LOCUS       CP058003                1306 bp    DNA     circular BCT 13-MAY-2022
DEFINITION  Escherichia coli strain RHB06-C03 plasmid pRHB06-C03_8, complete
            sequence.
ACCESSION   CP058003
VERSION     CP058003.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15148008
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   AbuOun,M., Jones,H., Stubberfield,E., Gilson,D., Shaw,L.P.,
            Hubbard,A.T.M., Chau,K.K., Sebra,R., Peto,T.E.A., Crook,D.W.,
            Read,D.S., Gweon,H.S., Walker,A.S., Stoesser,N., Smith,R.P.,
            Anjum,M.F. and On Behalf Of The Rehab,Consortium.
  TITLE     A genomic epidemiological study shows that prevalence of
            antimicrobial resistance in Enterobacterales is associated with the
            livestock host, as well as antimicrobial usage
  JOURNAL   Microb Genom 7 (10) (2021)
   PUBMED   34609275
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Shaw,L.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2020) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Assembly Name                     :: RH14 T1-C03
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 89.5791523127404x
            Sequencing Technology :: Hybrid: Illumina and PACBIO_SMRT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/19/2020 13:55:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,950
            CDSs (total)                      :: 4,831
            Genes (coding)                    :: 4,722
            CDSs (with protein)               :: 4,722
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 51 of 109
            Pseudo Genes (incomplete)         :: 40 of 109
            Pseudo Genes (internal stop)      :: 32 of 109
            Pseudo Genes (multiple problems)  :: 14 of 109
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB06-C03"
                     /isolation_source="Pooled sheep faecal samples collected
                     from floor of farm"
                     /db_xref="taxon:562"
                     /plasmid="pRHB06-C03_8"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(427..963)
                     /locus_tag="HVV91_24750"
     CDS             complement(427..963)
                     /locus_tag="HVV91_24750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QMQ11822.1"
                     /translation="MQTQNVITEKEREIRSRDLTENAKKLAHERREAEKNSNFTQVSPL
                     GWKRLMELAKGNQGAFGLYSFFAQHIDGSCGAVVADQSFLAAQFNVSTRTIIRWINYLE
                     ENRAVVRIPVAGKVCAYALNPNEVWKGYGNTKEYAAFITKTLVNKDGDIQRRIMAMFHG
                     NDARQGMEGDTPEAE"
                     /name="helix-turn-helix domain-containing protein"
ORIGIN
        1 cggtcgaaaa aaatagggga aggtggttgt gttcacgcag tgagcgcaac cacctgccag
       61 acgggttttc gcgccgcagg ccgcacatgt ttctgagcca acggcgaaga aacatataag
      121 tgcgcatctc tctaattttg cgaatttgag agatgaaacc agtctggggg atcggagtat
      181 tccgcctcta gccgtcctgg tggcccagtc cggcaagacc cacgaaatca cccgtaaaac
      241 gccctaaaac gcgctctgac gcgttttttg acgttttacg ggctcacgtg ggctttgggc
      301 ggaaaaatgg cttacgtgcg ttctgagagc gttttagggc tttgcagggc ggagggggtg
      361 aagcagctcc ctgtggaacc tgtaccgccc ttgcgggcgg gcttcggcga tttggcgaac
      421 gggaatctat tcggcttctg gcgtgtcgcc ttccatgccc tgacgggcat catttccgtg
      481 gaacatggcc atgattcggc gctgaatgtc gccgtctttg ttgactaggg tttttgttat
      541 aaacgccgca tattccttgg tattcccata ccccttccaa acttcatttg gattcaatgc
      601 gtatgcacaa accttaccgg cgacaggaat cctcacaaca gccctgtttt cctctaaata
      661 gttgatccat ctaattattg ttcttgtcga tacattgaac tgcgccgcca aaaatgactg
      721 atccgccact actgcgccac aagatccatc gatatgctga gcaaaaaaag aatacaagcc
      781 aaaagctccc tgatttcctt tggctaattc cattaaacgc ttccagccta atggggacac
      841 ttgtgtgaaa tttgagttct tttctgcctc tcgcctctca tgagccagtt ttttggcatt
      901 ttcggttaga tccctagatc gaatttctcg ctctttttcg gtgattacat tttgagtttg
      961 catctcaatc tccatgtcat ttcaatgtga caagataagt catactatac gacattttat
     1021 gtcgtagtat atgtcattta tgtcacatat tacgaaatga tgtgacatcc tagggtgtca
     1081 taacgttgtg acatcctagg gtgtcatatc agaaatgtta aatgtctttt aaatcaatga
     1141 attattaatt tttgctattc ttatctaaag agatctcaca gcgcgctgcg agatctcctg
     1201 gtagcaccct cctttcagtc gggcaaacgg cgaaaattta accattggca accgaaaaat
     1261 cgtgggggcc ccttcagggg ttccgatttt tttgttgcca acgcac
//
