LOCUS       CP058111                1306 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB01-C04 plasmid pRHB01-C04_8, complete
            sequence.
ACCESSION   CP058111
VERSION     CP058111.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15147948
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH11 T1-C04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 115.102049370518x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 14:38:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,859
            CDSs (total)                      :: 4,740
            Genes (coding)                    :: 4,643
            CDSs (with protein)               :: 4,643
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 47 of 97
            Pseudo Genes (incomplete)         :: 42 of 97
            Pseudo Genes (internal stop)      :: 23 of 97
            Pseudo Genes (multiple problems)  :: 15 of 97
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB01-C04"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB01-C04_8"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(415..951)
                     /locus_tag="HVV31_24295"
     CDS             complement(415..951)
                     /locus_tag="HVV31_24295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QMR37934.1"
                     /translation="MQTQNVITEKEREIRSRDLTENAKKLAHERREAEKNSNFTQVSPL
                     GWKRLMELAKGNQGAFGLYSFFAQHIDGSCGAVVADQSFLAAQFNVSTRTIIRWINYLE
                     ENRAVVRIPVAGKVCAYALNPNEVWKGYGNTKEYAAFITKTLVNKDGDIQRRIMAMFHG
                     NDARQGMEGDTPEAE"
                     /name="helix-turn-helix domain-containing protein"
ORIGIN
        1 attggggaag gtggttgtgt tcacgcagtg agcgcaacca cctgccagac gggttttagc
       61 gccgcaggcc gcacatgttt ctgagccaac ggcgaagaaa catataagtg cgcatctctc
      121 taatttcacg aatttgagag atgaaaccag tctggataat cggagtactc cgcctctagc
      181 cgtcctggtg aaccagtccg gcaagaccca cgaaatcacc cgtaaaacgc cctaaaacgc
      241 gctctgacgc gttttttgac gttctacggg ctcacgtggg ctttgggcgg aaaaatggct
      301 tacgtgcgtt ctgagagcgt tttagggctt tgcagggcgg agggggtgaa gcagctccct
      361 gtggaacctg taccgccctt gcgggcgggc ttcggcgatt tggcgaacgg gaatctattc
      421 ggcttctggc gtgtcgcctt ccatgccctg acgggcatca tttccgtgga acatggccat
      481 gattcggcgc tgaatgtcgc cgtctttgtt gactagggtt tttgttataa acgccgcata
      541 ttccttggta ttcccgtacc ccttccaaac ttcatttgga ttcaatgcgt atgcacaaac
      601 cttaccggcg acaggaatcc tcacaacagc cctgttttcc tctaaatagt tgatccatct
      661 aattattgtt cttgtcgata cattgaactg cgccgccaaa aatgactgat ccgccactac
      721 tgcgccacaa gatccatcga tatgctgagc aaaaaaagaa tacaagccaa aagctccctg
      781 atttcctttg gctaattcca ttaaacgctt ccagcctaat ggggacactt gtgtgaaatt
      841 tgagttcttt tctgcctctc gcctctcatg agccagtttt ttggcatttt cggttagatc
      901 cctagatcga atttctcgct ctttttcggt gattacattt tgagtttgca tctcaatctc
      961 catgtcattt caatgtgaca agacaagtca tactatacga cattttatgt cgtagtatat
     1021 gtcatttatg tcacatatta cgaaatgatg tgacatccta gggtgtcata acgttgtgac
     1081 atcctagggt gtcatatcag aaatgttaaa tgtcttttaa atcaatgaat tattaatttt
     1141 tgctattctt atctaaagag atctcacagc gcgctgcgag atctcctggt agcaccctcc
     1201 tttcagtcgg gcaaacggcg aaaatttaac cattggcaac cgaaaaatcg tgggggcccc
     1261 ttcaggggtt ccgatttttt tgttgccaac gcaccgatca aaaaaa
//
