LOCUS       CP058802                9730 bp    DNA     circular BCT 15-JUL-2020
DEFINITION  Klebsiella pneumoniae strain BA27935 plasmid pColRNAI, complete
            sequence.
ACCESSION   CP058802
VERSION     CP058802.1
DBLINK      BioProject: PRJNA613369
            BioSample: SAMN14402329
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9730)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K. and Jacob,J.
  TITLE     Klebsiella pneumoniae hybrid assembly VBA27935
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9730)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K. and Jacob,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2020) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
REFERENCE   3  (bases 1 to 9730)
  AUTHORS   Veeraraghavan,B., Shankar,C., Vasudevan,K. and Jacob,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUL-2020) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Unicycler v. 4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 106.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 07/09/2020 11:57:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.12
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,707
            CDSs (total)                      :: 5,585
            Genes (coding)                    :: 5,448
            CDSs (with protein)               :: 5,448
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 58 of 137
            Pseudo Genes (incomplete)         :: 72 of 137
            Pseudo Genes (internal stop)      :: 27 of 137
            Pseudo Genes (multiple problems)  :: 19 of 137
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from Department of Clinical
            Microbiology, Christian Medical College, Vellore, India.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9730
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="BA27935"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pColRNAI"
                     /geo_loc_name="India"
                     /lat_lon="12.9246 N 79.1348 E"
                     /collection_date="2019"
                     /collected_by="Dept of Microbiology, CMC Vellore"
     gene            join(9406..9730,1..206)
                     /locus_tag="HBJ32_028430"
     CDS             join(9406..9730,1..206)
                     /locus_tag="HBJ32_028430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QLG19043.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERDKPAGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQISAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(234..413)
                     /locus_tag="HBJ32_028435"
     CDS             complement(234..413)
                     /locus_tag="HBJ32_028435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QLG19044.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGKE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            482..859
                     /locus_tag="HBJ32_028440"
     CDS             482..859
                     /locus_tag="HBJ32_028440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QLG19045.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQIETHERDGLRLSDETEKMASDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTSRRWWQWGKR"
                     /name="entry exclusion protein 1"
     gene            complement(921..1220)
                     /locus_tag="HBJ32_028445"
     CDS             complement(921..1220)
                     /locus_tag="HBJ32_028445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="QLG19051.1"
                     /translation="MPYEVEWKQGAIEDVTALFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1210..1473)
                     /locus_tag="HBJ32_028450"
     CDS             complement(1210..1473)
                     /locus_tag="HBJ32_028450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="QLG19046.1"
                     /translation="MSNSTHYENANFLRELAERLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            1850..2935
                     /locus_tag="HBJ32_028455"
     CDS             1850..2935
                     /locus_tag="HBJ32_028455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="QLG19047.1"
                     /translation="MAITIKSAVNVRSRVLIYSLLLFTGSAYAADIAESTFLTRDIGNG
                     LYEMSFDGKNNSLWAASSSSFDRDKGHGIVYQLEPSTLKTKSAVKIARLGFATALDEEH
                     ERLYIGNTLDGSVTVIDTLTGKELSVIPLTEGQDEAHFRPTREMVIDKKNQRLYVSSAA
                     DKGALWVIDMAVGKKIKTIDGLGHAVTGIALNKNGDNIYLVNGDGEIINLDASSYEIKK
                     RFVVEPEKKHFFLNVSLDEKNGRAFITDPDIPGVLVVDVNSGKILHRIDVINSLAILFN
                     PLRNEIYITHRNAKQVSIVDSNSYYVKTTIKTRLMPNSLSLSHDGENLYVSVKQEKKEM
                     STMKDYILKIELSKIAMEPEE"
                     /name="YncE family protein"
     gene            2932..5121
                     /locus_tag="HBJ32_028460"
     CDS             2932..5121
                     /locus_tag="HBJ32_028460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="QLG19048.1"
                     /translation="MNKYLGLSIVYMMYTSSVMADDSGAVSKDKNISPEETLIVHGTAV
                     SESTTEGTDSYTTQSANTASKMNLSFRDTPQSTTVITRRRMDDVAAKNIIDVVKTAPGL
                     YVSTAEGSGRPNFMSRGFNANVMYEGFSLPWNSYIPSSQTSSAIFDRVEIVRGATGLVQ
                     GAGDPSAAINMIHKRPTRQYQASTNIGAGRWNDYRGGVDVSGPLNDSATLRGRAVVDGE
                     NSETFRDSEKRRNNVIYGIMEADLTDNTLLTIGGYQQYEYNNHWWYDLPISGTGHHLNL
                     PRSTFTGNDWEFERSKSKTAFATLEQKFSNSWKLKFSTLQNWRNADLLGTATYRHKDTR
                     QSMYQQVWGSLREYNSKSYDLSLNGDYALFGRVHQFVAGTTQQKYTDKDTGYTYSPIYI
                     ENVNIFDHGSHSSKKPMKMSGDASYDVTEQKSAYAATHFLLSDNWKILLGGRLDWYEYT
                     SKGTSINTGYKVTRNITKYAGSTYNLDDHHTIYGSYTDIFNPQSSTDLNGKVIKPIVGK
                     NYETGIKGEYFNGLLNASLAVFQVDQENRARLLPDQTGCVTNGNPQCYEASGKVRTKGI
                     DFELQGMVTPLWEIGVGYTYADTRYIKDGDRSNEGKRFNTAVSPQNLFKISSMYTFDGK
                     LTNWRAGVNTYWQSKIYANITDTRDGNPVINKQKAYSTTDIIIGYTPMPQLDLQLNITN
                     VFDKVYYTSIGESIGYGTETYGEPRNVKISATYRF"
                     /name="TonB-dependent siderophore receptor"
     gene            6707..8668
                     /locus_tag="HBJ32_028465"
     CDS             6707..8668
                     /locus_tag="HBJ32_028465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="QLG19049.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIVWCAGGLV
                     AGILLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMGTNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEY
                     QPEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            8668..9399
                     /locus_tag="HBJ32_028470"
     CDS             8668..9399
                     /locus_tag="HBJ32_028470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="QLG19050.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGASGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     Typing          complement(5327..5907)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.625"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8669..9396
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.885"
                     /coverage="100.0"
                     /evalue="3.84e-144"
ORIGIN
        1 aggtggttga tgccgggaag tgctcaccgg agcagattag cgcgatgatt gaggtggtca
       61 gcgagcggca cggggaaagc gcggtgaacg agaagctgaa agcggagctg atgcgggaat
      121 ataacagccg ctattttaac gtgccggtgc ctgaactgcc ggaggacaaa cccgcccgcc
      181 ggtggtggcc gttcggcaaa aaataaacgg cagcaccggc catgccggtg ctgttattcc
      241 ttcccgagct tatcccgcat cacctgccag agtgtttccg catcctcgtg cagccgctcg
      301 cacatgtcgg ccggggtatc catatccagc tcgttcagtt tctcgagtaa cagcagcgac
      361 tggtccttaa taaatttagc catattgagg gcagtttttt cctgcttagt catgttttct
      421 gtccttccgg gtgtggtgtg cttcctgtcg ggtatactgt ctcaaaaccg gaggtgagac
      481 aatggcgtgg gtgacggtga gacaggcgac ggaactgacg gggaaagccc ggagttccct
      541 gtatagggat atggcgaaag gccgcgtatc ataccgtact gaagctgatg gtggtcgggt
      601 ggtggatacc agcgaactta tccgggtgta cggtgaatta agacagattg agactcacga
      661 gcgggacggg ttgagactgt cggatgagac tgaaaaaatg gcctccgacg cgttgatcgc
      721 ggagataaaa gcactcaggg aggaggtggc cggactgcgg caggaaatgc aggccatgag
      781 actgctggaa cacaaaccag agacagcaga gacaccggcc ggaacatcgc gccgatggtg
      841 gcagtgggga aaacgctgac gcgctgtgct tgtccaaccg gcgacggcca cgcaatgaat
      901 tgcgccccca tcaccggtta tcagcgaacg gtatggggat tctggcgctg gccaaccacg
      961 gccagaaccc ggactttcct ggtctggtca tcaacatcat agaggacatg ctgtcccatc
     1021 agactgatcc ggcgaacgtc ctcgccatag cgtgagtcca cttcatatag gcgcgggaag
     1081 tcggccagtt tgtccgctgc agcgagaact cgatcggtaa cgttctgcgc atcccacagg
     1141 gatgaatttt ccgcaatgta gtcaaacaat gctgtcacat cctcgatggc tccctgcttc
     1201 cattccacct catacggcat cgcgtagttc ctgatagcgg ccatgcagtc tctggcggag
     1261 ttgttccgtg gtcatgcccg ggcgcgtgtc agcgcgggcg gcggtcactt tagcgcgaac
     1321 ctgatcttca tattctgcct gggccagttc ttcgttagcc aggcgctgca acagtgcagc
     1381 tttgtccggg ccaccttcag gcagtatgcg gggcagtctc tcggccagtt cccgcaggaa
     1441 attggcgttt tcatagtgtg tactgttgct cattgtggag cctcccgggc tgatacgtcg
     1501 atatgaagga gtttagcagg gggcggtctg aaagaatatg ctcgtgtcag tcaggctgat
     1561 gaagacgaaa gctggtaaaa aagggcttac gacacccatt ttacgtcaaa ctgcacgaca
     1621 tgcagttgcg tgcatagcca tgcagcgggt taaaatttat caggcgcgtt tccggcggtt
     1681 ttccgggtgg tttgttgccg gttttatccc gtagccgccg gaaatgccct cagaccgtct
     1741 gagcggtgcg cgtaatgcga cgttatggta aatcatgtga ttaatggtag tgataatcat
     1801 tatcactttt gctatattct tgtttctttt attgtaagga aatgtaagta tggcaatcac
     1861 gattaaatcc gccgttaatg tccgtagcag agtattaata tactctttac tactgtttac
     1921 tggttcagcg tatgcggctg atatagctga atcaacattc ctgactcgtg atatcggcaa
     1981 tggtctgtat gaaatgtctt ttgatggcaa aaataatagt ctctgggcag catcatcgtc
     2041 ttcattcgac agagataagg gccacggaat tgtttatcag ttagaaccat ccactctgaa
     2101 aacaaaatct gccgttaaaa tcgcacgtct tgggtttgcc acagcactgg atgaggaaca
     2161 tgagcgactt tatatcggca atactctgga cggctcagtg acggtcattg atactctaac
     2221 cggaaaagaa ctttcagtaa ttccgttaac tgagggacag gatgaggctc atttcagacc
     2281 gaccagagaa atggtcattg acaaaaaaaa tcagcgtctt tatgtctcca gcgccgcaga
     2341 taaaggggct ctgtgggtta ttgacatggc tgtcgggaag aaaattaaaa ccattgatgg
     2401 actggggcac gctgttactg gaattgcgtt aaataaaaat ggggataata tttatctggt
     2461 taacggggat ggtgagatta ttaacctgga tgccagtagc tatgaaataa aaaaacggtt
     2521 tgttgtagag cctgagaaaa aacacttttt ccttaatgta agtctggatg aaaaaaacgg
     2581 acgtgcattt attaccgacc cggatattcc cggggtgctg gttgttgatg taaactcggg
     2641 aaaaatacta cacagaattg atgttataaa ttcactggcg attttattta atcctttacg
     2701 gaacgaaatc tatatcacgc accgtaatgc aaaacaggtc agcattgtgg acagtaattc
     2761 ttattacgta aaaacaacga taaaaacccg cctgatgcca aacagtctgt ctttatcaca
     2821 tgacggtgaa aatctgtacg ttagtgtaaa acaagaaaaa aaagagatga gtactatgaa
     2881 ggattacata ctcaaaatcg aattatctaa aattgctatg gagccagaag aatgaataaa
     2941 tatttagggc tgagcatagt atatatgatg tatacatcat ctgttatggc tgatgattct
     3001 ggcgcagtta gtaaagataa aaatatttca ccagaagaaa cattaattgt tcatgggacc
     3061 gctgtttctg aaagcacaac agaaggaaca gattcataca caacccagtc agccaataca
     3121 gcatcaaaga tgaatctgtc attccgggat acacctcagt caactacagt cattacgcga
     3181 cgaagaatgg atgatgtagc agcaaaaaat attattgatg tggttaaaac cgctcctgga
     3241 ttatatgtca gtacagcaga aggttccgga agaccgaatt ttatgtccag aggatttaat
     3301 gctaatgtca tgtatgaagg atttagcctg ccgtggaatt catacattcc aagttcacaa
     3361 actagttcag ctatttttga ccgggtggag atagtccgcg gggcaacagg tcttgttcag
     3421 ggtgccggag acccttcagc cgcgataaat atgatacaca agcgtccgac ccggcaatat
     3481 caggccagca ctaacatcgg tgccggacgg tggaatgact accggggtgg ggttgatgta
     3541 tcaggaccac tgaatgacag cgcgacctta cgcggaaggg cagttgtgga tggcgaaaac
     3601 agtgagacct tcagggatag tgagaaacgc aggaataacg tcatttacgg aattatggag
     3661 gcagatctga ctgataatac cctacttacg attgggggat atcagcagta tgaatataac
     3721 aatcattggt ggtatgactt acctatttct ggtaccggac accaccttaa tcttcctcgc
     3781 tcgacgttta caggcaatga ctgggagttt gaaagaagta aaagtaaaac tgcatttgca
     3841 actcttgagc agaagttcag taatagctgg aagctgaaat tttcaacgct gcaaaactgg
     3901 agaaatgctg acctgttagg gacggcaaca taccggcata aagatacccg acaaagtatg
     3961 tatcagcaag tatggggatc actgagggaa tataattcca aaagctatga cctgtcgctt
     4021 aatggggact atgctttatt tggtcgtgtt catcaattcg tggctggaac aactcagcaa
     4081 aaatacaccg ataaagatac aggttataca tattccccaa tatatattga gaatgttaat
     4141 atatttgatc atggttcaca ttcttcgaaa aaacccatga agatgtcagg tgatgcatcc
     4201 tacgacgtca ctgaacaaaa aagcgcctac gcagcaactc attttctact tagtgataac
     4261 tggaaaatat tgctgggtgg ccgtcttgac tggtatgaat atacaagtaa aggtacatcc
     4321 attaatacag gttataaagt taccagaaat atcaccaaat atgcaggttc aacatacaat
     4381 cttgacgatc atcataccat ttatgggagc tataccgata tattcaatcc acagtcaagc
     4441 accgacctca atggtaaagt gatcaagcct attgttggta agaattatga gacaggtatt
     4501 aagggtgagt attttaacgg cttgcttaat gcctctctgg cggtatttca ggtagaccag
     4561 gaaaatcgag ccaggctgct tcccgatcag acagggtgtg ttactaatgg caatcctcaa
     4621 tgctatgagg ctagcggtaa agtacgcaca aaagggatag atttcgaact tcagggcatg
     4681 gttacgccac tctgggaaat tggggtggga tatacatatg cagatacgcg atatataaaa
     4741 gatggagatc gtagtaatga aggcaagcga ttcaatactg ccgtgtcgcc acaaaattta
     4801 tttaagattt cgagcatgta cacctttgac ggaaaattaa caaactggcg agcaggagta
     4861 aacacgtact ggcaaagtaa aatatatgct aacataacag acacaaggga tggcaatccg
     4921 gttattaata aacaaaaggc ttattcaaca actgacatta ttattggcta tactccaatg
     4981 ccacaactgg atttacagct caatataact aatgtctttg ataaggttta ttatacatcc
     5041 ataggtgaat cgattggata tggcacggaa acatatggag aaccaagaaa tgttaaaatt
     5101 agtgcaacct atagattcta accatgcgga ttgggtcttc tcctcggatt tgcccctata
     5161 aatccgtctt ttgtggttcc ggcgtacaac cccctgtttg ccacttcagt tgatggttaa
     5221 tcaaatcatg tagaggatct tcttgagatc ctccatggtt cgtcgtaatc ttttgccctg
     5281 taaacgaaaa aaccacctgg ggaggtggtt tgatcgaagg ttaagtcagt tggggaactg
     5341 cttaaccgtg gtaactggct ttcgcagagc acagcaacca aatctgtcct tccagtgtag
     5401 ccggactttg gcgcacactt caagagcaac cgcgtgttta gctaaacaaa tcctctgcga
     5461 actcccagtt accaatggct gctgccagtg gcgtttttgc gtgtctttcc gggttggact
     5521 caagtgaaca gttaccggat aaggcgcagc agtcgggctg aacggggagt tcttgcttac
     5581 agcccagctt ggagcgaacg acctacaccg agccgagata ccagtgtgtg agctatgaga
     5641 aagcgccaca cttcccgtaa gggagaaagg cggaacaggt atccggtaaa cggcagggtc
     5701 ggaacaggag agcgcaagag ggagcgaccc gccggaaacg gtggggatct ttaagtcctg
     5761 tcgggtttcg cccgtactgt cagattcatg gttgagcctc acggctccca cagatgcacc
     5821 gaaaaagcgt ctgtttatgt gaattctggc aggagggcga agcctatgga aaaacgccac
     5881 cggcgcggcc ctgccgtttt gcctcacatg ttagtcccct gcttatccac ggaatctgtg
     5941 ggtaactttg cgagagttag cagcgcccgc cgcagtctca cggccggagc gtagcgactg
     6001 agtgagggag gaggcgcaat atcccccctc ccgcagacgg ccaccggacg cgcggatgtg
     6061 acatattgca ccgtgttacc gcctttccct tccctgccga taaatcattc agataggtga
     6121 agtctgtcgg ttttctgctg aatattttac gttaacctat ctctgttgct tttttctcct
     6181 tgcggagaaa tgcaggtcat ccccgctggg gcgtggctga cttcaggagg aaatcccctg
     6241 cttacgctgg ctggacgcca ccgacgcacg gtctttcccc ctctttctgg ctcagctcgg
     6301 ctggacgccg gccacgccag aaattcaccc ggcccccgtc aggctcacgc ctgaccaccc
     6361 acaaaaaccc acggtgctga acgcacctga cgaagcagca aagctgctcc tgattttgcg
     6421 ggtggtcagc cgctcgctat ggcgaactct gacgggggcc ggaacaccgg aaaagaccgc
     6481 actggggtgc cgccttttcc tctcacacgc tggggcgtgc tcgttgttcc tccggggctg
     6541 cgccttttcc gcttggggcg gaaagaggct ggcctccgtg gtgaaagctg aatgcaatca
     6601 ccacggacgt gaccgccgcc gccatgcctc ggggcatgtc cgcggcttgt gcagcagggg
     6661 ctgcacacca cgccggtaaa cccgtcgtct gaaggagaga gattgcatgt ttaatacgga
     6721 ttcgcttgcc tggcagtggc atatcccgga gctggcccgg cagatttacc cgtgggtcag
     6781 ctggctgtac agggagtatt acggtctggc cggggctttc ctggttatcg gcctgttttt
     6841 cggtctggct gaactctggg ccggacggat gcgttttgtg gggctgagtc ataaggagcg
     6901 acgggagtat gccgatcgct ataagcgcga taataaccgg actgcgccat tttcggtgct
     6961 ggcttcgctg gattcgcccg ggcggatggt cagggcacgg cggcatgtct ggaactacgc
     7021 gtactggctg accggcgctg ccggtctggc ccttctgggg gggctggttg tcgccgtgtt
     7081 tatcgagagc tggggagaaa ccgtcctgcg tccggacctc tacgcgaacg cagattatta
     7141 cctgctgcag ccggctaacc tgttcgggat tgtctggtgt gccggagggc ttgtcgccgg
     7201 catccttctg gcgcggctgc tggcatttct gctgcttgac ggtcattttg cggccgctga
     7261 cgaggcggtg aatgcgaaac tgaaccagga gagccggcgc agcagccagc ggaccggaga
     7321 aatgaccgac gtccgccacc tgcatttcgg ggagccggta ccggttaacg cgctggccga
     7381 tttttccaca gagcaggccc ggaaacagca ggcggtgttt ctgggtaagg atgagcaggg
     7441 ccagccggtg ctggtcccgc gtgatacctg gcgcaagacc aatattcaga tcctcggcct
     7501 gcccgggagc ggcaagagcg tgatgggcac caacgcgctc attcgctgcg tacgcgattt
     7561 cggggacgcg gtggtgtact tcgaccccaa aggggacgcg tgggcgccgc acgtcttccg
     7621 ggcgcactgc ccggacttca ccctgctgga tctgcggccg gggaaaccgg cgcagctgaa
     7681 tctgtttcgc gatctcgacc agtacgccct gaagaatctg ctggttgccg ggtttaacct
     7741 gagtgagacc ggcgacgtgg cggaccacta ccgcatcagc gagcagaaag cggcgaagct
     7801 gattgcggag cagttcccgc agggggcaaa cattcagcag gtgctggcgg cggcatacgc
     7861 gctgccggag gccctgaaaa aggacgtgaa ggggctgatc accaagctgg agaacgtggc
     7921 cgacctgagc gtcctgcaga cggacagcgg catcgacgtg gcggggataa tcaacggcgg
     7981 cggctgcctg tacgtcatcg gctcgatgga cgatgaggcg gtgatccggg tgcagaagat
     8041 gctgtttgcc cgctgcgcgc aaatcatcat tgcgcgggat gagttccgga ggtggccgca
     8101 cgcgagcatc atgctcgatg agattaaata cctgctgtcg aagtacgtgc tgaacgcgct
     8161 ggggacgctg cgcagtcggg actgcaacct gctgctcgcg caccagtcgc tgggcgactt
     8221 cgggcagtgc ggccaggatc tgcccgctga gtttgtgaaa accacggtgc tggataacac
     8281 gccgatccgc tggttctacc gggcggccag ccaggagtcc gcgcagtggg cggcgggcca
     8341 gaccggggaa atccgggtgg acgtggagcg ccgccgggcc agccgggagg cggggaacgt
     8401 ggagcatatc agcggggaca gcttcattca gaaggaggcg cggccgctgt ttgacgtgaa
     8461 caccctgcag cacctgcctg acgggtttgc ggtgatgacc gggctgggcg tggcccggat
     8521 ggcgttcagc agcccgctgc gggttgaacg ccgggaaatc ccgctgcagc gcttcccggt
     8581 gctggcgaaa acggacccgc tggcggagta ccagccggag gccgggcggc agcggccggg
     8641 tgatgatgat tttgcggggc tgtactgatg gcactggagc gatacaacgt cagccacgcg
     8701 aagcggcagg cgcggaacgc ggagaaaacc cggctgaccc tgcgctggct gcgggaagag
     8761 ctgtgttcca cggcggagct ggttgcgcga cggctgggga ttgcggcggt gcagccggtt
     8821 taccgcttcc tggacagcct ggtggcgaaa gggctgctgg tccgggcgaa atacccggtt
     8881 gacggccgtc aggtcagcgt gtggggactg acgccgcacg gggtggcgtt ctcgttcgac
     8941 gaggacgaac cgctgacgga cgttattccg tttcagccgt caagggtgtc ggcggcgcag
     9001 ctgccgcacc ggctggccgt gcagtccctg cgtctcgcca tggaggcccg gggggcctcc
     9061 ggctggcggt acctgcaccg tatggccctg aaggggatga aggtgccgga cgcgctggcg
     9121 gagctggacg gcagaacggt ggcgtttgag gtggagcgca ccgtgaagag ccgcaggcgc
     9181 tatcaggagg tggtgtccgg gtatctgttc aatcgcaaag cgaacggcat cgatgaaatc
     9241 tggtacatct gcccggaccg ggcgacgcag gtcagggtgc agcgggcgat cctgtcggtg
     9301 gatgaaatcg tgaatccaca gaccggcgag gcccgcaaaa cggcggagct ggaccgggag
     9361 cggctgtttg cgtgctttaa atttatgaca acggagtgag ggattatggc gctgtacatg
     9421 gtatttattg aagacgcgga ggaccgggac ggcgagcgct gcatcatgcc gaccagcgag
     9481 catcttgaag atatggtggt gctcgcctgg cagaccgggg cgtatgtttt ctgttttgtc
     9541 gaccgggccc agcatctggc gatgatgaaa tccatcggga tgtatgacga gcggctcaac
     9601 agcgacgaaa aagccacgta ctttttcacc ggtaaaaatc cgcaatggga acgggacaaa
     9661 cccgcaggaa tgaagctgtg tgattacctc tatcgcaagg tggagcggtt cgccatcgag
     9721 aacggtgtgc
//
