LOCUS       CP058038                8136 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB03-C08 plasmid pRHB03-C08_3, complete
            sequence.
ACCESSION   CP058038
VERSION     CP058038.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15147977
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8136)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 8136)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH01 T1-C08
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 102.885002925307x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 13:14:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,392
            CDSs (total)                      :: 4,274
            Genes (coding)                    :: 4,026
            CDSs (with protein)               :: 4,026
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 248
            CDSs (without protein)            :: 248
            Pseudo Genes (ambiguous residues) :: 0 of 248
            Pseudo Genes (frameshifted)       :: 115 of 248
            Pseudo Genes (incomplete)         :: 111 of 248
            Pseudo Genes (internal stop)      :: 45 of 248
            Pseudo Genes (multiple problems)  :: 21 of 248
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB03-C08"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB03-C08_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(8099..8136,1..94))
                     /locus_tag="HVV60_21900"
     CDS             complement(join(8099..8136,1..94))
                     /locus_tag="HVV60_21900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QMQ56535.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHDKAPLAAATGNRISRAS"
                     /name="replication initiation protein"
     gene            665..814
                     /locus_tag="HVV60_21905"
     CDS             665..814
                     /locus_tag="HVV60_21905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMQ56529.1"
                     /translation="MPSEHDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            1197..1565
                     /locus_tag="HVV60_21910"
     CDS             1197..1565
                     /locus_tag="HVV60_21910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMQ56530.1"
                     /translation="MANFNPGPRTAIAAVRDSKNDIETWKKNGIRREEIVTVLNERYGL
                     NISLVAFDKALYRLRKSTDEPSLADVDSVSAAETNFSEASGPDNGFFLSGNDFMRAEEE
                     SQRVVESFRDSLSRKKGF"
                     /name="hypothetical protein"
     gene            1650..2633
                     /locus_tag="HVV60_21915"
     CDS             1650..2633
                     /locus_tag="HVV60_21915"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="QMQ56531.1"
                     /translation="MKKKFSIIVPVYNVEKYLRECIQSVLDQDFNDYEIICVNDGSTDG
                     SLDIILSFDDQRIKVINQENQGQSVARNVAIESSTGEYIWFLDGDDKISHGSLKLIDKI
                     TNEKNYGIIFFNAHVFFDTPDLKKKGWHDYKRSTFGEIDALTFFEKEMANKKLIVSPCC
                     YVFKRELLNGNAFIPHIYHEDNHFIATLIFDNYSTICLSLDEELFLRRVRNDSVMTSKK
                     SMRHIIGYSTCIDELTKYKKIKGIQNKSFDRFVNGMVLSLFSISETIKTKEAMNYVARY
                     KKHPGLSFKDSVSINYPLIKKTYLKLKNGKVFNYLRNGIINKKSNP"
                     /name="glycosyltransferase family 2 protein"
     gene            complement(3092..3795)
                     /locus_tag="HVV60_21920"
                     /pseudo=""
     CDS             complement(3092..3795)
                     /locus_tag="HVV60_21920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            4044..5498
                     /locus_tag="HVV60_21925"
     CDS             4044..5498
                     /locus_tag="HVV60_21925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="QMQ56532.1"
                     /translation="MTALVSSFKWVGIGQLVKIATQLVSLFYLTHLIPPSSYGLLAMSS
                     VVMNLASIFNDMGTGAAVIQRQEASIGFYNYIYRINIFTGLFVMLLVIITSPLIVVYFN
                     TPELYPVLFLLAFSFPISSLTITHKAKLEKEMNFKVIIAIEIFSSLTGMVLAIVLANLG
                     FGVYSLIFQTLTTISISTICFLKVSGLSLSLRIKNISHSDKSGVIGFSGYLLSFNLINY
                     FSRNLDSILIGRFFSSAILGVYSIAYRIMLFPLQSLTLVFSRVFLPHLSKNINNTNNTN
                     KQDYLNALKLILSLSAPLMLGLSATSFSFTRIFFDPKWHLLGDLLHWLAPTAIIQSALS
                     TTGSVFMAYGKTRWLFFLGCIGAFLMGLSFIIGVHFDIKTLVYLYFFANLINSLPAFIL
                     LKNILKFSYIELVNVFFRTISPAFLMFLLIKVWQHFSFFKNASIDFSFNVALGIFSYIV
                     FYTLFNFNEVVRTGVIHKLRCMTKLK"
                     /name="lipopolysaccharide biosynthesis protein"
     gene            complement(5591..5858)
                     /locus_tag="HVV60_21930"
                     /pseudo=""
     CDS             complement(5591..5858)
                     /locus_tag="HVV60_21930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558268.1"
                     /note="frameshifted; internal stop; incomplete; partial on
                     complete genome; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(5830..6295)
                     /locus_tag="HVV60_21935"
                     /pseudo=""
     CDS             complement(5830..6295)
                     /locus_tag="HVV60_21935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517860.1"
                     /note="frameshifted; incomplete; partial on complete
                     genome; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(6494..6778)
                     /locus_tag="HVV60_21940"
     CDS             complement(6494..6778)
                     /locus_tag="HVV60_21940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QMQ56533.1"
                     /translation="MSFFDELKASLEEAVEIKNGTKAPARVTRYELADVRAIRENLNIT
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFDELAAH"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(7399..7716)
                     /locus_tag="HVV60_21945"
     CDS             complement(7399..7716)
                     /locus_tag="HVV60_21945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMQ56534.1"
                     /translation="MKKIFILSAALIMAGCATKQYPQAVNVTGPESSAMDCKAVALEIA
                     KTRSVQQEIEETGEFDGRTVLGFLGDFGIGNGMAKGEARKKAANRLNQLEALKAAKCAD
                     Q"
                     /name="hypothetical protein"
     Typing          457..630
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.571"
                     /coverage="100.0"
                     /evalue="2.6e-76"
     Typing          complement(2..490)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.694"
                     /coverage="93.0"
                     /evalue="0.0"
ORIGIN
        1 ccagtggcgc tttgtcgtgt ctttccgggt tggactcaag acgatagtta ccggataagg
       61 cgcagcagtc ggactgaacg gggggttcgt gcatacagtc cagcttggag cgaactgcct
      121 tcccggaact gagtgtcagg cgtggaatga gacaaacgcg gccataacag cggaatgaca
      181 ccggtaaacc gaaaggcagg aacaggagag cgcacgaggg agccgccagg gggaaacgcc
      241 tggtatcttt atagtcctgt cgggtttcgc caccactgat ttgagcgtca gatttcgtga
      301 tgcttgtcag gggggcggag cctatggaaa aacggctttg ccgcggccct atcgcttccc
      361 tgttaagtat ttccctggca tcttccagga catctccgcc ccgttcgtaa gccatttccg
      421 ctcgccgcag tcgaacgacc gagcgtagcg agtcagtgag cgaggaagcg gaatatatcc
      481 tgtatcacat attctgctga cgcaccggtg ccgccttttt tgtcctgcta catgaagcac
      541 ttcactgact ccctcatcag tgccaacata gtaagccagt atacactccg ctagcgctac
      601 gtgactggtt cagggctgcg ccccgaaacc ctctaacaac ctctcagcta ctggatatca
      661 ccccatgcca tctgaacatg atgaacaact ggattcgact gatacgcagc ttcccggcga
      721 atatgacctt tttgatttaa tggcgcaggt tactgaagag aacctgcatg ccccggtgga
      781 ttttgggccg cctgtcggcc gggaagcgtt atagcttttt cgcttacccg cgtacgtggt
      841 aatacgccgg tattacatct tatttcttta ttttcatgta attacaggtg gttgtaatac
      901 gttgcgtatt acataaataa acggggactt cagtttttat cttttaattc atgcaattgc
      961 gatgaaaatc gcggttgcgt atggtgtatg accatttaag ggataatgta acctgacttt
     1021 taaagaatga atctatcttt ggcagcgcat gagatagata aaaagcgttc cacatcaggc
     1081 ggttcatgta tccatgaacc ggaaaggaca aaaataagac aattgtaaga cattttttgc
     1141 gttttactct tattttggac tattatgaga caaatgtaag acattatggg cggtatatgg
     1201 cgaattttaa ccctggtcca agaacagcca ttgcagctgt cagggattcc aagaacgata
     1261 tagaaacctg gaagaaaaat ggtattcgta gggaagagat tgtgactgtg ctgaatgaac
     1321 gttacggcct gaatatctca ctggttgcgt ttgataaggc tctttacaga ttgagaaaaa
     1381 gtacagatga accgtctctt gctgatgtgg attctgtgtc tgctgcagaa acgaatttct
     1441 ctgaagccag tgggcctgac aatggttttt ttctgtccgg gaatgacttc atgcgagcag
     1501 aagaagaatc tcaaagagtg gttgagtcat tcagagatag ccttagcagg aagaaaggat
     1561 tctgatttat tcaacaaagc cgttgataaa tcgcattcca taaataacaa cttgatgtta
     1621 gtataactaa atattatttg gagattactg tgaaaaaaaa attttcgata atagtacctg
     1681 tatataacgt tgaaaaatac ttaagggaat gtattcaatc agtattagat caggatttta
     1741 acgactatga gataatctgt gtaaatgatg gttcaacaga tggcagttta gatatcatac
     1801 tgtcatttga tgaccaaaga atcaaagtaa taaatcagga aaatcaaggc caatcggtgg
     1861 caagaaatgt cgccattgaa tcatcaactg gtgaatatat atggttttta gatggtgatg
     1921 ataaaatatc acatggaagt ctaaagttaa tagacaagat aaccaatgaa aaaaattatg
     1981 ggattatttt ctttaatgca catgtttttt ttgacactcc agatctgaaa aaaaaaggct
     2041 ggcatgatta taagcgctca acatttggcg aaatagatgc attaactttc tttgaaaaag
     2101 aaatggcgaa caaaaaacta atagtgtcac catgttgcta tgtttttaaa agggagttat
     2161 taaatggcaa tgcattcatt ccgcatatat atcatgagga taatcatttt atagcaaccc
     2221 tgatttttga caattattca actatatgcc tctcgttgga tgaggagtta tttcttagga
     2281 gggtaagaaa tgattccgta atgacgtcta aaaaatcaat gaggcacatc attggatatt
     2341 caacatgcat cgatgagctt acgaagtata aaaaaataaa aggcatacaa aacaagagct
     2401 ttgatagatt tgtgaatgga atggttttgt cgttattctc tatatcggaa acaataaaaa
     2461 caaaggaagc aatgaactat gtggcgagat ataaaaaaca ccccggccta tcatttaaag
     2521 atagcgtatc aattaattac ccattaatta aaaagactta tttaaaacta aaaaacggga
     2581 aggtgtttaa ttacctaagg aatggcatta ttaataaaaa aagcaaccct taataaaagc
     2641 aaagcgataa ttaataaagg tttttctttg ttgaataaat aagatttcgg ataggtatcc
     2701 ccgtgacggg ttgggttttc aggcgatacg cacgctttcc ggggctttgt tgaataaatc
     2761 gaacttttgc tgagttgaag gatcagatcg gggtcagttt ggagatcgga aatttttgta
     2821 cgttaagcgc attatttaac caatcttggt tcgtaagggg ttgattttgc gtcagtggct
     2881 atcgctttta agttgtcgct cttgcagtat cgcatttagg ttatccgatt aaggtcaaac
     2941 ctctgaaaat gtcgtatagc gcgggagtac accctattgc tataggtaag tctgttcaaa
     3001 aaacaggctt accgtacaat aattctctat atccaaactg accccagatc acgcatcttc
     3061 ccgacaacgc agaccgggta atgactccaa cttactgata gtgttttatg ttcagataat
     3121 gcccgatgac cttgtcatgc agctccaccg attttgagaa cgacagtgac ttccgtccca
     3181 gccttgccag atgttgtctc agattcagat tcagattatg tcgctcaatg cgctgagtgt
     3241 aacgcttgct gataacgtgc agctttccct tcaggcgtga ttcatacagc ggccagccat
     3301 ccgtcatcca taccacgacc tcaaaggccg acagcaggct cagaagacgc tccagtgtgg
     3361 ccagagtgcg ttcaccgaag acgtgcgcca caaccgtcct tcgtatcctg tcatacgcgt
     3421 aaaacagcca gcgctgacgt gatttagcac cgacgtagcc ccactgttcg tccatttcag
     3481 cgcagacaat cacatcactg cccggttgta tgcgcgaggt taccgactgc ggcctgagtt
     3541 ttttaagtga cgtaaaaccg tgttgaggcc aacgcccata atgcgtgcac tggcgcgaca
     3601 tccgacgcca ttcatggcca tatcaatgat tttctggtgc gtaccgggct gagaggcggt
     3661 gtaagtgaac tgtagttgcc atgttttacg gcaatgagag cagagatagc gctgatgtcc
     3721 ggcagtgctt ttgccgttac gcaccacgcc ttcagtagcg gagcaggaag gacatctgat
     3781 ggaaatggaa gccacgcaag caccttaaaa ttaccatcat acactaaatc agtaagttgg
     3841 cagcattacc cactattggt ggagatgatc ataatgcctt ataatcatac gggatacgaa
     3901 cttataaagt cctaaagttt agctaaatcg gaatctacaa cgtagatgat agcttgaaga
     3961 attatgatgt cttttatggt ggtatcttta aaaccatcac ggattataaa ttattgaaaa
     4021 ttcaaaagga tattaaaaac aatatgactg cactagtcag tagttttaaa tgggttggca
     4081 ttggacaact tgtaaaaata gcaacacaat tggtttcgtt attctacttg acgcacttaa
     4141 tccccccatc gagctatgga ctattagcaa tgtcatctgt ggttatgaat ttagcaagca
     4201 tattcaatga catgggaact ggggcggcag taattcagcg acaagaggcg tcgatcggtt
     4261 tttataatta catatacagg ataaacattt ttactgggtt gtttgttatg ctgcttgtaa
     4321 taataacatc cccattaatt gtcgtgtact ttaacacacc cgagctatac ccggttctat
     4381 ttctattggc ttttagcttt ccaatttcca gcttaacaat aactcacaaa gcaaaattag
     4441 aaaaagaaat gaactttaaa gtcatcattg caattgaaat tttctctagc cttacaggaa
     4501 tggtgctggc aatagtttta gctaatttag gctttggtgt ttatagtttg atttttcaaa
     4561 cactgacaac aatatcaata tccacaatat gttttttgaa ggtttccggg ttgagtttat
     4621 cattgagaat taaaaatatc agccatagtg acaagtcagg agttataggg tttagtggtt
     4681 atttattatc ttttaattta ataaattatt tttccaggaa tcttgattct attttaatag
     4741 gccgtttttt ctcatccgca atacttgggg tctattcgat tgcctaccgc attatgttat
     4801 ttccgttgca gagcctgacg cttgtttttt ctagagtttt cttaccacac cttagcaaaa
     4861 acataaataa cactaataac actaataaac aagattactt aaatgcatta aaacttatac
     4921 tatcattaag cgcgccactg atgctaggtt tatctgctac atctttttct tttacgagga
     4981 ttttctttga tcctaagtgg cacctacttg gagacttatt gcattggttg gcacctacag
     5041 cgattatcca atcagccttg agtacaacag gttctgtttt tatggcctac ggaaaaacaa
     5101 ggtggttgtt tttcttaggt tgcattggcg cttttttgat ggggttgtct ttcattatag
     5161 gtgttcattt cgatataaaa acattggtct acctttattt ttttgcaaac ttaattaatt
     5221 cactcccggc atttatttta cttaagaata tcttgaagtt tagttacatt gaattggtaa
     5281 acgtgttttt tagaactatc tccccagctt ttttgatgtt cttacttatt aaagtatggc
     5341 agcatttttc ctttttcaaa aatgccagca ttgatttttc ttttaatgtc gcattgggta
     5401 tttttagtta tattgttttt tataccttgt ttaatttcaa tgaggttgtt aggacaggag
     5461 taattcataa gttaagatgc atgaccaagt taaagtgatt catttattaa attcattaaa
     5521 tgaattggta atgactccaa cttactgctt gtgttttatg ttcagataat gcccgatacc
     5581 gactgcggcc taattttttt aagtgtcgta aaatcgtgtt gaggacagtg cccataatgc
     5641 gggatgtggc ccggcatcca acgccattca tggccatatc aatgattttc tggtgtgtac
     5701 cgggctcaga ggcggtgtaa gtgaactgta gttgcttgct atgttttacg acagtgagag
     5761 cagagatagc gttgatgtcc ggtagtgctt ttaccgttac gcaccacgcc ttcagtagct
     5821 gaacaggagg gataaccagt ccagcgtgta gagttcaggc tgccatacgg gcaattttcc
     5881 agtgactgtg gttcggattc ttgcaaatta gccccgaata ccccgtgtca gcgcccaatt
     5941 ttttacgtag cgcgctctct acagcagcgg cgtaacgtaa cggtttcatt tttccgtctg
     6001 gcgcggtgcg tatcgaggtt ttcagtgcgt acagcaggtg cgcgtgtccg ttttcggggt
     6061 tggtgatggt cagtgtcggc gcgggggcgt tgcggtcgct ccagtcaatg gctgcgccga
     6121 tgcggtcaac gtcaaatccc agccagaaca tggcgtgagg ctgattaaac tggatgtatt
     6181 tagccagaat agcgcgctct ttccctgcga tgcggacacc aaagtgtaaa tcatccgaga
     6241 agtacggttt atgcggcaag cggtcgttga aaagttcgag tgctgcgtta cgcaatgtgc
     6301 cctgaactta tggcgggctg ttgtctggca cggttcatgt gctaatatcc ttcttaggtt
     6361 ttatacctag ccctgattta tcatgtctct tccgccaagt tgaaatcatg attatcgggg
     6421 ctttgttttt tctgttgccg ttaaagttac acgcgaacgg cctcgcctgt cgaatttctt
     6481 ctgtcacatc agcttaatgc gccgccagtt catcaaaaaa ctttgggtta gcctgaatgg
     6541 tggccagcac ttttgctgcc agtccggttg ggttacggcg tccggtttcc cagcttttga
     6601 tggtatcgag gctggttccc agcactttcg ccatttcgct ctgagtaata ttcaggtttt
     6661 ctctgatagc cctgacgtct gccagctcgt agcgggtcac acgggcgggg gctttggttc
     6721 cgtttttgat ttcaacggcc tcttccagag aggctttcag ctcgtcaaaa aaactcatct
     6781 tacacctcgt ctttcagtcg ttgggtcagc ttcttcagtt ctgctttctc tgcgtctgtg
     6841 atgctgtctt tggtgctttt agggtacgta cagcatgtag acaacatgtc cgaaagtaag
     6901 caaaaccatt aacggacagc cggatcattt cgtacatccc ttacggtcag gggttcgcgc
     6961 tcaatggaac gaaatcaccc gttagcttaa aacgcttaac gtaggataat ttcctttttc
     7021 caagcggacc cgctctactc gaaattgtcg gatacctgca attacctacc gtgccttacg
     7081 gcttggtcaa tgcctcacgg ccacgcaata aattgcgtcc ccgttcaccg ttttcggata
     7141 agaagaacat cggaaacagg acttaccccg gctgaatggc gtgaatttcc gcgctatgca
     7201 cttgcgcgca tactcatgca tgccgtaaaa acagagcctg cgcgtttctg gcgggttttc
     7261 gggtggtttg ttgcctgttt taccggtttt ccgtcagaaa cgcgctgagg gcctctgagg
     7321 cggtgcggcc aacggatgtt atggtaaatt cagctgattg gcaggtacaa aaaaagcccc
     7381 gcagggcttt tttgtacctt attgatctgc acactttgca gccttcaggg cttccagctg
     7441 atttagccgg ttagctgctt ttttgcgggc ttcgcctttt gccatgccat ttcctatccc
     7501 aaaatcaccc aggaaaccta atacggtgcg cccgtcaaat tcgccggttt cctcaatttc
     7561 ttgttgtaca ctgcgcgttt tggcgatttc cagagctacc gctttgcaat ccattgcgga
     7621 agattctgga ccggtgacgt tcacggcctg tggatattgc tttgttgcgc atcctgccat
     7681 gatcagcgct gccgatagga tgaatatttt tttcaaaatt atccttattt atttaggcat
     7741 agttaagaga tacccatttt atcggcagga aatgagggtt ttttagttga attttaggag
     7801 gggcaaggtt ccatattggt cgaggattct ggagctgacc ggcaacggcc ggcgcggcta
     7861 tgggccatgc ggtggttgag catagaaagg atcttcttga gttccttttt ttctacgcgt
     7921 aatctcttgc tctgaaaacg aaaaaaccac ctggggaggt ggtttttcga aggttaagtc
     7981 agttggggaa ctgcttaacc tggtaactgg cttcagcaga gcgcagatac caaatactgt
     8041 ccttccagtg tagccgtagt taggccacca cttcaagaac tctatagcac cacctacatt
     8101 aactcgctct gctaatcctg ttaccagtgg ctgctg
//
