LOCUS       CP058043                8136 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB03-C06 plasmid pRHB03-C06_4, complete
            sequence.
ACCESSION   CP058043
VERSION     CP058043.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15147976
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8136)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 8136)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH01 T1-C06
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 115.344480386831x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 13:29:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,382
            CDSs (total)                      :: 4,264
            Genes (coding)                    :: 4,002
            CDSs (with protein)               :: 4,002
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 262
            CDSs (without protein)            :: 262
            Pseudo Genes (ambiguous residues) :: 0 of 262
            Pseudo Genes (frameshifted)       :: 119 of 262
            Pseudo Genes (incomplete)         :: 122 of 262
            Pseudo Genes (internal stop)      :: 45 of 262
            Pseudo Genes (multiple problems)  :: 22 of 262
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB03-C06"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB03-C06_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(287..436)
                     /locus_tag="HVV59_21850"
     CDS             complement(287..436)
                     /locus_tag="HVV59_21850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMQ60531.1"
                     /translation="MPSEHDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            1521..1838
                     /locus_tag="HVV59_21855"
     CDS             1521..1838
                     /locus_tag="HVV59_21855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMQ60532.1"
                     /translation="MKKIFILSAALIMAGCATKQYPQAVNVTGPESSAMDCKAVALEIA
                     KTRSVQQEIEETGEFDGRTVLGFLGDFGIGNGMAKGEARKKAANRLNQLEALKAAKCAD
                     Q"
                     /name="hypothetical protein"
     gene            2459..2743
                     /locus_tag="HVV59_21860"
     CDS             2459..2743
                     /locus_tag="HVV59_21860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QMQ60533.1"
                     /translation="MSFFDELKASLEEAVEIKNGTKAPARVTRYELADVRAIRENLNIT
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFDELAAH"
                     /name="helix-turn-helix domain-containing protein"
     gene            2942..3407
                     /locus_tag="HVV59_21865"
                     /pseudo=""
     CDS             2942..3407
                     /locus_tag="HVV59_21865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517860.1"
                     /note="frameshifted; incomplete; partial on complete
                     genome; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            3379..3646
                     /locus_tag="HVV59_21870"
                     /pseudo=""
     CDS             3379..3646
                     /locus_tag="HVV59_21870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558268.1"
                     /note="frameshifted; internal stop; incomplete; partial on
                     complete genome; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(3739..5193)
                     /locus_tag="HVV59_21875"
     CDS             complement(3739..5193)
                     /locus_tag="HVV59_21875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="QMQ60536.1"
                     /translation="MTALVSSFKWVGIGQLVKIATQLVSLFYLTHLIPPSSYGLLAMSS
                     VVMNLASIFNDMGTGAAVIQRQEASIGFYNYIYRINIFTGLFVMLLVIITSPLIVVYFN
                     TPELYPVLFLLAFSFPISSLTITHKAKLEKEMNFKVIIAIEIFSSLTGMVLAIVLANLG
                     FGVYSLIFQTLTTISISTICFLKVSGLSLSLRIKNISHSDKSGVIGFSGYLLSFNLINY
                     FSRNLDSILIGRFFSSAILGVYSIAYRIMLFPLQSLTLVFSRVFLPHLSKNINNTNNTN
                     KQDYLNALKLILSLSAPLMLGLSATSFSFTRIFFDPKWHLLGDLLHWLAPTAIIQSALS
                     TTGSVFMAYGKTRWLFFLGCIGAFLMGLSFIIGVHFDIKTLVYLYFFANLINSLPAFIL
                     LKNILKFSYIELVNVFFRTISPAFLMFLLIKVWQHFSFFKNASIDFSFNVALGIFSYIV
                     FYTLFNFNEVVRTGVIHKLRCMTKLK"
                     /name="lipopolysaccharide biosynthesis protein"
     gene            5442..6145
                     /locus_tag="HVV59_21880"
                     /pseudo=""
     CDS             5442..6145
                     /locus_tag="HVV59_21880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            complement(6604..7587)
                     /locus_tag="HVV59_21885"
     CDS             complement(6604..7587)
                     /locus_tag="HVV59_21885"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="QMQ60534.1"
                     /translation="MKKKFSIIVPVYNVEKYLRECIQSVLDQDFNDYEIICVNDGSTDG
                     SLDIILSFDDQRIKVINQENQGQSVARNVAIESSTGEYIWFLDGDDKISHGSLKLIDKI
                     TNEKNYGIIFFNAHVFFDTPDLKKKGWHDYKRSTFGEIDALTFFEKEMANKKLIVSPCC
                     YVFKRELLNGNAFIPHIYHEDNHFIATLIFDNYSTICLSLDEELFLRRVRNDSVMTSKK
                     SMRHIIGYSTCIDELTKYKKIKGIQNKSFDRFVNGMVLSLFSISETIKTKEAMNYVARY
                     KKHPGLSFKDSVSINYPLIKKTYLKLKNGKVFNYLRNGIINKKSNP"
                     /name="glycosyltransferase family 2 protein"
     gene            complement(7672..8040)
                     /locus_tag="HVV59_21890"
     CDS             complement(7672..8040)
                     /locus_tag="HVV59_21890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMQ60535.1"
                     /translation="MANFNPGPRTAIAAVRDSKNDIETWKKNGIRREEIVTVLNERYGL
                     NISLVAFDKALYRLRKSTDEPSLADVDSVSAAETNFSEASGPDNGFFLSGNDFMRAEEE
                     SQRVVESFRDSLSRKKGF"
                     /name="hypothetical protein"
     Typing          complement(472..645)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.571"
                     /coverage="100.0"
                     /evalue="2.6e-76"
     Typing          612..1120
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.51"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 ggttcatgga tacatgaacc gcctgatgtg gaacgctttt tatctatctc atgcgctgcc
       61 aaagatagat tcattcttta aaagtcaggt tacattatcc cttaaatggt catacaccat
      121 acgcaaccgc gattttcatc gcaattgcat gaattaaaag ataaaaactg aagtccccgt
      181 ttatttatgt aatacgcaac gtattacaac cacctgtaat tacatgaaaa taaagaaata
      241 agatgtaata ccggcgtatt accacgtacg cgggtaagcg aaaaagctat aacgcttccc
      301 ggccgacagg cggcccaaaa tccaccgggg catgcaggtt ctcttcagta acctgcgcca
      361 ttaaatcaaa aaggtcatat tcgccgggaa gctgcgtatc agtcgaatcc agttgttcat
      421 catgttcaga tggcatgggg tgatatccag tagctgagag gttgttagag ggtttcgggg
      481 cgcagccctg aaccagtcac gtagcgctag cggagtgtat actggcttac tatgttggca
      541 ctgatgaggg agtcagtgaa gtgcttcatg tagcaggaca aaaaaggcgg caccggtgcg
      601 tcagcagaat atgtgataca ggatatattc cgcttcctcg ctcactgact cgctacgctc
      661 ggtcgttcga ctgcggcgag cggaaatggc ttacgaacgg ggcggagatg tcctggaaga
      721 tgccagggaa atacttaaca gggaagcgat agggccgcgg caaagccgtt tttccatagg
      781 ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg
      841 acaggactat aaagatacca ggcgtttccc cctggcggct ccctcgtgcg ctctcctgtt
      901 cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc tcattccacg
      961 cctgacactc agttccggga aggcagttcg ctccaagctg gactgtatgc acgaaccccc
     1021 cgttcagtcc gactgctgcg ccttatccgg taactatcgt cttgagtcca acccggaaag
     1081 acacgacaaa gcgccactgg cagcagccac tggtaacagg attagcagag cgagttaatg
     1141 taggtggtgc tatagagttc ttgaagtggt ggcctaacta cggctacact ggaaggacag
     1201 tatttggtat ctgcgctctg ctgaagccag ttaccaggtt aagcagttcc ccaactgact
     1261 taaccttcga aaaaccacct ccccaggtgg ttttttcgtt ttcagagcaa gagattacgc
     1321 gtagaaaaaa aggaactcaa gaagatcctt tctatgctca accaccgcat ggcccatagc
     1381 cgcgccggcc gttgccggtc agctccagaa tcctcgacca atatggaacc ttgcccctcc
     1441 taaaattcaa ctaaaaaacc ctcatttcct gccgataaaa tgggtatctc ttaactatgc
     1501 ctaaataaat aaggataatt ttgaaaaaaa tattcatcct atcggcagcg ctgatcatgg
     1561 caggatgcgc aacaaagcaa tatccacagg ccgtgaacgt caccggtcca gaatcttccg
     1621 caatggattg caaagcggta gctctggaaa tcgccaaaac gcgcagtgta caacaagaaa
     1681 ttgaggaaac cggcgaattt gacgggcgca ccgtattagg tttcctgggt gattttggga
     1741 taggaaatgg catggcaaaa ggcgaagccc gcaaaaaagc agctaaccgg ctaaatcagc
     1801 tggaagccct gaaggctgca aagtgtgcag atcaataagg tacaaaaaag ccctgcgggg
     1861 ctttttttgt acctgccaat cagctgaatt taccataaca tccgttggcc gcaccgcctc
     1921 agaggccctc agcgcgtttc tgacggaaaa ccggtaaaac aggcaacaaa ccacccgaaa
     1981 acccgccaga aacgcgcagg ctctgttttt acggcatgca tgagtatgcg cgcaagtgca
     2041 tagcgcggaa attcacgcca ttcagccggg gtaagtcctg tttccgatgt tcttcttatc
     2101 cgaaaacggt gaacggggac gcaatttatt gcgtggccgt gaggcattga ccaagccgta
     2161 aggcacggta ggtaattgca ggtatccgac aatttcgagt agagcgggtc cgcttggaaa
     2221 aaggaaatta tcctacgtta agcgttttaa gctaacgggt gatttcgttc cattgagcgc
     2281 gaacccctga ccgtaaggga tgtacgaaat gatccggctg tccgttaatg gttttgctta
     2341 ctttcggaca tgttgtctac atgctgtacg taccctaaaa gcaccaaaga cagcatcaca
     2401 gacgcagaga aagcagaact gaagaagctg acccaacgac tgaaagacga ggtgtaagat
     2461 gagttttttt gacgagctga aagcctctct ggaagaggcc gttgaaatca aaaacggaac
     2521 caaagccccc gcccgtgtga cccgctacga gctggcagac gtcagggcta tcagagaaaa
     2581 cctgaatatt actcagagcg aaatggcgaa agtgctggga accagcctcg ataccatcaa
     2641 aagctgggaa accggacgcc gtaacccaac cggactggca gcaaaagtgc tggccaccat
     2701 tcaggctaac ccaaagtttt ttgatgaact ggcggcgcat taagctgatg tgacagaaga
     2761 aattcgacag gcgaggccgt tcgcgtgtaa ctttaacggc aacagaaaaa acaaagcccc
     2821 gataatcatg atttcaactt ggcggaagag acatgataaa tcagggctag gtataaaacc
     2881 taagaaggat attagcacat gaaccgtgcc agacaacagc ccgccataag ttcagggcac
     2941 attgcgtaac gcagcactcg aacttttcaa cgaccgcttg ccgcataaac cgtacttctc
     3001 ggatgattta cactttggtg tccgcatcgc agggaaagag cgcgctattc tggctaaata
     3061 catccagttt aatcagcctc acgccatgtt ctggctggga tttgacgttg accgcatcgg
     3121 cgcagccatt gactggagcg accgcaacgc ccccgcgccg acactgacca tcaccaaccc
     3181 cgaaaacgga cacgcgcacc tgctgtacgc actgaaaacc tcgatacgca ccgcgccaga
     3241 cggaaaaatg aaaccgttac gttacgccgc tgctgtagag agcgcgctac gtaaaaaatt
     3301 gggcgctgac acggggtatt cggggctaat ttgcaagaat ccgaaccaca gtcactggaa
     3361 aattgcccgt atggcagcct gaactctaca cgctggactg gttatccctc ctgttcagct
     3421 actgaaggcg tggtgcgtaa cggtaaaagc actaccggac atcaacgcta tctctgctct
     3481 cactgtcgta aaacatagca agcaactaca gttcacttac accgcctctg agcccggtac
     3541 acaccagaaa atcattgata tggccatgaa tggcgttgga tgccgggcca catcccgcat
     3601 tatgggcact gtcctcaaca cgattttacg acacttaaaa aaattaggcc gcagtcggta
     3661 tcgggcatta tctgaacata aaacacaagc agtaagttgg agtcattacc aattcattta
     3721 atgaatttaa taaatgaatc actttaactt ggtcatgcat cttaacttat gaattactcc
     3781 tgtcctaaca acctcattga aattaaacaa ggtataaaaa acaatataac taaaaatacc
     3841 caatgcgaca ttaaaagaaa aatcaatgct ggcatttttg aaaaaggaaa aatgctgcca
     3901 tactttaata agtaagaaca tcaaaaaagc tggggagata gttctaaaaa acacgtttac
     3961 caattcaatg taactaaact tcaagatatt cttaagtaaa ataaatgccg ggagtgaatt
     4021 aattaagttt gcaaaaaaat aaaggtagac caatgttttt atatcgaaat gaacacctat
     4081 aatgaaagac aaccccatca aaaaagcgcc aatgcaacct aagaaaaaca accaccttgt
     4141 ttttccgtag gccataaaaa cagaacctgt tgtactcaag gctgattgga taatcgctgt
     4201 aggtgccaac caatgcaata agtctccaag taggtgccac ttaggatcaa agaaaatcct
     4261 cgtaaaagaa aaagatgtag cagataaacc tagcatcagt ggcgcgctta atgatagtat
     4321 aagttttaat gcatttaagt aatcttgttt attagtgtta ttagtgttat ttatgttttt
     4381 gctaaggtgt ggtaagaaaa ctctagaaaa aacaagcgtc aggctctgca acggaaataa
     4441 cataatgcgg taggcaatcg aatagacccc aagtattgcg gatgagaaaa aacggcctat
     4501 taaaatagaa tcaagattcc tggaaaaata atttattaaa ttaaaagata ataaataacc
     4561 actaaaccct ataactcctg acttgtcact atggctgata tttttaattc tcaatgataa
     4621 actcaacccg gaaaccttca aaaaacatat tgtggatatt gatattgttg tcagtgtttg
     4681 aaaaatcaaa ctataaacac caaagcctaa attagctaaa actattgcca gcaccattcc
     4741 tgtaaggcta gagaaaattt caattgcaat gatgacttta aagttcattt ctttttctaa
     4801 ttttgctttg tgagttattg ttaagctgga aattggaaag ctaaaagcca atagaaatag
     4861 aaccgggtat agctcgggtg tgttaaagta cacgacaatt aatggggatg ttattattac
     4921 aagcagcata acaaacaacc cagtaaaaat gtttatcctg tatatgtaat tataaaaacc
     4981 gatcgacgcc tcttgtcgct gaattactgc cgccccagtt cccatgtcat tgaatatgct
     5041 tgctaaattc ataaccacag atgacattgc taatagtcca tagctcgatg gggggattaa
     5101 gtgcgtcaag tagaataacg aaaccaattg tgttgctatt tttacaagtt gtccaatgcc
     5161 aacccattta aaactactga ctagtgcagt catattgttt ttaatatcct tttgaatttt
     5221 caataattta taatccgtga tggttttaaa gataccacca taaaagacat cataattctt
     5281 caagctatca tctacgttgt agattccgat ttagctaaac tttaggactt tataagttcg
     5341 tatcccgtat gattataagg cattatgatc atctccacca atagtgggta atgctgccaa
     5401 cttactgatt tagtgtatga tggtaatttt aaggtgcttg cgtggcttcc atttccatca
     5461 gatgtccttc ctgctccgct actgaaggcg tggtgcgtaa cggcaaaagc actgccggac
     5521 atcagcgcta tctctgctct cattgccgta aaacatggca actacagttc acttacaccg
     5581 cctctcagcc cggtacgcac cagaaaatca ttgatatggc catgaatggc gtcggatgtc
     5641 gcgccagtgc acgcattatg ggcgttggcc tcaacacggt tttacgtcac ttaaaaaact
     5701 caggccgcag tcggtaacct cgcgcataca accgggcagt gatgtgattg tctgcgctga
     5761 aatggacgaa cagtggggct acgtcggtgc taaatcacgt cagcgctggc tgttttacgc
     5821 gtatgacagg atacgaagga cggttgtggc gcacgtcttc ggtgaacgca ctctggccac
     5881 actggagcgt cttctgagcc tgctgtcggc ctttgaggtc gtggtatgga tgacggatgg
     5941 ctggccgctg tatgaatcac gcctgaaggg aaagctgcac gttatcagca agcgttacac
     6001 tcagcgcatt gagcgacata atctgaatct gaatctgaga caacatctgg caaggctggg
     6061 acggaagtca ctgtcgttct caaaatcggt ggagctgcat gacaaggtca tcgggcatta
     6121 tctgaacata aaacactatc agtaagttgg agtcattacc cggtctgcgt tgtcgggaag
     6181 atgcgtgatc tggggtcagt ttggatatag agaattattg tacggtaagc ctgttttttg
     6241 aacagactta cctatagcaa tagggtgtac tcccgcgcta tacgacattt tcagaggttt
     6301 gaccttaatc ggataaccta aatgcgatac tgcaagagcg acaacttaaa agcgatagcc
     6361 actgacgcaa aatcaacccc ttacgaacca agattggtta aataatgcgc ttaacgtaca
     6421 aaaatttccg atctccaaac tgaccccgat ctgatccttc aactcagcaa aagttcgatt
     6481 tattcaacaa agccccggaa agcgtgcgta tcgcctgaaa acccaacccg tcacggggat
     6541 acctatccga aatcttattt attcaacaaa gaaaaacctt tattaattat cgctttgctt
     6601 ttattaaggg ttgctttttt tattaataat gccattcctt aggtaattaa acaccttccc
     6661 gttttttagt tttaaataag tctttttaat taatgggtaa ttaattgata cgctatcttt
     6721 aaatgatagg ccggggtgtt ttttatatct cgccacatag ttcattgctt cctttgtttt
     6781 tattgtttcc gatatagaga ataacgacaa aaccattcca ttcacaaatc tatcaaagct
     6841 cttgttttgt atgcctttta tttttttata cttcgtaagc tcatcgatgc atgttgaata
     6901 tccaatgatg tgcctcattg attttttaga cgtcattacg gaatcatttc ttaccctcct
     6961 aagaaataac tcctcatcca acgagaggca tatagttgaa taattgtcaa aaatcagggt
     7021 tgctataaaa tgattatcct catgatatat atgcggaatg aatgcattgc catttaataa
     7081 ctccctttta aaaacatagc aacatggtga cactattagt tttttgttcg ccatttcttt
     7141 ttcaaagaaa gttaatgcat ctatttcgcc aaatgttgag cgcttataat catgccagcc
     7201 tttttttttc agatctggag tgtcaaaaaa aacatgtgca ttaaagaaaa taatcccata
     7261 atttttttca ttggttatct tgtctattaa ctttagactt ccatgtgata ttttatcatc
     7321 accatctaaa aaccatatat attcaccagt tgatgattca atggcgacat ttcttgccac
     7381 cgattggcct tgattttcct gatttattac tttgattctt tggtcatcaa atgacagtat
     7441 gatatctaaa ctgccatctg ttgaaccatc atttacacag attatctcat agtcgttaaa
     7501 atcctgatct aatactgatt gaatacattc ccttaagtat ttttcaacgt tatatacagg
     7561 tactattatc gaaaattttt ttttcacagt aatctccaaa taatatttag ttatactaac
     7621 atcaagttgt tatttatgga atgcgattta tcaacggctt tgttgaataa atcagaatcc
     7681 tttcttcctg ctaaggctat ctctgaatga ctcaaccact ctttgagatt cttcttctgc
     7741 tcgcatgaag tcattcccgg acagaaaaaa accattgtca ggcccactgg cttcagagaa
     7801 attcgtttct gcagcagaca cagaatccac atcagcaaga gacggttcat ctgtactttt
     7861 tctcaatctg taaagagcct tatcaaacgc aaccagtgag atattcaggc cgtaacgttc
     7921 attcagcaca gtcacaatct cttccctacg aataccattt ttcttccagg tttctatatc
     7981 gttcttggaa tccctgacag ctgcaatggc tgttcttgga ccagggttaa aattcgccat
     8041 ataccgccca taatgtctta catttgtctc ataatagtcc aaaataagag taaaacgcaa
     8101 aaaatgtctt acaattgtct tatttttgtc ctttcc
//
