LOCUS       CP058100                1306 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB01-C09 plasmid pRHB01-C09_8, complete
            sequence.
ACCESSION   CP058100
VERSION     CP058100.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15147953
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH11 T1-C09
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 90.9454302587345x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 14:43:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,860
            CDSs (total)                      :: 4,741
            Genes (coding)                    :: 4,643
            CDSs (with protein)               :: 4,643
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 48 of 98
            Pseudo Genes (incomplete)         :: 42 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 15 of 98
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB01-C09"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB01-C09_8"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            491..1027
                     /locus_tag="HVV36_24300"
     CDS             491..1027
                     /locus_tag="HVV36_24300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QMR28357.1"
                     /translation="MQTQNVITEKEREIRSRDLTENAKKLAHERREAEKNSNFTQVSPL
                     GWKRLMELAKGNQGAFGLYSFFAQHIDGSCGAVVADQSFLAAQFNVSTRTIIRWINYLE
                     ENRAVVRIPVAGKVCAYALNPNEVWKGYGNTKEYAAFITKTLVNKDGDIQRRIMAMFHG
                     NDARQGMEGDTPEAE"
                     /name="helix-turn-helix domain-containing protein"
ORIGIN
        1 aaattcgtga aattagagag atgcgcactt atatgtttct tcgccgttgg ctcagaaaca
       61 tgtgcggcct gcggcgctaa aacccgtctg gcaggtggtt gcgctcactg cgtgaacaca
      121 accaccttcc ccaatttttt ttgatcggtg cgttggcaac aaaaaaatcg gaacccctga
      181 aggggccccc acgatttttc ggttgccaat ggttaaattt tcgccgtttg cccgactgaa
      241 aggagggtgc taccaggaga tctcgcagcg cgctgtgaga tctctttaga taagaatagc
      301 aaaaattaat aattcattga tttaaaagac atttaacatt tctgatatga caccctagga
      361 tgtcacaacg ttatgacacc ctaggatgtc acatcatttc gtaatatgtg acataaatga
      421 catatactac gacataaaat gtcgtatagt atgacttgtc ttgtcacatt gaaatgacat
      481 ggagattgag atgcaaactc aaaatgtaat caccgaaaaa gagcgagaaa ttcgatctag
      541 ggatctaacc gaaaatgcca aaaaactggc tcatgagagg cgagaggcag aaaagaactc
      601 aaatttcaca caagtgtccc cattaggctg gaagcgttta atggaattag ccaaaggaaa
      661 tcagggagct tttggcttgt attctttttt tgctcagcat atcgatggat cttgtggcgc
      721 agtagtggcg gatcagtcat ttttggcggc gcagttcaat gtatcgacaa gaacaataat
      781 tagatggatc aactatttag aggaaaacag ggctgttgtg aggattcctg tcgccggtaa
      841 ggtttgtgca tacgcattga atccaaatga agtttggaag gggtacggga ataccaagga
      901 atatgcggcg tttataacaa aaaccctagt caacaaagac ggcgacattc agcgccgaat
      961 catggccatg ttccacggaa atgatgcccg tcagggcatg gaaggcgaca cgccagaagc
     1021 cgaatagatt cccgttcgcc aaatcgccga agcccgcccg caagggcggt acaggttcca
     1081 cagggagctg cttcaccccc tccgccctgc aaagccctaa aacgctctca gaacgcacgt
     1141 aagccatttt tccgcccaaa gcccacgtga gcccgtagaa cgtcaaaaaa cgcgtcagag
     1201 cgcgttttag ggcgttttac gggtgatttc gtgggtcttg ccggactggt tcaccaggac
     1261 ggctagaggc ggagtactcc gattatccag actggtttca tctctc
//
