LOCUS       CP058022                8873 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB04-C06 plasmid pRHB04-C06_4, complete
            sequence.
ACCESSION   CP058022
VERSION     CP058022.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15147986
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8873)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 8873)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH13 T1-C06
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 98.3835736129314x
            Sequencing Technology :: Hybrid: Illumina and OXFORD_NANOPORE
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 13:51:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,937
            CDSs (total)                      :: 4,809
            Genes (coding)                    :: 4,709
            CDSs (with protein)               :: 4,709
            Genes (RNA)                       :: 128
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 47 of 100
            Pseudo Genes (incomplete)         :: 42 of 100
            Pseudo Genes (internal stop)      :: 29 of 100
            Pseudo Genes (multiple problems)  :: 17 of 100
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB04-C06"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB04-C06_4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(8393..8873,1..110))
                     /locus_tag="HVV69_24625"
     CDS             complement(join(8393..8873,1..110))
                     /locus_tag="HVV69_24625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMQ39152.1"
                     /translation="MLIRDQDKGKRTRLEDGYRSLENKKFQDYTDEDMRKLGTIDVALE
                     LYTTNYEQGSQMALLEMADKINNHEVAIIDNCLCMRRIAPPLPGMIHNKAVLTDPAFAY
                     MWTGYVKVIAVHSLDYKDTFKATDRARYWRMVRDNEKRLNEDLPVGGYDAVDLQGIALA
                     NIRARPDYSDNPHADCEGMFVPEDYVLQAPEQI"
                     /name="hypothetical protein"
     gene            complement(104..523)
                     /locus_tag="HVV69_24630"
     CDS             complement(104..523)
                     /locus_tag="HVV69_24630"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMQ39153.1"
                     /translation="MTDNLPQTPASRTFQTSKGQNGRPTKYNDRIAKQICLYVAEGMSL
                     NEISKIPGMPCRATLIVWQLEHPELRQTIEGLRFIVATDYACQALECFDDLDPASENIR
                     AELAIAKAKAATLLGMARLQDLRNLPRPIQENEEC"
                     /name="hypothetical protein"
     gene            complement(618..938)
                     /locus_tag="HVV69_24635"
     CDS             complement(618..938)
                     /locus_tag="HVV69_24635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMQ39154.1"
                     /translation="MELKDRIIQTKCESDPVFRERYLRAQVKPLAEVQDLMRRGCSDPL
                     AQEKARQAIIDAVDLLLLHPEAAHHLMAVVRAAHEIAREPVPKASEPLNDSDLDQQIED
                     EF"
                     /name="hypothetical protein"
     gene            complement(953..1228)
                     /locus_tag="HVV69_24640"
     CDS             complement(953..1228)
                     /locus_tag="HVV69_24640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMQ39155.1"
                     /translation="MNQYELTVATQSADVRPEAELNRAERIKAALIAIKAELFNMSEED
                     LLQLQQDVNAAVQKRNQVSEENKVAEAGLKVHQHHQANVPSWNDYF"
                     /name="hypothetical protein"
     gene            complement(1225..1524)
                     /locus_tag="HVV69_24645"
     CDS             complement(1225..1524)
                     /locus_tag="HVV69_24645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMQ39156.1"
                     /translation="MLALKVIHGMSREIAERVSFLRHECPYQCQPAKRVALRCFAELVL
                     GTINRLIAGKLKNESSMHECNQEEREFIRRLKLIRNDIHAQLASVGCDVGGDDK"
                     /name="hypothetical protein"
     gene            complement(1668..2048)
                     /locus_tag="HVV69_24650"
     CDS             complement(1668..2048)
                     /locus_tag="HVV69_24650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMQ39157.1"
                     /translation="MKDEIERLVQQFQILESAAAEKTGAERAALQKRAAEIKMEIIKML
                     PKQGQQSESKVPGLDYFDNKLDPAFGDYFSGDYLTDDMRDYFTVKVPDNMPEARQSTET
                     PKSYEMDADVRKRILNAIGIPC"
                     /name="hypothetical protein"
     gene            complement(2069..2368)
                     /locus_tag="HVV69_24655"
     CDS             complement(2069..2368)
                     /locus_tag="HVV69_24655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMQ39158.1"
                     /translation="MSNTYFDRMPKYQPSQEALDKRAFDEEMNKTAERVIELIPDLLAD
                     IIDGEAARLADLVPEVLRKPDPVTHQRFDEKACRRFLAGKVANKLGRGLNWLNK"
                     /name="hypothetical protein"
     gene            complement(2457..2966)
                     /locus_tag="HVV69_24660"
     CDS             complement(2457..2966)
                     /locus_tag="HVV69_24660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="QMQ39159.1"
                     /translation="MPMPTPLIPPPEYQEEIRKHFTVNAKSQTGVDRDGHELCLKPSEH
                     KYVIRRGRKYKRHNKHRYYAVNIRANGKRKRFYVHNIVIWLTYGFDAIKPGFCVNHRDG
                     NGTNNVLSNLEIVPVAVNSAERRKPHEQPRKAATKHKAHWCLRPVTTAMLWKVNQHWQL
                     PFRCRH"
                     /name="HNH endonuclease"
     gene            complement(2969..3166)
                     /locus_tag="HVV69_24665"
     CDS             complement(2969..3166)
                     /locus_tag="HVV69_24665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QMQ39164.1"
                     /translation="MMNKDNDIIGISEVARMFGVHRQTITKWCDTIPDFPSPFTPQVKG
                     IHQKWYRHEIIEYMNRNKER"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(4240..5856)
                     /locus_tag="HVV69_24670"
     CDS             complement(4240..5856)
                     /locus_tag="HVV69_24670"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMQ39160.1"
                     /translation="MRITTGKEETHMNNMPQNSPVDQGEHEKDSTNVVPIDSAKEHKCP
                     TPEELASVVEIAEQQAREREAERQRAAEAAVKAMEPQRVNRDTMKAKVMALDEMLAKCV
                     WIASGERVAFLDEPWRNFAWSEFRNMMAASKTMIDVGGGGGLKMKAVPTTSLWRESDKR
                     MIVDALTCAPGRDVICEDPNGRKCLNLWKKPVWTPDYETDVSAFYDDCRWLFGDKWVTV
                     VNWIAHMIQRPDVLPHWGILHYTNGIQGVGRTTFCEILKALLGRNCASGVNIHDLLEGQ
                     FNSELAGAVLATCDEIREGDNSQYRTANRLKAMITEPIRHINVKYGAKYDEWNACRWFV
                     CSNHDNPLPLDEHDRRWFAIRHTAGKYADVYGPEAARVMAKRLDDLLANPKAINALGWQ
                     LATRDLSGFDIKQPPVCDEDKLRAINSAKPLERVYLDELIADWPSDIISNRDLAEHILG
                     DVGAAITNKQRRMFEDAGMVPTPVVKLAGKAVRGWIIRNHEQWQNAKPVQISSEMGSAR
                     DRAGVAKLAASSDVMVEVTNK"
                     /name="hypothetical protein"
     gene            complement(5947..6198)
                     /locus_tag="HVV69_24675"
     CDS             complement(5947..6198)
                     /locus_tag="HVV69_24675"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMQ39161.1"
                     /translation="MQGQTVKDFVMAMSDVCELLQRSRTKIYDVMRRYPDFPKPENSGR
                     GKMLIWMKADMLEWYEMHKEELIKDLKRSGRKCVELQK"
                     /name="hypothetical protein"
     gene            complement(6330..6974)
                     /locus_tag="HVV69_24680"
     CDS             complement(6330..6974)
                     /locus_tag="HVV69_24680"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QMQ39162.1"
                     /translation="MNKFYRQLRETAERKYPGKHGGIGYQQIAQELRIGTNEISAIFTQ
                     HQKGPHKRPDIVALAKVIQHLGLSRVALAHIYAEEQVTITNEAMENENSVATDKKQDSN
                     KPEPMERGNPKPKKWHVDLLIQALENIGGVYEGNNAGLQSLITIEGKSIPVAGFYRLLA
                     EARDNALIQYDREMIFRHDKDGNEIREENGKRKGKAGRIRVTLQQKAADGE"
                     /name="hypothetical protein"
     gene            complement(7476..8087)
                     /locus_tag="HVV69_24685"
     CDS             complement(7476..8087)
                     /locus_tag="HVV69_24685"
                     /inference="COORDINATES: protein motif:HMM:NF012464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="QMQ39163.1"
                     /translation="MANFAYIRVSTIQQNTDRQYHAMKDIAIDKWFEEKKSGKNEDREQ
                     LKLMLDYAREGDHVYVASLDRLGRNLIGILKMVEDLRERGVVLHCLKEGYVLGAEKNAL
                     QEAFFQMMGVLAQLERNIMLERQAEAYAAAKAAGKQIGRGKSKRRQESEEFVIEALKNG
                     HQPAKIARAHKISRTSVYRIRDQAIKEGKLPENFRVKAIN"
                     /name="recombinase family protein"
ORIGIN
        1 agtttccgca tgtcttcatc ggtgtagtcc tggaacttct tgttctccag cgagcggtaa
       61 ccgtcttcaa ggcgcgtgcg tttacctttg tcctggtcgc ggattaacat tcttcattct
      121 cctgtattgg tcgcggcaga ttacgcaaat cctgtagtcg tgccatgccg agtagcgttg
      181 ccgctttcgc tttggcaatg gctagttcgg cccggatatt ctcgctggct ggatcgaggt
      241 cgtcgaaaca ttccagcgcc tgacaagcgt aatcggtagc gacaatgaat cttaaacctt
      301 cgatagtctg cctcagttcc gggtgttcaa gctgccagac gattaacgtt gcccggcaag
      361 gcattccggg gattttcgag atttcgttga ggctcattcc ttccgcaaca tataagcaaa
      421 tctgttttgc tatgcggtca ttgtattttg ttgggcgtcc attttgccct ttacttgtct
      481 gaaaagtccg gctggcgggc gtttgcggca gattatcagt cataaatcac ctcttccacg
      541 tcaatttttg ctcctgtttt gttgtttaag gggccgtttt agccccattt ttggtgaaaa
      601 ttcgaaggaa aatggcatca aaattcgtct tcaatctgct gatccaggtc ggaatcattt
      661 aacggttctg atgctttcgg cactggctca cgggcgattt catgggccgc tcttactact
      721 gccataaggt gatgtgccgc ttccgggtga agaagtaaca ggtcaacggc gtcaatgatg
      781 gcctgtcgtg ctttctcctg tgccaatggg tcactacaac cacgacgcat taagtcctga
      841 acttcagcca gcggtttgac ctgggcgcgt aggtaacgct cacggaatac cggatcgctt
      901 tcgcatttgg tctgaataat gcggtctttc agttccataa tttttcccct tcttagaagt
      961 agtcgttcca cgatggcaca ttggcctgat gatgctgatg gacttttaat ccagcctcag
     1021 ccactttgtt ctcttcactg acctgattgc gcttctgtac ggcggcgttt acgtcctgtt
     1081 gaagctgtag caagtcttct tccgacatgt tgaataattc cgccttgatg gctattaagg
     1141 ctgctttgat gcgttctgcg cggttcagct cagcttccgg gcgaacgtct gctgattgtg
     1201 tcgctacggt tagctcatac tgattcattt gtcgtcacct cccacatcgc agcccactga
     1261 agctaactgc gcgtgtatat cgttacgtat taacttgagt cggcggataa actcgcgttc
     1321 ttcctggttg cactcatgca ttgatgactc gtttttcagt ttcccggcaa taaggcggtt
     1381 gatggttccc agcacaagct cggcaaagca acgaagtgct acccgtttgg ctggctggca
     1441 ttgatacggg cattcgtggc gcaagaagct gacgcgttcc gcgatctctc gtgacatgcc
     1501 gtgtattacc ttaagtgcta acatgtttcc ctctcatttc tgaaagcgag tggcggcagc
     1561 ggtataatgt tcttgaacat tcttcgagcg tcaccactca tgcgttaatg ttcgattggt
     1621 cgcattgttg agattgagcc aggcgcaata cctggctttt tctttgccta gcaagggatg
     1681 ccgatagcgt tcaggatgcg ctttctcaca tcggcatcca tttcatagct cttaggcgtt
     1741 tcagtgctct gacgcgcttc tggcatattg tcgggaacct taactgtgaa gtaatcccgc
     1801 atatcatccg tcagatagtc acctgaaaag taatcaccaa atgccgggtc gagtttattg
     1861 tcgaaataat caagtccggg tactttactt tctgactgct ggccttgttt cggtagcatt
     1921 ttgattattt ccattttgat ttcggccgcg cgtttttgta atgcggcccg ttcggctcca
     1981 gttttctcgg ctgctgctga ttcgaggatc tggaattgct ggacgaggcg ttcaatttcg
     2041 tctttcataa tttgtatccc cggtaatctt atttatttaa ccagttgagg ccacggccca
     2101 atttattcgc taccttcccg gcaagaaaac ggcgacatgc cttttcatcg aaccgctggt
     2161 gtgttaccgg atccggctta cgcaatactt ccggtaccaa atcagccagg cgggcagctt
     2221 caccgtcaat aatatccgcg agcaagtcag gaattaattc aattactcgt tctgctgttt
     2281 tattcatctc ttcatcaaat gcgcgtttat ctaaagcctc ctgtgatggt tgatattttg
     2341 gcatgcggtc aaaataagtg ttagacatgg tattttcctc ttaattagtt agctttctat
     2401 atattgatga ctctacgtga aaaatagaat ttgtcggctg tagttgttcc caatttttaa
     2461 tgtcggcaac ggaacggaag ctgccaatgc tgattaactt tccacaacat cgcagtagtt
     2521 accgggcgaa gacaccagtg cgccttgtgc ttcgtagcgg ccttgcgtgg ctgttcgtgt
     2581 ggcttgcgac gttcggcact gttgacagct acgggcacga tttcaagatt cgataaaaca
     2641 ttgttggtgc cgttgccatc gcgatgatta acacaaaaac ccggtttgat agcgtcgaat
     2701 ccatacgtta accagataac gatgttgtgc acgtagaacc ttttccgttt accgttggct
     2761 cttatattta ccgcgtaata acgatgttta ttgtgacgtt tatatttccg gccccgtctg
     2821 ataacatatt tatgctcaga cggtttcagg cataattcat gaccgtcacg atcgactcca
     2881 gtctggcttt tagcgttaac agtaaaatgt tttctaatct cttcctgata ctctggcggc
     2941 ggaattaaag gtgtcggcat tggcataatt aacgctcctt attccggttc atgtactcga
     3001 taatctcgtg tcgataccat ttctgatgaa tgcctttaac ctgtggtgta aaaggtgaag
     3061 ggaagtcagg aattgtatcg caccacttgg tgatggtttg tcgatgcaca ccaaacatcc
     3121 tcgcgacttc tgaaataccg ataatgtcat tatctttgtt catgatgtat ttccctcaaa
     3181 aaagagagcg gcaactggaa atatgatggc gacggttgcc gcttttagtc ctgaagtaat
     3241 ccaagctaag tggatacctg ttctatgtga tctattttcc cgcttaattc gtatcttcac
     3301 aactttgtgc ggtggctggc ggttatcacc ttttttctat attaatgcgc ggcggctgta
     3361 agtgcccatt ttccgtgcgt ccgatatcac gcttaatcag acatgcggat ccagacggta
     3421 gaatgcggcc tgcgacctgc tgaaatgtgt aacatcgcag gtagttttcc agatcgggct
     3481 atttggtatc gtatgccagt gactgcggac aataaccgtt ggtagccagg ctggcgagta
     3541 aaagatgcgg gcggtcggtg tcatcactgc caggcaataa aaatcatcag ccgtataaac
     3601 gcttcagagc aattctaagc gccttatccc tacggataag caaacggtca actatgccgg
     3661 attgagcgcg ttacaggcgc ggcagtaagc gagtgccata gctgcggcac ataatcatca
     3721 tcgtcgtcat agacaaagca gtacatgcta catcgagcaa tcacagatga aaaaccgggt
     3781 cgggcaggaa ggcgggtgcc gtagcgcaag cggaggcacc aggcatgtgg ctacggtgcg
     3841 gaatacaatt cacaaccgtt taacaaaagc acatacatcg cgataacgcg aaaatgccgg
     3901 gttacacatc cgtaactgtc tgtaacctaa tcagtcaaag ccagacttgg cgcggctctc
     3961 aggccgatta tagcgaattg aaacaccgtt ttggttacac atccgaggta ctttccaaaa
     4021 ctatttaatt tataaaaata gagtaaaagt taagtgaatt cttttttttc tgatttatct
     4081 gtaaccatgt gtaacccata agatatatgt aactatatca ataagttaga caggttacac
     4141 atccggttac acatcggtta cacatccact atctgtaact ttttgcgcaa aaaaaagcca
     4201 accccgaagg attggcatgt taaaaccaga acaatctgtt tacttatttg taacctcgac
     4261 cattacgtcg cttgaggctg ctaacttggc gactccggcc cggtcgcgag cacttcccat
     4321 ttcgcttgag atctggactg gtttcgcatt ttgccattgt tcatggttcc gaattatcca
     4381 gccgcgcacc gcttttccag caagttttac aactggcgtt ggcaccattc cggcatcttc
     4441 aaacatccgg cgctgtttgt tagtgatcgc cgcacccacg tctcccagga tgtgctcagc
     4501 cagatccctg ttgctgatga tgtcagaagg ccagtcagcg atcagttcgt cgagatatac
     4561 gcgctccagc ggtttggcag agttaattgc tcgcaacttg tcttcatcac acacaggcgg
     4621 ctgtttaata tcgaacccac tcaggtcacg cgttgccagt tgccatccta acgcgttaat
     4681 cgccttaggg ttagctaaca ggtcatcaag acgtttcgcc atcaccctgg ctgcctcagg
     4741 gccgtatacg tcagcatatt tgcccgcagt atggcgaatc gcaaaccagc ggcggtcgtg
     4801 ttcgtccagc ggcagcgggt tatcgtggtt agaacagaca aaccagcggc acgcattcca
     4861 ttcgtcatac ttcgcaccgt atttcacgtt aatgtgtcgg ataggctcag taatcatcgc
     4921 cttcagacgg ttagccgtac gatactggct attgtcgccc tcgcggattt catcgcatgt
     4981 agcaagaaca gccccggcta attcgctgtt aaattgtcct tctaacaggt catgaatgtt
     5041 tacaccagat gcgcagttgc gccccagtaa tgctttcagg atttcacaaa atgtcgtgcg
     5101 cccgacgccc tgtataccat tcgtgtagtg gagaatgccc caatgaggca gcacatcagg
     5161 acgctgaatc atgtgcgcaa tccagttaac aacagtcacc cacttatcgc caaataacca
     5221 acggcaatca tcatagaacg cgcttacatc cgtttcatag tccggcgtcc atactggttt
     5281 cttccagaga ttaaggcatt ttcgaccgtt cggatcttcg cagatgacat cacgaccagg
     5341 cgcacaggtc agagcatcaa caatcatgcg tttatcgctc tcacgccata ggctcgtagt
     5401 cggtactgct ttcatcttca ggccaccgcc gcccccgaca tcaatcatcg tcttacttgc
     5461 tgccatcatg ttcctgaatt ctgaccaggc gaagttacgc cacggctcat caagaaacgc
     5521 gactcgctca ccactggcga tccacacgca tttagcaagc atctcatcca gcgccatcac
     5581 tttcgctttc atggtgtcgc ggttaacgcg ctgtggttcc atcgccttta ctgcggcttc
     5641 tgctgcccgc tggcgttcag cttcacgctc tctcgcttgt tgttcggcga tctctacgac
     5701 gctggcaagt tcttcaggag taggacattt gtgctctttg gcagaatcga taggcaccac
     5761 atttgtgctg tctttttcat gttcgccctg gtcaaccggg ctattttgtg gcatattatt
     5821 catgtgagtt tcctccttac cggttgtgat tctcatgtga atttcctcct tgccattgga
     5881 ttttcacagc cgttcgattc agcaccgtta cggcttctac atcgccccgc tcgcaacggg
     5941 gcgttgttat ttctgaagtt ctacacattt tcttccgctc cgttttaaat ctttgatcag
     6001 ttcttctttg tgcatttcgt accactcaag catgtcagcc ttcatccaga tcaacatctt
     6061 gccccttccg ctgttctctg gtttgggaaa atctggataa cgcctcatta cgtcatagat
     6121 tttcgtgcgt gagcgttgca gtagctcgca aacgtcagac attgccatta cgaaatcttt
     6181 tacagtttgc ccttgcatgg tttttaccct ctctgttgaa gtacacgatc acaataacac
     6241 tattttctgt cccgtaacaa ctttgctcac acagcaaaaa acccggtgtt taaaccgggc
     6301 ttcacttgag tcatcgcggt agctgtttat cattctccat cagccgcctt ctgttgaagc
     6361 gttactctga tgcgtcctgc tttacctttg cgttttccgt tttcctcgcg gatctcgtta
     6421 ccatctttat cgtgccggaa tatcatctcg cggtcgtact gaatcagcgc attatcgcgc
     6481 gcttcagcca gtaggcgata gaaacccgcg accggaatac ttttcccttc aatcgtgatt
     6541 agcgactgca atcctgcgtt attgccttcg taaacaccgc caatgttttc cagcgcctga
     6601 atcaataaat caacatgcca tttcttcggc ttcggattgc cacgctccat cggttccggc
     6661 ttgttggaat cttgtttttt atctgttgct acgctgtttt cattctccat cgcctcatta
     6721 gtgatggtga cttgttcttc tgcgtagatg tgtgccagcg ccacacgact taatcccaag
     6781 tgctgaatga cttttgccag ggcaacaata tccgggcgct tgtgtgggcc tttttggtgc
     6841 tgcgtgaata tggcgctaat ttcatttgtg ccgatacgca gttcctgggc gatctgctgg
     6901 tatccgatac caccatgttt gcctggatat ttacgctccg ccgtctcgcg taattgtctg
     6961 taaaacttgt tcatcttttt atcctccatc gctttccgct tgatgaaagg ttaacaccaa
     7021 catatcaagc tcgttagctt tttgacgctg ggtattgacg cagatcacaa aaaaacccgg
     7081 cgcggggccg ggctgttgat tgcgtgcggt tggtgtccta acgttcccat aattgcccct
     7141 tacaattcca cacagcaaat catcatcacg ataatcaaca agataagctc ataccgtttc
     7201 attgtttcat cctccatcga tttccgtctg ttggagagtt catccgttga ttgaattaaa
     7261 caaaaatgcc gggcctgata aagagatcta catcacaaaa acaactctcc atcaggaaaa
     7321 acacgttgga acatgattaa tggtgattta ttaagcgaaa tgatacttca tcgcagatta
     7381 acggttggag ggttataata agcgggcaat cgggggattg tgagagggta caagaaaggg
     7441 gaacaggcta acgcttgctc ccctttgttt tgcggttagt tgatggcttt aacgcggaag
     7501 ttttccggta gcttgccctc tttgatggcc tggtcacgga tacggtaaac gcttgtccgg
     7561 ctgatcttgt gcgcacgggc aatcttcgct ggctggtgac cgtttttgag cgcctcgata
     7621 acaaattctt cgctctcctg gcgacgcttg cttttacctc gaccaatctg cttacctgcg
     7681 gctttcgcgg ctgcgtacgc ttccgcctga cgctctaaca tgatgtttct ctccagttgg
     7741 gctaacactc ccatcatctg gaagaacgcc tcctgtaacg cgtttttctc agcgcccagc
     7801 acgtaacctt ctttcaggca atggagaaca acgccgcgtt ctctcaggtc ttcaaccatt
     7861 ttcagaatcc cgataaggtt gcgccccaaa cggtccaggc tggcaacgta tacgtggtca
     7921 ccttctctgg catagtcgag catcagcttc agttgttcgc ggtcctcgtt cttgccggac
     7981 tttttctctt caaaccactt atcgatcgca atgtctttca tagcgtgata ctggcggtca
     8041 gtattctgct gaatagttga tacgcgaatg taagcgaagt tagccataac agcctccagt
     8101 gttcaaatac ccttgcttct tcattacgga cacattaata ccaaataccc acacacgaaa
     8161 ggcatttgtt acagtttttt gacgttcatc aagcaaaaac aggctgttca atcaaacatg
     8221 gttgcggaca ccatcggatc agacgcttct acgcgaaaaa tggaaatgtt ggcgggtagc
     8281 tggaagggaa tttttaagca ggtgcgtaaa tgtcgcaaaa cgcgacgttt gaatggaccg
     8341 ccagtctgtg tgcgccgctg gcgtcggcta cgcacgcgta cttgtccgaa aactaaattt
     8401 gctccggcgc ttgcagcacg taatcttccg gtacaaacat gccttcgcaa tcggcgtgag
     8461 ggttgtcgct gtagtccggt cgggcacgaa tgttagccag tgcgataccc tggaggtcaa
     8521 cggcgtcata gccaccaact ggcagatctt cattcaatcg cttctcattg tcgcggacca
     8581 tgcgccagta ccgggcgcgg tcggttgctt tgaatgtgtc tttgtagtcc aggctatgga
     8641 cggcaataac tttgacgtat cccgtccaca tataagcgaa tgccggatcg gtcagtaccg
     8701 ccttgttatg aatcatcccc ggcagtggcg gcgctatccg gcgcatacag aggcagttgt
     8761 ctatgatagc tacttcgtgg ttgtttatct tgtcggccat ttctaacagc gccatttggc
     8821 tcccttgttc gtagttggtg gtgtacagtt ccagtgccac gtcgatggtg cca
//
