LOCUS       CP060657                3076 bp    DNA     circular BCT 31-AUG-2020
DEFINITION  Citrobacter freundii strain MGH283 plasmid unnamed3, complete
            sequence.
ACCESSION   CP060657
VERSION     CP060657.1
DBLINK      BioProject: PRJNA271899
            BioSample: SAMN08148336
KEYWORDS    .
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Earl,A.M., Walker,B.W., Manson,A.L., B hattacharyya,R.,
            Brennan-Krohn,T., Cosimi,L., Delaney,M., Ferraro,M.J., Gilmore,M.,
            Gussin,G., Hooper,D., Huang,S., Kim,D., Kirby,J., Onderdonk,A.,
            Peterson,E., Pierce,V., Shenoy,E., Wurster,J., Shea,T.D., Howe,W.,
            Livny,J., Cuomo,C.A., Neafsey,D.E. and Birren,B.W.
  TITLE     Multi-genus dynamics of carbapenem resistance within hospitals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Earl,A.M., Walker,B.W., Manson,A.L., B hattacharyya,R.,
            Brennan-Krohn,T., Cosimi,L., Delaney,M., Ferraro,M.J., Gilmore,M.,
            Gussin,G., Hooper,D., Huang,S., Kim,D., Kirby,J., Onderdonk,A.,
            Peterson,E., Pierce,V., Shenoy,E., Wurster,J., Shea,T.D., Howe,W.,
            Livny,J., Cuomo,C.A., Neafsey,D.E. and Birren,B.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2020) Infectious Disease and Microbiome Program,
            Broad Institute, 415 Main Street, Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.3
            Assembly Name                     :: MGH283
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 195x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/25/2020 14:20:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.12
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,097
            CDSs (total)                      :: 4,979
            Genes (coding)                    :: 4,921
            CDSs (with protein)               :: 4,921
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 21 of 58
            Pseudo Genes (incomplete)         :: 33 of 58
            Pseudo Genes (internal stop)      :: 15 of 58
            Pseudo Genes (multiple problems)  :: 9 of 58
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="MGH283"
                     /host="Homo sapiens"
                     /db_xref="taxon:546"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA: MA, Boston"
                     /collected_by="Massachusetts General Hospital"
     gene            complement(90..3017)
                     /locus_tag="CXM82_25475"
     rRNA            complement(90..3017)
                     /locus_tag="CXM82_25475"
                     /product="23S ribosomal RNA"
                     /name="23S ribosomal RNA"
ORIGIN
        1 gcaaattctg tttatcaaca cacctcctgg gcatgtcgat gtaatctgta atcaaactga
       61 atcttgtgtc tgtctcttcg ccaaaacatc ttcggcgttg taaggttaag cctcacggtt
      121 cattagtatc ggttagctca atgtatcgct acacttacac acccgaccta tcaacgtcgt
      181 cgtcttcaac gttccttcag gacccttaaa gggtcaggga gaactcatct cggggcaagt
      241 ttcgtgctta gatgctttca gcacttatct cttccgcatt tagctaccgg gcaatgccat
      301 tggcatgaca acccgaacac cagtgatgcg tccactccgg tcctctcgta ctaggagcag
      361 cccccctcaa ttctccagcg cccacggcag atagggaccg aactgtctca cgacgttcta
      421 aacccagctc gcgtaccact ttaaatggcg aacagccata cccttgggac ctacttcagc
      481 cccaggatgt gatgagccga catcgaggtg ccaaacaccg ccgtcgatat gaactcttgg
      541 gcggtatcag cctgttatcc ccggagtacc ttttatccgt tgagcgatgg cccttccatt
      601 cagaaccacc ggatcactaa gacctgcttt cgcacctgct cgagccgtca ctctcgcagt
      661 caagctagct tatgcctttg cactaacctc ctgatgtccg accaggatta gctaaccttc
      721 gtgctcctcc gttactcttt aggaggagac cgccccagtc aaactaccca ccagacactg
      781 tccgcaaccc ggattacggg tctacgttag aacaccagcc attaaagggt ggtatttcaa
      841 ggatggctcc atgcagactg gcgtccacac ttcaaagcct cccacctatc ctacacatca
      901 aggaccagtg ttcagtgtca agctatagta aaggttcacg gggtctttcc gtcttgccgc
      961 gggtacactg catcttcaca gcgagttcaa tttcactgag tctcgggtgg agacagcctg
     1021 gccatcatta cgccattcgt gcaggtcgga acttacccga caaggaattt cgctacctta
     1081 ggaccgttat agttacggcc gccgtttacc ggggcttcga tcaagagctt ctccttacgg
     1141 ataaccccat caattaacct tccggcaccg ggcaggcgtc acaccgtata cgtccacttt
     1201 cgtgtttgca cagtgctgtg tttttaataa acagttgcag ccagctggta tcttcgactg
     1261 atttcagctc catgagtaaa tcacttcacc tacatatcag cgtgccttct cccgaagtta
     1321 cggcaccatt ttgcctagtt ccttcacccg agttctctca agcgccttgg tattctctac
     1381 ctgaccacct gtgtcggttt ggggtacgat ttcgtgttac ctgatgctta gaggcttttc
     1441 ctggaagcag ggcatttgtt acttcagcac cgtagtgcct cgtcatcaca cctcagcgtt
     1501 aaaaggtacc ggatttacct ggaacctccg cctacatgct taaaccggga caaccgtcgc
     1561 ccggctaaca tagccttctc cgtcccccct tcgcagtaac accaagtaca ggaatattaa
     1621 cctgtttccc atcgactacg cctttcggcc tcgccttagg ggtcgactca ccctgccccg
     1681 attaacgttg gacaggaacc cttggtcttc cggcgtgcgg gtttttcacc cgcattatcg
     1741 ttacttatgt cagcattcgc acttctgata cctccagcat acctcacgat acaccttcaa
     1801 cggcttacag aacgctcccc tacccaacaa cacatagtgt cgctgccgca gcttcggtgc
     1861 atggtttagc cccgttacat cttccgcgca ggccgactcg accagtgagc tattacgctt
     1921 tctttaaatg atggctgctt ctaagccaac atcctggctg tctgtgcctt cccacatcgt
     1981 ttcccactta accatgactt tgggacctta gctggcggtc tgggttgttt ccctcttcac
     2041 gacggacgtt agcacccgcc gtgtgtctcc cgtgataaca ttcttcggta ttcgcagttt
     2101 gcatcgggtt ggtaagtcgg gatgaccccc tagccgaaac agtgctctac ccccgaagat
     2161 gaattcacga ggcgctacct aaatagcttt cggggagaac cagctatctc ccggtttgat
     2221 tggcctttca cccccagcca caagtcatcc gctaattttt caacattagt cggttcggtc
     2281 ctccagttag tgttacccaa ccttcaacct gcccatggct agatcaccgg gtttcgggtc
     2341 tataccctgc aacttaacgc ccagttaaga ctcggtttcc cttcggctcc cctattcggt
     2401 taaccttgct acagaatata agtcgctgac ccattataca aaaggtacgc agtcacaccc
     2461 caaagggtgc tcccactgct tgtacgtaca cggtttcagg ttctttttca ctcccctcgc
     2521 cggggttctt ttcgcctttc cctcacggta ctggttcact atcggtcagt caggagtatt
     2581 tagccttgga ggatggtccc cccatattca gacaggatac cacgtgtccc gccctactct
     2641 tcgagttcac aacacatgca cttttgtgta cgggactttc accctgtatc gtgcgacttt
     2701 ccagacgctt ccactaacac acatgctgat tcagactctg ggctgctccc cgttcgctcg
     2761 ccgctactgg gggaatctcg gttgatttct tttcctcggg gtacttagat gtttcagttc
     2821 ccccggttcg cttcattacg ctatgtattc acataatgat agtgtgtcaa aacacactgg
     2881 gtttccccat tcggaaatcg ccggttataa cggttcatat caccttaccg acgcttatcg
     2941 cagattagca cgtccttcat cgcctctgac tgccagggca tccaccgtgt acgcttagtc
     3001 gcttaacctc acaacccgaa gatgtttctt tcgattcatc atcgacttgc aaaaatttga
     3061 gagactcgaa cacacc
//
