, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2020
            Assembly Method                   :: Flye v. 2.8
            Assembly Name                     :: EF-2001
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1000.0x
            Sequencing Technology :: Oxford Nanopore MiniION; IonTorrent
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/31/2020 10:59:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.12
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 2,741
            CDSs (total)                      :: 2,661
            Genes (coding)                    :: 2,544
            CDSs (with protein)               :: 2,544
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 91 of 117
            Pseudo Genes (incomplete)         :: 17 of 117
            Pseudo Genes (internal stop)      :: 13 of 117
            Pseudo Genes (multiple problems)  :: 4 of 117
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5144
                     /organism="Enterococcus faecalis"
                     /mol_type="genomic DNA"
                     /strain="EF-2001"
                     /host="Homo sapiens"
                     /db_xref="taxon:1351"
                     /plasmid="pEFSP1"
                     /geo_loc_name="Japan"
                     /collection_date="2001"
     gene            complement(join(5089..5144,1..1624))
                     /locus_tag="H8446_13435"
     CDS             complement(join(5089..5144,1..1624))
                     /locus_tag="H8446_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="QTI54052.1"
                     /translation="MSKIVARMEKMKDGNLSGIQRHNQRETNNHSNPDIDIEKSHLNYD
                     LVNPGSINYREKIKQIIESQRISKRAVRKDAVLVNEWIITSDTAFFQENTDTQAFFTDV
                     VAYFSDRCGRQNVAYATVHLDETTPHMHLGIVPMYEGRLSSKQVFSRQNLLEIQEELPT
                     YLQERGYAIERGLRGSPQKHLSVKEYKDVQKELQVSKQELANQEQILAEKERELHSYEQ
                     AIFRIDRLLEEKNEEVKGKVQAMNQLETKRNEIINSLQTTVLPDLANVKEKKFSLNGLK
                     YVLSASDFSAIKSITKDFETLRTQNKHLKIENNNLAQKNKELTQVNHEQELQITELAYE
                     LTLKKEQLTAFEEKQLNYDQSEQIKQELDTTTQTVSVLETENKRLKDKITDLTYDFSEL
                     NEKYQEIKLKFDGLTTYLHERLGHAKETILFTIQQGVKYLQNAPKLKKSLERYSKDRTY
                     LIDEKGTLFLPPDTGSKGVTMSLNPHFYPLKAPFYNRETGCYEFVGEYMANGRSRTNTL
                     QLSEEQVLEKKAFTFEQSTDKKAWQQSKKYSRDMSKGRGLSR"
                     /name="plasmid recombination protein"
     gene            complement(2403..2699)
                     /locus_tag="H8446_13440"
     CDS             complement(2403..2699)
                     /locus_tag="H8446_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002404941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QTI54053.1"
                     /translation="MGLMTGYNMTKEEYEVTQAVLERLDKFESELEALLLDLEPLDLSR
                     LDTVVRDETLRMFNYLSRSYILLIDTVAYKVTLEEDISCGIVKIDGQSLDEEE"
                     /name="hypothetical protein"
     gene            complement(2826..3548)
                     /locus_tag="H8446_13445"
     CDS             complement(2826..3548)
                     /locus_tag="H8446_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QTI54050.1"
                     /translation="MSDKSELAVKYQNELNLVPLKNFNAKQMDLFFALCARMKDKGVEN
                     IRFSFEELKELSDYKMTATKAFVADLEKLYKDMLNLSYRTENDDEIEYFVLFNGFKIDK
                     KQKFVEVRVNQDLDYIINGLTTEFSRFELSAFTSIRSTYAKTLFRLLMQYRSTGYYVVS
                     IEDFRELLDIPEYYQMGNIDQKVLKPAMKELHNYFENLEVTKIKAKKGNKIAKLEFTFT
                     GLKTNKPSVTMHDWVNGE"
                     /name="replication initiation protein"
     gene            4195..4281
                     /locus_tag="H8446_13450"
     CDS             4195..4281
                     /locus_tag="H8446_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="QTI54054.1"
                     /translation="MIESLISLIIAPLFVGLVLLLVDRWLDD"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(4800..5000)
                     /locus_tag="H8446_13455"
     CDS             complement(4800..5000)
                     /locus_tag="H8446_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QTI54051.1"
                     /translation="MKKLLVLILGVSFISVALSSTTEASAREWDSIECQPGFYWKKGGW
                     TAGGYHKAGGFCIPMHTRWTK"
                     /name="hypothetical protein"
     Typing          1912..2171
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="80.0"
                     /coverage="81.0"
                     /evalue="1.95e-45"
     Typing          complement(2827..3548)
                     /name="rep_cluster_2301"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3..1684)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.695"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 gccaagcctt tttatctgtc gactgttcaa acgtaaaagc tttcttctct agcacttgtt
       61 cttcagacag ttgtaaagta ttcgttcgag aacgtccatt agccatatat tcgccgacaa
      121 actcataaca ccctgtttct ctgttgtaaa acggggcttt tagggggtag aaatgtgggt
      181 ttaaggacat tgttacccct tttgaccccg tatctggtgg taaaaacaat gtcccttttt
      241 cgtcaatgag gtaagttcta tccttggaat atcgctctag ggattttttt aatttaggcg
      301 cgttctgaag gtacttcact ccttgctgaa tcgtaaataa aatggtttct ttagcatgac
      361 ctagtctttc gtgaaggtaa gtagttaacc cgtcaaactt taattttatt tcttgatatt
      421 tttcatttag ttcagaaaag tcgtaggtta agtcagtaat tttatctttt aaccgcttat
      481 tctctgtttc taacacagaa actgtttgtg ttgttgtatc aagttcttgt ttgatttgtt
      541 cgctctggtc atagtttagt tgtttttctt caaacgctgt taattgttct tttttcaggg
      601 ttaattcata ggctaattcc gttatttgta attcttgttc atgatttact tgtgtcaatt
      661 ctttattttt ctgtgccaag ttattgtttt cgattttaag gtgcttattt tgcgttctaa
      721 gcgtctcgaa atctttcgta atacttttta tcgccgaaaa gtcagaagcg cttaaaacgt
      781 attttaaacc gtttaaacta aatttctttt ctttgacatt tgctaaatca ggtaaaacgg
      841 ttgtttgtag agagttaatg atttcgtttc gcttcgtttc cagttggttc attgcttgga
      901 ctttgccctt tacttcttcg ttcttctctt caagaaggcg gtcaatacgg aagattgcct
      961 gttcataaga atggagttct ctctcttttt ctgccagaat ttgttcctga ttagcgagtt
     1021 cctgcttaga aacttgtagc tccttttgaa cgtctttgta ttctttgaca gataaatgct
     1081 tttgcggact cccacggagt ccacgctcaa tagcatagcc tcgttcttgc aaataggttg
     1141 gcaattcttc ttgaatctct aacaaatttt gccgactaaa cacctgttta ctgctcaatc
     1201 gcccttcgta cataggcaca attcctaagt gcatatgggg cgtggtttcg tctaaatgta
     1261 ccgtggcata ggcgacattt tgtcgaccgc agcgatcaga gaaatatgcg acaacatcgg
     1321 taaaaaatgc ttgtgtgtct gtattctctt gaaaaaaggc ggtatcacta gtgattatcc
     1381 attcgttcac aaggactgcg tcttttctaa ccgctcgttt actgattcgt tggctctcaa
     1441 tgatttgttt gattttctcc cgataattga ttgaaccagg attgactaag tcataattca
     1501 agtgagattt ctcaatatca atatcaggat tggaatgatt attggtttct cgttgattat
     1561 gtcgttggat accacttaaa tttccgtctt tcattttttc catacgtgca acaatttttg
     1621 acatgagcgc tcctccccct ttttttttct tgtgtcgttc ggtgtctttg gatttcggca
     1681 gtacttccaa gtaaagtata acgcactata ctttacttcg taaaggtgcg ctctgccgag
     1741 ggctgtcggc tagtactcga aaggtcagcc tttaggcttc ccaatctcgc acttagccga
     1801 ttgcctacgg tcaaactttc catttctgct aaggcagaaa ctccaaattt ccctccgtca
     1861 gaaaaccgtt taaataacga ctgcctgaaa ttagtgaacg tgctagcttt ctgctagccg
     1921 gtgctagtaa tgtgctaatt tggtgctaaa aacctgctag tcactgctag gaatctgcta
     1981 gtttgggtgc tagaaacctg ctattgagtg ctaaaaatgt gctagtttac ctgaaagtac
     2041 ctgctagtaa agtgctagcg aagaacgaaa gtgtacccca cgggaaagcc ttgcactgca
     2101 aggcggggta ccttgtgtgc cccccagttc cccttatgct cttccgaaac acaggaagaa
     2161 attaagcgaa tttcaacagt aaaagcaaaa aataacagca gtgggattgg gggaacccca
     2221 aaaatttgaa cgactcctac cagtattttt ggtaggagtc gttcaaaata tcgcaacatg
     2281 gtaccatgtt gctccgcttg caaaaagaaa gcctacccca aaatggaagt aggctaatct
     2341 tgatattttt cgagtgttat cccaaatagt caacattttt ttataacaag caagggtagt
     2401 ttttattctt cttcgtctag agattgaccg tcaatcttca caatcccaca ggaaatatct
     2461 tcttctagcg taactttgta ggctaccgta tcaataagta gaatgtatga ccgacttaga
     2521 taattaaaca ttcttagcgt ttcgtctcga actacagtat ctaatcgtga taagtcaagt
     2581 ggctccaaat caagcaatag cgcttctagt tcactctcaa acttatctag tcgctccagt
     2641 acagcctgtg tgacttcgta ctcctctttt gtcatgttgt atcctgtcat taatcccata
     2701 agtaggacct ccaaaataat ttattttaga agtcctcacg caaatatgct aaaataatct
     2761 agcagtccgg aagattgcgt gaggaacttg ctaacttgct taatgtgagg gggcaagttc
     2821 tttttttatt ctccgtttac ccaatcgtgc atggttacgc ttggtttgtt ggttttcaga
     2881 ccagtaaacg taaattctag tttggcaatc ttattgcctt ttttggcttt gatttttgtt
     2941 acttctaaat tctcaaaata gttatggagt tctttcattg ctggttttaa aaccttttgg
     3001 tcaatgttac ccatttgata atattcgggt atgtctaaca attctctgaa gtcttcaata
     3061 ctgactacat aataccctgt tgaacgatat tgcattaaca aacgaaacaa tgttttagca
     3121 tatgttgagc gtattgaagt aaaagcggac agctcaaacc tactaaactc tgttgttaaa
     3181 ccatttatta tatagtctaa atcttgatta actcgaactt caacaaactt ttgttttttg
     3241 tcgattttaa atccattgaa taaaacaaaa tactctattt cgtcatcgtt ttctgtacga
     3301 taagaaaggt tcagcatatc tttatacaac ttttctaagt ctgctacaaa ggcttttgta
     3361 gcagtcattt tgtagtcact caattccttt aattcctcga aggagaaacg aatattctct
     3421 actcctttat ctttcattcg tgcgcataac gcaaagaaca gatccatttg ttttgcatta
     3481 aagtttttaa gtggcacaag atttaactca ttttgatatt taacagctaa ttccgattta
     3541 tcactcattc ctttcgcccc ctcacaattc gaatattaaa acaaacaacc aactttgtca
     3601 acagttggtt gtttgtttta atattatgaa aataaaacag ataaaaagtt gttggtagtc
     3661 agataaaaag ttgttggtag tcagataaaa agttgttggt agtcagataa aaagttgttg
     3721 gtagtcagat aaaaagttgt tggtaccatc tgctcttcct tagagccacg tggcacacag
     3781 cccgcctaaa aaggttttaa aaatatatat aaaatatata taaaagaaac gcgtgcgcgc
     3841 gcggaaattt cccgcccaaa attaacctct tttttctaca aacaggtccg cgatatttac
     3901 aaaactggca ctaaaaaatc aaaaaaggag tgagcgaaaa tcccgctcac attttgcttg
     3961 cagcgttcac ttcgttcact cgctaaaatc aaaatcaata ccgttgacaa ggtaaatcat
     4021 ttggttaggc tctatttgct tgtctgaaga tctttaagac atgtttagtt gttttagggt
     4081 atcttggtta ggattgcgcc ttgcaggcgg tcagggcaag ccctaacaac ccccacaaga
     4141 aattcttctt gtgatatact tgttttggca actgccccga aagggggtgt aaacatgatt
     4201 gaaagcttaa tttcgttgat tatcgcccca ctttttgtgg gtttagtact tttgctagtt
     4261 gatcgttggc tagacgatta gcagataatc tatgcagttg cttttctagc tccaacgctg
     4321 aaatcgcaac agtgtaaaaa agccctccta ctgagaatag gagggctttt tggtgtaaac
     4381 atgattttgc ttaatttcgc ttcgcactta cacaataaca tttttttttc tgtattttca
     4441 aacatcagat tagattacgc aaaacgtcaa ttagagaaat accatatcga aatagttagt
     4501 attaaacata gtaatactat gaggatagac aatattttac tttttggact atcttcaaag
     4561 cttttatttt ctggtctgta agtgggattc actactttcg cgttgtaatc cattgtactt
     4621 cccaaatata cttctgaagg aattaaaaaa aaataataaa attccgtaaa aaataattat
     4681 caatatgggc aaatcaatat taactaaaaa agaagttata tagcttaaaa ctaaaggaat
     4741 agtaatataa aatatatttt tattagatat ttttcatgaa ataacctcaa atttagcaac
     4801 tattttgtcc atcttgtatg cattgggata caaaatccac cggctttatg atatcctcct
     4861 gcagtccacc cgcctttttt ccaataaaat cctggctgac actctataga atcccactct
     4921 ctagcacttg cttctgtagt tgaacttaaa gcaacagaga taaaagacac ccctaaaatt
     4981 agaactaaca actttttcat aatgcttacc tcactttttt ttaattttta ttaatatatt
     5041 ttaatttaac tgtttttatg tgtcaataat ttatcaatta aattattttt aacgagataa
     5101 acctcgtcct tttgacatat ctcttgaata ttttttactc tgtt
//
