LOCUS       CP058033                6768 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB03-C12 plasmid pRHB03-C12_3, complete
            sequence.
ACCESSION   CP058033
VERSION     CP058033.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15147979
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6768)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 6768)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH01 T1-C12
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 105.800720179989x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 13:53:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,561
            CDSs (total)                      :: 4,439
            Genes (coding)                    :: 4,329
            CDSs (with protein)               :: 4,329
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 110
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 110
            Pseudo Genes (frameshifted)       :: 46 of 110
            Pseudo Genes (incomplete)         :: 48 of 110
            Pseudo Genes (internal stop)      :: 31 of 110
            Pseudo Genes (multiple problems)  :: 13 of 110
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB03-C12"
                     /isolation_source="Pooled pig faecal samples collected from
                     floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB03-C12_3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(6494..6768,1..199))
                     /locus_tag="HVV62_22730"
     CDS             complement(join(6494..6768,1..199))
                     /locus_tag="HVV62_22730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="QMQ52501.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVGR
                     KTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEIYR
                     ETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
                     /name="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
     gene            complement(220..747)
                     /locus_tag="HVV62_22735"
                     /pseudo=""
     CDS             complement(220..747)
                     /locus_tag="HVV62_22735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /name="phosphotransferase"
     gene            complement(808..1623)
                     /gene="sul2"
                     /locus_tag="HVV62_22740"
     ARG             complement(812..1623)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            2812..2961
                     /locus_tag="HVV62_22745"
     CDS             2812..2961
                     /locus_tag="HVV62_22745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMQ52503.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            complement(3706..4146)
                     /locus_tag="HVV62_22750"
     CDS             complement(3706..4146)
                     /locus_tag="HVV62_22750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QMQ52504.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="entry exclusion protein 1"
     gene            4364..4753
                     /locus_tag="HVV62_22755"
     CDS             4364..4753
                     /locus_tag="HVV62_22755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMQ52505.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            4797..5201
                     /locus_tag="HVV62_22760"
                     /pseudo=""
     CDS             4797..5201
                     /locus_tag="HVV62_22760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(5308..6144)
                     /locus_tag="HVV62_22765"
     CDS             complement(5308..6144)
                     /locus_tag="HVV62_22765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="QMQ52506.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            complement(6144..6419)
                     /locus_tag="HVV62_22770"
                     /pseudo=""
     CDS             complement(6144..6419)
                     /locus_tag="HVV62_22770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
                     /name="aminoglycoside phosphotransferase APH(3')"
     ARG             complement(5312..6144)
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(6495..6766)
                     /name="dfrA14"
                     /gene="dfrA14"
                     /gene_name="trimethoprim resistant dihydrofolate reductase
                     dfr"
                     /tool="rgi"
                     /drug_class="diaminopyrimidine antibiotic"
                     /identity="100.0"
                     /coverage="57.32"
     ARG             complement(3..208)
                     /name="dfrA14"
                     /gene="dfrA14"
                     /gene_name="trimethoprim resistant dihydrofolate reductase
                     dfr"
                     /tool="rgi"
                     /drug_class="diaminopyrimidine antibiotic"
                     /identity="96.97"
                     /coverage="43.95"
     ARG             complement(6145..6389)
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="30.34"
     ARG             complement(206..747)
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="99.43"
                     /coverage="67.42"
     Typing          2604..2777
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          complement(2075..2680)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 aacctgagcg ggtaacgacc gcgtatttcc tattggggag tgcgcccata gattcaaacg
       61 tcttgcgacc caccagaagc cactgattgt aggtcaatgc tttaaaaagt agctgctccc
      121 ctttcgcgga ccagggtatg tctggaccgc aaccaatcac gccgtttttc gctttcgcag
      181 ctatcaatga tactttcaag gttctcatcc tgggttaacg gtgcgctctt ggtcgagccg
      241 caccggtagc tctcgttcga cacgagccaa aagatcgagc tgcggcgtac tcttgtcctc
      301 gtccggtaag aagtcgggat tgacggcatt gcgggacacc acatcaacgg cgcgtccgaa
      361 cattcgcgac agcctgcgct caaacggaca ttgatcaacc gataggctgt gaacagcgcc
      421 aagttgctgc cccattgacg gccacgcttt gagcaaatcc gctccagaca gatcagccgc
      481 cggtactccc ggaattgccg ttatcaccaa gcatgcaccc tcctgttcct cctgccagtt
      541 gatgacctcg gggcaagcca cacctcgacc tttgagccaa atgaggcggt cacgctctcc
      601 agcgagctca ccgcggcggg aagcaggtgc gattttcgcg aaggcatgcc cgtcaccacg
      661 tcgaaaaaca aaatcaccag attctccgcc tctgacaggc aaccagtcag aatgcgattc
      721 accaaaaaaa atattagttc gattcaatgg aggttccttc agttttctga tgaagcgcga
      781 atatagagaa atatcccgaa tgtgcagtta acgaattctt gcggtttctt tcagcgccgc
      841 caataccgcc agcccgtcgc gcaaggggcg cggctcgtgt gtgcggatga agtcagctcc
      901 acctgcggcg gcggcaagct ctgcagcgag tgtcgcggcc ccgacatccc ccggaccacg
      961 gcctgtgagc gcgcgcagaa aggatttgcg cgaaacagac agaagcaccg gcaaatcgaa
     1021 gcgcagccgc aattcatcga accgcgccag caccgagagc gaggtttcgg gagcagcccc
     1081 cagaaaaaac cccatgccgg gatcaaggac aaggcggttg cgtttgatac cggcacccgt
     1141 cagcgccgcg atgcgcgcgt caaagaacgc cgcaatgtga tccatgatgt cgccagcggg
     1201 tgcctcgcgc cgatctgcct gcccgtcttg caccgaatgc ataacgacga gtttggcaga
     1261 tgatttcgcc aattgcggat agaacgcagc gtctggaaaa ccgcgaatat cattgagata
     1321 ggccacacca cgcgacaagg cataggcttg cgtcgcgggt tgataactgt cgagcgagac
     1381 gggaatgcca tctgccttga gcgcgtccag caccggcgcg atacgcgcga tttctgtgtc
     1441 ggacgaaaca ggcgcggcgt cgggattgct ggatgccgga ccgaggtcga tcacatctgc
     1501 cccctcggcc atcagcttac gcgcctgcgc aatggctgcg tctggcgcca gataccggcc
     1561 tccatcggag aaactgtccg aggttatgtt gacgatgccg aaaatgatga gcgatttatt
     1621 catgggggct tctataataa taataatcga gcatgagtct catacggatg ctcgggtcga
     1681 aagggaatcc ccaggcgagt aacctgtttg cggtgatccg tttcatgatg atgtatctgt
     1741 agcattttgg aaataaatat atcatctctc aataagtaga tatatttatc ttttttgctt
     1801 ctttggtgct gcaagtcacc aatacgaggg aaacctgtta cggagcggtt taaccgtaat
     1861 caacagtgac tggtgtcatg ttcggaagat taattgatag taatctacaa aggatcttct
     1921 tgagatcctt ttcaatgaat cgtaatctct tgctctgtaa acgaaaaaac cgcctgggga
     1981 ggcggtttgt tcgaaggtta agtcagttgg ggaactgctt aacccggtaa cagggttcac
     2041 gagaccgcca ttaccaaaac tgttctttca gtgtagccgc agtcaggcct ctacttcaag
     2101 acctcttaat ctatcttctc gtgcactcct gttactaatg gctgctgcca gtggcgtttt
     2161 gtcgtgtctt tccgggttgg actcaagttg atagttaccg gacaaggcgc agcggtcgga
     2221 ctgaacgggg ggttcgtgca tacagtccag cttggagcga actgccttca cggaactgag
     2281 tgtcaggcgt ggaatgagaa accgcggcca taacagcgga atgacaccgg taaaccgaaa
     2341 ggcaggaaca ggagagcgca cgagggagct accaggggga aacgcctggg atctttatag
     2401 tcctgtcggg tttcgccacc actgatttga gcgtcagatt ttgcgatgct tgtcaggggg
     2461 gcggagccta tggaaaaacg gcttcgccgt ggccttatct cttctttgct aagtgtcttc
     2521 ctggtatctt ccaggaaatt tccgtccccg tccgtaagcc ggtaccgctc gccgcagccg
     2581 aacgaccgag cgtagcgagt cagtgagcga ggaagcggaa tatctcctgt atcgcatatt
     2641 ctgctgacgc gccggtgctg ccttttttct cctgccacat gaagcacttc actgattttc
     2701 gcaatcgtgg caacatagta agccagtata cactccgcta gcgctacatg actggttcag
     2761 ggctgcgccc cgaaacccgc taacaacctc tcagctactg gatatcaccc catgccatct
     2821 gaatatgatg aacaactgga ttcgactgat acgcagcttc ccggcgaata tgaccttttt
     2881 gatttaatgg cgcaggttac tgaagagaac ctgcatgccc ctgtggattt tgggtcgcct
     2941 gtcggccggg aagcgttata gctttttcgc ttgcccgcgt acgtggtaat actccggtat
     3001 taccttttat ttctttattt tcatgtaatt acagtggatt tttaataccc gggtattact
     3061 cgtgaaaacg aaatattcct tttttttctt ttaattcatg cagttgcgat gaaaatcgcg
     3121 gctgcgttag gtgtatgacc attttgaggt tgaaaaataa tcaaccatgg cgggtgattt
     3181 acgcaggtta aggggagatc gtgacaaaaa aagcgttcct cactgtatcg ttcatgcctg
     3241 catgaacggg ttattcttcc tgactacaga attacatcgc tccttcagtt catacatcac
     3301 ttttacatca cttatacatc acttttacat cattccttca gctcatacgt cactttcatc
     3361 ctctgtgcca tttgtacatc acccccttcc tgaacgtgct gacagcgggt tttccggctg
     3421 cgccggcaac gcttcacggc cacgcaataa attgcgtccc cgttcaccgt tttcggatgg
     3481 gaagaacgcc ggaaacagga cttaccccgg ctgaatggtg tgaatttccg cgctatgcac
     3541 ttgtgcgcat actcatgcat gccgtaaaaa cagagcctgc gcgtttctgg cgggttttcg
     3601 ggtggtttgt tgcctgtttt accggtttcc cgtcagaaac gccctgaggg cctctcaggc
     3661 ggtgctgata acagatgtta tggtaaatca gagtcacctg cactgttact ctgaaccgga
     3721 gcgaaacagc cgcgaccaga atccccgctt tttttcctgt gccagcgcgt cagccagacg
     3781 gctaatttcg gcctgtagtt gttccctctc ctcatttcgc aggcggtttt ctgcttcccg
     3841 cgccatcttg tcttcaagca ttagggtaac agtctgctgc agagcgtcca gtttctgctg
     3901 aatgacgtct agctgagcat tttccgcctg gctaacgaca gatttggagt gtccctcctt
     3961 gtgccttttt ggtgtctcag aagtgcgcag ctcgccatag gcccgaatca gttcactggt
     4021 ctcgaactcc cggcgatcat tttttcctgt gcgatagctg acaaggcctt tagccatgtc
     4081 acgatataat tgcgagcgcg actttcctgt taatttcagt gtctcttgaa ctgtgtgcca
     4141 tgccataaaa gtgacagtgt ctcctgtgtg taccgtattg taacagcaga acatattact
     4201 gttttaacac aaaaggtctg aggctgcccg tgaggcgtga aaaatacaca aaaaacctgt
     4261 tgacggtgtg gttttgacgg gcgtagtatt tcgaatactg tatataaaca cattgttata
     4321 aatacagttt ttgctgtttt taaaagtcaa agaggataat tttatgagta cagagttaaa
     4381 tagcggtact gtcggtggtt ttggtcacat gggaccaaat gacccctaca atgatggtca
     4441 tagcggctct ggaagttcgg gaggtagttt taacggtggt ggtaatggtg gccatgataa
     4501 tagtagtggt tcatcttata ctgcatcctt tggtctcatc actggccgac acccgataac
     4561 tgtcccaggt acgaagaata ttaattctgt cgatctttct gctgtcatta ataacattat
     4621 acatcatgaa ggtgcacgca ataacatgta cctagatact gcgaacaatg taagagtggg
     4681 agtgacgggc actggctggc aatgtctagc aacggcaggc atttcggctg agggtaaaag
     4741 aactttccgc taagcgatag actgtatgta aacacagtat tgcaaggacg cggaacatgc
     4801 ctcatgtggc ggccaggacg gccagccggg atcgggatac tggtcgttac cagagccacc
     4861 gacccgagca aacccttctc tatcagatcg ttgacgagta ttacccggca ttcgctgcgc
     4921 ttatggcaga gcagggaaag gaattgccgg gctatgtgca acgggaattt gaagaatttc
     4981 tccaatgcgg gcggctggag catggctttc tacgggttcg ctgcgagtct tgccacgccg
     5041 agcacctggt cgctttcagc tgtaagcgtc gcggtttctg cccgagctgt ggggcgcggc
     5101 ggatggccga aagtgccgcc ttgctggttg atgaagtact gcctgaacaa cccatgcgtc
     5161 agtgggtgtt gagcttcccg tttcagctgc gtttcctgtt tggggtcgtt tgcgggaagg
     5221 ggcggaatcc tacgctaagg ctttggccag cgatattctc cggtgagatt gatgtgttcc
     5281 caggggatag gagaagtcgc ttgatatcta gtatgacgtc tgtcgcacct gcttgatcgc
     5341 ggccgcgata gctagatcgc gttgctcctc ttctccatcc gcgttccaag ctgcggaaag
     5401 gcacccataa gcgtacgcct ggtcgagcag gcgacgcgga tcgacgtcca gcgcacgaga
     5461 gaatgcgtcc gccatctgtg caatgcgtct aggatcgaga caaaggtcgt ctctgtcagc
     5521 cggatcgtag aacatattgg cggcgccaaa gcccacttca ccgaccagac cgacgggatc
     5581 tatcaccagc cagccgcgac tggagaacat gatgttttca tgatgcagat cgccatgtag
     5641 cccacgcagt tccgaggcat tgctcatcat ttgatcggct ataatcgccg cgtggacgta
     5701 gtcagtttga caacctgcgt tttgatcatc gcgcgcccgc tgaaacaaag ctgcaaagcg
     5761 atcccggatc gggagaaggg cagaaggcag gggttcctca gatgcggcat acagcttcgc
     5821 cattagttcc gctgcaattt cggtcgcctg gtagtcgccg tgctcggcaa cgatgtgaga
     5881 gagcattcgc tccccggcat attcgagcaa catcagattg ttctcacgac cgagcaaccg
     5941 gactgctccc ctcccattgc gccataccag atagtcggcc ccgcgcagtt catcagcaat
     6001 gtcttctata ggtttcaatc ccttgacgat tgcaggagtc ccgtctggca atgaaacttt
     6061 ccaaacgagg ctggaaaagg tgtccgcaat gagaacaggt tgcgaaacgt gccaatgagc
     6121 aggaaaaaca ggcggcatga acatcaaccc caagtcagag ggtccaatcg cagatagaag
     6181 gcaaggcgtt cgcggtcggg ggcttcgatc cccaatacat tgaataggac agcgaaggcg
     6241 cgctctgctt catctggcgc tgcccagttc tcttcggcgt tagcaatcat gagtgccaaa
     6301 tcggcatagc gatctgctgt tccgagccgc ccaaggtcga tcagacccgt gcattgaaga
     6361 gttttagggt ccaccatgaa gttcggcatg cagggatcac catggcaaac aaccatatcg
     6421 tcgctatcaa tcgcggaaaa aggcgtagtg cgcgaagcga acgaagcttt ttgccgtcga
     6481 ttgcatagct ttgttaaccc tttttccaaa tttgatagca atagttaatg tttgaagtaa
     6541 agtgttgctc aaaaacaact tcgaaggtat ttggaatact cgggaagaaa acatccccct
     6601 ctggctcgat gtcgatcgtc gataagtgga gcgtagaggc catgggtaat gtttctcggt
     6661 aaatttctcc gccaccagac actataacgt gaccggtgaa ttcagctagc ctgtccatgg
     6721 cctcttcgat tgactgaaat acaactacat tgtcatcatt tgatgtcc
//
