LOCUS       CP060658                 464 bp    DNA     circular BCT 31-AUG-2020
DEFINITION  Citrobacter freundii strain MGH283 plasmid unnamed4, complete
            sequence.
ACCESSION   CP060658
VERSION     CP060658.1
DBLINK      BioProject: PRJNA271899
            BioSample: SAMN08148336
KEYWORDS    .
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Earl,A.M., Walker,B.W., Manson,A.L., B hattacharyya,R.,
            Brennan-Krohn,T., Cosimi,L., Delaney,M., Ferraro,M.J., Gilmore,M.,
            Gussin,G., Hooper,D., Huang,S., Kim,D., Kirby,J., Onderdonk,A.,
            Peterson,E., Pierce,V., Shenoy,E., Wurster,J., Shea,T.D., Howe,W.,
            Livny,J., Cuomo,C.A., Neafsey,D.E. and Birren,B.W.
  TITLE     Multi-genus dynamics of carbapenem resistance within hospitals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 464)
  AUTHORS   Earl,A.M., Walker,B.W., Manson,A.L., B hattacharyya,R.,
            Brennan-Krohn,T., Cosimi,L., Delaney,M., Ferraro,M.J., Gilmore,M.,
            Gussin,G., Hooper,D., Huang,S., Kim,D., Kirby,J., Onderdonk,A.,
            Peterson,E., Pierce,V., Shenoy,E., Wurster,J., Shea,T.D., Howe,W.,
            Livny,J., Cuomo,C.A., Neafsey,D.E. and Birren,B.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2020) Infectious Disease and Microbiome Program,
            Broad Institute, 415 Main Street, Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.3
            Assembly Name                     :: MGH283
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 195x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/25/2020 14:20:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.12
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,097
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,921
            CDSs (with protein)               :: 4,921
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 21 of 58
            Pseudo Genes (incomplete)         :: 33 of 58
            Pseudo Genes (internal stop)      :: 15 of 58
            Pseudo Genes (multiple problems)  :: 9 of 58
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="MGH283"
                     /host="Homo sapiens"
                     /db_xref="taxon:546"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA: MA, Boston"
                     /collected_by="Massachusetts General Hospital"
     gene            62..138
                     /locus_tag="CXM82_25480"
     tRNA            62..138
                     /locus_tag="CXM82_25480"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:96..98,aa:Ile,seq:gat)
                     /name="tRNA-Ile"
     gene            248..323
                     /locus_tag="CXM82_25485"
     tRNA            248..323
                     /locus_tag="CXM82_25485"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:281..283,aa:Ala,seq:tgc)
                     /name="tRNA-Ala"
ORIGIN
        1 acgttctttg tagtgctcac acagattgtc tgatgaaaat tagcagtaag aaaatctctg
       61 caggcttgta gctcaggtgg ttagagcgca cccctgataa gggtgaggtc ggtggttcaa
      121 gtccactcag gcctaccaaa ttcgcctccg tgctgtgtta cggcgatgct cgcatacttc
      181 agtatgcttc gcaccaccgc gccttgcccg gaaacgaatt agggtacaga gattagcatt
      241 ctacgatggg gttatagctc agctgggaga gcgcctgcct tgcacgcagg aggtctgcgg
      301 ttcgatcccg catagctcca ccatctctta ctgcaaaaac acaagaaaac ttcagagtgt
      361 acctgaaaag gttcactgcg aagttttgct ctttaaaaat ctggatcaag ctgaaaattg
      421 aaacgacaca ctgtttcatt tctccgtaat aagaaatgga aaat
//
