LOCUS       CP058098                2590 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB01-C09 plasmid pRHB01-C09_6, complete
            sequence.
ACCESSION   CP058098
VERSION     CP058098.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15147953
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH11 T1-C09
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 90.9454302587345x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 14:43:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,860
            CDSs (total)                      :: 4,741
            Genes (coding)                    :: 4,643
            CDSs (with protein)               :: 4,643
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 48 of 98
            Pseudo Genes (incomplete)         :: 42 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 15 of 98
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB01-C09"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB01-C09_6"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(840..1829)
                     /locus_tag="HVV36_24285"
     CDS             complement(840..1829)
                     /locus_tag="HVV36_24285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020240588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="QMR28355.1"
                     /translation="MQNPKLQYLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYEHYGAR
                     MASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDARWMFLTLTVRNCAIGELGEMLNRMNAAFQRMKVRKEFLPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMAPPGMLNGKSYVRHERWVELWRECLRVDYDPNVDVRAVKPRKPKDG
                     ESLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGALKDVLK
                     LDQETDADMVIGDDISEGDDDGSRIAFEWKTEVRKYRRSPTKDKTESD"
                     /name="protein rep"
     Typing          complement(373..628)
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.574"
                     /coverage="89.0"
                     /evalue="2.65e-108"
     Typing          complement(754..837)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.941"
                     /coverage="93.0"
                     /evalue="1.22e-26"
     Typing          2402..2483
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.976"
                     /coverage="93.0"
                     /evalue="3.4000000000000004e-27"
     Typing          923..1114
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.446"
                     /coverage="100.0"
                     /evalue="2.86e-91"
ORIGIN
        1 tttgtcgatc acgcggaacg gctgcggcac aaaaacggca aaatgatttg ctgtccggtc
       61 gccagaattt ggcgcgtcgg tggcgatggc tgtacggaac gggaacgacc gcggggtggg
      121 gcgcgtggtg taaggacttg ttacgcgcac cgctaaaacg cgctgaacgc gattctgccg
      181 cgaacaccgc aaaaagggat gttttcccca gggtaaaccg gaaaaatgcg tcaggaacgt
      241 tttcagcgcg ttgcatgact atgcatgaaa ctgaatggcg atcggtttgg gcgcatacag
      301 tgcccatgag acatattttc ggacattttc aattcgtgaa tccctgactc attgctacat
      361 atctgaacag acacccctag cagcgccccc aggggcgtcc tgtaaaaaaa cacaccgcgc
      421 cgctagcagc acccctaata taaaattatg ttttttatga aaatagtcag taccacccct
      481 acaaagcggt gtcggcgcag ctacaacacc gcgccgacac cgctttttta aatatcacaa
      541 agagagcaag agaaactaat ttgtcataac tctctattta taaagaaaaa tcagcaaaaa
      601 attgtttttg cgtggggtgt gggcatttca ggcatgagaa cagactcgcg tttttccgga
      661 gtatcagcgg aattcttgat taagaaacac tcgacgcaga gcgaaatttt atgcaacagc
      721 ggctgtactg cacagggatt atttatcagt tgaaacagga caggatgtgt aattgtaata
      781 ccgtcacacg cgacgctatc acaattacca cctggtcagg gctgcgcccc gacaccctgc
      841 tagtctgatt cagttttatc tttcgtcggt gagcggcggt actttctgac ctcggttttc
      901 cattcgaacg cgatgcgtga accgtcatcg tcgccctcgg aaatatcatc tccgatcacc
      961 atgtcggcgt cggtttcctg atcgagtttc agcacatctt tgagtgcgcc gccggtcgcc
     1021 acaaagcggc gcttgtgcgt ctggcgagtc agttcgagga accattcagg atcggccacc
     1081 atgtcagccg gcttcgtgga atatttcagg gtttcggcca ctgcgccgcg caccagttca
     1141 gcggtcgcgc aggctaagga ctcgccatcc ttcggcttgc ggggtttcac tgcccggaca
     1201 tcaacattcg ggtcataatc aacccgcagg cactcccgcc atagctcaac ccagcgttca
     1261 tgcctgacgt aacttttccc atttagcatg ccaggaggag ccatcatcaa ggtatggaag
     1321 tgcgggtgtg cgctgccgtc gctgccgcgg gtaacttccg ttgtgcggat ccagccctga
     1381 acaggcagga actccttgcg aaccttcata cgctgaaaag ctgcattcat ccggttcagc
     1441 atctcgccca gttccccaat cgcacagttg cgcacggtca gggtcaggaa catccagcgg
     1501 gcatcgggat aatccgccac gatacgcggc agggactggt agaaacgggc ttgccacatc
     1561 agggagcgcc tccactggca gacaggacag tgccggacac ggcagaaatg tgcctcacgc
     1621 agcctcagac gcgtttctcc ggtttccttg agggtagacc acccgaaacg cagtaaaccg
     1681 ccacaggaag ccatacgagc cccgtagtgc tcatattcgg ccgcaagcag gtaaatccct
     1741 cccacgtcgt cagaaacgct tctgtgtgcg tcccagggcg catcagaggg ggaataatca
     1801 gtgaggtact ggagtttagg gttttgcatg cacaccgccc catactgggc gatggctcga
     1861 ttgacgtgtt atcattgcca tatcacctgt tgctctcatc atgaatccag tttctgcagg
     1921 tggtcacacc cccgaacgat gcccgtcgtt gcgggggtca cttttaacgc aaccactccc
     1981 gacagaataa ctccaaaacc attttccggc aaatcccccc tcaggccttg cggcacaagg
     2041 gctggaggcg aatttgaatc ctagagttat cccttaagta taaatacaag gatcacgcgc
     2101 gagatttgct tgaattttga gctggatttt cagtaaccaa aaacctccca accccaccca
     2161 cttacaccca tctcatccac agactttcac cccgattttg tgaacaacaa aaaaccgtct
     2221 tgcaggtggt tggcctcact ccgtgacacc aaccaccatc cccagtgttt tccccgtgcc
     2281 gtcgtcaatc acaaaatcgg aacccacgcc agaggcgcgg ggccccgcga ttttctgttt
     2341 gacgagttcg ggaacggtga gcggtacgcg gaattttttc gcgggccgtg aggcgttggc
     2401 ggggtgtcgg ggcgcagccc tgaccaggtg gtaattgtga tagcgtcgcg tgtgacggta
     2461 ttacaattac acatcctgtc ccgaaaattc ccgccgtttc tcggtttttt gagcgtcgca
     2521 gaccgcacga caaacgaaac gaagtgaagt ttgtataagt gcgcacttcg tgagattccc
     2581 tgactgccag
//
